Myr MYREVA SS-30 User manual

Slide Stainer MYREVA SS-30 and SS-30H
User manual
Version 07/17

Especialidades Medicas Myr, S.L. would like to thank you for the purchase of this
equipment and for your confidence in choosing our company.
We are committed to offer you the best and most reliable service.
Don’t hesitate to contact your local dealer for any question related to this Slide Stainer.
Your Especialides Médicas Myr, S.L. team

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3
Contents
Introduction ................................................................................................................... 4
Intended use .......................................................................................................... 4
Warranty ................................................................................................................ 4
Certification ............................................................................................................ 5
Safety ............................................................................................................................. 5
Safety Precautions ................................................................................................ 6
Hazards ................................................................................................................. 6
Documentation ...................................................................................................... 8
Conditions for the transportation of the instrument ................................................ 8
Technical data ............................................................................................................... 9 
Reception and installation of the unit ....................................................................... 10
Connection .......................................................................................................... 11
Connecting to the mains............................................................................. 11
Water inlet and outlet ................................................................................. 12
Rinsing trough water connection ................................................................ 12
Commisioning ............................................................................................................. 13
Introduction .......................................................................................................... 13
Slide stainer components .................................................................................... 16
Display screen and keypad ................................................................................. 16
Menus and options .............................................................................................. 17
Main menu ................................................................................................. 17
Configurations and settings ........................................................................ 18
Advanced configurations ............................................................................ 21
Programming .............................................................................................................. 22
Preparation .......................................................................................................... 22
List of reagents ........................................................................................... 22
Preparation of programmes ........................................................................ 23
Reagent map .............................................................................................. 26
Reagent and programme editing ......................................................................... 27
Editing reagents list .................................................................................... 27
Entering programmes ................................................................................. 30
Programming with PC .......................................................................................... 31
Connecting the equipment ......................................................................... 31
Installing the programme ............................................................................ 31
Running the software ................................................................................. 31
Configuration .............................................................................................. 32
File menu ................................................................................................... 33
Creating a programme ............................................................................... 34
Reading and writing programmes .............................................................. 35
Tabs “Info, Settings and Logs” ................................................................... 38
“History” tab (Only SS-30H) ....................................................................... 39
Running a process ..................................................................................................... 42
Loading procedure ............................................................................................... 42
Monitoring the process ........................................................................................ 45
Unloading procedure ........................................................................................... 46
Alarm and attention messages ............................................................................ 47
Battery operation ................................................................................................. 48
Maintenance ................................................................................................................ 49
Routine maintenance ........................................................................................... 49
Cleaning .............................................................................................................. 49
Replacing the filter...................................................................................... 50
Disposal of the instrument after final shutdown ..................................................... 51
Replacement part codes for the SS-30 slide stainer ............................................... 51
Programming form ..................................................................................................... 52
Reagent map ............................................................................................................... 53


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Introduction
The Slide Stainer MYREVA SS-30 is an automatic programmable device
for Cytology and Histology processes. It is composed of a metallic chassis
holding reagents troughs, washing troughs, robotic arm X/Y and electric
fun with active charcoal filter. The instrument also features a transparent
cover with three hinged doors.
Before operating the instrument, please read these instructions carefully
to familiarize yourself with its proper operation and functions.
The Slide Stainer is a highly efficient, easy to handle device, developed
taking into consideration the practical needs of professionals.
Only skilled or specially trained personnel must operate the instrument.
The marked safety measures as well as the regulations of your respective
lab must strictly be observed.
Intended use
The MYREVA SS-30 is a high-performance automatic slide stainer used
exclusively for the staining of histological, cytological and haematological
samples.
No use other than that indicated in this manual is permitted. Its use fo
r
purposes other than those specified shall result in the automatic invalidation
of the manufacturer’s warranty.
Warranty
The Myr product is warranted against defects in material and workmanship
for a period of 1 year.
Parts which prove to be defective during the warranty period will be repaired
or replaced free of charge.
No other warranty is expressed or implied.
Unauthorized modification or repair by third party persons will void the
warranty.
The warranty will expire in case of improper or wrong use of the instrument
and in case the warning and precautionary messages are not observed.
Especialidades Médicas Myr, S.L. is not liable for any occurring damage.
Once the guarantee period has expired, a maintenance contract should be
signed to ensure the unit is kept in optimum operating condition. For more
information on maintenance contracts, please contact your local distributor.

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Certification
Especialidades Médicas Myr, S.L. certifies that this instrument has been
tested and checked carefully. All technical data were checked before
delivering to guarrantee that fulfill published specifications.
The instrument complies with applicable international safety regulations.
Safety
The installation and normal use of the Slide Stainer is simple and safe if you
observe the instructions given in this manual.
Should the equipment be used for unauthorised purposes or uses not
specified by the manufacturer, safety may be impaired.
Input and output circuits are isolated from the main power supply.
However, those situations which could be constitute a risk for laboratory
personnel or equipment, are distinguished in this manual with the following
symbols and warning messages:
This sign, symbolizing DANGER, means that injury to persons as well as
material damage to the unit may occur if these instructions are not observed.
For your own safety, observe these instructions carefully.
This sign, symbolizing CAUTION, means that damage to the unit may occu
r
if these instructions are not observed.
For a long service life of the unit, observe these instructions carefully.
The symbol shown here indicates areas where the temperature may
exceed 60°C
This INFORMATION sign draws the user’s attention to important details and
additional information about the device, and should therefore especially be
taken into account.
All persons who are required to operate and programme the Slide Staine
r
should read and fully understand these instructions before using the device.
Warning of biological danger.
Warning of radioactive danger.

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Safety Precautions
The operator's safety is affected, when the instrument is not operated in
accordance with this instruction manual.
Apart from the instructions given in this manual, the personnel involved in
operating the Slide Stainer should know and observe the general guidelines
and rules for safety and hygiene applicable to the workplace where the unit
is installed.
Please observe the following general precautions during operation of this
instrument. Failure to comply with these precautions violates safety
standards and the intended use of the instrument. Especialidades Médicas
Myr, S.L. is not liable for misuse of the instruments and failure to comply
with basic safety requirements.
Hazards
Instrument grounding
The instrument is supplied with a 100/240 V mains adapter with a 12V
output transformer . The power outlet must be connected to the protective
earth and must meet the International Electrotechnical Commission (IEC)
regulations.
Danger in explosive environment
The instrument must not be operated in the presence of flammable gases.
Moreover, the instrument must not be exposed to conditions whereby
dangerous gas concentrations can occur.
A
s there is a risk of fire if working with an open flame (e.g. Bunsen burner)
near the equipment (solvent vapours), a minimum distance of 2 metres
between the flame and the slide stainer should be maintained.
Hazard of radio-active radiation
When working with radioactive specimens observe all applicable radiation
safety procedures. When working with radioactive contaminated material,
appropriate safety and disinfecting measures must be carried out. According
to the rules and regulations concerning the handling of radioactive
contaminated material of the respective laboratory, safety clothing (e.g.
particle mask, gloves, protective shoe covers) must be worn. Radioactive
contaminated waste must be disposed of according to the respective
regulations.
Chemical hazard
When working with the Slide Stainer, sometimes it is necessary to handle
flammable, dangerous fluids. Only trained and qualified laboratory
professionals, being aware of the potential dangers and being capable o
f
handling those fluids properly, are allowed to use the instrument.
Before handling dangerous liquids, you must make sure to have read and
understood the MSDS and specifically understood the safety instructions and
the instructions for proper disposal.
Wastewater treatment
A
ccording to environmental regulations, the occurring waste water should be
drained into a discharge channel with subsequent biological/chemical
purification.

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When the instrument is not in operation, the reagent troughs
should be covered with the plastic lids supplied to minimise
reagent evaporation.
Fume accumulation
Remember that, when working with the device, reagent fumes that are
harmful to health always form. The Slide Stainer should always operate with
the active carbon filter fitted or the discharge duct connected to a fume
extraction system.
To prevent the accumulation of fumes that can damage the internal
components of the machine: NEVER TURN OFF THE MACHINE WITH
THE SWITCH WHILE REAGENTS REMAIN INSIDE. Leave the machine
in sleep mode to enable the programmed extraction cycle to be performed.
See page 21.
Hazard of infection
Specimens used during the intended operation of the instrument might
potentially be infectious. For this reason, it is recommended to observe the
general laboratory regulations concerning protection against danger o
f
infection. Information on decontamination media, their use, dilution and
effective range of application can be read in the Laboratory Biosafety Manual:
1984 of the World Health Organization.
When working with infectious material, appropriate safety and disinfection
measures must be carried out. According to the rules and regulations
concerning the handling of infectious material of the respective laboratory,
safety clothing (e.g. particle mask, gloves, protective shoe covers) must be
worn. Infectious waste must be disposed of according to the respective
regulations.
Burn hazard
The drying station can reach high temperatures. Take care not to get burned.
Hazards associated with faults caused by electromagnetic interference
To avoid the hazard of malfunction of an instrument, it must only be operated
in a controlled electromagnetic environment. This means that transmitters
such as mobile phones must not be operated in their close vicinity.
In case of malfunctions and/or service work, please turn off the instrument
and contact your local dealer.
Hazards associated with the device’s moving parts
Due to the potential hazards associated with the operation of devices with
moving parts, the Slide Stainer must only be used by professionals o
r
properly-trained personnel. We recommend that the unit should not be
handled with the cover open, except during loading and unloading, and in
accordance with the safety conditions detailed below. To avoid danger,
moving speed has been reduced.

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Documentation
This instruction manual will be supplied together with each instrument.
Further copies can be ordered at the Technical Service Address by giving
the serial number of the instrument, the version of the instruction manual
and the date of issue.
This instruction manual is available in English and Spanish
Errors and omissions excepted. Subject to amendment and improvement
without further notice.
Conditions for the transportation of the instrument
Repair or maintenance work are normally carried out at the site o
f
installation. If this is not possible for some special reasons, the instrument
can be returned to Myr. The contact address can be found at the end of this
instruction manual.
For transportation outside closed buildings use the original packing.
If the original packing is no longer available, please contact your local My
r
representation.

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Technical data
General information
Power Requirements
Through a transformer:
Input: 100 – 240V , 1.5A, 50 - 60Hz
Outpu: 12V , SS-30: 5.5A — SS-30H: 12.5A
Water supply
Supply:
Running water
Maximum pressure: 4 bar
Water temperature: Less than 30°C (86°F) without freezing
Fitting: Standard ¾”
Drainage:
By gravity
Pipe diameter: 20 mm
Classification in accordance with
IEC 1010
Protection class 1
Pollution degree 2
Overvoltage category II
Operating conditions
Operating temperature range: 10 - 40ºC
Relative air humidity: 10 - 80% non-condensing
FOR INDOOR USE ONLY
Dimensions and weight
Dimensions 1200 x 440 x 368 mm (W x D x H)
Weight when unloaded and
without packaging 55 kg
Total weight with packaging 92 Kg
Transportation and packaging
conditions Temperature: -20°C to +50°C
Operating capacity
Processing capacity
Up to 5 racks at a time, depending on the programmes, load
frequency and device configuration.
Simultaneous performance of up to 5 different staining protocols
Load capacity per rack 30 slides
No. of programmes in the
memory Stores up to 20 programmes, each with up to 50 steps
No. of reagents in the memory Maximum 52 (49 user-configurable)
Immersion time 1 s - 59 m 59 s per step.
Number of stations 20
Reagent stations Maximum 18
Reagent tray volume 300 ml
Cleaning stations Maximum 3
Loading stations Maximum 2
Unloading stations SS-30: Maximum 3
SS-30H: Maximum 3 (2 with drying chamber active)
Fume extraction Active carbon filter
Battery life
2 hours (lithium-ion batteries)
Only batteries approved by the manufacturer are
permitted to be used

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10
Reception and installation of the unit
Before removing the Slide Stainer from their shipping package, carefully
inspect the wooden case for any damage which may have occurred during
transport. Should you detect any sign of damage, do not open the case and
immediately report the situation to the transport agency.
A
fter removing instrument from its package, inspect it carefully for damage.
Should any be noted, immediately notify the distributor from whom the unit
was purchased.
Remove the protective pieces and elements used for transportation. Please
keep the original packaging and protective pieces in case the device needs
to be returned.
Check that the following items have been supplied:
Items included in the box  Basic device SS-30
 20 reagent troughs
 1 power supply with transformer
12V 5,5A

Basic device SS-30H
 18 reagent troughs
 2 drying troughs
 1 power supply with
transformer 12V 12,5A
 3 complete water troughs with fittings.
 3 water supply tubes with fittings for water troughs
 1 tool to cut the trough water supply tubes
 23 plastic lids for the reagent troughs
 3 racks + supports (30 slides)
 1 active carbon filter
 1 mains adapter with transformer 12V 5A
 1 power cable
 1 USB cable for connection to PC
 1 water supply hose (1.5 m) complete with ¾” connection fittings
 1 corrugated drain hose (3 m)
 1 set of clips for drain hose
 1 spirit level
 1 user manual
Remove the protective elements securing the robotic arm. Do not attempt to
move it manually. The robotic arm will move to its starting position the first
time the instrument is turned on.
When choosing where to install the instrument, as well as its dimensions, also
take into account its weight. See the dimensions and weight on page 9.
The installation site must be well ventilated and free from any flame or source
of sparks.
Place the instrument on a stable and level surface. Use the spirit level
supplied to ensure that the Slide Stainer is installed to facilitate the flow o
f
water to the drainage area.
Make sure that there is enough space to fully lift the covers and allow air to
circulate.

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The fume extractor outlet is located at the back of the instrument. For correct
ventilation of the instrument a minimum space of 100 mm from wall should
be kept free.
Connection
Connecting to the mains
CAUTION
Electronics of the Slide Stainer are protected and screened. However, the
instrument should not be sited near to heavy electrical machinery
generating heat, vibrations or strong electrical noises.
The slide stainer is supplied with a power cable and transformer to be
plugged into a standard socket with earth connection. The power supply
socket is on the right-hand side of the device.
1 Power switch
2 USB port
3 Power supply socket
Mains adapter USB cable

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12
Water inlet and outlet
The water inlet is connected to a standard household appliance hose with
a ¾” threaded fitting supplied with the device. The water supply must be
equipped with a stopcock (not supplied) to enable it to be turned off.
Myr accepts no responsibility for the connection between the water supply
and the equipment.
The hose supplied for connection to the water outlet has an inner diamete
r
of 20 mm and must be attached to the outlet nozzle with the hose clip
supplied.
Be sure that the drain level is always at a lower level than the unit and the
hose is always hanging downwards.
Remember to shut off the water supply after working with the device.
Rinsing trough water connection
One of the main features of the Slide Stainer is its modularity and adaptability
to user needs.
The Slide Stainer can have up to three rinsing troughs with water in any
station between 5 and 17.
To determine in which stations the water troughs should be located, consult
the person responsible for programming or the pathologist, who will
determine the optimum positions for the type of process to be performed.
In stations 18, 19 and 20, there are three water outlets with rapid self-locking
fittings, which have to be connected, with the flexible hoses supplied (cut to
a suitable length), to the troughs in the stations that have been selected as
rinsing troughs.
The length of the tube (L) will depend on the position of the water intake
station and the destination station.

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13
Length L in mm is calculated with the formula: (Nt - Nc) * 55 - 16
Nt is the number of the station in which the water inlet is located.
Nc is the number of the station containing the trough to be connected.
55 is the distance in mm between the troughs and water inlets.
16 is the measurement in mm taken up by the fittings.
It is recommended that you use the outlet of station 18 to feed the station
closest to the beginning, station 19 for the middle one and station 20 for the
one nearest the end.
Remember that the way that the water troughs are arranged will be
determined by the processes to be carried out in order to optimise the
performance of the Slide Stainer.
For the example processes shown here, the optimum stations are 6, 8 and
10, which have lengths L of 644 mm, 589 mm and 534 mm respectively. See
the Reagent map on page 23.
Commisioning
Introduction
If you are using the Slide Stainer for the first time, before you start any real
staining processes, you are strongly advised to familiarise yourself with the
handling and programming of the device. To do so, we suggest that you
perform some process simulations without samples or reagents. You can
allow the water troughs to be filled, which will also verify that they can fill up
and drain correctly.
Before starting to work with the Stainer, it is advisable to perform a status
check. Check the following:
Power Requirements
Check that the power switch is on and that voltage is reaching the device.
The window in the bottom left-hand corner illuminates, except when it is
being powered by the backup battery (for more information about
autonomous operation with the backup battery, see page 48). The display
screen also illuminates.
Water inlet and outlet
Check that the stopcock for the device’s water supply is turned on and the
drain connected. On page 20, you will find the procedure for forcing the entry
of water into the previously-connected troughs and verifying that it is
functioning correctly.
Troughs
The Slide Stainer is equipped with 20 stations, which correspond to the
location of 20 troughs. The first ones are used for loading racks and the last
ones for unloading.
SS-30H Station 20 of the SS-30H slide stainer can also be used as a
drying/dewaxing station. For this, it features a special trough (marked with
an H) with a side opening to enable the samples to be dried (see next page).

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Check that the 20 troughs are positioned correctly and not inverted. If they
are not positioned correctly, significant mechanical problems can occur.
To prevent the reagents from overflowing, the troughs should only be filled
up to the level indicated on the inner recess.
Note that the reagent that each trough should contain is determined by the
previously-established Reagent map. See page 23.
The number of loading troughs can be set at 1 or 2 and unloading troughs
at:
Model SS-30 Model SS-30H
1, 2 or 3 1, 2 or 3 (without drying trough)
1 or 2 (with drying trough)
Only SS-30H When you use the drying trough in station 20, and given that it cannot in
this case be used as an unloading station, place an empty trough into
station 19 and programme it as an unloading station from the programmes
that use drying station 20.
The drying trough is labelled with an “H”. Make sure that it always occupies
station 20 if it is used as a drying/dewaxing station.

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Only SS-30H
Pay special attention to the programmes that use the drying station. In these
cases, make sure that the previous programmed step cannot originate from
a trough containing a flammable reagent (e.g. xylol). This is to prevent a
possible fire.
Robotic arm
Check that the robotic arm is located on the left-hand side of device and in a
raised position (starting position). If not, press F key to situate the arm in the
initial position.
Rack collection.
To check that the robotic arm is collecting the racks properly, see the
MOVEMENT procedure on page 20.

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Slide stainer components
The figure below shows the Slide Stainer’s different components.
1. Loading door
2. Central door
3. Unloading door
4. Robotic arm
5. Slide rack
(maximum 5)
6. Troughs (20)
7. Display screen and
keypad
Display screen and keypad
The DRAIN key is used to enable or disable the draining of the racks as
they leave each trough. If enabled, the word DRAIN appears on the
monitoring screen.
This function can only be enabled or disabled when the Stainer is not
performing any processes.
The F key is used to abort all staining processes that have been started
and to situate the arm in the initial position with the equipment in standby
mode and away from the process screen.
The LOAD key is used to confirm the steps of the loading process.
The UNLOAD key is used to confirm the steps of the unloading process.

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The arrow keys are used for navigation (option selection) within the
different menus, and the middle key is used to confirm or validate the
selected option. It is equivalent to the ENTER key.
The ESC key is used to exit the various menus in which values and
operation options are entered.
Menus and options
Main menu
Slide Stainer
V X.X
MAIN MENU
STAIN EDIT SETUP S.A.T.
The main menu shows the four main
options.
Use the arrow keys to highlight the
option you wish and press ENTER.
STAIN
This is the normal operation option for performing processes. It enables
the user to select the programme to run, control loading and unloading o
f
the racks in the troughs and view current processes.
EDIT
This allows you to enter and edit programmes with the keypad.
Remember that you can also edit programmes with a PC and then transfe
r
them to the Stainer via a USB.
SETUP
This enables you to adjust the stainer settings, such as language, date,
speed, dip, etc.
S.A.T.
Technical Support Service: This provides access to advanced functions
which must only be modified by authorised personnel.

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18
Configurations and settings
STAIN
Before starting the first staining process, some parameters have to be
configured in the EDIT and SETUP menus.
Once the settings have been adjusted and the reagents and programmes
edited, the STAIN process can be started. See the details of how to run a
staining process on page 27.
EDIT REAGENTS
This enables you to enter a numbered list with the reagents used.
This list is merely informative and is used to facilitate the entering
of the names of the reagents during programme editing.
VIEW/
EDIT
Enables the entering of the reagents to be used,
numbered 1 to 52. See an example reagent list on
page 22.
DELETE
This enables you to selectively delete reagent
positions.
Only in SS-30H
MAP
Enables each station to be assigned with a reagent
and, optionally, its useful life in days and/or operating
cycles (racks processed) to be tracked. See page 28
PROGRAMMES
Before starting a staining process, the Stainer needs to be
provided with the sequence of steps that each rack must follow.
The Programming Forms must be previously defined. See page
23.
VIEW/
EDIT
This enables you to enter programmes.
View the editing procedure in detail on page 27.
DELETE
This enables you to selectively delete programmes.
SETUP
Some of these settings can only be configured during initial device start-up
(e.g. language, clock).
Others will only require changes if so decided by the pathologist
responsible for the procedure (reagents, dip, speed, etc.)
A
lso included here are the parameters that control the fume filter and
ventilation.
Each option includes a brief summary of its function.
LANGUAGE
Use the arrow keys to select the language and then press ENTER.
SPANISH
ENGLISH
CLOCK
Use the arrow keys to select each parameter and then press ENTER.
Within each option, use the arrow keys to increase or decrease the value
and then press ENTER to confirm.

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19
Clock edit menu
Edit date: DD/MM/YY
Edit hour: HH:MM:SS
Type: 12/24H
DIP
Dip of the rack within the trough.
The dip height, dips number and dip speed of the rack in the reagent and
washing troughs can be adjusted.
REAGENT
This affects the reagent stations.
It is possible to define up to 4 Dip Modes, each with separate
parameters.
With the arrows, select the Dip Mode to be defined
(01... 04).
Press ENTER to access each of the parameters and select
the value using the and arrows.
The example shows the editing of Mode 01 parameters for the
reagent stations.
REAGENT S. - [MODE 01]
Dip Height (mm.) 20
Dips Number 4
Dip Speed 2
Dip Height (mm.) 10-20-30
This value determines the vertical travel of the rack during dip
within the trough. The standard value is 20 mm.
Dips Number: 1-2-3-4-5-6-7-8-9-10
This value determines the number of times the rack will be
moved during dip within the reagent trough. The standard value
is 4.
Dip speed: 1-2-3-4
Please note: value 1 is the highest speed and value 4 the
lowest. The standard value is 2.

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20
CLEANING
This affects the washing stations.
With the arrows, select the Dip Mode to be defined
(01... 04).
Press ENTER to access each of the parameters and select
the value using the and arrows.
The example shows the editing of Mode 01 parameters for the
washing stations.
CLEANING S. - [MODE 01]
Dip Height (mm.) 40
Dips Number 4
Dip Speed 2
Dip Height (mm.) 20-30-40-50-60
This value determines the vertical travel of the rack during dip
within the trough. The standard value is 40 mm.
Dips Number: 1-2-3-4-5-6-7-8-9-10
This value determines the number of times the rack will be
moved during dip within the reagent trough. The standard value
is 4.
Dip Speed: 1-2-3-4
Please note: value 1 is the highest speed and value 4 the
lowest. The standard value is 2.
SPEED
This option enables the speed of the vertical movement of the rack to be
determined.
Speed edit menu
Lifting speed: 1-2-3-4
Lowering speed: 1-2-3-4
Please note: value 1 is the highest speed and value 4 the lowest. The
standard value is 4 for raising and 3 for lowering.
Note that the slowest elevation speeds (e.g. 3, 4) produce less reagent drag.
WATER
Water flow menu
Water valve (closed/open)
This enables you to manually open or close the solenoid valve that controls
the entry of water into the Stainer. It is used to check trough filling time and to
ensure that water flow does not exceed draining capacity. Check that the fillin
g
time is about 13 seconds per trough.
Valve time: MM:SS
This indicates how much time has elapsed with the valve open.
Rinsing Time: 0-30-60-90
This determines how long water will continue circulating in the rinsing trough
s
after the rack leaves the trough.
MOVEMENT
This option allows you to check the functioning of the robotic arm. Select
Setup > Movement.
The device requests you to place a rack in station 1 and to confirm the
placement by pressing ENTER to perform a rack collection cycle. Press
ESC to cancel the option.
If the rack is not collected properly, notify the person responsible for the
device’s maintenance.
Only in SS-30H
DRYER
Drying/dewaxing station menu
Status (ON/OFF)
Enables the drying station to be activated/deactivated.
Temperature
Sets the drying temperature
Celsius/Fahrenheit
Sets the temperature scale as Celsius or Fahrenheit