NanoPhotometer N50 User manual

NanoPhotometer®NP80/N60/N50/C40
User Manual
Version 3.1
Software Version 3.1.13220

NanoPhotometer®NP80/N60/N50/C40 User Guide Version 3.1
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Declaration of conformity for the NanoPhotometer®(C40/N50/N60/NP80)
This is to certify that the Implen NanoPhotometer®conforms to the requirements of the
following directives:
2014/35/EU Low Voltage Equipment Safety Directive
2014/30/EU Electromagnetic compatibility (EMC) directive
IEC 60529 Protection class IP20
2011/65/EU Restrictions on the use of certain Hazardous Substances
in Electrical and Electronic Equipment (ROHS)
2012/19/EU EC Directive on Waste Electrical and Electronic Equipment (WEEE)
2003/108/EC & 2008/34/EC. By ensuring this product is disposed of
correctly, you will help prevent potential negative consequences for the
environment and human health, which could otherwise be caused by
inappropriate waste handling of this product.
FCC 47 CFR Part15 §15.107 and §15.109
EN 301 489-1 V1.9.2 Radio and ancillary equipment for portable use (portable equipment); EUT
Operating frequency range: 2.4 – 2.4835 GHz
EN 300 328 V1.8.1 Electromagnetic compatibility and Radio spectrum Matters (ERM);
Wideband transmission systems; Data transmission equipment operating
in the 2,4 GHz ISM band and using wide band modulation techniques;
Harmonized EN covering the essential requirements of article 3.2 of the
R&TTE Directive
EN 301 489-17 V2.2.1 Electromagnetic compatibility and Radio Spectrum Matters (ERM)
IEC 62133 and UN38.3 Battery certification and transport test
Standards to which conformity is declared, where relevant, are as follows:
IEC/EN 61010-1:2012 Safety requirements for electrical equipment for measurement, control,
and laboratory use. General requirements.
EN61326-1:2013 Electromagnetic compatibility- generic emission standard electrical
equipment for measurement, control, and laboratory use.
Signed:
Dr. Thomas Sahiri
Managing Director
Implen GmbH

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Contents
1. NANOPHOTOMETER®AT A GLANCE.......................................................................8
NANOPHOTOMETER®OVERVIEW ..............................................................................................8
NANOPHOTOMETER®NP80 ................................................................................................................9
NANOPHOTOMETER®N60.................................................................................................................10
NANOPHOTOMETER®N50.................................................................................................................11
NANOPHOTOMETER®C40.................................................................................................................12
INSTRUMENT REAR PANEL (NP80/N60/N50/C40)..............................................................................13
INSTRUMENT BOTTOM VIEW (NP80/N60/N50/C40)............................................................................13
ACCESSORIES.......................................................................................................................14
STANDARD ACCESSORIES.................................................................................................................14
OPTIONAL ACCESSORIES..................................................................................................................14
CONNECTIVITY.......................................................................................................................18
NANOPHOTOMETER®SPECIFICATIONS ....................................................................................19
2. GETTING STARTED..............................................................................................21
SPECTROPHOTOMETER INSTALLATION ....................................................................................21
SAFETY INFORMATION ......................................................................................................................21
UNPACKING AND POSITIONING ..........................................................................................................22
SOFTWARE INSTALLATION......................................................................................................23
NPOS OVERVIEW.............................................................................................................................23
REQUIREMENTS AND COMPATIBILITY.................................................................................................23
INSTALLING SOFTWARE ON COMPUTER..............................................................................................24
INSTALLING NANOPHOTOMETER®APP ON TABLET OR SMARTPHONE..................................................25
FIRST STEPS AND CONFIGURATION WIZARD........................................................................................26
PRINTER INSTALLATION ....................................................................................................................26
3. NANOPHOTOMETER®BASICS..............................................................................27
APPLICATIONS OVERVIEW......................................................................................................27
ICONS...................................................................................................................................29
BUTTONS..............................................................................................................................30
SIDE TAB BAR ..................................................................................................................................31
DATA PROCESSING DIALOGS.............................................................................................................33
BASIC OPERATION.................................................................................................................36
NANOVOLUME MEASUREMENT BASICS (N50/N60/NP80)...................................................................36
CUVETTE MEASUREMENT BASICS (C40/NP80)..................................................................................37
SUBMICROLITER CELL MEASUREMENTS BASICS (C40).......................................................................39
SAMPLE HANDLING TIPS...................................................................................................................40
DATA TRANSFER..............................................................................................................................42

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BATTERY OPERATION ............................................................................................................43
4. NANOPHOTOMETER®APPLICATIONS ...................................................................44
NUCLEIC ACIDS ..................................................................................................................44
METHOD OVERVIEW..........................................................................................................................44
MEASUREMENT PROTOCOL...............................................................................................................44
CALCULATIONS ................................................................................................................................48
PROTEIN UV ......................................................................................................................53
METHOD OVERVIEW..........................................................................................................................53
MEASUREMENT PROTOCOL...............................................................................................................54
CALCULATIONS ................................................................................................................................57
PROTEIN ASSAYS ...............................................................................................................61
METHOD OVERVIEW..........................................................................................................................61
MEASUREMENT PROTOCOL...............................................................................................................62
CALCULATIONS ................................................................................................................................66
KINETICS ..........................................................................................................................66
METHOD OVERVIEW..........................................................................................................................66
MEASUREMENT PROTOCOL...............................................................................................................66
CALCULATIONS ................................................................................................................................68
OD600 ..........................................................................................................................68
METHOD OVERVIEW..........................................................................................................................68
MEASUREMENT PROTOCOL...............................................................................................................70
CALCULATIONS ................................................................................................................................72
MORE APPS .......................................................................................................................73
MORE APPS:WAVELENGTH ................................................................................................73
METHOD OVERVIEW..........................................................................................................................73
MEASUREMENT PROTOCOL...............................................................................................................73
CALCULATIONS ................................................................................................................................76
MORE APPS:WAVESCAN ...................................................................................................76
METHOD OVERVIEW..........................................................................................................................76
MEASUREMENT PROTOCOL...............................................................................................................76
CALCULATIONS ................................................................................................................................79
MORE APPS:ABSORBANCE RATIO .....................................................................................79
METHOD OVERVIEW..........................................................................................................................79
MEASUREMENT PROTOCOL...............................................................................................................79
CALCULATIONS ................................................................................................................................82
MORE APPS:CONCENTRATION ...........................................................................................82
METHOD OVERVIEW..........................................................................................................................82
MEASUREMENTS PROTOCOL.............................................................................................................82
CALCULATIONS ................................................................................................................................85

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MORE APPS:STANDARD CURVE ........................................................................................86
METHOD OVERVIEW..........................................................................................................................86
MEASUREMENTS PROTOCOL.............................................................................................................86
CALCULATIONS ................................................................................................................................89
CUSTOM APPS ......................................................................................................................90
STORED RESULTS ..............................................................................................................90
STORED METHODS .............................................................................................................91
5. PREFERENCES....................................................................................................92
GENERAL..............................................................................................................................92
DATE AND TIME................................................................................................................................92
DISPLAY ..........................................................................................................................................92
ABOUT.............................................................................................................................................93
LANGUAGE.......................................................................................................................................93
ILLUMINATION SAMPLE WINDOW .......................................................................................................94
ENABLE NANOVOLUME.....................................................................................................................94
DYES 94
WARNING MESSAGES ............................................................................................................95
BLANK CONTROL .............................................................................................................................95
SAMPLE QUALITY CONTROL..............................................................................................................95
NETWORK.............................................................................................................................96
WIFI...................................................................................................................................97
NETWORK PRINTER ...............................................................................................................97
REPORT CONFIGURATION.......................................................................................................98
6. TROUBLESHOOTING............................................................................................99
SELF-CALIBRATION TEST ........................................................................................................99
7. ASSISTANCE.....................................................................................................100
SUPPORT............................................................................................................................100
REPORT PROBLEM ..............................................................................................................100
SOFTWARE MAINTENANCE ...................................................................................................101
RESET ...........................................................................................................................................101
SOFTWARE UPDATE........................................................................................................................101
CREATE LOG FILE ..........................................................................................................................101
INSTALL PRINTER DRIVER...............................................................................................................102
LEGAL ...........................................................................................................................................102
TRADEMARKS......................................................................................................................102
CONTACT IMPLEN................................................................................................................103

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8. MAINTENANCE..................................................................................................104
MAINTENANCE FREE TECHNOLOGY.......................................................................................104
REPLACEMENT PARTS .........................................................................................................104
CLEANING AND GENERAL CARE ...........................................................................................105
MESSAGES .........................................................................................................................105
IMPORTANT WARNING MESSAGES:..................................................................................................105
IMPORTANT ALERT MESSAGES: ......................................................................................................107
9. WARRANTY.......................................................................................................109
10. APPENDIX 1- INSTALACE SPEKTROFOTOMETRU .................................................110
BEZPEČNOSTNÍ INFORMACE.................................................................................................110
VYBALENÍ A UMÍSTĚNÍ ..........................................................................................................111
11. APPENDIX 2-INSTALLATIE SPECTROFOTOMETER ..............................................112
VEILIGHEIDSINFORMATIE ......................................................................................................112
UITPAKKEN EN PLAATSEN ....................................................................................................113
12. APPENDIX 3-INSTALLATION DU SPECTROPHOTOMÈTRE.....................................114
INFORMATIONS DE SÉCURITÉ ................................................................................................114
DÉBALLAGE ET POSITIONNEMENT .........................................................................................115
13. APPENDIX 4–INSTALLATION SPECTROPHOTOMETER.........................................117
SICHERHEITSINFORMATIONEN...............................................................................................117
AUSPACKEN UND PLATZIEREN..............................................................................................117
14.APPENDIX 5–INSTALLAZIONE DELLO SPETTROFOTOMETRO ..............................119
INFORMAZIONI DI SICUREZZA.................................................................................................119
DISIMBALLAGGIO E COLLOCAZIONE.......................................................................................120
15. APPENDIX 6–INSTALACJA SPEKTROFOTOMETRU..............................................121
INFORMACJE DOTYCZĄCE BEZPIECZEŃSTWA..........................................................................121
ROZPAKOWYWANIE I UMIEJSCOWIENIE ..................................................................................122
16. APPENDIX 7–INSTALACIÓN DEL ESPECTROFOTÓMETRO....................................124
INFORMACIÓN DE SEGURIDAD ...............................................................................................124
DESEMBALAJE E INSTALACIÓN .............................................................................................125
17. APPENDIX 8–SPEKTROFOTOMETERINSTALLATION............................................126

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SÄKERHETSINFORMATION ....................................................................................................126
UPPACKNING OCH PLACERING ..............................................................................................127
18. APPENDIX 9–INSTALAÇÃO DE ESPECTROFOTÓMETRO ......................................128
INFORMAÇÕES DE SEGURANÇA ............................................................................................128
DESEMBALAMENTO E POSICIONAMENTO ...............................................................................129
19. ALPHABETICAL APPENDIX.................................................................................131

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1.NANOPHOTOMETER®AT A GLANCE
NANOPHOTOMETER®OVERVIEW
The Implen NanoPhotometer®spectrophotometer is a mobile, simple to use UV/Visible instrument
with a CCD array detector with options ranging from nanovolume to standard cuvette and all-in-one
solutions. The N50, N60, and NP80 spectrophotometers measure sample volumes ranging from 0.3 -
2 µl. The NP80 model also has the capability to measure samples using a standard cuvette. The C40
is a mobile standard cuvette solution with the option to upgrade to accommodate nanovolume
measurements.
The NanoPhotometer®runs on a Linux-based operating system (NPOS) that is designed for the use
of pre-programmed and custom applications with a high degree of flexibility and processing power.
Sample Compression Technology™provides easy sample handling which is independent of surface
tension. This technology squeezes the sample between two quartz surfaces allowing for unmatched
precision and accuracy without the need for dilutions. Combined with our True Path Technology™ the
system offers lifetime accuracy and precision without the need for maintenance or recalibration.
Note: It is recommended to use a properly calibrated pipette with high-quality tips to ensure delivery
of appropriate sample volumes for nanovolume sample applications.
Sample Control™ is a quality control technology that identifies air bubbles, sample impurities,
turbidity, lint residues and potential contaminations. Sample Control™ monitors handling
characteristics and sample quality in real time to ensure that the measured concentrations are
reproducible and most precise.
Blank Control™ gives a warning message for blanks with high background. High background
absorbance can be caused by a contaminated blank, blank buffer or by residues from previous users.
Insuficient blank readings are the main cause for inaccurate measurements. Blank Control™ will
protect the user from wasting time and precious sample on inaccurate readings caused by high
background blanks or inappropriate cleaning. For more detailed information see also Technical Note
#1 Blank Control™.

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NANOPHOTOMETER®NP80
Item Number
Touchscreen
Battery Pack
NP80
-
-
NP80-Touch
+
-
NP80-Mobile
+
+

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NANOPHOTOMETER®N60
Item Number
Touchscreen
Battery Pack
N60
-
-
N60-Touch
+
-
N60-Mobile
+
+