Levenhuk MED PRO 600 Fluo User manual

Levenhuk MED PRO 600 Fluo
Microscope
User Manual
Ръководство за потребителя
Návod k použití
Bedienungsanleitung
Guía del usuario
Használati útmutató
Guida all’utilizzo
Instrukcja obsługi
Manual do usuário
Инструкция по эксплуатации
Kullanım kılavuzu
EN
CZ
ES
IT
RU
BG
DE
HU
PT
PL
TR

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1Trinocular head 15 Knob to adjust halogen lamp position in vertical direction
2Luminous ux switch (splitter) 16 Collector adjustment knob
3Trinocular head lock knob 17 Lamp lament adjustment knob
4Field diaphragm alignment knob 18 Power on/o button
5Field diaphragm opening adjustment knob 19 Fine focusing knob
6Aperture diaphragm opening adjustment knob 20 Knob to move slide in cross direction
7Collector adjustment knob 21 Knob to move slide lengthwise
8Mercury-lled lamp ashlight 22 Condenser
9Cap clamping screw 23 Field diaphragm opening adjustment ring
10 Knob to adjust mercury-lled lamp position in vertical direction 24 Condenser clamping screw
11 Knob to adjust mercury-lled lamp position in horizontal
direction
25 UV protection screen
12 Microscope stand 26 UV protection screen clamping screw
13 Halogen lamp ashlight 27 Switching ring
14 Knob to adjust halogen lamp position in horizontal direction 28 Fluorescent illuminator clamping screw
EN

3
1Тринокулярна глава 15 Бутон за регулиране на положението на халогенната лампа във
вертикална посока
2Превключвател за светлинен поток (разделител) 16 Бутон за регулиране на колектора
3Бутон за заключване на тринокулярната глава 17 Бутон за регулиране на нажежаемата нишка на лампата
4Бутон за регулиране на полевата диафрагма 18 Бутон за вкл./изкл. на захранването
5Бутон за регулиране на отвора на полевата диафрагма 19 Бутон за фино фокусиране
6Бутон за регулиране на отвора на апертурната диафрагма 20 Бутон за напречно преместване на предметното стъкло
7Бутон за регулиране на колектора 21 Бутон за надлъжно преместване на предметното стъкло
8Прожектор с живачна лампа 22 Кондензатор
9Установъчен винт на капачката 23 Пръстен за регулиране на отвора на полевата диафрагма
10 Бутон за регулиране на положението на живачната лампа във
вертикална посока
24 Установъчен винт на кондензатора
11 Бутон за регулиране на положението на живачната лампа в
хоризонтална посока
25 UV предпазител
12 Стойка на микроскопа 26 Установъчен винт на UV предпазителя
13 Прожектор с халогенна лампа 27 Превключващ пръстен
14 Бутон за регулиране на положението на халогенната лампа в
хоризонтална посока
28 Установъчен винт на флуоресцентния осветител
BG
1Trinokulární hlavice 15 Knoík pro nastavení polohy halogenové žárovky ve svislém směru
2Přepínač světelného toku (dělič) 16 Knoík pro nastavení kolektoru
3Pojistný knoík trinokulární hlavice 17 Knoík pro nastavení žhavicího vlákna žárovky
4Knoík pro vyrovnání clony zorného pole 18 Spínač zapnutí/vypnutí
5Knoík pro vyrovnání otevření clony zorného pole 19 Mikrošroub pro jemné zaostření
6Knoík pro vyrovnání otevření aperturní clony 20 Knoík pro posun preparátu příčným směrem
7Knoík pro nastavení kolektoru 21 Knoík pro posun preparátu podélně
8Světlo s rtuťovou výbojkou 22 Kondenzor
9Upínací šroub víčka 23 Prstenec pro vyrovnání otevření clony zorného pole
10 Knoík pro nastavení polohy rtuťové výbojky ve svislém směru 24 Upínací šroub kondenzoru
11 Knoík pro nastavení polohy rtuťové výbojky ve vodorovném
směru
25 Stínítko proti UV záření
12 Rameno mikroskopu 26 Upínací šroub stínítka proti UV záření
13 Světlo s halogenovou žárovkou 27 Přepínací prstenec
14 Knoík pro nastavení polohy halogenové žárovky ve vodorovném
směru
28 Upínací šroub uorescenčního osvětlovacího tělesa
CZ
1Trinokularkopf 15 Drehknopf zur Einstellung der Halogenlampenposition in vertikaler
Richtung
2Lichtstromschalter (Splitter) 16 Kollektor-Einstellknopf
3Feststellknopf für Trinokularkopf 17 Einstellknopf für den Lampenglühfaden
4Ausrichtknopf für die Feldblende 18 Ein-/Ausschalter
5Einstellknopf für die Feldblendenönung 19 Feintrieb
6Einstellknopf für die Önung der Aperturblende 20 Knopf zum Bewegen des Schiebers in Querrichtung
7Kollektor-Einstellknopf 21 Knopf zum Bewegen des Schiebers in Längsrichtung
8Quecksilberdampampe 22 Kondensor
9Kappen-Klemmschraube 23 Einstellring für die Feldmembranönung
10 Drehknopf zur Einstellung der Quecksilberdampampen-Position
in vertikaler Richtung
24 Kondensor-Klemmschraube
11 Drehknopf zur Einstellung der Quecksilberdampampen-Position
in horizontaler Richtung
25 UV-Schutzschild
12 Mikroskopstativ 26 Klemmschraube UV-Schutzschild
13 Halogenlampe 27 Schaltring
14 Drehknopf zur Einstellung der Halogenlampenposition in
horizontaler Richtung
28 Klemmschraube der Leuchtstoampe
DE

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1Cabezal trinocular 15 Perilla para ajustar la posición de la lámpara halógena en
dirección vertical
2Conmutador del ujo luminoso (divisor del haz) 16 Perilla de ajuste de la lente colectora
3Perilla de bloqueo del cabezal trinocular 17 Perilla de ajuste del lamento de la lámpara
4Perilla de alineación del diafragma de campo 18 Botón de encendido / apagado
5Perilla de ajuste del diámetro del diafragma de campo 19 Perilla de enfoque preciso
6Perilla de ajuste del diámetro del diafragma de abertura 20 Perilla para mover el portaobjetos en dirección transversal
7Perilla de ajuste de la lente colectora 21 Perilla para mover el portaobjetos en dirección longitudinal
8Iluminador de lámpara de mercurio 22 Condensador
9Tornillo de sujeción de la tapa 23 Anillo de ajuste de la abertura del diafragma de campo
10 Perilla para ajustar la posición de la lámpara de mercurio en
dirección vertical
24 Tornillo de sujeción del condensador
11 Perilla para ajustar la posición de la lámpara de mercurio en
dirección horizontal
25 Pantalla de protección ultravioleta
12 Soporte del microscopio 26 Tornillo de sujeción de la pantalla de protección ultravioleta
13 Iluminador de lámpara halógena 27 Anillo de conmutación
14 Perilla para ajustar la posición de la lámpara halógena en
dirección horizontal
28 Tornillo de sujeción del iluminador uorescente
ES
1Trinokuláris fej 15 Halogén izzót függőleges irányban állító gomb
2Fényáram kapcsoló (osztó) 16 Kollektor állítógomb
3Trinokuláris fej rögzítőgomb 17 Lámpa izzószál állítógomb
4Világítás rekeszigazító gomb 18 Főkapcsoló gomb
5Világítás rekesznyílás állítógomb 19 Finom élességállító gomb
6Apertúra rekesznyílás szabályozó gomb 20 A tárgylemezt keresztirányban mozgató gomb
7Kollektor állítógomb 21 A tárgylemezt hosszirányban mozgató gomb
8Higanytöltésű izzó lámpa 22 Kondenzor
9Sapka szorítócsavar 23 Világítás rekesznyílás állítógyűrű
10 A higanytöltetű lámpát függőleges irányban állító gomb 24 Kondenzor szorítócsavar
11 A higanytöltetű lámpát vízszintes irányban állító gomb 25 UV-védő szűrő
12 Mikroszkóp állvány 26 UV-védő szűrő szorítócsavar
13 Halogén izzó kisülő lámpa 27 Váltógyűrű
14 Halogén lámpát vízszintes irányban állító gomb 28 Fluoreszcens megvilágítás felfogó csavar
HU
1Testata trinoculare 15 Manopola per regolare la posizione verticale della lampada
alogena
2Deviatore del fascio luminoso (splitter) 16 Manopola di regolazione del collettore
3Manopola di ssaggio della testata trinoculare 17 Manopola di regolazione della luminosità della lampada
4Manopola di allineamento del diaframma di campo 18 Interruttore on/o
5Manopola di regolazione del diaframma di campo 19 Manopola di messa a fuoco ne
6Manopola di regolazione del diaframma di apertura 20 Manopola per spostare il vetrino in direzione trasversale
7Manopola di regolazione del collettore 21 Manopola per spostare il vetrino in direzione longitudinale
8Edicola con lampada a vapori di mercurio 22 Condensatore
9Vite di ssaggio del coperchio 23 Ghiera di regolazione dell’apertura del diaframma di campo
10 Manopola per regolare la posizione verticale della lampada a
vapori di mercurio
24 Vite di ssaggio del condensatore
11 Manopola per regolare la posizione orizzontale della lampada a
vapori di mercurio
25 Schermo protettivo anti UV
12 Stativo del microscopio 26 Vite di ssaggio dello schermo protettivo anti UV
13 Edicola con lampada alogena 27 Ghiera di selezione
14 Manopola per regolare la posizione orizzontale della lampada
alogena
28 Vite di ssaggio dell’illuminatore di uorescenza
IT

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1Głowica trójokularowa 15 Pokrętło do regulacji położenia lampy halogenowej w płaszczyźnie
pionowej
2Przełącznik strumienia świetlnego (rozdzielacz) 16 Pokrętło regulacji kolektora
3Pokrętło blokady głowicy trójokularowej 17 Pokrętło regulacji jasności żarnika lampy
4Pokrętło regulacji przysłony polowej 18 Przycisk wł./wył. zasilania
5Pokrętło regulacji otwarcia przysłony polowej 19 Pokrętło precyzyjnej regulacji ostrości
6Pokrętło regulacji przysłony aperturowej 20 Pokrętło do przesuwania preparatu w kierunku poprzecznym
7Pokrętło regulacji kolektora 21 Pokrętło do przesuwania preparatu w kierunku wzdłużnym
8Oprawa lampy rtęciowej 22 Kondensor
9Śruba mocująca pokrywę 23 Pierścień regulacyjny otwarcia przysłony polowej
10 Pokrętło do regulacji położenia lampy rtęciowej w płaszczyźnie
pionowej
24 Śruba mocująca kondensor
11 Pokrętło do regulacji położenia lampy rtęciowej w płaszczyźnie
poziomej
25 Osłona przed promieniowaniem UV
12 Statyw mikroskopu 26 Śruba mocująca osłonę przed promieniowaniem UV
13 Oprawa lampy halogenowej 27 Pierścień przełączający
14 Pokrętło do regulacji położenia lampy halogenowej w
płaszczyźnie poziomej
28 Śruba mocująca oświetlacz uorescencyjny
PL
1Cabeça trinocular 15 Botão de ajuste da posição da lâmpada de halogéneo na direção
vertical
2Interruptor de uxo luminoso (separador) 16 Botão de ajuste do coletor
3Botão de bloqueio da cabeça trinocular 17 Botão de ajuste do lamento da lâmpada
4Botão de alinhamento do diafragma de campo 18 Botão de ligar/desligar
5Botão de ajuste da abertura do diafragma de campo 19 Botão de focagem na
6Botão de ajuste da abertura do diafragma de abertura 20 Botão para mover a lâmina no sentido transversal
7Botão de ajuste do coletor 21 Botão para mover a lâmina no sentido longitudinal
8Lanterna com lâmpada de mercúrio 22 Condensador
9Parafuso de xação da tampa 23 Anel de ajuste de abertura do diafragma de campo
10 Botão de ajuste da posição da lâmpada de mercúrio na direção
vertical
24 Parafuso de xação do condensador
11 Botão de ajuste da posição da lâmpada de mercúrio na direção
horizontal
25 Painel com proteção UV
12 Braço do microscópio 26 Parafuso de xação do painel com proteção UV
13 Lanterna com lâmpada de halogéneo 27 Anel de comutação
14 Botão de ajuste da posição da lâmpada de halogéneo na direção
horizontal
28 Parafuso de xação do iluminador uorescente
PT

6
1Trinoküler başlık 15 Halojen lambası konumunu dikey yönde ayarlamak için düğme
2Işık akısı anahtarı (bölücü) 16 Kolektör ayar düğmesi
3Trinoküler başlık kilit düğmesi 17 Lamba teli ayar düğmesi
4Alan diyaframı hizalama düğmesi 18 Güç açma/kapama düğmesi
5Alan diyaframı açma ayar düğmesi 19 İnce odaklama düğmesi
6Açıklık diyaframı açma ayar düğmesi 20 Slaytı enlemesine hareket ettirmek için düğme
7Kolektör ayar düğmesi 21 Slaytı boylamasına hareket ettirmek için düğme
8Cıva dolgulu lamba feneri 22 Kondansatör
9Kapak sabitleme vidası 23 Alan diyaframı açma ayar halkası
10 Cıva dolgulu lamba konumunu dikey yönde ayarlamak için düğme 24 Kondansatör sabitleme vidası
11 Cıva dolgulu lamba konumunu yatay yönde ayarlamak için
düğme
25 UV koruyucu eleği
12 Mikroskop sehpası 26 UV koruyucu eleği sabitleme vidası
13 Halojen lamba feneri 27 Değiştirme halkası
14 Halojen lambası konumunu yatay yönde ayarlamak için düğme 28 Floresan aydınlatıcı sabitleme vidası
TR
1Тринокулярная насадка 15 Рукоятка регулировки положения галогенной лампы в верти-
кальном направлении
2Переключатель (делитель) светового потока 16 Рукоятка регулировки коллектора
3Фиксатор тринокулярной насадки 17 Рукоятка регулировки накала лампы
4Рукоятка центрировки полевой диафрагмы 18 Кнопка вкл/выкл питания
5Рукоятка регулировки раскрытия полевой диафрагмы Рукоятка точной фокусировки
6Рукоятка регулировки раскрытия апертурной диафрагмы 20 Рукоятка перемещения препарата в поперечном направлении
7Рукоятка регулировки коллектора 21 Рукоятка перемещения препарата в продольном направлении
8Фонарь ртутной лампы 22 Конденсор
9Винт крепления крышки 23 Кольцо регулировки раскрытия полевой диафрагмы
10 Рукоятка регулировки положения ртутной лампы в
вертикальном направлении
24 Винт крепления конденсора
11 Рукоятка регулировки положения ртутной лампы в
горизонтальном направлении
25 Экран
12 Штатив (стойка) микроскопа 26 Винт крепления экрана
13 Фонарь галогенной лампы 27 Кольцо переключения
14 Рукоятка регулировки положения галогенной лампы в
горизонтальном направлении
28 Винт крепления флуоресцентного осветителя
RU

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1Shutter engagement knob 9Clamp
2Field diaphragm alignment knob 10 Stage
3Vertical tube 11 Decorative base
4Diopter adjustment ring 12 Fine focusing knob
5Eyepieces 13 Coarse focusing knob
6Corrugated revolver switching ring 14 Ring to adjust coarse focusing ease of movement
7Objective lens 15 Fluorescent illuminator
8Slide holder
EN

8
1Zár működtető gomb 9Bilincs
2Világítás rekeszigazító gomb 10 Tárgyasztal
3Függőleges tubus 11 Díszes talpazat
4Dioptria szerkezet gyűrűje 12 Finom élességállító gomb
5Szemlencsék 13 Durva élességállító gomb
6Rovátkolt revolver váltógyűrű 14 Gyűrű a durva élességállító mozgás szabályozására
7Objektívlencse 15 Fluoreszcens megvilágítás
8Tárgylemez leszorító
HU
1Бутон за включване на затвора 9Скоба
2Бутон за регулиране на полевата диафрагма 10 Предметна маса
3Вертикална тръба 11 Декоративна основа
4Пръстен за диоптричния механизъм 12 Бутон за фино фокусиране
5Окуляри 13 Бутон за грубо фокусиране
6Рифелован превключващ пръстен на револверната глава 14 Пръстен за регулиране на лекотата на движение за грубо
фокусиране
7Обектив 15 Флуоресцентен осветител
8Държач за образец
BG
1Knoík spouště závěrky 9 Svorka
2Knoík pro vyrovnání clony zorného pole 10 Pracovní stolek
3Svislý tubus 11 Dekorativní základna
4Prstenec mechanismu dioptru 12 Mikrošroub pro jemné zaostření
5Okuláry 13 Makrošroub pro hrubé zaostření
6Zvlněný revolverový přepínací prstenec 14 Prstenec k nastavení snadnosti pohybu hrubého zaostření
7Objektiv 15 Fluorescenční osvětlovací těleso
8Držák preparátů
CZ
1Auslöser-Feststellknopf 9Klemme
2Ausrichtknopf für die Feldblende 10 Objekttisch
3Vertikaler Tubus 11 Dekorativer Sockel
4Ring des Dioptrienmechanismus 12 Feintrieb
5 Okulare 13 Grobtrieb
6Gerändelter Objektivwechselring 14 Ring zur Einstellung der Leichtgängigkeit des Grobtriebs
7Objektivlinse 15 Leuchtstoampe
8Objektträger
DE
1Perilla de activación del obturador 9Abrazadera
2Perilla de alineación del diafragma de campo 10 Platina
3Tubo vertical 11 Base decorativa
4Anillo de ajuste de las dioptrías 12 Perilla de enfoque preciso
5Oculares 13 Perilla de enfoque aproximado
6Anillo estriado de rotación del revólver 14 Anillo de ajuste del movimiento del enfoque aproximado
7Lente objetivo 15 Iluminador uorescente
8Soporte para muestras
ES

9
1Manopola di azionamento dell’otturatore 9Morsetto
2Manopola di allineamento del diaframma di campo 10 Tavolino portaoggetti
3Tubo verticale 11 Base di copertura
4Ghiera di regolazione diottrica 12 Manopola di messa a fuoco ne
5Oculari 13 Manopola di messa a fuoco grossolana
6Ghiera di selezione del revolver a lobi 14 Ghiera per regolare la scorrevolezza del movimento di messa a
fuoco grossolana
7Lente obiettivo 15 Illuminatore di uorescenza
8Molletta ferma vetrini
IT
1Pokrętło aktywacji przesłony 9Zacisk
2Pokrętło regulacji przysłony polowej 10 Stolik
3Tubus pionowy 11 Osłona maskująca
4Pierścień mechanizmu regulacji dioptrii 12 Pokrętło precyzyjnej regulacji ostrości
5Okulary 13 Pokrętło zgrubnej regulacji ostrości
6Karbowany pierścień przełączający głowicy obrotowej 14 Pierścień do regulacji łatwości poruszania stolikiem podczas
regulacji zgrubnej
7Obiektyw 15 Oświetlacz uorescencyjny
8Uchwyt na preparaty
PL
1Botão de acoplamento do obturador 9Pinça
2Botão de alinhamento do diafragma de campo 10 Platina
3Tubo vertical 11 Base decorativa
4Anel de mecanismo de dioptria 12 Botão de focagem na
5Oculares 13 Botão de focagem grosseira
6Anel de comutação do revólver ondulado 14 Anel de ajuste da facilidade de movimento da focagem grosseira
7Lente da objetiva 15 Iluminador uorescente
8Suporte de lâminas
PT
1Рукоятка включения шторки 9Прижим
2Рукоятка центрировки полевой диафрагмы 10 Предметный столик
3Вертикальный тубус 11 Декоративное основание
4Кольцо диоптрийного механизма 12 Рукоятка точной фокусировки
5Окуляры 13 Ручка грубой фокусировки
6Рифленое кольцо переключения револьвера 14 Кольцо регулировки плавности хода грубой фокусировки
7Объектив 15 Флуоресцентный осветитель
8Препаратодержатель
RU
1Obtüratör etkinleştirme düğmesi 9Kelepçe
2Alan diyaframı hizalama düğmesi 10 Lamel yuvası
3Dikey tüp 11 Dekoratif taban
4Diyopter mekanizması halkası 12 İnce odaklama düğmesi
5Göz mercekleri 13 Kaba odaklama düğmesi
6Kıvrımlı döner geçiş halkası 14 Kaba odaklama hareket kolaylığını ayarlama halkası
7Objektif merceği 15 Floresan aydınlatıcı
8Slayt tutucu
TR

10
EN Levenhuk MED PRO 600 Fluo Microscope
Microscope description and operation
Application
The microscope is designed for diagnostic testing, including by immunouorescence method, in clinical, microbiological,
pathoanatomical, and other laboratories in medical institutions. Besides, it may also be used in veterinary science, crop
growing, bioengineering, pharmaceutical industry, for expertise in the sphere of criminalistics, state epidemiological
surveillance, environmental protection. The microscope is used to study stained and unstained slides in the form of smears and
microsections in transmitted light.
In the luminescence light, the microscope makes it possible to detect dangerous bacterial and virus infections when observing
objects stained by Auramine, Acridine orange, FITC etc.
Being properly operated, the microscope is safe for health, life, property of the consumer and for the environment. The
microscope stand is of anti-vibration design. The microscope is made to operate at ambient air temperature from +10 to +35°С
(50 to 95°F) and relative humidity of 80% max. The oil-immersion objective lens shall be operated indoors at ambient air
temperature from +15 to +25°С (59 to 77°F).
Microscope design and operation principle
TO PREVENT MICROSCOPE BREAKDOWN, BEFORE STUDIES, CAREFULLY REVIEW THE RULES OF HANDLING AND
PROCEDURE OF WORKING WITH THE MICROSCOPE SPECIFIED IN THIS OPERATION MANUAL.
Fluorescent microscope operating principle is based on using a phenomenon of uorescence (luminescence) of observed objects
caused by rays of light having a specic spectrum. To excite uorescence, objects are illuminated from the top through the
objective lens, with a mercury-led lamp used as a source of such light. The luminous ux required to excite uorescence is
separated from the total radiation of the mercury-lled lamp with lters, conventionally referred to as the excitation lters.
To guide the luminous ux to the objective, a beam splitter with a special interference coating is used that mostly reects
excitation light and transmits object uorescence light. The excitation lter, the beam splitter and the cut-o lter (used
to absorb the residual excitation radiation) are combined in a single beam splitting unit. A kit of ve beam splitting units is
mounted on a turret having a free socket for operating in transmitted light.
The optic system enabling object study in uorescence light is made in the form of a detachable illuminator installed on the
microscope stand. The microscope stand enables observation of objects illuminated with transmitted light.
Description and operation of components
Microscope stand
The microscope stand (g. 1, 12) has ergonomic and stable shape, and is made of metal.
There is a double-stage focusing mechanism for vertical movement of the bracket (g. 2, 3) with a coordinate stage (g. 2, 10)
and a revolving nosepiece for objective lens xation (g. 2, 7) on the stand. A uorescent illuminator (g. 2, 15) is installed on
top of the stand and is xed with a screw (g. 1, 28). The microscope stand base contains the transmitted light illumination
system and halogen lamp power source of 12V/30W. There is a socket for connection of a power cable on the rear surface of the
base on the left.
The power source is built into the microscope stand base. Power on/o button (g. 1, 18) energizes the halogen lamp installed
in the ashlight (g. 1, 13). Power is o in "O" position. Halogen lamp lament is adjusted by a knob (g. 1, 17).
There is an iris eld diaphragm, whose opening is adjusted by a ring (g. 1, 23), located on the upper surface of the microscope
base under the condenser (g. 1, 22). A decorative base (g. 2, 11) is put on the microscope stand base.
Revolving nosepiece
A six-position revolving nosepiece provides for objective lens (g. 2, 7) setting into working parfocal position. The revolving
nosepiece is inclined towards the microscope stand to provide space for installation and replacement of the examined slides.
Objective lenses are replaced by rotation of a corrugated ring (g. 1, 27) of the revolving nosepiece to the xed position.
Focusing mechanism
The focusing mechanism is designed for vertical displacement of the stage (g. 2, 10) when the microscope is focused for sharp
object image. The range of stage movement along height is 25mm. Vertical displacement of the stage is performed by coaxial
knobs (g. 2, 12 and 13) located on the left side of the microscope stand. The ne focusing mechanism knob (g. 2, 12) has a
scale with division value of 2µm. Behind the knob (g. 2, 13) there is a ring (g. 2, 14), designed to adjust ease of movement
during coarse focusing. There is a ne focusing mechanism knob (g. 1, 19) on the right side of the stand (g. 1, 12).
Stage
A stage (g. 2, 10) is equipped with a mechanism of coordinate object displacement in the horizontal plane in two mutually
perpendicular directions. Stage and slide holder design (g. 2, 8) provides for the possibility to install two slides and move them
by 85mm in cross direction and 50mm lengthwise. Displacement is controlled by low-set coaxial knobs from the right side of the
stand. With the help of the knob, the object is moved in the cross direction (g. 1, 20) and lengthwise (g. 1, 21). The division
value is 1mm, the nonius division value is 0.1mm. The object is xed on the stage surface between the holder and the clamp
(g. 2, 9) of the slide holder (g. 2, 8). To install the object, the clamp (g. 2, 9) is taken aside. The stage surface has a solid
coating resistant to disinfection and wear. Stage dimensions are 180x160mm.

11
Transmitted light illumination system
The illumination system of the microscope is critical to obtain a contrast and evenly illuminated image of the objects under
the microscope. The illumination system built into the microscope stand base (g. 1, 12) is arranged according to the Köhler’s
principle in its classic version. The halogen lamp ashlight is installed on the rear wall of the base and is xed with a screw
(g. 4, 2). The iris eld diaphragm node is located on the stand base under the condenser (g. 1, 22), the diaphragm opening is
adjusted with a ring (g. 1, 23).
The condenser (g. 1, 22) is used to focus the eld diaphragm image in the slide plane.
The illuminator is turned on with the help of a switch (g. 1, 18) in position "I". The lamp brightness may be varied by rotation of
the lamp lament adjustment knob (g. 1, 17). The lamp is energized through a power supply source built into the microscope
stand base.
The halogen lamp installation in the ashlight is shown in g. 4. To access the lamp holder, it is necessary to unscrew the
screw (g. 1, 28) and to extend the cover (g. 4, 5). To install the cover (g. 4, 5) on the ashlight, it is necessary to take
xators (g. 4, 6) behind the ashlight casing.
Bright-eld condenser
An Abbe condenser (g. 1, 22) is included in the microscope package for operation in the bright eld. The condenser is installed
into a bracket (g. 3, 3) under the microscope stage and is xed with a screw (g. 1, 24).
An iris aperture diaphragm, diameter of which is adjusted with a knob (g. 3, 1) changes aperture of the cone of rays
illuminating the slide. A scale is applied onto the condenser frame, which makes it possible to reproduce illumination conditions
selected for every objective lens — knob positions (g. 3, 1).
There is an option to exclude the condenser frontal objective lens from the path of rays with the knob (g. 3, 4) when working
with low magnication objective lenses. Screws (g. 3, 2) serve to align the eld diaphragm image by displacement of the
condenser in the horizontal plane. Condenser displacement along the optical axis of the microscope when focusing the eld
diaphragm image is carried out with the knob (g. 3, 5).
1Condenser aperture diaphragm opening
adjustment knob
2Condenser alignment screws
3Condenser bracket
4Condenser frontal objective lens switching
knob
5Condenser vertical displacement knob
Fig. 3: Condenser
1Cap clamping seat
2Flashlight clamping knob
3Halogen lamp holder
4Halogen lamp
5Flashlight detachable cap
6Fixators
7Cap clamping screw
Fig. 4: Halogen lamp ashlight

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Impinging light illumination system
The impinging light illumination system is made in the form of a detachable module — a uorescent illuminator (g. 2, 15). The
module is installed with a lower ange into the microscope stand seat and is xed with a screw (g. 4, 7). A mercury-lled lamp
ashlight is xed on the illuminator (g. 1, 8).
The uorescent illuminator (g. 2, 15) comprises a six-seat turret with 5 spectral beam splitting units and a free seat for
transmitted light. Seats are numbered and provided with captions that contain information about spectral characteristics of
lters and a dichroic mirror (a beam splitter) given in table.
Excitation lter Dichroic mirror Cut-o lter Unit designation
No.1 Free seat
No.2 510—560 575 590 G
No.3 450—490 505 520 B
No.4 400—440 455 470 BV
No.5 380—420 430 450 V
No.6 330—380 400 420 U
Marking of beam splitting units complies with color of beam of rays exciting uorescence of examined objects.
For example, when a carriage is in "G" position, a green spectral region of 510—560nm is identied from the total radiation ux
of the mercury-lled lamp, and when it is in "B" position — a spectral region of 450—490nm (blue) is identied.
The illuminator comprises eld and aperture diaphragms (FD and AD). The eld diaphragm is equipped with an alignment
device (g. 1, 4), the position adjustment knobs are located on the illuminator casing on the right and left. The knob (g. 1, 5)
adjusts eld diaphragm opening, the knob (g. 1, 6) adjusts aperture diaphragm opening. To change the diaphragm dimensions,
knobs (g. 1, 5 and 6) are pushed into the casing. A plug to intercept light that is controlled by a knob is installed closer to the
ashlight in the illuminator casing.
Mercury-lled lamp ashlight
The mercury-lled lamp ashlight (g. 1, 8) is xed on the illuminator with a bayonet ring as the adjusting ring (g. 5, 7) and
the xator (g. 5, 8) are brought closer to the illuminator end.
WARNING! PRIOR TO REMOVAL OF THE FLASHLIGHT FROM THE HEAD CASING, IT IS NECESSARY TO DISCONNECT THE
MERCURY-FILLED LAMP POWER SUPPLY UNIT FROM THE GRID!
The mercury-lled lamp is aligned by knobs (g. 1, 10 and 11). Knob 10 serves to move the holder with the lamp in vertical
direction, and knob 11 — to move it in horizontal direction. The ashlight detachable cap (g. 5, 1) is xed with a screw (g. 1, 9),
on the inner side of which there is a mercury-lled lamp holder. The mercury-lled lamp (g. 5, 4) is installed into bushings (g. 5,
2 and 5) and is xed by screws (g. 5, 3 and 6).
WARNING! FOR MICROSCOPE TRANSPORTATION, REMOVE THE MERCURY-FILLED LAMP FROM THE FLASHLIGHT.
Inside the ashlight, there is a collector projecting the mercury-lled lamp discharge arc image into the exit pupil of the
objective installed in the path of rays. Knob 7 (g. 1) adjusts collector position along the illuminator axis.
1Mercury-lled lamp ashlight
detachable cap
2Bushing
3Screw
4Mercury-lled lamp
5Bushing
6Screw
7Adjusting ring
8Fixator
Fig. 5: Mercury-lled lamp ashlight

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1Amperemeter
2Lamp ignition button
3Mercury-lled lamp operation-
time counter
4Power on/o button
00939
Fig. 6: Mercury-lled lamp power supply unit
Trinocular head
The trinocular head (g. 1, 1) is designed to work with objective lenses, the optical length of tubes in which is "innity". The
head enables binocular observation with eyepieces (g. 2, 5) and image output through a vertical tube (g. 2, 3) for xation.
The head enables positioning of eyepiece tubes in accordance with the required eyepiece spacing (observer’s eye base). The
distance between axes of eyepieces (g. 2, 5) is adjusted by rotation of eyepiece tubes in the range from 50 to 75mm. The
ring (g. 2, 4) in the left eyepiece tube performs dioptric adjustment of eyepiece position (g. 2, 5) for ±5 diopters.
Luminous ux is directed into a vertical tube by switching the knob (g. 1, 2). The knob can be switched into three positions,
providing for three options of beam splitting: observation only, observation and documentation and only documentation. The
head is installed into the seat of the uorescent illuminator and is xed by the xator (g. 1, 3).
Objective lenses
All objective lenses (g. 2, 7) included into the scope of supply are designed for innite optical length of the tube and have
semi-apochromatic correction. Parfocal height of objective lenses is 45mm.
Linear magnication and numerical aperture are engraved on the casing of each objective lens. There is also a color marking
compliant with magnication and information on a cover slip. The package includes objective lenses for working with the slides
protected by cover slips and also objective lenses not requiring a slide to be protected by a cover slip.
Specications of objective lenses
Correction type Linear magnication
and numerical aperture System
Linear eld in space of
objects
with eyepiece 10х/22, µm
Total microscope
magnication with 10x
eyepiece, x
Plan uorite
(semi-apochromatic) 4х/0.15 Dry 550 40
Plan uorite
(semi-apochromatic) 10х/0.35 Dry 220 100
Plan uorite
(semi-apochromatic) 20х/0.60 Dry 110 200
Plan uorite
(semi-apochromatic) 40х/0.75 Dry 55 400
Plan uorite
(semi-apochromatic) 100х/0.90 * Dry 22 1000
Plan uorite
(semi-apochromatic) 100х/1.25 * Oil 22 1000
* Not included into standard package
"∞/−" inscription on the objective lens means that the objective lens may handle slides both with a cover slip or without it.
40x and 100x magnication objective lenses are equipped with elastic frames preventing objects and frontal objective lenses
against damage when focusing on object surface.
WARNING! IF OBJECTIVE LENSES ARE DAMAGED, THEY SHOULD BE REPAIRED IN THE MANUFACTURER’S SERVICE
CENTER.
Eyepieces
The microscope package comprises two wide-eld eyepieces with 10x magnication and 22mm linear eld in the image plane.
Microscope use
Operation limits
The microscope should be used in premises where pushes and vibrations are hardly sensed, with no sources of intensive external
exposure, i.e. sources of electromagnet radiation. There shall be no excessive dust, acid, alkali vapors and other chemically
active substances in the premises. The microscope should not be operated in brightly lit premises.
The microscope is designed for operation under the conditions of moderate and cold climate in laboratory premises at air

14
temperature from +10 to +35°С (50 to 95°F) and upper value of relative air humidity of 80% max.
Microscope unpacking
Unpack the microscope carefully and install it on a smooth surface. Check the microscope package contents. Visually inspect all
elements included in the scope of supply, identify their purpose, make sure there are no damages and start assembling.
Microscope preparation for operation
Installation of modular units
• Install the decorative base on the microscope stand base (supply with the installed decorative base is possible).
• Install the halogen lamp ashlight (g. 1, 13) on the microscope stand base and x with a clamping knob (g. 4, 2).
• Install a uorescent illuminator (g. 2, 17) on the microscope stand ange (g. 1, 12). When installing the illuminator, rst
press the conical surface of the slip on ange to two supports arranged on the right in the stand seat, then clamp the ange
with a screw (g. 1, 28).
• Install the knob (g. 2, 1) in the position of beam of rays interception with a shutter, having extended it from the casing.
• Place the mercury-lled lamp ashlight (g. 1, 8) on a table, unscrew the cap clamping screw (g. 1, 9) and remove the cap
(g. 5, 1).
• Take the mercury-lled lamp from the microscope package, install it into bushings (g. 5, 2 and 5) on the cap (g. 5, 1) and
x with screws (g. 5, 3 and 6).
WARNING! DO NOT TOUCH THE MERCURY-FILLED LAMP BULB! AFTER LAMP INSTALLATION, DEGREASE THE BULB
SURFACE WITH ALCOHOL SOLUTION.
• Install the cap (g. 5, 1) into the mercury-lled lamp ashlight (g. 1, 8) and x with a screw (g. 1, 9).
• Install the mercury-lled lamp ashlight (g. 1, 8) on the uorescent illuminator (g. 2, 15), using the xator and the
adjusting ring (g. 5, 7 and 8), x with a bayonet ring located on the illuminator.
• Connect the cable from the ashlight to the slot on the rear surface of the mercury-lled lamp power supply unit (g. 6).
• Connect the power cord to the power socket on the rear surface of the mercury-lled lamp power supply unit. Make sure
that the switch is in "O" position (g. 1, 18).
• Install the trinocular head (g. 1, 1) on the uorescent illuminator ange (g. 2, 15), x with a lock knob (g. 1, 3). Install
the luminous ux switch (splitter) (g. 1, 2) into "Observation only" position.
• Install eyepieces (g. 2, 5) into eyepiece tubes.
• Install the beam splitting units switching ring (g, 1, 27) to position No.1.
• Put the stage (g. 2, 10) down by rotation of the coarse focusing mechanism knob (g. 2, 13) until stop.
• Install objective lenses (g. 2, 7) into revolving nosepiece seats in the ascending order of their magnications.
• Turn the lamp lament adjustment knob (g. 1, 17) in the direction of brightness reduction until stop.
• The switch (g. 1, 18) should be installed into "O" position.
• Connect the power cord to the power socket on the rear surface of the stand base (g. 1, 12).
• Install the UV protection screen (g. 1, 25) and x it with screws (g. 1, 26).
Microscope use
Safety precautions
The microscope may be handled by persons with special medical education. The source of hazard in microscope operation is
electric current. Microscope design prevents accidental contact with energized current-conducting parts.
WARNING! REPLACE LAMPS IN FLASHLIGHTS WHEN THE MICROSCOPE AND THE MERCURY-FILLED LAMP POWER
SUPPLY UNIT ARE DISCONNECTED FROM THE GRID. TO AVOID HAND SKIN BURN FROM THE LAMP BULB, REPLACE
THE LAMP 15—20 MINUTES AFTER DISCONNECTION.
When safety fuses are replaced, it is necessary to install new safety fuses with the same ratings as before.
After the end of operation, the microscope and the mercury-lled lamp power supply unit must be disconnected from the grid.
It is not recommended to leave the appliances connected to the grid unattended.
Perform repair and preventive maintenance only after disconnection of the appliances from the grid.
Observation of objects in transmitted light
Halogen lamp activation and illumination setup
Connect the microscope power cord to AC grid.
Activate the halogen lamp, having set the switch (g. 1, 18) in "I" position.
Adjust lamp brightness by rotation of the lament adjustment knob (g. 1, 17).
Image quality in the microscope to a large extent depends on illumination, therefore, illumination setup is an important
preparatory operation, which must be performed as follows:
• put the object on the stage (g. 2, 10) of the microscope;
• activate the objective lens with 4x or 10x magnication into the path of rays (it is recommended to start the process of
focusing from low or medium magnication objective lenses with suciently large elds and operating distances);
• focus the microscope by rotation of knobs (g. 1, 14 and 15);
• cover the eld diaphragm with a ring (g. 1, 23), and the aperture diaphragm of the condenser — with a knob (g. 3, 1);

15
• observing the object image, focus the condenser, moving it along the height with the knob (g. 3, 5) for sharp image of
the iris eld diaphragm;
• if the eld diaphragm image is displaced, bring the image into the eld center with condenser alignment screws (g. 3, 2);
• open the eld diaphragm with a ring (g. 1, 23) along the eyepiece eld diameter — so that edges of the iris diaphragm
are slightly beyond the eyepiece eld;
• remove the eyepiece from the right eyepiece tube;
• observing the exit pupil image in the right tube, open the aperture diaphragm of the condenser with the knob (g. 3, 1)
to the exit pupil size. Make sure that the lamp lament image lls the eye. If the image is displaced by the lamp position
adjustment knobs (g. 1, 14 and 15), align the lament image. Using the collector adjustment knob (g. 1, 16), ll the
objective exit pupil with light;
• install the eyepiece in the right eyepiece tube.
Normal operation of the illumination system is provided only when slides with thickness of 1—1.2mm are used.
Microscope focusing for binocular observation
Focus the microscope on the object when observing through a binocular tube as follows:
• put the object on the stage (g. 2, 10) of the microscope;
• put the objective lens with the necessary magnication into the path of rays;
• rotating the coarse focusing knob (g. 2, 13), carefully lift the stage to the distance of 0.5mm to the objective lens;
• observing with your right eye into the eyepiece installed into the right eyepiece tube, slowly put the stage down,
rotating the coarse focusing knob (g. 2, 13). As the object contours appear, focus the microscope using a ne focusing
knob (g. 1, 19 or g. 2, 12);
• observing with your left eye (the right eye is closed) into the eyepiece installed in the left eyepiece tube, get the sharp
image of the object by rotation of the diopter mechanism ring (g. 2, 4). Do not touch the focusing mechanism knobs
when doing this;
• set the distance between axes of eyepiece tubes of the binocular head in accordance with the observer’s eye base
by rotation of the casings with eyepiece tubes in respect to the hinged joint axis so that images of the object in each
eyepiece of the head when observing with two eyes are perceived by the observer as one;
• start investigating the slide.
To achieve the best image quality, it is recommended to close the aperture diaphragm of the condenser by 1/3 of the
objective exit pupil for each objective lens.
Selection of objective lenses
It is recommended to investigate the object from the objective lens of the lowest magnication, which is used as a search
objective lens when selecting a site for more detailed investigation.
After the site for investigation was selected, put its image into the microscope eld center. If this operation is performed
not carefully enough, the object site of interest for the observer may not get into the eld of the stronger objective lens as
magnications are changed.
Then you can go to work with stronger objective lenses, including an objective lens for oil immersion.
Immersion objective lens handling
Handle the immersion objective lens in the premises with temperature from +15 to +25°С (59 to 77°F). Use immersion oil
with refraction index nD = 1.515.
WARNING! DO NOT USE SUBSTITUTES INSTEAD OF IMMERSION OIL AS IT MAY SIGNIFICANTLY WORSEN IMAGE
QUALITY.
Prior to immersion objective lens handling, set up the microscope as specied in subsections "Halogen lamp activation and
illumination setup" and "Selection of objective lenses" and clearly identify the object site for more detailed investigation.
To handle the immersion objective lens:
• lower the stage with a knob (g. 2, 13);
• apply immersion oil on the object;
• carefully lift the stage using coarse focusing knobs (g. 2, 13) until the objective lens contacts with the immersion drop
on the object;
• observing into the eyepieces and using the ne focusing knob (g. 1, 19 or g. 2, 12), get the sharp image of the
examined object.
If images of air bubbles that may be contained in the immersion oil layer appear in the eyepiece eld in process of focusing,
use coarse focusing knobs (g. 2, 13), put the stage down and repeat focusing.
To investigate the objects, it is necessary to make sure that the iris diaphragm of the objective lens 100х/1.3 is open.
To increase the image contrast, rst adjust the condenser aperture diaphragm with a knob (g. 3, 1), then perform ner
adjustment of the contrast with the objective lens iris diaphragm.
Upon completion of operation, remove immersion oil from the frontal objective lens with blotting paper and wipe the
contaminated surfaces with cotton wrapped on a stick and slightly soaked in ether or alcohol mix.
Do not push on the frontal objective lens when cleaning.
If the image contrast has lessened or sharpness disappeared as a result of wrong handling of the immersion objective lens,
the following is recommended:
• unscrew the objective lens, clean the frontal lens as shown above;
• using oblique light from a table-lamp and a magnifying glass, make sure that there is no dirt, traces of immersion oil, cracks
or dents on the frontal lens surface;

16
• check the microscope illumination setup (the condenser aperture diaphragm shall be open by size of the lens eye or by 2/3
of the eye size).
Observation of objects in uorescence light
Mercury-lled lamp activation and illumination setup
Connect the mercury-lled lamp power supply unit to the grid. Activate the mercury-lled lamp by putting the power switch in "I"
position.
It takes at least 10 minutes for the mercury-lled lamp to reach operating parameters. Normal mode of lamp operation means that
amperemeter and voltmeter arrows are in the middle of the scale.
WARNING! DO NOT DEACTIVATE THE MERCURY-FILLED LAMP EARLIER THAN 15 MINUTES AFTER IGNITION! YOU CAN
REACTIVATE THE LAMP ONLY 15—20 MINUTES AFTER ITS DEACTIVATION!
Draw "+" sign on a sheet of white paper sized as the stage and put the sheet on the stage. Put the 4x magnication objective lens
into the path of rays. Slide the knob (g. 2, 1) into the casing and put the lter in the path of rays. Using knobs (g. 1, 5 and 6),
open the eld and aperture diaphragm. Install the ring (g. 1, 27) to switch spectral beam splitting units in position No.3 ("B").
Observing into the eyepiece and moving the stage with the coarse focusing knob (g. 2, 13), get the image of the paper sheet
surface. Moving the paper sheet on the stage surface, bring the "+" image into the eyepiece eld center. Put the revolver seat
free of the objective lens into the path of rays.
Observing from the side (not into the eyepiece) on the paper sheet surface, move the collector with the knob (g. 1, 7), to get
the sharpest image of the mercury-lled lamp discharge arc and its electrodes. Using knobs 10 and 11 (g. 1), which regulate
the mercury-lled lamp position, put the discharge arc image onto "+" sign on the paper sheet surface (in the eyepiece eld
center). Put the 4x and then 10x magnication objective lens into the path of rays. Observing into the eyepiece and moving the
collector with the knob (g. 1, 7), achieve the most even illumination of the eld.
Observation of objects
For investigation in the uorescence light, the objects are exposed to treatment with special dyes (uorochromes) having
specic spectral characteristics of absorption (excitation) and glow. In accordance with the uorochrome used to treat the slide,
it is necessary to install one of ve beam splitting units into the path of rays of the uorescent illuminator given in table 1 of
subsection "Impinging light illumination system".
For example, for rather common treatment of slides using FITC, spectral unit No.3 ("B") is required, for Auramine — unit No.4
("V"), for stains glowing in the red spectral range, — unit No.2 ("G"). For DAPI and Hoechst stains, unit No.6 ("U") is used; when
working with this unit, the lter must be removed from the path of rays with a knob (g. 2, 1).
Further do the following:
• install the object on the stage (g. 2, 10) of the microscope;
• activate the objective lens with 10x magnication into the path of rays (it is recommended to start the process of focusing
from low or medium magnication objective lenses with suciently large elds and operating distances);
• focus the microscope by rotation of knobs (g. 2, 12 and 13) for the sharp image of the object;
• cover the eld and aperture diaphragm with knobs (g. 1, 5 and 6);
• observing the object image, make sure that the image of the iris eld diaphragm is located concentrically to the eyepiece
eld (if the diaphragm is displaced, align it);
• if the eld diaphragm image is displaced, put the image in the center of the eld using knobs (g. 2, 2);
• open the eld diaphragm with a knob (g. 1, 5) along the eyepiece eld diameter so that edges of the iris diaphragm are
slightly beyond the eyepiece eld;
• open the aperture diaphragm with a knob (g. 1, 6), observing the eyepiece eld, make sure that the illumination is quite
even, if necessary, adjust the collector focusing with a knob (g. 1, 7);
• perform focusing on the object for observation with binocular tubes in the same manner as shown in subsection "Microscope
focusing for binocular observation" when working in transmitted light;
• start investigating the objects with short breaks in your work. To prevent slide fading, it is necessary to intercept the
luminous ux from the lamp with a knob (g. 2, 1).
Microscope magnication and eld diameter on the object
The total microscope magnication Г in process of visual observation with a binocular head is determined using the following
formula:
Γ= βob ∙ βh ∙ Γeye
where βob — linear magnication of the microscope objective lens;
βh — linear magnication of the head equal to 1.0;
Гeye — visible magnication of the eyepiece.
The eld diameter observed on the object, Dob mm, is determined using the following formula:
Dob = Deye
βob ∙ βh
where Deye — diameter of the eyepiece eld limited by the eld diaphragm of the eyepiece, in mm.

17
Possible faults of the microscope and methods of their elimination
External manifestation of
the fault Probable cause Method of elimination
Cut or uneven illumination
The revolving nosepiece is not in xation
position (the objective lens is not on the
microscope axis)
Tighten the revolving nosepiece and put the
objective lens into the xed position, i.e. on
the optical axis
Any objective or eyepiece lens etc. is
contaminated Visually inspect lenses and clean them
The condenser is not in operating position —
too low or warped Put the condenser in the operating position
There is dust, dirt in the
eld Any lens or the stage is contaminated Remove dirt
Poor quality of object
image (low resolution, poor
contrast)
There is no cover slip on the object, or its
thickness does not comply with the standard
Use the object with a cover slip of standard
thickness of 0.17mm
The object is put with its cover slip down Turn the object over
Immersion oil got onto the frontal objective
lens. There is dry immersion oil on frontal
objective lens 100х ∞/0.17
Clean the immersion oil from the surfaces of
frontal objective lenses
Immersion oil was not applied on the 100x
frontal objective lens Apply oil
There are bubbles in the immersion oil Clean the immersion oil from the objective
lens, the object, the stage and reapply it
The aperture diaphragm of the condenser is
open or closed too much Set the necessary diaphragm size
Object images when
observed with two eyes in
two eyepieces do not match
Eyepiece tubes of the binocular head are not
set in accordance with the observer’s eye base
Install the binocular head in accordance with
the instructions of subsection "Microscope
focusing for binocular observation"
When switching the low
magnication objective
lens to higher magnication
objective lens, the
objective lens hits the
object
The stage with the object is turned over Install the object with the stage up
The cover slip is too thick Use the cover slip of standard thickness
The halogen lamp is not on
after activation
The lamp is out.
The fuse blew out (safety fuse).
Replace the lamp in accordance with the
instructions of subsection "Transmitted light
illumination system"
Disconnect the microscope from the grid and
replace the fuses
The mercury-lled lamp
does not go on or went o
The power supply unit is o
Check POWER indication on the power supply
unit casing; if it is missing, disconnect from
the grid and replace safety fuses with the new
ones from the package
The mercury-lled lamp is installed wrongly
Disconnect the power supply unit from the
grid, disconnect the ashlight cord from the
unit.
Remove the ashlight (after it cooled down),
check the proper installation of the lamp
according to the instructions of subsection
"Impinging light illumination system"
The object uorescence
brightness has reduced
greatly
The lamp failed — the bulb has become blurred Replace the lamp following the instructions of
subsection "Impinging light illumination system"

18
Specications
Microscope type biological/optical
Research method uorescence, bright eld
Magnication, x 40—400
Optics material optical glass
Eyepiece head trinocular, inclined at 30°, with luminous ux switching (splitting)
Interpupillary distance, mm 50—75
Eyepiece tube diameter, mm 30
Third vertical eyepiece tube, mm 23.2
Eyepieces wide eld WF 10x/22mm with eyecups (2pcs.)
Objective lenses innite semi-apochromatic luminescent (uorescent), objective lenses: 4х, 10х, 20х, 40х
Revolving nosepiece 6 objectives
Stage mechanical double-layer, 180x160mm, with a mechanical scale
Stage moving range, mm 85х50
Condenser detachable Abbe condenser N.A. 1.25 with an iris diaphragm and a lter holder
Diaphragm iris, eld
Focusing coaxial, coarse and ne focusing; ne focusing scale: 0.002mm
Body material metal
Illumination lower (12V/30W halogen lamp) with brightness adjustment
Fluorescent module
lters "G", "B", "BV", "V", "U";
mercury-lled lamp (100W) with external power supply unit;
UV protection screen
Power supply AC adapter 100—220V/50—60Hz
Levenhuk reserves the right to make changes to the product range and specications without prior notice.
CAUTION! PLEASE REMEMBER THAT MAINS VOLTAGE IN MOST EUROPEAN COUNTRIES IS 220—240V. IF YOU WANT TO
USE YOUR DEVICE IN A COUNTRY WITH A DIFFERENT MAINS VOLTAGE STANDARD, REMEMBER THAT USE OF A
CONVERTER IS ABSOLUTELY NECESSARY. THE MICROSCOPE MUST BE GROUNDED. MAKE SURE THAT THE MAIN
VOLTAGE MATCHES THE VOLTAGE INDICATED ON THE MICROSCOPE BODY.

19
Care and maintenance
• Never, under any circumstances, look directly at the Sun, another bright source of light or at a laser through this
device, as this may cause PERMANENT RETINAL DAMAGE and may lead to BLINDNESS.
• Take necessary precautions when using the device with children or others who have not read or who do not fully understand
these instructions.
• After unpacking your microscope and before using it for the rst time check for integrity and durability of every component
and connection.
• Do not try to disassemble the device on your own for any reason. For repairs and cleaning of any kind, please contact your
local specialized service center.
• Protect the device from sudden impact and excessive mechanical force. Do not apply excessive pressure when adjusting
focus. Do not overtighten the locking screws.
• Do not touch the optical surfaces with your ngers. To clean the device exterior, use only special cleaning wipes and special
optics cleaning tools from Levenhuk. Do not use any corrosive or acetone-based uids to clean the optics.
• Abrasive particles, such as sand, should not be wiped o lenses, but instead blown o or brushed away with a soft brush.
• Do not use the device for lengthy periods of time, or leave it unattended in direct sunlight. Keep the device away from
water and high humidity.
• Be careful during your observations, always replace the dust cover after you are nished with observations to protect the
device from dust and stains.
• If you are not using your microscope for extended periods of time, store the objective lenses and eyepieces separately from
the microscope.
• Store the device in a dry, cool place away from hazardous acids and other chemicals, away from heaters, open re and
other sources of high temperatures.
• When using the microscope, try not to use it near ammable materials or substances (benzene, paper, cardboard, plastic,
etc.), as the base may heat up during use, and might become a re hazard.
• Always unplug the microscope from a power source before opening the base or changing the illumination lamp. Regardless
of the lamp type (halogen or incandescent), give it some time to cool down before trying to change it, and always change it
to a lamp of the same type.
• Always use the power supply with the proper voltage, i.e. indicated in the specications of your new microscope. Plugging
the instrument into a dierent power outlet may damage the electric circuitry of the microscope, burn out the lamp, or
even cause a short circuit.
• Seek medical advice immediately if a small part or a battery is swallowed.
Levenhuk International Lifetime Warranty
All Levenhuk telescopes, microscopes, binoculars, and other optical products, except for their accessories, carry a lifetime
warranty against defects in materials and workmanship. A lifetime warranty is a guarantee on the lifetime of the product on
the market. All Levenhuk accessories are warranted to be free of defects in materials and workmanship for six months from
the purchase date. The warranty entitles you to the free repair or replacement of the Levenhuk product in any country where a
Levenhuk oce is located if all the warranty conditions are met.
For further details, please visit: www.levenhuk.com/warranty
If warranty problems arise, or if you need assistance in using your product, contact the local Levenhuk branch.

20
BG Микроскоп Levenhuk MED PRO 600 Fluo
Описание и работа на микроскопа
Приложение
Микроскопът е предназначен за диагностични изследвания, включително чрез имунофлуоресцентен метод, в клинични,
микробиологични, патологоанатомични и други лаборатории в лечебни заведения. Освен това той може да се използва
и във ветеринарната наука, растениевъдството, биоинженерството, фармацевтичната промишленост, за експертни
изследвания в областта на криминалистиката, държавното епидемиологично наблюдение, опазването на околната
среда. Микроскопът се използва за изследване на оцветени и неоцветени предметни стъкла под формата на намазки и
микросрезове в условия на пропускаща светлина.
В луминесцентната светлина микроскопът дава възможност за откриване на опасни бактериални и вирусни инфекции
при наблюдение на обекти, оцветени с аурамин, акридиново оранжево, FITC и др.
При правилна експлоатация микроскопът е безопасен за здравето, живота, имуществото на потребителя, както и за
околната среда. Стойката на микроскопа е с антивибрационна конструкция. Микроскопът е предназначен за работа
при температура на околната среда от +10 до +35 °С и относителна влажност от максимум 80%. Имерсионният обектив
трябва да работи на закрито при температура от +15 до +25 °С.
Конструкция на микроскопа и принцип на работа
ЗА ДА НЕ ПОВРЕДИТЕ МИКРОСКОПА, ПРЕДИ ИЗСЛЕДВАНЕ ВНИМАТЕЛНО ПРЕГЛЕДАЙТЕ ПРАВИЛАТА ЗА
БОРАВЕНЕ И ПРОЦЕДУРАТА ЗА РАБОТА С МИКРОСКОПА, ПОСОЧЕНИ В НАСТОЯЩОТО РЪКОВОДСТВО ЗА
ЕКСПЛОАТАЦИЯ.
Принципът на действие на флуоресцентния микроскоп се основава на използването на явлението флуоресценция
(луминисценция) на наблюдавани обекти, причинено от светлинни лъчи със специфичен спектър. За да се предизвика
флуоресценция, обектите се осветяват отгоре през лещата на обектива с живачна лампа, използвана като източник на
такава светлина. Светлинният поток, необходим за възбуждане на флуоресценция, се отделя от общото излъчване на
живачната лампа с филтри, условно наричани възбуждащи филтри.
За насочване на светлинния поток към обектива се използва разделител на лъча със специално интерферентно
покритие, което отразява най-вече възбудителната светлина и предава флуоресцентната светлина на обекта.
Възбуждащият филтър, разделителят на лъча и ограничаващият филтър (използван за поглъщане на остатъчното
възбуждащо излъчване) са комбинирани в един модул за разделяне на лъча. Комплект от пет модула за разделяне на
лъча е монтиран на колона със свободно гнездо за работа в условия на пропусната светлина.
Оптичната система, позволяваща изследване на обект във флуоресцентна светлина, е изработена под формата на
подвижен осветител, монтиран на стойката на микроскопа. Стойката на микроскопа позволява наблюдение на обекти,
осветени с пропусната светлина.
Описание и работа на компонентите
Стойка на микроскопа
Стойката на микроскопа (фиг. 1, 12) има ергономична и стабилна форма и е изработена от метал.
На стойката има двустепенен фокусиращ механизъм за вертикално движение на скобата (фиг. 2, 3) с координатна маса
(фиг. 2, 10) и въртяща се револверна глава за фиксиране на обектива (фиг. 2, 7). В горната част на стойката е монтиран
флуоресцентен осветител (фиг. 2, 15) и е фиксиран с винт (фиг. 1, 28). Основата на стойката на микроскопа съдържа
система за осветяване с пропусната светлина и източник на захранване от 12 V/30 W с халогенна лампа. На задната
повърхност на основата вляво има контакт за свързване на захранващ кабел.
Източникът на захранване е вграден в основата на стойката на микроскопа. Бутонът за включване/изключване на
захранването (фиг. 1, 18) захранва халогенната лампа, инсталирана в прожектора (фиг. 1, 13). Захранването е
изключено в положение "O". Нажежаемата жичка на халогенната лампа се регулира с бутон (фиг. 1, 17).
На осветителя има ирисова диафрагма, чийто отвор се регулира чрез пръстен (фиг. 1, 23), разположен на горната
повърхност на основата на микроскопа под кондензатора (фиг. 1, 22). На основата на стойката на микроскопа е
поставена декоративна основа (фиг. 2, 11).
Револверна глава
Шестпозиционната револверна глава осигурява настройка на обектива (фиг. 2, 7) в работно парфокално положение.
Револверната глава е наклонена към стойката на микроскопа, за да се осигури място за поставяне и смяна на
изследваните предметни стъкла.
Лещите на обектива се сменят чрез завъртане на рифелован пръстен (фиг. 1, 27) на револверната глава до фиксирано
положение.
Фокусиращ механизъм
Фокусиращият механизъм е предназначен за вертикално преместване на предметната маса (фиг. 2, 10), когато
микроскопът е фокусиран за контрастен образ на обекта. Диапазонът на движение на предметната маса по височина
е 25 mm. Вертикалното преместване на предметната маса се извършва от коаксиални бутони (фиг. 2, 12 и 13),
разположени от лявата страна на стойката на микроскопа. Бутонът на механизма за фино фокусиране (фиг. 2, 12)
има скала с деление от 2 µm. Зад бутона (фиг. 2, 13) има пръстен (фиг. 2, 14), предназначен да регулира лекотата на