NEW BRUNSWICK SCIENTIFIC CO-150 User manual

Model CO-150
CO2Incubator
MANUAL NO: M1310-0050
Revision A
April 17, 2004
NEW BRUNSWICK SCIENTIFIC CO., INC.
BOX 4005 •44 TALMADGE ROAD •EDISON, NJ 08818-4005
Telephone: [phone] •1-800-631-5417
Fax: [phone] •Telex: 4753012 NBSCO
Internet: http://www.nbsc.com •E-mail: [email protected]

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New Brunswick Scientific User’s Guide
INTERNATIONAL OFFICES:
BELGIUM
New Brunswick Scientific NV-SA
Stationsstraat 180/4
3110 Rotselaar
België/Belgique
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
GERMANY
New Brunswick Scientific GmbH
In Der Au 14
D-72622 Nürtingen
Deutschland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
CHINA
New Brunswick Scientific Co., Inc.
Room 1501, Xiangjiang Building, No. 18
Lane 1265, Zhongshan Road (W)
Shanghai 200051, P.R. China
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
THE NETHERLANDS
New Brunswick Scientific BV
Kerkenbos 1101, 6546 BC Nijmegen
P.O Box 6826, 6503 GH Nijmegen
Nederland
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
FRANCE
New Brunswick Scientific SARL
3, rue des Deux-Boules
75001 Paris
France
Tel: [phone]
Fax: [phone]
E-mail: [email protected]
UNITED KINGDOM
New Brunswick Scientific (UK) Ltd.
17 Alban Park
St. Albans, Herts. AL4 0JJ
United Kingdom
Tel: [phone] or 0800 581331
Fax: [phone]
E-mail: [email protected]
Web: www.nbsuk.co.uk

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New Brunswick Scientific User’s Guide
CAUTION!
This equipment must be operated as described in this
manual. If operational guidelines are not followed,
equipment damage and personal injury can occur.
Please read the entire User’s Guide before attempting to
use this unit.
Do not use this equipment in a hazardous atmosphere or
with hazardous materials for which the equipment was not
designed.
New Brunswick Scientific Co., Inc. (NBS) is not responsible
for any damage to this equipment that may result from the
use of an accessory not manufactured by NBS.

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New Brunswick Scientific User’s Guide
Copyright Notice
New Brunswick Scientific Company, Inc.
Box 4005, 44 Talmadge Road
Edison, New Jersey 08818-4005
Copyright 2004 New Brunswick Scientific Co., Inc.
All Rights Reserved.
Reproduction, adaptation, or translation without prior written permission
from New Brunswick Scientific is prohibited.
Disclaimer Notice
New Brunswick Scientific Co., Inc. reserves the right to change
information in this document without notice. Updates to information in
this document reflect our commitment to continuing product
development and improvement.
Manual Conventions
NOTE:
CAUTION!
WARNING!
Bold
Caution messages appear before procedures
which, if caution is not observed, could result
in damage to the equipment.
Notes contain essential information that
deserves special attention.
Warning messages alert you to specific
procedures or practices which, if not followed
correctly, could result in serious personal
injury.
Text in bold face type emphasizes key words or
phrases.

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New Brunswick Scientific User’s Guide
Every Instrument manufactured by the
New Brunswick Scientific Co., Inc. is warranted to be free
from defects in material and workmanship. This apparatus, with
the exception of glassware, lamps and electrodes (where supplied),
is warranted for 1 year against faulty components and assembly and
our obligation under this warranty is limited to repairing or replacing
the instrument or part thereof, which shall within 1 year following
date of shipment prove to be defective after our examination.
This warranty does not extend to any NBS products which have
been subjected to misuse, neglect, accident or improper
installation or application; nor shall it extend to products
which have been repaired or altered outside the NBS
factory without prior authorization from the
New Brunswick Scientific Co., Inc.
W A R R A N T Y

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New Brunswick Scientific User’s Guide
TABLE OF CONTENTS
1INTRODUCTION ........................................................................................................ 13
2UNPACKING & INSTALLATION ........................................................................... 15
2.1 INSPECTION OF BOXES............................................................................................. 15
2.2 UNPACKING ............................................................................................................. 15
2.3 UTILITIES................................................................................................................. 16
2.4 LOCATION ............................................................................................................... 16
2.5 INSTALLING THE FEET ............................................................................................. 17
2.6 SETTING UP............................................................................................................. 17
3OPERATION................................................................................................................ 19
3.1 CONTROL PANEL ..................................................................................................... 19
3.2 PREPARING FOR OPERATION.................................................................................... 20
3.3 USING THE HUMIDITY TRAY.................................................................................... 21
3.4 SETTING TEMPERATURE & CO2.............................................................................. 21
3.5 REFERENCING CO2 WITH AUTOZERO ...................................................................... 22
3.6 PROGRAMMING THE ALARM SYSTEM ...................................................................... 23
3.6.1 Setting High & Low Temperature Alarms...................................................... 23
3.6.2 Setting the CO2 High & Low Alarms .............................................................. 23
3.6.3 Door Open Alarm ........................................................................................... 24
3.6.4 Alarm Duration .............................................................................................. 24
3.6.5 Alarm Arming Delay....................................................................................... 24
3.7 CHAMBER ALARM SYSTEM ..................................................................................... 25
3.7.1 Temperature Sensor System Alarms............................................................... 27
3.7.2 Over-Temperature Cut-out & Alarm.............................................................. 27
3.7.3 CO2Control System Alarm............................................................................. 28
4ROUTINE MAINTENANCE...................................................................................... 29
4.1 GENERAL NOTES ..................................................................................................... 29
4.2 DAILY CHECKS........................................................................................................ 29
4.3 MONTHLY CHECKS.................................................................................................. 29
5CLEANING & DISINFECTING ................................................................................ 31
5.1 CLEANING ............................................................................................................... 31
5.2 DISINFECTING.......................................................................................................... 31
6SPECIFICATIONS ...................................................................................................... 33
7OPTIONS & ACCESSORIES..................................................................................... 35
7.1 OPTIONS .................................................................................................................. 35
7.2 AVAILABLE ACCESSORIES ....................................................................................... 35
8DRAWINGS & TABLES............................................................................................. 37
8.1 LIST OF DRAWINGS.................................................................................................. 37
8.2 LIST OF TABLES....................................................................................................... 37

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9INDEX ...........................................................................................................................39

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New Brunswick Scientific User’s Guide
1
1 I IN
NT TR RO OD DU UC CT TI IO ON N
The model CO-150 CO2incubator is microprocessor-controlled and designed to ensure
accurate and reliable operation. The unit incorporates a simple, door-mounted touch-
sensitive keypad with two individual three-digit LED displays that allow for easy
programming and monitoring of the chamber conditions.
A direct heat system, utilizing a thermal heating element, completely surrounds the
incubator, providing an even temperature within the chamber. The independently heated
outer door is designed to ensure an even distribution of heat, thereby eliminating
condensation on the inner door. This system ensures a rapid, controlled return to optimum
chamber conditions after a door opening while also preventing any overshoot.
A solid-state infrared sensor is used to control the level of CO2, providing excellent
reliability and remaining unaffected by humidity. The CO2system has a semi-automatic
zero function (“AutoZero”) that provides a simple process to maintain an accurate level of
CO2within the chamber.
The 150-liter chamber and all internal components are manufactured from polished stainless
steel. The non-tip shelves, shelf racks and humidity tray are easily removed without tools for
thorough cleaning and are capable of being sterilized. Air circulation is achieved without the
use of a fan, eliminating ductwork (a potential source of contamination), simplifying
cleaning, eliminating vibration and reducing small sample evaporation within the chamber.
The outer shell of the incubator is manufactured from zinc-plated stove enamelled steel to
give a durable corrosion-resistant finish.
The incubator incorporates a two-level alarm system. The chamber-monitoring alarms are
programmable and will alert you if temperature or CO2have not recovered within a preset
time after the door has been opened. If it is not required, this system can be disarmed. The
system alarms occur only if a problem has developed with system components that require
user intervention to rectify. The incubator also incorporates an over-temperature safety
system that operates independently from the main control system.
The incubator’s direct heat system was designed with optimal use of laboratory space in
mind: it allows the most efficient internal volume for the footprint of the instrument. In
addition, the incubator’s top panel is specifically designed to support the weight of a second
identical incubator stacked directly on top of the first.

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New Brunswick Scientific User’s Guide
2
2 U UN
NP PA AC CK KI IN NG G &
& I IN
NS ST TA AL LL LA AT TI IO ON N
2.1 Inspection of Boxes
After you have received your order from New Brunswick Scientific, inspect the
boxes carefully for any damage that may have occurred during shipping. Report any
damage to the carrier and to your local NBS Sales Order Department or distributor
immediately.
2.2 Unpacking
Disassemble the wooden shipping crate and remove the protective packing. Save the
packing materials for possible future use, and be sure to save this User’s Guide for
instruction and reference.
To simplify lifting the incubator, leave it on the pallet. You must have at least one
person at either side to safely lift the unit. Supported by the base only, remove the
incubator by lifting it from the delivery pallet.
Locate and remove the parts stored in the Humidity Tray.
If any part of your order was damaged during shipping, is missing, or fails to operate,
please fill out Customer Satisfaction Form 6300 (packed in the envelope with your
warranty card) and return it by fax or mail. You can also call New Brunswick
Scientific’s or your distributor’s service department.
Using your NBS packing list, verify that you have received the correct materials, and
that nothing is missing.
Table 1 on the following page outlines the accessory items that are supplied with
your new incubator:
WARNING!
At least two people are required for to safely lift your CO-150.

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M1310-0050 Model CO-150 CO2Incubator User’s Guide
Table 1: Accessories Provided
Quantity Item Notes
3 Non-tip Shelves Packed Separately
2 Wire Shelf Racks (one for each side) Packed Separately
14 Silicone Rubber Suction Feet Packed in Humidity Tray
1 Humidity Tray Packed Separately
1 White porous CO2Sensor Cover Installed
1 Black Sensor Cover Installed
1 Power Cord Packed in Humidity Tray
3 meters,
9.8 feet
PVC Tubing, ~1/4-inch or 6mm bore,
with an inline CO2HEPA-filter
connected, ready for use
Packed in Humidity Tray
2 Hose Clips Packed in Humidity Tray
4 Adjustable Feet, with locking nuts Packed in Humidity Tray
4 Anti-slip Pads for adjustable feet Packed in Humidity Tray
1 User Manual Supplied
2.3 Utilities
In order to use the incubator, you will need:
Table 2: Utilities
Utility Requirement
110/120V, 50/60 Hz grounded electrical supply with min. capacity of 6 amps
OR
Electricity
220/240V, 50 Hz grounded electrical supply with minimum capacity of 3 amps
CO2Gas Cylinder with 100% CO2vapor withdrawal, together with a two-stage regulator
for pressure control to 5 psi or 0.35 BAR
2.4 Location
The incubator is designed to operate at a chamber temperature of 1.0°C above
ambient, and at an absolute minimum ambient temperature of 15°C if the unit is
being used at 37°C. Care should be taken to avoid placing the incubator in a position
that may affect its performance, such as those listed below.
DO NOT place the incubator:
• Directly under, beside or within the air-flow of heating or air-
conditioning ducts, or other drafts;
• Directly beside heat generating equipment such as a heater, an autoclave
or oven;
• Near the exhaust of heat- or cold-generating equipment (like a ULT
freezer);

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New Brunswick Scientific User’s Guide
• Near a window exposed to direct, unshaded sunlight.
Remove the unit from the pallet and place the incubator in the working position, on a
level surface capable of bearing its weight (approximately 163 lbs./74 kg; actual use
weight will be heavier and will depend on the options installed and the material
stored in the incubator).
The incubator is designed so that one incubator can be safely stacked on top of
another identical unit. It is not possible to put any other type of incubator or heavy
apparatus on top, as the top cover is designed to support only the feet of another
model CO-150.
NOTE:
There is no need to remove the top panel for normal maintenance or
servicing, so the upper unit does not have to be moved when you are
servicing the lower unit.
2.5 Installing the Feet
To ensure adequate airflow for correct operation of the relative humidity control
system, the incubator feet must be installed. More than one person is required to
perform this operation.
To insert the adjustable feet:
1. Make sure the locking nuts are installed onto each of the four feet provided.
2. Beginning with the front pair: tilt the incubator toward the back, and screw the
feet in, to the required depth.
3. Tilt the incubator forward to install the rear pair of feet.
4. Put an anti-slip pad (provided) on each foot.
2.6 Setting Up
Install the power cord:
1. Insert the power cord into its receptacle on the back of the unit (see Figure 3 below).
2. Press the cord firmly into its socket.
Install the wire shelf racks and shelves and level the incubator:
1. Each wire shelf rack has silicone suction cups that hold the rack in place. Place
one silicone suction cup onto the end of each of the wire rack supports (each
marked with an asterisk in Figure 1a below). Be sure to install the suction cup
marked Ras indicated in this illustration.

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M1310-0050 Model CO-150 CO2Incubator User’s Guide
R
*
*
*
*
*
*
*
2. Place the racks inside the chamber, one on each side (they will only fit correctly
one way). Two suction cups sit on the bottom of the incubator chamber, and the
one marked R(“Rear”) is placed against the back wall of the chamber.
Figure 1: Installing Shelf Racks & Shelves
a b
3. Install the 3 shelves (Figure 1b above). Make sure that each shelf’s anti-tip
groove is properly inserted onto each of the wire shelf rack guides.
4. Level the incubator by adjusting the feet. Place a small level on the second shelf
of the incubator. Adjust the leveling feet until the incubator is level and stable.
Lock the leveling feet in place by tightening the locking nuts on each foot.
Install the humidity tray:
1. Install the humidity tray onto the lowest supports of the wire shelf racks (see
Figure 1b above).
2. Ensure that the humidity tray is pushed fully back against the rear wall. The tray
is designed so that it is always in the optimum position for condensate collection.
Connect the CO2gas supply:
1. Connect the unit to the CO2supply using the ~1/4-inch or 6mm plastic tubing
(with installed HEPA filter) by attaching the tubing from the two-stage regulator
(or in-line regulator) to the matching CO2inlet on the rear of the incubator.
2. Use the tubing clips provided to eliminate CO2leaks.
HUMIDITY
TRAY

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New Brunswick Scientific User’s Guide
3
3 O OP
PE ER RA AT TI IO ON N
3.1 Control Panel
The control panel consists of two individual three-digit LED displays, and four
Function keys (see Figure 2 below).
Figure 2: Control Panel
Table 3: Function Keys
Key Symbol Key Name Function
∗Programming
Press this key to enter Programming mode, and to
set values in either display. Press this key
simultaneously with the Enter key to perform
AutoZero.
▼Down
In Programming mode, use this key to scroll down
through numbered values in the display. Press this
key simultaneously with the Enter key to work in
the Alarm system.
▲Up In Programming mode, use this key to scroll up
through numbered values in the display.
◄Enter
Press this key to save a new setpoint. Press this
key simultaneously with the Programming key to
perform AutoZero. Press this key simultaneously
with the Down key to work in the Alarm system.
NOTE:
If you accidentally press both the Up and Down keys simultaneously,
you will engage Engineering Mode: press the Programming key
immediately to exit.
CO2DISPLAY
(shown in normal
operation at factory
setpoint)
TEMPERATURE
DISPLAY
(shown in normal
operation at factory
setpoint)
FUNCTION KEYS

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M1310-0050 Model CO-150 CO2Incubator User’s Guide
3.2 Preparing for Operation
1. Remove the black protective cover from the CO2 sensor, and store it for use when
you clean the incubator. There is a storage holder at the rear of the unit (see
Figure 3 below).
Figure 3: Rear Panel
2. Ensure that the white porous sensor cover is in place.
3. Remove the silica gel dessicant sachet.
4. Using the power cord provided, connect the incubator to a grounded power
supply. The display will illuminate immediately.
NOTE:
The incubator does not contain a main power switch, and will turn on
immediately when connected to a power source. If the power
receptacle has a switch, make certain the power receptacle is switched
on. When the incubator turns on, the decimal points on the display for
Temperature and CO2 flash on and off to signify that the Alarm System
is not armed.
5. Turn on the gas supply with the pressure set to 5 psi or 0.35 bar when the
incubator is at the programmed temperature.
NOTE:
Be certain to check for leaks in the CO2connections to avoid depleting
your CO2gas supply. This can be accomplished using a solution of
soapy water applied to each fitting and checking for bubbles. If any
bubbles are noted, readjust the fitting.
6. The chamber setpoints are pre-programmed at 37.0°C and 5.0% CO2.
7. If you are not planning to humidify the incubator, leave the humidity tray in place
and skip to Step 4 in Section 3.3 below.
FUSE
HOLDER
POWER
INLET