LLG uniSPEC 1 UV/VIS User manual

LLG-uniSPEC 1 UV/VIS
Spektrophotometer
Thank you very much for purchasing
this LLG-uniSPEC 1 UV/VIS.
Please read the following instructions carefully
and always keep this manual within easy reach.
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OPERATION MANUAL
OPERATION MANUAL EN

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Contents
1. Preface 3
1.1 Safety 3
1.2 General 3
1.3 Electrical 4
1.4 Warning 4
1.5 Performance 4
1.6 Radio Interference 4
2. Introduction of the Instrument 5
2.1 Brief Introduction of the instrument 5
2.2 Working Principle 6
2.3 Structure 6
2.4 Unpacking Instructions 7
2.5 Specications 7
3. Install Instrument 8
3.1 Environment Required 8
3.2 Installation 8
4. Operation Introduction 9
4.1 Software Structure 9
4.1.1 Main Interface 9
4.1.2 Firmware Functions 9
4.2 Basic Operation 10
4.2.1 Set Wavelength 10
4.2.2 Delete the test result and stored data 10
4.2.3 Calibrate 100%T/0Abs 10
4.2.4 Measure Samples 10
4.2.5 Print the test result 10
4.3 Preparation before test 10
4.3.1 Switch on the instrument and begin the system self-test 10
4.3.2 Pre-warm 10
4.3.3 Check the cuvettes 10
4.4 Measurement 11
4.4.1 T-Mode 11
4.4.2 A-Mode 12
4.4.3 C-Mode (Standard Curve) 12
4.4.4 F-Mode (Coefcient Method) 16

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Contents
Read the following manual before installing and using the instrument and its accessories.
This instrument should be operated by appropriate laboratory technicians.
The apparatus described in this manual is designed to be used by properly trained personnel
in a suitable equipped laboratory. For the correct and safe use of this apparatus it is
essential that laboratory personnel follow generally accepted safe procedures in addition to
the safety precautions called for in this manual.
The covers on this instrument may be removed for servicing. However, the inside of the
power supply unit is a hazardous area and its cover should not be removed under any
circumstances. There are no serviceable components inside this power supply unit. For this
instrument, avoid touching the high voltage power supply at all times.
Some of the chemicals used in spectrophotometry are corrosive and/or inammable and
samples may be radioactive, toxic, or potentially infective. Care should be taken to follow
the normal laboratory procedures for handling chemicals and samples.
1. Preface
1.1 Safety
1.2 General
5 System Functions 18
5.1 Energy 18
5.2 Get Dark Current 19
5.3 Wavelength Calibration 20
5.4 Load Default 20
5.5 Version 21
6. Instrument Maintenance 21
6.1. Daily Maintenance 21
6.2. Trouble Shooting 22
6.3. Fuse replacement 22
7. Spare part and accessories 23
8. Warranty 24

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Before switching on the apparatus, make sure it is set to the voltage of the local power
supply (see Installation).
The power cord shall be inserted in a socket provided with a protective earth contact. The
protective action must not be negated by the use of an extension cord without a protective
conductor.
Any interruption of the protective conductor inside or outside the apparatus or disconnection
of the protective earth terminal is likely to make the apparatus dangerous. Intentional
interruption is prohibited.
Whenever it is likely that the protection has been impaired, the apparatus shall be made
inoperative and be secured against any unintended operation.
Note: NEVER touch or handle the power supply on this instrument due to the high voltage!
The protection is likely to be impaired if, for example, the apparatus
• Shows visible damage
• Fails to perform the intended measurements
• Has been subjected to prolonged storage under unfavorable conditions
• Has been subjected to severe transport stresses
To ensure that the instrument is working within its specication, especially when making
measurements of an important nature carry out performance checks with particular
reference to wavelength and absorbance accuracy. Performance checks are detailed in this
manual.
For compliance with the EMC standards referred to in the EC Declaration of Conformity, it is
necessary that only shielded cables supplied by us are used when connecting the instrument
to computers and accessories.
1.3 Electrical
1.4 Warning
1.5 Performance
1.6 Radio Interference

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The LLG-uniSPEC 1 is a single beam, general purpose instrument designed to meet the
needs of the Conventional Laboratory, This instrument is ideal for various applications, such
as: Chemistry, Biochemistry, Petro-chemistry, Environmental Protection, Food and Beverage
Labs, Water and Waste Water Labs and other elds of quality control and research.
This instrument incorporates a 128 × 64 dots matrix LCD display for photometric results,
easy operation and wavelength range of 190 nm to 1000 nm. This instrument is ideal
for measurements in the visible and ultraviolet wavelength region of the electromagnetic
spectrum.
2. INTRODUCTION OF THE INSTRUMENT
2.1 Brief Introduction of the instrument
Fig 2-2 Control Panel
Fig 2-1 Front View

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Different matter has different but special absorbance wavelength point. Also, when at
the xed wavelength point, the absorbance has some relation to the substance’s (Always
transparent Solution) concentration and its thickness. The relation can be concluded as the
following Formula, which is called Lambert-Beer Law.
T= I/Io
A=KCL= -log I/Io
A Absorbance
C Concentration of the Solution
K Absorbance Coefcient of the Solution
L The length of the Solution in the light path
I The intensity of the light focused on the A/D after it permeate the solution to be
measured.
Io The intensity of the light focused on the A/D after it permeate the Solution.
Note: When the test is running, the solvent is usually taken as the Reference Solution and its
Transmittance is considered as 100%T. While the Transmittance of the sample to be tested is
a relative value which is going to be compared to that of the Reference.
The spectrophotometer consists of ve parts:
1) Halogen or deuterium lamps to supply the light;
2) A monochromator to isolate the wavelength of interest and eliminate the unwanted
second order radiation;
3) A sample compartment to accommodate the sample solution;
4) Detector to receive the transmitted light and convert it to an electrical signal; and
5) A digital display to indicate absorbance or transmittance. The block diagram (Fig 2.4)
below illustrates the relationship between these parts.
In your spectrophotometer, light from the lamp is focused on the entrance slit of the
monochromator where the collimating mirror directs the beam onto the grating. The grating
disperses the light beam to produce the spectrum, a portion of which is focused on the exit
slit of the monochromator by a collimating mirror. From here the beam is passed to a sample
compartment through one of the lters, which helps to eliminate unwanted second order
radiation from the diffraction grating. Upon leaving the sample compartment, the beam is
passed to the silicon photodiode detector and causes the detector to produce an electrical
signal that is displayed on the digital display.
2.2 Working Principle
2.3 Structure
Light
Scource
Monochromator DetectorSample
Compartment
Process and
Display
Fig 2.4 Block diagram for the Spectrophotometer

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Carefully unpack the contents and check the materials against the following packing list to
ensure that you have received everything in good condition.
2.4 Unpacking Instructions
2.5 Specications
Packing List
Description Quantity
Spectrophotometer 1
Power Cord 1
1 cm Glass Cuvettes 1 Set of 4
1 cm Quartz Cuvettes 1 Set of 2
User’s Manual 1
Wavelength Range 190-1000 nm
Band Width 2 nm
Wavelength Accuracy ±2 nm
Wavelength Repeatability 0.5 nm
Wavelength Setting Auto
Photometric Accuracy ±0.5 %T
Photometric Repeatability ≤0.2 %T
Photometric Display Range 0-200 %T, -0.3-3.0A, 0-9999C
Stability 0.002 A/h @ 500 nm
Stray Light ≤0.1 %T
Data Output Port USB
Printer Port Parallel Port
Display 128*64 Dots LCD
Lamps Deuterium Lamp & Tungsten Halogen Lamp
Detector Silicon Photodiode
Power Requirement AC85~250 V
Dimension (L*W*H) 420*280*180 mm
Weight 11 kg
Note: The Printer mentioned in this manual is an optional accessory. It does not come
standard with the instrument.

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To ensure the best performance, the following conditions are required:
• The best working temperature range is 16-30 °C and the humidity is 45-80 % r.H.
• Keep it as far as possible away from the strong magnetic or electrical elds or any
electrical device that may generate high-frequency elds.
• Set the unit up in an area that is free of dust, corrosive gases and strong vibrations.
• Remove any obstructions or materials that could hinder the ow of air under and around
the instrument.
• The power requirement is AC85V~265V.
• Use the appropriate power cord and plug into a grounded outlet.
• If the local voltage is not stable enough, a Power Stabilizer is required.
• Keep away from direct sunlight
Step 1: Check the packing list
Unpack the contents, check the materials with the Packing List. Any damage or
item missing please contact us or the local dealer.
Step 2: Position
Place the instrument on a stable table carefully.
Step 3: Link the power cord
Make sure the instrument’s power switch is in the Off condition, link the power
cord to the instrument and insert another end in the socket provided with a
protective earth contact.
Step 4: Switch on the power
Check again, make sure that connections are correct. Switch on the power.
The instrument can be used for measurements after self-test and pre-warm.
3.1 Environment Required
3.2 Installation
3. Install Instrument

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After 20 minutes’ warm-up, the system goes into main interface. (Fig.4-1) Press the key of
MODE to change the functions.
The rmware consists of 5 functions: T, A, C, F and system functions
1) T
To measure the Transmittance of a sample in a xed wavelength point. The test result can be
stored in the Photometer, total 200 groups of data can be stored.
2) A
To measure the Absorbance of a sample in a xed wavelength point. The test result can be
stored in the Photometer, total 200 groups of data can be stored.
3) C-Standard Curve Method
Set up standard curve by standard samples; use the new curve to measure the unknown
sample’s concentration. The curves and the test results can be stored in the Photometer. Total
200 curves can be stored.
4) Coefcient Method
Input the values of the curve equation’s coefcient, and then measure the unknown sample’s
concentration.
5) Setup-System Functions
Press the key of SET on the keypad to go into system functions (Only available in T MODE
and A MODE). Light source management; Get Dark Current; Calibrate Wavelength and Load
default, etc.
4.1 Software Structure
4.1.1 Main Interface
4.1.2 Firmware Functions
4 Operation Introduction
Fig 4-1 Main Interface

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In the measurement Interface, press GOTO λto go into wavelength setting Interface. Use
Arrow Key to increase or decrease the value of wavelength, then press ENTER to conrm.
When arriving at the setting wavelength point, it sets 100%T/0Abs automatically.
In the measurement Interface, press CLEAR to delete the test result and stored data.
Put the Reference in the light path, press ZERO to calibrate 100%T/0Abs.
Put the samples in the light path and press ENTER to measure
In the Measurement Interface, press PRINT to print the result.
4.2 Basic Operation
4.2.1 Set Wavelength
4.2.2 Delete the test result and stored data
4.2.3 Calibrate 100%T/0Abs
4.2.4 Measure Samples
4.3.1 Switch on the instrument and begin the system self-test
4.3.2 Pre-warm
4.3.3 Check the cuvettes
4.2.5 Print the test result
Make sure to remove all the cuvettes in the light path; Close the compartment lid
and switch on the power; Then the system will begin self-test.
Note: During the course of Diagnostic Program runs, please DO NOT open the lid of
the compartment!
When the diagnostic program nished, it goes into pre-warm condition. 20 minutes is
required before measure.
The cuvette should be clear and no solution should remain on the transparent surface. If the
wavelength is below 340nm, you will have to use a Quartz Cuvette.
4.3 Preparation before test

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4.4.1 T-Mode
Step 1, Go into T Mode Interface
In the Main Interface, press MODE Key to move the cursor on “T”, you will see the current
sample’s T% value. Press ENTER , to go into Continuous Measurement Interface. Fig. 4-2;
Step 2, Set Wavelength
Press GOTO λto set Wavelength, input the wavelength value by the Arrow
Key, then press ENTER to conrm. Fig. 4-3;
4.4 Measurement
Fig. 4-2
Fig. 4-3
Fig. 4-4
Step 3, Calibrate 100%T/0Abs
Put the Reference Solution in the light path, press ZERO to calibrate 100%T/0Abs;
Step 4, Measure Sample
Put the unknown concentration sample in the light path, press S TART to
measure, and the result displays in the data sheet. At the same time, the test result will be
stored in the Photometer automatically.
Repeat this step to nish all the samples measurement.
Step 5, Print or Delete the data
Press PRINT/CLEAR to go into the following interface.(Fig.4-4)

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1) When the optional Printer is linked to the instrument, if you want to print out the test
result, use Arrow Key to choose “Print”, then press ENTER to conrm. After printing, the
data will be deleted from the Photometer.
2) If you want to delete the saved data, move the cursor on the item of “Delete”, then press
ENTER to conrm.
1) Go into C-MODE Interface
In the Main Interface, press Arrow Key to move the cursor on “C”, then you will see the
following Interface. (Fig.4-6) Here you can create a new curve, or you can load a saved
curve. If you want to delete a saved curve, just choose “Delete Curve”.
4.4.2 A-MODE
4.4.3 C-MODE (Standard Curve)
Step 1, Go into A Mode Interface
In the Main Interface, press MODE Key to move the cursor on “A”, you will see the current
sample’s Absorbance value. Press ENTER to go into Continuous Measurement Interface.
Fig. 4-5;
Step 2-5 is the same as T-mode, please refer to the corresponding operation in
4.4.1
Fig. 4-5
Fig. 4-6

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Fig. 4-7
Fig. 4-8
Fig. 4-9
2) Create Curve
If you want to create a new standard curve by several standard samples, Use Arrow Key to
move the cursor on “Create Curve”, then press ENTER to conrm. The system will ask you to
input the quantity of your standard samples. (Fig.4-7).
Note: The default quantity is “3”. Please don’t change the value at this time, you should rst
set the Wavelength.
Step 2, Input the quantity of the standard samples
Use Arrow Key to input the quantity of the standard samples, then press ENTER to conrm.
The system will go into the following interface. (Fig. 4-9)
Step 1, Set Wavelength
Press GOTO λto go into the wavelength setting interface, input the WL. Value by the Arrow key
and press ENTER to conrm. Fig. 4-8;
Step 3, Calibrate 100%T/0Abs
Put the Reference Solution in the light path, then press ZERO to calibrate 100%T/0Abs

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Step 4, Input Standard Samples’ Concentration
Pull the 1# Standard Sample in the light path and input the value of its concentration by the
Arrow key, then press ENTER to conrm. The system will go into the next interface.
(Fig.4-10)
Step 5, Display the Curve
When the last standard sample is nished, double press ENTER to conrm. then the Standard
Curve and its equation will display on the screen automatically. (Fig. 4-11) At the same time,
the Curve Equation will be saved in the Photometer automatically.
Step 6, Measure Samples
Put the Reference solution in the light path and press ENTER to go into the continuous
measurement interface. (Fig. 4-12)
Note: If a mistake happens during the operation, the system will beep 3 times and return the
initial interface automatically. Of course, the curve can not be displayed on the screen.
Put the unknown sample in the light path, then press START to measure.
The result will be displayed on the screen one by one. At the same time, the data will be
saved in the Photometer automatically. Total 200 groups of data can be saved.
Pull the 2# Standard Sample in the light path and input its concentration, then press
ENTER to conrm.
Repeat the above step till the last sample’s concentration is nished.
Fig. 4-10
Fig. 4-11
Fig. 4-12

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3) Load Curve
All the curve equations will be saved in the Photometer automatically. If you want to reload
the saved curve, just follow the following steps:
Move the cursor on “Load Curve” and press ENTER to conrm. Then the system will go into
the curve equation record interface.(Fig. 4-13)
4) Delete Curve
Move the cursor on “Delete Curve” and press ENTER to conrm. Then the system goes into
the curve equation selection interface. (Fig. 4-15)
Fig. 4-13
Fig. 4-15
Fig. 4-14
Move the cursor on the equation that you need and press ENTER to conrm. Then the
corresponding curve will display on the screen. (Fig. 4-14)
Press ENTER to go into sample continuous measure interface and
measure samples. (Fig. 4-12)
Note: When you reload the curves, the wavelength will be set at the point where you create
the Standard Curve automatically.

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Move the cursor on the curve equation that you want to delete and press ENTER , then system
will ask you to conrm again. (Fig. 4-16)
Move the cursor on “Yes” and press ENTER to conrm, then the curve equation will be
deleted. If you don’t want to delete it, just choose “No” or press ESC to return.
Fig. 4-16
Fig. 4-18
1) Go into F-MODE Interface
Press Arrow Key to choose “F” in the Main Interface, and you’ll see the following Interface.
(Fig. 4-17)
2) Parameter Setting
Use Arrow Key to move the cursor on “Coefcient K” and press ENTER to go into K setting
interface. Fig. 4-18; Enter every digit of the K value using the Arrow Key and press ENTER
to conrm. When the last digit is entered, the system will return to the previous Interface
automatically.
4.4.4 F-MODE (Coefcient Method)
Fig. 4-17

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Input the value of Bin the same way(Fig. 4-19)
3) Test Samples
Step 1, Go into pre-test Interface
Move the cursor on “Test”, then press ENTER to go into pre-test Interface. (Fig.4-20)
Step 2, Set Wavelength
Press GOTO λto go into wavelength setting interface, input the wavelength value by
Arrow key and press ENTER to conrm. (Fig. 4-21)
Fig. 4-19
Fig.4-20
Fig. 4-21

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Step 3, Calibrate 100%T/0Abs
Press ENTER to go into continuous measurement interface (Fig. 4-22). Put the Reference
Solution in the light path, then press ZERO to calibrate 100%T/0Abs;
Step 4, Measurement
Put the samples to be measured in the light path, press ENTER to measure, then the result
will be displayed on the screen . Repeat this step to measure other samples. All the test
results will be listed on the screen and stored in the Photometer automatically. They can be
reload even after a sudden power cut.
Step 5, Print or Delete the Test Result
In the continuous measurement interface, press ENTER/CLEAR to print or delete the test result.
Please refer to Chapter 4.1 for details.
Fig. 4-22
In the Tmode or Amode of Main Interface, press SET Key to go into system
functions Fig. 4-23).
To see the energy of present wavelength. Move the cursor on “Energy”, then press ENTER to
conrm. (Fig.4-24)
Note: Users seldom use this function. It is useful for service to judge the fault.
5. System Functions
Fig. 4-24
Fig. 4-23
5.1 Energy

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Use Arrow key to choose “Dark Current” and press ENTER to conrm (Fig. 4-25)
Note: Remove the cuvettes from the light path rst. Do not open the lid of the
compartment during the course of calibration.
Press ESC to return when it nishes.
Fig. 4-25
When the working condition changes, a dark current calibration
is required before any measurement.
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When it nishes, it will display the following words on the screen. (Fig. 4-26). If it displays
“Dark Current Err”, you should check if there’s something else in the compartment and
repeat this step.
Fig. 4-26
5.2 Get Dark Current

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Remove all the cuvettes from the light path and close the lid of the compartment, press
Arrow Key to choose “Calibrate WL.” and press ENTER to begin the calibration (Fig. 4-27).
The lid of the compartment should not be opened during the calibration.
Press Arrow Key to choose “Load Default” and press ENTER to conrm. (Fig. 4-29) press Arrow
Key to choose “Yes” and press ENTER to conrm.
Then the system will recall the default settings. Choose “No” to cancel the operation.
When it nishes, it will display the following words on the screen. (Fig. 4-28). If it displays
“Wavelength Err”, you should check if there’s something else in the compartment and repeat
this step.
Note: All the saved data and curves would be deleted from the Photometer once “Yes” is
chosen!
Fig. 4-27
Fig. 4-29
Fig. 4-28
After some time, the energy of lights drops off which can have a
slight inuence on the test result. In this case, users can reset the
wavelength to compensate. We suggest the users to calibrate the
wavelength every one or two months.
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5.3 Wavelength Calibration
5.4Load Default