LLG LLG-uniSPEC 2 User manual

LLG-uniSPEC 2
Please read the User Manual carefully before use, and follow all
operating and safety instructions!
LABWARE
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INSTRUCTION MANUAL
INSTRUCTION MANUAL UK

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1. Preface
1. Safety ........................................ 3
2. General ....................................... 3
3. Electrical ..................................... 4
4. Warning ...................................... 4
5. Performance ................................... 4
6. Radio Interference ............................... 4
2. Introduction of the Instrument
1. Brief Introduction of the Instrument................... 5
2. Working Principle ................................ 6
3. Structure...................................... 7
4. Unpacking Instructions ............................ 7
5. Specications................................... 8
3. Install Instrument
1. Environment Required............................. 9
2. Voltage Conrmation ............................. 9
3. Installation ....................................10
4 Operation Introduction
1. Software Structure ...............................11
1.1. Menu Tree ................................11
1.2. Firmware Functions ........................ 12
1.2.1 Basic Mode .............................. 12
1.2.2 Quantitative.............................. 12
1.2.3 Kinetics ................................. 12
1.2.4 Setup .................................. 12
2. Basic Operation ................................ 12
2.1 Choose Photometric Mode .................... 12
2.2 Set Wavelength ........................... 12
2.3 Set Parameters ........................... 12
2.4 Set the position of auto-cell holder.............. 12
2.5 Delete the Input Value ...................... 12
2.6 Delete the test result and stored data ........... 12
2.7 Calibrate 100%T/0Abs....................... 12
2.8 Measure Samples .......................... 12
2.9 Print the test result ........................ 12
3. Preparation before test ........................... 13
3.1 Switch on the instrument and begin
the system self-test ........................ 13
3.2 Pre-warm ............................... 13
3.3 Check the cuvettes......................... 13
4. Measurement.................................. 13
4.1 Basic Mode .............................. 13

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4.2 Quantitative.............................. 15
4.3 Kinetics ................................. 21
4.4 Setup ...................................24
4.4.1 Power On/O D2 Lamp..................24
4.4.2 Power On/O W Lamp ................. 25
4.4.3 Set Date and time .................... 25
4.4.4 Calibrate Dark Current ................. 26
4.4.5 Wavelength Calibration ................ 26
4.4.6 Input the lamps changing Wavelength Point .. 27
4.4.7 Reload Default....................... 26
4.4.8 Version ............................ 28
5 Instrument Maintenance
1. Daily Maintenance .............................. 28
2. Trouble shooting ............................... 29
3. Spare part Replacement .......................... 30
1. Preface
The safety statements in this manual comply
with the requirements of the HEALTH AND
SAFETY AT WORK ACT, 1974.
Read the following before installing and
using the instrument and its accessories.
This instrument should be operated by
appropriate laboratory technicians.
1. Safety
The apparatus described in this manual is
designed to be used by properly trained
personnel in a suitable equipped laboratory.
For the correct and safe use of this
apparatus it is essential that laboratory
personnel follow generally accepted
safe procedures in addition to the safety
precautions called for in this manual.
The covers on this instrument may be
removed for servicing. However, the inside
of the power supply unit is a hazardous area
and its cover should not be removed under
any circumstances. There are no serviceable
components inside this power supply unit.
For this instrument, avoid touching the high
voltage power supply at all times.
Some of the chemicals used in
spectrophotometry are corrosive and/
or inammable and samples may be
radioactive, toxic, or potentially infective.
Care should be taken to follow the normal
laboratory procedures for handling chemicals
and samples.
2. General

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Before switching on the apparatus, make
sure it is set to the voltage of the local
power supply (see Installation).
The power cord shall be inserted in a socket
provided with a protective earth contact.
The protective action must not be negated
by the use of an extension cord without a
protective conductor.
3. Electrical
Any interruption of the protective
conductor inside or outside the apparatus
or disconnection of the protective earth
terminal is likely to make the apparatus
dangerous. Intentional interruption is
prohibited.
Whenever it is likely that the protection has
been impaired, the apparatus shall be made
inoperative and be secured against any
unintended operation.
Note
NEVER touch or handle the power supply on
this instrument due to the high voltage!
The protection is likely to be impaired if, for
example, the apparatus
• Shows visible damage
• Fails to perform the intended
measurements
• Has been subjected to prolonged storage
under unfavorable conditions
• Has been subjected to severe transport
stresses
4. Warning
To ensure that the instrument is working
within its specication, especially when
making measurements of an important
nature, carry out performance checks with
particular reference to wavelength and
absorbance accuracy. Performance checks
are detailed in this manual.
5. Performance
For compliance with the EMC standards
referred to in the EC Declaration of
Conformity, it is necessary that only shielded
cables supplied by us are used when
connecting the instrument to computers and
accessories.
6. Radio Interference

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2. Introduction of the Instrument
1. Brief Introduction of the Instrument This
instrument is a single beam, general purpose
instrument designed to meet the needs of
the Conventional Laboratory, This instrument
is ideal for various applications, such as:
Chemistry, Biochemistry, Petrochemistry,
Environmental Protection, Food and
Beverage Labs, Water and Waste Water
Labs and other elds of quality control and
research.
This instrument incorporates a 128 x 64 dots
matrix LCD display for photometric results,
easy operation and wavelength range of
190 nm to 1100 nm. This instrument is
ideal for measurements in the visible and
ultraviolet wavelength region of the
electromagnetic spectrum.
1. Brief Introduction of the Instrument
Fig Front View
Fig Control Panel

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2. Working Principle
Dierent matter has dierent but special
absorbance wavelength point. Also, when at
the xed wavelength point, the absorbance
has some relation to the substance’s (Always
transparent Solution) concentration and its
thickness. The relation can be concluded
as the following Formula which is called
Lambert-Beer Law.
T= I/Io
A=KCL= -log I/Io
A Absorbance
C Concentration of the Solution
K Absorbance Coecient of the
Solution
L The length of the Solution in
the light path
I The intensity of the light
focused on the A/D after it
permeates the solution to be
measured.
IoThe intensity of the light
focused on the A/D before it
permeates the solution.
Note:
When test, the solvent is usually taken as
the Reference Solution and its Transmittance
is considered as 100 %T. While the
Transmittance of the sample to be tested is a
relative value which is got comparing to that
of the Reference.
Fig Back View Air inlet Fuse Parallel port
USB portPower socketPower switchFan

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3. Structure
The spectrophotometer consists of ve
parts:
1. Halogen or deuterium lamps to supply the
light
2. A Monochromator to isolate the
wavelength of interest and eliminate the
unwanted second order radiation
3. A sample compartment to accommodate
the sample solution
4. Detector to receive the transmitted light
and convert it to an electrical signal; and
A digital display to indicate absorbance or
transmittance. The block diagram below
illustrates the relationship between these
parts.
Fig Block diagram for the Spectrophotometer
Light
Source
Monochromator Sample
Compartment
Detector Process and
Display
In your spectrophotometer, light from the
lamp is focused on the entrance slit of the
monochromator where the collimating mirror
directs the beam onto the grating. The
grating disperses the light beam to produce
the spectrum, a portion of which is focused
on the exit slit of the monochromator by a
collimating mirror. From here the beam is
passed to a sample compartment through
one of the lters, which helps to eliminate
unwanted second order radiation from the
diraction grating. Upon leaving the sample
compartment, the beam is passed to the
silicon photodiode detector and causes the
detector to produce an electrical signal that
is displayed on the digital display.
4. Unpacking Instructions
Carefully unpack the contents and check the
materials against the following packing list to
ensure that you have received everything in
good condition.
Packing List
Description Quantity
Spectrophotometer................................. 1
Power Cord............................................ 1
Glass Cuvettes ........................... 1 Set of 4
Quartz Cuvettes ......................... 1 Set of 2
Operation Manual ................................... 1
Note:
The Printer and Auto-cell Holder mentioned
in this manual are all optional accessories,
they do not come standard with the
instrument.

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5. Specications
Model LLG-uniSpec 2
Wavelength Range 190-1100 nm
Band Width 2 nm
Wavelength Accuracy ± 0.5 nm
Wavelength Repeatability 0.3 nm
Wavelength Setting Auto
Photometric Accuracy ±0.5 % T
Photometric Repeatability 0.3 % T
Photometric Display Range 0-200 % T, -0.3-3.0 A
Stability 0.002 A/h @ 500 nm
Stray Light ≤0.05 % T @ 220 nm, 360 nm
Data Output Port USB
Printer Port Parallel Port
Display 128 x 64 Dots LCD
Lamps Deuterium Lamp & Tungsten Halogen Lamp
Detector Silicon Photodiode
Power Requirement 220 V/50 Hz or 110 V/60 Hz
Dimension (L x W x H) 460 x 380 x 180mm
Net Weight 18 kg

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3. Install Instrument
To ensure the best performance, the
following conditions are required:
• The best working temperature range is
16-30 °C and the humidity is 45-80 %.
• Keep it as far as possible away from the
strong magnetic or electrical elds or any
electrical device that may generate high-
frequency elds.
• Set the unit up in an area that is free
of dust, corrosive gases and strong
vibrations.
• Remove any obstructions or materials
that could hinder the ow of air under and
around the instrument.
• The power requirement is
220±22V@50±1Hz or 110±11V@60±1
Hz.
• Use the appropriate power cord and plug
into a grounded outlet.
• If the local voltage is not stable enough, a
manostat is required.
• Be away from direct sunlight
1. Environment Required
Be sure to set the instrument’s Voltage Switch at your local power
supply, or severe damage may occur! The Voltage Switch is on the
bottom of the instrument.
2. Voltage Conrmation
e.g.: If your local Voltage is 220V, you
should set the Voltage Switch at the
following status.
If your local Voltage is 110V, you should stir
the switch to the Left way by nger, so that
it can be set at 110V.

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Step 1: Check the packing list
Unpack the contents, check the materials
with the Packing List. Any damage or Lost
found, please contact us or the local dealer.
Step 2: Position
Place the instrument on the stable table
carefully.
Step 3: Install printer (Optional)
Make sure the printer is power o; link
the printer’s data cable to the instrument’s
parallel port.
Step 4: Link the power cord
Make sure the instrument’s power switch
is in the O condition, link the power cord
to the instrument and insert another end in
the socket provided with a protective earth
contact.
Step 5: Switch on the power
Check again, make sure that all the links are
right. Switch on the power. The instrument
can be used to measure after self-test and
pre-warm.
3. Installation

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4. Operation Introduction
1.1 Menu Tree
1. Software Structure

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1.2 Firmware Functions
The rmware consists of 4 functions: Basic
Mode, Quantitative, Kinetics and System
Functions.
1.2.1 Basic Mode
Measure the Absorbance, Transmittance or
Energy in a xed wavelength point. The test
result can be stored in the RAM.
1.2.2 Quantitative
Standard Curve Method: Set up standard
curve by standard samples; use the new
curve to measure the unknown sample’s
concentration. The curves and the test
results can be stored in the RAM.
Coecient Method: Input the values
of the curve equation’s coecient, and
then measure the unknown sample’s
concentration.
1.2.3 Kinetics
Measure the absorbance or transmittance
of a sample at a specic wavelength in a
certain time range, the curve can display on
the screen and the data can be printed out.
1.2.4 Setup
Light source management; Get Dark
Current; Calibrate Wavelength
and Set time, etc.
2.1 Choose Photometric Mode
Press Arrow key to choose and
ESC
to
conrm.
2.2 Set Wavelength
In the measurement Interface, press
GOTO λ
to set wavelength, use numeric
keyboard to input the value, press
ESC
to
conrm and set 100%T/0Abs
automatically.
2.3 Set Parameters
Press
SET
to go into the parameter setting
interface, press Arrow Key to choose the
corresponding menu and use numeric key to
input the parameter, press
ESC
to conrm,
press
ESC
to return.
2.4 Set the position of auto-cell holder.
(The Auto Cell Holder is optional)
In the measurement Interface, press
CELL
rst, then press the numeric key of 1-8, and
then the corresponding cell will be positioned
at the light path.
2.5 Delete the Input Value
Press
CLEAR
to delete the last character of
the input.
2.6 Delete the test result and stored
data
In the measurement Interface, press
CLEAR
to delete the test result and stored data.
2.7 Calibrate 100%T/0Abs
Put the reference in the light path, press
ZERO
to calibrate 100%T/0Abs.
2.8 Measure the samples
Put the samples in the light path and press
START
STOP
to measure.
2.9 Print the test result
In the Measurement Interface, press
PRINT
to print the result
2. Basic Operation

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3.1 Switch on the instrument and
begin the system self-test (Diagnostic
Program)
Make sure to remove all the blocks in the
light path; Close the compartment lid and
switch on the power; Then the system will
begin self-test.
Note:
During the Diagnostic Program runs, please
DON’T open the lid of the compartment!
3.2 Pre-warm
When the diagnostic program nished, it
goes into pre-warm condition. 20 minutes is
required before measure.
3.3 Check the Cuvettes
The cuvette should be clear and there’s no
solution remains on the transparent surface.
If the wavelength is below 340 nm, Silicon
Cuvette is a must.
3. Preparation before test
4.1 Basic Mode
Step 1: Go into Basic Mode interface
In the main menu, use Arrow Key to choose “Baisc Mode”, press
ENTER
to go into
corresponding setting interface.
4. Measurement
Basic Mode
Quantitative
Kinetics
Setup
ENTER
ESC
Basic Mode
0.000 Abs
500.0 nm
Step 2: Set Wavelength
Press
GOTO λ
to set Wavelength, input the wavelength value by the numeric Keypad,
then press
ENTER
to conrm.
Basic Mode
0.000 Abs
500.0 nm
ESC
GOTO λ Basic Mode
WL= 500.0 nm
Please Input WL.
WL=

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Step 3: Set Photometric Mode
Press
SET
to go into Parameter Setting Interface, press Array Key to select “Abs”, “%T” or
“Energy” mode, press
ENTER
to conrm. Press
ESC
to return.
Basic Mode
0.000 Abs
500.0 nm
SET Basic Mode
Abs
% T
Energy
ESC
Step 4: Go into Continuous Measurement Interface
Press
START
STOP
to go into continuous measurement interface.
Basic Mode
0.000 Abs
500.0 nm
START
STOP 500.0 nm 0.000 Abs
No. WL. Abs.
ESC
Step 5: Calibrate 100%T/0 Abs
Put the Reference Solution in the light path, press
ZERO
to calibrate 100 %T/0Abs.
Step 6: Measure Sample
Put the unknown concentration sample in the light path, press
START
STOP
to measure, and the
result displays in the data sheet. At the same time, the test result will be stored in the RAM
automatically.
Repeat this step to nish all the samples measurement.
Step 7: Print the data
Press
PRINT
to print the test result.
Step 8: Delete the data
Press
CLEAR
to delete the test data.

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4.2 Quantitative
Step 1: Go into Basic Mode interface
In the main menu, press Arrow Key to choose “Quantitative”; Press
ENTER
to enter the
Quantitative method selecting Interface.
Basic Mode
Quantitative
Kinetics
Setup
ENTER
ESC
Quantitative
Standard Curve
Coecient
Unit
Step 2: Choose Unit
Move the cursor on “Unit”, press
ENTER
to go into concentration unit choosing interface.
Use Arrow Key to move the cursor on the unit you want, and press
ENTER
to conrm.
Quantitative
Standard Curve
Coecient
Unit
ENTER
ESC
Step 3: Choose Methods
There are two methods to be selected: Standard Curve and Coecient.
I. Standard Curve:
(1) Go into “Standard Curve” Interface
In “Quantitative” menu, press Arrow Key to choose “Standard Curve”; Press
ENTER
to go into
sub menu. Here you can create a new curve, or you can load a saved curve. If you want to
delete a saved curve, just choose “Delete Curve”.
Quantitative
Standard Curve
Coecient
Unit
ENTER
ESC
Standard Curve
Create Curve
Load Curve
Delete Curve

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(2) Set Wavelength
Move the cursor on “Create Curve”, then press
ENTER
to go into the Fit Method select
interface. If you select “Linear Zero”, then the curve must go through the zero point. If you
select “Linear Fit”, then it doesn’t necessarily go through zero point.*
Standard Curve
Create Curve
Load Curve
Delete Curve
ENTER
ESC
Fit Method
Linear Fit
Linear Zero
* After Fit method selected, press
ENTER
to go into the following interface.
Fit Method
Linear Fit
Linear Zero
ENTER
ESC
500.0 nm 0.000 Abs
Input Stds No:
No. =
* Press
GOTO λ
to go into the wavelength setting interface, input the WL. Value by the numeric
Keypad and press
ENTER
to conrm.
500.0 nm 0.000 Abs
Input Stds No:
No. =
GOTO λ Quantitative
WL= 500.0 nm
Please Input WL.
WL=
(3) Calibrate 100 %T/0Abs
Put the Reference Solution in the light path, then press
ZERO
to calibrate 100 %T/0Abs.

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(4) Conrm the quantity of standard samples
Use the numeric keypad or Arrow Key to input the quantity of the standard samples (e.g.
There are 6 Standard Samples), then press
ENTER
to conrm. The system will go into the
following interface.
500.0 nm 0.000 Abs
Input Stds No:
No. =
ENTER
500.0 nm 0.000 Abs
Put 1 # Standard,
Input 1 # Conc.:
C =
(5) Measure Standard Samples’ Concentration
* Pull the 1# Standard Sample in the light path and input the value of its concentration by
the numeric keypad, then press
ENTER
to conrm. The system will go into the next interface.
500.0 nm 0.100 Abs
Put 1 # Standard,
Input 1 # Conc.:
C =
ENTER
500.0 nm 0.100 Abs
Put 1 # Standard,
Measure 1 # Abs.
* Press
ENTER
to measure 1# Standard Sample, then it goes into the next Interface.
500.0 nm 0.100 Abs
Put 1 # Standard,
Measure 1 # Abs. ENTER
500.0 nm 0.100 Abs
Put 2 # Standard,
Input 2 # Conc.:
C =
* Pull the 2# Standard Sample in the light path and input its concentration, then press
ENTER
to conrm. Press
ENTER
again to measure 2# sample’s concentration.

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* Measure the other standard samples in the same way. When the last sample is nished,
press
ENTER
to conrm. Then the Standard Curve and its equation will display on the screen
automatically. At the same time, the Curve Equation will be saved in the RAM automatically.
500.0 nm 0.100 Abs
Put 6 # Standard,
Measure 6 # Abs.
ENTER
Note:
If there’s some mistake during the operation, the system will beep 3 times and return the
initial interface automatically. Of course, the curve can not be displayed on the screen.
(6) Measure unknown Samples
* Pull the Reference solution in the light path and press
ENTER
to go into the continuous
measurement interface.
ENTER 500.0 nm 0.100 Abs
No. WL. Abs.
ESC
* Pull the concentration unknown sample in the light path, then press
START
STOP
to measure. The
result will be displayed on the screen one by one. At the same time, the data will be saved in
the RAM automatically. Total 200 groups of data can be saved.
(7) Load Curve
All the curve equations will be saved in the RAM automatically. If you want to reload the
saved curve, just follow the following steps:
* Move the cursor on “Load Curve” and press
ENTER
to conrm. Then the system will go into
the curve equation record interface.
ENTER No. Curve Data
ESC
Standard Curve
Create Curve
Load Curve
Delete Curve

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* Move the cursor on the equation that you need and press
ENTER
to conrm. Then the
corresponding curve will display on the screen.
ENTER
ESC
No. Curve Data
* Press
ENTER
to go into sample continuous measure interface and measure samples.
Note:
When you reload the curves, the wavelength will be set at the point where you create the
Standard Curve automatically.
(8) Delete Curve
* Move the cursor on “Delete Curve” and press
ENTER
to conrm. Then the system goes into
the curve equation choosing interface.
ENTER No. Curve Data
ESC
Standard Curve
Create Curve
Load Curve
Delete Curve
* Move the cursor on the curve equation that you don’t need again and press
ENTER
, then
system will ask you to conrm again.
ENTER
No. Curve Data
ESC
Delete Curve
YES
NO
* Move the cursor on “Yes” and press
ENTER
to conrm, then the curve equation will be
deleted. If you don’t want to delete it, just choose “NO” or press
ESC
to return.

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II. Coecient Method:
(1) Go into “Coecient” method.
Press Arrow Key to choose “Coecient”, press
ENTER
to go into pre-test interface, then press
SET
to go into the parameter setting interface.
ENTER
ESC
Standard Curve
Create Curve
Load Curve
Delete Curve
Coecient Standard Curve
Coecient K
Coecient B
(2) Parameter Setting
* Use Arrow Key to move the cursor on “Coecient K” and press
ENTER
to go into K setting
interface. Input K and press
ENTER
to conrm, the system will return the former interface
automatically.
ENTER Coecient K
ESC
Coecient
Coecient K
Coecient B
* Input the value of B in the same way, press
ENTER
to go back to the pre-test interface.
(3) Set Wavelength
Press
GOTO λ
to go into wavelength setting interface, input the wavelength value by the
numeric keypad and press
ENTER
to conrm.
ENTER
ESC
Coecient Quantitative
WL= 500.0 nm
Please Input WL.
WL=