Llutico N125-DE User manual

Biological Microscope
Model Number: N125-DE
User Manual
Store Name：LluticoDE
Contact：[phone]
Email：[email protected]
This manual is for biological microscope Model N125-DE.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope,
it is recommended strongly that you study this manual thoroughly before operating the microscope.

Contents N125-DE
Contents ............................................................................................................
User Notices .....................................................................................................
1. Components Name...................................................................................
2. Assembly ....................................................................................................
2.1 Assembly Diagram .................................................................................
2.2 Assembly procedure ............................................................................
3. Adjustment and Operation......................................................................
3.1 Adjustment set diagram .......................................................................
3.2 Adjustment and Operation..................................................................
4. Technical Specifications..........................................................................
4.1 Main specifications .................................................................................
4.2 Eyepieces and Objectives ....................................................................
5. Outfit ..............................................................................................................
6. TroubleShooting Guide ..........................................................................
7. Warranty .......................................................................................................
1
2
3
4
4
5
9
9
11
13
13
13
14
15
17

Use Notices N125-DE
Ⅰ. Safety note
1. Open the box carefully to avoid the accessories, like lens, dropping to ground or being damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dusty and easy shaking
environment. Make sure the stage is flat, horizontal and firm enough.
3. When moving the microscope, carefully carry it with the handle and the base.
4. Make sure the instrument is earthed, toavoid lighting strike.
5. For safety, be sure the main switch is in “O” (off) state and cut off the power supply before replacing
the bulb or the fuse. If you replace the bulb during use or right after use, allow the lamp bulb and
the lamp house to cool completely before touching.
(Designated bulb：1W S-LED or 6V20W Halogen Lamp)
6. Check the input voltage: be sure the input voltage which is signed in the back of the microscope is
consistent with the power supply voltage, or it will bring a serious damage to the instrument.
7. Always use the power cord provided by Novel.
8. The electrical equipment of the microscope should be discard as electronic waste.
Ⅱ. Maintenance and Care
1. All the lenses have been adjusted properly; do not dismount them by yourself please.
2. The nosepiece and coarse and fine focusing parts are so delicate that it is forbidden to disassemble
them carelessly by yourself.
3. Keep the instrument clean, and do not pollute the optical element when wiping away the dust on
the instrument.
4. The contaminations on the prism, like fingerprints and oil smudges, could be gently wiped with a
piece of soft cloth or tissue paper, gauze which has been immersed in pure alcohol or ether. (Note
that the alcohol and ether are highly flammable, do keep them away from the fire or potential
sources of electrical sparks, and use them in a drafty room as possible as you can.)
5. Do not attempt to use organic solvents to clean the microscope components other than the glass
components. To clean them, use a lint-free, softcloth slightly moistened with a diluted neutral
detergent.
6. When using, if the microscope is splashed by liquid, cut off the power at once, and wipe away the
splash.
7. Do not disassemble any parts of the microscope, as this will affect the function or reduce the
performance of the microscope.
8. Place the instrument in a cool, dry position. When not using the microscope, keep it covered with a
dust cover. Make sure the lamp socket is cool before covering the microscope.
2

1. Components Name N125-DE
Eyepiece Binocular viewing head
Nosepiece
Objective
Illumination
Main body
Condenser
Biological Microscope N125-DE
3
Stage

2.1 Assembly Diagram
2．Assembly N125-DE
4
The following figure shows the installation sequence of the components. The number in the figure
shows the assembly steps.
★ Before installing, be sure every components is clean, do not score any parts or glass surface.
★ Keep well with hexagon wrench provided. When changing the components, you will need it
again.
④
③
①
②
10X Eyepiece
20X Eyepiece
Objective
Main body Power cord
Binocular Viewing Head
WF20X WF20X

2.2 Assembly procedure
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5
Fig.1
Fig.2
Fig.3
Fig.4
2.2.1 Installing binocular viewing head（Fig.1-2）
Insert the binocular viewing head into the microscope
head and turn it to a proper position, then tight it.
Insert the eyepiece into the eyepiece tube until they
are against each other, as shown in Fig.4.
2.2.2 Installing the eyepiece （Fig3-4）

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6
Fig.5
Fig.6
Fig.7
2.2.3 Installing the objective（Fig.5-6）
1. Adjusting the coarse focus knob until the support
device of the mechanical stage reaches its low limit
position.
2. Screw the lowest magnification objective into the
nosepiece from the left or the right side, then
revolve the nosepiece clockwise and mount other
objectives by the sequence of low to high
magnification
◇ Installing objective this way will make the change of
magnification to be easier during using.
★ Clean the objective regularly, for lens is
susceptible to dust.
★ When operating, use 10 × magnification
objective to search and focus specimen
firstly, then replace with higher
magnification objective if necessary.
★ When replacing the objective, slowly turn
the nosepiece until you hear “clicked”,
that means the objective is in place.
2.2.4 Mounting the filters（Fig.7）
1. Open the condenser carriage①；
2. Place the required filter②into the condenser ，
then close the condenser carriage。
★ the filter of the standard outfit is green and
baby blue.
①
②

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7
Fig.8
Fig.9
Fig.10
2.2.5 Connecting the Power Cord (Fig.8-10)
★ The cable and cords are vulnerable when
bent or twisted, never subject the power cord
to excessive force.
1. Set the main switch①o “O” (off) state before
connecting the power cord.
2. Insert the plugs②into the power jack③f the
microscope safely.
3. Plug the power cord④into the power supply
receptacle⑤. Make sure the connection is well.
★ Do use the supplied power cord all the time.
If lost or damaged, select the same standard
cord, please.
★ A wide range of voltage ,like 100V～240V,
is acceptable for this microscope.
2.2.6 Replacing the fuse（Fig.9-10）
Do remember to set the main switch①to the state “O”
(OFF) and unplug the power cord before replacing the
fuse. Rotate the fuseout of the holder ③ , replace with
a new fuse, then rotate it back to the holder again.
④ ⑤
③
①
②

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8
Fig.11
Fig.12
2.2.7 Installing and Replacing the bulb
（Fig.11-12）
◇ Please use the specified halogen Lamp 6V20W.
1. Loosing the screw①。
2. Hold the bulb②after you wrap it with gauze or
other protection materials, and then insert its
pindeeply intothe jack in the lamp holder.
3. Duringmicroscope using or soon after it is turned
off, the bulb, the lamp house and nearby parts will
be very hot and will cause serious burns. Please
turn the main switch to“O” (off) and disconnect the
power plug, and make sure the bulb, the lamp
room and periphery are all cool. Then, you can do
your replacing.
★ Insert the bulb gently. Squeezing too hard
will damage the bulb.
★ To prevent reduced bulb life or cracking, do
not touch the bulb with bare hands. If
fingerprints are accidentally left on the bulb,
wipe the bulb with a soft cloth.
②
①

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9
Fig.13
3. Adjustment and Operation
3.1 Adjustment set diagram
Portrait Adjustment Knob
Right Coarse Focus Knob
Lateral Adjustment Knob
Aperture Iris Diaphragm adjustment
Main Switch
Tension Adjustment Collar Right Fine Focus Knob

Fig.14
Interpupillar DistanceIndicator
Condenser Focusing Knob
Brightness Adjustment Knob
N125-DE
10
Clocking Set
Left Coarse Focus
Left Fine Focus Knob

Fig.15
Fig.16
Fig.17
Fig.18
N125-DE
10
3.2 Adjustment and Operation
3.2.1 Brightness Adjustment（Fig.15-16）
1. Connect the power cord and set the main switch①
to “－”state (ON).
2. Turning the brightness adjustment knob ②.
3.2.2 Placing the Specimen (Fig.17)
1. Place the specimen the center of the mechanical
stage and use the stage clips to clamp it.
2. Turn the portrait and lateral adjustment knob① of
the mechanical ruler, move the specimen to the
required position.
★ Be careful when changing the objective. If
you finish the observation with the short
working distance objective, and want to
change another one, becareful of not letting
the objective touch the specimen.
3.2.3 Focusing the Specimen（Fig.18）
1. Focus the specimen with 10X objective. To avoid the
objective touching the specimen during focusing,
you should raise the mechanical stage to let the
specimen close to the objective at first, then slowly
separate them to bring the specimen to focus.
★ To make the observation more convenient,
you can use the locking set③to fix the stage
in a vertical direction.
①
①
②
①
②③
Turn the coarse focus knob①conversely to lower the
specimen and search images in the 10×ocular
simultaneously, and thenuse the fine knob② to focus.
After that, you can replace with other magnification
objectives safely, and focus without the risk of
damaging the specimen.

Fig.19
Fig.20
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11
3.2.4 Condenser Adjustment（Fig.19）
Turn the condenser focus knob①to move the
condenser up and down.Raise the condenser when
using the high magnification objective, and descend
it when using the low magnification one.
★ The condenser and the objectiveare coaxial.
It has been adjusted before leaving factory,
so the user needn’t to adjust them by self
★ The highest position of the condenser has
been adjusted too. It also needn’t any user’
s operation. (The top surface of the condenser
is 0.03mm-0.4mm lower than the stage top
surface.)
★ The aperture iris diaphragm is designed for
the adjustment of the numerical aperture,not
for the brightness.
Generally, setting the aperture iris diaphragm
to 70- 80% of the N.A. of the objective in use will
provide an image with good contrast.If you want
to observe the image of the aperture iris diaphragm,
remove one eyepieceand look through the tube.
You will see a dark circle encroaching on the
bottom of the tube.
3.2.5 Aperture Iris Diaphragm Adjustment
（Fig.20）
Turn the aperture iris diaphragm stick①to adjust the
aperture iris diaphragm.
②
①
①
1. Focus the specimen with 10× objective.
2. Adjust the condenser focus knob①to get a clear
image of the field iris diaphragm.
3. Turn the condenser centering knobs②to center the
image of field iris diaphragm in the field of view.

Fig.21
Fig.22
Fig.23
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3.2.6 Adjusting the Interpupillary Distance（Fig.21）
3.2.7 Adjusting the Diopter (Fig.22)
3.2.8 Adjusting the tension adjustment collar
（Fig.23）
The interpupillary distance range: 48mm-75mm。
While looking through the eyepieces, move both
eyepieces round until the left and right fields of view
coincide completely.
1. Turn the eyepiece①to adjust the diopter while
looking through it.
★ The diopter range of the eyepiece is±5 diopter.
The number aligned to the line on the viewing
head is the diopter in use.
①
① ② ③
★ Turn the tension adjustment collar①with your
fingers.When the collar is turned in the direction
of the arrow, thetension of the coarse adjustment
knob② increases. Turning the collar in the opposite
direction decreases the tension.
If the nosepiece descends on its own or if the
specimen gets out of focus quickly even when it is
brought into focus using the fine adjustment
knob③, it means the tension of the coarse
adjustment knob is too low. Turn the collar in the
direction of the arrow to increase the tension.

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4．Technical Specifications
4.1 Main specifications
Optical System
Viewing Head
Eyepiece
Nosepiece
Objective
Focusing
Condenser
Stage
Illumination
Seidentopf Binocular Head, 30°Inclined,Interpupillary Distance 48-75 mm
WF 10X / WF 20X
Limited optical system/Infinite optical system
Backward Quadruple Nosepiece
Achromatic Objective 4×，10×，40×、100×
Coaxial Coarse and Fine Adjustment, Moving Range 0.002mm，Fine Division 20mm
Abbe Condenser,NA1.25
Double Layers Mechanical Stage160×140，Moving Range74×50
1W S-LED /6V20W Halogen Lamp
4.2 Eyepieces and Objectives
4.2.1. Achromatic Objective
Magnification Numerical
Ap erture（NA） Focal Length(mm) Working Length
（mm）
Focal distance
（mm） Objective
4× 0.10 0.17 31.05 37.5 Dry
10× 0.25 0.17 17.13 7.316 Dry
40× 0.65 0.17 4.65 0.632 Dry
100× 1.25 0.17 2.906 0.198 Oil
4.2.2. Eyepiece
Eyepiece Magnification Focal Length （mm） Field of View（mm）
Wide field eyepiece 10× 24.95 Φ18
Wide field eyepiece 20× 12.5 Φ12
4.2.3. Total Magnification
Eyepiece
Objective
Total Magnification
20×
10×
4×
40×
40×
400×
100×
1000×
10×
100×
4×
80×
40×
800×
100×
2000×
10×
200×

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5．Outfit
Item Specification Quantity Standard Outfit
Main Body
Viewing Head
Condenser
Nosepiece
Illumination
Eyepieces
Main Standard
Main Standard（battery charging）
Double Layers Mechanical Stage
Condenser Holder
Seidentopf Binocular Head
Seidentopf Trinocular Head
Abbe Condenser, NA=1.25
Backward Quadruple Nosepiece
1W SLED
6V20WHalogen Lamp
Blue, GreenFilter
Spare Fuse 50T250V 500mA
10×Wide Field Plan Eyepiece
20×Wide Field Plan Eyepiece
Achromatic Objective4×
Achromatic Objective10×
Achromatic Objective40×
Achromatic Objective100×（Oil, Spring）
1
1
1
1
1
1
1
1
1
1
1 each
2
2
2
1
1
1
1
●
○
●
●
●
○
●
●
●
○
○
●
●
●
●
●
●
●
Objectives
Note：“●”Standard Outfit, “○”optional

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6．Troubleshooting Guide
TROUBLE
1. The edge of the field of
view is dark or the
brightness is not uniform
2. Dirt or dust is visible in
the field of view
3. Visibility is poor Image
is not sharp; Contrast is
poor; Details are indistinct
The nosepiece is not in the located
position (objective and light path not coaxial)
The surface of the lamp becomes black Change a new lamp bulb
A lens (the objective,
condenser, eyepiece or collector) is dirty.
Locate the nosepiece
properly where it clicks
Clean it thoroughly
4. One side of image
is blurred
5. The brightness is not
enough
CAUSE SOLUTION
Dirt/dust on the specimen Replace with a clean specimen
Dirt/dust on the eyepieces
Specimen is not covered Add cover glass on it
Clean them
The thickness of the cover glass is not suitable
Specimen is placed reversely
Dry objective has oil on it.
(especially for 40X objectives)
A lens (the objective, condenser,
eyepiece or collector) is dirty.
Immersion oil is not used with the 100x objective
Air bubbles existed in the immersion oil
The aperture iris diaphragm is stopped
down too far
Dirt or dust on the eyepiece
Condenser is not properly centered
The nosepiece is not properly engaged
The specimen is not clamped
The aperture iris diaphragm is too small
The condenser is too low
A lens (the objective, condenser,
eyepiece or collector) is dirty
Use standard cover glass
with thickness of 0.17mm
Turn it over
Wipe the oil
Clean it
Use specified oil
Eliminate the bubble
Adjust the aperture iris
diaphragm properly
Clean it
Center the condenser with the
centering screw
Engage the nosepiece properly
Clamp it with stage clips
Adjust it properly
Adjust it properly
Clean it
6.1 Optical System

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6.2 Mechanical System
6.3 Electrical System
TROUBLE CAUSE SOLUTION
TROUBLE CAUSE SOLUTION
Interpupillary distance is incorrect Adjust interpupillary distance
1.Field of view of one eye does not
match that of the other
2. Observation is tiring
The diopter is not proper
The brightness of the illumination
is not proper for eyes
Adjust the diopter properly
Adjust the lamp voltage
1. The bulb cannot light
2. The bulb burns out suddenly
3. the illumination is not bright enough
4. Image flicks
No power supply
The pin of the bulb doesn’t insert
properly
The bulb broken
The bulb is not the specified one;
The voltage is too high
the voltage is too low
The bulb is about to burn out
The bulb is not inserted deeply
Check the power cord connection
Insert the pin deeply
Replace with a new one
Use the specified bulb;
lower the voltage
Raise the voltage
Replace with a new one
Check its connection

N125-DE
You May Return Your Product For A Full Refund Within The First 90 Days Of Your Purchase.
If you encounter any quality problem with this product within one year after using it, please contact us.
We will be able to repair or replace parts for free. Even more than a year, we will provide replacement
parts at low prices continuously.
1-year parts warranty
Returning The Product
90-Day Money Back
7. Warranty
Contact Us At [email protected] For The Return Address, We Will Issue Replace/Refund Upon
Receiving Your Return.
Customer Service E-mail：[e-mail]