Thermo Scientific CTS Xenon User manual

For Research Use or Manufacturing of Cell, Gene, or Tissue- Based
Products. CAUTION: Not intended for direct administration into
humans or animals.
CTS™Xenon™Electroporation System
USER GUIDE
For electroporation of primary and stem cells for cell therapy
development and manufacturing
Catalog Number A52727
Publication Number MAN0025488
Revision A.0

Life Technologies Holdings Pte Ltd | Block 33 | Marsiling Industrial Estate Road 3 | #07-06, Singapore 739256
For descriptions of symbols on product labels or product documents, go to thermofisher.com/symbols-definition.
The information in this guide is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC INC. AND/OR ITS AFFILIATE(S) WILL NOT BE
LIABLE FOR SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE, OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR
ARISING FROM THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history: Pub. No. MAN0025488
Revision Date Description
A.0 11 November 2021 New user guide for CTS™Xenon™Electroporation Instrument.
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
TRADEMARKS: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
©2021 Thermo Fisher Scientific Inc. All rights reserved.

Contents
■CHAPTER 1 Product information .................................................. 6
Product description ............................................................. 6
Product contents ................................................................ 7
CTS™Xenon™system contents ............................................... 7
CTS™Xenon™buer kit contents ............................................. 7
System components ............................................................. 8
CTS™Xenon™Electroporation Instrument ...................................... 8
SingleShot electroporation chamber ......................................... 10
MultiShot electroporation cartridge .......................................... 11
Xenon™buer kits ......................................................... 12
Upon receiving the instrument ................................................... 12
Guidelines for unpacking and set up of the instrument ......................... 12
Required materials not provided ............................................. 13
User interface overview ..................................................... 14
Touchscreen controls ...................................................... 15
First time instrument setup ...................................................... 17
Connect the instrument to the Internet ....................................... 17
Create a user profile on the instrument ....................................... 21
Manage user profiles ....................................................... 22
About the Thermo Fisher™Connect Platform ...................................... 23
Create a Connect account .................................................. 23
Link the instrument to Connect (Administrator only) ............................ 24
Connect the instrument to the Internet ....................................... 26
Create a PIN number ....................................................... 26
Generate a link code from the instrument ..................................... 26
Set up a new Administrator ................................................. 26
Add an instrument to your Connect account .................................. 27
CTS™Xenon™Electroporation System User Guide 3

■CHAPTER 2 Methods ............................................................. 29
Electroporation protocol options ................................................. 29
Electroporation parameters ................................................. 29
Create/Edit SingleShot protocols ................................................. 30
Create SingleShot Protocol ................................................. 30
Edit SingleShot Protocol .................................................... 31
Create/Edit MultiShot protocols .................................................. 33
Create MultiShot Protocol .................................................. 33
Edit MultiShot Protocol ..................................................... 34
Convert a SingleShot protocol to a MultiShot protocol ......................... 34
Save a Protocol ................................................................ 35
Import protocols ............................................................... 35
Manage protocols .............................................................. 35
General guidelines ............................................................. 36
Guidelines for electroporation payloads ...................................... 36
Controls .................................................................. 37
Required materials ............................................................. 37
Prepare cells .................................................................. 38
Activate T cells ............................................................ 38
Prepare cells for electroporation ............................................. 38
Perform electroporation ......................................................... 40
Run SingleShot protocol .................................................... 40
Run MultiShot protocol ..................................................... 42
Post-electroporation procedure .............................................. 46
Maintenance .................................................................. 47
Cleaning and maintenance .................................................. 47
Replace the fuses .......................................................... 48
Upgrade the system firmware ............................................... 48
Self Verification test ........................................................ 51
Restore factory settings (Administrator profile only)............................ 51
Repackaging the instrument ................................................ 52
■APPENDIX A Troubleshooting .................................................... 53
Troubleshooting ................................................................ 53
Remove jammed electroporation chamber ........................................ 55
Remove the plastic cover ................................................... 55
Error codes ................................................................... 57
■APPENDIX B Optimization ........................................................ 59
Optimization protocol ........................................................... 59
Contents
4CTS™Xenon™Electroporation System User Guide

■APPENDIX C Recommended instrument settings .............................. 60
About Instrument .............................................................. 60
Recommended instrument settings .............................................. 60
Instrument settings (Administrator only) ...................................... 61
Maintenance & services ......................................................... 65
Run History ................................................................... 66
■APPENDIX D Specifications ...................................................... 68
Product specifications .......................................................... 68
■APPENDIX E Related products ................................................... 69
Accessory products ............................................................ 69
Additional products ........................................................ 69
■APPENDIX F Safety ............................................................... 71
Safety information .............................................................. 71
Safety compliance ............................................................. 72
Informational symbols .......................................................... 72
Symboles d’information ......................................................... 73
Environmental requirements ..................................................... 74
Electromagnetic compatibility (EMC) standards .................................... 75
Class A notice ............................................................. 75
EMC compliance .......................................................... 75
Electrical safety ................................................................ 76
Chemical safety ................................................................ 77
Biological hazard safety ......................................................... 78
■APPENDIX G Documentation and support ...................................... 79
Customer and technical support ................................................. 79
Limited product warranty ........................................................ 79
Contents
CTS™Xenon™Electroporation System User Guide 5

Product information
Product description
The CTS™Xenon™Electroporation System is a novel, benchtop device consisting of the CTS™Xenon™
Electroporation Instrument, single-use consumables for performing transfection of cells, and specialized
buers.
The CTS™Xenon™Electroporation Instrument eciently delivers gene editing payloads (DNA, RNA
and proteins) into all mammalian cell types including primary and stem cells with a high cell survival
rate. Protocols are optimized for ease of use and simplicity, and conditions can be optimized for your
payload and cell type. See “System components” on page 8 for details on various parts of the
system.
Two types of consumable are used for performing transfection.
• The SingleShot format uses an electroporation chamber to process 2 × 107to 1 × 108cells in a
1 mL sample volume. See “SingleShot electroporation chamber” on page 10.
• The MultiShot format uses a cartridge and sample bags to process 1 × 108to 2.5 × 109cells in a
5–25 mL sample volume. See “MultiShot electroporation cartridge” on page 11
The system includes proprietary buers that are compatible with various cell types including primary
lymphocytes and stem cells. See “Xenon™buer kits” on page 12
•The CTS™Xenon™Electroporation Buer is compatible with a wide variety of mammalian cell
types, eliminating the need to determine an optimal buer for each cell type.
•The CTS™Xenon™Genome Editing Buer is designed to improve performance with gene editing
specific payloads (Talen, Cas9) in mammalian primary and stem cells.
1
6CTS™Xenon™Electroporation System User Guide

Product contents
CTS™Xenon™system contents
The contents of the CTS™Xenon™Electroporation System are listed in the following table. The system
is shipped at room temperature.
See page 8 for specifications and description of the CTS™Xenon™Electroporation Instrument, and
page 29 to set up the instrument.
Product Quantity
CTS™Xenon™Electroporation Instrument 1
Region specific power cord
(for US/Canada/Taiwan/Japan, Europe, or UK)
1
Cat 5 Ethernet cable 1
USB memory device (containing user guide) 1
Quick reference 1
Install guide 1
Bubble level 1
CTS™Xenon™buer kit contents
The CTS™Xenon™buer kits are used with the CTS™Xenon™Electroporation System for ecient
transfection of mammalian cells and are available as standalone products (see “Accessory products” on
page 69).
CTS™Xenon™buer kit components are listed in the following table, and are shipped on blue ice.
After receiving the kit, store buers at 2–8℃. Do not allow buers to undergo warming and cooling
cycles.
Item Format
CTS™Xenon™Electroporation Buer •100 mL (bottle)
•100 mL (bag)
CTS™Xenon™Genome Editing Buer •100 mL (bottle)
•100 mL (bag)
Chapter 1 Product information
Product contents 1
CTS™Xenon™Electroporation System User Guide 7

System components
CTS™Xenon™Electroporation Instrument
Front and rear view
1
2
3
4
1Door
2Touch screen
3Castor with pad and wheel (× 4)
4Grab rail (on left and right sides)
1
5
2
3
4
5
6
7
1USB wireless adaptor port
2USB 2.0 port
3Power switch
4Power cable inlet
5Fuse compartment
6Ethernet port
7Peripheral interface port (RS-232)
Chapter 1 Product information
System components
1
8CTS™Xenon™Electroporation System User Guide

Internal view
8
3
4
6
1
29
10
11
7
12
5
5
13
2
1Input bag hook
2Cartridge handle springboard
3Stopcock driver
4Input pump
5Ultrasonic sensor
6Mixing cup interface
7Mixing cup output pump
8Cooling block
9Chip reader
10 Upper electrode mount
11 Electroporation chamber holder
12 Output pump
13 Output bag tray
Chapter 1 Product information
System components 1
CTS™Xenon™Electroporation System User Guide 9

SingleShot electroporation chamber
1
4
2
3
5
1Top adaptor with authentication chip
2Upper electrode housing
3Upper electrode
4Electroporation chamber
5Lower electrode
Chapter 1 Product information
System components
1
10 CTS™Xenon™Electroporation System User Guide