MO BIO Laboratories PowerWater DNA User manual

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PowerWater®DNA Isolation Kit
(For isolation of genomic DNA from membrane filtered water samples)
Catalog No.
Quantity
Filters
14900-50-NF
50 Preps
No filters
14900-100-NF
100 Preps
No filters
Instruction Manual
Inhibitor Removal Technology®(IRT) is a registered trademark of MO BIO Laboratories, Inc. and is covered by US patent
protection as well as international patents pending.
Please recycle
Version: 01052012

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Table of Contents
Introduction .................................................................................................................................... 3
Protocol Overview........................................................................................................................... 3
Flow Chart ………........................................................................................................................... 4
Equipment Required ...................................................................................................................... 5
Kit Contents & Storage .................................................................................................................. 5
Precautions & Warnings ................................................................................................................ 5
Important Notes Before Starting......................................................................................................5
Protocols:
Experienced User Protocol...................................................................................................6
Detailed Protocol (Describes what is happening at each step) ...........................................8
Vacuum Manifold Protocol .................................................................................................11
Hints & Troubleshooting Guide ......................................................................................................13
Contact Information ........................................................................................................................15
Other Quality Products Available....................................................................................................16

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Introduction
The PowerWater®DNA Isolation Kit can isolate genomic DNA from a variety of filtered water samples.
Utilizing our patented Inhibitor Removal Technology®(IRT), even water containing heavy amounts of
contaminants can be processed to provide DNA of high quality and yield. The kit can isolate DNA
equally as well from any commonly used filter membrane type. This kit differs from our UltraClean®
Water DNA Isolation Kit by the addition of a novel bead tube with an optimized bead mix, a reformulated
lysis buffer, IRT technology, and the reduction in sample volume so that nearly all processing occurs in a
microcentrifuge. Purified DNA is ready to use in a final 100 µl elution volume.
Protocol Overview
The PowerWater®DNA Isolation Kit starts with the filtration of a water sample onto a filter membrane.
Filter membranes can be purchased separately from MO BIO or can be user supplied. MO BIO filter
membranes are sterile, disposable, and easy to use. The membrane is then added to our special 5 ml
bead beating tube containing a unique bead mix. Rapid and thorough lysis occurs through vortex mixing
in a reformulated lysis buffer that enhances the isolation of microorganisms from filter membranes. After
the protein and inhibitor removal steps, total genomic DNA is captured on the MO BIO Laboratories silica
spin column. High quality DNA is then washed and eluted from the spin column membrane for use in
downstream applications including PCR and qPCR.
This kit is for research purposes only. Not for diagnostic use.
Other Related Products
Catalog No.
Quantity
Vortex Adapter for Vortex Genie®2
13000-V1-15
13000-V1-5
Holds 4 (5 ml or 15 ml) Tubes
Holds 6 (5 ml) Tubes
Water Filter Adapter
14800-10-WFA
1
Water Filter (0.45 µm)
14800-10-WF
14800-25-WF
14800-50-WF
14800-100-WF
10 units
25 units
50 units
100 units
Water Filter (0.22 µm)
14880-10-WF
14880-25-WF
14880-50-WF
14880-100-WF
10 units
25 units
50 units
100 units
Vortex Genie®2 Vortex
13111-V-220
13111-V
1 unit (220V)
1 unit (120V)
PCR Water (Certified DNA-free)
17000-1
17000-5
17000-10
17000-11
1 ml
5 x 1 ml
10 x 1 ml
10 ml bottle
RapidWater™ DNA Isolation Kit
14810-50-NF
14810-100-NF
50 preps (No filters)
100 preps (No filters)
PowerVac™ Manifold
11991
1 manifold
PowerVac™ Mini System
11992
1 unit + 20 adapters
PowerVac™ Mini Spin Filter Adapters
11992-10
11992-20
10 adapters
20 adapters

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Equipment Required
Centrifuge for 15 ml tubes (≤4000 x g)
Disposable/reusable filter funnels
Filter membranes (if using a reusable filter
funnel)
Microcentrifuge (13,000 x g)
Pipettors
Vortex
Vortex Adapter
Vacuum Filtration System
Kit Contents
Kit Catalog#
14900-50-NF
Kit Catalog#
14900-100-NF
Component
Amount
Amount
PowerWater®Bead Tubes
50 tubes
100 tubes
Solution PW1
55 ml
110 ml
Solution PW2
11 ml
22 ml
Solution PW3
2 x 18 ml
3 x 24 ml
Solution PW4
2 x 18 ml
3 x 24 ml
Solution PW5
2 x 18 ml
3 x 24 ml
Solution PW6
5.5 ml
11 ml
Spin Filters
50
100
2 ml Collection Tubes
250
500
Kit Storage
Store all reagents and kit components at room temperature (15-30°C).
Precautions
Please wear gloves when using this product. Avoid all skin contact with kit reagents. In case of contact,
wash thoroughly with water. Do not ingest. See Material Safety Data Sheets for emergency procedures
in case of accidental ingestion or contact. All MSDS information is available upon request (760-929-
9911) or at www.mobio.com. Reagents labeled flammable should be kept away from open flames and
sparks.
WARNING: Solutions PW3, PW4 and PW5 contain alcohol. They are flammable.
Important Notes Before Starting:
Solution PW1 must be warmed at 55°C for 5-10 minutes to dissolve precipitates prior to use. Solution
PW1 should be used while still warm.
Solution PW3 may precipitate over time. If precipitation occurs, warm at 55°C for 5-10 minutes. Solution
PW3 can be used while still warm.
Shake to mix Solution PW4 before use.

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Experienced User Protocol
Please wear gloves at all times
Warm Solution PW1 prior to use at 55°C for 5-10 minutes. Use Solution PW1 while still warm.
Check Solution PW3 and warm at 55°C for 5-10 minutes if necessary. Solution PW3 can be used
while still warm.
1. Filter water samples using a reusable or disposable filter funnel attached to a vacuum source.
Disposable filter funnels, containing 0.22 µm or 0.45 µm filter membranes, can be ordered from
MO BIO Laboratories (see page 3). The volume of water filtered will depend on the microbial
load and turbidity of the water sample. (Please see Types of Water Samples in the Hints and
Troubleshooting Guide section of the Instruction Manual).
2. If using a reusable filter funnel, remove the upper portion of the apparatus. If using a MO BIO
Laboratories filter funnel, remove the 100 ml upper portion of the filter cup from the catch
reservoir by snapping it off.
3. Using two sets of sterile forceps, pick up the white filter membrane at opposite edges and roll the
filter into a cylinder with the top side facing inward.
Note: Do not tightly roll or fold the filter membrane. To see a video of this technique,
please visit the PowerWater® DNA Isolation Kit product page on www.mobio.com.
4. Insert the filter into the 5 ml PowerWater®Bead Tube.
5. Add 1 ml of Solution PW1 to the PowerWater®Bead Tube.
Note: Solution PW1 must be warmed to dissolve precipitates prior to use. Solution PW1
should be used while still warm. For samples containing organisms that are difficult to
lyse (fungi, algae) an additional heating step can be included. See Alternate Lysis
Method in the Hints and Troubleshooting Guide.
6. Secure the PowerWater®Bead Tube horizontally to a MO BIO Vortex Adapter, catalog number
13000-V1-15 or 13000-V1-5.
7. Vortex at maximum speed for 5 minutes.
8. Centrifuge the tubes ≤ 4000 x g for 1 minute at room temperature. The speed will depend on the
capability of your centrifuge. (This step is optional if a centrifuge with a 15 ml tube rotor is
not available, but will result in minor loss of supernatant).
9. Transfer all the supernatant to a clean 2 ml Collection Tube (provided). Draw up the supernatant
using a 1 ml pipette tip by placing it down into the beads.
Note: Placing the pipette tip down into the beads is required. Pipette more than once to
ensure removal of all supernatant. Any carryover of beads will not affect subsequent
steps. Expect to recover between 600-650 µl of supernatant depending on the type of
filter membrane used.
10. Centrifuge at 13,000 x g for 1 minute.
11. Avoiding the pellet, transfer the supernatant to a clean 2 ml Collection Tube (provided).
12. Add 200 µl of Solution PW2 and vortex briefly to mix. Incubate at 4°C for 5 minutes.
13. Centrifuge the tubes at 13,000 x g for 1 minute.
14. Avoiding the pellet, transfer the supernatant to a clean 2 ml Collection Tube (provided).
15. Add 650 µl of Solution PW3 and vortex briefly to mix.
Note: Check Solution PW3 for precipitation prior to use. Warm if necessary. Solution
PW3 can be used while still warm.
16. Load 650 µl of supernatant onto a Spin Filter and centrifuge at 13,000 x g for 1 minute. Discard
the flow through and repeat until all the supernatant has been loaded onto the Spin Filter.
Note: A total of two loads for each sample processed are required.
17. Place the Spin Filter basket into a clean 2 ml Collection Tube (provided).

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18. Shake to mix Solution PW4 before use. Add 650 µl of Solution PW4 and centrifuge at 13,000 x g
for 1 minute.
19. Discard the flow through and add 650 µl of Solution PW5 and centrifuge at 13,000 x g for 1
minute.
20. Discard the flow through and centrifuge again at 13,000 x g for 2 minutes to remove residual
wash.
21. Place the Spin Filter basket into a clean 2 ml Collection Tube (provided).
22. Add 100 µl of Solution PW6 to the center of the white filter membrane.
23. Centrifuge at 13,000 x g for 1 minute.
24. Discard the Spin Filter basket. The DNA is now ready for any downstream application. No
further steps are required.
We recommend storing the DNA frozen (-20°C to -80°C). Solution PW6 contains no EDTA. To
concentrate the DNA, see the Hints and Troubleshooting Guide.
Thank you for choosing the PowerWater®DNA Isolation Kit!

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Detailed Protocol (Describes what is happening at each step)
Please wear gloves at all times
Warm Solution PW1 prior to use at 55°C for 5-10 minutes. Use Solution PW1 while still warm.
Check Solution PW3 and warm at 55°C for 5-10 minutes if necessary. Solution PW3 can be used
while still warm.
1. Filter water samples using a reusable or disposable filter funnel attached to a vacuum source.
Disposable filter funnels, containing 0.22 µm or 0.45 µm filter membranes, can be ordered from
MO BIO Laboratories (see page 3). The volume of water filtered will depend on the microbial
load and turbidity of the water sample. (Please see Types of Water Samples in the “Hints and
Troubleshooting Guide”section of Instruction Manual).
What’s happening: A reusable or disposable filter funnel is attached to a vacuum filtration system.
Microorganisms are trapped on top of and within the filter membrane.
2. If using a reusable filter funnel, remove the upper portion of the apparatus. If using a MO BIO
Laboratories filter funnel, remove the 100 ml upper portion of the filter cup from the catch
reservoir by snapping it off.
3. Using two sets of sterile forceps, pick up the white filter membrane at opposite edges and roll the
filter into a cylinder with the top side facing inward.
Note: Do not tightly roll or fold the filter membrane. To see a video of this technique,
please visit the PowerWater®DNA Isolation Kit product page on www.mobio.com.
4. Insert the filter into the 5 ml PowerWater®Bead Tube.
What’s happening: Loosely rolling and inserting the filter membrane into the PowerWater®Bead Tube
allows for efficient bead beating and homogenization in proceeding steps.
5. Add 1 ml of Solution PW1 to the PowerWater®Bead Tube.
Note: Solution PW1 must be warmed to dissolve precipitates prior to use. Solution PW1
should be used while still warm. For samples containing organisms that are difficult to
lyse (fungi, algae) an additional heating step can be included. See Alternate Lysis
Method in the “Hints and Troubleshooting Guide”.
What’s happening: Solution PW1 is a strong lysing reagent that includes a detergent to help break cell
walls and will remove non-DNA organic and inorganic material.It is also part of the patented Inhibitor
Removal Technology® (IRT). When cold, this solution will form a white precipitate in the bottle. Heating to
55°C will dissolve the components without harm. Solution PW1 should be used while it is still warm.
6. Secure the PowerWater®Bead Tube horizontally to a MO BIO Vortex Adapter, catalog number
13000-V1-15 or 13000-V1-5.
7. Vortex at maximum speed for 5 minutes
What’s happening: The mechanical action of bead beating will break apart the surface of the filter
membrane that contains trapped cells and aids in cell lysis. Use of the vortex adapter will maximize

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homogenization by holding the tubes equal distance and angle from the center of rotation. Avoid using
tape, which can become loose and result in reduced homogenization efficiency.
8. Centrifuge the tubes ≤ 4000 x g for 1 minute at room temperature. The speed will depend on the
capability of your centrifuge. (This step is optional if a centrifuge with a 15 ml tube rotor is
not available, but will result in minor loss of supernatant).
9. Transfer the supernatant to a clean 2 ml Collection Tube (provided). Draw up the supernatant
using a 1 ml pipette tip by placing it down into the beads.
Note: Placing the pipette tip down into the beads is required. Pipette more than once to
ensure removal of all supernatant. Any carryover of beads will not affect subsequent
steps. Expect to recover between 600-650 µl of supernatant depending on the type of
filter membrane used.
What’s happening: The supernatant is separated and removed from the filter membrane and beads at this
step.
10. Centrifuge at 13,000 x g for 1 minute.
What’s happening: Any remaining beads, proteins, and cell debris are removed at this step. This step is
important for removal of any remaining contaminating non-DNA organic and inorganic matter that may
reduce the DNA purity and inhibit downstream DNA applications.
11. Avoiding the pellet, transfer the supernatant to a clean 2 ml Collection Tube (provided).
12. Add 200 µl of Solution PW2 and vortex briefly to mix. Incubate at 4°C for 5 minutes.
What’s happening: Solution PW2 is another part of the patented Inhibitor Removal Technology®(IRT) and
is a second reagent to remove additional non-DNA organic and inorganic material including humic acid, cell
debris, and proteins. It is important to remove contaminating organic and inorganic matter that may reduce
DNA purity and inhibit downstream DNA applications.
13. Centrifuge the tubes at 13,000 x g for 1 minute.
14. Avoiding the pellet, transfer the supernatant to a clean 2 ml Collection Tube (provided).
What’s happening: The pellet at this point contains additional non-DNA organic and inorganic material. For
best DNA yields and quality, avoid transferring any of the pellet.
15. Add 650 µl of Solution PW3 and vortex briefly to mix.
Note: Check Solution PW3 for precipitation prior to use. Warm if necessary. Solution
PW3 can be used while still warm.
What’s happening: Solution PW3 is a high concentration salt solution. Since DNA binds tightly to silica at
high salt concentrations this will adjust the DNA solution salt concentration to allow binding of the DNA, but
not non-DNA organic and inorganic material that may still be present at low levels, to the spin filter.
16. Load 650 µl of supernatant onto a Spin Filter and centrifuge at 13,000 x g for 1 minute. Discard
the flow through and repeat until all the supernatant has been loaded onto the Spin Filter.
Note: A total of two loads for each sample processed are required.

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What’s happening: The DNA is selectively bound to the silica membrane in the Spin Filter basket and the
flow through containing non-DNA components is discarded.
17. Place the Spin Filter basket into a clean 2 ml Collection Tube (provided).
What’s happening: Due to the high concentration of salt in solution PW3, it is important to place the Spin
Filter basket into a clean 2 ml Collection Tube to aid in the subsequent wash steps and improve the DNA
purity and yield.
18. Shake to mix Solution PW4 before use. Add 650 µl of Solution PW4 and centrifuge at 13,000 x g
for 1 minute.
What’s happening: Solution PW4 is an alcohol based wash solution used to further clean the DNA that is
bound to the silica filter membrane in the Spin Filter. This wash solution removes residual salt and other
contaminants while allowing the DNA to stay bound to the silica membrane.
19. Discard the flow through and add 650 µl of Solution PW5 and centrifuge at 13,000 x g for 1
minute.
What’s happening: Solution PW5 ensures complete removal of Solution PW4 which will result in higher
DNA purity and yield.
20. Discard the flow through and centrifuge again at 13,000 x g for 2 minutes to remove residual
wash.
What’s happening: The second spin removes residual Solution PW5. It is critical to remove all traces of
wash solution because the ethanol in Solution PW5 can interfere with many downstream DNA applications
such as PCR, restriction digests, and gel electrophoresis.
21. Place the Spin Filter basket into a clean 2 ml Collection Tube (provided).
22. Add 100 µl of Solution PW6 to the center of the white filter membrane.
What’s happening: Placing Solution PW6 (sterile elution buffer) in the center of the small white membrane
will make sure the entire membrane is wetted. This will result in a more efficient and complete release of
the DNA from the silica Spin Filter membrane. As Solution PW6 passes through the silica membrane, the
DNA that was bound in the presence of high salt is selectively released by Solution PW6 (10 mM Tris)
which lacks salt.
Alternatively, sterile DNA-Free PCR Grade Water may be used for DNA elution from the silica Spin Filter
membrane at this step. Solution PW6 contains no EDTA. If DNA degradation is a concern, sterile TE may
also be used instead of PW6 for elution of DNA from the Spin Filter.
23. Centrifuge at 13,000 x g for 1 minute.
24. Discard the Spin Filter basket. The DNA is now ready for any downstream application. No
further steps are required.
We recommend storing the DNA frozen (-20°C to -80°C). Solution PW6 contains no EDTA. To
concentrate the DNA, see the Hints and Troubleshooting Guide.
Thank you for choosing the PowerWater®DNA Isolation Kit!

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Vacuum Protocol using the PowerVac™Manifold
Please wear gloves at all times
For each sample lysate, use one Spin Filter column. Keep the Spin Filter in the attached 2 ml Collection
Tube and continue using the Collection Tube as a Spin Filter holder until needed for the Vacuum
Manifold Protocol. Label each Collection Tube top and Spin Filter column to maintain sample identity. If
the Spin Filter becomes clogged during the vacuum procedure, you can switch to the procedure for
purification of the DNA by centrifugation.
You will need to provide 100% ethanol for step 4 of this protocol
1. For each prep, attach one aluminum PowerVac™ Mini Spin Filter Adapter (MO BIO Catalog#
11992-10 or 11992-20) into the Luer-Lok® fitting of one port in the manifold. Gently press a Spin
Filter column into the PowerVac™ Mini Spin Filter Adapter until snugly in place. Ensure that all
unused ports of the vacuum manifold are closed.
Note: Aluminum PowerVac™ Mini Spin Filter Adapters are reusable.
2. Transfer 650 l of prepared sample lysate (from step 15) to the Spin Filter column.
3. Turn on the vacuum source and open the stopcock of the port. Hold the tube in place when opening
the stopcock to keep the spin filter steady. Allow the lysate to pass through the Spin Filter column.
After the lysate has passed through the column completely, load again with the next 650 l of lysate.
Continue until all of the lysate has been loaded onto the Spin Filter column. Close the one-way
Luer-Lok® stopcock of that port.
Note: If Spin Filter Columns are filtering slowly, close the ports to samples that have
completed filtering to increase the pressure to the other columns.
4. Add 800 l of 100% ethanol into the Spin Filter so that it completely fills the column. Open the
stopcock while holding the column steady. Allow the ethanol to pass through the column completely.
Close the stopcock.
5. Shake to mix Solution PW4. Add 650 l of Solution PW4 to each Spin Filter. Open the Luer-Lok®
stopcock and apply a vacuum until Solution PW4 has passed through the Spin Filter completely.
Continue to pull a vacuum for another minute to dry the membrane. Close each port.
6. Add 650 l of Solution PW5 to each Spin Filter. Open the Luer-Lok® stopcock and apply a vacuum
until Solution PW5 has passed through the Spin Filter completely. Continue to pull a vacuum for
another minute to dry the membrane. Close each port.
7. Turn off the vacuum source and open an unused port to vent the manifold. If all 20 ports are in use,
break the vacuum at the source. Make certain that all vacuum pressure is released before performing
the next step. It is important to turn off the vacuum at the source to prevent backflow into the
columns.
8. Remove the Spin Filter column and place in the original labeled 2 ml Collection Tube. Place into
the centrifuge and spin at 13,000 × g for 2 minutes to completely dry the membrane.

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9. Transfer the Spin Filter column to a new 2 ml Collection Tube and add 100 l of Solution PW6 to
the center of the white filter membrane. Alternatively, sterile DNA-Free PCR Grade Water may be
used for elution from the silica Spin Filter membrane at this step (MO BIO Catalog# 17000-10).
10. Centrifuge at room temperature for 1 minute at 13,000 x g.
11. Discard the Spin Filter column. The DNA in the tube is now ready for any downstream application.
No further steps are required.
Thank you for choosing the PowerWater®DNA Isolation Kit.

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Hints and Troubleshooting Guide
Types of Water Samples
A. Clear Water Samples: Water samples may vary from clear to highly turbid. Larger volumes of
clear water can be processed because there is less chance of filter clogging. Potable drinking
water will generally allow for very high volumes depending on the quality and particulate count. In
most cases, 100 ml to 10 liters can be processed. Some users report processing even higher
volumes.
B. Turbid Water Samples: Turbid samples with high levels of suspended solids or sediments will
tend to clog filters with a smaller pore size (0.22 micron). Use of 0.45 micron filters is
recommended for theses types of samples. MO BIO Laboratories offers disposable filter funnels
containing membranes of either 0.22 micron or 0.45 micron pore sizes (See page 3 for ordering
information). Prior to filtering, samples can be stored in a container to allow suspended solids to
settle out. For samples where settling does not occur or is not desired, a method involving
stacking filters with larger pore sizes on top of the filter membrane of the desired pore size is
recommended. A common set-up is to stack a sterile 1 micron filter. This layering will filter out
large debris and allow the smaller micron filter to trap microorganisms. The layered filter system
can be washed with sterile water or sterile phosphate buffer to knock down some of the trapped
microorganisms on the larger pore size filters. Although this is not 100% efficient, it will increase
the overall yield of microbial DNA
Filter Membrane Selection
MO BIO Laboratories offers disposable filter funnels containing filter membranes commonly used for
water research and testing. The 0.22 micron filter membrane consists of polyethersulfone (Pall Supor®),
while the 0.45 micron filter membrane consists of cellulose acetate. Some filter membranes may bind
and concentrate inhibitors. To reduce the likelihood of this occurring, filter membrane types may need to
be evaluated prior to use.
Forgetting to Warm Solution PW1
If PW1 is not warmed prior to use, continue with the protocol. You will still obtain DNA, but the yields
may not be optimal.
Alternate Lysis Method
Heating can aid in lysis of some organisms (fungi and algae) and lead to increased yields. At Step 5,
heat the PowerWater®Bead Tube at 65°C for 10 minutes then continue with the protocol at Step 6.
If a Centrifuge for 15 ml Tubes is not Available for use with the 5 ml Tubes in Step 8
Centrifugation at this step helps to separate the supernatant from the filter membrane so that as much of
the solution as possible is recovered. If a centrifuge is not available, this step can be skipped with some
minor loss of supernatant.
Expected DNA Yields
DNA yields will vary depending on the type of water, sample location, and time of year. Examples of
expected yields are provided as a reference. Due to diversity of water sample types, yields may fall
outside of the examples provided.

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Hints and Troubleshooting Guide cont.
Type of Water Sample
Sample Volume
DNA Yield (ng/μl)
Saltwater Bay
100 ml
40 - 72
Freshwater Lake
100 ml
15 - 25
Lagoon
20-100 ml
3 - 38
Ocean (coastal)
100 ml
3 - 11
Sewage influent
50 ml
95
Treated effluent
50 ml
18
Low A260/230 Ratios are Obtained
A260/230 readings are one measure of DNA purity. For samples with low biomass, which would lead to low
DNA yields (<20 ng/µl), this ratio may fall below 1.5. This ratio is not an indicator of amplification ability
or DNA integrity. Ethanol precipitation with resuspension into a smaller volume to concentrate the DNA
may help to improve the A260/230 ratio.
DNA Floats Out of Well When Loaded on a Gel
Residual PW5 Wash Buffer may be in the final sample. To ensure complete drying of the membrane
after PW5, centrifuge the spin filter in a clean 2 ml Collection Tube for an additional minute.
Ethanol precipitation is the best way to remove residual Solution PW5. (See “Concentrating the
DNA” below.)
If you live in a humid climate, you may experience increased difficulty with drying of the
membrane in the centrifuge. Increase the centrifugation time at step 20 by another minute or until
no visible moisture remains on the membrane.
Concentrating the DNA (no new tubes are required for this process)
Your final volume will be 100 μl. If this is too dilute for your purposes, add 5 μl of 3M Sodium Acetate and
mix. Then add 2 volumes of 100% cold ethanol. Mix, and incubate at -70°C for 15 minutes or -20oC for 2
hours to overnight. Centrifuge at 10,000 x g for 10-15 minutes at 4°C. Decant all liquid. Briefly dry
residual ethanol in a speed vac or ambient air. Avoid over-drying the pellet or resuspension may be
difficult. Resuspend precipitated DNA in desired volume of 10 mM Tris (Solution PW6).
Storing DNA
DNA is eluted in 10 mM Tris (Solution PW6) and should be used immediately or stored at -20°C or -80°C
to avoid degradation. DNA can be eluted in TE but the EDTA may inhibit reactions such as PCR and
automated sequencing.
Cleaning of the PowerVac™ Mini Spin Filter Adapters
It is recommended to rinse the PowerVac™ Mini Spin Filter Adapters promptly after use to avoid salt
build up. To clean the PowerVac™ Mini Spin Filter Adapters, rinse each adapter with DI water followed
by 70% ethanol and flush into the manifold base. Alternatively, remove the adapters and wash in
laboratory detergent and DI water. PowerVac™ Mini Spin Filter Adapters may be autoclaved.
Do not use bleach to clean the PowerVac™ Mini Spin Filter Adapters while attached to the
PowerVac™ Manifold. Bleach should never be mixed with solutions containing guanidine and
should not be used to clean the PowerVac™ Manifold. For more information on cleaning the
PowerVac™ Manifold, please refer to the PowerVac™ Manifold manual.

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Contact Information
Technical Support:
Phone MO BIO Laboratories, Inc. Toll Free 800-606-6246, or 760-929-9911
Email: [e-mail]
Fax: [phone]
Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA 92010
Ordering Information:
Direct: Phone MO BIO Laboratories, Inc. Toll Free 800-606-6246, or 760-929-9911
Email: [e-mail]
Fax: [phone]
Mail: MO BIO Laboratories, Inc, 2746 Loker Ave West, Carlsbad, CA 92010
For the distributor nearest you, visit our web site at www.mobio.com/distributors

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Other Quality Products Available from MO BIO Laboratories, Inc.
For more product and detailed information go to www.mobio.com/catalog-request to request a catalog.
DNA Purification and Gel Extraction
Catalog No.
Quantity
RNA Isolation …Continued
Catalog No.
Quantity
PowerClean®DNA Clean-Up Kit
12877-50
50 preps
RNA PowerSoil®DNA Elution
Accessory Kit
12867-25
25 preps
UltraClean®15 DNA Purification Kit
12100-300
300 preps
RNA PowerSoil®Total RNA Isolation
Kit
12866-25
25 preps
UltraClean®PCR Clean-Up Kit
12500-50
12500-100
12500-250
50 preps
100 preps
250 preps
UltraClean®Microbial RNA Isolation
Kit
15800-50
15800-250
50 preps
250 preps
UltraClean®-htp 96 Well PCR Clean-
Up Kit
12596-4
12596-12
4 x 96 preps
12 x 96 preps
UltraClean®Tissue & Cells RNA
Isolation Kit
15000-50
15000-250
50 preps
250 preps
UltraClean®GelSpin®DNA
Extraction Kit
12400-50
12400-100
12400-250
50 preps
100 preps
250 preps
UltraClean®Plant RNA Isolation Kit
13300-20
13300-50
20 preps
50 preps
Plasmid DNA Isolation
Catalog No.
Quantity
Genomic DNA Isolation
Catalog No.
Quantity
UltraClean®6 Minute Mini Plasmid
Prep Kit
12300-50
12300-100
12300-250
50 preps
100 preps
250 preps
PowerLyzer™ PowerSoil®DNA
Isolation Kit
12855-50
12855-100
50 preps
100 preps
UltraClean®Standard Mini Plasmid
Prep Kit
12301-50
12301-100
12301-250
50 preps
100 preps
250 preps
PowerLyzer™ UltraClean®Microbial
DNA Isolation Kit
12255-50
50 preps
UltraClean®-htp 96 Well Plasmid Prep
Kit
12396-4
12396-12
4 x 96 preps
12 x 96 preps
PowerBiofilm™ DNA Isolation Kit
24000-50
50 preps
UltraClean®Midi Plasmid Prep Kit
12700-20
12700-50
20 preps
50 preps
PowerFood™ Microbial DNA Isolation
Kit
21000-50
21000-100
50 preps
100 preps
UltraClean®Maxi Plasmid Prep Kit
12600-10
12600-20
10 preps
20 preps
BiOstic®Bacteremia DNA Isolation
Kit
12240-50
50 preps
UltraClean®Endotoxin-Free Mini
Plasmid Prep Kit
12311-100
12311-250
100 preps
250 preps
BiOstic® FFPE Tissue DNA Isolation
Kit
12250-50
50 preps
UltraClean®Endotoxin-Free Midi
Plasmid Prep Kit
12711-10
10 preps
BiOstic®Paraffin Removal Reagent
12251-50
2 x 25 ml
UltraClean®Endotoxin-Free Maxi
Plasmid Prep Kit
12611-10
10 preps
PowerMax®Soil DNA Isolation Kit
12988-10
10 preps
UltraClean®Endotoxin Removal Kit
12615
1 kit
PowerSoil®DNA Isolation Kit
12888-50
12888-100
50 preps
100 preps
UltraClean®Endotoxin-Free Ethanol
Precipitation Kit
12616
1 kit
PowerSoil®-htp 96 Well Soil DNA
Isolation Kit
12955-4
12955-12
4 x 96 preps
12 x 96 preps
UltraClean®Endotoxin Removal
Reagent
12625-25
25 ml
UltraClean®Soil DNA Isolation Kit
12800-50
12800-100
50 preps
100 preps
Endotoxin-Free Sodium Chloride
12626-15
15 ml
UltraClean®-htp 96 Well Soil DNA
Isolation Kit
12896-4
12896-12
4 x 96 preps
12 x 96 preps
Endotoxin-Free Centrifuge Tubes
12617-100
12618-50
12619-25
100 each/2 ml
tubes
50 each/15 ml
tubes
25 each/50 ml
tubes
UltraClean®Mega Soil DNA Isolation
Kit
12900-10
10 preps
RNA Isolation
Catalog No.
Quantity
PowerClean®DNA Clean-Up Kit
12877-50
50 preps
PowerWater®RNA Isolation Kit
(No Filters)
14700-50-NF
50 preps
UltraClean®Fecal DNA Isolation Kit
12811-50
12811-100
50 preps
100 preps
PowerLyzer™ UltraClean®Tissue &
Cells RNA Isolation Kit
15055-50
50 preps
PowerLyzer™ UltraClean®Plant RNA
Isolation Kit
13355-50
50 preps
PowerMicrobial®Midi DNA Isolation
Kit
12225-25
25 preps
PowerBiofilm™ RNA Isolation Kit
25000-50
50 preps
PowerMicrobial®Maxi DNA Isolation
Kit
12226-25
25 preps
LifeGuard™ Soil Stabilization Solution
12868-100
12868-1000
100 ml
1 L
UltraClean®Microbial DNA Isolation
Kit
12224-50
12224-250
50 preps
250 preps
On-Spin Column DNase I Kit (RNase-
Free)
15100-50
50 preps
UltraClean®-htp 96 Well Microbial
DNA Isolation Kit
10196-4
10196-12
4 x 96 preps
12 x 96 preps
BiOstic®Stabilized Blood RNA
Isolation Kit
12231-50
12231-100
50 preps
100 preps
PowerPlant®DNA Isolation Kit
13200-50
13200-100
50 preps
100 preps
BiOstic®Blood Total RNA Isolation
Kit
12230-50
50 preps
UltraClean®Plant DNA Isolation Kit
13000-50
13000-250
50 preps
250 preps

Technical Information: Toll free 1-800-606-6246, or 1-760-929-9911 Email: technica[email protected] Website: www.mobio.com
17
Other Quality Products Available from MO BIO Laboratories, Inc.
For more product and detailed information go to www.mobio.com/catalog-request to request a catalog.
Genomic DNA Isolation
…Continued
Catalog No.
Quantity
Other Reagents and Lab
Accessories
Catalog No.
Quantity
UltraClean®-htp 96 Well Plant DNA
Isolation Kit
13096-4
13096-12
4 x 96 preps
12 x 96 preps
20 bp DNA Ladder
17020-40
40 µg
UltraClean®Tissue & Cells DNA
Isolation Kit
12334-50
12334-250
50 preps
250 preps
100 bp DNA Ladder
17100-40
40 µg
UltraClean®-htp 96 Well Tissue DNA
Isolation Kit
12996-4
12996-12
4 x 96 preps
12 x 96 preps
1 kb DNA Ladder
17200-100
100 µg
UltraClean®Blood DNA Isolation Kit
(Non-Spin)
12000-100
100 preps
UltraClean®Agarose, Molecular
Biology Grade
15003-50
15003-100
15003-500
15003-1000
50 g
100 g
500 g
1 kg
UltraClean®Blood DNA Isolation Kit
(Processes 1,000 ml of Blood)
12000-1000
1 kit
UltraClean®MS-8 Agarose
15515-50
15515-100
15515-500
50 g
100 g
500 g
UltraClean®Blood DNA Isolation Kit
Plus RNase
(Processes 1,000 ml of Blood)
12002-1000
1 kit
UltraClean®Forensic Agarose
15505-50
15505-100
15505-500
50 g
100 g
500 g
UltraClean®Low Melt Agarose
15005-50
15005-100
15005-500
50 g
100 g
500 g
UltraClean®BloodSpin®DNA
Isolation Kit
12200-50
12200-250
50 preps
250 preps
UltraClean®-htp 96 Well BloodSpin®
DNA Isolation Kit
12296-4
12296-12
4 x 96 preps
12 x 96 preps
UltraClean® Low Melt Sieve Agarose
15004-50
15004-100
15004-500
50 g
100 g
500 g
UltraClean®Forensic DNA Isolation
Kit
14000-10
14000-20
10 isolations
20 isolations
Ethidium Bromide Solution
15006-1
15006-10
1 ml
10 ml
PowerWater®Sterivex DNA Isolation
Kit
14600-50-NF
50 preps
Ethidium Bromide Destaining Tea
Bags
15007-25
25 bags
PowerWater®DNA Isolation Kit
(No Filters)
14900-50-NF
14900-100-NF
50 preps
100 preps
Dye Dots™ Dry Gel Loading Dye
with Bromophenol Blue
15020-10
15020-20
10 plates
20 plates
RapidWater™ DNA Isolation Kit
(No Filters)
14810-50-NF
14810-100-NF
50 preps
100 preps
Bromophenol Blue Gel Loading
Buffer
15008-1
15008-5
1 ml
5 x 1 ml
UltraClean®Water DNA Isolation Kit
(No filters)
14800-10-NF
14800-25-NF
10 preps
25 preps
Bromophenol Blue/Xylene Cyanol
Gel Loading Buffer
15009-1
15009-5
1 ml
5 x 1 ml
Microbiological Culture Media
Catalog No.
Quantity
TB DRY®Powder Growth Media
12105-05
12105-1
12105-5
500 g
1 kg
5 kg
TAE Buffer, 50X (Tris-acetate-EDTA)
15001-500
15001-1000
500 ml
1 liter
LB Broth Powder Growth Media, pH
7
12106-05
12106-1
12106-5
500 g
1 kg
5 kg
TBE Buffer, 10X (Tris-borate-EDTA)
15002-500
15002-1000
500 ml
1 liter
LB Agar Powder Growth Media, pH 7
12107-05
12107-1
12107-5
500 g
1 kg
5 kg
RNase-Free Gloves
1556-XS
1556-S
1556-M
1556-L
bag of 150
bag of 150
bag of 150
bag of 150
LB Broth (Lennox) Powder Growth
Media, pH 7
12108-05
12108-1
12108-5
500 g
1 kg
5 kg
UltraClean®Lab Cleaner
12095-250
12095-500
12095-1000
250 ml
squeeze bottle
500 ml spray
bottle
1 liter bottle
LB Agar (Lennox) Powder Growth
Media, pH 7
12109-05
12109-1
12109-5
500 g
1 kg
5 kg
KAPA PROBE FAST qPCR Kits
51220-100
51220-500
51220-1000
100 reactions
500 reactions
1000 reactions
Soybean-Casein Digest Medium
(TSB), USP
12114-05
12114-1
12114-5
500 g
1 kg
5 kg
Soybean-Casein Digest Agar
Medium (TSA), USP
12115-05
12115-1
12115-5
500 g
1 kg
5 kg
KAPA SYBR® FAST Universal 2X
qPCR Master Mix
51230-100
51230-500
51230-1000
100 reactions
500 reactions
1000 reactions
Yeast Extract
12110-05
12110-1
12110-5
500 g
1 kg
5 kg
KAPA2G Robust HotStart ReadyMix
51240-100
51240-500
100 reactions
500 reactions
Tryptone
12111-05
12111-1
12111-5
500 g
1 kg
5 kg
KAPA HiFi HotStart ReadyMix
51250-100
51250-500
100 reactions
500 reactions
Agar, Bacteriological Grade
12112-05
12112-1
12112-5
500 g
1 kg
5 kg
KAPA2G FAST HotStart DNA
Polymerase with dNTPs
51260-100
51260-250
51260-500
100 reactions
250 reactions
500 reactions

Technical Information: Toll free 1-800-606-6246, or 1-760-929-9911 Email: technica[email protected] Website: www.mobio.com
18
Other Quality Products Available from MO BIO Laboratories, Inc.
For more product and detailed information go to www.mobio.com/catalog-request to request a catalog.
Other Reagents and Lab
Accessories…Continued
Catalog No.
Quantity
Instrumentation and
Accessories…Continued
Catalog No.
Quantity
KAPA2G FAST HotStart ReadyMix
51270-100
51270-500
100 reactions
500 reactions
Garnet Bead Tubes, 0.70 mm
13123-50
50 x 2 ml tubes
KAPA Long Range HotStart DNA
Polymerase with dNTPs
51280-100
51280-250
51280-500
100 reactions
250 reactions
500 reactions
Garnet + ¼ Ceramic 15 ml Bead
Tubes, 0.70 mm
13134-50
50 tubes
Garnet + ¼ Ceramic 50 ml Bead
Tubes, 0.70 mm `
13144-10
13144-50
13144-100
13144-500
10 tubes
50 tubes
100 tubes
500 tubes
KAPA Taq Polymerase ReadyMix
51290-250
250 reactions
Glass 15 ml Bead Tubes, 0.1 mm
13135-50
50 tubes
DNase (RNase-Free)
15600-5
15601-100
5 mg
2500 units
Glass 50 ml Bead Tubes, 0.1 mm
13145-10
13145-50
13145-100
13145-500
10 tubes
50 tubes
100 tubes
500 tubes
Glass 15 ml Bead Tubes, 1.0 mm
13136-50
50 tubes
Proteinase K
1223-100
1222-2
100 mg
2 ml (20
mg/ml)
Ceramic 15 ml Bead Tubes, 1.4 mm
13137-50
50 tubes
Ribonuclease A (25 mg/ml)
1202-1
1202-5
1 ml
5 ml
Ceramic 50 ml Bead Tubes, 1.4 mm
13147-10
13147-50
10 tubes
50 tubes
PCR Water
17000-1
17000-5
17000-10
17000-11
1 ml
5 x 1 ml
10 x 1 ml
10 ml bottle
Metal 50 ml Bead Tubes, 2.38 mm
13149-10
13149-50
10 tubes
50 tubes
Molecular Biology Grade Water
17012-200
17012-5200
200 ml
5 x 200 ml
Ceramic Beads, 1.4 mm
13113-325
325 gm
DEPC Treated Water
17011-200
17011-5200
200 ml
5 x 200 ml
Ceramic Beads, 2.8 mm
13114-325
325 gm
Endotoxin-Free Water
17013-10
17013-50
17013-100
17013-500
10 ml
50 ml
100 ml
500 ml
Glass Beads, 0.5 mm
13116-400
400 gm
Metal Beads, 2.38 mm
13117-500
500 gm
UltraClean® 5N NaOH
UltraClean® 0.1N NaOH
UltraClean® 0.5M EDTA, pH 8.0
UltraClean® TE-4 Buffer
17300-30
17301-30
17310-100
17320-1000
30 ml
30 ml
100 m
1L
Glass Beads, 0.1 mm
13118-400
400 gm
Carbide Beads, 0.25 mm
13121-500
500 gm
UltraClean® TE Buffer
UltraClean® 1X PBS, pH 7.4
UltraClean® 2M Tris, pH 8.0
UltraClean® 0.1M Tris, pH 7.0
17325-1000
17330-500
17370-250
17371-1000
1L
500 ml
250 ml
1L
Garnet Beads, 0.15 mm
13122-500
500 gm
Garnet Beads, 0.70 mm
13123-05
500 gm
Instrumentation and Accessories
Catalog No.
Quantity
PowerMix 15 ml Bead Tubes
13138-50
50 tubes
PowerLyzer™ 24 Bench Top Bead-
Based Homogenizer (110/220V)
13155
1 unit
PowerMix 50 ml Bead Tubes
13148-10
13148-50
10 tubes
50 tubes
PowerLyzer™ Tube Holder
13156
1 unit
PowerLyzer™ Tube Holder Stand
13157
1 unit
2 ml Collection Tubes
1200-100-T
1200-150-T
1200-250-T
100 tubes
150 tubes
250 tubes
PowerVac™ Mini System
11992
1 unit + 20
adapters
PowerVac™ Manifold
11991
1 unit
2 ml Screw Cap Tubes
12800-200-E
200 tubes &
caps
PowerVac™ Mini Spin Filter
Adapters
11992-10
11992-20
10 adapters
20 adapters
15 ml Collection Tubes
12700-T
25 tubes
50 ml Centrifuge Tubes
12600-T
25 tubes
Ceramic Bead Tubes, 1.4 mm
13113-50
50 bead tubes
Ceramic Bead Tubes, 2.8 mm
13114-50
50 bead tubes
Spin Filters (in 1.9 ml tubes)
1200-50-SF
1200-100-SF
1200-250-SF
50 filters
100 filters
250 filters
Glass Bead Tubes, 0.5 mm
13116-50
50 bead tubes
Glass Bead Tubes, 0.1 mm
13118-50
50 bead tubes
Endotoxin-Free Centrifuge Tubes
12617-100
12618-50
12619-25
100 each/2 ml
tubes
50 each/15 ml
tubes
25 each/50 ml
tubes
Metal Bead Tubes, 2.38 mm
13117-50
50 bead tubes
2.0 ml Tough Tubes with Cap
13119-500
13119-1000
500
1000
Carbide Bead Tubes, 0.25 mm
13121-50
50 x 0.5 ml
tubes
Garnet Bead Tubes, 0.15 mm
13122-50
50 x 0.5 ml
tubes
15 ml Midi Spin Filters
12700-SF
25 spin filters

Technical Information: Toll free 1-800-606-6246, or 1-760-929-9911 Email: technica[email protected] Website: www.mobio.com
19
Other Quality Products Available from MO BIO Laboratories, Inc.
For more product and detailed information go to www.mobio.com/catalog-request to request a catalog.
Instrumentation and
Accessories… Continued
Catalog No.
Quantity
Vortex-Genie®2 Vortex (120V)
13111-V
1 unit
Vortex-Genie®2 Vortex (220V)
13111-V-220
1 unit
Vortex Adapter, holds 12 (1.5-2.0 ml)
tubes
13000-V1
1 unit
Vortex Adapter, holds 6 (5 ml) tubes
13000-V1-5
1 unit
Vortex Adapter, holds 4 (15 ml) tubes
13000-V1-15
1 unit
Vortex Adapter, holds 2 (50 ml) tubes
13000-V1-50
1 unit
Vortex Adapter, holds 24 (1.5-2.0 ml)
tubes
13000-V1-24
1 unit
Anti-Static Funnels, Micro
23301-96
Pack of 96
Anti-Static Funnels, Small
23302-50
Pack of 50
Anti-Static Funnels, Medium
23303-50
Pack of 50
Anti-Static Funnels, Large
23304-20
Pack of 20
Mini Horizontal Gel System
16001
1 each
Mini Horizontal Gel Caster, 3 place
16003
1 each
Mini Horizontal Gel Tray
16004
1 each
Polycarbonate Single-sided Comb
16005
16006
16007
16008
1 mm x 3 well
1 mm x 8 well
1 mm x 10 well
1 mm x 12 well
Polycarbonate Dual-sided Comb
16013
16014
16015
16016
1 mm x 8
well/16 well
1 mm x 10
well/14 well
2 mm x 8
well/16 well
2 mm x 10
well/14 well
Teflon Single-sided Comb
16009
16010
16011
16012
1 mm x 3 well
1 mm x 8 well
1 mm x 10 well
1 mm x 12 well
Teflon Dual-sided Comb
16017
16018
16019
16020
1 mm x 8
well/16 well
1 mm x 10
well/14 well
2 mm x 8
well/16 well
2 mm x 10
well/14 well
Power Supply w/Timer, (120V)
16023
1 unit
96 Well Plate Shaker (120V)
11996
1 unit
96 Well Plate Shaker (220V)
11996-220
1 unit
Plate Adapter Set
11999
1 set
Vacuum Pump (120V)
11998
1 unit
Vacuum Pump (220V)
11998-220
1 unit