Optika Italy ECOVISION Series User manual

Ver. 2.0 2020
Model
B-20R
B-20CR
M-100FX
M-100FLed
ECOVISION Series
INSTRUCTION MANUAL

Page 2
Summary
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Instrument description 4
5.1 B-20R 4
5.2 B-20CR 5
5.3 M-100FX 6
5.4 M-100FLed 7
6. Unpacking 8
7. Assembling 8
7.1 Assembling the microscope 8
7.1.1 Installing the batteries (M-100FLed) 8
8. Use of the microscope 9
8.1 Switching on the microscope 9
8.1.1 B-20R / B-20CR / M-100FX 9
8.1.2 M-100FLed 9
8.2 Light intensity adjustment 9
8.2.1 M-100FX 9
8.2.2 B-20R / B-20CR / M-100FX 9
8.2.3 M-100FLed 9
8.3 Placing the specimen 10
8.3.1 B-20R / M-100FX / M-100FLed 10
8.3.2 B-20CR 10
8.4 Use with batteries 10
8.4.1 M-100FLed 10
8.5 Aperture diaphragm 11
9. Maintenance 12
10. Troubleshooting 13
Equipment disposal 14

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
OBJECTIVE
NOSEPIECE
STAGE
EYEPIECE
FINE FOCUSING KNOB
COARSE
FOCUSING KNOB
MAIN BODY
ILLUMINATOR
LOCK SCREW
OBSERVATION HEAD
CARRYING HANDLE
SLIDE CLAMP
CONDENSER
5. Instrument description
5.1 B-20R

Page 5
NOSEPIECE
OBJECTIVE
STAGE
STAGE KNOBS
CONDENSER
EYEPIECE
COARSE FOCUSING
KNOB
FINE FOCUSING
KNOB
MAIN BODY
APERTURE
DIAPHRAGM
ILLUMINATOR
LOCK SCREW
OBSERVATION HEAD
CARRYING HANDLE
SLIDE CLAMP
5.2 B-20CR

Page 6
OBJECTIVE
CONDENSER
ILLUMINATOR
EYEPIECE
MAIN BODY
OBSERVATION
HEAD
FINE FOCUSING
KNOB
COARSE
FOCUSING KNOB
STAGE
SLIDE CLAMP
NOSEPIECE
5.3 M-100FX
APERTURE
DIAPHRAGM
LOCK SCREW

Page 7
OBJECTIVE
ILLUMINATOR
CONDENSER
ON-OFF /
BRIGHTNESS
ADJUSTMENT KNOB
EYEPIECE
MAIN BODY
OBSERVATION
HEAD
FINE FOCUSING
KNOB
COARSE
FOCUSING KNOB
STAGE
SLIDE CLAMP
NOSEPIECE
5.4 M-100FLed
APERTURE
DIAPHRAGM
LOCK SCREW

Page 8
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
7.1 Assembling the microscope
• No installation procedure is requested for this mi-
croscope series.
• Place the microscope on a sturdy table and con-
nect the power supply.
7.1.1 Installing the batteries (M-100FLed)
1. Using a screwdriver remove the xing screw of
the battery compartment cover. (Fig. 1)
2. Remove the cover (Fig. 2) and insert the batteries
(provided), respecting the polarity. (Fig. 3)
3. Put back the battery compartment cover and
screw the xing screw again.
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3

Page 9
8. Use of the microscope
8.1 Switching on the microscope
8.1.1 B-20R / B-20CR / M-100FX
1. Operate on the main switch ① placed in the rear
side of the microscope, moving the selector on
“I”. (Fig. 4)
8.1.2 M-100FLed
1. Operate on the main switch ② placed in front of
the microscope. (Fig. 5)
8.2 Light intensity adjustment
8.2.1 M-100FX
• No light intensity adjustment can be done on this
model.
8.2.2 B-20R / B-20CR / M-100FX
1. Turn the light intensity dial ③ placed on the right
side of the microscope. (Fig. 6)
8.2.3 M-100FLed
1. Operate on the main switch ② placed in front of
the microscope. (Fig. 5)
FFig. 4ig. 4
FFig. 5ig. 5
FFig. 6ig. 6
③
①
②

Page 10
8.3 Placing the specimen
8.3.1 B-20R / M-100FX / M-100FLed
1. Place the specimen on the stage and secure it
using the stage clips ①. (Fig. 7).
8.3.2 B-20CR
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverslide 0,17 mm. (Fig. 8)
1. Open the spring arm of the slide holder ②and
place the slide from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could
cause the falling of the slide.
8.4 Use with batteries
8.4.1 M-100FLed
• The microscope is provided with an ON-OFF
switch ③ (Fig. 9) placed on the bottom side of the
microscope. The use of the switch is as follows:
1. Switch placed in “OFF” position: use of the
microscope is possible only with external power
supply. Battery use and recharge is disabled.
2. Switch placed in “ON” position: use of the mi-
croscope is possible either with external power
supply or with batteries. In this case the battery
recharge is enabled.
FFig. 7ig. 7
FFig. 9ig. 9
③
①
FFig. 10ig. 10
FFig. 8ig. 8
②

Page 11
8.5 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the ap-
erture diaphragm affects the image contrast. In-
creasing or reducing this value one can vary res-
olution, contrast and depth of focus of the image.
Move the diaphragm ring ① (Fig. 10) on the value
corresponding to the objective in use. In this case
the optimal setting of the condenser is achieved.
• With low contrast specimens set the numerical
aperture to about 70%-80% of the objective’s
N.A. If necessary, remove on eyepiece and, look-
ing into empty sleeve, adjust the condenser’s dia-
phragm in order to obtain an image like the one
in Fig. 11.
FFig. 11ig. 11
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 10ig. 10
①

Page 12
9. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of
5°-40°C and a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no
moving parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn off the light immediately, cover the microscope with the provided dust-cover,
and keep it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region
matches with the operation voltage of the equipment and that the lamp switch is in off-
position.
• Users should observe all safety regulations of the region. The equipment has acquired the
CE safety label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sufcient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near
sparks or near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 13
10. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Batteries discharged Charge the batteries
Dirt or dust is visible in the eld of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped down
too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an incorrect
position Move the nosepiece to a click stop
Aperture diaphragm is too closed or to
open Adjust aperture diaphragm
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly
For transmitted light observation, the
coverglass thickness must not exceed
0.17mm
Use a coverglass with thickness
0.17mm
Focus is not even Slide holder is not flat. Move the speci-
men to a flat position
One side of the image is out of focus. The nosepiece is not in the center of
the light path Turn the nosepiece to a click stop
The specimen is out of place (tilted) Place the specimen at on the stage.
The optical performance of the sample
cover glass is poor Use a cover glass of better quality
II. Electric Section:
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord

Page 14
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste. The separate collection of this equipment at the end of its lifetime is or-
ganized and managed by the producer. The user will have to contact the manufacturer and follow the rules that
he adopted for end-of-life equipment collection. The collection of the equipment for recycling, treatment and
environmentally compatible disposal, helps to prevent possible adverse effects on the environment and health
and promotes reuse and/or recycling of materials of the equipment. Improper disposal of the product involves
the application of administrative penalties as provided by the laws in force.

OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: [phone]
[email protected] - www.optikamicroscopes.com
OPTIKA Spain
[email protected]
OPTIKA USA
[email protected]
OPTIKA China
[email protected]
OPTIKA India
[email protected]
OPTIKA Central America
[email protected]
®
®
®
®
®
®

Ver. 2.0 2020
Modello
B-20R
B-20CR
M-100FX
M-100FLed
Serie ECOVISION
MANUALE DI ISTRUZIONI

Pagina 17
Sommario
1. Avvertenza 18
2. Simboli 18
3. Informazioni sulla sicurezza 18
4. Utilizzo previsto 18
5. Descrizione dello strumento 19
5.1 B-20R 19
5.2 B-20CR 20
5.3 M-100FX 21
5.4 M-100FLed 22
6. Disimballaggio 23
7. Assemblaggio 23
7.1 Procedura di assemblaggio 23
7.1.1 Montaggio delle batterie (M-100FLed) 23
8. Uso del microscopio 24
8.1 Accensione del microscopio 24
8.1.1 B-20R / B-20CR / M-100FX 24
8.1.2 M-100FLed 24
8.2 Regolazione dell’intensità luminosa 24
8.2.1 M-100FX 24
8.2.2 B-20R / B-20CR / M-100FX 24
8.2.3 M-100FLed 24
8.3 Posizionare il campione 25
8.3.1 B-20R / M-100FX / M-100FLed 25
8.3.2 B-20CR 25
8.4 Uso con batterie 25
8.4.1 M-100FLed 25
8.5 Diaframma di apertura 26
9. Manutenzione 27
10. Risoluzione dei problemi 28
Smaltimento 29

Pagina 18
1. Avvertenza
Questo microscopio è uno strumento scientico di alta precisione, progettato per durare a lungo con una minima
manutenzione; la realizzazione è secondo i migliori standard ottici e meccanici, per poter essere utilizzato
quotidianamente. Vi ricordiamo che questo manuale contiene informazioni importanti per la sicurezza e per la
manutenzione dello strumento, e deve quindi essere messo a disposizione di coloro che lo utilizzeranno.
Decliniamo ogni responsabilità derivante da un utilizzo dello strumento non indicato nel presente manuale.
2. Simboli
La seguente tabella riporta i simboli utilizzati in questo manuale.
PERICOLO
Questo simbolo indica un rischio potenziale ed avverte di procedere con cautela.
SHOCK ELETTRICO
Questo simbolo indica un rischio di shock elettrico
3. Informazioni sulla sicurezza
Per evitare shock elettrici
Prima di collegare il cavo di alimentazione alla presa elettrica, assicurarsi che il voltaggio della rete locale
coincida con il voltaggio dello strumento e che l’interruttore dell’illuminazione sia nella posizione “OFF”.
Gli utenti dovranno seguire tutte le norme di sicurezza locali. Lo strumento è certicato CE. In ogni caso, gli
utilizzatori sono gli unici responsabili per un utilizzo sicuro dello strumento. Per l’utilizzo in sicurezza dello
strumento è importante attenersi alle seguenti istruzioni e leggere il manuale in tutte le sue parti.
4. Utilizzo previsto
Modelli standard
Solo per applicazioni di ricerca ed usi didattici. Non indicato per utilizzo diagnostico e terapeutico umano e ve-
terinario.
Modelli IVD
Anche per uso diagnostico, nalizzato ad ottenere informazioni sulla situazione siologica o patologica del sog-
getto.

Pagina 19
OBIETTIVI
REVOLVER
TAVOLINO
OCULARE
MANOPOLA
MICROMETRICA
MANOPOLA
MACROMETRICA
STATIVO
ILLUMINATORE
VITE DI BLOCCO
TESTA DI OSSERVAZIONE
MANIGLIA DI
TRASPORTO
MOLLETTINE
CONDENSATORE
5. Descrizione dello strumento
5.1 B-20R

Pagina 20
MANOPOLE
TAVOLINO
OCULARE
MANOPOLA
MACROMETRICA
MANOPOLA
MICROMETRICA
DIAFRAMMA DI
APERTURA
ILLUMINATORE
VITE DI BLOCCO
5.2 B-20CR
TESTA DI OSSERVAZIONE
MANIGLIA DI
TRASPORTO
STATIVO OBIETTIVI
REVOLVER
TAVOLINO
MOLLETTINE
CONDENSATORE