Nano Temper Prometheus Panta User manual

Prometheus Panta
User manual

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Table of contents
1. About this user manual......................................................................................................................... 5
Directions for more detailed information.................................................................................... 5
2. Safety information................................................................................................................................ 6
Symbols and descriptions ............................................................................................................ 6
Use and misuse............................................................................................................................. 7
Safety instructions........................................................................................................................ 7
3. The Prometheus Panta system ............................................................................................................ 9
General.......................................................................................................................................... 9
3.1.1. Intended use ................................................................................................................................. 9
3.1.2. Conformity .................................................................................................................................... 9
3.1.3. Identification................................................................................................................................. 9
Technical information ................................................................................................................ 10
3.2.1. Technical specifications ............................................................................................................. 10
3.2.2. Connections for input and output.............................................................................................. 11
Legal............................................................................................................................................ 12
Limited warranty ........................................................................................................................ 13
4. Prometheus Panta setup.................................................................................................................... 14
Scope of delivery......................................................................................................................... 14
Unpacking................................................................................................................................... 14
Startup ........................................................................................................................................ 14
Cleaning ...................................................................................................................................... 14
Software updates........................................................................................................................ 15
5. Prometheus Panta instrument........................................................................................................... 16
Temperature Range.................................................................................................................... 16
Capillary Format ......................................................................................................................... 16
6. Using Prometheus Panta.................................................................................................................... 17
General Usage............................................................................................................................. 17
Sample Loading.......................................................................................................................... 17
6.2.1. Sample Loading for the Prometheus Panta .............................................................................. 17
7. Patents and intellectual property...................................................................................................... 20

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8. Transport and disposal....................................................................................................................... 21
Repackaging for transport.......................................................................................................... 21
Waste disposal ............................................................................................................................ 21
System disposal.......................................................................................................................... 21

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1. About this user manual
This manual is a guide for using Prometheus Panta instrument. It instructs first-time users on how to use
the instrument and serves as a reference for experienced users.
Before using the Prometheus Panta instrument, please read this instruction manual carefully, and make
sure that the contents are fully understood. This manual should be easily accessible to the operator at all
times during instrument operation. When not using the instrument, keep this manual in a safe place. In
case of loss, please contact NanoTemper Technologies customer support
(https://nanotempertech.com/support) for a replacement copy of this manual.
Directions for more detailed information
There are two sources for further, more detailed information on scientific principles and
recommendations for software usage, as well as data interpretation. One is the NanoPedia knowledge
base that is integrated into the PR.Panta Control software. It contains a variety of articles about the
biochemistry of protein stability, the implemented measurement technologies nanoDSF, backreflection
and DLS and the operational functions of Prometheus Panta. A PDF version of the NanoPedia can be
accessed through the NanoTemper Technologies Explorer Community.
The NanoTemper Technologies Explorer Community is an online community to obtain resources for
NanoTemper products such as application-notes, tech-notes or protocols. It is also a place to exchange
best practices directly with other Prometheus users. You also have the possibility to ask questions and
have them answered by NanoTemper support guides. Follow https://nanotempertech.com/be-an-
explorer/ for access to the Explorer Community.

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2. Safety information
To ensure operation safety, this system must be operated correctly. Carefully read this chapter to fully
understand all necessary safety precautions before operating the system.
Symbols and descriptions
This section describes the safety symbols and descriptions used in this manual, as well as the labels on
the system.
Please take a moment to understand what the signal words WARNING! CAUTION and NOTE mean in this
manual.
WARNING!
AWARNING! indicates a potentially hazardous situation which, if not avoided,
could result in serious injury or even death.
CAUTION
ACAUTION indicates a potentially hazardous situation which, if not avoided, may
result in minor or moderate injury. CAUTION may also be used to alert against
damaging the equipment or the system.
Do not proceed beyond a WARNING! or CAUTION notice until you understand the
hazardous conditions and have taken the appropriate steps.
NOTE
ANOTE provides additional information to help the operator achieve optimal
system and assay performance.
Read manual label. This label indicates that you must read the manual before
using the system. This label is positioned on the back of the instrument.
Warning symbol. This symbol indicates a surface that can heat up and cause burn
injuries. This warning label is positioned on the sample tray.
Warning symbol. This symbol indicates a possible risk for hand injuries by crushing
and sharp edges. This warning label is positioned on the sample tray.

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Use and misuse
Use the Prometheus Panta system only after having read and fully understood this user manual. Use the
system only in perfect condition. If the system shows any signs of damage, stop operation and contact
NanoTemper Technologies GmbH.
Do not modify the system in any way. Do not use it for anything other than its intended purpose.
Safety instructions
CAUTION The Prometheus Panta has to be installed in a way that does not hinder access to the external
power supply and its power plug.
CAUTION Connect the Prometheus Panta instrument power supply in a way that avoids tripping hazards.
CAUTION Do not use extension cords. Damaged cords, plugs or cables need to be replaced immediately.
Failure to comply may result in a risk of personal injury or damage to the instrument.
CAUTION Do not operate the Prometheus Panta instrument with a power supply other than the one
delivered by NanoTemper Technologies.
WARNING! Connect the Prometheus Panta to the AC power supply using the supplied power cable.
The AC power supply, which is connected to the Prometheus Panta, must only be connected to an outlet
that has a ground contact, since the instrument is assembled in line with the specifications for safety
class IEC 61010-1:2010.
WARNING! Danger of electric shock, fire and skin burns. Do not open the system (other than operating
the door via the software). Do not reach into the door opening.
WARNING! Do not operate the Prometheus Panta instrument with substances or under conditions that
pose a risk of explosion, implosion or release of gases. Do not use the instrument with hazardous or
infectious substances.
CAUTION Use only aqueous samples for analysis in the instrument.
CAUTION The Prometheus Panta instrument weighs approx. 35 kg. Two people are required for
transport. Moving the instrument alone entails a risk of personal injury or damage to the instrument.
CAUTION Do not open the instrument manually or anywhere other than the sample loading drawer.
Opening entails a risk of personal injury or damage to the instrument and may only be done by
NanoTemper Technologies staff.
CAUTION Only NanoTemper Technologies staff may service the instrument. Turn off the power switch
and unplug the power cord before servicing the instrument, unless instructed otherwise.
CAUTION The instrument contains a UV-LED. The UV-LED emits invisible ultraviolet radiation (UVB
radiation) when in operation, which may be harmful to eyes and skin, even at brief periods of exposure.

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Do not look directly into the UV-LED during operation. If used as intended, the instrument emits no UV
radiation.
CAUTION Mechanical moving parts within the instrument can pinch or injure your hands or fingers. Do
not touch or open the instrument while parts are moving. Do not reach into the opening when the door is
open.
CAUTION The display pane is made of glass. Broken glass can injure your hands or fingers.
CAUTION The sharp edges of the Prometheus Panta thermal element pose a risk of injury to hands and
fingers.
CAUTION The instrument contains a temperature regulator to control the sample temperature. Some
accessible parts of the instrument can reach temperatures of up to 60 °C. Don’t touch the temperature-
controlled parts of the instrument when the temperature controller is set to high temperatures.
CAUTION Do not use the instrument at ambient temperatures below 15 °C.
CAUTION Use the instrument only at noncondensing conditions (20-70 % humidity, 15-30 °C). At very
high humidity levels, even normal operating temperatures may result in condensation and corrosion.
CAUTION Turn off the instrument when not in use.
CAUTION Do not use 100% ethanol or other types of organic solvents to clean the instrument as they
may remove the instrument paint.
CAUTION The instrument contains a laser module (visible
laser radiation class 3B according to IEC 60825-1: 2014).
Lasers or laser systems emit intense, coherent
electromagnetic radiation that has the potential of causing
irreparable damage to human skin and eyes. Direct eye
contact can cause corneal burns, retinal burns, or both, and
possible blindness. Do not attempt to open the instrument as this poses a risk of personal injury or
damage to the instrument. When the instrument is used as intended it emits laser radiation of LASER
CLASS 1.
CAUTION Remove loose parts (capillaries and capillary lid) before transport as they may damage the
measurement optics
CAUTION The heating element contains nickel. If allergic to nickel wear protective gloves when cleaning
the tray surface to avoid skin irritation and damage.
CAUTION If liquids penetrate the instrument, for example due to spillage, stop operation of the
instrument, disconnect it from the power supply and contact NanoTemper Technologies GmbH.
NOTE Insufficient air supply can cause overheating of the system. Assure enough air supply by not
covering or obstructing the back of the system.
NOTE Do not install the instrument in a location where it may be exposed to direct sunlight.

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3. The Prometheus Panta system
General
3.1.1. Intended use
The Prometheus Panta system provides fast and sensitive determination and quantification of
conformational and colloidal protein stability. For this purpose, it performs nanoDSF (nano-differential
scanning fluorimetry), backreflection, and DLS (dynamic light scattering) measurements in glass
capillaries. The system is intended for research purposes only. It is not to be used for diagnostic
purposes.
3.1.2. Conformity
The following safety and electromagnetic standards were considered:
•IEC 61010-1:2010 Safety requirements for electrical equipment for measurement, control and
laboratory use. Part 1 General Requirements
•IEC 61010-2-010:2014 Safety requirements for electrical equipment for measurement, control and
laboratory use. Part 2-010: Particular requirements for laboratory equipment for the heating of
materials.
•IEC 61326-1:2012 EMC, Electrical equipment for measurement, control and laboratory use –EMC
requirements.
•IEC 60825-1:2014 Safety of laser products.
3.1.3. Identification
The identification labels (Figure 1) are positioned at the rear panel of the instrument. They include
manufacturer information, system model name and serial number (SN), electrical requirements, and the
CE conformity symbol.
Figure 1: Identification labels for Prometheus Panta.

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Technical information
3.2.1. Technical specifications
Electricity
Input external power supply
90–264 VAC ± 10 % 47–63 Hz, 230 VA max
Output external power supply
24 VDC, 11.67 A max
Electrical input
24 VDC, 10 A
Overvoltage category
CAT I
Pollution degree
2
Environmental
Operating temperature
15–30 °C (indoor use only)
Humidity
20-70 %, noncondensing
Operating altitude
max 2000 m
Prometheus Panta dimensions
Width
35 cm (13.8”)
Height
51 cm (20.8”)
Depth
55 cm (20.5”)
Weight
35 kg (77 lbs) net
Power supply dimensions:
Width
21 cm (8.3”)
Height
9 cm (3.5”)
Depth
3 cm (1.1”)
Weight
0.5 kg (1.1 lbs) net max
DLS Laser
Wavelength
405 nm ± 5 nm
Power
100 mW max.
Prometheus Panta Laser classification
The device is LASER PRODUCT CLASS 1
Temperature control
Temperature control range
15 °C –95 °C (at 25 °C)
Optional High Temperature Upgrade
15 °C –110 °C (at 25 °C)
Precision of temperature control
± 0.1 °C