NuAire NU-475-300 Manual

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CELLGARD ES Energy Saver Class II,
Type A2 Laminar Flow
Biological Safety Cabinet
Models
NU-475-300/400/500/600
Bench/Console
Operation & Maintenance Manual
January, 2011
Revision 1
(Series 1 & Higher)
Manufactured by:
NuAire, Inc.
2100 Fernbrook Lane
Plymouth, Minnesota USA 55447
Toll Free: 1-800-328-3352
In MN: 763-553-1270
Fax: [phone]
NU-475-400/500/600 Only

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Congratulations!
You have just purchased one of the finest Laminar Flow Biological Safety Cabinets available. With proper care,
maintenance (certification), and laboratory procedure, this cabinet will give you years of product and personnel protection
from particulate contaminants as prescribed in NSF/ANSI 49. Please read this manual carefully to familiarize you with
proper installation, maintenance and operation of the cabinet. Other reference and guideline materials are available
through the following web sites.
www.hc-sc.gc.ca
www.cdc.gov/od/ohs/
www.absa.org
www.absa-canada.org
www.ebsa.be
www.inspection.gc.ca
www.who.int
www.biosafety.be
www.hse.gov.uk
www.nsf.org
www.cetainternational.org
www.osha.gov/dts/osta/
www.nuaire.com

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ABOUT THIS OPERATION & MAINTENANCE MANUAL
The information contained in this manual is intended to reflect our current production standard configuration model along
with the more frequently purchased options. Any unique additions/modifications/shop drawings are appended in the back
flap of this manual, along with any modifications and/or additions to procedures as outlined in this manual. A copy of the
original factory test report is also appended to this manual. In case this manual and/or test report is lost or misplaced,
NuAire retains a copy in our files. A replacement copy can be obtained by calling or writing NuAire, Inc. stating the
model number and serial number and a brief description of the information desired.

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CELLGARD ES Energy Saver Class II, Type A2
Laminar Flow Biological Safety Cabinet
Models NU-475-300/400/500/600
Operation & Maintenance Manual
TABLE OF CONTENTS
Section No. 1..........................................................General Information
Section No. 2..........................................................Models & Features
Section No. 3..........................................................Warranty
Section No. 4..........................................................Shipments
Section No. 5..........................................................Installation Instructions
5.1 ..........................................................................Location
5.2 ..........................................................................Set-up Instructions
5.3 ..........................................................................Certification Testing Methods and Equipment
Section No. 6..........................................................Operating the NU-475
6.1 ..........................................................................Operator Controls & Indicators
6.2 ..........................................................................Operating Guidelines
6.3 ..........................................................................Operating Sequence
6.4 ..........................................................................Ergonomics
6.5 ..........................................................................Cleaning Procedure
6.6 ..........................................................................Hazardous Drug Decontamination Procedure
Section No. 7..........................................................General Maintenance
7.1 ..........................................................................Decontamination
7.2 ..........................................................................Fluorescent Lamp Bulb Replacement
7.3 ..........................................................................HEPA Filter/Motor Replacement
7.4 ..........................................................................Sliding Window Replacement & Adjustment
7.5 ..........................................................................Airflow Calibration
7.6 ..........................................................................HEPA Filter Leak Test
7.7 ..........................................................................Airflow Smoke Pattern Test
7.8 ..........................................................................Site Installation Assessment Tests
7.9 ..........................................................................Cleanliness Classification Test for Pharmacy Application
Section No. 8..........................................................Error Indicators & Troubleshooting
Section No. 9..........................................................Remote Contacts
Section No. 10........................................................Optional Equipment
10.1 ........................................................................Ultraviolet Light
Section No. 11........................................................Electrical/Environmental Requirements
Section No. 12........................................................Disposal and Recycle
MANUAL DRAWINGS
ACD-14251.....................................NU-475 Airflow Schematic
BCD-14243.....................................NU-475-300 Specification Drawing
BCD-14244.....................................NU-475-400 Specification Drawing
BCD-14245.....................................NU-475-500 Specification Drawing
BCD-14246.....................................NU-475-600 Specification Drawing
BCD-11815.....................................Drain Valve Installation
BCD-11816.....................................Front Panel
ASSEMBLY DRAWINGS
BCD-05147.....................................Base Stand Assembly
BCD-05146.....................................Base Stand Storage Cabinet Assembly
BCD-11817.....................................Control Center & Front Decorative Panel Assembly
BCD-11686.....................................Sliding Window Assembly & Adjustment
BCD-11819.....................................Base Cabinet Assembly
BCD-12271.....................................Window Assembly Hinge Operation
ELECTRICAL SCHEMATICS
BCD-14240.....................................NU-475-300/400/500/600 Electrical Schematic

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CELLGARD ES Energy Saver Class II, Type A2
Laminar Flow Biological Safety Cabinet
Models
NU-475-300/400/500/600
MANUFACTURED BY:
NuAire, Inc. - Plymouth, Minnesota, U.S.A.
1.0 General Information
1.1 Description
The CELLGARD ES Model NU-475 Laminar Flow Biological Safety Cabinet (LFBSC) is a bench/table top
model, optionally available with a base support stand, for operation as a console model. The CELLGARD ES model
NU-475 utilizes an Energy Saver DC ECM motor optimally determined forward curved fan for each model size/width to
maximize both energy efficiency and filter loading capacity. The Energy Saver ECM motor is controlled via a solid-state
DC motor controller that provides automatic compensation (constant volume control) for both filter loading and line
voltage variances.
The Laminar Flow Biological Safety Cabinet, (LFBSC) is a product resulting from the development of the
"laminar flow" principle and the application of environmental controls as required in the field of biological research or
chemical containment. The LFBSC, when used with proper technique, is an effective laboratory aid in obtaining the
optimum control over product quality while reducing the potential for exposure of both product and personnel to airborne
biological or particulate chemical agents in low to moderate risk-hazard research and drug preparation or product
operations, as prescribed by the Center for Disease Control (CDC) Atlanta, Georgia.
The NU-475 bench LFBSC is known as a Class II, Type A2 Biological Safety Cabinet. This is possible since
NuAire's cabinet conforms to the following requirements:
1. Maintains inflow velocity of 100 LFPM (.51m/s) through the work access opening.
2. Has HEPA filtered downflow air that is mixed with the inflow air from a common exhaust plenum.
3. Exhaust airflow can either be room re-circulated or exhausted outside using a canopy exhaust transition.
4. Has all biologically contaminated ducts and plenums under negative pressure or surrounded by negative
pressure. (Type A1 permits positive pressure contaminated ducts and plenums).
Cabinets used for work with minute quantities of volatile toxic chemicals and traces amounts of
radionuclides required as an adjunct to microbiological studies must be exhausted through properly
functioning exhaust canopies.

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1.2 Safety Instructions
These safety instructions describe the safety features of the CELLGARD ES Model NU-475 LFBSC.
The safety cabinet has been manufactured using the latest technological developments and has been thoroughly
tested before delivery. It may, however, present potential hazards if it is not used according to the intended
purpose or outside of operating parameters. Therefore, the following procedures must always be observed:
The safety cabinet must be operated only by trained and authorized personnel.
For any operation of this unit, the operator must prepare clear and concise written instructions for
operating and cleaning, utilizing applicable safety data sheets, plant hygiene guidelines, and technical
regulations, in particular.
owhich decontamination measures are to be applied for the cabinet and accessories,
owhich protective measures apply while specific agents are used,
owhich measures are to be taken in the case of an accident.
Repairs to the device must be carried out only by trained and authorized expert personnel.
Keep these operating instructions close to the unit so that safety instructions and important information
are always accessible.
Should you encounter problems that are not detailed adequately in the operating instructions, please
contact your NuAire Representative of NuAire technical Services.
1.3 Explanation of Symbols
NOTE:
CAUTION used without the safety alert symbol
indicates a potentially hazardous situation which, if
not avoided, may result in property damage.
Potential electrical hazard, only qualified person
to access.
Used for important information.
Biohazard
Ground, Earth
CAUTION:

Lead Free Chemical Hazard
Flammable Hazard
Hazardous Gases! Personal
Protection Equipment Required.
Safety alert symbol indicates a potentially
hazardous situation which, if not avoided, could
result in death of serious injury.
Safety alert symbol indicates a potentially hazardous
situation which, if not avoided, may result in minor
or moderate injury.
WARNING
!
CAUTION
!

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2.0 Models & Features
The model NU-475, Class II, Type A2 Laminar Flow Biological Safety Cabinet is manufactured in four sizes:
3 ft., 4 ft., 5 ft., and 6 ft.

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