Neogen corporation 4700 User manual

Designed for use with Neogen’s Veratox®, BioKits and

GeneQuence®microwell test kits

The 4700 Reader is a compact, economical, stand-alone microstrip reader. Its streamlined design

offers a touch screen interface, superb optics, onboard curve-ﬁtting software, and built-in printer

to meet the requirements of modern laboratories.

4700 Reader User’s Manual

2User’s Manual •Neogen 4700 Reader

3Questions? Call Neogen at 800-234-5333 or 517-372-9200

Table of contents

Section 1: Installation......................................................................................................... 4

1.1 Unpacking and installation....................................................................................... 4

1.2 Load paper................................................................................................................. 4

Section 2: Set up ..................................................................................................................... 5

2.1 Starting the reader.................................................................................................... 5

2.2 Using the touch screen............................................................................................. 5

2.3 The home screen....................................................................................................... 5

2.4 Utility menu ............................................................................................................... 5

Section 3: Running the test .................................................................................................... 6

Section 4: Reading and calculating results........................................................................... 6

Section 5: Reading and calculating GeneQuence results..................................................... 8

Section 6: Maintenance .......................................................................................................... 8

Section 7: Settings ............................................................................................................. 8

7.1 Printer setup ......................................................................................................... 8

7.2 Adjust date and time............................................................................................. 9

7.3 Lamp control......................................................................................................... 9

7.4 Laboratory name................................................................................................... 9

7.5 Strip format........................................................................................................... 9

7.6 Sound settings .................................................................................................... 10

7.7 External output.................................................................................................... 10

Section 8: Cloning tests.................................................................................................... 10

Section 9: Editing preprogrammed tests.......................................................................... 10

Section 10: Programming new test parameters .............................................................. 12

Section 11: Deleting tests................................................................................................. 12

Section 12: Restoring tests .............................................................................................. 12

Section 13: Modes............................................................................................................ 13

Section 14: Troubleshooting............................................................................................. 13

4User’s Manual •Neogen 4700 Reader

Section 1. Installation

1.1 Unpacking and installation

1. Carefully unpack the instrument, removing it from its plastic bag. Report any damage

to the freight carrier at once. NOTE: Retain the original packing material for future

use in the event that the instrument is shipped to another location or returned for

service.

2. Place the instrument on a ﬂat working surface capable of safely supporting the

weight of the instrument (approximately 5 lbs. (2.3 kg)). A clearance of at least 3

inches around the instrument is required to ensure optimal ventilation. The instru-

ment should be operated within an ambient temperature range of 18–35°C and at

humidity of less than 85%.

3. Ensure the power switch on the back of the unit is in the off (down) position before

connecting the power cord to the power supply.

4. With the power switch in the Off (down) position, insert the DC connector attached

to the end of the power supply module cable to the instrument. Insert the mating end

of the AC power cord to the inlet of the power supply module, and plug the other end

of the AC power cord into an AC outlet. Use only the power cord and supply module

speciﬁed for this product and certiﬁed for the country of use.

• For 110–120 V used in the United States, use the supplied UL listed cord set

consisting of an 18 AWG, Type SPT-1 two conductor cord maximum 3 meters

(10 feet) in length, rated 7 A, 125 V, with a polarized parallel blade type attach-

ment plug.

• For 220–240 V used inside the United States, use a UL listed cord as above,

except rated 250 V.

• For other locations, use the power cord certiﬁed for the country of use.

1.2 Load paper

1. Locate the roll of thermal printer paper

(Neogen item 9319).

2. Lift the printer paper compartment lid

and open the printer compartment.

3. Place the paper roll in the well so the

leading edge of the paper feeds toward

the front of the printer from the bottom

of the paper roll.

4. Pull up at least 1 inch of paper and then press the compartment cover down until it

snaps closed.

5Questions? Call Neogen at 800-234-5333 or 517-372-9200

Section 2. Set up

2.1 Starting the reader

1. Turn the power switch, which is located on the power supply module, On. The printer

will print several lines. If the reader does not print, then the internal printer is disabled.

To enable printing, go to Settings to turn printer On.

2. Refer to the Veratox Software for Windows instruction manual version 3.3 or newer

for setting up the USB connection.

2.2 Using the touch screen

The touch screen can be manipulated using the stylus or by touch. Icons also can be

selected by tapping the stylus on the screen.

2.3 The home screen

The home screen has several icons:

1. Run test: Contains preprogrammed tests

used to read samples.

2. Manage tests: Contains options for creat-

ing, editing, restoring and deleting tests.

3. Settings: Contains time and date, printer

and strip format settings.

4. Utilities: Contains options to erase all tests, change access levels, adjust ﬁlter volt-

ages and recall calibration.

5. Lamp: Touching this icon will turn the lamp on or off.

6. FF: Touching this icon will feed paper through the slot.

2.4 Utility menu

1. Change access level: To change the

access level, contact Neogen Techni-

cal Services for a passcode.

2. Show version details: Touching this

icon will display the current ﬁrmware

version, model, serial number, build

date and current time.

3. Erase all tests: Touch this icon to erase all tests. The reader will prompt the user to

“Please conﬁrm: Erase all tests?” CAUTION: Selecting OK will delete all tests.

4. Recall calibration: Allows user to restore the original factory settings, including

calibration.

6User’s Manual •Neogen 4700 Reader

5. Print unit settings: Prints all information about an individual instrument’s calibra-

tion settings and other information.

6. Filter voltages: Displays real-time ﬁlter wheel voltages with an option to print them.

Section 3. Running the test

1. Run the assay according to the Directions for use supplied with the test kit. Results

should be processed by the reader within 20 minutes of completion of the test to

ensure accuracy.

Section 4. Reading and calculating results

1. Wipe bottom of microwells clean before reading.

2. Insert wells into the far right slot of the well holder with the zero in the top position.

Place any additional strips in positions B and C.

3. Position the carrier to the left so strip A is in the center of the track.

4. Use the Run Test key to access the user test menu. The instrument will display

the 33 preprogrammed tests. Arrow up “^” or down “v” to scroll, and page forward

using“>>” or page back using“<<” to move through the list. Or select “By #,” and

enter the test number and press Enter.

5. Conﬁrm the test selection or press No to select a different test.

6. To print the test menu select the Manage Tests icon then Print Test List.

7. One curve may be used to calculate up to 24 wells, but under no circumstances

should a curve from one run be used to calculate samples that have not been run at

the same time as the standard curve.

8. After selecting a test there will be three options — accept the test selected, limit the

number of wells to read, or quit the test.

9. If the correlation coefﬁcient is < 0.980, the message “Invalid! r < 0.98” will be

printed at the end of the run. Results should be considered invalid if this message

appears.

7Questions? Call Neogen at 800-234-5333 or 517-372-9200

Neogen Menu

Test name Control values Unit of

measure

1 Aﬂatoxin 0.0 5.0 15.0 50.0 — — ppb

2 Aﬂatoxin HS 0.0 1.0 2.0 4.0 8.0 — ppb

3 Aﬂatoxin M1 0.0 5.0 15.0 30.0 60.0 100 ppt

4 Aﬂatoxin HS MAX 0.0 1.0 3.0 5.0 10.0 — ppb

5 DON 0.0 0.5 1.0 2.0 6.0 — ppm

6 DON HS 0.0 25.0 50.0 100.0 250.0 — ppb

7 DON NE 0.0 0.25 0.5 1.0 2.0 — ppm

8 Fumonisin 5/10 0.0 0.25 1.0 3.0 6.0 — ppm

9 Fumonisin 10/10 0.0 1.0 2.0 4.0 6.0 — ppm

10 Fumonisin HS 0.0 50.0 100.0 300.0 600.0 — ppb

11 Zearalenone 0.0 25.0 75.0 150.0 500.0 — ppb

12 T-2/HT-2 Toxins 0.0 25.0 50.0 100.0 250.0 — ppb

13 Ochratoxin 0.0 2.0 5.0 10.0 25.0 — ppb

14 Histamine 0.0 2.5 10.0 20.0 50.0 — ppm

15 Peanut Allergen 0.0 2.5 5.0 10.0 25.0 — ppm

16 Almond Allergen 0.0 2.5 5.0 10.0 25.0 — ppm

17 Egg Allergen 0.0 2.5 5.0 10.0 25.0 — ppm

18 Total Milk Allergen 0.0 2.5 5.0 10.0 25.0 — ppm

19 Casein Allergen 0.0 2.5 5.0 10.0 15.0 — ppm

20 Gliadin 0.0 5.0 10.0 20.0 50.0 — ppm

21 Soy Allergen 0.0 2.5 5.0 10.0 25.0 — ppm

22 Hazelnut Allergen 0.0 2.5 5.0 10.0 25.0 — ppm

23 Giladin (2.5–40) 0.0 2.5 5.0 10.0 20.0 40.0 ppm

24 Gliadin (5–40) 0.0 5.0 10.0 20.0 40.0 — ppm

25 Coconut 0.0 1.0 2.5 5.0 10.0 25.0 ppm

26 Mustard Allergen 0.0 2.5 5.0 10.0 25.0 — ppm

27 Crustacea Allergen 0.0 2.5 5.0 10.0 25.0 — ppm

28 Veratox Sesame 0.0 2.5 5 10 25 — ppm

29 BioKits Walnut 0.0 2.4 6.0 12.0 60.0 120.0 ppm

30 GeneQuence See Section 5

31 Absorbance 450 nm

32 Absorbance 650 nm

33 Dri Dye

NOTE: Neogen’s Veratox format tests have been preprogrammed for convenience. To enter new

test parameters or change an existing test, see Sections 8 and 9.

8User’s Manual •Neogen 4700 Reader

Section 5. Reading and calculating GeneQuence results

1. From the Home screen, select Run Test. Select GeneQuence and conﬁrm the se-

lection.

2. Press Accept. The reader then will prompt the user to “Set the carrier to the ﬁrst

strip, then press OK.” Once the carrier is set, press OK.

3. If NC > 0.15, the test will be invalid.

4. If PC < 1.0, the test will be invalid.

Section 6. Maintenance

It is important to follow the installation instructions carefully, using only a suitable power

supply, surge protector and placing the instrument with the proper clearance for good air

circulation around it. Excessive vibration should be avoided. During shipments, use the

original packing material or other suitable protective foam.

The Neogen 4700 Reader essentially is a maintenance-free instrument. To ensure maxi-

mum trouble-free operation, the instrument need only be kept dry. Although the ﬁlters are

well sealed, their life may be decreased if the instrument is maintained in a very humid

environment (greater than 80% humidity). Using the instrument in an air-conditioned

room is recommended for humid climates. Extreme temperature shock also is harmful to

the ﬁlters. Maximum changes of 5°C per minute are recommended especially at the lower

limits of -50°C, where permanent damage may occur. The reader is designed for use at

20°C and performs according to speciﬁcations in the range of 10–50°C.

Cleaning should be done only when obviously necessary. Use a dry cloth or duster to

remove dust and dirt. Use a slightly damp, soft cloth to clean up spills. Water or 70%

isopropanol may be used to dampen the cloth. Use of other chemicals or abrasive scrub-

bing may damage the cover.

Section 7. Settings

7.1 Printer setup

For paper loading instructions, see Section

1.2. If the printer does not print several lines

upon turning on the Neogen 4700 Reader,

the printer is disabled. To enable the printer,

go to Settings, followed by Printer Setup.

The touch screen will present an On and Off

option as well as the current status of the

printer. To enable the printer, touch On using

the provided stylus, followed by Save.

9Questions? Call Neogen at 800-234-5333 or 517-372-9200

To print a list of test options, touch the Manage Tests icon. Press the Print Test List

icon. The reader will print a list of the programmed tests along with their assigned test

number.

7.2 Adjust date and time

To set the correct time and date, touch the Settings icon on the home screen. Once in

Settings, touch the Adjust Date and Tme icon and hit Select. To edit the hour, minute

and seconds ﬁelds, touch the stylus to the desired ﬁeld. The screen will show the selected

ﬁeld. Touch the correct number and hit Enter. To erase an existing number use the back-

space icon (<–) or the Clear icon. Once the desired number is in the ﬁeld, press Enter.

Do the same to set the month, date and year, which is located below the time settings in

the Set Time window. Once back in the Set Time window, press Save to allow changes

to take effect.

The Set Time window also allows users to switch the format of date between U.S. and

European formats. To change the style, touch Date Style in the Set Time screen. The

date will be displayed in the desired format next to the Date Style icon. Press Save for

the changes to take effect.

The Set Time window also has a Set Both option. This allows users to input the month,

day, and year in a MMDDYY format. After entering the numbers in the Set Both screen,

press Enter followed by Save on the Set Time screen.

The Edit icon in the Set Time screen also allows users to change time and date settings.

7.3 Lamp control

The length of time the lamp will remain on before it turns off and the minimum warmup

time can be adjusted by touching the Settings icon, followed by the Lamp Settings.

Touch the desired ﬁelds to edit the time in seconds. Touch Save to implement the new

settings.

7.4 Laboratory name

Users can change the name of the laboratory, which is printed upon startup. From the

Home screen, touch Settings, followed by Laboratory name.

Press Edit to change the name. Once ﬁnished, touch Enter. To implement the name,

press Save. To exit the menu, press Cancel.

7.5 Strip format

To change the strip format between 8 wells and 12 wells, touch Settings, followed by

Strip Format. Touch either 8 well or 12 well, followed by Save to implement the

change.

10 User’s Manual •Neogen 4700 Reader

7.6 Sound settings

To access the sound settings, touch Settings, followed by Sound Settings. From here,

users can turn on or off the startup sounds, icon press and release sounds, and other

sounds. Users also can change the volume by touching the appropriate ﬁeld. Press Save

to implement changes.

7.7 External output

Neogen’s Veratox software can be used

to store and transmit data from the Neo-

gen 4700 Reader.

To conﬁgure external output settings,

touch the Settings icon, followed by Ex-

ternal Output. Press Select. In the Ex-

ternal Output menu, select On. Press

Save to implement the changes.

Connect the smaller end (Type B) of USB cable to the type B port on the lower left back-

side of the reader as shown above.

Turn on the computer. Connect the larger end (Type A) of the USB port to the USB port

on the computer.

For instructions on using Veratox software, see the software manual.

Section 8. Cloning tests

The clone function allows users to duplicate an existing test and assigns the next available

test slot number, which allows the test to be stored.

1. From the Home screen, touch the Manage Tests icon.

2. Touch the Clone Test icon.

3. Select the test to be cloned from the list and press Select. The reader will ask the

selection to be conﬁrmed. Touch Yes to continue or No to return to the list.

4. The reader will indicate that “Test # was cloned into Test #.”

5. To edit the name of the cloned test and to alter the control values, return to the Man-

age Tests screen and select the Edit icon. See Section 9 for additional instructions.

Section 9. Editing preprogrammed tests

To change the settings on an already established test, follow the directions below:

1. From the Home screen, touch the Manage Tests icon.

2. Touch the Edit icon.

11Questions? Call Neogen at 800-234-5333 or 517-372-9200

3. Select which test to edit by touching the test name and then Select. The reader will

ask for the selection to be conﬁrmed. Touch Yes to continue or No to return to the

selection screen.

4. On the ﬁrst screen, the reader will display the Test Deﬁnition screen where the

test name, mode, ﬁlters and blanks can be edited. To edit the ﬁelds, either touch

the desired ﬁeld with the stylus or use the Edit icon. Once ﬁnished editing the ﬁelds,

touch the double arrow icon to move to the next page. The reader will save any

changes that have been made.

a. Enter the name of the test (e.g., Aﬂatoxin test). Touch Enter to submit the name.

b. Touch the Mode ﬁeld. Select the desired test mode by touching the appropriate

ﬁeld and then hitting Select. (For more information on the different modes, see

Section 13 ).

c. Select the ﬁlters by touching the Primary Filter and the Differential Filter

icons and choosing the appropriate number. Touch Select to implement.

d. Choose whether blanks will be used and the number of replicates by touching the

Blank ﬁeld. After choosing the desired settings, touch Select.

On the second screen:

a. Standards: Select the number of standards for the new assay.

b. # Std. replicates: If the assay will run standards in duplicate, select “2”. Other-

wise, the default “1” will be used.

c. Axes: The default will set absorbance on the Y axis and the concentration on the

X axis.

d. Normal control, abnormal control and other control: These functions are

parameters set up for control tolerances. It is recommended these functions re-

main disabled.

On the third screen:

a. Interpretation mode: This allows the user to input acceptable ranges for opti-

cal density. It is recommended these ﬁelds are left blank.

b. Units: Select the appropriate unit of measure (ppm or ppb) for the assay.

c. Decimals: Select the desired amount of ﬁgures to display after the decimal.

d. # sample replicates: Select the number of sample replicates used in the assay.

The default assumes running only one sample per microwell.

12 User’s Manual •Neogen 4700 Reader

5. If using the test setting only once, touch Run to activate the test. To save the setting

to the list of preprogrammed tests, touch the Save icon (a window will pop up indi-

cating the test has been saved under a new test number). To Print the test settings,

touch the Print icon.

6. To return to the Home screen, touch Exit. NOTE: To save test settings, make sure

to touch Save before exiting.

Section 10. Programming new test parameters

1. For ease of use, Neogen recommends users clone tests rather than programming

new parameters. For details on cloning tests, see Section 8.

Section 11. Deleting tests

1. From the Home screen, touch the Manage tests icon.

2. Select Delete test.

3. Select a test from the list to delete by touching the desired test name.

4. The reader will ask the selection be conﬁrmed. Touch Yes to continue or No to return

to the list.

5. The reader will indicate that “Test # has been deleted.”

Section 12. Restore tests

Users can choose to restore one of the preprogrammed tests or to restore all of the

preprogrammed tests if they have been deleted. NOTE: If a user-programmed test exists

in place of preprogrammed test, the restore function will overwrite the user programmed

test.

1. From the Home screen, touch the Manage tests icon.

2. Select Restore tests. A message will ap-

pear reading, “Warning! Preprogrammed

tests will overwrite user tests with the

same number. Continue?” Touch Yes to

continue or No to return to the Manage

Tests screen.

3. The user then will be prompted to, “Restore just one test?” To choose, touch Yes.

To restore all tests, press No. If No is chosen, the reader then will restore all prepro-

grammed tests.

4. If only restoring one test, the reader will ask for the number of the test to be restored.

Input the number of the test to restore and press Enter.

13Questions? Call Neogen at 800-234-5333 or 517-372-9200

Section 13. Modes

For more detail on using each mode, please reference the additional Neogen 4700 Reader

manual that came with the reader.

1. Absorbance: This mode reads and prints the monochromatic or bichromatic

differential absorbance at the user-selected wavelengths. Blanks are optional. Most

assays require a mode other than absorbance mode as no calculations are made in

this mode —only absorbance values are reported.

2. Factor: In this mode, the endpoint absorbance readings will be multiplied by a user-

entered factor to calculate a result.

3. Single standard: This mode reads a calibrator and calculates concentrations based

on a single point standard curve passing through the point (0,0). A blank is required

to determine the (0,0) point. A factor (equal to the concentration of calibrator plus

the absorbance of calibrator) is generated in this mode, and then multiplied by sub-

sequent absorbance readings to determine concentrations.

4. Point-to-point: The microstrip reader accepts a number of calibrators and calcu-

lates concentrations based on the point-to-point calibration curve. Calibrator materi-

als of known concentrations are used to calibrate the reader so concentration of

unknown samples may be calculated.

5. Regression: In this mode, the instrument accepts a number of calibrators and cal-

culates concentration values based on a best-ﬁt curve (linear regression).

6. Cubic spline: Cubic spline mode accepts a number of calibrators and calculates

concentrations based on the cubic spline (constrained) calibration curve. Calibrator

material of known concentrations are used to calibrate the reader so concentrations

of unknown samples are calculated from the generated curve. The resulting curve is

a smooth curve connecting the calibrator points, which may be entered in ascending

or descending order of absorbance.

Section 14. Troubleshooting

For error message deﬁnitions and common questions, see the additional Neogen 4700

Reader owners manual that came with the reader.

14 User’s Manual •Neogen 4700 Reader

_________________________________________________________

15Questions? Call Neogen at 800-234-5333 or 517-372-9200

_________________________________________________________

©Neogen Corporation, 2019. Neogen, GeneQuence, and Veratox are registered trademarks of Neogen Corporation, Lansing, MI, 48912.

Neogen’s 4700 Reader microwell reader is manufactured by Awareness Technology, Inc., Palm City, FL. All other brand and product names

are trademarks or registered trademarks of their respective companies.

FD327-0519

9639C

North America

Neogen Headquarters

800/234-5333 (USA/Canada)

[email protected]

foodsafety.neogen.com

Europe, Middle East and Africa

Neogen Europe

+ 44 (0) 1292 525 600

[email protected]

foodsafety.neogen.com/uk/

Mexico

Neogen Latinoamerica

+52 (55) 5254-8235

[email protected]

foodsafety.neogen.com/sp/

Brazil

Neogen do Brasil

+55 19 3935.3727

[email protected]

foodsafety.neogen.com/pt/

China

Neogen Bio-Scientiﬁc Technology

+86 21 6271 7013

[email protected]

www.neogenchina.com.cn

India

Neogen Food and Animal Security

+91 484 2306598, 2301582

[email protected]

www.neogenindia.com

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