MagCore HF48 User manual

Operation Manual
Ver. 20220214
www.rbcbioscience.com
CONTENTS
Safety Precautions . . . . . . . . . . . . . . . . . . . 1
Specification . . . . . . . . . . . . . . . . . . . . . . . . 2
━ Operation Parameters . . . . . . . . . . . . . . . . . . . 2
━ Operation Environment . . . . . . . . . . . . . . . . . . 2
━ Applications . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Accessories . . . . . . . . . . . . . . . . . . . . . . . . 3
System Overview . . . . . . . . . . . . . . . . . . . . 4
Installation . . . . . . . . . . . . . . . . . . . . . . . . . 5
━ Before Installation . . . . . . . . . . . . . . . . . . . . . . 5
━ Install MagCore . . . . . . . . . . . . . . . . . . . . . . . 6
━ Install Tip and Sample Tube . . . . . . . . . . . . . . . . 7
━ Install Elution Tube . . . . . . . . . . . . . . . . . . . . . . 7
━ Install Cartridge . . . . . . . . . . . . . . . . . . . . . . . . 7
Touch Panel and User Interface . . . . . . . . . 8
━ Main Menu . . . . . . . . . . . . . . . . . . . . . . . . . . 8
━ Panel Functional Area . . . . . . . . . . . . . . . . . . . . 8
Emergency Stop and Warning . . . . . . . . . . 9
━ Start . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
Load Program . . . . . . . . . . . . . . . . . . . . . 11
━ Save Programs . . . . . . . . . . . . . . . . . . . . . . . .12
━ Delete Programs . . . . . . . . . . . . . . . . . . . . . . .12
Update Program Files . . . . . . . . . . . . . . . . . . . . .13
Easy Maintenance . . . . . . . . . . . . . . . . . . 13
━ Replace O-Rings . . . . . . . . . . . . . . . . . . . . . . .14
━ Clean Piercing Needles . . . . . . . . . . . . . . . . . . .14
━ Piston Test . . . . . . . . . . . . . . . . . . . . . . . . . . .15
━ Rejection Tip . . . . . . . . . . . . . . . . . . . . . . . . .16
━ Heater Test . . . . . . . . . . . . . . . . . . . . . . . . . .16
━ UV Sterilization . . . . . . . . . . . . . . . . . . . . . . . .17
━ Replace UV Lamp . . . . . . . . . . . . . . . . . . . . . .17
━ Moving Test . . . . . . . . . . . . . . . . . . . . . . . . . .18
━ DC Motor Operate . . . . . . . . . . . . . . . . . . . . .18
━ System Timing Setting . . . . . . . . . . . . . . . . . . . . .19
Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
System Status . . . . . . . . . . . . . . . . . . . . . . . . 20
━ Export Error Record File . . . . . . . . . . . . . . . . . . . . .20
Others . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
━ Replace Fuses. . . . . . . . . . . . . . . . . . . . . . . . . . .21
━ Barcode Scan . . . . . . . . . . . . . . . . . . . . . . . . . . .21
Troubleshoots . . . . . . . . . . . . . . . . . . . . . . . 22

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Safety Precautions
Handle the power cord carefully.
Hold the plug when unplugging the cord.
Keep the set free from moisture,
liquid, and dust.
Unplug the power cord if the system
will not be used again in a long time.
Do not obstruct the ventilation space.
Do not let foreign objects in the set.
Do not let insecticides, benzene, or
thinner come in contact with the set.
Do not ever disassemble or modify
the set in any way.
Avoid high temperatures.
Leave space for sufficient heat dissipation during installation.
CAUTIONS
Risk Of Electric Shock
Do Not Open
Before use
WARNINGS and CAUTIONS stated below are intended to protect the individual from injuries
and avoid damage to machines.
CAUTION:
To reduce the risk of electric shock, do not remove the cover. Refer to qualified
service technician for inner part servicing.
CAUTION:
1. Handle the power supply cord carefully
Do not damage or twist the power supply cord. Damaged or twisted cord may
cause electric shock or error when used. When you remove it from the outlet, be
sure to remove by holding the plug and not by pulling the cord.
2. Do not open the top cover
In order to prevent electric shock, do not open the top cover.
3. Do not place anything inside
Do not place metal objects inside the MagCore® System and wipe away all spilled
liquid to minimize the risk of system error and electric shock.
Note On Use:
1. Read Instructions – All the safety and operating instructions should be read
before operating the instrument.
2. Keep the Instructions – Please keep the safety and operating instruction
manual for future reference.
3. Cautions and Warnings – All warning signs should be attached on the
product and shown in the user's manual.
4. Follow Instructions – All operating and use instructions should be followed.
5. Cleaning – Unplug the product before cleaning. Use only 75% of EtOH to
clean the surface of instrument.
6. Attachments – Do not attach anything on the instrument.
7. Liquid and Moisture – Do not use this product near water and avoid moist
areas - bath tub, wash bowl, kitchen sink, laundry, swimming pool or in a moist
basement.
8. Proper Installation – Do not place this product on an unstable cart, stand,
tripod, bracket, or table. The product may fall and caues serious injury to a child
or adult, and serious damage to the product. Use only with a cart, stand, tripod,
bracket, or table recommended by the manufacturer, or sold with the product.
Any mounting of the product should follow the manufacturer’s instructions,
and should use a mounting tool recommended by the manufacturer.
9. Ventilation – Slots and openings in the cabinet are designed for ventilation,
which is required for safe operation and minimizing the chance of overheating.
The product should be placed on a hard surface to ensure proper ventilation.
This product should not be placed in a built-in installation such as a bookcase
or rack unless proper ventilation is ensured or instructed by the manufacturer.
10. Power Sources – This product should be operated only from the type of power
source indicated on the label. If you are not sure of the type of power supply at
your lab, consult the seller or local power company. For products intended to
operate from battery or other sources, refer to the operating instructions.
11. Grounding or Polarization – This product may be equipped with a polarized
alternating-current plug. This plug will fit into the power outlet in one way only.
This is a safety feature. If you are unable to insert the plug fully into the outlet,
try reversing the plug. If the plug should still fail to fit, contact your electrician to
replace your obsolete outlet. Do not defeat the safety purpose of the polarized
plug.
12. Power-Cord Protection – Power-supply cords should be routed so that they
are not likely to be walked on or pinched by items placed near or against them.
Pay extra attention to the plugs, convenience receptacles, and the connecting
point where the cord extends out of the product.
13. Lightning – Unplug the product if it will not be used again for a long period or
in the case of a heavy storm. This will prevent damage to the product due to
lightning and power surges.
14. Overloading – Do not overload wall outlets, extension cords, or integral
convenience receptacles as this can result in a risk of fire or electric shock.
15. Object and Liquid Entry – Never push objects of any kind into this product as
they may touch dangerous voltage points or short-out parts that could result
in a fire or electric shock. Never spill liquid of any kind on the product.
16. Servicing – Do not attempt to repair this product by yourself. Refer all servicing
to qualified service technician.
17. Damage Requiring Service – Unplug this product from the wall outlet and
refer servicing to qualified service technician under the following conditions:
a) When the power-supply cord or plug is damaged,
b) If liquid has been spilled, or objects have fallen into the product,
c) If the product has been exposed to rain or water,
d) If the product does not operate normally following the operating
instructions. Adjust only controls covered by the operating instructions as an
improper adjustment of other controls may result in damage and will often
require extensive work by a qualified technician to restore the product to its
normal operation,
e) If the product has been dropped or damaged in any way, and
f) When the product exhibits a distinct change in performance
– this indicates a need for service.
18. Replace Parts – When replacement is required, be sure the service technician
uses replacement parts specified by the manufacturer or those with similar
features as the original part. Unauthorized substitutes may result in fire, electric
shock, or other hazards.
19. Safety Check – Upon completion of any service or repairs to this product, ask
the service technician to perform safety checks to confirm that the product is in
proper operating condition.
20. Heat – The product should be situated away from heat sources such as
radiators, heat registers, stoves, or other products that produce heat.
General Warning
Warning: Biological Hazard
Warning: High Temperature
Warning: Electricity
SAFETY INSTRUCTIONS

2
MagCore® HF48
Model HF48 / MCA4801 (under development)
Processing Capacity Max .48 samples per batch
System Feature 1. Cartridge rack, T-Rack, Tube Rack designed for easy installation
2. Auto-cartridges locking
Power Supply Voltage: AC 200V-220V; Frequency: 50/60Hz;
Dimension W1000 x D800 x H1600 (mm)
W39.37 X D31.49X H62.99(inches)
Net Weight 250kg / 551.25Lb
Specification
━ Operation Parameters
━ Operation Environment
━ Applications
Whole Blood DNA, Viral Nucleic Acid (DNA/RNA 200μl/400μl/1200μl), Plasma and Circulating
Cell-Free DNA (1200μl), Tissue Genomic DNA, Plant Genomic DNA, Bacterial DNA, Total RNA from
Whole Blood and Total RNA from Cultured Cells.
Processing Capacity 1~48 samples per batch
Sample Volume 60/100/150/200 μl /1200 μl
Elution Volume 60/100/150/200 μl
Purity O.D. 1.8 -2.0 A260/280 ratio
Temperatures allowed during transportation, storage, and packaging 15° C - 35° C
Temperatures allowed during operation 18° C - 30o C
Pollution Degree Level 2

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Accessories
Cartridge Rack X 6
Cartridge X 20 Sample Tube X 50 Tip / Tip Holder Set X 50
Elution Carrier X 1
Cartridge Carrier B X 1 / Cartridge Carrier A X 1
Tip Carrier B X 1 / Tip Carrier A X 1
Power Cord X 1 Lubricant X 1 O-Ring X 24
System test tools should be kept in clean places and the loss should be avoided.
System Test Tools
MagCore® HF48 Totle Weight 250kg / 551.25Lb

4
MagCore® HF48
Piston Module
Operating Area B
Storage Cabinet and Electrical Box
Operating Area A
Elution Area
Shutter
Leveling Glides (four legs)
Fixed Knob on the Top Cover of Part Chamber
System Overview
Cooling Fans
Power Switch and Plug
Power Switch (red)
Light Switch (green)
Part Chamber
Maintenance Cover
Status Indicator
Viewing Window
Touch Screen Monitor
Door of Viewing Window
Feeding Door
Top Cover of Part Chamber

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━ Before Installation
Installation
Please find a location close to outlets and leave sufficient space for system
installation and heat dissipation.
Pleaes use the leveling glides to fine-tune the elevation level.
!
System Height
160 cm
System Depth
80 cm
System Width
100 cm
Distance from Wall (left/right)
20 cm Distance from Wall (back)
40 cm

6
MagCore® HF48
━ Install MagCore
Please make sure that the voltage
of the outlet is between 220V and
240V. Keep your hands dry. Please
also make sure that the machine is
turned off when you insert the plug.
Please note the weight of the top
cover of part chamber. Two people
are required to lift it up.
Place the system in a suitable location and use the leveling glides to
fine-tune the elevation. Remember to tighten the screws.
Cut the fixation belt and close the top cover of part chamber.
Cut the fixation belts on the racks.
Plug in the power cord and turn on the main power.
Turn on the power and the system will start initializing setup
automatically.
Please set the local time (refer to
page 19) for the first installation, and
perform the “Piston Test” (page
15).
Please finish reading the operation
manual before operating.
Rotate and pull out the knobs on both sides of the top cover of part
chamber using coins.
Open the top cover of part chamber.
1 2
!
Next Step
Next Step
Next Step
Next Step
Next Step
Next Step

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━ Install Elution Tube
Warning：
DO NOT use other types of centrifuge tubes to replace the Sample Tubes provided.
Poor extraction results or error may occur due to tubes having different heights.
Warning：
Carrier of Operating Area A can only
be used in area A; Carrier of area B
can only be used in area B. Incorrect
use may cause system error.
━ Install Cartridge━ Install Tip and Sample Tube
Insert Tips and Sample Tubes into the slots in the Tip Carrier. Then
place the Tip Carriers into Operating Area A and B as illustrated.
Insert Cartridge into Cartridge Rack.Then place the entire set
of Cartridge Carrier in the slots in Operating Area A and B as
illustrated.
Place Elution Tubes into their corresponding positions and place
Elution Carrier in the elution slot.

8
MagCore® HF48
━ Panel
━ Main Menu
Touch Panel and User Interface
MagCore® HF48 provides 8.9-inch full-color touch screen
and user-friendly interface. You can monitor the progress of
extractions with the status and time displayed on the screen.
Start
Set up basic parameters and operation
Load Program
Load frequently-used programs stored by user
Maintenance and other test functions
Basic maintenance, UV sterilization, and system tests
System time
System status
Basic system information, serial number, error message
Setup
System time setup
Not detected
Normal
Front Door
Front Door is open Not Detected Not Detected Not Detected Not Detected Not Detected
Cartridge Carrier B Tip Carrier B Cartridge Carrier A Tip Carrier A Elution Carrier
I n f o r m a t i o n
Menu
Operating Functions
Message

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Emergency Stop and Warning
Please read about how to stop the system in case of emergency.
Method 1 Method 2 Resume
The system will stop all operations
immediately if the front door is opened.
Select Continue to resume to previous
operations or Abort to end all operations.
Press Stop key to stop all operations immediately
in case of emergency.
Warning：
Please do not place your hands near the operation areas when the
system is under operation for safety reasons.

10
MagCore® HF48
Press Start in the main window.
Select 24 or 48 test channels.
Select Cartridge code.
Please reconfirm the code you selected and input all parameters.
Confirm that the icon does not appear in the status bar. If
the icon shows, operation is disabled. Please solve the error to
continue. (Refer to page 8 for Warning Signs)
After you finish inputting and press Start, a confirmation window
will show up. Please press Yes to operate the system if the
parameters are correct. Please press No to modify incorrect
parameters.
You may monitor the extraction progress and check the remaining
time on the screen. The system will display Complete page after
completion.
━ Start
Please select Channel
Press START to Proceed
105 Plasma DNA Extraction Kit (1.2 ml)
200 µl
150 µl
Remaining time for system operation
Heating Status
Current Operation Status
Parameters
Emergency Stop
Next Step
Next Step
Next Step
Next Step
Please do not open the viewing window
or feeding door during operation if there
is no emergency to prevent error or poor
extraction results.

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Please select one of your saved programs.
Check whether the parameters are correct and press Yes.
Please select Cartridge Code
Please select Channel
Next Step
Next Step
Load Program
You can save frequently used programs with names you prefer for your
convenience.
Press Load Program to enter the Stored Programs page.
Or
Press Load Program after pressing Start to enter the Stored
Programs page.

12
MagCore® HF48
You may save up to 8 programs with names you prefer for your
convenience.
Please press Save key to enter Stored Programs page after all
parameters have been selected on Start page.
Please click to modify.
Confirm if the parameters are correct.
Enter the name you want to save and press Enter.
━ Save Programs
Please select Channel
You may delete your saved program as well.
Please press Load Programs to enter Stored Programs page after
entering Start page.
Please select an item you want to delete by pressing X.
Press Yes to delete the program.
━ Delete Programs
Please select Channel
Next Step Next Step
Next Step
Next Step
Next Step

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Copy new programs into your USB Flash Drive.
* Confirm if "48ExtractorUpdateProgram" is successfully saved in
your USB Flash Drive.
Open the door of viewing window, insert your USB Flash Drive into
the USB slot on the back of the control panel, and press Export key
to export data to your USB Flash Drive.
Select Update Program Files.
Press Yes to start the update and the window will close
automatically after the update is complete.
Easy maintenance includes the mainenance items which can be done by
users. These items include sterilization, cleaning, system tests, error message
report, etc. Users must perform these items regularly to ensure that the system
can function normally.
Update Program Files
UV Sterilization
UV Sterilization
Piston Test
Piston draining and leakage test
Moving Test
Moving Test for all axes
Heater Test
Temperature test for heater
Rejection Tip
Return tips to original positions
Update Program Files
Update programs
O-Ring and Piercing Needles
Replace O-Ring and clean piercing needles
DC Motor Operate
Raise or lower the protective cover on Elution
area and motor in operating area A and B
Easy Maintenance
Please DO NOT modify anything in
48ExtractorUpdateProgram.

14
MagCore® HF48
Select Replace O-Rings and press START.
Follow the instructions: use a sharp blade to cut off old O-rings.
Then press Next.
Apply new O-rings.
Press Finish after applying lubricant.
━ Replace O-Rings
Select Clean Piercing Needles and press START.
Please prepare a cotton paper with 70% alcohol to clean.
━ Clean Piercing Needles
Next Step
Next Step
Next Step
Next Step
Please DO NOT USE other
detergents except 70% alcohol
to clean. Piercing needles are
very sharp, please be careful
not to cut yourself.

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Select Piston test in the Maintenance menu.
Please place the “system test tool”in advance in operating areas
(refer to page 7), and put 1ml water in all Sample Tubes. Press Start
to begin the test.
The purpose of this step is mainly to test whether the instrument
can withdraw tips smoothly.
The display shows Piston Test Step 1, please note that the
illustration shown on screen is only an example. Please check if the
instrument can withdraw tips smoothly. If yes, press Next. If you
find any tip stuck in its tip holder, press Abort to stop the test and
replace the tip/tip holder. Run the test again and if the problem
persists, contact your local distributor for technical support.
The purpose of this step is to test if there is any leakage from the
tips.
The system will absorb water from Sample Tubes. Please check
if the liquid level is the same across all tips. If yes, press Finish. If
the liquid level is not the same throughout, please press Finish
first, then replace the O-ring of the leaking tip and test again. If
the problem persists, please contact your local distributor for
technical support.
━ Piston Test
Next Step
Next Step
Next Step

16
MagCore® HF48
Please place a cartridge into each Cartridge Rack and add 500µl of
water into heat block well 1. Insert a thermometer into heat block
well 1 to measure the temperature.
Select Heater Test in the Maintenance menu.
Press Start. The screen will show the supposed heating temperature
by calculation. Please compare with the value on your thermometer
to see if they are the same.
Please press Stop if you want to stop the test.
━ Heater Test
This function is for the system to return tips to their original
locations after emergency stop or operation interruption due to
power outrage.
Select Rejection Tip in the Maintenance menu
Please select Start to begin. After the process is completed, the
system will return to initial position automatically.
━ Rejection Tip
Next Step
Next Step
heat block well 1
The acceptable range of temperature
is between 60° C and 70° C.
!

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Select UV Sterilization in the Maintenance menu.
You may select to run sterilization for 30min or 60min. Press START.
The screen will show the remaining time.
Please press Stop to stop if you want to stop sterilization.
━ UV Sterilization
Open the top cover of the Part Chamber.
Replace the old UV lamp with a new one.
Select Status in the main menu and press UV Time Reset key to
reset the hours of UV lamp.
Please test to see if the UV lamp is normal after replacement.
━ Replace UV Lamp
Next Step
Next Step
Next Step
Please note that due its weight,
the top cover of the part
chamber requires two people
to lift it.

18
MagCore® HF48
Select Moving Test in Maintenance menu.
Select the axis to test and press Start. The motor will run
continuously until you press Stop.
Select DC Motor Operate in Maintenance menu.
All consumables are fixed in place when test is stopped. You can
operate the DC motor through this function if you need to raise or
lower the drip pan.
Refer to the page 7 for corresponding locations of operation areas.
Select the area you want to move up or down and press Start. It will
stop automatically when the motor moves to end.
━ Moving Test ━ DC Motor Operate
Next Step Next Step
X axis motor running test Operation Area A UP
Shutter Open
Operation Area B UP
Y axis motor running test Operation Area A DOWN
Shutter Close
Operation Area B DOWN
M axis motor running test
V axis motor running test
Moving tests for all axes

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Select the items you wish to change.
Enter setting value and press Enter.
━ System Timing Setting
Year Setting
Month Setting
Day Setting
12 or 24 hour display format
Minute Setting
Hour Setting
Next Step
Next Step
Setup
You may set timing here.