Miltenyi Biotec UltraMicroscope Blaze User manual

UltraMicroscope Blaze™
User manual

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notwithstanding the foregoing, the owners of the UltraMicroscope Blaze™ may make copies solely for purposes of training personnel in the use and
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UltraMicroscope Blaze™
User manual
Original instructions
Issued: 2021-08
212526.01
Miltenyi Biotec B.V. & Co. KG
Friedrich-Ebert-Straße 68
51429 Bergisch Gladbach
Germany
Phone [phone]
Fax [phone]
[e-mail]
www.miltenyibiotec.com

Read the chapter Important safety information and all
other information in this user manual before using the
instrument
Before using the UltraMicroscope Blaze, read the chapter Important safety information and all other
information contained in this user manual, including any safety and operating instructions. Pay special
attention to all warnings displayed on the instrument. Failure to read and follow these guidelines could lead to
improper or incorrect usage and result in damage to the instrument. Improper usage could also cause severe
personal injury, death, unpredictable results, instrument malfunction, and premature wear to components
shortening the lifetime of the instrument. Such actions may void your warranty. Keep the user manual and any
other safety and operating instructions provided with the instrument in a safe place accessible to all users for
future reference.
If you have a serious concern regarding the safe use of your instrument, contact your authorized Miltenyi Biotec
service provider or call Miltenyi Biotec Technical Support.
4

Content
1 Important safety information 7
2 Introduction 17
2.1 Introduction 17
2.2 Intended use 17
3 Installation 21
3.1 Components 21
3.2 Accessories 22
3.3 Consumables 22
3.4 Unpacking and installation 23
4 Switching on and off 25
4.1 Switching on the instrument 25
4.2 Switching off the instrument 25
4.3 The interface of the UltraMicroscope Blaze 25
5 Preparing the instrument 27
6 Laser alignment 29
6.1 Light sheet alignment 29
6.2 Setting the beam path 29
6.3 Adjustment of the lateral beams on the right-hand side 34
6.4 Adjustment of the center beam on the left-hand side 34
6.5 Adjustment of the lateral beams on the left-hand side 35
7 Setting up an experiment 37
7.1 Capturing a single-color z-stack 37
7.1.1 Aligning the sample 37
7.1.2 Optimizing the measurement settings 39
7.1.3 Capturing an image 40
7.2 Capturing a multi-color z-stack 40
7.3 Capturing a mosaic image 42
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8 Maintenance 45
8.1 Servicing 45
8.2 Cleaning the instrument 45
8.3 Cleaning the cuvette 45
9 Technical data and specifications 47
9.1 Specifications 47
9.2 EC / EU Declaration of Conformity 49
10 Technical support 51
11 Legal notes 53
11.1 Limited warranty 53
11.2 Trademarks 54
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IMPORTANT SAFETY INFORMATION
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1
Important safety information
General safety instructions
THIS CHAPTER DESCRIBES POTENTIALLY HAZARDOUS SITUATIONS ASSOCIATED WITH THIS INSTRUMENT AND
PROVIDES IMPORTANT SAFETY INFORMATION TO MINIMIZE THE RISKS AND PROTECT YOURSELF AND OTHERS.
The instrument can be used safely, if the following is ensured:
The instrument is installed correctly.
The instrument is only operated by professional laboratory personnel.
General safety practices are followed.
All instructions in this user manual are followed.
• Use this instrument only as indicated in this user manual to avoid injury to personnel and damage of the
equipment.
• Keep this user manual in a place that is always accessible to all users.
• Follow local working area safety instructions and laboratory policies and standards for health, safety, and
prevention of accidents.
• Ensure safe working conditions and provide for sufficient light.
• Ensure that the power switch and the plug of the power cable of the instrument are easily accessible.
• To entirely disconnect the instrument from the power supply, unplug the power cable.
• Ensure that the power cable plug is close to the user.
• If it is necessary to cut the power supply, unplug the cable from the power outlet and contact an authorized
Miltenyi Biotec service provider or Miltenyi Biotec Technical Support.
• Read the instructions in the chapter Important safety information before using the instrument.
• Check the instrument before each use for visible damage.
• In case of severe accidents, damages to the instrument, or if smoke or flames appear, cut the power supply
immediately.
• Do not use the instrument if it has been dropped or is damaged. Contact Miltenyi Biotec Technical Support
immediately.

Hazard levels
Signal words are used to warn against hazardous situations and property damages. The following signal words
are used in this user manual.
or WARNING! indicates a hazardous situation that could result in serious injury or death.
or CAUTION! indicates a hazardous situation that could result in minor or moderate injury. It is also used
to warn against unsafe practices.
or NOTICE indicates information considered important but not hazard related (e.g. messages relating to
property damage).
Safety symbols
SAFETY WARNING: THE DOCUMENTATION MUST BE CONSULTED IN ALL CASES WHERE THIS SAFETY
SYMBOL IS USED ON THE INSTRUMENT
ELECTRICITY
MOVING PARTS
SHARP ELEMENTS
HAZARDOUS LASER RADIATION
BIOLOGICAL HAZARD
CRUSH HAZARD
ERGONOMIC HAZARD
TRIP HAZARD
WEEE (WASTE OF ELECTRICAL AND ELECTRONIC EQUIPMENT)
READ THE USER MANUAL BEFORE USING THE INSTRUMENT
ON (POWER ON)
OFF (POWER OFF)
WEIGHT
SERIAL NUMBER
TYPE NUMBER
ORDER NUMBER
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MANUFACTURER
EUROPEAN CONFORMITY APPROVAL
Safety labels
Notice the hazard points and safety symbols of the instrument.
• Keep safety labels and safety markings clean and legible.
• Inspect the safety labels and safety markings regularly and replace them if they are not legible or identifiable
from a safe viewing distance.
• Contact Miltenyi Biotec for replacement labels.
Figure 1.1: Hazard areas and safety symbols on the front side of the instrument.
Figure 1.2: Hazard areas and safety symbols on the rear side of the instrument.

Figure 1.3: Hazard areas and safety symbols on the left-hand side of the instrument.
Figure 1.4: Hazard areas and safety symbols on the right-hand side of the instrument.
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Electricity and fire hazards
ELECTRICITY
Electric devices bear the risk of electric shock, short circuits, overheating, fire, and explosion.
This may lead to burns, severe personal injury, or death.
Protective housing
The housing of the instrument reduces the risk of an electric shock and short circuits.
• Do not disassemble the instrument.
• Do not use the instrument if it has been dropped or is damaged.
Cables and power supplies
The instrument has a three-wire electrical grounding plug with a third pin for grounding. Using the instrument
with other than the supplied cables is potentially hazardous.
• Only use the included power cable.
• Do not use the instrument if the power cable is damaged.
• Only insert the plug into a grounded power outlet. Do not force the plug into an incorrect electrical outlet.
• Operate the instrument only from a power source that meets the electrical specifications mentioned on the
marking plate. If you have questions about the type of power source to use, contact the Miltenyi Biotec
Technical Support or your local electricity supplier.
Liquids inside the instrument
Liquids inside the instrument can cause short circuits.
• Do not use the instrument if liquids have entered the inside of it.
• Protect the instrument against spilled liquids.
• Do not allow liquids to enter the inside of the instrument.
• Clean up spilled liquids immediately.
• Unplug the instrument before cleaning it.
• Use only small amounts of cleaning agents on a soft cloth to wipe the instrument. Do not spray or pour liquid
cleaning agents onto or into the instrument.
Humid and dusty environments
A change in the environmental temperature may cause condensation inside the instrument. Humidity,
condensation, or dust inside the instrument can cause short circuits.
• Use the instrument only indoors.
• Do not use the instrument in a wet location or areas with high humidity or condensation.
• After moving the instrument from a cold environment to room temperature, wait for the instrument to
dehumidify before using it.

Mechanical hazards
MOVING PARTS
Risk of crushing or cutting.
Motorized turret
The motorized turret moves while the instrument is in operation.
• Keep away from the motorized turret while the instrument is in operation.
• Do not obstruct the movement of the motorized turret.
SHARP ELEMENTS
Risk of cutting.
UltraMicroscope Blaze
• Do use caution while handling the instrument.
• Provide sufficient lighting and workspace.
Optical radiation hazards
LASER BEAM
Exposure to the laser may cause eye injury.
Laser beam combiner
The instrument is equipped with a Class 3B laser product (Laser BC) or Class 4 laser product (Supercontinuum
WLL) per standard IEC 60825-1:2014.
• Do not remove the protective housing.
• Do not look directly at laser or reflected laser radiation from a mirrored surface.
• Do not insert reflective objects into the cuvette.
• Switch off immediately, if there is any indication of laser radiation outside the instrument.
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Biological hazards
BIOLOGICAL HAZARD
Contamination or infection may lead to severe personal injury or death, depending on the used
material.
Biological material
All biological material must be considered potentially infectious.
• Wear personal protective equipment (such as gloves, safety glasses, etc.) as indicated in the documentation
for the particular substance.
• If hazardous or potentially infectious material has been spilled or leaked from the system, decontaminate the
affected area.
• Operate the instrument in a biological safety cabinet suitable for the used specimen if hazardous or potentially
infectious materials are processed.
• Take care when handling samples and reagents.
• Provide sufficient lighting and workspace.
Waste
All liquid and solid waste must be considered hazardous.
• Follow local regulations regarding waste disposal.
Equipment damage
Defective or inadequate equipment can cause a biological hazard. Some decontamination methods may
damage the instrument.
• If parts of the instrument are damaged, unplug and do not use the instrument until damaged parts are
replaced. Contact Miltenyi Biotec Technical Support, if necessary.
• Follow the instructions in the datasheet for each consumable.
• Use only 70% ethanol or isopropyl alcohol for disinfection.
Chemical hazards
CHEMICAL HAZARD
Substances and reagents can be hazardous.
Hazardous substances and reagents
• All safety measures in section Biological hazards also apply to any hazardous substances and reagents that
may be present in the sample.
• Use any substances and reagents only as stated in the respective data sheet.
• Operate the instrument in a fume hood or with a fume extraction arm if hazardous substances and reagents
are processed.

Ergonomic hazards
CRUSH HAZARD
Risk of crushing.
Heavy instrument
• Only use tables that support a weight of 250 kg.
• Do not install the instrument yourself.
• Only trained Miltenyi Biotec personnel or personnel authorized by Miltenyi Biotec is allowed to install the
instrument.
ERGONOMIC HAZARD
Risk of tearing or straining muscles.
Heavy instrument
• Do not move the instrument yourself.
• Only trained Miltenyi Biotec personnel or personnel authorized by Miltenyi Biotec is allowed to transport the
instrument.
Environmental hazards
TRIP HAZARD
Tripping can cause injuries.
Uncovered cables
• Provide sufficient lighting and workspace.
• Check the environment around the instrument before each use for trip hazards such as cables lying on the
floor.
Servicing and transportation
Servicing
Improper servicing or repair of the instrument or use of unauthorized parts can cause malfunction of or damage
to the instrument. This can cause hazards to users. Unless otherwise specifically noted in this user manual or
other Miltenyi Biotec documentation, do not service the instrument yourself. Servicing and repair must be
performed by Miltenyi Biotec certified and qualified service personnel. If the instrument needs servicing,
decontaminate the instrument to remove any hazardous material. If you have questions regarding proper
decontamination or shipment, contact Miltenyi Biotec Technical Support for assistance.
Transportation
The instrument should be transported with care in packaging specified by Miltenyi Biotec. Internal damage can
occur if the instrument is subjected to excessive vibration or if it is dropped. If the instrument needs to be
shipped back to the manufacturer for service, contact Miltenyi Biotec for instructions and packaging materials.
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Disposal
WASTE OF ELECTRICAL AND ELECTRONIC EQUIPMENT (WEEE) CUSTOMER INFORMATION
Dispose of your end-of-life Miltenyi Biotec products in accordance with the applicable WEEE and hazardous
waste disposal legislation, which may differ by country or region. Electrical equipment may contain hazardous
substances that may have a serious detrimental effect on the environment and/or human health. All equipment
must be specifically collected and treated by designated waste facilities and by qualified WEEE compliance
schemas. By ensuring that you dispose of your unwanted electrical and electronic equipment according to the
applicable WEEE and hazardous waste disposal legislation, you are helping to preserve our natural resources and
protect human health. Miltenyi Biotec is committed to protecting the environment. Miltenyi Biotec offers
product end-of-life return programs in many countries, and partners with licensed WEEE compliance schemes
throughout the world. Miltenyi Biotec takes back your end-of-life Miltenyi Biotec equipment for recycling free of
charge. The terms and availability of this offer vary by geography because of differences in regulatory
requirements. Note that, depending on the type and use of your equipment, additional requirements may
apply. Before shipping the instrument back to the manufacturer for disposal, decontaminate the instrument to
remove any hazardous material. For more information, or if you wish to dispose of your end-of-life Miltenyi
Biotec equipment, contact your local Miltenyi Biotec representative or Miltenyi Biotec Technical Support.

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2
INTRODUCTION
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2
Introduction
2.1 Introduction
The UltraMicroscope Blaze is a fully automated light sheet microscope for imaging cleared tissue samples with a
subcellular resolution.
2.2 Intended use
The UltraMicroscope Blaze is a laboratory equipment for fluorescence microscopic analysis of cleared samples
up to a size of 35 mm × 60mm × 17 mm. The sample may only be examined in the supplied cuvette.
Unauthorized modifications of the cuvette or the instrument as a whole are not permitted for safety reasons.
The cuvette may only be filled with MACS Imaging Solution or another microscopy solution approved by
Miltenyi Biotec. The operating, maintenance, and servicing instructions described in this manual must be
observed.
The UltraMicroscope Blaze is for research use only in a laboratory environment. It is to be used by laboratory
professional users only.
Figure 2.1: Front view of the UltraMicroscope Blaze.

Figure 2.2: Rear view of the UltraMicroscope Blaze.
Figure 2.3: Detail view of the I/O ports and power switch.
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INTRODUCTION
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2
INTRODUCTION
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Figure 2.4: Jog wheel for moving the sample arm in 3 dimensions (x, y, z) and sensitivity.

2
INTRODUCTION
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