Hermle Z 287 A User manual

© Hermle Labortechnik GmbH Z287A_V1.19_eng
Operating Manual for
Universal Centrifuge
Z 287 A

CONTENTS
© Hermle Labortechnik GmbH Z287A_V1.19_eng I
1. PRODUCT DESCRIPTION ..................................................................................................1
1.1 Safety Instructions ............................................................................................................................................ 1
1.2 Indended Purpose ............................................................................................................................................. 1
1.3 Brief Description................................................................................................................................................ 1
1.4 Delivery Package ............................................................................................................................................... 1
1.5 Installation of the Centrifuge ............................................................................................................................ 2
1.5.1 Unpacking the Centrifuge................................................................................................................................ 2
1.5.2 Space Requirements....................................................................................................................................... 2
1.5.3 Installation ....................................................................................................................................................... 2
1.6 Signs- and Indication of the Centrifuge........................................................................................................... 3
1.6.1 Product Nameplate (Example) ........................................................................................................................ 3
1.6.2 Warning and Information Signs ....................................................................................................................... 4
1.7 Operating and Display Elements...................................................................................................................... 5
1.7.1 LED light.......................................................................................................................................................... 5
1.7.2 LCD-Display .................................................................................................................................................... 6
1.7.3 Danger, Precautions and Warranty ................................................................................................................. 8
1.7.4 Following Rules Must Strictly be Adhered To:................................................................................................. 8
1.7.5 Warranty.......................................................................................................................................................... 9
1.8 Basic Adjustment .............................................................................................................................................. 9
1.8.1 Access to the Mode: “Standard settings“......................................................................................................... 9
1.8.2 Signal Turn On / Off ..................................................................................................................................... 10
1.8.3 Keyboard Sound Turn On / Off...................................................................................................................... 10
1.8.4 Sleep Mode On / Off...................................................................................................................................... 11
1.8.5 Retrieving Operation Data............................................................................................................................. 11
2. OPERATING......................................................................................................................12
2.1 Mounting and Loading the Rotor ................................................................................................................... 12
2.1.1 Installation of Rotors...................................................................................................................................... 12
2.1.2 Loading the Angle Rotor................................................................................................................................ 14
2.1.3 Loading and Overloading of Rotors............................................................................................................... 14
2.1.4 Rotor Identification ........................................................................................................................................ 14
2.1.5 Removing the Rotor ...................................................................................................................................... 14
2.2 Power Switch and Fuse................................................................................................................................... 15
2.3 Lid ..................................................................................................................................................................... 15
2.3.1 Lid Release ................................................................................................................................................... 15
2.3.2 Lid Lock......................................................................................................................................................... 16
2.4 Pre-Selection.................................................................................................................................................... 16
2.4.1 Pre-Selection of Speed and RCF-Value........................................................................................................ 16
2.4.2 Pre-Selection of Running Time...................................................................................................................... 17
2.4.3 Pre-Selection of Brake Intensity and Acceleration......................................................................................... 17
2.5 Radius Correction............................................................................................................................................ 18
2.6 Program............................................................................................................................................................ 18
2.6.1 Storage of Programs ..................................................................................................................................... 18
2.6.2 Recall of Stored Programs ............................................................................................................................ 19

CONTENTS
II Z287A_V1.19_eng © Hermle Labortechnik GmbH
2.6.3 Leaving Program Mode................................................................................................................................. 20
2.7 Starting and Stopping the Centrifuge............................................................................................................ 20
2.7.1 Starting the Centrifuge................................................................................................................................... 20
2.7.2 The “STOP” KEY........................................................................................................................................... 21
2.8 Imbalance Detection........................................................................................................................................ 21
3. MAINTENANCE.................................................................................................................22
3.1 Maintenance and Cleaning.............................................................................................................................. 22
3.1.1 General.......................................................................................................................................................... 22
3.1.2 Cleaning and Disinfection of the Unit ............................................................................................................ 23
3.1.3 Cleaning and Disinfection of the Rotor.......................................................................................................... 23
3.1.4 Disinfection of PP-Rotors .............................................................................................................................. 23
3.1.5 Disinfection of Aluminum Rotors ................................................................................................................... 24
3.1.6 Glass Breakage............................................................................................................................................. 24
3.2 Lifetime of Rotors and Accessories............................................................................................................... 24
4. TROUBLE SHOOTING ......................................................................................................25
4.1 Error Message: Problem / Solution................................................................................................................ 25
4.2 Survey of Possible Error Messages and Solutions ...................................................................................... 25
4.2.1 Lid Release during Power Failure (Emergency Lid Release)........................................................................ 25
4.2.2 Description of the Error Message System..................................................................................................... 26
5. RECEIPT OF CENTRIFUGES TO REPAIR .......................................................................27
6. TRANSPORT, STORAGE AND DISPOSAL......................................................................28
6.1 Transport.......................................................................................................................................................... 28
6.2 Storage ............................................................................................................................................................. 28
6.3 Disposal............................................................................................................................................................ 28
6.4 RoHS II Compliance......................................................................................................................................... 28
7. APPENDIX.................................................................................................................... XXIX
EG –Conformity Declaration ................................................................................................................................... II
Table 1: Technical Data ......................................................................................................................................... III
Table 2: Permissible Net Weight.............................................................................................................................IV
Table 3: Max. Speed and RCF-Values for Permissible Rotors ...............................................................................IV
Table 4: Acceleration and Deceleration Times.........................................................................................................V
Table 5: Error Messages..........................................................................................................................................V
Table 6: Radius Correction .....................................................................................................................................VI
Table 6: Radius Correction ....................................................................................................................................VII
Table 7: Symbols- / Abbreviations..........................................................................................................................VII
Redemption Form: Decontamination Certificate .....................................................................................................IX

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z287A_V1.19_eng 1
1. PRODUCT DESCRIPTION
1.1 Safety Instructions
This symbol indicates safety instructions and points of potential dangerous situations. Before using
the centrifuge for the first time, please read the operating manual.
Failure to follow these instructions can result in personal injury and/or property damage .
Intended use includes: the observation of all instructions, in the instruction manual, and
administering inspection and maintenance.
1.2 Indended Purpose
This Hermle centrifuge was designed only for the separation of materials or mixtures with different
densities, specifically for the preparation and processing of samples, from the human body, in
context of an in-vitro-diagnostic use, to allow the use of in-vitro-diagnostic in accordance to its'
intended purpose. The designated device and its' accessories listed, in the technical documentation,
are in compliance with Directive 98/79/EC for In-Vitro-Diagnostic Medical Devices.
Hermle Centrifuges are intended exclusively for indoor use and for the use of qualified personnel.
Only Hermle original rotors and accessories should be used. Any other use or intended use is strictly
prohibited. For any resulting damage, the company, Hermle Labortechnik, is not liable.
1.3 Brief Description
The unit type Z 287 A is a non-refrigerated universal centrifuge, which we offer in two voltage
variations 230V or 120V.
The centrifuge can be used with swing-out rotors and angle rotors.
All parameters are accessible via buttons and selected with the control field. All pre-selected and
current values will be shown permanently on the LCD-display.
The centrifuge is powered by a maintenance-free induction motor.
Detailed technical data are in Table 1: Technical Data, (see APPENDIX P.III).
1.4 Delivery Package
•1 Centrifuge Z 287 A
•1 Operating Manual Z 287 A
•1 Rotor key
•1 Power Cord
Rotor(s) / Accessories will be packaged separately.

PRODUCT DESCRIPTION
2 Z287A_V1.19_eng © Hermle Labortechnik GmbH
1.5 Installation of the Centrifuge
1.5.1 Unpacking the Centrifuge
Model Z 287 A is supplied in a carton.
Remove the strap retainer, open the carton and remove the centrifuge. The instruction manual must
be kept with the centrifuge at all times!
1.5.2 Space Requirements
The centrifuge should be installed on an even, vibration free and solid surface, if possible on a
laboratory cabinet / table or some other solid vibration free surface.
During centrifugation, the centrifuge must be placed in a way, that there is a minimum space of
30 cm / 12 in on each side of the unit, according to EN 61010-2-020 standards.
Do not place the centrifuge next to a window or a heater where it could be disposed to excessive
heat, as the performance of the unit is based on an ambient temperature of 23°C/73.4°F.
1.5.3 Installation
Follow these steps:
•Check whether the power supply corresponds with the one specified on the manufacturer's
rating label, mounted on the rear panel.
•The line voltage circuit breaker is max. 10 A (Type K), slowly release for commonly used
instruments.
•In case of emergency, there must be an emergency switch off installed outside of the room, in
order to disconnect the power supply from the unit.
•Connect the centrifuge with the mains.
•(The socket for the power cord must be easy to reach, respectively easy to disconnect).
•Switch on, by using the mains power switch (2.2).
•Open the lid, by using the button LID.
•Remove the transport securing device of the motor

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z287A_V1.19_eng 3
1.6 Signs- and Indication of the Centrifuge
1.6.1 Product Nameplate (Example)
Company Address:
Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen
TYPE:
Type Designation of the Product
REF:
Order No. of the Product
SN:
Serial No. of the Product
Manufacturer
Date of Manufacture
MAX. Drehzahl:
Max. Speed Allowed of the Unit
KIN. EN.:
Max. Kinetic Energy with Corresponding Rotor
U/I/f:
Allowable Voltage / Max. Current / Frequency
P:
Electrical Input Power
Operating Manual Indication
Labeling, Standards and Guidelines
Instructions for Disposal (see Chapter 6.3, Page 28)
RoHS-Compliance Label (see Chapter 6.4, P28)

PRODUCT DESCRIPTION
4 Z287A_V1.19_eng © Hermle Labortechnik GmbH
1.6.2 Warning and Information Signs
Four carriers must be used at all times on four places swing out
rotors or damage will occur to the centrifuge. Such damage will not
be covered under the product warranty
Attention! Check the fastening of the rotor nut before each run
Take off mains plug before opening the housing or the emergency
release
Power Input
Fuse 2,5 AT
Direction of Rotation –clockwise rotation for the rotor drive
Biohazard Warning

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z287A_V1.19_eng 5
1.7 Operating and Display Elements
1control field Run Parameters
3LCD Control Panel Display
4rpm/rcf Speed/ g-force
5 accel/decel Acceleration / Deceleration Intensity
6time Centrifugation Time
8quick Short Running
9start Start Centrifugation
10 lid/stop Lid Release / Stop Centrifugation
11 prog Retrieving Stored Programs
12 store Program Store
13 LED light Shows the status of the centrifuge
1.7.1 LED light
The LED light indicates the current operation state of the centrifuge. The following table shows all
operating states.
Color of LED light
Operating state
Green
Run complete, lid is closed
Yellow
Lid opened, no sleep mode
Red flashing fast
Error message
Red flashing slow
Centrifuge runs
Yellow flashing slow
Sleep mode
Red –Green flashing slow
Standard settings menu

PRODUCT DESCRIPTION
6 Z287A_V1.19_eng © Hermle Labortechnik GmbH
1.7.2 LCD-Display
The following picture shows the individual elements of the LCD-Display.
Display Fields:
A-1 Display Field –"rpm/rcf"
A-2 Display Field –"acc/dec"
A-3 Display Field–"time"

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z287A_V1.19_eng 7
Messages/Logo of the Display Field:
M1 „close“ M8 „decel“
M2 „open“ M9 „radius“
M3 „rotor“ M10 „programm“
M4 Rotor-Nr. M11 „error“
M5 „rpm“ M12 „service”
M6 „rcf“ M13 h m s
M7 „accel“
Indication:
After switching on the centrifuge, the display "rpm/rcf"(A-1) shows the current software version and
after the model type (example with 207M).
Figure 1

PRODUCT DESCRIPTION
8 Z287A_V1.19_eng © Hermle Labortechnik GmbH
1.7.3 Danger, Precautions and Warranty
This device may only be operated by a trained professional. Carefully, read the
operating manual and be familiar with the functions of the device.
To protect people and the environment, the following precautions must be taken:
•During centrifugation, the presence of people and the arrangement of hazardous materials is
strictly prohibited, within 30 cm/12 in around the centrifuge, according to the regulations of
EN 61010-2-020.
•The HERMLE Z 287 A is non "explosion-proof" and must not be operated in explosion-
endangered areas or locations. Centrifugation of flammable, explosive, radioactive, or such
substances, which chemically react with high energy, is strictly prohibited. If used in such
environment, this is at the users own expense.
•Never spin toxic or pathogenic material without adequate safety precautions, i.e.
centrifugation of tubes with or without defective hermetic sealing, is strictly prohibited. The
user is obliged to perform appropriate disinfection procedures, in case dangerous substances
have contaminated the centrifuge and/or its' accessories. When centrifuging infectious
substances, always pay attention to the General Laboratory Precautions. If necessary,
contact your safety officer!
•It is prohibited to run the centrifuge, with rotors not manufactured for this unit.
•Under no circumstances open the lid of the centrifuge, while the rotor is still running or
rotating with a speed of > 2m/s.
1.7.4 Following Rules Must Strictly be Adhered To:
•Do not operate the centrifuge if not installed correctly.
•Do not operate the centrifuge when dismounted (e.g. without housing).
•Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered
with, by unauthorized personnel.
•Do not use accessories such as rotors and adapters, that are not approved by HERMLE
Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or
plastic.
•Do not spin extremely corrosive substances, as they may cause material damages and impair
mechanical resistance.
•Do not operate the centrifuge with rotors or adapters, which show any signs of corrosion or
mechanical damage.
The manufacturer is responsible for safety and reliability, of the centrifuge, only if:
•The unit is operated in accordance to this instruction manual.
•Modifications, repairs or other adjustments are performed by HERMLE-authorized personnel
and the electrical installation of the related location corresponds to the IEC-regulations.

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z287A_V1.19_eng 9
1.7.5 Warranty
The centrifuge has been subjected to thorough testing and quality control. In the unlikely case of any
manufacturing faults occurring, the centrifuge and rotors are covered by warranty, for a period of two
years, from date of delivery. This warranty becomes invalid in any case of mishandling, damage
and/or negligence and further in any case of usage of inappropriate spare parts and / or accessories
or unauthorized modification of the unit.
Technical modification rights are reserved, by the manufacturer, in regards to
technical improvement!
1.8 Basic Adjustment
1.8.1 Access to the Mode: “Standard settings“
While starting this model, the following basic settings can be performed:
-Signal Turn On / Off
-Keybord Sound On / Off
-Sleep Mode On / Off
The following operating data can be retrieved in this menu:
-Number of Starts
-Operating Hours of Centrifuge
-Operating Hours of Motor
-Software Version
-Error List
-Function of the Imbalance Switch
-Operation of Keyboard
-Hardware Version
-Intermediate Circuit Voltage in Volt
-Display Tests
Open the centrifuge lid and shut off the main switch. Now switch on again the main switch. For
approximately 3 seconds the software version and the model type will be shown in the display (see
Figure 1). Press during this time the keys “time“ (6) and “lid/stop“ (10) simultaneously. As a result, a
display test is administered for approx. 3 seconds. All possible indications will appear at the same
time (see Figure 2). The LED light is flashing alternately with green and red color.
Figure 2

PRODUCT DESCRIPTION
10 Z287A_V1.19_eng © Hermle Labortechnik GmbH
ATTENTION:
-The normal program mode can be changed back again by switching off the centrifuge,
for a short period!
-All changed settings must be confirmed by the key “store” or “start” (9). A confirmation
screen will appear with the word, “store”, in the display “rpm/rcf” (A-1) - Only then the
pre-selections are valid!
1.8.2 Signal Turn On / Off
Proceed as illustrated, under point 1.8.1, to enter this program mode, press the key; "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter, "L" with the
control field (1). As a result, appearing in the display "rpm/rcf" (4), are the words, "On Sound". By
pressing the key, "rpm/rcf" (4), the word "On" flashes, and the sound can be switched off with the
control field (1), (see Figure 3).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
Figure 3
1.8.3 Keyboard Sound Turn On / Off
Proceed as illustrated, under point 1.8.2, to enter this program mode, press the key, "accel/decel"
(5). In the display, "accel/decel" (A-2) flashes the word, "service". Select the letter "b" with the control
field (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word "ON/BEEP". After pressing
the key, "rpm/rcf" (4), turn the keyboard sound (On) or (Off), with the rotorary field (1), (see Figure
4).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
2
3

PRODUCT DESCRIPTION
© Hermle Labortechnik GmbH Z287A_V1.19_eng 11
Figure 4
1.8.4 Sleep Mode On / Off
After not using the centrifuge for 5 minutes with opened lid, the display automatically switches off.
The display is switching off and the LED light is flashing slowly with yellow color. By pressing any
bottom, it switches on again. Proceed as illustrated, under point 1.8.2, to enter this program mode,
press the key, "accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service". Select
the letter "I" with the control field (1). As a result, appearing in the display, "rpm/rcf" (A-1), the word
"SLEEP". After pressing the key, "rpm/rcf" (4), turn the sleep mode function (On) or (Off), with the
rotorary field (1).
After the settings have been stored by user, the normal program mode can be changed back again
by switching off the centrifuge, for a short period.
1.8.5 Retrieving Operation Data
In the function, "Basic Adjustments" the operating data, of the centrifuge, can be retrieved.
Please proceed as described, under point 1.8.1, to enter this program mode.
Press the key "accel/decel" (5). In the display, "accel/decel" (A-2) flashes the word, "service".
With the control field (1) the following information can be retrieved:
A = Previous Starts of the Centrifuge
H = Previous Operating Hours
h = Running Time of the Motor
S = Software Version
E = List of Previous Error Messages
F = Function of the Imbalance Sensor
P = Operating of Keyboard
d = Hardware-Version
U = Intermediate Circuit Voltage in Volt
The list of the last 99 error messages can be looked over by pressing the key “rpm/rcf” (4) and leaf
through it with the control field (1). The respective error codes appear in the display “rpm/rc”(A-1)
(see Figure 5). The first two numbers indicate the appeared errors ongoing from 00 to 99, the last
two numbers indicate the error code. Please refer to, Table 5: Error Messages, (see APPENDIX V).
Switch off the centrifuge, to return to the normal program mode.

OPERATING
12 Z287A_V1.19_eng © Hermle Labortechnik GmbH
Figure 5
2. OPERATING
2.1 Mounting and Loading the Rotor
2.1.1 Installation of Rotors
Clean the drive shaft and the rotor shaft with a clean, grease-free piece of cloth. Place the rotor onto
the drive shaft, (see Figure 6). Please be sure that the rotor is fully installed onto the motor shaft.
Figure 6
Figure 7
Hold the rotor with one hand and secure the rotor to the shaft, by turning the rotor nut clockwise (see
Figure 7)
Some rotors have to be locked with the rotor key. Therefore, hold the rotor with one hand and
secure it by turning the fixing nut clockwise (see Figure 8). The rotor key is supplied with the
respective rotor.

OPERATING
© Hermle Labortechnik GmbH Z287A_V1.19_eng 13
Figure 8
ATTENTION:
For safety, always ensure that the rotor nut is tightened before each run!
Do not operate the centrifuge with rotors or adapters that show any signs of corrosion or
mechanical damage.
Do not operate with extremely corrosive substances, which could damage the rotor and the
centrifuge.

OPERATING
14 Z287A_V1.19_eng © Hermle Labortechnik GmbH
2.1.2 Loading the Angle Rotor
Rotors must be loaded symmetrically and with equal weight, (see Figure 9 and 10). The adapter may
only be loaded with the appropriate vessels. The weight differences between the filled vessels
should be kept as low as possible. Therefore, we recommend to weigh with a balance. This reduces
the wear of drive and the acoustic operating noise.
Each rotor indicates what the maximum capacity is per hole. (It is allowed to operate e.g. a 24-place-
rotor with 2 or 4 loaded tubes only, but the loaded borings must be opposite of each other).
Figure 9: WRONG
Figure 10: RIGHT
2.1.3 Loading and Overloading of Rotors
All approved rotors are listed with the maximum speed and maximum filling weight in, Table 2:
Permissible Net Weight, (see APPENDIX P. IV).
The maximum load permitted for the rotor is determined by the manufacturer, as well as the
maximum speed allowed for this rotor (see label on rotor), must not be exceeded. The liquid the
rotors are loaded with, should have a max. homogeneous density of 1.2 g/ml or less when the rotor
is running at maximum speed.
In order to spin liquids with a higher density, the speed has to be reduced, according to the following
formula:
Reduced speed nred =√1,2
ℎ𝑖𝑔ℎ𝑒𝑟 𝑑𝑒𝑛𝑠𝑖𝑡𝑦 x max. speed (nmax) of the rotor
Example:
nred = √ 1,2
1,7 x 4.000 = 3.360 rpm
If In case of any questions, please contact the manufacturer!
2.1.4 Rotor Identification
The model Z 287 A comes with an automatic rotor identification system. Place the rotor on the motor
shaft as described in 2.1.1 and close the centrifuge lid. The centrifuge immediately recognizes the
rotor and shows its item number on the display.
2.1.5 Removing the Rotor
Untighten the rotor nut completely, counter-clockwise. Lift the rotor vertically out of the centrifuge
(see Figure 7 and Figure 8).

OPERATING
© Hermle Labortechnik GmbH Z287A_V1.19_eng 15
2.2 Power Switch and Fuse
The power switch and the fuse are located on the back side of the centrifuge
Figure 11
In case of a faulty fuse, first disconnect the power cord from the mains supply.
Then open the fuse drawer and replace the fuse with 2.5 AT.
If the fuse triggers again after mains connection, please search the cause of the error and eliminate
it.
ATTENTION:
After turning on the power switch, open and close the centrifuge lid first, before starting the
centrifuge.
2.3 Lid
2.3.1 Lid Release
After the run, properly close the lid of the centrifuge, appearing in the display, "rpm/rcf"(A-1) with the
word, "close" (M1). At the same time the actual rotor type “221.59”is shown in the display. By
pressing the key, "lid/stop" (10), the lid of centrifuge can be released. As soon as the lid is
completely released, the word, "open" (M2) appears. The lid of the centrifuge is now able to be
opened.
For all number marked text, please refer to Figure 12.
Figure 12
Fuse drawer with fuse 2.5 AT

OPERATING
16 Z287A_V1.19_eng © Hermle Labortechnik GmbH
2.3.2 Lid Lock
The lid must only be closed slightly. After closing the lid, the word "open" (M2) will no longer be
displayed. As a sign that the centrifuge is ready for starting, appearing in the display, "rpm/rcf"(A-1),
the word "close" (M1). Simultaneously, the word "rotor" (M3) appears, as well as the code number of
the rotor, which is in the centrifuge, along with all rotor specific data, for example: max. speed,
acceleration etc., are available.
Please refer to Figure 12.
ATTENTION:
Before closing the lid, please check if the rotor is tightened.
2.4 Pre-Selection
2.4.1 Pre-Selection of Speed and RCF-Value
By Selecting the key, "rpm/rcf" (4), pre-selection is activated. By pressing the key once, the word
"rpm" (M5) flashes. By pressing the key again, the pre-selection of the centrifugal forces can be
chosen. The flashing word, "rcf" (M6), will appear.
The desired values can be selected, with the control field (1). In the display (A-1), the regulated
value is shown permanently: before, during and after the run.
This pre-selected value will be stored, as long as a new pre-selection is made.
For all number marked text, please refer to Figure 13.
Figure 13
As long as the centrifuge lidis opened, the speed is adjustable between 200 rpm and the maximum
revolution of the centrifuge.
If the centrifuge lid is closed, the speed can only be pre-selected up to the maximum permissible
revolution of the selected rotor type. It is the same with the pre-selection of the RCF-Value. The
setting range is between 20 xg and the maximum permissible centrifugal force of the rotor.
See Table 3: Max. Speed and RCF-Values for Permissible Rotors, (see APPENDIX P. IV). All
important values are listed on this table.
ATTENTION:
Please notice the maximum permissible revolutions of the test tubes (Producer Indication)!
See also chapter 3.1.6