NEW BRUNSWICK SCIENTIFIC CO28IR User manual

CO28IR
Water-Jacketed
CO2 Incubator
with HEPA filtration
M1271–0051
Revision A
January 20, 2004
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 •44 TALMADGE ROAD •EDISON, NJ 08818-4005
Telephone: [phone] •1-800-631-5417
Fax: [phone] •Telex: 4753012 NBSCO
Internet: http://www.nbsc.com •E-mail: [email protected]

ii
CO28IR CO2Incubator with HEPA M1271-0051 User’s Manual

iii
New Brunswick Scientific User’s Manual
INTERNATIONAL OFFICES:
BELGIUM
New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
GERMANY
New Brunswick Scientific GmbH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
CHINA
New Brunswick Scientific Co., Inc.
Room 1501, Xiangjiang Building, No. 18
Lane 1265, Zhongshan Road (W)
Shanghai 200051, P.R. China
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
THE NETHERLANDS
New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
P.O Box 6826, 6503 GH Nijmegen
Nederland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
FRANCE
New Brunswick Scientific SARL
3, rue des Deux-Boules
75001 Paris
France
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
UNITED KINGDOM
New Brunswick Scientific (UK) Ltd.
17 Alban Park
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: [phone] or 0800 581331
Fax: [phone]
E-mail: [email protected]
Web: www.nbsuk.co.uk

iv
CO28IR CO2Incubator with HEPA M1271-0051 User’s Manual
THIS PAGE IS INTENTIONALLY BLANK

v
New Brunswick Scientific User’s Manual
CAUTION!
This equipment must be operated as described in this
manual. If operational guidelines are not followed,
equipment damage and personal injury can occur.
Please read the entire User’s Guide before attempting to
use this unit.
Do not use this equipment in a hazardous atmosphere or
with hazardous materials for which the equipment was not
designed.
New Brunswick Scientific Co., Inc. (NBS) is not responsible
for any damage to this equipment that may result from the
use of an accessory not manufactured by NBS.

vi
CO28IR CO2Incubator with HEPA M1271-0051 User’s Manual
THIS PAGE IS INTENTIONALLY BLANK

vii
New Brunswick Scientific User’s Manual
Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005 44 Talmadge Road
Edison, New Jersey 08818-4005
Copyright 2004 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission
from
New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in
this document reflect our commitment to continuing product
development and improvement.
Manual Conventions
NOTE:
CAUTION!
WARNING!
Bold Text in bold face type emphasizes key words or
phrases.
Caution messages appear before procedures
which, if caution is not observed, could result
in damage to the equipment.
Notes contain essential information that
deserves special attention.
Warning messages alert you to specific
procedures or practices which, if not followed
correctly, could result in serious personal
injury.

viii
CO28IR CO2Incubator with HEPA M1271-0051 User’s Manual
THIS PAGE IS INTENTIONALLY BLANK

ix
New Brunswick Scientific User’s Manual
Every Instrument manufactured by the
New Brunswick Scientific Co., Inc. is warranted to be free from
defects in material and workmanship. This apparatus, with the
exception of glassware, lamps and electrodes (where supplied), is
warranted for 1 year against faulty components and assembly and our
obligation under this warranty is limited to repairing or replacing the
instrument or part thereof, which shall within 1 year after date of
shipment, prove to be defective after our examination. This
warranty does not extend to any NBS products which have been
subjected to misuse, neglect, accident or improper installation
or application; nor shall it extend to products which have
been repaired or altered outside the NBS factory
without prior authorization from the
New Brunswick Scientific Co., Inc.
W A R R A N T Y

x
CO28IR CO2Incubator with HEPA M1271-0051 User’s Manual
THIS PAGE IS INTENTIONALLY BLANK

xi
New Brunswick Scientific User’s Manual
TABLE OF CONTENTS
1RECEIVING AND INSPECTION..................................................................................1
1.1 INSPECTION.................................................................................................................. 1
1.2 RETURN SHIPMENT ......................................................................................................1
2GRAPHIC SYMBOLS ..................................................................................................... 3
3INSTALLATION..............................................................................................................5
3.1 POWER SUPPLY............................................................................................................ 5
3.2 LOCATION, HANDLING & LEVELING ...........................................................................5
3.3 STACKING ....................................................................................................................6
3.4 CLEANING.................................................................................................................... 7
3.5 INSTALLING SHELVES & HUMIDITY PAN .....................................................................8
3.6 INSTALLING FAN COVER & HEPA FILTER ..................................................................9
4CONTROLS....................................................................................................................11
4.1 FRONT CONTROL PANEL............................................................................................ 11
4.2 DOOR TEMPERATURE CONTROL ................................................................................12
4.3 POWER CORD INLET ..................................................................................................13
4.4 HEPA CO2FILTER ....................................................................................................13
5OPERATION ..................................................................................................................15
5.1 POWERING UP............................................................................................................15
5.2 FILLING THE WATER JACKET .....................................................................................15
5.3 SETTING THE CONTROLS............................................................................................15
5.4 CALIBRATING THE CONTROLS ...................................................................................16
5.5 HUMIDIFICATION .......................................................................................................16
5.6 TEMPERATURE MONITORING .....................................................................................17
5.7 INITIAL CONTROL SETTING........................................................................................17
5.8 TEMPERATURE CALIBRATION ....................................................................................17
5.9 CO2SUPPLY SYSTEM & CONTROL SYSTEM...............................................................18
5.10 SETTING THE CO2CONTROL...................................................................................... 20
5.11 ADJUSTING THE CO2DISPLAY ...................................................................................20
5.12 ALARMS..................................................................................................................... 20
5.13 DRAINING THE WATER JACKET .................................................................................21
6FYRITE CO2CHECKING............................................................................................23
7MAINTENANCE............................................................................................................25
7.1 CLEANING.................................................................................................................. 25
7.2 MAINTENANCE ..........................................................................................................25
7.3 REPLACING THE INTERNAL CO2FILTER.....................................................................26
7.4 REPLACING THE HEPA FILTER.................................................................................. 26
7.5 STORAGE ...................................................................................................................27
8TROUBLESHOOTING .................................................................................................29

xii
CO28IR CO2Incubator with HEPA M1271-0051 User’s Manual
9PARTS LIST ...................................................................................................................37
10 UNIT SPECIFICATIONS .........................................................................................39
11 WIRING DIAGRAMS ...............................................................................................41
11.1 LIST OF DRAWINGS ....................................................................................................47
11.2 LIST OF TABLES .........................................................................................................47
12 INDEX .........................................................................................................................49
NOTE:
These CO2water-jacketed incubators are for professional, industrial or
educational use where the preparation or testing of materials is done at
approximately atmospheric pressure and where no flammable, volatile or
combustible materials are being heated. These units are not intended for
hazardous or domestic use.

1
New Brunswick Scientific User’s Manual
1
1 R RE
EC CE EI IV VI IN NG G A AN ND D I
IN
NS SP PE EC CT TI IO ON N
Your satisfaction and safety require a complete understanding of this unit. Read the instructions
provided in this manual thoroughly, and be sure all operators are given adequate training before
attempting to put the unit in service.
NOTE:
This equipment must be used only for its intended application; any alterations
or modifications will void your warranty.
1.1 Inspection
The carrier, when accepting shipment, also accepts responsibility for safe delivery and
is liable for loss or damage. On delivery, inspect for visible exterior damage, note and
describe on the freight bill any damage found, and enter your claim on the form
supplied by the carrier.
Inspect for concealed loss or damage on the unit itself, both interior and exterior. If
necessary, the carrier will arrange for official inspection to substantiate your claim.
Verify that all of the equipment indicated on the packing list is included with the unit. If
anything is missing, contact NBS customer service.
We recommend that you save the packing materials for possible future use (safe transport of
the incubator).
1.2 Return Shipment
If for any reason you must return the unit, first contact your customer service
representative for a return authorization number. Supply nameplate data, including
model number and serial number. The return authorization number must be plainly
marked on any item(s) you return. Use the original shipping crate and packing
materials for safe transport.

2
CO28IR CO2Incubators with HEPA M1271-0051 User’s Manual
THIS PAGE IS INTENTIONALLY BLANK

3
New Brunswick Scientific User’s Manual
2
2 G GR
RA AP PH HI IC C S
SY
YM MB BO OL LS S
Your incubator has been provided with a display of graphic symbols that are designed to help
in identifying the use and function of the available user-adjustable components. The table
below will identify the meaning of those symbols:
Table 1: Control Panel Symbols
Indicates that you should consult your manual for further description or
discussion of a control or user item.
Indicates “Temperature”.
Indicates “Over Temperature Safety”.
Indicates “Manual Adjustment”.
CO2%
Indicates “Percent Carbon Dioxide”.
Indicates “Gas” (CO2for this unit).
Indicates “AC Power”.
Indicates “Water Jacket Low”.
Indicates “Protective Earth Ground”.
Indicates “Relative Humidity”.
%RH
Indicates “Percent Relative Humidity”.
Indicates “Potential Shock Hazard” behind partition.

4
CO28IR CO2Incubators with HEPA M1271-0051 User’s Manual
THIS PAGE IS INTENTIONALLY BLANK

5
New Brunswick Scientific User’s Manual
3
3 I IN
NS ST TA AL LL LA AT TI IO ON N
The end user can easily install the unit. Local city, county or other ordinances may govern the
use of this equipment. If you have any questions about local requirements, please contact the
appropriate local agency.
Under normal circumstances, this unit is intended for indoor use, at room temperature
between 5°and 40°C, with no more than 80% Relative Humidity at 25°C, and with a supply
voltage that does not vary by more than 10%. Be sure to contact NBS Customer Service if
you anticipate operating conditions outside these limits.
3.1 Power Supply
Electrical supply to the incubator must conform to all national and local electrical
codes. Before you plug it into the power supply, consult the incubator’s serial data
plate for its voltage, cycle and ampere requirements. VOLTAGE SHOULD NOT
VARY MORE THAN 10% FROM THE RATING on the serial plate.
This unit is intended for 50/60 Hz application. A separate circuit is recommended to
prevent possible loss of product due to overloading or failure of any other equipment
on the same circuit.
3.2 Location, Handling & Leveling
In selecting a location, consider all conditions that might affect performance, such as heat
from radiators, ovens, autoclaves, etc. Avoid direct sunlight, fast-moving air currents,
heating/cooling ducts and high-traffic areas. Allow a minimum of 5 cm between the unit
and any walls or partitions that might obstruct free air flow.
These units are heavy, so care should be taken to use appropriate lifting devices that are
sufficiently rated for these loads. Lift the units only from the bottom surface; doors,
handles and knobs are not adequate for lifting or for stabilization. Completely restrain
each unit from tipping during lifting or transport. Remove all movable parts, such as
shelves and trays. Lock the doors in the closed position during transfer to prevent
shifting and damage.
CAUTION!
Make sure that the incubator is located in its intended position
and is level before filling the water jacket.

6
CO28IR CO2Incubators with HEPA M1271-0051 User’s Manual
The unit must sit solidly and be level. The leveling feet supplied with the incubator
must be installed in the four holes in the bottom corners of the unit, unless you are
going to stack it (see Section 3.3).
With the feet installed and the unit standing upright, each foot can be raised by turning
it in a counterclockwise direction. Adjust the foot at each corner until the unit stands
solidly level, without rocking. If the unit must be moved, turn the leveling feet
clockwise all the way to prevent damage while moving.
3.3 Stacking
Two CO28IR incubators may be stacked using the optional NBS stacking kit (PN
M1271-9990). See the procedure outlined in Figure 1.
These units are heavy, so care should be taken to use appropriate lifting devices that are
sufficiently rated for these loads. Lift the units only from the bottom surface; doors,
handles and knobs are not adequate for lifting or for stabilization. Completely restrain
each unit from tipping during lifting or transport. Remove all movable parts, such as
shelves and trays. Lock the doors in the closed position during transfer to prevent
shifting and damage.
WARNING!
Unplug units from power before beginning the stacking
procedure.

7
New Brunswick Scientific User’s Manual
Figure 1: Stacking Units
3.4 Cleaning
The unit chamber should be cleaned and disinfected prior to use. Remove all of the
interior parts and clean thoroughly, including all corners, using a suitable disinfectant
that is appropriate to your application.
Do not use cleaning solution on the HEPA filter. Be sure to replace the HEPA filter
periodically (see Section 7.4).
WARNING!
Never clean the unit while it is connected to the electrical supply,
especially when using alcohol or flammable cleaners.
Always disconnect the unit from the electrical service when cleaning
and assure all volatile or flammable cleaners are evaporated and dry
before reattaching the unit to the power supply.
1. Remove adjustable feet
from the bottom of Unit 1,
and remove the blanking
plugs from the top surface
of Unit 2.
2. Using an appropriate lifting
device, place Unit 1 on top
of Unit 2.
3. Unscrew the two retaining
screws to remove the back
access cover of Unit 2.
This allows access into the
upper area of Unit 2.
4.
A
lign the holes in the top of
Unit 2 with the threaded
foot holes in the bottom of
Unit 1.
5. Install the four 5/16” x 18
bolts, washers and lock
washers from inside Unit 2
up into foot nuts of Unit 1.
Tighten snugly.
6. Replace access cover on
Unit 2 using the two
retaining screws previously
removed.
OPTIONAL STACKING KIT
PN M1271-9990

8
CO28IR CO2Incubators with HEPA M1271-0051 User’s Manual
Regular periodic cleaning is required. Special care should be taken when cleaning
around sensing heads to prevent damage. DO NOT USE chlorine-based bleaches or
abrasive cleaners, as this will damage the stainless steel interior; or spray cleaners that
might leak through openings, getting on electrical parts; or cleaners that contain
solvents that can harm the coatings.
3.5 Installing Shelves & Humidity Pan
Shelving, shelf supports and a humidity pan are supplied with each unit. See Figure 2
and the instructions that follow for proper installation of these items, and Figure 2a for
a photograph of the finished installation.
Figure 2: Shelf Installation
Place the humidity pan inside the incubator, in the center of the floor.
See Figure 2a on the following page for a photograph of the shelves, fan cover, HEPA
filter and humidity pan in place.
Install the four shelf
standards: position the
keyholes over the pins
in the wall, then slide
the standard down,
fixing the pins in the
grooves.
Install the shelf sliders:
Align the ends of each slider
with a slot in the shelf standards.
Angle the slider toward the
slot in the standard, and lift
upward until the slider contacts
the top of the slot.
& Rotate the bottom of the
slider toward the shelf standard
to complete in installation.
Repeat these steps for each shelf
slider.



