Agilent Technologies G2545A Use and care manual

Agilent G2545A Hybridization Oven
Calibration Procedure
Step 1. Set up and heat the oven 3
Step 2. Start the digital recording thermometer 4
Step 3. Retrieve the temperature data from the digital recording
thermometer 7
Step 4. Calibrate the oven 9
The Agilent G2545A Hybridization Oven maintains temperature calibration
for at least 3 months under normal operating conditions when properly
installed within the laboratory.
See the Agilent G2545A Hybridization Oven Installation, Operation, and
Maintenance Guide (p/n G2545-9001) for information on site
requirements and oven placement.
Calibrate the Agilent G2545A Hybridization Oven:
•After first installation in a working environment
•After each 3 months of use
•When systematic high microarray background noise may indicate a drift
in oven temperature
Do the calibration steps in this guide to maintain optimal performance for
your Agilent G2545A Hybridization Oven.
To view a demonstration of the hybridization oven
calibration procedure, go to
http://www.agilent.com/genomics/protocolvideos, or scan this
QR code with your mobile device.

2 Hybridization Oven Calibration Procedure
Required Parts and Tools
Part Vendor and part/model
hybridization oven Agilent p/n G2545A
hybridization oven rotator rack Agilent p/n G2530-60029
hybridization chambers Agilent p/n G2534A
Fourtec MicroLite II USB digital recording
thermometer or equivalent
•Range -40°C to 80°C
•Resolution 0.1°C
•Accuracy ±0.3°C
MicroDAQ.com
•p/n LITE5008P (8,000 readings)
•p/n LITE5032P (32,000 readings)
Computer and software to work with the digital
recording thermometer
Fourtec DataSuite Software or other software
as specified by the digital recording
thermometer vendor
Calibration service to 65°C for digital recording
thermometer
MicroDAQ.com p/n CAL-NIST-TEMP or other
service as recommended by digital recording
thermometer vendor
general-purpose labeling tape or
2 cable ties (long enough to attach the digital
recording thermometer to the oven rotator rack)

Step 1. Set up and heat the oven
Hybridization Oven Calibration Procedure 3
Step 1. Set up and heat the oven
1Install the hybridization oven rotator rack into the hybridization oven.
2Load the hybridization oven rotator rack with the typical number of
hybridization chambers used during a single hybridization run.
Install the hybridization chambers without microarrays. Distribute the
hybridization chambers across hybridization oven rotator rack positions
to mimic normal operating procedure.
3Turn on the hybridization oven, set rotational speed control to 20 rpm,
and set oven temperature consistent with the recommended
hybridization temperature for the protocol that you use.
4Allow the hybridization oven to heat and stabilize for at least 3hours.

Step 2. Start the digital recording thermometer
4 Hybridization Oven Calibration Procedure
Step 2. Start the digital recording thermometer
•Set up and start the digital recording thermometer.
The software instructions are for the Fourtec MicroLite II USB digital
recording thermometer. The hardware instructions are the same for all
digital recording thermometers.
o
1Install the Fourtec DataSuite Software. Follow the installation
instructions that are included with the software.
2Click DataSuite to start the Fourtec DataSuite Software. The program
opens in Map View.
3Remove the cover on the Fourtec MicroLite II USB digital recording
thermometer and insert the digital recording thermometer into a USB
port on the computer.
4Check that the Fourtec MicroLite II USB digital recording thermometer
is detected by the computer.
When the Fourtec MicroLite II USB digital recording thermometer is
connected to the computer for the first time, the LCD on the digital
recording thermometer displays “Hello”, the firmware version, and then
“Stop”.
If the Logger icon does not appear in the DataSuite program, connect
the digital recording thermometer to a different USB port.
5Right-click the Logger icon and click Setup.

Step 2. Start the digital recording thermometer
Hybridization Oven Calibration Procedure 5
6Set Temperature Scale to °C.
7Mark the Timer Run check box
and select the date and time to
start the recorder.
8Set Interval to 00:05:00 (5
minutes).
9Click Setup.
10 Click Yes to continue.
11 Remove the unit from the USB
port and replace the cover.
12 Put the digital recording
thermometer onto the middle of
the hybridization oven rotator
rack.
13 Secure the digital recording
thermometer to the hybridization
oven rotator rack. Use
general-purpose laboratory
labeling tape or cable ties.

Step 2. Start the digital recording thermometer
6 Hybridization Oven Calibration Procedure
14 Close the oven door and allow the digital recording thermometer to
record temperature data for at least 3hours.
Make sure the hybridization oven rotator rack is set to rotate during
this time.
15 Remove the digital recording thermometer from the oven. Let the oven
continue to operate after you remove the digital recording thermometer
from the oven.
CAUTION Make sure that the digital recording thermometer is secure and will not detach from
the rack. If the digital recording thermometer comes loose during use, the data
collected can be inaccurate. A loose digital recording thermometer that becomes
trapped and prevents the rotator rack from rotating can damage the oven.
WARNING The digital recording thermometer can be hot to the touch. Use caution while
handling.

Step 3. Retrieve the temperature data from the digital recording thermometer
Hybridization Oven Calibration Procedure 7
Step 3. Retrieve the temperature data from the digital
recording thermometer
•Follow the instructions for the digital recording thermometer to retrieve
the temperature data.
These steps are for the Fourtec MicroLite II USB digital recording
thermometer.
1Start the Fourtec DataSuite Software.
2Remove the cover on the Fourtec MicroLite II USB digital recording
thermometer and insert it into a USB port on the computer.
3Right-click the Logger icon and click Stop to stop data recording.
4Right-click the Logger icon and click Download Data.
The Fourtec DataSuite Software automatically saves the data on the
computer.
5Double-click the Logger icon to view a graph of the data.
6To view archived data:
aClick File > Open.
bIn the Open Data Files dialog box, select the time period and logger
for which you want to view the data.
cClick OK.
The archived data appears in History View. The Data File Path shows
the folder in which the logger data was saved.
7To export the data to a spreadsheet, click Export to Excel in the
lower graph toolbar.
8Record the oven temperature from the digital recording thermometer.

Step 3. Retrieve the temperature data from the digital recording thermometer
8 Hybridization Oven Calibration Procedure
Figure 1 Temperature reading. Period of stable temperature is highlighted.
Take the temperature reading from the period of stable temperature data
recorded before the oven door is opened.

Step 4. Calibrate the oven
9 Hybridization Oven Calibration Procedure
Step 4. Calibrate the oven
1Compare the temperature reading from the digital recording
thermometer with the temperature on the digital display on the control
panel of the hybridization oven.
Make sure that the oven temperature is stable after the digital recording
thermometer is removed and before you adjust the hybridization oven.
The oven temperature is stable when the temperature display on the
oven reads the current set temperature.
2If the difference between the two temperature readings is more than
0.2°C:
aPress both ▲and ▼at the same time until the two outside decimal
points of the display begins to flash to put the display into
calibration mode.
bWhile the decimal points are flashing, press the ▲or ▼arrow pad
until the reading on the display matches that measured by the digital
recording thermometer.
If the arrow pads are not pressed for five seconds, the display stops
blinking and reads the temperature in the chamber.
3To recheck the temperature, start from “Step 2. Start the digital
recording thermometer” on page 4. Repeat calibration steps if needed.
The calibration procedure is now complete. Please keep your records as
required by GLP guidelines.

Agilent Technologies, Inc. 2014
Revision A1, February 2014
*G2545-90002*
G2545-90002
www.agilent.com