Thermo Scientific CTS Xenon User manual

For Research Use or Manufacturing of Cell, Gene, or Tissue- Based
Products. CAUTION: Not intended for direct administration into
humans or animals.
CTS™Xenon™Electroporation System
USER GUIDE
For electroporation of primary and stem cells for cell therapy
development and manufacturing
Catalog Number A52727
Publication Number MAN0025488
Revision A.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0025488
Revision Date Description
A.0 11 November 2021 New user guide for CTS™Xenon™Electroporation Instrument.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2021 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER 1 Product information .................................................. 6
Product description ............................................................. 6
Product contents ................................................................ 7
CTS™Xenon™system contents ............................................... 7
CTS™Xenon™buer kit contents ............................................. 7
System components ............................................................. 8
CTS™Xenon™Electroporation Instrument ...................................... 8
SingleShot electroporation chamber ......................................... 10
MultiShot electroporation cartridge .......................................... 11
Xenon™buer kits ......................................................... 12
Upon receiving the instrument ................................................... 12
Guidelines for unpacking and set up of the instrument ......................... 12
Required materials not provided ............................................. 13
User interface overview ..................................................... 14
Touchscreen controls ...................................................... 15
First time instrument setup ...................................................... 17
Connect the instrument to the Internet ....................................... 17
Create a user profile on the instrument ....................................... 21
Manage user profiles ....................................................... 22
About the Thermo Fisher™Connect Platform ...................................... 23
Create a Connect account .................................................. 23
Link the instrument to Connect (Administrator only) ............................ 24
Connect the instrument to the Internet ....................................... 26
Create a PIN number ....................................................... 26
Generate a link code from the instrument ..................................... 26
Set up a new Administrator ................................................. 26
Add an instrument to your Connect account .................................. 27
CTS™Xenon™Electroporation System User Guide 3

■CHAPTER 2 Methods ............................................................. 29
Electroporation protocol options ................................................. 29
Electroporation parameters ................................................. 29
Create/Edit SingleShot protocols ................................................. 30
Create SingleShot Protocol ................................................. 30
Edit SingleShot Protocol .................................................... 31
Create/Edit MultiShot protocols .................................................. 33
Create MultiShot Protocol .................................................. 33
Edit MultiShot Protocol ..................................................... 34
Convert a SingleShot protocol to a MultiShot protocol ......................... 34
Save a Protocol ................................................................ 35
Import protocols ............................................................... 35
Manage protocols .............................................................. 35
General guidelines ............................................................. 36
Guidelines for electroporation payloads ...................................... 36
Controls .................................................................. 37
Required materials ............................................................. 37
Prepare cells .................................................................. 38
Activate T cells ............................................................ 38
Prepare cells for electroporation ............................................. 38
Perform electroporation ......................................................... 40
Run SingleShot protocol .................................................... 40
Run MultiShot protocol ..................................................... 42
Post-electroporation procedure .............................................. 46
Maintenance .................................................................. 47
Cleaning and maintenance .................................................. 47
Replace the fuses .......................................................... 48
Upgrade the system firmware ............................................... 48
Self Verification test ........................................................ 51
Restore factory settings (Administrator profile only)............................ 51
Repackaging the instrument ................................................ 52
■APPENDIX A Troubleshooting .................................................... 53
Troubleshooting ................................................................ 53
Remove jammed electroporation chamber ........................................ 55
Remove the plastic cover ................................................... 55
Error codes ................................................................... 57
■APPENDIX B Optimization ........................................................ 59
Optimization protocol ........................................................... 59
Contents
4CTS™Xenon™Electroporation System User Guide

■APPENDIX C Recommended instrument settings .............................. 60
About Instrument .............................................................. 60
Recommended instrument settings .............................................. 60
Instrument settings (Administrator only) ...................................... 61
Maintenance & services ......................................................... 65
Run History ................................................................... 66
■APPENDIX D Specifications ...................................................... 68
Product specifications .......................................................... 68
■APPENDIX E Related products ................................................... 69
Accessory products ............................................................ 69
Additional products ........................................................ 69
■APPENDIX F Safety ............................................................... 71
Safety information .............................................................. 71
Safety compliance ............................................................. 72
Informational symbols .......................................................... 72
Symboles d’information ......................................................... 73
Environmental requirements ..................................................... 74
Electromagnetic compatibility (EMC) standards .................................... 75
Class A notice ............................................................. 75
EMC compliance .......................................................... 75
Electrical safety ................................................................ 76
Chemical safety ................................................................ 77
Biological hazard safety ......................................................... 78
■APPENDIX G Documentation and support ...................................... 79
Customer and technical support ................................................. 79
Limited product warranty ........................................................ 79
Contents
CTS™Xenon™Electroporation System User Guide 5

Product information
Product description
The CTS™Xenon™Electroporation System is a novel, benchtop device consisting of the CTS™Xenon™
Electroporation Instrument, single-use consumables for performing transfection of cells, and specialized
buers.
The CTS™Xenon™Electroporation Instrument eciently delivers gene editing payloads (DNA, RNA
and proteins) into all mammalian cell types including primary and stem cells with a high cell survival
rate. Protocols are optimized for ease of use and simplicity, and conditions can be optimized for your
payload and cell type. See “System components” on page 8 for details on various parts of the
system.
Two types of consumable are used for performing transfection.
• The SingleShot format uses an electroporation chamber to process 2 × 107to 1 × 108cells in a
1 mL sample volume. See “SingleShot electroporation chamber” on page 10.
• The MultiShot format uses a cartridge and sample bags to process 1 × 108to 2.5 × 109cells in a
5–25 mL sample volume. See “MultiShot electroporation cartridge” on page 11
The system includes proprietary buers that are compatible with various cell types including primary
lymphocytes and stem cells. See “Xenon™buer kits” on page 12
•The CTS™Xenon™Electroporation Buer is compatible with a wide variety of mammalian cell
types, eliminating the need to determine an optimal buer for each cell type.
•The CTS™Xenon™Genome Editing Buer is designed to improve performance with gene editing
specific payloads (Talen, Cas9) in mammalian primary and stem cells.
1
6CTS™Xenon™Electroporation System User Guide

Product contents
CTS™Xenon™system contents
The contents of the CTS™Xenon™Electroporation System are listed in the following table. The system
is shipped at room temperature.
See page 8 for specifications and description of the CTS™Xenon™Electroporation Instrument, and
page 29 to set up the instrument.
Product Quantity
CTS™Xenon™Electroporation Instrument 1
Region specific power cord
(for US/Canada/Taiwan/Japan, Europe, or UK)
1
Cat 5 Ethernet cable 1
USB memory device (containing user guide) 1
Quick reference 1
Install guide 1
Bubble level 1
CTS™Xenon™buer kit contents
The CTS™Xenon™buer kits are used with the CTS™Xenon™Electroporation System for ecient
transfection of mammalian cells and are available as standalone products (see “Accessory products” on
page 69).
CTS™Xenon™buer kit components are listed in the following table, and are shipped on blue ice.
After receiving the kit, store buers at 2–8℃. Do not allow buers to undergo warming and cooling
cycles.
Item Format
CTS™Xenon™Electroporation Buer •100 mL (bottle)
•100 mL (bag)
CTS™Xenon™Genome Editing Buer •100 mL (bottle)
•100 mL (bag)
Chapter 1 Product information
Product contents 1
CTS™Xenon™Electroporation System User Guide 7

System components
CTS™Xenon™Electroporation Instrument
Front and rear view
1
2
3
4
1Door
2Touch screen
3Castor with pad and wheel (× 4)
4Grab rail (on left and right sides)
1
5
2
3
4
5
6
7
1USB wireless adaptor port
2USB 2.0 port
3Power switch
4Power cable inlet
5Fuse compartment
6Ethernet port
7Peripheral interface port (RS-232)
Chapter 1 Product information
System components
1
8CTS™Xenon™Electroporation System User Guide

Internal view
8
3
4
6
1
29
10
11
7
12
5
5
13
2
1Input bag hook
2Cartridge handle springboard
3Stopcock driver
4Input pump
5Ultrasonic sensor
6Mixing cup interface
7Mixing cup output pump
8Cooling block
9Chip reader
10 Upper electrode mount
11 Electroporation chamber holder
12 Output pump
13 Output bag tray
Chapter 1 Product information
System components 1
CTS™Xenon™Electroporation System User Guide 9

SingleShot electroporation chamber
1
4
2
3
5
1Top adaptor with authentication chip
2Upper electrode housing
3Upper electrode
4Electroporation chamber
5Lower electrode
Chapter 1 Product information
System components
1
10 CTS™Xenon™Electroporation System User Guide

MultiShot electroporation cartridge
Front
2
5
4
6
3
3
1
1Luer port for output bag
2Electroporation chamber
3Cartridge handle (on left and right sides)
4Electroporation chamber push latch
5Mixing cup (25 mL capacity)
6Luer port for input bag
Rear
1
4
2
35
5
1Mixing cup
2Sterile filters
3Electroporation chamber
4Electrode
5Cartridge handle (on left and right sides)
Chapter 1 Product information
System components 1
CTS™Xenon™Electroporation System User Guide 11

Xenon™buer kits
There are two cell resuspension buers available for use with the CTS™Xenon™Electroporation System.
•The CTS™Xenon™Electroporation Buer is designed for use with various primary cell types when
performing electroporation with standard DNA or RNA based payloads for gene expression or gene
knockdown based applications.
•The CTS™Xenon™Genome Editing Buer is designed to be used with gene editing based payloads
(Talen, Cas9) to enhance both knock-out and knock-in editing eciencies.
Upon receiving the instrument
•Check the enclosed packing list against the order.
•Visually inspect the transport package, the instrument and the accessories for any damage
incurred during transit.
•If the carton has been damaged in transit, it is particularly important that you retain it for inspection
by the carrier in case there has also been damage to the instrument.
•Any damage claims must be filed with the carrier. Neither the manufacturer nor its agents can be
held responsible for any damage incurred in transit, but the manufacturer will make every eort to
help obtain restitution from the carrier. Upon receipt of the carrier's inspection report, arrangements
will be made for repair or replacement.
•To register the instrument, activate your warranty, and be notified of important updates, go to
thermofisher.com.
Guidelines for unpacking and set up of the instrument
•To prevent condensation, the instrument should be left in its protective, antistatic plastic wrapping
until the ambient temperature has been reached.
•The instrument weighs 70 kg (154 lbs) and requires at least two people to lift. Use the proper
precautions when lifting the instrument to avoid injury.
•Retain the original packaging and packing material for future transportation. The packaging is
designed to assure safe transport and minimize transit damage. Use of alternative packaging
materials may invalidate the warranty. Also retain all instrument-related documentation provided by
the manufacturer for future use.
•Refer to the installation guide (Pub. No. 100107077) for detailed instructions on how to unpack the
instrument.
•During instrument installation and maintenance, it is necessary to access the back of the
instrument. If the back of the instrument faces a wall, ensure that there is sucient clearance
on the bench to rotate the instrument for access.
Chapter 1 Product information
Upon receiving the instrument
1
12 CTS™Xenon™Electroporation System User Guide

WARNING! Do not place the instrument adjacent to, or stack with other equipment to avoid
improper operation. If this type of placement is necessary, all the instruments and equipment should
be observed to ensure that they operate normally.
WARNING! Use of accessories, transducers, and cables other than those specified or provided by
the manufacturer of the equipment can result in increased electromagnetic emissions, or decreased
electromagnetic immunity of this equipment, and result in improper operation.
WARNING! Do not use portable RF communications equipment (including peripherals such as
antenna cables and external antennas) within 30 cm (12 inches) of any part of the instrument
(including cables specified by the manufacturer) to avoid degradation of equipment performance.
Required materials not provided
•(Optional) Electrical protective devices.
The use of one or more of the following electrical protective devices is recommended.
–Power line regulator (100–240 V)
–Surge protector/line conditioner (10-kVA)
–Uninterruptible power supply (1.5-kVA)
•USB-enabled Wi-Fi Module (for wireless connection)
Chapter 1 Product information
Upon receiving the instrument 1
CTS™Xenon™Electroporation System User Guide 13

User interface overview
Symbol Function
Main dial
Load protocol
Displays the instrument status when a protocol is run (see “Touchscreen status indicators”)
Optimization screen
Perform optimization for a new protocol (see “Optimization protocol” on page 59 for more
details)
Create protocol screen
•Create a new SingleShot protocol (see page 30 for more details)
•Create a new MultiShot protocol (see page 33 for more details)
•Edit an existing SingleShot protocol (see page 31 for more details)
•Edit an existing MultiShot protocol (see page 34 for more details)
Run previous screen
Select and run a protocol from the run history
Chapter 1 Product information
Upon receiving the instrument
1
14 CTS™Xenon™Electroporation System User Guide

Touchscreen controls
Table 1 General touchscreen controls
Button Function
Returns to the previous screen
Go to Home screen
Go to Sign in screen
Go to Settings screen
Close the current modal window.
Touchscreen status indicators
Table 2 Status indicators
Button Function
1
2
View instrument status
1. Time remaining
2. Protocol status (step, paused, etc.)
Indicates whether a USB device is inserted into the
instrument.
Indicates whether the Wi-Fi is on or o.
Indicates whether the instrument is connected to wired
network.
Indicates whether the instrument is connected to the
Thermo Fisher™Connect Platform.
Chapter 1 Product information
Upon receiving the instrument 1
CTS™Xenon™Electroporation System User Guide 15

Enter text
When you press a field that requires the input of text, the text editor, as seen in the following figure,
opens.
2 3 5 4
1 6
1Enter a letter
2Change letter case
3Enter punctuation or other symbols
4Delete
5Close and save
6Close without saving
Enter numbers
When you press a field that requires a numerical input, the numeric editor, as seen in the following
figure, opens.
2 3
4
1
1Enter a number
2Delete or enter decimal, colon or infinity
3Close and save
4Close without saving
Chapter 1 Product information
Upon receiving the instrument
1
16 CTS™Xenon™Electroporation System User Guide

First time instrument setup
1. Place the instrument in a protected location with at least 10 cm of free space around the perimeter
for ventilation.
2. Connect the power supply cable to the power inlet.
The instrument operates at voltages of 100–240 VAC and the frequency range of 50/60 Hz. Ensure
that the local supply voltage in the laboratory conforms to that specified on the type label on the
back of the instrument
Connect the instrument to the Internet
The instrument can connect to the Internet by either wired or wireless methods.
To connect by wired method through the instrument Ethernet port using a cable, see “Set up a wired
connection” on page 19.
To connect by wireless method through the instrument USB wireless adapter port with a USB-enabled
Wi-Fi card, see “Set up a wireless connection” on page 17.
Set up a wireless connection
Connect the High-Power USB Wi-Fi Module (Cat. No. A26774 to the USB wireless adapter port (see
page 8 for port location).
1. See “Set up a wired connection” on page 19 Steps 1 through 3 to find the Network
configuration screen.
2. In the Network configuration screen, select a field in the Wireless panel.
21
1Wireless panel
2Wired panel
Note: During initial setup, if you selected the Wired option in the Network Connection screen,
you will be required to enter the IP address if you selected the Static IP wired option. If you
selected the Dynamic IP wired option, the IP address is automatically populated.
Chapter 1 Product information
First time instrument setup 1
CTS™Xenon™Electroporation System User Guide 17

3. Once a wireless connection has been detected, a list of the available networks is displayed. Select
the network name of your choice or select Join others.
Note: If you choose Join others, the Configure and Join Network screen opens.
4. In the Configureand Join Network screen, select the Network Name field, then enter the name
and security type of the network.
5. Select the security type from the Security type drop-down menu.
Note: Contact your IT Systems Administrator for information on security type.
Select from the following options:
•Open
•WEP
•WPA Personal
•WPA2 Personal
•WPA Enterprise
•WPA2 Enterprise
Note: The above options are available only if Join Other Network was selected in Step 3. You
cannot change the security type if you selected an existing network.
6. Select Join to continue or Cancel to exit from the Find and Join a Network screen.
7. Depending on the security type you have selected, enter the appropriate passwords and select
Join.
8. If all the entered information is correct, the Network Connection Complete screen will appear.
Select OK to continue.
Note: If incorrect information was entered the Network Connection Failed screen will open.
Select OK to continue to the Security type screen.
Chapter 1 Product information
First time instrument setup
1
18 CTS™Xenon™Electroporation System User Guide

Set up a wired connection
Connect one end of a Ethernet cable to the instrument Ethernet port, and the other end to an Ethernet
port wall plug (see page 8 for port location).
1. On the Home screen, select (Settings).
2. In the Settings screen, select Instrument Settings.
Chapter 1 Product information
First time instrument setup 1
CTS™Xenon™Electroporation System User Guide 19

3. In the Instrument Settings screen, select Network configuration.
4. In the Network Connection screen, select a field in the Wired panel.
21
1Wireless panel
2Wired panel
5. Select a method to enter an IP address.
a. Select DHCP to obtain an IP address automatically. A check mark appears when DHCP is
selected.
Chapter 1 Product information
First time instrument setup
1
20 CTS™Xenon™Electroporation System User Guide