LI-COR Odyssey User manual

Notes on Safety
LI-COR products have been designed to be safe when operated in the manner described in this manual.
The safety of this product can not be guaranteed if the product is used in any other way than is specified
in this manual. The Odyssey Fc Imager is intended to be used by qualified personnel. Read this entire
manual before using the Odyssey Fc Imager.
Equipment Markings:
Manual Markings:
WARNING Warnings must be followed carefully to avoid bodily injury.
CAUTION Cautions must be observed to avoid damaging your equipment.
NOTE Notes contain additional information and useful tips.
IMPORTANT
equipment or related software. Failure to comply may result in a poor experimental
outcome but will not cause bodily injury or equipment damage.
The product is marked with this symbol when it is necessary for you to
refer to the manual or accompanying documents in order to protect
against damage to the product.
The product is marked with this symbol when a hazardous voltage
may be present.
®
Operator’s
Manual

ii
CE Marking:
This product (model number 2800) is a CE-marked product. For conformity information, contact
LI-COR Support at biosupport.licor.com. Outside of the U.S., contact your local sales office or
distributor.

iii
www.licor.com/bio
Notes on Safety
LI-COR products have been designed to be safe when operated in the manner described in this manual. The safety of this
product can not be guaranteed if the product is used in any other way than is specified in this manual. The Odyssey®Fc
Imager is intended to be used by qualified personnel. Read this entire manual before using the Odyssey Fc Imager.
Equipment Markings:
Manual Markings:
WARNING Warnings must be followed carefully to avoid bodily injury.
CAUTION Cautions must be observed to avoid damaging your equipment.
NOTE Notes contain additional information and useful tips.
IMPORTANT Information of importance to prevent procedural mistakes in the operation of the equipment or related
software. Failure to comply may result in a poor experimental outcome but will not cause bodily injury
or equipment damage.
Federal Communications Commission
Radio Frequency Interference Statement
WA R N I N G : This equipment generates, uses, and can radiate radio frequency energy and if not installed in
accordance with the instruction manual, may cause interference to radio communications. It has been
tested and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15
of FCC rules, which are designed to provide a reasonable protection against such interference when oper-
ated in a commercial environment. Operation of this equipment in a residential area is likely to cause inter-
ference in which case the user, at his own expense, will be required to take whatever measures may be
required to correct the interference.
The product is marked with this symbol when it is necessary for you to
refer to the manual or accompanying documents in order to protect against
damage to the product.
The product is marked with this symbol when a hazardous voltage may be
present.

iv
Notice
The information contained in this document is subject to change without notice. LI-COR MAKES NO WARRANTY
OF ANY KIND WITH REGARD TO THIS MATERIAL, INCLUDING, BUT NOT LIMITED TO THE IMPLIED
WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE. LI-COR shall not be
liable for errors contained herein or for incidental or consequential damages in connection with the furnishing, perfor-
mance, or use of this material.
This document contains proprietary information that is protected by copyright. All rights are reserved. No part of this
document may be photocopied, reproduced, or translated to another language without prior written consent of LI-COR,
Inc.
Printing History
Publication Number 977-14589
Revised April 2014
LI-COR, Odyssey, MPX, and IRDye trademarks contained in the Software Product are trademarks or registered trademarks of LI-COR, Inc in the United States
and other countries. Third party trademarks, trade names, and product names may be trademarks or registered trademarks of their respective owners. You may not
remove or alter any trademark, trade names, product names, logo, copyright or other proprietary notices, legends, symbols, or labels in the Software Product. This
EULA does not authorize you to use LI-COR’s or its licensors’ names or any of their respective trademarks.
LI-COR is an ISO 9001 registered company. © 2011 LI-COR, Inc. All rights reserved. Specifications subject to change. LI-COR, Odyssey, MPX, FieldBrite, and
IRDye are trademarks or registered trademarks of LI-COR Inc. in the United States and other countries. The Odyssey Fc Imaging System, FieldBrite XT technology,
and IRDye reagents are covered by U.S. patents, foreign equivalents, and patents pending.

v
End-User License Agreement For LI-COR Image Studio Software
IMPORTANT - READ CAREFULLY: This LI-COR End-User License Agreement (EULA), is a legal agreement
between you (either an individual or a single entity), ("Licensee"), and LI-COR, Inc., having a principal
place of business in Nebraska, ("Licensor" or "LI-COR") for the LI-COR software identified above which
includes computer software, associated media, printed materials and "online" or electronic
documentation ("SOFTWARE PRODUCT"). By installing, copying, or otherwise using the SOFTWARE
PRODUCT, you agree to be bound by the terms of this EULA. If you do not agree to the terms of this
EULA, do not install or use the SOFTWARE PRODUCT; you may, however, return it to LI-COR, Inc. for a
full refund.
SOFTWARE PRODUCT LICENSE
THIRD PARTY SOFTWARE: The SOFTWARE PRODUCT contains third party software ("Third Party
Software") which requires notices and/or additional terms and conditions. Such required Third Party
Software notices and/or terms and conditions are located on the software installation CD and at
http://biosupport.licor.com, and are made a part of and incorporated by reference into this EULA. By
accepting this EULA, you are also accepting the additional terms and conditions set forth therein. THE
SOURCE CODE VERSIONS OF THIRD PARTY ORIGINAL CODE ARE AVAILABLE UNDER THE TERMS AND
CONDITIONS OF EACH THIRD PARTY LICENSE. ANY WARRANTY MADE AVAILABLE UNDER THIS EULA IS
OFFERED BY LI-COR ALONE.
The SOFWARE PRODUCT is protected by copyright laws and international copyright treaties, as well as
other intellectual property laws and treaties. The SOFTWARE PRODUCT is licensed, not sold.
1. GRANT AND SCOPE OF LICENSE. This Agreement grants you the following limited rights:
1.1 Applications Software. You may install and use one copy of the SOFTWARE PRODUCT, or any prior version for the
same operating system, on a single computer.
1.2 Storage/Network Use. You may also store or install a copy of the SOFTWARE PRODUCT on a storage device, such
as a network server, used only to install or run the SOFTWARE PRODUCT on your other computers over an internal
network; however, you must acquire and dedicate a license for each separate computer on which the SOFTWARE
PRODUCT is installed or run from the storage device. A license for the SOFTWARE PRODUCT may not be shared or
used concurrently on different computers.
1.3 Multiple Users License. If you have acquired this Agreement in accordance with a Multiple User's License from LI-
COR, you may make the number of additional copies of the computer software portion of the SOFTWARE PRODUCT
as authorized in writing by LI-COR, and you may use each copy in the manner specified above.
2. DESCRIPTION OF OTHER RIGHTS AND LIMITATIONS. Notwithstanding the license granted above,
Licensor retains all of its ownership and license rights in the Licensed Program (and all Modifications and
Enhancements).

vi
2.1 Not for Resale Software. The SOFTWARE PRODUCT is not available for resale and therefore, notwithstanding
other sections of the Agreement, you may not resell, or otherwise transfer for value, the SOFTWARE PRODUCT.
2.2 Limitations on Reverse Engineering, Decompilation, and Disassembly. You may not reverse engineer, decompile,
adapt, translate, disassemble, or create derivative works based up any portion of the SOFTWARE PRODUCT, except
and only to the extent that such activity is expressly permitted under a Third Party Software license or by applicable
law.
2.3 Separation of Components. The SOFTWARE PRODUCT is licensed as a single product. Its component parts may
not be separated for use on more than one computer.
2.4 Rental. You may not rent, lease, or lend the SOFTWARE PRODUCT.
2.5 Support Services. LI-COR may provide you with support services related to the SOFTWARE PRODUCT ("Support
Services"). Use of Support Services is governed by LI-COR polices and programs described in the user manual, in
"online" documentation, and/or in other LI-COR-provided materials. Any supplemental software code provided to
you as part of the Support Services shall be considered part of the SOFTWARE PRODUCT and is subject to the terms
and conditions of this Agreement. With respect to technical information you provide to LI-COR as part of the Support
Services, LI-COR may use such information for its business purposes, including for product support and development.
LI-COR will not utilize such technical information in a form that personally identifies you.
2.6 Treatment of Licensor Confidential Information. Licensee shall maintain all proprietary and confidential
information embodied in the Software Product, including, without limitation, the Source Code and any Intellectual
Property related thereto ("Confidential Information"), in confidence and shall not use it for any purpose other the
purposes contemplated by this Agreement. In addition, Licensee shall hold all information regarding Licensor's
operations and business systems in trust and confidence for Licensor and shall not use and disclose such information
to any person, and Licensee shall require the same of all of Licensee personnel who engage in work under this
Agreement. This obligation shall survive the expiration and termination of this Agreement. To ensure that this
responsibility is met, Licensee shall instruct all of Licensee personnel and the personnel of Licensee's agents or
permitted assigns who engage in work under this Agreement that they shall keep such information confidential
regardless of whether their relationship with Licensee is terminated at some future time.
2.6.1. Exceptions. Notwithstanding Section 2.5:
2.6.1.1. Licensee may disclose Confidential Information: (a) to those persons who have a need to
know such information to accomplish the purposes of this Agreement; or (b) upon the prior
written approval of Licensor.
2.6.1.2. The obligations of Section 2.5 shall not apply to information that is: (a) in the possession
of Licensee without obligation of confidence to Licensor before receipt thereof from Licensor; (b)
available to the public without fault of Licensor; or (c) is disclosed to Licensee, without restriction,
by a third party who is not under any legal obligation (either by agreement with Licensor or
otherwise) prohibiting such disclosure.
2.6.1.3. Licensee may disclose Confidential Information to governmental agencies or in litigation,
as required by law. Licensee will give Licensor the greatest practicable notice of any such
compelled disclosure.
2.7 Treatment of Licensee Confidential Information by Licensor. Licensor will have no confidentiality obligation with
regard to confidential material or information that is: (a) in the possession of Licensor without obligation of
confidence to Licensor before receipt thereof from Licensee; (b) available to the public without fault of Licensor; or (c)
is disclosed to Licensor, without restriction, by a third party who is not under any legal obligation (either by
agreement with Licensee or otherwise) prohibiting such disclosure. Licensor may disclose Licensee confidential

vii
material to governmental agencies or in litigation, as required by law. Licensor shall give Licensee the greatest
practicable notice of any such compelled disclosure.
2.8. Return of Confidential Information. Upon expiration or termination of this Agreement, each party shall deliver
to the other Confidential Information that is in its possession.
2.9. Irreparable Harm. The parties agree that breach of the above obligations shall be deemed to cause irreparable
harm.
3. UPGRADES/ENHANCEMENTS. If the SOFTWARE PRODUCT is labeled as an upgrade, you must be
properly licensed to use a product identified by LI-COR as being eligible for the upgrade in order to use
the SOFTWARE PRODUCT. A SOFTWARE PRODUCT labeled as an upgrade replaces and/or supplements
the product that formed the basis for your eligibility for the upgrade. You may use the resulting
upgraded product only in accordance with the terms of this Agreement. If the SOFTWARE PRODUCT is
an upgrade component of a package of software programs that you licensed as a single product, the
SOFTWARE PRODUCT may be used and transferred only as part of that single product package and may
not be separated for use on more than one computer.
4. COPYRIGHT. All title and copyrights in and to the SOFTWARE PRODUCT (including but not limited to
any images, photographs, animations, video, audio, and text incorporated into the SOFTWARE
PRODUCT) are owned by LI-COR or its suppliers. The SOFTWARE PRODUCT is protected by copyright
laws and international treaty provisions. Therefore, you must treat the SOFTWARE PRODUCT like any
other copyrighted material except that you may install the SOFTWARE PRODUCT on a single computer
provided you keep the original solely for backup or archival purposes. Also, you may not copy the
printed materials accompanying the SOFTWARE PRODUCT.
5. U.S. GOVERNMENT RESTRICTED RIGHTS. The SOFTWARE PRODUCT and documentation are provided
with RESTRICTED RIGHTS. Use, duplication, or disclosure by the Government is subject to restrictions as
set forth in subparagraph (c)(1)(ii) of the Rights in Technical Data and Computer Software clause at
DFARS 252.227-7013 or subparagraphs (c)(1) and (2) of the Commercial Computer Software - Restricted
Rights at 48 CFR 52.227- 19, as applicable. Manufacturer is LI-COR, Inc./4421 Superior Street/Lincoln,
NE 69504.
6. REPRESENTATIONS AND WARRANTIES
6.1. Warranty.
6.2. Licensor warrants that (a) the Software Product will perform substantially in accordance with the accompanying
written materials for a period of ninety (90) days from the date of receipt.
6.3. Limitations: SUBJECT TO THE ABOVE PROVISION IN SECTION 6.2, LICENSOR DISCLAIMS ALL OTHER
REPRESENTATIONS OR WARRANTIES, EXPRESS, IMPLIED, OR STATUTORY, INCLUDING, BUT NOT LIMITED TO, ANY
IMPLIED REPRESENTATIONS OR WARRANTIES OF MERCHANTABILITY, TITLE, NON-INFRINGEMENT OR FITNESS FOR A
PARTICULAR PURPOSE. IN NO EVENT SHALL LI-COR OR ITS SUPPLIERS BE LIABLE FOR ANY SPECIAL, INCIDENTAL,
INDIRECT OR CONSEQUENTIAL DAMAGES WHATSOEVER (INCLUDING, WITHOUT LIMITATION, LOSS OF BUSINESS
INFORMATION, OR ANY OTHER PECUNIARY LOSS) ARISING OUT OF THE USE OF OR INABILITY TO USE THE SOFTWARE
PRODUCT OR THE PROVISION OF OR FAILURE TO PROVIDE SUPPORT SERVICES, EVEN IF LI-COR HAS BEEN ADVISED OF
THE POSSIBILITY OF SUCH DAMAGES. IN ANY CASE, LI-COR'S ENTIRE LIABILITY UNDER ANY PROVISION OF THIS

viii
AGREEMENT SHALL BE LIMITED TO THE GREATER AMOUNT OF ACTUALLY PAID BY THE LICENSEE FOR THE SOFTWARE
PRODUCT OR U.S. $5.00; PROVIDED, HOWEVER, IF YOU HAVE ENTERED INTO A LI-COR SUPPORT SERVICES
AGREEMENT, LI-COR'S ENTIRE LIABILITY REGARDING SUPPORT SERVICES SHALL BE GOVERNED BY THE TERMS OF
THAT AGREEMENT.
6.4. Exclusive Remedy: TO THE EXTENT THAT THE LICENSOR IS LIABLE, THE EXCLUSIVE REMEDY, AT LI-COR'S OPTION
SHALL BE EITHER (A) RETURN OF THE PRICE PAID, IF ANY, OR (B) REPAIR OR REPLACEMENT OF THE LICENSED
PROGRAM THAT DOES NOT MEET LI-COR'S LIMITED WARRANTY AND WHICH IS RETURNED TO LI-COR WITH A COPY
OF PROOF OF PURCHASE. This Limited Warranty is void if failure of the Software Product has resulted from accident,
abuse, or misapplication. Any replacement Software Product will be warranted for the remainder of the original
warranty period or thirty (30) days, whichever is longer. Outside of the United States, neither of these remedies nor
any product support services offered by LI-COR are available without proof of purchase from an authorized
international source.
7. DISPUTE RESOLUTION
7.1. In the event of a dispute involving the interpretation or application of any provision of this Agreement, the
parties agree not to commence litigation until they have first notified each other of their intent to implement the
terms of this Section after first having employed their best efforts to jointly resolve such dispute. If the parties cannot
resolve their differences in such fashion within thirty (30) days of either party's receipt of such notice of the intent of
the other party to implement the terms of this Section, the following alternative dispute resolution process (the
venue of which shall be Lincoln, Nebraska) shall be immediately implemented:
7.2. Upon written request of either party, the dispute will be referred for negotiation to representatives of the parties
who have no direct operational responsibility for the matters involved in the dispute and who have authority to
resolve the dispute.
7.3. If these representatives have not agreed on a resolution of such dispute within ten (10) Business Days of its
referral to them, the dispute shall be promptly submitted to a neutral adviser (the "Adviser") who shall be chosen
from the list of arbitrators registered with the American Arbitration Association. For purposes of this Section,
"Business Day" shall mean each weekday and the hours of such weekday in which Licensee is open for business. The
Adviser shall, within fourteen (14) days of the submission, recommend, in writing, a procedure for resolving the
dispute and shall specify in such writing whether such procedure shall be binding, non-binding or involve a
combination of binding and non-binding procedures.
7.4. If the parties do not mutually agree upon the process recommended b the Adviser within ten (10) Business Days
of their receipt of the Adviser's written recommendation, they shall promptly convene a non-binding hearing (the
"Mediation"). The rules for Mediation will be established by the Adviser, after consultation with the parties.
7.5. If the dispute cannot be resolved, either through the procedure recommended by the Adviser or through the
Mediation, within such period as the Adviser shall deem reasonable, the Adviser shall, at the request of either party,
certify to the parties that the matter is incapable of resolution.
7.6. No litigation may be commenced concerning the dispute until the Adviser has certified in writing that the dispute
is incapable of resolution, provided that any party may commence litigation: (a) on any date after which such
litigation could be barred by an applicable statute of limitations; or (b) if litigation is otherwise necessary to prevent
irreparable harm to the moving party.
7.7. Each party shall bear its own expenses in connection with the alternative dispute resolution procedures set forth
in this Section, except that the parties shall split equally the fees and expenses of the Adviser, including the costs
associated with any Mediation, and the fees and expenses of any other person designated by the Adviser to assist the
parties.

ix
7.8. All communications made in connection with the alternative dispute resolution procedure set forth in this Section
shall be treated as communications for the purpose of settlement and as such shall be deemed to be confidential and
inadmissible in any subsequent litigation by virtue of Rule 408 of the Federal Rules of Evidence, as the same may be
amended from time-to-time.
8. TERMINATION
8.1. Termination:
8.1.1. By Licensor: Licensor may terminate this Agreement: (a) immediately upon Licensee's copying, or
modification of the Licensed Program, transfer of possession of any copy of the Licensed Program to any
third party, other than as contemplated under this Agreement or otherwise authorized in writing by
Licensor, or other failure to comply with the terms and conditions of this Agreement; or (b) upon thirty (30)
days prior written notice for non-payment results from a good faith dispute between the parties. In such
event, Licensee must destroy all copies of the Software Product and all of its component parts.
8.1.2. By Licensee: Licensee may terminate this Agreement: (a) immediately, upon Licensor's breach of the
obligations in Article 7; or (b) upon thirty (30) days prior written notice thereof to Licensor.
8.2. Bankruptcy Termination: In the event Licensor enters bankruptcy, the laws and rules of the Bankruptcy Code will
govern the enforceability of this agreement.
9. MISCELLANEOUS
9.1. Headings: Unless otherwise stated, all references to Articles and Sections refer to the articles and sections of this
Agreement. The headings of the Articles and Sections of this Agreement are for convenience only and in no way limit
or affect the terms or conditions of this Agreement.
9.2. Governing Law: This Agreement shall be governed by and construed and enforced in accordance with the laws of
the State of Nebraska (without regard to the principles of conflicts of laws embodied therein) applicable to contracts
executed and performable in such state if the product was acquired in the United States. If the product was acquired
outside the United States, then local law may apply.
9.3. Severability: If any provision or any portion of any provision of this Agreement is construed to be illegal, invalid
or unenforceable, such provision or portion thereof shall be deemed stricken and deleted from this Agreement to the
same extent and effect as if it were never incorporated herein, but all other provisions of this Agreement and the
remaining portion of any provision that is construed to be illegal, invalid or unenforceable in part shall continue in full
force and effect; provided that the resulting construction of the Agreement does not frustrate the main purpose of
the Agreement.
9.4. Entire Agreement: This Agreement constitutes the entire agreement between the parties and supersedes all
previous agreements, promises, representations, understandings and negotiations, whether written or oral, between
the parties with respect to the subject matter hereof. Any modification and/or amendment to this Agreement must
be in writing and executed by both parties.
9.5. Survival: The provisions of Articles 1,2,6 and 7 shall survive termination or expiration of the Agreement.
9.6. Successors and Assigns; Change of Control: All the terms and conditions of this Agreement are binding upon and
inure to the benefit of the parties hereto, their successors, legal representatives, and permitted assigns. Licensee
may transfer, lease, assign or sublicense its entire right, interest and obligation hereunder to any third party who
enters into a substitute version of this Agreement.

x
9.7. No Relationship Between the Parties: Neither party shall represent itself as the agent or legal representative of
the other or joint venture for any purposes whatsoever, and neither shall have any right to create or assume any
obligations of any kind, express or implied, for or on behalf of the other in any way whatsoever.
9.8. Non-Waiver: A failure of either party to enforce at any time any term, provision, or condition of this Agreement,
or to exercise any right or option herein, shall in no way operate as a waiver thereof, nor shall any single or partial
exercise preclude any other right or option herein, in no way whatsoever shall a waiver of any term, provision or
condition of this Agreement be valid unless in writing, signed by the waiving party, and only to the extent set forth in
such writing.
9.9. Notices: Unless expressly stated otherwise, all notices required herein shall be given in writing and shall be
delivered (and notice shall be deemed effective upon delivery) in person, by courier, or sent by certified United States
mail, postage prepaid, return receipt requested, to the following address:
LI-COR, Inc., 4647 Superior Street, P.O. Box 4000, Lincoln, Nebraska, 68504 USA
LI-COR, Odyssey, MPX, and IRDye trademarks contained in the Software Product are trademarks or
registered trademarks of LI-COR, Inc. in the United States and other countries. Third party trademarks,
trade names, and product names may be trademarks or registered trademarks of their respective
owners. You may not remove or alter any trademark, trade names, product names, logos, copyright or
other proprietary notices, legends, symbols, or labels in the Software Product. This EULA does not
authorize you to use LI-COR’s or its licensors’ names or any of their respective trademarks.
LI-COR is an ISO9001 registered company. ©2011 LI-COR, Inc. All rights reserved. Specifications subject
to change. LI-COR, Odyssey, MPX, and IRDye are trademarks or registered trademarks of LI-COR, Inc.

xi
Table of Contents
Chapter 1: Installation and Safety Information
Safety Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Laser Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Safety Interlocks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Placement in the Laboratory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Ambient Laboratory Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Instrument Ventilation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Space Requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Moving the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Chemical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Electrical Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Power Cords . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Fuse Replacement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Chassis Ground Access Hole . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Chapter 2: System Overview
External Panels and Controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
Right-side and Rear Panels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Imaging Drawer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Imaging Drawer Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Sample Placement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Using Membranes. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Using Gels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Optical System Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Computer Connections and Networking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Image Studio Software Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Learning the Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11

xii
Chapter 3: Maintenance
Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Cleaning the Fan Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
Front Panel Warning Light Indicates an Error . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
Resetting the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
Desiccant Replacement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
Obtaining Technical Support. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
Chapter 4: Appendix
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .17

Page 1
www.licor.com/bio
Chapter 1 Installation and Safety Information
Safety Considerations
Laser Safety
The Center for Devices and Radiological Health (CDRH) was established in October 1982, by the U.S. Food and Drug
Administration (FDA) to protect the public health in the fields of medical devices and radiological health.
Manufacturers of products subject to performance standards under the Radiation Control for Health and Safety Act of
1968 are required to furnish various reports to the CDRH.
The Odyssey®Fc Imager is certified as a Class I laser product. This means that hazardous laser radiation is not emitted
outside the instrument. Radiation emitted inside the Odyssey Fc is confined within protective housings and external cov-
ers. A series of safety interlocks ensures that the laser beam cannot escape during any phase of user operation.
One laser emits at 785nm and the other one at 685-690nm. The 785nm laser has a peak power rating of
1.2 watt. The 685-690nm laser has a peak power rating of 0.5 watt. The lasers emit visible and invisible laser radiation -
direct exposure to either beam may cause eye damage. Laser radiation from each laser uniformly illuminates the tar-
get area only, which is marked in the imaging tray (Figure 2-6) that fits in the imaging drawer. There is no emission of
laser radiation outside the instrument under normal conditions.
The CDRH implemented regulations for laser products on August 1, 1976 (CDRH radiation performance standard 21,
Code of Federal Regulations Chapter 1, Subchapter J). Compliance for products marketed in the United States is manda-
tory. The label that must be attached to laser products marketed in the United States is Figure 1-1 and is located on the
rear panel of the Odyssey Fc (Figure 1-2), indicating compliance with CDRH regulations.
WARNING: Use of controls or adjustments or performance of procedures other than those specified herein may result
in hazardous radiation exposure.

Chapter 1: Installation and Safety Information
Page 2
Safety Interlocks
Figure 1-1. CDRH and IEC 60825-1:2007 regula-
tion compliance label.
Figure 1-2. (a) Location of CDRH compli-
ance label on the right side of the Odyssey
Fc instrument. (b) Access hole for chassis
ground.
(a)
(b)
DANGER - CLASS 4 VISIBLE AND INVISIBLE
LASER RADIATION WHEN OPEN
AVOID EYE OR SKIN EXPOSURE TO DIRECT
OR SCATTERED RADIATION
DANGER
DANGER - APPAREIL LASER DE CLASSE 4.
RAYONNEMENT LASER VISIBLE ET INVISIBLE EN CAS D’OVERTURE.
EVITER L’EXPOSITION DE L’OEIL OU DE LA PEAU
AU RAYONNEMENT DIRECT OU DIFFUS.
Where there is a potential for class 4 laser radiation behind a
given panel, the label in Figure 1-3 is affixed to the panel. The
label shown in Figure 1-3 is affixed to the Odyssey®Fc Imager
Instrument in two locations; one on top of the camera hood and
one on the inner front access panel (Figure 1-4).
Figure 1-3. Class 4 laser warning
label.
Figure 1-4. Location of class 4 laser warning label located on (a) the camera hood
under the top panels of the Odyssey Fc instrument enclosure and (b) on the inner front
access panel (do not open, no user-serviceable parts inside).
(a)
(b)

Odyssey
®
Fc Imager
Page 3
www.licor.com/bio
The Odyssey®Fc Imager has two safety interlocks that prevent exposure to laser radiation when the imaging drawer is
opened during operation. Do not attempt to defeat these interlocks. The drawer interlocks are located in the back of the
instrument.
Placement in the Laboratory
The Odyssey Fc Imager weighs approximately 27 kg (60 lbs). The instrument should be placed on a laboratory bench
that is sufficiently sturdy to bear its weight. The bench should also be able to bear the weight of the computer if the com-
puter is to be installed beside the Odyssey Fc instrument.
Ambient Laboratory Conditions
Place the Odyssey Fc Imager away from external heat sources (furnaces, windows, etc.). Additional heating can cause
high temperatures within the enclosure. Place the instrument away from sinks or other sources of water that pose a shock
hazard. Recommended operating conditions are 15-35°C and a dew point not greater than 22°C to prevent condensation
on the laser assemblies, which are cooled during operation.
Instrument Ventilation
The air intake fan on the back of the Odyssey Fc instrument (Figure 2-4) is used to cool the internal electronics and
maintain fresh air circulation throughout the internal volume of the instrument. The air intake is filtered and the filter
should be periodically cleaned (Chapter 3). Make sure there is plenty of room behind the instrument so that the air intake
is not blocked and the filter is easily accessible for cleaning.
Space Requirements
The Odyssey Fc instrument requires an area 41.4cm W (16.3") x 47cm D (18.5") x 67.3cm H (26.5"). Depth with the
imaging drawer open is 59.7cm (23.5”).
Moving the Instrument
Be cautious and use a minimum of two people, one on each side of the instrument, when moving the
LI-COR®Odyssey Fc Imager, as it weighs about 27 kg (60 lbs). Each person should place one hand in the lower hand
hold and the other at the top of the instrument to help stabilize it. Keep the instrument as near vertical as possible. Gently
set the instrument at its new location.
After the instrument has been moved, use the adjustment feet on the bottom of the instrument to approximately level the
instrument. The instrument does not need to be level for operation, but if the instrument is not sufficiently level, liquid in
the imaging tray may be uneven and pool in one of the corners or sides. Each of the four feet on the bottom of the instru-
ment case is threaded and can be turned in either direction to adjust its height. The instrument should not rock back and
forth after you are finished.
Chemical Safety
LI-COR Biosciences recommends that all biochemicals be handled carefully, and that safe laboratory procedures be fol-
lowed at all times. Be aware of the hazards associated with any chemical before you begin work.
The LI-COR®Odyssey®Fc Imager should not be used with any radioactive materials.

Chapter 1: Installation and Safety Information
Page 4
Electrical Considerations
Power Cords
The Odyssey®Fc Imager is equipped with a 3-wire grounding-type plug. This plug will only fit into a grounding-type
outlet. This is a necessary safety feature. If you are unable to insert the plug into the outlet, you will need to replace the
outlet. Do not defeat the purpose of the grounding-type plug.
Do not locate the Odyssey Fc Imager where the power cord will be walked on or exposed to water or chemical spills.
The Odyssey Fc Imager draws a maximum of 2 amperes at 120V (see Chapter 4 for complete specifications). If an
extension cord is used, make sure the total of the ampere ratings on the instruments plugged into the extension cord does
not exceed the extension cord ampere rating. Also make sure the total amperage of instruments plugged into the wall
outlet does not exceed the amperage capacity for the outlet (usually 15 or 20 amperes in the United States).
Disconnect power before servicing.
Fuse Replacement
There are no user replaceable fuses in this instrument.
Chassis Ground Access Hole
To eliminate the potential for ESD damage during service, a hole on the side of the control module provides a chassis
ground (Figure 1-2).

Page 5
www.licor.com/bio
Chapter 2 System Overview
External Panels and Controls
Front Panel
Power On/Off Switch: Pressing this switch momentarily turns the instrument on and off. Pressing the switch for 5
seconds or more cuts power to the instrument (Chapter 3). While the instrument is performing its startup or shutdown
procedure, the blue indicator light in the power switch blinks. When the blue indicator light is continuously on, the
Power on/off button
Imaging drawer
open/close button
Imaging drawer
(closed)
Red error
condition
indicator
(shown unlit)
Green image acquisition
indicator (shown unlit)
Figure 2-1. Front panel features and controls of the Odyssey®Fc Imager.
Imaging tray storage
shelf

Chapter 2: System Overview
Page 6
instrument is ready for operation. Pressing the Power On/Off button when the green image acquisition indicator is lit will
cause the red error condition indicator to illuminate. This is just a warning that the instrument is in use.
Green Image Acquisition Indicator: This indicator light blinks during image acquisition and is continuously on at other
times, as long as communication with the Image Studio software is maintained. The indicator light is off only if commu-
nication is lost or the Image Studio software is closed or the instrument is powered off.
Red Error Indicator: The error indicator light illuminates when a particular process could not be completed. If the
imaging drawer button is pressed before an acquisition is complete, for example, the error indicator illuminates. See
instrument diagnostic log in the Image Studio software for details of what error occurred. See Chapter 3, Maintenance.
Imaging Drawer Button: This button opens and closes the imaging drawer in normal operation. The drawer front can
be used to open or close the drawer in cases of power loss. Pressing the Imaging Drawer button when the green image
acquisition indicator is lit will cause the red error condition indicator to illuminate. This is just a warning that the
instrument is in use.
Imaging Drawer: The imaging drawer moves the imaging tray into position under the camera for imaging. The bottom
of the imaging tray has a marker in each corner that indicates the edge of the camera’s field of view.
Figure 2-2. Location of the
green image acquisition light
and red error light.
Green image acquisition indicator
Red error indicator

Odyssey
®
Fc Imager
Page 7
www.licor.com/bio
Right-side and Rear Panels
Figure 2-3. Close up of the Odyssey®Fc control
module showing the power receptacle and ethernet
connectors (use only the supplied ethernet cable).
All other connectors on this panel should not be
used.
Power Receptacle
Ethernet
connectors
Power Receptacle
Ethernet
connectors
Figure 2-4. Rear panel showing air intake fan.
Keep the intake unblocked during operation
and accessible for routine filter cleaning
(Chapter 3).
Air intake fan

Chapter 2: System Overview
Page 8
Imaging Drawer
The imaging drawer is opened and closed by pressing the large button above the drawer (Figure 2-1).
Imaging Drawer Safety
Be careful not to get your fingers caught between the Odyssey®Fc instrument and the imaging drawer when the drawer
closes. The drawer is specially designed to stop when a certain level of resistance is detected, so no harm should result,
but the experience may be startling.
During an unexpected power loss, the drawer button will not function. This may leave a sample inside the Odyssey Fc
Imager, or it may result in the imaging drawer being left partially open or closed. If the power remains off, the drawer can
be opened or closed by pulling or pushing on the drawer front. The imaging drawer should resume normal operation once
power is restored.
Figure 2-5. The imaging drawer holds one imaging
tray that is capable of containing one membrane or
gel up to 10 x 12 cm.
Imaging drawer
(open)
Imaging tray