Microfluidics MICROFLUIDIZER LV1 Series User manual

MODEL LV1 SERIES
MICROFLUIDIZER® PROCESSOR
User Manual
30 Ossipee Rd
P.O. Box 9101
Newton, MA 02464-9101
Phone [phone]
Fax [phone]
[e-mail]
www.microfluidicscorp.com
Part No. 85.0098

Copyright © 2011 Microfluidics International Corporation. All rights reserved.
This manual may not be copied, photocopied, reproduced, translated, converted to an electronic or machine-
readable form in whole or in part, or distributed to customers or other third parties without prior written
approval of Microfluidics International Corporation.

Model LV1 Microfluidizer Data Sheet
F-LV-1-5 8/3/2011 LV1 User Manual F-1
Model LV1 Microfluidizer Processor Data Sheet
Serial #:
Customer:
Intensifier
Ratio:
21:1 (Hydraulic Pressure: Process Pressure)
Process Piston Diameter:
0.437 in. (11.1 mm)
Stroke Length:
2.50 in. (63.5 mm)
Volume/Stroke:
0.375 in.3(6 ml)
Process Piston Seal:
UHMWPE (Ultra High Molecular Weight)
Gasket/O-rings:
Viton (standard)
Interaction Chamber
Style:
Serial #:
Model Specifications
Model Number
LV1
Approximate Process Discharge Flow
6 ml/stroke at full 2.5 inch per stroke
Maximum Product Processing Pressure
2069 bar (30,000 psi)
Product Inlet Pressure
Gravity feed
Inlet Reservoir Size
10 ml (0.61 cu-in)
Maximum Operating Hydraulic Pressure
124 bar (1,800 psi)
Maximum Hydraulic Oil Temperature
140°F (60°C)
Process Fluid Cooling Water
Required to cool from 161°F (72°C) to
70°F (21°C) [process temperature rises
approximately 3°F (1.7°C) per 1,000 psi
(69 bar) applied pressure]
Ice Water or Dry Ice used with optional cooling coil and tray
assembly
Intensifier Stroke Length
63.5 mm (2.50 inches)
Approximate Stroke Displacement
6 ml (.375 in3)
Approximate Strokes per Minute
Approximately 2 per minute (operator dependent) (see Chapter 3)

Model LV1 Microfluidizer Data Sheet
F-LV-1-5 8/3/2011 LV1 User Manual F-2
Model Number
LV1
Oil Reservoir Capacity (Approximate)
2.5 liters (0.7 gallon)
Motor Horse Power
0.37 kw (1/2 hp)
Motor Data
RPM
Voltage
Amperage
Phase
Frequency
1800 RPM
110V
10A
1 Phase
60 Hz
1800 RPM
208 -
230VAC
5A
1 Phase
60 Hz
1400 RPM
208 to
230VAC
5A
1 Phase
50 Hz
Approximate Unit Dimensions (WxDxH)
51.0 x 65.0 x 60.2 cm
(20.0” x 25.8” x 23.7”)
Approximate Gross Weight (with oil)
109 kg (240 lbs.)
Piston Seal Assembly
UHMWPE & PEEK
Coned and Threaded Fittings Torque
10 Nm (8.5 Ft-lb)
Nitrogen Volume/Charge Pressure for the
Accumulator
1 liter / 96.5 bar (1,400 psi)
Noise level less than 77 dBA.

Revision Page
2/18/2011 LV1 User Manual REV-1
Revision Page for LV1
This manual is Revision 10 for the LV1 Microfluidizer Processor and is dated September 7, 2011. It
consists of the following chapter revisions:
Chapter
Revision Code
Forward Data Sheet
F-LV-1-5 8/3/2011
Chapter 1: Introduction
1-LV-1-2 12/28/2010
Chapter 2: Overview
2-LV-1-5 8/3/11
Chapter 3: Operation
3-LV-1-7 9/7/11
Chapter 4: Maintenance
4-LV-1-4 12/28/2010
Chapter 5: Troubleshooting
5-LV-1-4 8/3/11
Appendix A: Drawing List
a-LV-1-6 8/3/11
Appendix B: Parts List
b-LV-1-5 8/3/11
Appendix C: Options
c-LV-1-3 12/28/2010
Appendix D: Fluid Compatibility Tables
fct-4 09/30/2010
Appendix E: Mobil Product Data Sheet
mpds-2 09/30/2010

Table of Contents
12/28/2010 LV1 User Manual TOC-1
Table of Contents
Table of Contents ...............................................................................................TOC-1
Model LV1 Microfluidizer Processor Data Sheet ..........................................................F-1
Intensifier.................................................................................................F-1
Interaction Chamber....................................................................................F-1
Model Specifications ....................................................................................F-1
Revision Page for LV1; Revision 3 ...........................................................................REV-1
Chapter 1: Introduction........................................................................................1-1
Inside this Manual .......................................................................................1-1
Disclaimers ...............................................................................................1-1
Design Change ...........................................................................................1-1
Reproduction.............................................................................................1-1
Shipping...................................................................................................1-1
Customer Support.......................................................................................1-1
Warranty..................................................................................................1-2
Chapter 2: Overview ...........................................................................................2-1
General Description.....................................................................................2-1
Major Components ......................................................................................2-1
Principle of Operation..................................................................................2-1
Hydraulic Power System................................................................................2-2
High Pressure Intensifier ...............................................................................2-2
Interaction Chamber....................................................................................2-2
Controls and Connections..............................................................................2-2
Chapter 3: Operation...........................................................................................3-1
Safety Warnings & Cautions ...........................................................................3-1
System Description......................................................................................3-2
Supplied Items ...........................................................................................3-2
Items Not Supplied......................................................................................3-3
Site Preparation .........................................................................................3-3
Foundation/Benchtop.............................................................................3-4
Clearances..........................................................................................3-4
Moving the Equipment............................................................................3-4
Dimensions ...............................................................................................3-4
Utility Requirements....................................................................................3-4
Electrical (General) ...............................................................................3-4
Information on Charging Accumulators ........................................................3-4
Environmental Requirements....................................................................3-7
Preparing Utility Hookups..............................................................................3-7
General....................................................................................................3-7
Preparing for Use........................................................................................3-8
Purging Air from the Hydraulic System and Priming the Intensifier Pump ............... 3-8
Hydraulic Preparation to be Done Upon Initial Setup or Use After
Long Storage Periods..............................................................................3-8
Standard Preparations for Use...................................................................3-8

Table of Contents
12/28/2010 LV1 User Manual TOC-2
Operating the Equipment ..............................................................................3-9
Filling the Product Inlet Reservoir..............................................................3-9
Duty Cycle................................................................................................3-11
Cleaning and Storing the Equipment.................................................................3-11
Using the Emergency Stop .............................................................................3-11
Chapter 4: Maintenance .......................................................................................4-1
Replacing Piston Seal Assembly.......................................................................4-1
Removing the Liquid End Body ..................................................................4-1
Removing the Piston, Bearing Retainer and Bearing.........................................4-4
Cleaning Components.............................................................................4-6
Installing the Piston, Seal, Bearing Retainer, Bearings and Backup Rings................4-6
Installing the Isolator (If Removed).............................................................4-8
Installing the Liquid End Body...................................................................4-9
Check Valve Maintenance..............................................................................4-10
Cleaning/Repairing the Inlet Check Valve....................................................4-10
Outlet Valve Maintenance..............................................................................4-11
Replacing the Hydraulic Oil............................................................................4-12
Cleaning the Hydraulic Oil Reservoir & Servicing the Suction Strainer and
Return Line Filter .......................................................................................4-12
Return Filter Service Schedule .................................................................4-13
Hydraulic Cylinder.......................................................................................4-13
Hydraulic Cylinder Disassembly ................................................................4-13
Reservoir Filler Breather Cap Replacement.........................................................4-14
Coned and Threaded Connections Maintenance....................................................4-14
Chapter 5: Troubleshooting...................................................................................5-1
Monitoring the Pressure ................................................................................5-1
Unplugging the Chamber...............................................................................5-1
Maintaining Connections and Fittings ................................................................5-2
Motor Problems Table ..................................................................................5-6
Appendix A: Drawing List......................................................................................A-1
List of Drawings for LV1................................................................................A-1
Appendix B: Tool & Spare Parts List ........................................................................B-1
Standard Tool Kit........................................................................................B-1
Standard Spare Parts Kit ...............................................................................B-1
Appendix C: Options............................................................................................C-1
Model LV1 Options.......................................................................................C-1
Appendix D: Fluid Compatibility Tables....................................................................D-1
The Elastomers ..........................................................................................D-1
The Plastics...............................................................................................D-1
Appendix E: Mobil Product Data Sheet .....................................................................E-1
Mobil DTE® 20 Series Super-Stabilized, Antiwear Hydraulic Oils.................................E-1
Description .........................................................................................E-1
Typical Characteristics ...........................................................................E-1
Applications ........................................................................................E-1
Advantages .........................................................................................E-1
Health and Safety .................................................................................E-2

Table of Contents
12/28/2010 LV1 User Manual TOC-3
Mobil DTE 25 .............................................................................................E-2

Chapter 1: Introduction
1-LV-1-2 12/28/2010 LV1 User Manual 1-1
Chapter 1: Introduction
Inside this Manual
This manual is intended as a guide for the operator of the LV1 (Low Volume) Series Microfluidizer®
materials processor. It contains 5 chapters and 5 appendices. Chapter 1 gives an introduction to
this manual and how to find additional support. Chapter 2 offers a general overview to the
Microfluidizer with a description of the specific parts of the machine you will want to identify for
operation. Chapter 3 explains proper installation and operation procedures. Chapter 4 contains a
general description of the maintenance of the LV1 Series Microfluidizer equipment. Chapter 5
contains troubleshooting information which will help you obtain the best possible results with your
processor. The appendices include a drawings list, tool & spare parts list, options list, fluid
compatibility tables, and a Mobil product data sheet.
Disclaimers
Due to design changes and product improvements, information in this manual is subject to change
without notice. Microfluidics (hereafter called “MFIC”) reserves the right to change design at any
time, which may subsequently affect the contents of this manual.
Design Change
MFIC will make every reasonable effort to ensure that this user manual is up to date and
corresponds with your shipped LV1 Series Microfluidizer equipment.
Reproduction
Neither this manual nor any part of it may be reproduced, photocopied, or electronically
transmitted in any way without the advanced written permission of MFIC.
Shipping
MFIC is not responsible for damage to equipment incurred during shipping and only furnishes
replacement parts against a written purchase order. It is the customer’s responsibility to file a
claim with the carrier.
Customer Support
The information given here is general. Some applications may require additional procedure or
equipment modification. For further assistance, feel free to contact the Customer Service
Department at Microfluidics International Corporation at 1-800-370-5452. Hours are 8:30 am - 5:00
pm EST, Monday through Friday.

Chapter 1: Introduction
1-LV-1-2 12/28/2010 LV1 User Manual 1-2
Warranty
The sole warranty provided by the Company is contained in the "Microfluidics Corporation
Standard Terms and Conditions", Form M-5 provided at time of purchase.

Chapter 2: Overview
2-LV-1-5 8/3/11 LV1 User Manual 2-1
Chapter 2: Overview
Chapter 2 includes detailed information about the principle of operation, hydraulic power system,
high pressure intensifier, interaction chamber, controls, and system specifications.
General Description
The LV1 Series Microfluidizer Processor is designed for high efficiency cell rupturing and high-
performance micro-mixing of emulsions and dispersions in a variety of industries. The LV1 model is
specifically designed to process small volumes and small batches and as such was engineered with
limited duty cycle (described in Chapter 3). Designed to suit industrial laboratory environments,
the LV1 Microfluidizer Processor requires relatively low volume of material to process.
Major Components
The LV1 Microfluidizer Processor is made up of the following major components:
•Hydraulic Power System, which provides power to the Microfluidizer equipment
•High Pressure Single-Acting Intensifier Pump, which multiplies the pressure supplied from
the hydraulic power system to an optimal operating level
•Interaction Chamber (IXC), where size reduction of the product occurs; the product is
accelerated to a high velocity and is subjected to intense shear and impact. The standard
LV1 unit comes with a ceramic interaction chamber (CIXC).
•An optional cooling coil and tray which cools the product stream as it exits the system
Principle of Operation
Power for the LV1 Microfluidizer Processor is generated by an on-board hydraulic power system,
which supplies pressurized hydraulic oil to a single-acting intensifier pump. The intensifier pump
amplifies the hydraulic pressure and transfers it to liquid pressure in the process stream. Working
process pressure levels as high as 2069 bar (30,000 psi) can be reached.
During the suction stroke, the intensifier pump draws product from the inlet reservoir/syringe into
the liquid end via the inlet check valve, which closes during the power stroke. During the power
stroke, the product stream is elevated to a constant pressure level.
The pressurized product stream enters the interaction chamber and passes through geometrically
fixed micro-channels, causing it to accelerate to very high velocities. It is here that the product
stream is acted upon by two primary forces, which bring about the desired results:
•Shear - deformation of the product stream; occurring from contact with channel walls at
high velocity.
•Impact - collision; occurring when the high velocity product stream impinges upon itself.
Upon exiting the interaction chamber (IXC), a heat exchanger (optional cooling coil and tray) may
return the product stream to ambient temperature.

Chapter 2: Overview
2-LV-1-5 8/3/11 LV1 User Manual 2-2
Hydraulic Power System
The hydraulic power system provides power to the Microfluidizer equipment via pressurized
hydraulic fluid. Standard hydraulic components make up the hydraulic power system. See the
drawing on the CD.
High Pressure Intensifier
The high pressure intensifier multiplies the pressure supplied from the hydraulic power system via
the hydraulic fluid to the necessary operating level. The intensifier is located in the front of the
Microfluidizer equipment, and is accessible for servicing, including removal for autoclaving.
Designed to operate with simple linear motion, the intensifier requires minimal maintenance. See
the drawing "Liquid End Pump Assembly.”
Interaction Chamber
See the Principle of Operation section. The interaction chamber can only be mounted in one way.
It is easily accessible for cleaning or replacing.
Constructed of stainless steel with ceramic product contact surfaces, the interaction chamber is
dimensionally fixed. Microfluidics supplies the interaction chamber in the correct size according to
each customer’s product requirements and specifications.
Controls and Connections
This section describes the various controls, valves, gauges, connections, and components on the
LV1. Figure 2.1 shows a front view of the LV1. Figure 2.2 shows a rear view on the LV1.
Descriptions of components are supplied in the following subsections.

Chapter 2: Overview
2-LV-1-5 8/3/11 LV1 User Manual 2-3
Figure 2.1. Front Panel Controls/Components
Control/Component Name
Description
A. Power-On Light
Green. Comes on when power is delivered to the unit.
B. Ready Light
Green. Indicates it is OK to extend piston at high pressure.
C. Piston Retract
Retracts piston in Jog mode only.
D. Piston Extend
Extends piston in Jog (low pressure) and Run (high pressure) mode.
E. Pump On Light
A white light that Indicates that the hydraulic pump is operating.
F. Mode Switch
Controls jogging of process piston and high-pressure piston extension
in the Run position.
G. Emergency Stop
Shuts off power to the intensifier and motor.
H. Outlet Reservoir
Includes 10 ml syringe.
I. Interaction Chamber
Refer to the Interaction Chamber section.
J. Inlet Reservoir
Includes 10 ml syringe.
K. Intensifier Pump
Houses high pressure inducing elements
L. Process Pressure Adjustment
Rotating the knob counterclockwise decreases process pressure.
Rotating the knob clockwise increases process pressure.

Chapter 2: Overview
2-LV-1-5 8/3/11 LV1 User Manual 2-4
Control/Component Name
Description
M. Process Pressure Gauge
Has a range from 0 to 40,000 psi. Note that the pressure being
monitored is NOT product pressure, but scaled hydraulic pressure that
represents the product process pressure.
N. Reset
Allows delivery of power upon system initialization.
O. Oil Fill
P. Accumulator Charge Port
Q. Directional Control Valve
R. Master Power On/Owith Lockout Device
S. Electrical Box
Figure 2.2. Rear View
Control/Component Name
Description
O. Oil Fill
Provides access for filling with hydraulic oil. Note: the oil must be
pre-filtered to at least 10 microns. Refer to Appendix E for the
recommended grade.
P. Accumulator Charge Port
Provides access for charging the accumulator.
Q. Directional Control Valve
Controls the flow direction of the hydraulic fluid.
R. Master Power On/Off
Allows power to enter the unit.
S. Electrical Box

Chapter 3: Operation
3-LV-1-7 9/7/11 LV1 User Manual 3-1
Chapter 3: Operation
Safety Warnings & Cautions
"WARNING” indicates that if a specific procedure or practice is not followed correctly, bodily
injury could occur.
"CAUTION" indicates that if a specific procedure or practice is not observed, damage to the
equipment could result.
WARNING: ENSURE THAT ALL MICROFLUIDIZER PROCESSOR SAFETY DEVICES ARE WORKING
PROPERLY AND THAT THE PROTECTIVE COVER AND PANELS ARE IN PLACE.
WARNING: NEVER TIGHTEN FITTINGS OR ADJUST THE MICROFLUIDIZER EQUIPMENT WHILE IT IS
UNDER PRESSURE OR WHEN IT IS RUNNING.
WARNING: DO NOT USE DAMAGED MICROFLUIDIZER EQUIPMENT OR EQUIPMENT THOUGHT TO
BE DAMAGED.
WARNING: KEEP ALL BODY PARTS AND LOOSE CLOTHING AWAY FROM THE MOVING PARTS OF
THE MICROFLUIDIZER EQUIPMENT.
WARNING: THIS EQUIPMENT CONTAINS A NITROGEN CHARGED ACCUMULATOR. IT MUST BE
DISCHARGED BEFORE SHIPPING AND BEFORE WORKING ON ANY HYDRAULIC COMPONENTS.
WARNING: DO NOT ATTEMPT TO LIFT THE MICROFLUIDIZER EQUIPMENT BY ANY OF THE
MOUNTED COMPONENTS. SERIOUS PERSONAL INJURY AND/OR DAMANGE TO THE EQUIPMENT
COULD RESULT. THE MICROFLUIDIZER EQUIPMENT WEIGHS APPROXIMATELY 240 LB (109 KG).
CAUTION: IF THE MICROFLUIDIZER PROCESSOR IS NOT GOING TO BE USED FOR MORE THAN ONE
MONTH, THE ACCUMULATOR NITROGEN GAS MUST BE DISCHARGED AND RECHARGED PRIOR TO
THE NEXT USE.
WARNING: THE MICROFLUIDIZER EQUIPMENT IS SHIPPED WITHOUT HYDRAULIC OIL IN THE
RESERVOIR. USERS MUST FILL THE RESERVOIR WITH HYDRAULIC FLUID THAT HAS BEEN
FILTERED THROUGH A 10 MICRON FILTER BEFORE USING THE EQUIPMENT (OIL IS INCLUDED IN
TOOL KIT). ALSO, IF SIGNIFICANT MOVING IS REQUIRED, REMOVE THE HYDRAULIC OIL. DO NOT
START THE MOTOR WITHOUT ENSURING THAT A SUFFICIENT LEVEL OF HYDRAULIC OIL IS IN

Chapter 3: Operation
3-LV-1-7 9/7/11 LV1 User Manual 3-2
THE TANK. DAMAGE TO THE PUMP COULD RESULT. DURING OIL FILL, EYE PROTECTION MUST BE
WORN.
WARNING: THE ACCUMULATOR MUST BE CHARGED BEFORE THE MACHINE CAN BE OPERATED.
SEE SECTION ON ACCUMULATOR CHARGING. DURING CHARGING, EYE PROTECTION MUST BE
WORN.
WARNING: PLACING A RESTRICTIVE DEVICE ON THE PROCESSED PRODUCT OUTLET MAY DAMAGE
THE DOWNSTREAM TUBING AND FITTINGS.
WARNING: WHEN RUNNING THE MACHINE WITH THE COVER OFF (PURGING OIL BUBBLES AFTER
FILLING HYDRAULIC OIL), EXTREME CAUTION MUST BE TAKEN DUE TO ELECTRICAL SHOCK AND
EYE PROTECTION MUST BE WORN.
WARNING: IF PROCESSING CHEMICALS THAT PRODUCE HARMFUL VAPORS, MACHINE MUST BE
RUN UNDER A LABORATORY HOOD.
System Description
The LV1 includes the following:
•Hydraulic Power System
•High-Pressure Intensifier Pump, single acting
•Interaction Chamber
•Motor Starter with overload protection
•1/2 hp (0.37 kw) 1 phase electric motor
•Gauge for displaying process pressure
•Product outlet tube
•10 ml syringe inlet reservoir
•10 ml syringe outlet reservoir
Supplied Items
The following items are supplied with the LV1:
•Oil transfer / suction pump
•1 gallon hydraulic oil
•Tool Kit (refer to Appendix B)
•Spare Parts Kit (refer to Appendix B)
•Lockout device on Master Power switch

Chapter 3: Operation
3-LV-1-7 9/7/11 LV1 User Manual 3-3
Items Not Supplied
The following items are NOT supplied with the unit:
•Compressed nitrogen @ 96.5 bar (1,400 psi)
•External safety device on the feed supply or product discharge lines
Site Preparation
NOTE:Check the shipping container and its contents for damage. If damage is suspected, notify
both the carrier and MFIC immediately on the straight bill of lading (supplied by the
carrier). If any damage is found, you may request an inspection by the carrier. Such
requests should be made as quickly as possible.
•Compare the contents of the packing list against the contents of the shipping container to
ensure receipt of all components.
•Locate the desired operating site and prepare sufficient space for the equipment (refer to
Figure 3.1).
•Ensure that the proposed operating site meets all the environmental specifications listed
in this chapter (refer to the Environmental Requirements section).
•Make available sufficient electric power. Then, connect to the system (refer to the Utility
Requirements section).
NOTE:We recommend saving the shipping container for possible future use (i.e., in case you need
to return the equipment for service).
Figure 3.1 Site Plan Clearance

Chapter 3: Operation
3-LV-1-7 9/7/11 LV1 User Manual 3-4
Foundation/Benchtop
The foundation or benchtop of the proposed operating site MUST be able to support the gross
weight of the Microfluidizer equipment, including oil.
Clearances
A minimum clearance of 10 inches (25.4 cm) around all sides of the Microfluidizer equipment is
required.
Moving the Equipment
A forklift or a hand truck should be used when lifting the Microfluidizer equipment from the
shipping crate to its new location. Make certain the lifting device is rated for the maximum weight
of the equipment. See below for forklift location.
Lift with forklift here
Dimensions
Width
Height
Depth
Weight (with oil)
51.0 cm
(20 in)
60.2 cm
(23.7 in.)
(65 cm)
(25.8 in)
(109 kg)
(240 lb.)
Utility Requirements
Electrical (General)
Electrical requirements vary by location.
Voltage
Current
Phase
Frequency
110/208 to 230VAC
10/5
1
60 Hz
208 to 230VAC
5 Amps
1
50 Hz

Chapter 3: Operation
3-LV-1-7 9/7/11 LV1 User Manual 3-5
The LV1 is supplied with a motor starter that includes On/Off switching.
The LV1 must be properly grounded according to national and local electrical codes. The end user
must supply an electrical circuit breaker and disconnect for the LV1.All fuses and relays are
located within the electrical box of the unit.
Information on Charging Accumulators
WARNING: THE USE OF OXYGEN OR AIR WILL RESULT IN AN EXPLOSION OF THE ACCUMULATOR.
USE OF OXYGEN IS STRICTLY PROHIBITED. CHARGE THE ACCUMULATOR WITH DRY NITROGEN
OR AUTHORIZED INERT GASES ONLY.
Nitrogen Charge Pressure
The nitrogen charge pressure for the accumulator should be between 1400 and 1450 psi maximum.
This pressure range should not be exceeded.
CAUTION: NEVER TURN ON THE PUMP WITHOUT FIRST CHARGING THE ACCUMULATOR.
Charging an Accumulator
If this is an initial setup, please refer to the instructions in the Preparing for Use section of this
chapter. The part number of the US charging kit is 80.0006. For other countries the appropriate
charging kits must be selected.
1. Make sure the nitrogen supply is shut off.
2. Attach the hose to the nitrogen bottle.
3. Remove the gas valve guard and gas valve cap from the accumulator.
4. Back the gas chuck “T” handle all the way out (counterclockwise) before attaching the
charging assembly to the accumulator gas valve.
5. Close the bleed valve.
6. Making sure not to loop or twist the hose, attach the swivel nut to the gas valve and
tighten (10-15 in. lb.) (11.5-17 cm kg).
7. Turn the gas chuck “T” handle clockwise until the gauge starts to show pressure in the
accumulator.
CAUTION: DO NOT TURN THE “T” HANDLE ALL THE WAY DOWN, AS IT WILL DAMAGE THE
VALVE CORE.
8. Slowly crack open the nitrogen bottle valve and slowly fill the accumulator.
Shut it off when the gauge indicates the desired pre-charge (96.5 bar; 1,400 psi).

Chapter 3: Operation
3-LV-1-7 9/7/11 LV1 User Manual 3-6
9. Let the pre-charge set for 10-15 minutes. This will allow the gas temperature to
stabilize.
If the desired pre-charge is exceeded, close the nitrogen bottle valve, and then slowly
open the bleed valve (refer to Figure 3.2).
CAUTION: DO NOT REDUCE THE PRE-CHARGE BY DEPRESSING THE VALVE CORE WITH A
FOREIGN OBJECT. HIGH PRESSURE MAY RUPTURE THE RUBBER VALVE SEAT.
10. When finished pre-charging the accumulator, turn the “T” handle counterclockwise all
the way out on the gas chuck (refer to Figure 3.2), then open the bleed valve.
11. Hold the gas valve to keep from turning, loosen the swivel nut, and remove the
assembly. Check for pre-charge leaks using a common leak reactant.
12. Replace the gas valve cap (10-15 in. lbs.) (11.5-17 cm kg) and valve guard. (Gas valve
cap serves as a secondary seal.)
Figure 3.2 Charging Components
WARNING: THIS EQUIPMENT CONTAINS A NITROGEN CHARGED ACCUMULATOR. IT MUST BE
DISCHARGED BEFORE SHIPPING.
Hydraulic Oil
CAUTION: CUSTOMERS SHOULD NOT START THE MOTOR WITHOUT ENSURING THAT A
SUFFICIENT LEVEL OF OIL IS IN THE TANK. DAMAGE TO THE PUMP AND HYDRAULIC SYSTEM
COULD RESULT.
NOTE:Use Mobil DTE 25 oil (or equivalent) filtered through a 10 micron filter.
Filling the Hydraulic Oil Reservoir
1. Remove the fill/vent cap from the hydraulic oil reservoir fill tube.