Microfluidics MICROFLUIDIZER LV1 Series User manual

MODEL LV1 SERIES
MICROFLUIDIZER® PROCESSOR
User Manual
30 Ossipee Rd
P.O. Box 9101
Newton, MA 02464-9101
Phone [phone]
Fax [phone]
[e-mail]
www.microfluidicscorp.com
Part No. 85.0098

Copyright © 2011 Microfluidics International Corporation. All rights reserved.
This manual may not be copied, photocopied, reproduced, translated, converted to an electronic or machine-
readable form in whole or in part, or distributed to customers or other third parties without prior written
approval of Microfluidics International Corporation.

Model LV1 Microfluidizer Data Sheet
F-LV-1-5 8/3/2011 LV1 User Manual F-1
Model LV1 Microfluidizer Processor Data Sheet
Serial #:
Customer:
Intensifier
Ratio:
21:1 (Hydraulic Pressure: Process Pressure)
Process Piston Diameter:
0.437 in. (11.1 mm)
Stroke Length:
2.50 in. (63.5 mm)
Volume/Stroke:
0.375 in.3(6 ml)
Process Piston Seal:
UHMWPE (Ultra High Molecular Weight)
Gasket/O-rings:
Viton (standard)
Interaction Chamber
Style:
Serial #:
Model Specifications
Model Number
LV1
Approximate Process Discharge Flow
6 ml/stroke at full 2.5 inch per stroke
Maximum Product Processing Pressure
2069 bar (30,000 psi)
Product Inlet Pressure
Gravity feed
Inlet Reservoir Size
10 ml (0.61 cu-in)
Maximum Operating Hydraulic Pressure
124 bar (1,800 psi)
Maximum Hydraulic Oil Temperature
140°F (60°C)
Process Fluid Cooling Water
Required to cool from 161°F (72°C) to
70°F (21°C) [process temperature rises
approximately 3°F (1.7°C) per 1,000 psi
(69 bar) applied pressure]
Ice Water or Dry Ice used with optional cooling coil and tray
assembly
Intensifier Stroke Length
63.5 mm (2.50 inches)
Approximate Stroke Displacement
6 ml (.375 in3)
Approximate Strokes per Minute
Approximately 2 per minute (operator dependent) (see Chapter 3)

Model LV1 Microfluidizer Data Sheet
F-LV-1-5 8/3/2011 LV1 User Manual F-2
Model Number
LV1
Oil Reservoir Capacity (Approximate)
2.5 liters (0.7 gallon)
Motor Horse Power
0.37 kw (1/2 hp)
Motor Data
RPM
Voltage
Amperage
Phase
Frequency
1800 RPM
110V
10A
1 Phase
60 Hz
1800 RPM
208 -
230VAC
5A
1 Phase
60 Hz
1400 RPM
208 to
230VAC
5A
1 Phase
50 Hz
Approximate Unit Dimensions (WxDxH)
51.0 x 65.0 x 60.2 cm
(20.0” x 25.8” x 23.7”)
Approximate Gross Weight (with oil)
109 kg (240 lbs.)
Piston Seal Assembly
UHMWPE & PEEK
Coned and Threaded Fittings Torque
10 Nm (8.5 Ft-lb)
Nitrogen Volume/Charge Pressure for the
Accumulator
1 liter / 96.5 bar (1,400 psi)
Noise level less than 77 dBA.

Revision Page
2/18/2011 LV1 User Manual REV-1
Revision Page for LV1
This manual is Revision 10 for the LV1 Microfluidizer Processor and is dated September 7, 2011. It
consists of the following chapter revisions:
Chapter
Revision Code
Forward Data Sheet
F-LV-1-5 8/3/2011
Chapter 1: Introduction
1-LV-1-2 12/28/2010
Chapter 2: Overview
2-LV-1-5 8/3/11
Chapter 3: Operation
3-LV-1-7 9/7/11
Chapter 4: Maintenance
4-LV-1-4 12/28/2010
Chapter 5: Troubleshooting
5-LV-1-4 8/3/11
Appendix A: Drawing List
a-LV-1-6 8/3/11
Appendix B: Parts List
b-LV-1-5 8/3/11
Appendix C: Options
c-LV-1-3 12/28/2010
Appendix D: Fluid Compatibility Tables
fct-4 09/30/2010
Appendix E: Mobil Product Data Sheet
mpds-2 09/30/2010

Table of Contents
12/28/2010 LV1 User Manual TOC-1
Table of Contents
Table of Contents ...............................................................................................TOC-1
Model LV1 Microfluidizer Processor Data Sheet ..........................................................F-1
Intensifier.................................................................................................F-1
Interaction Chamber....................................................................................F-1
Model Specifications ....................................................................................F-1
Revision Page for LV1; Revision 3 ...........................................................................REV-1
Chapter 1: Introduction........................................................................................1-1
Inside this Manual .......................................................................................1-1
Disclaimers ...............................................................................................1-1
Design Change ...........................................................................................1-1
Reproduction.............................................................................................1-1
Shipping...................................................................................................1-1
Customer Support.......................................................................................1-1
Warranty..................................................................................................1-2
Chapter 2: Overview ...........................................................................................2-1
General Description.....................................................................................2-1
Major Components ......................................................................................2-1
Principle of Operation..................................................................................2-1
Hydraulic Power System................................................................................2-2
High Pressure Intensifier ...............................................................................2-2
Interaction Chamber....................................................................................2-2
Controls and Connections..............................................................................2-2
Chapter 3: Operation...........................................................................................3-1
Safety Warnings & Cautions ...........................................................................3-1
System Description......................................................................................3-2
Supplied Items ...........................................................................................3-2
Items Not Supplied......................................................................................3-3
Site Preparation .........................................................................................3-3
Foundation/Benchtop.............................................................................3-4
Clearances..........................................................................................3-4
Moving the Equipment............................................................................3-4
Dimensions ...............................................................................................3-4
Utility Requirements....................................................................................3-4
Electrical (General) ...............................................................................3-4
Information on Charging Accumulators ........................................................3-4
Environmental Requirements....................................................................3-7
Preparing Utility Hookups..............................................................................3-7
General....................................................................................................3-7
Preparing for Use........................................................................................3-8
Purging Air from the Hydraulic System and Priming the Intensifier Pump ............... 3-8
Hydraulic Preparation to be Done Upon Initial Setup or Use After
Long Storage Periods..............................................................................3-8
Standard Preparations for Use...................................................................3-8

Table of Contents
12/28/2010 LV1 User Manual TOC-2
Operating the Equipment ..............................................................................3-9
Filling the Product Inlet Reservoir..............................................................3-9
Duty Cycle................................................................................................3-11
Cleaning and Storing the Equipment.................................................................3-11
Using the Emergency Stop .............................................................................3-11
Chapter 4: Maintenance .......................................................................................4-1
Replacing Piston Seal Assembly.......................................................................4-1
Removing the Liquid End Body ..................................................................4-1
Removing the Piston, Bearing Retainer and Bearing.........................................4-4
Cleaning Components.............................................................................4-6
Installing the Piston, Seal, Bearing Retainer, Bearings and Backup Rings................4-6
Installing the Isolator (If Removed).............................................................4-8
Installing the Liquid End Body...................................................................4-9
Check Valve Maintenance..............................................................................4-10
Cleaning/Repairing the Inlet Check Valve....................................................4-10
Outlet Valve Maintenance..............................................................................4-11
Replacing the Hydraulic Oil............................................................................4-12
Cleaning the Hydraulic Oil Reservoir & Servicing the Suction Strainer and
Return Line Filter .......................................................................................4-12
Return Filter Service Schedule .................................................................4-13
Hydraulic Cylinder.......................................................................................4-13
Hydraulic Cylinder Disassembly ................................................................4-13
Reservoir Filler Breather Cap Replacement.........................................................4-14
Coned and Threaded Connections Maintenance....................................................4-14
Chapter 5: Troubleshooting...................................................................................5-1
Monitoring the Pressure ................................................................................5-1
Unplugging the Chamber...............................................................................5-1
Maintaining Connections and Fittings ................................................................5-2
Motor Problems Table ..................................................................................5-6
Appendix A: Drawing List......................................................................................A-1
List of Drawings for LV1................................................................................A-1
Appendix B: Tool & Spare Parts List ........................................................................B-1
Standard Tool Kit........................................................................................B-1
Standard Spare Parts Kit ...............................................................................B-1
Appendix C: Options............................................................................................C-1
Model LV1 Options.......................................................................................C-1
Appendix D: Fluid Compatibility Tables....................................................................D-1
The Elastomers ..........................................................................................D-1
The Plastics...............................................................................................D-1
Appendix E: Mobil Product Data Sheet .....................................................................E-1
Mobil DTE® 20 Series Super-Stabilized, Antiwear Hydraulic Oils.................................E-1
Description .........................................................................................E-1
Typical Characteristics ...........................................................................E-1
Applications ........................................................................................E-1
Advantages .........................................................................................E-1
Health and Safety .................................................................................E-2

Table of Contents
12/28/2010 LV1 User Manual TOC-3
Mobil DTE 25 .............................................................................................E-2

Chapter 1: Introduction
1-LV-1-2 12/28/2010 LV1 User Manual 1-1
Chapter 1: Introduction
Inside this Manual
This manual is intended as a guide for the operator of the LV1 (Low Volume) Series Microfluidizer®
materials processor. It contains 5 chapters and 5 appendices. Chapter 1 gives an introduction to
this manual and how to find additional support. Chapter 2 offers a general overview to the
Microfluidizer with a description of the specific parts of the machine you will want to identify for
operation. Chapter 3 explains proper installation and operation procedures. Chapter 4 contains a
general description of the maintenance of the LV1 Series Microfluidizer equipment. Chapter 5
contains troubleshooting information which will help you obtain the best possible results with your
processor. The appendices include a drawings list, tool & spare parts list, options list, fluid
compatibility tables, and a Mobil product data sheet.
Disclaimers
Due to design changes and product improvements, information in this manual is subject to change
without notice. Microfluidics (hereafter called “MFIC”) reserves the right to change design at any
time, which may subsequently affect the contents of this manual.
Design Change
MFIC will make every reasonable effort to ensure that this user manual is up to date and
corresponds with your shipped LV1 Series Microfluidizer equipment.
Reproduction
Neither this manual nor any part of it may be reproduced, photocopied, or electronically
transmitted in any way without the advanced written permission of MFIC.
Shipping
MFIC is not responsible for damage to equipment incurred during shipping and only furnishes
replacement parts against a written purchase order. It is the customer’s responsibility to file a
claim with the carrier.
Customer Support
The information given here is general. Some applications may require additional procedure or
equipment modification. For further assistance, feel free to contact the Customer Service
Department at Microfluidics International Corporation at 1-800-370-5452. Hours are 8:30 am - 5:00
pm EST, Monday through Friday.

Chapter 1: Introduction
1-LV-1-2 12/28/2010 LV1 User Manual 1-2
Warranty
The sole warranty provided by the Company is contained in the "Microfluidics Corporation
Standard Terms and Conditions", Form M-5 provided at time of purchase.