MO BIO Laboratories PowerLyzer 12155 User manual

INSTRUCTION MANUAL
PowerLyzer™24 Bench Top Bead-Based
Homogenizer
MO BIO Laboratories, Inc.
2746 Loker Avenue West
Carlsbad, CA 92010
P: [phone]
F: [phone]
www.mobio.com
13155 rev. 070110

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WARRANTY INFORMATION
This instruction manual is a guide for the use of the PowerLyzer™24 Bench Top Bead-Based
Homogenizer and accessories.
Data herein has been verified and validated. It is believed adequate for the intended use of the
instrument. If the instrument or procedures are used for purposes other than specified herein,
confirmation of the validity and suitability should be obtained; otherwise MO BIO Laboratories, Inc.
does not guarantee results and assumes no obligation or liability. This publication is not a license to
operate under, or a recommendation to infringe upon, any process patents.
Notes, cautions, and warnings within the text of this manual are used to emphasize important and
critical instructions.
This MO BIO Laboratories, Inc. product is warranted to be free from defects in material and
workmanship for a period of ONE YEAR from the date of delivery. MO BIO Laboratories, Inc. will
repair or replace and return free of charge any part which is returned to its factory within said period,
transportation prepaid by user, and which is found upon inspection to have been defective in materials
or workmanship. This warranty does not include normal wear from use; it does not apply to any
instrument or parts which have been altered by anyone other than an employee of MO BIO
Laboratories, Inc. nor to any instrument which has been damaged through accident, negligence, failure to
follow operating instructions, the use of electric currents or circuits other than those specified on the
plate affixed to the instrument, misuse, or abuse. MO BIO Laboratories, Inc. reserves the right to
change, alter, modify, or improve any of its instruments without any obligation to make corresponding
changes to any instrument previously sold or shipped.
THE FORGOING OBLIGATION IS IN LIEU OF ALL OBLIGATIONS AND LIABILITIES INCLUDING
NEGLIGENCE AND ALL WARRANTIES OF MERCHANTABILITY OR OTHERWISE, EXPRESSED OR
IMPLIED IN FACT OR BY LAW, AND STATE OUR ENTIRE AND EXCLUSIVE LIABILITY AND
BUYERS EXCLUSIVE REMEDY FOR ANY CLAIM OF DAMAGES IN CONNECTION WITH THE SALE
OR FURNISHING OF GOODS OR PARTS, THEIR DESIGN, SUITABILITY FOR USE, INSTALLATION,
OR OPERATION. MO BIO LABORATORIES, INC. WILL IN NO EVENT BE LIABLE FOR ANY
SPECIAL OR CONSEQUENTIAL DAMAGES WHATSOEVER, AND THEIR LIABILITY UNDER NO
CIRCUMSTANCES WILL EXCEED THE CONTRACT PRICE FOR THE GOODS FOR WHICH
LIABILITY IS CLAIMED.

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IMPORTANT SAFEGUARDS
READ ALL INSTRUCTIONS BEFORE USING. SAVE THIS INSTRUCTION MANUAL.
The PowerLyzer™24 Homogenizer has been engineered for maximum functionality as well as safety;
however, basic safety precautions and common sense must always be demonstrated when using any
electrical product. Do not attempt to modify any part of the PowerLyzer™24 Homogenizer. If you
experience problems with or have questions about your PowerLyzer™24 Homogenizer, contact
Technical Support at MO BIO Laboratories, Inc. at 1-800-606-6246 or 760-929-9911.
DANGER
DO NOT allow the machine to be submerged in any liquid.
DO NOT use in any setting other than an indoor laboratory.
DO NOT plug power cord into an incorrect outlet.
WARNING
To reduce the risk of burns, electrocution, fire, or injury:
Use this product only for its intended purpose as described in this manual. Do not use
attachments not recommended by the manufacturer.
Check that the proper power cord is plugged into the correct outlet, and that the voltage
selection switch located on the back of the unit has been selected to the correct voltage for
your country.
DO NOT operate the product if it is damaged in any way.
Keep this product away from heated surfaces.
Biological Risks
Wear gloves when handling samples and strictly follow all of the safety instructions related to bio-
hazardous agents to prevent any risk of contamination. The waste produced by the normal
operation of the instrument must be disposed of in biological waste containers and handled by
specialized companies.

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Table of Contents 
WARRANTY INFORMATION.................................................................................................... 1
SECTION 1. POWERLYZER™24 BENCH TOP BEAD-BASED HOMOGENIZER............... 4
1.1 IMPORTANT INFORMATION.......................................................................................................................... 4
1.2 SPECIFICATIONS..................................................................................................................................................5
1.3 PARTS.......................................................................................................................................................................6
SECTION 2. SET UP AND OPERATION.................................................................................. 8
2.1 UNPACKING THE POWERLYZER™24 HOMOGENIZER.......................................................................8
2.2 TUBE MOTION ...................................................................................................................................................10
2.3 PROGRAMMING.................................................................................................................................................10
2.4 KEYPAD LAYOUT..............................................................................................................................................11
2.5 START UP OF THE EQUIPMENT...................................................................................................................12
2.6 LOADING SAMPLE TUBES ..............................................................................................................................13
2.7 PROGRAM SETTINGS.......................................................................................................................................15
2.8 ADJUSTING PARAMETERS..............................................................................................................................16
2.9 RECOMMENDED SETTINGS ..........................................................................................................................19
2.10 HOMOGENIZER SPEED CONVERSION TABLE ...................................................................................20
SECTION 3. TROUBLESHOOTING........................................................................................ 21
3.1 ERROR MESSAGES ............................................................................................................................................21
3.2 TROUBLESHOOTING ...................................................................................................................................21
SECTION 4. MAINTENANCE .................................................................................................. 22
4.1 REGULAR MAINTENANCE.............................................................................................................................22
4.2 CLEANING AND DECONTAMINATION ..................................................................................................23
SECTION 5. TRANSPORT AND STORAGE .......................................................................... 24
5.1 TRANSPORTING THE POWERLYZER™ 24 HOMOGENIZER............................................................24
5.2 STORAGE..............................................................................................................................................................25
SECTION 6. SUPPORT AND SERVICE................................................................................... 25
6.1 ASSISTANCE.........................................................................................................................................................25
6.2 DECONTAMINATION .....................................................................................................................................25
6.3 DECONTAMINATION CERTIFICATION FORM .....................................................................................26

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SECTION 1. POWERLYZER™24 BENCH TOP BEAD-BASED
HOMOGENIZER
1.1 IMPORTANT INFORMATION
This instruction manual includes all required information regarding unpacking, installation, operation, and
maintenance of the PowerLyzer™24 BenchTop Bead-Based Homogenizer.
Operating this equipment in ways other than those detailed in this instruction manual may impair the
unit.
DO NOT turn the unit upside down: the PowerLyzer™24 Homogenizer must always rest
on all four feet to avoid damaging internal components or breaking the housing.
DO NOT operate the unit when the housing is removed; potentially lethal voltage exists
within the instrument.
DO NOT operate the unit with the safety ground disconnected.
DO NOT overfill the tubes as this may lead to liquid contamination and compromise the
safety of the unit.
CHECK that the proper power cord is plugged into the correct outlet, and that the voltage
selection switch located on the back of the unit has been selected to the correct voltage for
your country.
 
USA: 115V Power Cord European: 230V Power Cord
W
ARNING: Check that the voltage indicated on the rear panel of the unit matches the local power
supply.
RISK OF ELECTRIC SHOCK: Although this equipment is fully insulated and grounded, it is
important for all users to be aware of the potential hazard of using liquids close to a power supply. If
any liquids are spilled, immediately disconnect the instrument from the main power supply (remove
the power cord from the AC power input on the rear panel) and clean the equipment and the
surrounding area. DO NOT reconnect the equipment until it has been fully inspected.

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The PowerLyzer™24 Homogenizer has been designed to lyse and homogenize biological samples
contained in bead beating tubes at variable speeds. It can simultaneously process up to 24 samples in 2
ml tubes at very high speed.
Advantages of the PowerLyzer™24 Homogenizer:
Simultaneously homogenize 24 x 2 ml tubes
Greater sample flexibility for any type of sample processing; broader speed range, number of
cycles, time, dwell/pause
Unique front-loading tube position for ease of use and optimal bead beating interaction
Process hundreds of samples per day
99 custom programmable settings
Disposable tubes ensure no threat of cross-contamination or sample degradation
1.2 SPECIFICATIONS
Depth: 17 in./43.2cm
W
idth: 15.5 in./39.4cm
Height: 13 in./33cm (24 in./61cm tall with lid open)
W
eight: 54 lbs./24kg
Motor Speed: 500-5,000 rpm
Noise Level: <70db
Electrical Requirements: 115 volts, 60Hz or 230 volts, 50Hz
Standards
Approval/Compliance:
CE certified
CAUTION: This is a Class A apparatus. The equipment may cause radio-electric interference in a
residential environment. In this case, it is recommended that the user takes appropriate measures.

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1.3 PARTS
Prior to operation, please remove all parts from the shipping container and inspect for damaged or
missing parts. If any parts are found to be damaged or missing, please contact MO BIO Laboratories, Inc.
at 1-800-606-6246.
The PowerLyzer™24 Homogenizer consists of the following:
Description Quantity
Motor Drive Unit (115V or 230V) 1
Finger Plate 1 Black plastic
Tube Holde
r
1 Blue anodized aluminum
Power Cord 2
115V and 230V
Spare Fuse 1
Instruction Manual 1
Bead tubes for the PowerLyzer™24 Homogenizer are available separately from MO BIO.
Description Cat. no.
Metal Bead Tubes, 2.38 mm 13117-50
Ceramic Bead Tubes, 1.4 mm 13113-50
Ceramic Bead Tubes, 2.8 mm 13114-50
Glass Bead Tubes, 0.1 mm 13118-50
Glass Bead Tubes, 0.5 mm 13116-50
Carbide Bead Tubes, 0.25 mm 13121-50
Garnet Bead Tubes, 0.7 mm 13123-50
Garnet Bead Tubes, 0.15 mm 13122-50
NOTE: Use only accessories and disposables recommended by the manufacturer. Accuracy and
operating lifetime may be affected if alternative products are used. Any damage caused by non-
recommended products are not covered by the manufacturer’s warranty.

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Parts of the PowerLyzer™24 Homogenizer
Finger Plate
Keypad
Door Cove
r
Locking Latch
Main Power
Supply Plug
Hub Lock Switch to lock
Finger Plate and secure
tubes
Tube Holde
r
ON/OFF Switch
Fuses
Air Vent
Cooling Fan
Shipping Screws
115V/230V
Voltage Selection Switch

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SECTION 2. SET UP AND OPERATION
2.1 UNPACKING THE POWERLYZER™ 24 HOMOGENIZER
2.1 UNPACKING THE POWERLYZER™24 HOMOGENIZER
1. Unscrew the two external screws from the back of the unit to release the support plate.
2. Remove the PowerLyzer™24 Homogenizer from the box and place it on a clean, horizontal and
stable surface. WARNING HEAVY ITEM: use caution when lifting to avoid back injury.
W
ARNING: DO NOT connect the unit to the main power supply un
t
il installation is complete
and the correct voltage is selected. DO NOT turn the unit upside down. The PowerLyzer™24
Homogenizer must always rest on its four feet to avoid damaging internal components or breaking
the housing.
CAUTION: DO NOT lift the instrument by holding the door. It must be lifted by holding the
bottom of the unit. Unpack the PowerLyze
r
™
24 Homogenizer with care and inspect it carefully.
NOTE: SAVE the crate, pallet, protective foam, bubble wrap, support plate, and two screws
included with the shipping crate in case a return is necessary. The PowerLyzer™24 Homogenizer
must be shipped in an approved packaging crate to avoid damage which may invalidate the warranty.
Packaging crates may be purchased for a minimal charge. For shipping inquiries, contact MO BIO at
1-800-606-6246.

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3. Check that the voltage selection switch located at the back of the unit matches the voltage
delivered by your electric power supply.
4. Ensure that the air vents and the cooling fan are not covered. Allow at least 6 inches of space
around air vents for proper motor ventilation.
5. Plug the PowerLyzer™24 Homogenizer into the power supply using the proper compatible power
cord supplied (115V or 230V).
In the event of a malfunction or breakdown, grounding provides a path of least resistance for electric
current to reduce the risk of electric shock. This instrument is equipped with an electric cord which is
grounded to the chassis housing. The plug must be plugged in to an outlet that is properly installed and
grounded in accordance with all local codes and ordinances.
W
ARNING: If the voltage supplied to the
equipment is higher than what is indicated on
the voltage selection switch, the electronics
can be irreversibly damaged and the warranty
will be voided.
W
ARNING: DO NOT modify the plug or cords
t
hat are provided. Damaged or worn power
cords should be repaired or replaced immediately by a qualified electrician.

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2.2 TUBE MOTION
Due to the equipment’s design and symmetry, the 24 tubes strictly follow the same motion. This
ensures a consistent level of lysis and homogenization for each sample. The mixture contained in the
bead beating tubes moves throughout the tubes and moves primarily in the vertical axis to allow for
efficient homogenization. Tubes must be evenly balanced on the tube holder.
The following MO BIO bead beating tubes are recommended for use with the PowerLyzer™24
Homogenizer:
Cat. no. Description
13117-50 Metal Bead Tubes, 2.38 mm
13113-50 Ceramic Bead Tubes, 1.4 mm
13114-50 Ceramic Bead Tubes, 2.8 mm
13118-50 Glass Bead Tubes, 0.1 mm
13116-50 Glass Bead Tubes, 0.5 mm
13121-50 Carbide Bead Tubes, 0.25 mm
13123-50 Garnet Bead Tubes, 0.7 mm
13122-50 Garnet Bead Tubes, 0.15 mm
2.3 PROGRAMMING
The PowerLyzer™24 Homogenizer can be programmed for speed, time, number of runs, and the length
of the dwell or pause between programs. The PowerLyzer™24 Homogenizer memory can store up to
99 custom programs, allowing for maximum repeatability and flexibility.
*Dwell minimum varies depending upon the speed and length of cycle.
Paramete
r
Operating range
Speed From 500
t
o 5,000 rpm in increments of 100 rpm
Time From 0:01 to 9:59 in increments of 1 second
Number of Cycles 01 to 10
Dwell/Pause between Cycles Dwell minimum* up to 9:59
Storable Programs 1 to 99

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2.4 KEYPAD LAYOUT
The keypad of the PowerLyzer™24 Homogenizer (see diagram below) consists of an LCD screen, 4
arrow buttons, and a “Start/OK” and “Stop/Pause” button.
The user can adjust four different homogenization settings with this interface. The UP and DOWN
arrow buttons are used to navigate through the menu and the LEFT and RIGHT arrow buttons are
used to adjust parameters.

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2.5 START UP OF THE EQUIPMENT
Turn the PowerLyzer™24 Homogenizer on by pressing the ON/OFF switch located on the rear panel
near the AC power input. When the unit is turned on, a welcome screen is displayed for five seconds
showing the following information:
From this screen, the user can select the display language by pressing and holding to display the
language. The user can then choose from English, Italian, French, German, or Spanish by pressing the 
and arrow keys.
After five seconds, a menu displays the programming mode. Programming mode displays the last
program run before the power was turned off. Example:
“S=” denotes speed of the motor ranging from 500 to 5,000 RPM, in increments of 100 RPM.
“T=” denotes the time interval in minutes:seconds. The variable can range from 0:01 seconds to
9:59 minutes, in increments of 1 second.
“C=” denotes the number of times the cycle (speed x time) is repeated. The variable can range
from 01 to 10, in increments of 1.
“D=” denotes the time interval in seconds of dwell/pause between each cycle. The variable can
range from “Dmin” (Dwell minimum* expressed as min:sec) to 9:59, in increments of 1 second.
*Dwell minimum varies depending upon the speed and length of cycle.
MO BIO
LABORATORIES
POWERLYZER 24
rev XX/XX/XXX
PROG>01 20°C
S=4000 C=02
T=0:10 D=0:10
READY TO START
ChamberTemperature
NumberofCycles
TimeintervalorDwellbetweeneachCycle
SystemStatus
ProgramNumber
SpeedinRPM
CycleTime

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2.6 LOADING SAMPLE TUBES
Follow the steps listed below to load samples into the PowerLyzer™24 Homogenizer.
1. Place bead tubes symmetrically into the tube holder.
If homogenizing less than 24 samples, make sure the tubes
are balanced in the tube holder.
The optional PowerLyzer™Tube Holder Stand (Cat. no. 13157)
simplifies tube loading and storage before and after processing
and is sold separately.
TubeHolderStand
(soldseparately)
2. Place the tube holder onto the carousel.
3. Secure the finger plate in place over the
carousel and screw about 1/8th of a turn past
where the fingers contact the top of the tubes.
Do not over tighten!
Carousel
BlackMotorGasket
CAUTION:Before operating the PowerLyze
r
™
24 Homogenize
r
, ensure that the black motor gasket
is ali
g
ned
p
ro
p
erl
y
on the motor housin
g
. This
g
asket can shift durin
g
shi
p
ment.
TubeHolder
FingerPlate

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4. Move the hub lock switch on the finger
plate to the ‘Lock’ position.
5. Close the door until the locking latch
engages and locks.
Finger Plate
Tube Holde
r
W
ARNING: The carousel can become very hot during operation. Use caution when handling the
tube holder.
HubLock
Switch

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2.7 PROGRAM SETTINGS
After powering on the PowerLyzer™24 Homogenizer, the LCD screen will display the program number
blinking in inverse text to denote the ‘active’ variable. The and keys move the ‘active’ character
(or cursor) between the different variables within the program while the and buttons respectively
decrease or increase the value of whichever variable is active. Each press of the button will change the
variable’s value by one increment. When the or buttons are held down, the variable will advance
rapidly.
Active Variable System Status Display
Program: XX CLOSE DOOR or READY TO START
S RUN SPEED rpm
T RUN TIME
C CYCLES
D CYCLE DWELL
If the run is stopped or paused, the system status will read “RUN PAUSED.”
At the end of the program, “REMOVE SAMPLES” will be displayed followed by
“PROGRAM DONE.”
PROG >04 20°C
S=4000 C=02
T=0:10 D=X:XX
READY TO START


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PLEASE NOTE:
Homogenization settings cannot be changed once the run is in progress.
For high throughput purposes, a rest or break period is not required.
The user can pause or stop a run at any time by pressing the “Stop/Pause” button once. The unit
displays "RUN PAUSED." At this point, the program can be continued by pressing "Start/OK" once
more. To terminate the program, press the "Stop/Pause" button twice and the unit will default back to
the original program screen display.
2.8 ADJUSTING PARAMETERS
Use the and arrow keys to move the ‘active’ character (or cursor) between the different variables
within the program. The and keys respectively decrease or increase the value of whichever
variable is active.
Adjusting the Speed
Use the and arrow keys to highlight the “S”- speed option. 
Change the speed value using the and arrow keys. The speed adjustment ranges from 500 rpm to
5,000 RPM in increments of 100 RPM. When the desired value is reached, the user can go to the next
desired variable, or return to program ("PROG") to begin the run.
PROG 04 20°C
S=>4000 C=02
T=0:10 D=0:10
RUN SPEED rpm
W
ARNING: Wait until the unit stops and displays “REMOVE SAMPLES
—
PROGRAM
DONE” before opening the door.
CAUTION: To prevent tube breakage at high speeds, specialized bead tubes are required with
speeds higher than 4,200 RPM.

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Adjusting the Run Time
Use the and arrow keys to highlight the “T”- time option.
The user can change the cycle time using the and arrow keys. The run time ranges from 1 second
to 9 minutes and 59 seconds in increments of 1 second. When the desired value is reached, the user can
go to the next desired variable, or return to program ("PROG") to begin the run.
Adjusting the Number of Cycles
Use the and arrow keys to highlight the “C”- cycles option. 
The user can change the number of cycles using the and arrow keys. The number of cycles ranges
from 1 to 10. When the desired value is reached, the user can go to the last variable, or return to
program ("PROG") to begin the run.
PROG 04 20°C
S=4000 C=02
T=>0:10 D=0:10
RUN TIME
PROG 04 20°C
S=4000 C=>02
T=0:10 D=0:10
CYCLES

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Adjusting the Pause or “Dwell” Between Cycles
Use the and arrow keys to highlight the “D”- pause/dwell option.
The user can change the dwell time between 2 or more cycles using the and arrow keys. The
dwell time between cycles ranges from 1 second* to 9 minutes and 59 seconds in increments of 1
second. Once all variables have been programmed, return cursor (>) to "PROG" and press
"Start/OK". The screen will then display "HUBLOCK ENGAGED?".
Ensure that the hub lock switch on the finger plate is securely positioned in the "Lock" position. Press
"Start/OK" once more to begin the programmed run.
Samples can be removed from the PowerLyzer™24 Homogenizer immediately after the run has ended.
*Dwell minimum varies depending upon the speed and length of cycle.
PROG 04 20°C
S=4000 C=02
T=0:10 D=>0:10
CYCLE DWELL
PROG 04 20°C
S=4000 C=02
T=0:10 D=>0:10
HUBLOCK ENGAGED?
W
ARNING: The carousel can become very hot during operation. Use caution when handling the
tube holder.

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2.9 RECOMMENDED SETTINGS
BEAD MATRIX SETTING APPLICATION
Sample Name and Type Lysing matrix Speed – number of cycle x time - pause Note
MICROBIAL Bacteria
Garnet 0.15mm 2,000
–
1 x 5 min
–
0s DNA isolation
Glass 0.1mm 2,000
–
1 x 5 min
–
0s DNA isolation
ANIMAL
Muscle Ceramic 2.8mm 3,500
–
2 x 45 sec.
–
30 sec. RNA isolation
Brain Ceramic 2.8mm 3,500
–
2 x 45 sec.
–
30 sec. RNA isolation
Live
r
Ceramic 2.8mm 3,500
–
2 x 45 sec.
–
30 sec. RNA isolation
Kidney Ceramic 2.8mm 3,500
–
2 x 45 sec.
–
30 sec. RNA isolation
Lung Ceramic 2.8mm 3,500
–
2 x 45 sec.
–
30 sec. RNA isolation
Heart Ceramic 2.8mm 3,500
–
2 x 45 sec.
–
30 sec. RNA isolation
Spleen Ceramic 2.8mm 3,500
–
2 x 45 sec.
–
30 sec. RNA isolation
PLANT
Leaves Ceramic 2.8mm 4,200
–
2 x 45 sec.
–
30 sec. RNA isolation
Leaves Metal 2.38mm 2,000
–
1 x 3 min DNA isolation
Seeds Metal 2.38mm 2,900
–
1
x
3 min DNA isolation
Stems Ceramic 2.8mm 4,200
–
2 x 45 sec.
–
30 sec. RNA isolation
Stems Metal 2.38mm 2,200
–
1 x 3 min. DNA Isolation
Roots Ceramic 2.8mm 4,200
–
2 x 45 sec.
–
30 sec. RNA isolation
Root Metal 2.38mm 2,500
–
1 x 3 min DNA isolation
Flowers Ceramic 2.8mm 4,200
–
2 x 45 sec.
–
30 sec. RNA isolation
SOIL Soil
Garnet 0.7mm 2,000
–
1 x 5 min DNA isolation
Glass 0.1mm (clay) 4,000
–
1 x 45 sec.
–
0s
(loose, high humic) 2,500 – 1 x 45 sec. – 0s DNA isolation