Milestone DMA-80 evo User manual

DMA-80
Operator Manual
MA213
MILESTONE
HELPING
CHEMISTS

Milestone Srl MA213-003 – DMA-80 EVO – User Manual
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Thank you for selecting our DMA-80 system.
We are sure that you will be completely satisfied with the performance of this
new unit entering your laboratory. We invite you to read carefully this user
manual and to keep it in reach for convenient and fast consulting. The person
who will be using this unit should have received an appropriate training from
a Milestone trained technician. For any possible clarification or any request
for assistance please contact either our Representative in your country:
or Milestone at the following address:
Milestone Srl
Via Fatebenefratelli, 1/5
24010 Sorisole (BG) Italy
Tel [phone]
Fax [phone]
Website www.milestonesrl.com
E-mail [e-mail]
Publication date (gg/mm/aaaa) 24/09/2021

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1 SAFETY RULES ...................................................... 6
1.1 General Information .............................................................. 6
1.2 Safety rules for start-up ........................................................ 6
1.3 Safety rules in operations ..................................................... 6
1.4 Safety in service operations .................................................. 7
1.5 No manipulation on the unit allowed ..................................... 7
1.6 Parts replacement ................................................................ 7
1.7 Customer support ................................................................. 7
1.8 Other hints ............................................................................ 7
1.8.1 Safety of operation ............................................................................... 7
1.9 Cleaning instructions ............................................................ 8
1.10 Maintenance instructions ...................................................... 8
1.10.1 Fuses ................................................................................................. 8
2 INSTALLATION ....................................................... 9
2.1 Unpacking and checking list ................................................. 9
2.1.1 DMA-80 EVO STANDARD EQUIPMENT........................................... 10
2.2 CATALYST AND AMALGAMATOR INSTALLATION .......... 13
2.3 DMA-80 EVO SETUP ......................................................... 20
2.3.1 Voltage setting ................................................................................... 21
2.3.2 DMA connection with Terminal 660.................................................... 24
2.3.3 DMA 80 EVO connection with PC ...................................................... 25
2.3.4 DMA safety switch ............................................................................. 29
2.4 Gas connection ................................................................... 29
2.4.1 Oxygen tank ...................................................................................... 30
2.4.2 Milestone Air compressor .................................................................. 30
3 DMA-80 EVO GENERAL INFORMATION............. 34
3.1 Technical data .................................................................... 34
3.2 DMA-80 EVO front view ...................................................... 36
3.3 DMA-80 EVO back view ..................................................... 37
3.4 DMA-80 EVO internal view ................................................. 37

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4 DMA-80 EVO MILESTONE CONNECT ................. 39
5 SOFTWARE ........................................................... 43
5.1 Login................................................................................... 43
5.2 Operation according to FDA-21 CFR Part 11 ...................... 43
5.3 Login according to CFR 11 ................................................. 44
5.4 Methods release by signature ............................................. 44
5.5 Data file storage and signature ........................................... 44
5.6 Data safety of measurement file in CFR Part 11 ................. 45
5.7 Documentation - erroneous login - ...................................... 45
5.8 Password expiration ........................................................... 45
5.9 Program end/Logout ........................................................... 45
5.10 General Operations Tips ..................................................... 46
5.10.1 Popup menu .................................................................................... 46
5.10.2 File opening, closing, saving and deleting ........................................ 46
5.10.3 Main page DMA-80 EVO Software ................................................... 49
5.10.4 Print ................................................................................................. 51
5.10.5 Automatic printout in PDF ................................................................ 53
5.10.6 Import CSV files ............................................................................... 53
5.10.7 How to import a CSV file .................................................................. 53
5.10.8 Importing CSV formatted data into Excel ......................................... 55
5.11 Method page....................................................................... 57
5.11.1 Methods-Parameters ....................................................................... 58
5.11.2 Create a new method ....................................................................... 60
5.11.3 Methods management ..................................................................... 63
5.12 Calibration page ................................................................. 64
5.12.1 Calibration- Parameters ................................................................... 64
5.12.2 Create a new calibration .................................................................. 69
5.12.3 How to prepare aqueous standard solutions .................................... 76
5.12.4 Reference page ............................................................................... 77
5.13 Measure page ..................................................................... 81
5.13.1 Measurement- Parameters .............................................................. 81
5.13.2 Create a new measurement ............................................................. 91
5.14 System page....................................................................... 93
5.14.1 System- Parameters ........................................................................ 94
5.15 Settings .............................................................................. 96

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5.15.1 Settings-Access ............................................................................... 96
5.15.2 Settings-Settings ............................................................................ 101
5.16 Service ............................................................................. 120
5.16.1 Service-Autosampler...................................................................... 121
5.16.2 Service-Detector ............................................................................ 124
5.16.3 Service-Test .................................................................................. 126
6 MAINTENANCE ................................................... 128
6.1 Consumables replacement ............................................... 128
6.2 Water discharge from the outlet connection ...................... 137
6.3 Sample boat cleaning ....................................................... 138
7 SOFTWARE MESSAGE AND INQUIRIES .......... 139
7.1 Status message ................................................................ 139
7.2 Analytical error messages ................................................ 139
7.3 Technical error messages ................................................ 142
8 TIPS & TECHNIQUES ......................................... 144
8.1 GENERAL RULES ........................................................... 144
8.2 CALIBRATION .................................................................. 144
8.2.1 How to make a standard liquid solution. ........................................... 144
8.2.2 How to store a liquid standard solution ............................................ 145
8.2.3 How to store standard reference materials ....................................... 145
8.3 BLANKS ........................................................................... 145
8.3.1 How to get good blanks ................................................................... 145
8.3.2 Cleaning methods ............................................................................ 146
8.3.3 Tips for a good cleaning ................................................................... 146
8.4 QUALITY CONTROL ........................................................ 147
8.4.1 How to set up a QC program ........................................................... 147
8.4.2 Acceptance Criteria for QC Samples ............................................... 147
8.5 SAMPLE PROCESSING .................................................. 148
8.5.1 Recommendations for preservation of samples ............................... 148
8.5.2 How to clean the DMA-80 boats ...................................................... 148
8.5.3 How to work at ultratrace levels ....................................................... 149
8.5.4 How to work at standard levels (ppb/ppm) ....................................... 151
8.5.5 How to increase the reproducibility .................................................. 153

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8.6 HOW TO WORK WITH UNKNOWN SAMPLES ............... 153
8.6.1 How to work with non-homogeneous samples ................................. 153
8.6.2 How to increase the recovery of difficult samples ............................. 154
8.6.3 How to work with difficult matrices ................................................... 154
8.6.4 How to work with liquid samples in auto-sampler mode, in order to
avoid evaporation. ....................................................................................... 155
8.7 CONDITIONING PROCEDURE FOR NEW CATALYST
TUBE 156
8.7.1 Introduction ...................................................................................... 156
9 21CFR-11 DMA SOFTWARE .............................. 157
9.1 Activation of the 21CFR part 11 ........................................ 157
9.2 Overview of user profiles and access levels ..................... 158
9.3 Declaration of compliance “DMA software” with FDA-21CFR
part 11 ......................................................................................... 161
10 SAFETY DATA SHEET ....................................... 167

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1 SAFETY RULES
1.1 General Information
 Please read carefully this operator manual before switching on the system and follow its
prescriptions with the utmost care.
 In case the system has been delivered with additional accessories, also carefully read the
instruction manuals of such accessories.
 Follow with particular attention the recommended instructions for Service and Maintenance.
 This Operator Manual is part of the delivery, hence must be always kept together with the
instrument on its working place.
 It is imperative that every person operating this system has read and fully understood this
manual.
 The non-observance of the instructions contained herein or improper use may involve
damages/injuries that are not covered by product liability!
 The national ruling and international safety norms in working environment shall in any case be
observed under user’s responsibility.
 It is also user’s responsibility to keep constantly updated all safety norms ruling in the country
or operation.
 All additional safety guidelines of this manual and the safety guidelines on the unit are as well
to be noted.
Information = indicates important information/recommendations for the user.
Attention = means that the non-observance of the warning may cause damages to unit or
properties.
Warning means that the non-observance of the warning may cause injuries, even severe, to
people.
1.2 Safety rules for start-up
 First start-up of the system must exclusively be carried out by authorized and trained service
engineers appointed by Milestone.
1.3 Safety rules in operations
 The DMA-80 EVO device is intended exclusively to measure the mercury content in the
samples. Any different use or any other use is to be considered as improper. The manufacturer
liability will not cover any resulting damages. The risk is exclusively borne by the operator/user.
 The unit must only be operated within the power limits set forth by the technical specifications.
 The unit must be operated exclusively by specially trained people.
 All electrical parts must always be protected from wet and humidity.
 The unit must be kept clean.
 Note all the safety instructions indicated in this manual, that can be treated as accident
prevention regulations, and the general accepted rules to work with devices.

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 Basic rules of chemistry will always apply. The basic rules of chemistry are valid also for handling
chemicals in this unit. Working with chemicals always takes all those safety measures that are
usually required (e.g. lab coat, protective gloves, protective glasses mask, exhausts hood, etc.)
 The user is responsible for the compliance of the above safety instructions with the local
regulations.
1.4 Safety in service operations
 In case of failure or damage of the unit, the intervention of an authorized and trained service
operator must be requested. The unit shall not be used until successful repair is completed.
In case of leakages or defective unit, disconnect immediately the power supply. Inside the unit
there are components with a lethal high voltage (above 1000V).
 Service can only be carried out by authorized and trained service engineers of the company
MLS-Milestone.
 Do not operate the unit if a minimum doubt exists about its trouble-free operation and good
functioning.
 Works on the electrical and electronic components of the system must be carried out exclusively
by specialized electrical/electronic engineers.
 Before every repair – service – installation always make sure that the unit is disconnected from
the power supply line and cannot involuntarily be switched on.
1.5 No manipulation on the unit allowed
 Constructive changes/modifications of the unit and its accessories are forbidden.
1.6 Parts replacement
 In order to ensure the correct functioning of the unit and in the same time the user safety, please
employ only Milestone original spare parts.
1.7 Customer support
 The documents received with the shipment should include also the warranty and the service
report.
 Once performed the installation, please send us the warranty sheet completely filled-in by fax
or e-mail.
 When service call is necessary, please send the completely filled-in service report, with
precise error description.
Before any reparation, (meeting with service), the unit must be cleaned. In case the pollution
endangers the health of our collaborator or restricts the correct functioning of the unit, Milestone
reserves the right to invoice additional costs for the cleaning. Please specify also the used
chemicals in the service report.
 For any additional info, feel free to contact Milestone representative in your country or the
following address: [e-mail]
1.8 Other hints
1.8.1 Safety of operation
 The unit shall not be installed in an explosive, corrosive or conductive atmosphere (dust,
vapours, gas).
 The system must be operated with the original power supply cable supplied together with the
unit.

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 The power supply cable must be in perfect conditions and must not show any damage. Do not
bend the cable. Do not place it over hot surfaces or hang it and protect it from oil. Never
connect the cable if you have wet hands.
 When you connect the unit, check that the voltage and the frequency of power supplies match
with the value indicated on the label place on the back of DMA-80 EVO.
 Never touch parts with live wires. This may cause an electric shock, which can result in serious
injuries or even death.
 The exhaust gases may contain high Hg concentrations, depending on the working
conditions. A proper treatment of the gases is necessary.
 Should a neon tube or a low pressure lamp break, this could increase the Hg mercury in the
DMA-80 EVO. The area must be properly aired.
1.9 Cleaning instructions
 Before any maintenance on the unit and before cleaning it, disconnect the power supply cable.
 Keep the air inlet and outlet openings always free from pollution.
 You can clean the surfaces of the unit with a wet cloth, warm water and a mild household
cleaning detergent, and then wipe dry. Never use solvents.
1.10 Maintenance instructions
 The user shall simply carry out the maintenance instructions described in this user manual.
For all other operations on the unit, a special trained service engineer should be consulted.
 Before opening the device, disconnect it from power and oxygen supply.
 When opening the cover or removing some components, some live parts remain exposed,
while the unit is connected to the power supply.
 Maintenance works on the unit, while it is under tension, shall not be carried out. If such
activities are unavoidable, they must be carried out by a specialist who is familiar with the
hazards involved.
 Any kind of damages to the protective ground inside or outside the unit can endanger the
operator.
1.10.1 Fuses
 In case of a fuse replacement, this part must be replaced following the indications described
in this manual. The fuses below are built in the unit:
Power Supply: 2 x 6,3 A fuses, 250 V for a power supply of 230 V
2 x 10 A fuses, 250 V for a power supply of 115 V
The following fuses shall be replaced only by service:
Heating coil: 1 x 16 A fuse, 250 V (on motherboard)
Electronics: 1 x 2 A fuse, 250 V (on motherboard)

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2 INSTALLATION
2.1 Unpacking and checking list
When unpacking, verify that all parts received
correspond to the packing list.
Carefully cut the tie down straps, as shown in the
next picture. Cut through the packing tape and
open the carton from the top.
Remove the smaller boxes located in the upper
part of the main box and set them aside. They
contain accessories, which will be needed during
the installation of the DMA-80 EVO. Lift the main
box up off of the pallet to expose and facilitate
removal of the DMA-80 EVO.

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Check the information paper which contain some
advices to follow before starting the unit
Provide a bench of at least 150 cm length and 70 cm wide and place the DMA-80 EVO on the bench. The
unit requires sufficient space (approx. 15 cm) on all sides to allow adequate movement of air.
The weight of the DMA-80 EVO is approximately 56 kg; at least 2 people are
needed to remove the unit from its box and place it on the bench.
2.1.1 DMA-80 EVO STANDARD EQUIPMENT
- Option 1
Terminal 660
Usb stick
Pen

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Pen holder
RJ45 cable
- Option 2
Pc software package
Software and drivers installed on CD-ROM and
USB stick
Gateway
USB cable
RJ45 cable

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- Additional parts included in both options
Power cord
Gas carrier tube
Autosampler plate
Mercury trap
Quartz boats pack
Blue accessories box

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2.2 CATALYST AND AMALGAMATOR INSTALLATION
Before working with DMA- components,wear:
-Mask anti dust FPP3;
-Disposables gloves
-
Protection for the shoes.
For safety reason the catalyst and amalgamator are packed separately to the DMA-80 unit so they need
to be installed as first step.
Attention = HOT components
Put the machine on the working table and with
the help of key, open the housing cover.
Release the remaining pressure from the gas
connector on the back side of the unit

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Pull back the horizontal pneumatic actuator in
order to free the catalyst flange.
Loosen the red screw on the spectrophotometer
plate and move far the spectrophotometer arm.
Loosen the two red screws on the slide rails.
It is not necessary to remove completely the
screws

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Pull out the catalyst oven block.
Open the catalyst flange with an hexagonal
screw (you can use the one included in the blue
accessories case).
Take the catalyst marked with label with red line
from the blue accessories case.
This catalyst was used during the QC protocol in
our factory, so we suggest to install it as first.
Wait to connect the amalgamator.
The new catalyst needs to be drained

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Check this catalyst tube has the o-ring at 5 mm
from the catalyst inlet and the silicon joint on the
tip.
Otherwise, take them from the blue accessories
case.
Install the tube, paying attention that it meet
difficulties or friction during the entrance.
Once the new tube has been installed, close the
flange, and push in the catalyst oven block.

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Tightening the red screws.
Check the proper alignment of the horizontal
pneumatic actuator with the catalyst flange.
In case of bad alignment, adjust the two screws
of the cylinder by turning a bit them. The cylinder
will move a bit right or left.

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Connect the gas carrier. See chapter 2.4
Switch on the machine (see chapter 2.3 on how
to set the voltage and connect the controller)
Wait about 20 minutes before to connect the
amalgamator.
During this time you should see the condensate
(bubble of water) gets out from the catalyst tube.
The catalyst is draining.