Mecasys Optizen 1412V User manual

OPTIZEN 1412V User‟s Guide
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Optizen 1412V
Standard Version
For OPTIZEN 1412V
1st revision
July 2005
Mecasys Co., Ltd.
Notice:
Spectrophotometer Optizen® is a registered trademark
of Mecasys Co., Ltd. No part of the Optizen Series,
including this publication may be reproduced, or
modified in any form or by any means without the prior
permission of Mecasys Co., Ltd.
For more detail information on Optizen® or Optizen
View™, you may refer to “Technical Supports”in the
last Part of this guide or visit our websites below.
http://www.mecasys.co.kr/
Copyright © 1996 Mecasys Co.,Ltd. All rights reserved.

OPTIZEN 1412V User‟s Guide
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Part 1
Introduction & General Information
I. Introduction …4
1. SYSTEMS …5
Optical System
Sample Compartment
Modes
Electronics
Dimension
Print
Real-time PC Software (OptizenView)
2. Constructions …7
Exterior View
Front Side
Top Side
Rear Side
Left Side
Right Side
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Part 2
Optizen 1412V Usage
I. Control Panel …9
II. Main Function & Description …10
Switching On…
Main Screen Form (Measure Mode)
III. Usage of each Mode …11
1. STC Mode …11
Make STC
Modify STC
Delete STC
2. ABS/%T Mode …17
3. P/C Connect Mode …17
4. Open Data …17
Contents

OPTIZEN 1412V User‟s Guide
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IV. Measurement Procedure …18
1. STC Mode …18
2. ABS/%T Mode …21
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Part 3
Technical Supports
V. Technical Supports & A/S …23
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Appendix
Optizen 1412V String Table …24

OPTIZEN 1412V User‟s Guide
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I. Introduction
Thank you for purchasing our visible
Spectrophotometer Optizen 1412V.
Optizen 1412V, mounting monochromator
has high-precision and high-resolution,
and stabilizes voltage control and signal
processing with Noise Reduction Circuit.
Monochromator with a holographic grating
system, designed by our own technology
suggests better quality in precision and
accuracy of wavelength selection. This
holographic grating system is the up-to-
date technology used in a high-priced
precision instrument and ensures excellent reproducibility and
accuracy of measurement results.
By utilizing a holographic grating system, Optizen 1412V can
produce and select 760 single wavelengths from 340nm to 1100nm
at 1nm intervals.
Free voltage system allows connecting with any normal AC input
source. In addition electrical safety is certified with CE mark.
Part 1. Introduction & General Information

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1. SYSTEMS
OPTICAL SYSTEM
Spectral Bandwidth : < 3nm
Wavelength Range : 340 ~ 1100 nm
Accuracy : < 2nm
Repeatability : < 1nm
Monochromator : Czerny-turner type
Stray Light : <0.1 %T at 340nm
Photometric Range : -0.1 to 3.0A, 0 to 125%
Accuracy : 1% of reading at 1A
Stability : 0.003 A/h
Light Source : Halogen lamp
Wavelength Calibration : Self testing
SAMPLE COMPARTMENT
Standard rectangular cell holder (Optional)
16mm round cell holder
25mm round cell holder
MODES
STC Mode
ABS/%T Mode
P/C Connect Mode
View Mode
ELECTRONICS
External interface : RS-232(Optional)
Display : 128 * 64 Graphic LCD
Power Requirement : AC100 ~ 230V, 50/60H, 1A

OPTIZEN 1412V User‟s Guide
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DIMENSION
385(W)ⅹ344(D)ⅹ180(H),
Weight: 6.5kg
PRINT(Optional)
Printer : Thermal dot line print, 384 dots/line,
48mm print width with 8dots/mm resolution
Maximum Printing speeds: 200 dot lines/sec (25.0mm/sec)
REAL TIME PC SOFTWARE (Optional)
Interface Port: RS-232C port
PC OS : MS Windows-98/NT/2000
Capability :
ABS / %T / Conc.
Survey scan
Time base Scan
Absorbance ratio
User defined function
Inhibition Rate

OPTIZEN 1412V User‟s Guide
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2. Constructions
Exterior View
Front Side
Top Side
LCD Screen
Keypad
Print Outlet
Cell Holder Cover

OPTIZEN 1412V User‟s Guide
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Rear Side
Left Side
Right Side
Door for replacing ROM
and A/S
Power Cord
Receptacle
전원연결부
부
Power Switch
Cooling Fan
Rs-232c

OPTIZEN 1412V User‟s Guide
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I. Control Panel
1. [0, 1, …, 9] :input 0~9 Number
2. [Function]
[ESC] : move to upper menu
[PRINT] : cut printing paper
[HOME] : move to initial screen
[ABS %T] : mutually change measure-result to ABS or %T
[/ ]: move cursor to left (or back space key) / right
[(yes) / (no)] : scroll up / down or select yse / no
[ZERO] :measure Blank
[ENTER] : measure Sample or select menu
[ESC], [HOME], [PRINT] function is allowed at any face.
Part 2. Optizen 1412V Usage

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OPTIZEN 1412V
1. STC Mode
2. ABS/%T Mode
3. P/C Connect
4. Open Data
5. Print On(Off)
Select #(1 ~ 5)
OPTIZEN 1412V
Self Testing…
II. Main Function & Description
1. Switching On…
Logo screen is displayed and self-testing is
started for checking H/W condition.
Initializing spends 20~30 seconds; bad cell
requires more seconds than normal.
We recommend to warm up the equipment
during 15~30 minutes for the stability of
measurement.
2. Main Screen Form (Measure Mode)
An initial screen of Optizen 1412V is shown
as left picture. Select mode you want by
inputting the number of it.
Press [ENTER] after inputting the
number.
1. STC Mode : make a standard curve for measuring concentration with
this STC
2. ABS/%T Mode : measure Absorbance/Transmittance on specified
wavelength
3. P/C Connect : select when you use PC-interface S/W OptizenView
: Optional. Please contact your nearest dealer or distributor.
4. Open Data : see the saved data
5. Print On(Off) : set up print on/off

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STC Mode
Where to Save ?
STC Mode
1. Make STC
2. Modify STC
3. Delete STC
III. Usage of each MODE
1. STC Mode (Standard Curve Mode)
This is measuring mode by user-made and
saved standard curves. It is all EMPTY in
factory mode (initial state). You can save
maximum 100 standard curves.
You can activate Make, Modify and Delete
STC menu as left picture by inputting ‘999’
and pressing [ENTER] at previous screen,
Input #.
MAKE STC [1] Make STC
Input the location code to save the standard
curve and press [ENTER].
If you want to save # 1, input [1]..
STC Mode
1. EMPTY
2. EMPTY
3. EMPTY
4. EMPTY
5. EMPTY
6. EMPTY
INPUT #:

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STC Mode
Input STC Name.
––
STC Mode
1. mg/l 2. ug/l
3. ppm 4. ppt
5. % 6.NONE
Unit ?
STC Mode
Dilution ?
Input STC name with reference to string
table under Cell Compartment Cover.
You can be helped by Input method of the
Appendix.
Input the measure Wavelength and press
[ENTER].
Choose the value between 340 and 1100.
Select the unit of sample by input the
correct number.
Input the dilution factor. If the sample is too
thick to be out of measure limits, you have
to measure the sample after dilution. It will
be very convenient since you can see the
automatically calculated result with dilution
factor. In case of not diluted, input 1.
STC Mode
Wavelength(nm)
––––

OPTIZEN 1412V User‟s Guide
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STC Mode
Decimal ?
STC Mode
MAKE STC (Y/N) ?
STC Mode
Input Conc.1
STC Mode
Insert Blank
Press [ZERO]
You can choose the decimal places of the
result.
If you input [3], you can see the results
down to three decimal places.
(Maximum down to three decimal places)
Choose Make STC or use previous STC
factor that you already know.
[Y] : Make STC [N] : Input Factor
In case of choosing ‘Y’ at Make STC (Y/N)?
Insert Blank solution into the Cell Holder
and press [ZERO] key to measure the
Blank.
Input the lowest concentration of the
standard solutions and press [ENTER]

OPTIZEN 1412V User‟s Guide
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STC Mode
Conc.1
ABS= 0.235
Conc.2 (Y/N)?
STC Mode
Conc = 1.123 * x
+ 1.000
Using(Y/N)?
0,1,2,UP, ENTER
(X=ABS)
Conc.= aX^2
+ bX + c
R^2 = 1.00000
STC Mode
Conc.1 = 0.250
Insert Conc. 1,
Press [ENTER]
Check the inputted value and insert the
very standard solution into Cell holder. And
press [ENTER] to measure.
Check the measured absorbance value of
the first standard solution. If you want to
measure the second standard solution,
select [Y], else you want to make STC with
the one standard solution, press [N].
Repeat as many as the standards solutions.
We recommend using more than three
standards solutions to make a correct STC.
After measuring the all standard solutions,
select [N] at Conc.#n(Y/N)? and you can
see the formula of STC.
[0]: Linear cross zero
[1]: Linear
[2]: Quadratic
[UP] : See the Graph
[ENTER] : Confirm the formula
Select [Y] to apply the STC, or else select
[N] to make STC again at Using(Y/N)?.

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In case of choosing ‘N’at Make STC (Y/N)?
As you select to use previous STC factor
that you already know instead of making
the new STC, you can see the screen as
left.
Input the known STC factor to a, b and c
one by one. If STC is Linear form, input
zero to „a‟.
After input every factors, select whether
you will use the STC formula or not shown
as the picture left.
[Y] : Use the present STC formula.
[N] : Input STC factor again.
MODIFY STC
This mode is to check or modify parameter value of inputted STC
formula. Input the saved code number and press [ENTER]. And
then you can see the saved STC formula. You can modify the STC
formula the same way as Programming Mode.
STC Mode
y=aX^2 + bX + c
y=Conc. X=ABS
Input a:_
STC Mode
y=aX^2 + bX + c
y = Conc.X = ABS
Input b:_
STC Mode
y=aX^2 + bX + c
y = Conc.X = ABS
Input c:_
STC Mode
Conc = 0.01X^2
+ 0.150x
+ 0.001
Using (Y/N) ?

OPTIZEN 1412V User‟s Guide
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Delete STC
DELETE CODE #
__
Delete STC
Name:
AABBCC
Delete (Y/N)?
DELETE STC
This mode is to delete the existing STC formula. Input the saved
code number and press [ENTER]. And you can see the second
screen.

OPTIZEN 1412V User‟s Guide
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ABS / % T MODE
Input W.L.(nm)
____
P/C Connecting
PC Interface
Communication
Open Result List
1. ABCDE
2. EFGHI
3. <EMPTY>
4. <EMPTY>
5. <EMPTY>
6. <EMPTY>
7. <EMPTY>
Input File # :
2. ABS/%T Mode
Without concerning with specific parameter,
you can measure the simple absorbance
and transmittance on specified wavelength.
Input Wavelength and press [ENTER] to
measure.
3. P/C Connect
Select when you measure with P/C
interface S/W, OptizenView.
4. Open Data
You can see the saved data by inputting the
file number.

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STC Mode
1. ABCDE
2. EMPTY
3. EMPTY
4. EMPTY
5. EMPTY
6. EMPTY
INPUT # :
IV. Measurement Procedure
1. STC Mode
Select 1. STC Mode at initial Menu.
Select a measure item by input the number.
You can find the measure item with „↑‟, „↓‟
keys. Or you can select it by input strings
directly with reference to Appendix.
Measure Blank by pressing [ZERO] key
after inserting the Blank Sample.
STC Mode
Insert Blank
&
Press [ZERO]
OPTIZEN 1412V
1. STC Mode
2. ABS/%T Mode
3. P/C Connect
4. Open Data
Select #( 1 ~ 4)

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Insert the measure sample into Cell holder,
and press [ENTER] key to measure. If you
measure the same items successively, you
can keep measuring without Blank check by
press [ENTER] key.
Whenever you want to measure Blank again, press [ZERO] key
after insert Blank into Cell holder. And measure samples by
repeating as shown above.
Press ABS/%T Key to change the Concentration value to
Absorbance and transmittance value. If you want to measure
another sample, repeat as shown above after removing the former
sample and inserting new one.
On or after measuring, you can see the whole result on the screen
by pressing []key.
STC Mode
Insert Sample
Press [ENTER]
STC Mode
Name
Conc. =
30 mg/L
STC Mode
Name
0.262 ABS
54.65 %T

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Use Up, Down Key to scroll and see the
whole data.
[ENTER]: Save as files.
[ESC]: Move to previous menu
without saving.
[↑], [↓] : Check the whole data by
scrolling
You can save the result as files as below by Pressing [→] key.
If you input the file number, the result will
be saved in the file. You can open the data
by select 4. Open Data at initial Menu. You
can save maximum 20 Results in each file.
Input file name with reference to Strings
table in Appendix. If you don’t need to
input specific file name, press [ENTER]
to proceed.
STC Result
ABS CONC.
1)12.112 2.12
2)12.112 2.21
3)13.252 2.21
4)12.121 2.21
5)12.121 2.21
E N T E R to Save
Save STC Result
1.File #1
2.File #2
3.File #3
4.File #4
5.File #5
6.File #6
INPUT FILE #
File #2
Where to SAVE ?
3
Save ABS Result
INPUT FILE Name