Lauper Instruments Mocon Ametek OX-TRAN 2/22 User manual

OX-TRAN Model 2/22 Operator’s Manual About This Manual
MOCON, Inc. Revision R i
Revision R
Part Number 143-195
OX-TRAN®
Model 2/22
Operator’s Manual
Gas-Detection
Lauper Instruments AG
Irisweg 16B
CH-3280 Murten
Tel. [phone]
[email protected]
www.lauper-instruments.ch

Safety Information OX-TRAN Model 2/22 Operator’s Manual
ii Revision R MOCON, Inc.
Trademarks & Patents
MOCON, COULOX, and OX-TRAN are registered trademarks of Mocon, Inc.
For information on applicable patents see:
https://www.ametekmocon.com/aboutus/legal/intellectualproperty
Copyright© 2021 MOCON, Inc. All rights reserved.

OX-TRAN Model 2/22 Operator’s Manual About This Manual
MOCON, Inc. Revision R iii
About This Manual
This manual explains how to set up and use the OX-TRAN Model 2/22 Oxygen Transmission Rate System.
This manual is designed for viewing electronically. Most references to other sections or chapters in this
document are hyperlinks which can be used to navigate to the referenced section.
Please use the following guide to get started with the system.
If You Want To Set Up the OX-TRAN Model 2/22
1. Prepare your test site.
See Appendix A “Site Preparation Instructions” starting on page A-1 in this manual.
2. Install the OX-TRAN components.
See Chapter 2 “Setting Up” starting on page 2-1 in this manual.
If You Want Some Background on How the System Works:
1. Get an overview of the system.
See Chapter 1 “Introduction” on page 1-1 in this manual.
2. Learn how the OX-TRAN measures oxygen transmission.
See Appendix D “Theory of Operation” on page D-1 in this manual.
3. Learn about the software.
See Chapter 4 “Using the Instrument Software” starting on page 4-1 in this manual.
If You Want To Get Started Measuring Oxygen Transmission Rate:
See Chapter 3 “Preparing for a Test” beginning on page 3-1, Chapter 5 “Testing Flat Films”
on page 5-1 and Chapter 6 “Testing Packages” on page 6-1.
If You Have Problems Operating the System:
1. If an error message appears on instrument screen see Chapter 8 “Troubleshooting”
beginning on page 8-1 in this manual.
2. If no error message appears but you have other problems, see Chapter 8 “Troubleshooting”
beginning on page 8-1 in this manual.
SERVICE NOTE:
Please do not hesitate to call MOCON in the USA at [phone]. We want you to receive the
best in product support services.

Safety Information OX-TRAN Model 2/22 Operator’s Manual
iv Revision R MOCON, Inc.
Safety Information
Be sure to read and understand this section and all other applicable chapters of the Operator's Manual and all
on-product safety signs before setting up, operating, and maintaining this analyzer.
Safety signs appear in this manual and on the analyzer. All safety signs are identified by the words WARNING and
CAUTION. These words signify the following:
•WARNING indicates a potentially hazardous situation which, if not avoided, could result in death or
serious personal injury.
•CAUTION indicates a potentially hazardous situation which, if not avoided, may result in moderate
personal injury and/or possible damage to the analyzer and its components.
To avoid personal injury and equipment damage, observe the following precautions:
Installation Precautions
•Use appropriate precautions when lifting or moving the instrument. A two-person lift is recommended.
•The maximum pressure applied to the instrument from the Carrier and Test Gas tanks must not
exceed 29 psi (2 bar), or damage to the instrument will occur.
Operating Precautions
•To avoid plumbing contamination use only specified gas types at recommended operating pressures.
•Use only HPLC-grade water in the humidity generators.
•Use care to avoid splashing the RH sensor with any salt solution. The RH sensors can be irreparably
damaged by direct contact with salt solutions.
•Handle the RH probe carefully. The sensor housed in the probe is easily damaged.
•Do not expose the RH probe to an environment with RH greater than 90% RH.
Maintenance and Service Precautions
WARNING! HAZARDOUS VOLTAGES ARE PRESENT INSIDE THIS INSTRUMENT. SERVICE
SHOULD BE PERFORMED BY QUALIFIED PERSONNEL ONLY.
AVERTISSMENT: CET INSTRUMENT PRESENTE DES NIVEAUX DANGEREUX DE TENSION.
L'ENTRETIEN DOIT ETRE EFFECTUE PAR UN PERSONNEL QUALIFIE
UNIQUEMENT.
WARNING! Protection may be impaired if this device is not used in the manner specified.
•Maintenance and service should only be performed by qualified personnel.
•Make sure the instrument is powered OFF and unplugged from the power source before removing the
covers to perform any internal maintenance or service. Failure to do so can result in electrical shock,
which can cause injury or death.
•Failure to use proper safety equipment when mixing salt solution can result in frostbite or burn injuries.
Salt solutions should be prepared only by qualified people with proper safety equipment.

OX-TRAN Model 2/22 Operator’s Manual Table of Contents
MOCON, Inc. Revision R v
Table of Contents
About This Manual ........................................................................................................................................iii
Safety Information........................................................................................................................................ iv
Chapter 1: Introduction..............................................................................................................................1-1
Features...............................................................................................................................................1-2
Permeation Test Overview ...................................................................................................................1-3
Individual Zero Phase.....................................................................................................................1-3
Conditioning the Sample ................................................................................................................1-3
ReZero State..................................................................................................................................1-3
Cell Examination State ...................................................................................................................1-3
Bypass State ..................................................................................................................................1-3
Test Completion .............................................................................................................................1-3
Report Generation..........................................................................................................................1-3
Measuring an Oxygen Transmission Rate............................................................................................1-4
Testing Films and Packages ................................................................................................................1-4
Chapter 2: Setting Up ...............................................................................................................................2-1
Unpacking the System .........................................................................................................................2-1
Preparing for Installation ......................................................................................................................2-1
Front Panel Parts and Controls ............................................................................................................2-2
Back Panel Parts and Controls.............................................................................................................2-4
Test Gas (Oxygen)...............................................................................................................................2-5
Carrier Gas (Nitrogen)..........................................................................................................................2-5
Gas Distribution Systems .....................................................................................................................2-5
Connecting the Gas Lines ....................................................................................................................2-6
Setting the Gas Supply Pressures........................................................................................................2-8
System Outgassing ..............................................................................................................................2-8
Chapter 3: Preparing for a Test.................................................................................................................3-1
Testing Basics......................................................................................................................................3-1
How Barrier Properties Affect Testing ..................................................................................................3-1
Testing Very Good Barriers ............................................................................................................3-1
Testing Very Poor Barriers .............................................................................................................3-1
Testing Mixed Barriers....................................................................................................................3-2
Testing Moisture Sensitive Barriers ................................................................................................3-2
Developing Test Methods for your Films and Packages.......................................................................3-2
The Examination Time....................................................................................................................3-2
The ReZero Frequency ..................................................................................................................3-2
The Sample Conditioning Time ......................................................................................................3-3
Individual Zero Processing .............................................................................................................3-3
Determining When to Stop a Test...................................................................................................3-4
Choosing a Test Method ......................................................................................................................3-4
The Auto Test Method ....................................................................................................................3-4
The Advanced Test Method............................................................................................................3-4
Saved Test Methods ......................................................................................................................3-4
Using Test Sequencing ........................................................................................................................3-4
Chapter 4: Using the Instrument Software.................................................................................................4-1
Software Features................................................................................................................................4-1
Instrument Software Structure and Organization..................................................................................4-2

Table of Contents OX-TRAN Model 2/22 Operator’s Manual
vi Revision R MOCON, Inc.
Chapter 5: Testing Flat Film Samples .......................................................................................................5-1
Testing Suggestions.............................................................................................................................5-1
Preparing for a Film Test......................................................................................................................5-2
Sample Size.........................................................................................................................................5-2
Using Masks ........................................................................................................................................5-2
Orienting the Sample ...........................................................................................................................5-3
Humidified Testing ...............................................................................................................................5-4
Filling the Humidifiers...........................................................................................................................5-4
Filling the Humidifiers during a Test .....................................................................................................5-4
Mounting a Sample in the Test Cell......................................................................................................5-6
Loading and Unloading the Test Cell....................................................................................................5-7
Setting Up and Starting a Film Test......................................................................................................5-8
Monitoring and Controlling a Film Test .................................................................................................5-8
Chapter 6: Testing Packages....................................................................................................................6-1
Testing Suggestions.............................................................................................................................6-1
Preparing the Instrument for a Package Test .......................................................................................6-2
Package Testing Methods....................................................................................................................6-3
Testing using Ambient Conditions ..................................................................................................6-3
Package Conditioning.....................................................................................................................6-3
Testing using an Environmental Chamber......................................................................................6-3
Package Mounting Methods.................................................................................................................6-4
Mounting Bottles, Cups and Trays using a Package Plate..............................................................6-4
Mounting Pouches and Bags..........................................................................................................6-5
Supplying a Test Gas...........................................................................................................................6-6
Using Ambient Air for the Test Gas ................................................................................................6-6
Using a Compressed Gas for the Test Gas ....................................................................................6-6
Setting Up and Starting a Package Test...............................................................................................6-8
Monitoring and Controlling a Package Test ..........................................................................................6-8
Chapter 7: Maintenance............................................................................................................................7-1
Cleaning the Instrument .......................................................................................................................7-1
Cleaning the Air Filters.........................................................................................................................7-2
Cleaning the Test Cells ........................................................................................................................7-3
Maintaining the Test Cells ....................................................................................................................7-3
Drying Out the Humidifiers ...................................................................................................................7-3
Test Cell Components and Part Numbers ............................................................................................7-4
Maintaining the Oxygen (Coulox) Sensor .............................................................................................7-5
Verifying Oxygen Sensor (Coulox) Performance ............................................................................7-5
Replacing the Coulox Sensor .........................................................................................................7-5
Maximizing Coulox Sensor Life ......................................................................................................7-5
System Standby...................................................................................................................................7-6
System Shutdown for Storage or Repair ..............................................................................................7-6
Chapter 8: Troubleshooting.......................................................................................................................8-1
Error Messages and Warnings .............................................................................................................8-1
Troubleshooting Testing Problems .......................................................................................................8-1
Recovering from a Sensor Over-Range Condition................................................................................8-2
Recovering from a Power Failure .........................................................................................................8-2
Chapter 9: Specifications ..........................................................................................................................9-1
Physical Specifications.........................................................................................................................9-1
Environmental Requirements ...............................................................................................................9-1
Electrical Requirements .......................................................................................................................9-1
Gas Supply Requirements ...................................................................................................................9-2

OX-TRAN Model 2/22 Operator’s Manual Table of Contents
MOCON, Inc. Revision R vii
Environmental Chamber Specifications................................................................................................9-2
Operational Capabilities .......................................................................................................................9-3
Appendix A: Site Preparation Instructions ................................................................................................ A-1
Appendix B: Spare Parts.......................................................................................................................... B-1
Appendix C: Warranty and Service Policies ............................................................................................. C-1
Appendix D: Theory of Operation............................................................................................................. D-1
How the Oxygen Sensor Works .......................................................................................................... D-1
How the Transmission Rate is measured ............................................................................................ D-1
Factors that Affect the Transmission Rate of a Barrier Material........................................................... D-2
Barrier Temperature ...................................................................................................................... D-2
Test Gas Driving Force.................................................................................................................. D-2
Barometric Pressure...................................................................................................................... D-2
Relative Humidity .......................................................................................................................... D-2
How a Humidified Test Gas is Generated............................................................................................ D-3
How the ReZero Cell Works................................................................................................................ D-3
How the Auto-Test Method Works....................................................................................................... D-4
How Sequential Testing Works ........................................................................................................... D-4
Appendix E: Electrical and Plumbing Diagrams ....................................................................................... E-1
Appendix F: Preparing RH Calibration Standards .....................................................................................F-7
RH Calibration Equipment....................................................................................................................F-7
Choosing a Salt Solution ......................................................................................................................F-7
Preparing a High Level Calibration Standard........................................................................................F-8
Preparing a Low Level Calibration Standard ........................................................................................F-8
Preparing a Saturated Salt Solution .....................................................................................................F-8
Appendix G: Permeant Sensor Adjustment .............................................................................................. G-1
Permeant Sensor Performance Check ................................................................................................ G-1
Performing a Permeant Sensor Adjustment ........................................................................................ G-1
Permeant Sensor Adjustment Test Setup ........................................................................................... G-2
Appendix H: Compliance.......................................................................................................................... H-1

Table of Contents OX-TRAN Model 2/22 Operator’s Manual
viii Revision R MOCON, Inc.
Figures
Figure 1-1: OX-TRAN Model 2/22 System ................................................................................................1-1
Figure 1-2: The OX-TRAN Model 2/22 Test Cell .......................................................................................1-4
Figure 2-1: Front Panel Parts and Controls ...............................................................................................2-2
Figure 2-2: Front Panel Parts and Controls ...............................................................................................2-3
Figure 2-3: Back Panel Parts and Controls ...............................................................................................2-4
Figure 2-4: System Plumbing Connection Diagram...................................................................................2-5
Figure 5-1: Humidifier Fill Port and Controls..............................................................................................5-5
Figure 5-2: Mounting a Film Sample .........................................................................................................5-6
Figure 5-3: Loading the Test Cell ..............................................................................................................5-7
Figure 6-1: Installing the Package Adapter Cell ........................................................................................6-2
Figure 6-2: Using a Package Mounting Plate ............................................................................................6-4
Figure 6-3: Pouches and Bags - Bottom Mount.........................................................................................6-5
Figure 6-4: Pouches and Bags - Top Mount..............................................................................................6-5
Figure 6-5: Supplying a Test Gas..............................................................................................................6-7
Figure 7-1: The Air Filter ...........................................................................................................................7-2
Figure 7-2: Test Cell Components ............................................................................................................7-4
Figure E-1: Plumbing Diagram Page 1................................................................................................... E-2
Figure E-2: Plumbing Diagram Page 2................................................................................................... E-3
Figure E-3: Electrical Diagram Page 1................................................................................................ E-4
Figure E-4: Electrical Diagram Page 2 ................................................................................................... E-5
Figure E-5: Electrical Diagram Page 3................................................................................................ E-6
Tables
Table 2.1: Front Panel Parts and Controls ................................................................................................2-3
Table 2.2: Back Panel Parts and Controls.................................................................................................2-4
Table 5.1: Humidifier Port and Controls ....................................................................................................5-5
Table 7.1: Test Cell Components..............................................................................................................7-4
Table 9.1: Physical Specifications.............................................................................................................9-1
Table 9.2: Environmental Requirements ...................................................................................................9-1
Table 9.3: Electrical Specifications............................................................................................................9-1
Table 9.4: Gas Supply Specifications........................................................................................................9-2
Table 9.5: Environmental Chamber Specifications ....................................................................................9-2
Table 9.6: Operational Capabilities - Ox-Tran 2/22 Model H .....................................................................9-3
Table 9.7: Operational Capabilities - Ox-Tran 2/22 Model L......................................................................9-3
Table 9.8: Operational Capabilities - Ox-Tran 2/22 Model 10X..................................................................9-4
Table F.1: Salt Solution Humidity Standards in Percent RH ......................................................................F-7

OX-TRAN Model 2/22 Operator’s Manual Introduction
MOCON, Inc. Revision R 1-1
Chapter 1:Introduction
This chapter provides a brief overview of the OX-TRAN Model 2/22 Oxygen Transmission Rate System.
Read this chapter to get an overview of:
•The features of the OX-TRAN Model 2/22 system
•A summary of basic steps in a permeation test
•A summary of how a Oxygen Transmission Rate measurement is performed
•A summary of how Film and Package testing is performed
Figure 1-1: OX-TRAN Model 2/22 System

Introduction OX-TRAN Model 2/22 Operator’s Manual
1-2 Revision R MOCON, Inc.
Features
The following features of the OX-TRAN Model 2/22 Oxygen Transmission Rate System make it very
versatile and easy to use:
•Fully automatic (Hands Off) testing.
•Reduced test times (less total time to achieve a Test Result).*
•Improved Repeatability specification (2.5 times improvement over OX-TRAN 2/21 H).
•Enhanced patented, humidified and low maintenance Coulox oxygen sensor.
•Removable test cell for faster film mounting.
•Pneumatically clamped test cells.
•TruSeal™ test cells for increased performance.
•Automatic control of the Test Cell Temperature.
•Automatic control of Pressure, Flow and RH.
•Automatic Barometric Pressure compensation.
•Package Testing Adapter (Optional).
•Carrier Purge feature for package testing.
•Fast changeover from Wet to Dry testing.
•Advanced oxygen sensor over-range protection.
•Advanced Gas Saver function reduces gas consumption.
•Advanced automatic testing features simplify testing Barriers with unknown properties.
•Automatic Test Sequencing (perform a sequence of tests at different RH and temperatures).
•Automatically save and print Test Results.
•Internal storage of at least 100 Test Results.
•Integrated and intuitive touch screen operator interface.
•Remote Access and Control (requires optional Perm-Net Light software).
•Standard Ethernet port (100T).
•Dual USB 2.0 ports (for connection of keyboard, mouse or memory devices).
*Compared to the OX-TRAN Model 2/21 series

OX-TRAN Model 2/22 Operator’s Manual Introduction
MOCON, Inc. Revision R 1-3
Permeation Test Overview
A “Permeation Test” consists of up to two phases that are performed using a Test Cell. The “Cell” may
contain a flat film sample or be connected to a package fixture. The Test Phase (always present) is used to
measure the transmission rate of the test sample. The Individual Zero Phase is optional and may occur at
the beginning or end of a Permeation Test. Each phase in the test consists of a series of discrete states. A
Permeation Test may utilize all or only a subset of the possible test states. During a test the steps (or
states) within each phase are executed in a fixed order. This order is called the “Test Sequence”. A brief
description of the phases and states within a permeation test are given below:
Individual Zero Phase
An Individual Zero Phase is used to determine the amount of oxygen that is present in the carrier gas from
factors other than actual transmission through the sample. This amount is subtracted from the transmission
rate obtained for each sample during testing. For more information on when and how to use an Individual
Zero Phase see “Individual Zero Processing” in Chapter 3.
Conditioning the Sample
Almost every material requires a period of time to acclimate to the environment and reach equilibrium. A
time period can be specified to allow the barrier to “Condition” to the test environment. The Conditioning
Period when used will occur at the beginning of each test phase.
ReZero State
Periodically the instrument baseline must be measured so that any changes in the baseline will not affect
the accuracy of your transmission rate data. This is done during a “ReZero State” by measuring the
apparent oxygen transmission rate of the ReZero Cell.
Cell Examination State
The Cell Examination State is used to measure the transmission rate of the barrier sample. The carrier gas
leaving the Test Cell is sent to the oxygen sensor where the amount of oxygen contained in the gas is
measured to determine a transmission rate.
Bypass State
When no gas is being routed to the oxygen sensor it is said to be in the Bypass State. The Bypass State is
used to maximize the useful life of the sensor. The oxygen sensor is automatically placed in Bypass during
conditioning, when tests are completed or when an excessively high level of oxygen is detected.
Test Completion
At some point sufficient measurements will have been made to accurately determine the transmission rate
of the samples. A Permeation Test can be terminated manually by the user, after a fixed number of test
cycles or using an automatic convergence process. When testing is complete, the cell is removed from the
test sequence and no further transmission rate examination of that sample is performed. For more
information on stopping a transmission rate test see “Determining When to Stop a Test” in Chapter 3.
Report Generation
You can request a report for one or both cells at any time until a new permeation test is started. See the
instrument Help System for information on printing reports.

Introduction OX-TRAN Model 2/22 Operator’s Manual
1-4 Revision R MOCON, Inc.
Measuring an Oxygen Transmission Rate
To make an accurate transmission rate measurement a known concentration Test Gas (usually 100%
Oxygen) is applied to one side of the barrier material to be tested and the other side is swept with an
oxygen free Carrier Gas (usually a nitrogen/hydrogen mix). This process is illustrated in Figure 1-2 which
depicts a Film sample mounted in a standard OX-TRAN model 2/22 Test Cell.
As shown in the illustration the film is mounted between the two parts of the test cell. The o-rings and
sealing surfaces in the cell prevent outside air from affecting the measurement.
The Test Gas (oxygen) is continuously admitted to bottom half of the cell and allow to exit through an
exhaust port.
The Carrier Gas (nitrogen and hydrogen) passes through a catalyst to remove any residual oxygen and
then enters the top half of the cell. As Test Gas permeates the sample barrier it mixes with the Carrier Gas.
The output side (exhaust) of the test cell is routed to an oxygen sensor. The amount of oxygen in the carrier
gas is measured using the patented Coulox oxygen detector.
Seal leakage can be a significant source of error when measuring the transmission rate of a film sample.
The TruSeal™ flush ring at the perimeter of the Test Cell minimizes the effect of ambient oxygen on the
measurement. During a test the TruSeal™ flush ring is purged with carrier gas minimizing the possibility of
leakage past (or permeation through) the seals.
Figure 1-2: The OX-TRAN Model 2/22 Test Cell
Testing Films and Packages
The OX-TRAN model 2/22 can measure the oxygen transmission rate of film samples or entire packages.
To test a film sample it is prepared and mounted in a Test Cell. The test cell is used to maintain the film
sample at a specified temperature. The Test Gas and Carrier Gas supplied to the test cell can be Dry or at a
specified RH. For more information on testing Flat Film samples see Chapter 5.
To test packages at ambient temperature; a package mounted in an appropriate “fixture” can be connected
to the OX-TRAN model 2/22 using the optional Package Adapter. For more control over sample
temperature an Environmental Chamber can be used. For more information on testing packages see
Chapter 6.
Flush Gas In
Flush Gas Out
Carrier In
Carrier Out
Test Gas Out
Test Gas In

OX-TRAN Model 2/22 Operator’s Manual Setting Up
MOCON, Inc. Revision R 2-1
Chapter 2: Setting Up
This chapter provides information on how to set up an OX-TRAN Model 2/22 and prepare it for use.
Read this chapter to learn about:
•Unpacking the System
•Preparing for System Installation
•Front Panel Parts and Controls
•Back Panel Parts and Controls
•Connecting oxygen and nitrogen gas lines to the instrument
Unpacking the System
Each OX-TRAN Model 2/22 instrument is shipped in its own crate. Any optional kits or accessories may be
shipped in the instrument crate or in separate cartons. A checklist will be included for the instrument and
any of the optional kits you may have purchased.
If any components are missing or damaged please call MOCON in the USA at [phone].
Preparing for Installation
The Site Preparation Instructions (Appendix A) contain important information about preparing a location for
the instrument and the facilities required for your new instrument. Please read Appendix A before
proceeding with the installation of the OX-TRAN Model 2/22.
The ambient environmental conditions in which the instrument will be used must meet those specified on
page 9-1 in Chapter 9.
The OX-TRAN Model 2/22 should be placed on a bench or table capable of supporting approximately 43 kg
(95 lbs). The work surface should be flat, clean and free of excessive vibration.
If installing a printer that will be directly connected to the OX-TRAN Model 2/22, place it next to the
instrument. Read the manual that came with the printer for installation procedures.
Do not plug the instrument or printer into a power source until all components have been set up and
connected.
Before proceeding with the setup or operation of the instrument familiarize yourself with the locations and
names of the parts and controls on the front and rear of the instrument.

Setting Up OX-TRAN Model 2/22 Operator’s Manual
2-2 Revision R MOCON, Inc.
Front Panel Parts and Controls
The names and locations of the parts and controls located on the front of the instrument are shown in
Figure 2-1, Figure 2-2 and Table 2.1.
Figure 2-1: Front Panel Parts and Controls
6
5
4
3
2
1
8
7

OX-TRAN Model 2/22 Operator’s Manual Setting Up
MOCON, Inc. Revision R 2-3
Figure 2-2: Front Panel Parts and Controls
Item
Name
Description
1 Cell B Load/Unload button This button is used to Load and Unload the Test Cell
2 Cell B Status Indicator This indicator is used to show the Status of Cell B
3 Cell A Load/Unload button This button is used to Load and Unload the Test Cell
4 Cell A Status Indicator This indicator is used to show the Status of Cell A
5 Cover The cover that is used to access the Humidifiers and cell vents
6 Instrument Display The color touch-screen display used for instrument control
7 Test Cell Drawer - Cell A The drawer where the Test Cell for Cell A is contained
8 Test Cell Drawer - Cell B The drawer where the Test Cell for Cell B is contained
9 Model H & L
ReZero
Cell Vent
The carrier gas vent for the ReZero cell
Model 10x Cell A, B &
ReZero
Vent
The carrier gas vent for Cell A, Cell B and the ReZero
10 Cell A Vent
The carrier gas vent for Test Cell A (
Not present on 10x)
11 Water Reservoir - Carrier Gas Window showing the Carrier Gas Reservoir water level
12 Cell B Vent
The carrier gas vent for Test Cell B (
Not present on 10x)
13 Carrier Gas Reservoir Drain/Fill Valve The screw used to open & close the reservoir Drain/Fill valve
14 Thermometer Well A thermometer well for use with a external thermometer
15 Test Gas Reservoir Drain/Fill Valve The screw used to open & close the reservoir Drain/Fill valve
16 Reservoir Drain/Fill Port The Drain/Fill port for the Reservoirs
17 Water Reservoir - Test Gas Window showing the Test Gas Reservoir water level
Table 2.1: Front Panel Parts and Controls
17
15
16
13
11
9
10
12
14

Setting Up OX-TRAN Model 2/22 Operator’s Manual
2-4 Revision R MOCON, Inc.
Back Panel Parts and Controls
The names and locations of the parts and controls located on the back of the instrument are shown in
Figure 2-3 and Table 2.2.
Figure 2-3: Back Panel Parts and Controls
Item Name Description
1 Power In The connector the power cord attaches to.
2 Fuse Holder The fuse holder contains the fuses used to protect the instrument.
3 Power Switch
The switch that is used to turn the instrument on and off.
l is ON
О is OFF
4 Test Gas Inlet Port The fitting that connects the test gas supply to the instrument.
5 Carrier Gas Inlet Port The fitting that connects the carrier gas supply to the instrument.
6 Cooling Fan The fan that supplies cooling air to the test cell heating/cooling system.
7 Network Port A 10/100T Ethernet port for connection to a network.
8 USB Ports USB 2.0 Ports for connection of USB devices (keyboard, mouse).
9 Enclosure Fans The fans that supply cooling air to the instrument enclosure.
Table 2.2: Back Panel Parts and Controls
5
4
3
2
1
6
7
8
9

OX-TRAN Model 2/22 Operator’s Manual Setting Up
MOCON, Inc. Revision R 2-5
Test Gas (Oxygen)
The OX-TRAN Model 2/22 requires an oxygen test gas with a purity of at least 99.9%. The regulator (on the
compressed gas cylinder) must be approved for oxygen use. The optional Regulator Assembly contains an
approved Oxygen Regulator. A standard T size cylinder should provide sufficient gas to operate a single
instrument for several weeks.
Carrier Gas (Nitrogen)
The OX-TRAN Model 2/22 requires a Carrier Gas mixture that is 98% nitrogen and 2% hydrogen. The
specific percentage of each gas is not critical. The percentage of hydrogen in the mixture may be as low as
1% or as high as 3%. The hydrogen is used with a catalyst in the instrument to remove any residual oxygen
in the Carrier Gas.
Compressed gas cylinders containing hydrogen require a “Hydrogen Regulator”. The tank fitting on a
Hydrogen Regulator uses left hand threads. The optional Regulator Assembly contains an approved
Hydrogen Regulator. A standard T size cylinder should provide sufficient gas to operate a single instrument
for several weeks.
Gas Distribution Systems
Each instrument ships with two local regulator/isolation devices (called Regulator Tees) that can be used to
connect the instrument to a common Test Gas/Carrier Gas distribution system. Figure 2-4 illustrates how a
gas distribution system for multiple instruments can be created using the provided Regulator Tees.
When using compressed gas cylinders, consideration should be given to the impact cylinder replacement
will have on operational efficiency. Interruptions to the gas supply when a cylinder is changed (or goes
empty) will have an adverse effect on any active tests. Cylinder manifolds that provide an uninterrupted gas
supply during cylinder replacement are available from many gas suppliers.
Figure 2-4: System Plumbing Connection Diagram

Setting Up OX-TRAN Model 2/22 Operator’s Manual
2-6 Revision R MOCON, Inc.
Connecting the Gas Lines
The OX-TRAN Model 2/22 has two gas inlets, one for connecting the Test Gas (oxygen) supply and the
other for connecting the Carrier Gas (nitrogen/hydrogen) supply. These compression fittings are intended
for use with 1/8” copper tubing. Refer to Figure 2-3 and Figure 2-4 for the location of these fittings and an
example of plumbing system diagram.
The optional Starter Kit (051-939) contains 50 feet (14 meters) of tubing in addition to extra compression
nuts, ferrules and grease.
Follow these steps to connect the Carrier Gas supply lines:
1. Remove the brass plug from the Carrier Gas fitting on the instrument back panel. Save the
plug it will be needed later.
2. Cut a piece of tubing approximately 3 inches in length (33 cm).
3. Cut a piece of tubing approximately 10 inches in length (33 cm).
4. Apply a thin coating of Apiezon grease to four compression ferrules.
5. Connect the Carrier Gas fitting on the instrument back panel to the Brass Filter (051-791)
using the 3 inch length of tubing and two nuts and ferrules. The arrow on the Filter must
point towards the tube connected to the instrument back panel.
6. Connect the input on the Filter to the output fitting on a regulator tee using the 10 inch length
of tubing and two nuts and ferrules.
7. Apply a thin coating of Apiezon grease to two compression ferrules.
8. Cut a piece of tubing to connect the regulator tee to the Carrier Gas cylinder regulator.
9. Connect the Carrier Gas regulator tee to the Carrier Gas (Nitrogen) supply regulator using
two nuts and ferrules.
10. If there are additional instruments to be connected, repeat steps 2 - 6 and 11 - 13 for each
instrument.
11. Apply a thin coating of Apiezon grease to two compression ferrules.
12. Cut a piece of tubing to connect the regulator tee on the previous instrument to the regulator
tee on the next instrument.
13. Connect the previous Carrier Gas regulator tee to the next Carrier Gas regulator tee using
two nuts and ferrules.
14. Using the plugs removed in step one, cap the open port on the last regulator tee assembly.
Caution: Before proceeding verify that the Carrier Gas and Test Gas inlets on each instrument are
connected to the appropriate gas supply. If the Carrier Gas inlet is connected to the oxygen
supply the Coulox sensor will be damaged and replacement will be required.

OX-TRAN Model 2/22 Operator’s Manual Setting Up
MOCON, Inc. Revision R 2-7
Follow these steps to connect the Test Gas supply lines:
1. Remove the brass plug from the Test Gas fitting on the instrument back panel. Save the
plug as it will be needed later.
2. Cut a piece of tubing approximately 3 inches in length (33 cm).
3. Cut a piece of tubing approximately 13 inches in length (41 cm).
4. Connect the Test Gas fitting on the instrument back panel to the Stainless Steel Filter
(052-084) using the 3 inch length of tubing and two nuts and ferrules. The arrow on the Filter
must point towards the tube connected to the instrument back panel.
5. Connect the input of the Filter to the output fitting on a regulator tee using the 13 inch length
of tubing and two nuts and ferrules.
6. Cut a piece of tubing to connect the regulator tee to the Test Gas cylinder regulator.
7. Connect the Test Gas regulator tee to the Test Gas (Oxygen) supply regulator using two
nuts and ferrules.
8. If there are additional instruments to be connected, repeat steps 2 - 5 and 9 - 10 for each
instrument.
9. Cut a piece of tubing to connect the regulator tees on the previous instrument to the
appropriate regulator tees on the next instrument.
10. Connect the previous Test Gas regulator tee to the next Test Gas regulator tee using two
nuts and ferrules.
11. Using the plugs removed in step one, cap the open port on the last regulator tee assembly.
Caution: Before proceeding verify that the Carrier Gas and Test Gas inlets on each instrument are
connected to the appropriate gas supply. If the Carrier Gas inlet is connected to the oxygen
supply the Coulox sensor will be damaged and replacement will be required.

Setting Up OX-TRAN Model 2/22 Operator’s Manual
2-8 Revision R MOCON, Inc.
Setting the Gas Supply Pressures
Set the Gas Supply Pressures as follows:
1. Set the Carrier Gas cylinder or main line regulator pressure to 35 psi.
2. Set the Test Gas cylinder or main line regulator pressure to 35 psi.
3. Set the pressure on the Carrier Gas regulator-tee so that the gauge reads 25 psi.
4. Set the pressure on the Test Gas regulator-tee so that the gauge reads 25 psi.
Caution: The maximum Carrier Gas pressure to the instrument must not exceed 29 psi.
Input pressures greater than 29 psi (2 bar) will damage the system.
Caution: The maximum Test Gas pressure to the instrument must not exceed 29 psi.
Input pressures greater than 29 psi (2 bar) will damage the system.
Note: When using compressed gas cylinders the tank must be replaced when the pressure falls below 300
psi (on the first stage of the regulator).
System Outgassing
Before the system can be used any air trapped in the gas lines and instrument must be purged out. After
purging the carrier gas supply system, the instrument must be out-gassed. After the initial purge residual
oxygen may still be present in the seals and void spaces within in the instrument. Out-gassing removes this
residual oxygen from the system.
To out-gas the system follow these steps:
1. Verify the Gas Supply Pressures are set correctly; see setting the Gas Supply Pressures on
page 2-8.
2. Turn the instrument on.
3. Mount a film in the Test Cells, for more information see “Mounting a Sample in the Test Cell”
on page 5-6.
4. Load and Clamp the Test Cells, for more information refer to “Loading and Unloading the
Test Cell” on page 5-7.
5. Let the system out-gas for at least one hour before starting a test.