NUVE EC 160 User manual

NÜVE SANAYİ MALZEMELERİ İMALAT VE TİCARET A.Ş.
EC 160
CO2INCUBATOR
USER’S MANUAL
Z14.K25 281 Rev. No: 10 Rev. Date: 01/2018

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Dear Nüve User,
We would like to take this opportunity to thank you for preferring this Nüve product.
Please read the operating instructions carefully and keep them handy for future
reference.
Please detain the packing material until you see that the unit is in good condition and it
is operating properly. If an external or internal damage is observed, contact the
transportation company immediately and report the damage. According to ICC
regulations, this responsibility belongs to the customer.
While you are operating the instrument please;
1. obey all warning labels,
2. do not remove warning labels,
3. do not operate damaged instrument,
4. do not operate instrument with a damaged cable,
5. do not move instrument during operation.
In case of a problem contact your Nüve agent for an authorized service or maintenance.
The validity of the guarantee is subject to compliance with the instructions and
precautions described in this manual.
Nüve reserves the right to improve or change the design of its products without any
obligation to modify previously manufactured products.
Information contained in this document is the property of Nüve. It may not be duplicated
or distributed without our permission.
NÜVE
SANAYİ MALZEMELERİ
İMALAT VE TİCARET A.Ş.
Saracalar Mahallesi, Saracalar Kümeevleri No:4/2 Akyurt 06750
Ankara / TURKEY
Tel : [phone] (pbx)
Fax : [phone]
Sales : [email protected]
Technical Service: nuvese[email protected]

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WARRANTY CERTIFICATE
1. Nüve warrants that the equipment delivered is free from defects in material and
workmanship. This warranty is given for a period of two years. The warranty period
begins from the delivery date.
2. Warranty does not apply to parts normally consumed during operation or general
maintenance or any adjustments described in the operating instructions provided
with the instrument.
3. Nüve does not accept any liability in case where the goods are not used in
accordance with their proper intent.
4. The warranty may not be claimed for damages incurred during the shipment, for
damages resulting from improper handling or use, abuse, fire, liquid spillage,
tampering or unauthorized repairs by any persons, use of defective or incompatible
accessories, exposure to abnormally corrosive conditions, use of the product in non-
standard environmental conditions, including but not limited to failure to meet
requirements of ambient temperature, lubrication, humidity or magnetic field
influences, from the defects in maintenance, negligence, bad functioning of auxiliary
equipment, in the case of force majeure or accident and incorrect power supply.
5. Any injury, loss or damage caused; due to a failure resulting from negligence of the
instructions given in this manual; is beyond the scope of the warranty conditions.

BEFORE OPERATING THE INSTRUMENT THIS MANUAL SHOULD BE
READ CAREFULLY.

THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION
OF THE INSTRUCTIONS AND PRECAUTIONS DESCRIBED IN THIS
MANUAL.
INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF
NÜVE. IT MAY NOT BE DUPLICATED OR DISTRIBUTED WITHOUT
PERMISSION.
PLEASE REGISTER ONLINE TO VALIDATE YOUR WARRANTY:
To register your warranty online, please visit our webpage www.nuve.com.tr and fill in
the “Warranty Registration Form”.

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CONTENT
Page
CONTENT 4
1. INTRODUCTION 6
1.1. USE AND FUNCTION 6
2. TECHNICAL SPECIFICATIONS 8
2.1. TECHNICAL SPECIFICATIONS TABLE 8
2.2. ACCESSORIES 8
2.2.1. FACTORY FITTED ACCESSORIES 8
2.2.2. OPTIONAL ACCESSORIES 8
3. PRECAUTIONS AND LIMITATIONS ON USE 9
4. SYMBOLS AND LABELS 10
5. INSTALLATION 10
5.1. ENVIRONMENTAL CONDITIONS 10
5.2. HANDLING AND TRANSPORTATION 11
5.3. UNPACKING 11
5.4. MAINS SUPPLY 11
5.5. POSITIONING 11
5.6. GENERAL PRESENTATION 12
5.7. CONTROL PANEL 15
5.8. PRIOR TO OPERATION 15
5.8.1. MOUNTING THE SHELVES AND HUMIDITY TRAY 15
5.8.2. MOUNTING BIOLOGICAL FILTERS 16
5.8.3. CO2 TANK CONNECTION AND REPLACEMENT 16
6. OPERATING PRINCIPLES 17
6.1. SWITCHING ON 17
6.2. OPERATING MODE AND MENU 18
6.2.1. OPERATING MODE 18
6.2.2. SET BUTTON 20
6.2.3. GRAPHIC SCREEN BUTTON 20

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6.2.4. HELP BUTTON 21
6.2.5. MENU BUTTON 21
6.3. DISINFECTION 22
6.3.1. NüveDis™ DISINFECTION AT 90⁰C22
6.3.2. UV DISINFECTION WITH nUVeRay™ 24
6.4. SERVICE 26
6.5. SETTINGS 26
6.5.1. SECURITY SETTINGS 26
6.5.2. MEMORY 27
7. PERIODIC MAINTENANCE AND CLEANING 27
7.1. PERIODIC MAINTENANCE 27
7.2. CLEANING 27
8. DISPOSAL MANAGEMENT CONCEPT 28
9. TROUBLESHOOTING 28
9.1. ERROR CODES 28
9.2. FUSE REPLACEMENT 29
10. OPTIONS 29
10.1. AlerText™ GSM ALARM MODULE 29
10.1.1. CONNECTING THE GSM MODULE TO THE DEVICE 29
10.2. CO2CHANGEOVER UNIT 30
10.3. NuveCell™ SOFTWARE 31
10.4. Ø25 mm CABLE INPUT 31
10.5. NuveWarn™ REMOTE ALARM SYSTEM 31
10.5.1. MOUNTING THE REMOTE ALARM KIT 32
10.6. nUVeRay™ UV DISINFECTION KIT 32
11. ELECTRICAL CIRCUIT DIAGRAM 33

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1. INTRODUCTION
1.1. USE AND FUNCTION
EC 160 CO2Incubator is especially designed for the applications in microbiology,
hematology, vitro fertilization centers, oncology, dermatology, infection diseases,
neurology, geriatrics, veterinary, neurobiology, pharmaceutical industry, dentistry,
toxicology and internal diseases and other general and special applications.
EC 160 CO2Incubator maintains temperatures between ambient temperature + 7°C and
50°C and keep the temperature stable within the given tolerances. CO2concentration
could be set between the values of 0.0 % and 20.0 %. The incubator will automatically
start to operate according to its set values. The temperature is controlled with PID
controlled microprocessor system as CO2is controlled with proportional system.
Adjustable safety thermostat offers an additional safety for operations.
Advanced microprocessor system enables homogenous and stable temperature and
CO2distribution in the chamber. Auto-Zero system validates CO2measurement every
12 minutes. Also another temperature sensor consists for reading ambient temperature.
The volume of EC 160 CO2Incubator’s chamber is 160 liters and is manufactured as in
one piece deep drawn stainless steel without any joint in order to prevent contamination.
Rounded edges in chamber and removable shelves allow user to clean the chamber
easily.
LCD technology is used for the display and display interface can be used in three
different languages which are Turkish, English and French.
EC 160 is equipped with an advanced alarm system. Audial and visual alarms are used
to warn the user when the door is open, the chamber temperature and CO2is out of the
set values, the temperature sensor is broken, a problem is occurred in microprocessor
control system or during power cut. Remote alarm with battery supply is provided with
the incubator. NC contact part is also available in order to use with special alarm
systems.
EC 160 CO2Incubator is designed to maintain the following conditions inside the
chamber:
Sterile operating environment,
Stabile temperature,
Enriched CO2operating environment,
High humidity level.
Sterile operating environment
The chamber is isolated from the external environment by the help of the gasket.
Therefore; inside of the chamber maintains its sterility during any operation.

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There are 3 different biological filters in the incubator in order to keep the chamber
sterile.
CO2gas passes through 0,22 µm biological filter before it reaches into the chamber.
During the Auto-Zero, ambient air passes through 0,22 µm biological filter and then it
goes to the Infrared Sensor (IR).
The air inside the chamber passes through 0,22 µm biological filter continuously
before it goes to the chamber again.
Stabile temperature
Chamber temperature is kept stable and homogeneous by the help of air jacket around
the chamber. Heaters which help to maintain the stable and uniform temperature inside
the chamber is located in right, left and bottom of the air jacket. The glass internal door
is heated by means of the heater inside the outer door. PID controlled microprocessor
control system guarantees precise temperature control and safety thermostat provides
secure operating environment.
Enriched CO2operating environment
The main reason of the incubator is to provide enriched CO2environment. CO2
decomposes inside the media; therefore, it prevents the alkalization which is harmful for
the cells.
The pH of the culture media is kept constant by sodium bicarbonate. The chemical
reaction of CO2and sodium bicarbonate can be represented as:
CO2+ H2O <=> H++ HCO3-<=> 2 H++ CO32-
The continuous chemical reaction by maintaining a constant level of CO2gas assures
the proper pH for the growth of cells.
Auto-Zero System
Validation of infrared CO2sensor is done by the means of Auto-Zero System which is
given as standard feature of EC 160. The filtered ambient air, which is supposed to have
0% CO2, is taken to IR sensor automatically in every 12 minutes and IR sensor is
calibrated 120 times within a day without waiting its calibration. When the Auto-Zero
system is active, it is seen “z” next to the CO2on the display.
High Humidity Level
EC 160 Incubator provides an environment which is enriched with the mixture of water
and gas in order to prevent the evaporation of the culture media and keeps the pH as in
desired level. High humidity level is provided with the help of humidity tray which is
located bottom of the chamber. Humidity tray contacts with the heated bottom surface;
therefore, evaporation of water occurs naturally and provides 95% ± 5% humidity at
37.0°C. High relative humidity prevents desiccation of the media and maintains the
correct osmolality.

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EC 160 CO2 Incubator is designed and manufactured in accordance with
international directives EN 61010-1, EN 61326 and DIN 12880. If the warnings
mentioned in this manual are not considered, NUVE will not be responsible from
their results.
2. TECHNICAL SPECIFICATIONS
2.1. TECHNICAL SPECIFICATIONS TABLE
TECHNICAL SPECIFICATIONS
EC 160
Temperature Range
Ambient Temperature +7°C / 50°C
Temperature Sensor
Pt-100
Control System
N-Smart™Microprocessor Control System
Temperature Sensitivity
0.1°C
Temperature Distribution (at 37°C)
± 0.3°C
Temperature fluctuation (at 37°C)
±0.1°C
Disinfection temperature
90°C
CO2Setting Range
%0.0 - 20.0
CO2Set and Display Sensitivity
%0.1
CO2Sensor
IR Sensor
CO2Sensor / Auto-Zero
Standard –In every 12 minutes
Safety System
Gas expansion thermostat
Chamber Capacity
160 liters
Number of Shelves (std./max)
3 / 8
Power Consumption
1160 W
Power Supply
230 V, 50/60 Hz
Internal Material
Stainless Steel
External Material
Epoxy polyester coated steel
Internal Dimensions (W x D x H) (mm)
500 x 460 x 700
External Dimensions (W x D x H) (mm)
917 x 722 x 905
2.2. ACCESSORIES
2.2.1. FACTORY FITTED ACCESSORIES
EC 160H Cable port (25 mm)
EC 160W N-Smart™Data monitoring software and RS 232 port
2.2.2. OPTIONAL ACCESSORIES
A 08 142 AlerText™ GSM alarm module (SIM card is not included.)
A 08 143 nUVeRay™ UV light sterilization kit
A 08 144 NuveClean™ Decontamination kit for humidity tray (20 doses)
K 13 009 NuveWarn™ Remote alarm system with 10 m cable
A 08 146 Stacking Adapter
A 08 147 Support Frame

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A 08 148 Roller Base
A 08 149 Gas Changeover Unit for two CO2gas tanks (Tanks not included)
F 06 003 0,22 µm biological filter (Spare)
R 11 007 Single Stage Regulator for CO2gas tank
R 01 154 Stainless steel shelf

A 08 142 and K 13 009 cannot be used at the same time.
3. PRECAUTIONS AND LIMITATIONS ON USE
The user shall pay attention to the following:
Do not operate the instrument for purposes other than its main purpose.
The instrument should only be used by authorized and trained staff after the
instruction manual has been read carefully. Only authorized technical staff can
handle the product in case of a failure.
Electricity line should be suitable for the power of device and correctly grounded
power supply should be used.
Only original spare parts and original accessories supplied by Nüve should be used.
The set temperature should not destroy the structure of the samples without user’s
notice.
The boiling points of the samples should be higher than the set temperature.
The freezing points of the samples are lower than the set the temperature.
The samples which may liquefy and expand should not be in a sealed container.
Liquids which may expand during heating should not overflow from their containers.
The safety thermostat should be adjusted to the temperature higher than the set
temperature.
There should not be any material in the chamber that can damage to the device.
The materials which will be subject to heating should not be combustible, explosive,
heat susceptible, flammable, adhesive and fusible.
Ensure that the vapors and gases generated during the operation are not harmful to
human health and flammable or explosive.
CO2is a toxic gas. If the door of EC 160 is kept opened for long periods, the
CO2 gas in the chamber is diffused to the laboratory. In that case, ventilate the
laboratory.

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4. SYMBOLS AND LABELS
Symbol in the operating instructions:
Attention, general hazard area.
This symbol refers to safety relevant warnings and indicates possibly
dangerous situations. The non-adherence to these warnings can lead to
material damage and injury to personal.
Notified Body:
KIWA
Belgelendirme Hizmetleri A.Ş.
(İTOSB) İstanbul Tuzla Organize Sanayi Bölgesi Tepeören Mevkii
34597 Tuzla- İstanbul / TURKEY

Symbol in the operating instructions:
This symbol refers to important circumstances.
Type B protection.
Before operating the instrument this manual should be read carefully.
Labels on the product:
Earthed Wall Sockets
Fuses (2 x 1A)
Fuses (1 x 5A)
UV Lamp
5. INSTALLATION
5.1. ENVIRONMENTAL CONDITIONS
The CO2İncubator is designed to operate safely under the following conditions:

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Indoor use only
Ambient temperature: 5°C to 40°C
Maximum relative humidity for temperature up to 31°C: 80%
Maximum altitude: 2000 m
Temperature for maximum performance: 15°C / 25°C
5.2. HANDLING AND TRANSPORTATION
All handling and transportation must be carried out by using proper equipment and
experienced staff. The instrument must be supported underneath and never be turned
upside down.
5.3. UNPACKING
Remove the cardboard box packing and the second nylon wrapping around the
instrument. Ensure that no damage has occurred during transportation. The below
mentioned are provided with the instrument, please check them;
1 pc. user’s manual and warranty
2 pcs. biological filter
3 pcs. shelves
2 pcs. of shelves’ support
1 pc. shelves’ stick
Humidity tray
1 pc. power cable
2.5 meters tube for CO2connection
5.4. MAINS SUPPLY
The CO2Incubator requires 230 V, 50/60 Hz.
Please make sure that the supplied mains matches the required power ratings which are
written on the name of plate of the instrument located at the back of the instrument.
Always plug-in the instrument to correctly grounded sockets.
A supply fitted with a circuit breaker should be used for protection against
indirect contact in case of isolation fault.
5.5. POSITIONING
Check that the positioning is suitable for the users.
Check that the instrument is stable on its four pedestals.
Check that the user will be able to follow up the operation even when he deals with
something else.
Check that the positioning of the device prevents interference with other equipment
in the near surrounding.
The bench where the instrument is positioned should be resistant to the weight of the
instrument and vibration free.
Leave at least 30 cm space on the left, right and back side of the instrument.

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Positioning of EC 160 should be away from windows and walls. The instrument
should be in a place around which the air can ventilate easily.
EC 160 should not be close to the window and exposed to sun light.
Protect the device from direct ventilation.
EC 160 should not be sited on thoroughfares or in line with doorways or opening
windows.
Doors of the room should not be opened frequently.
If there is laminar flow cabinet in the room, it should not blow towards to EC 160.
The air ventilation in the room should be minimum.
If there is air condition in the room, it should be on during night and day. If air
condition is on during day time, do not switch off air condition during night. Or it
should be off all the time.
The room temperature should be stable and it should be maximum 30⁰C.
5.6. GENERAL PRESENTATION
Figure 1
1- CONTROL PANEL
2- CHAMBER GASKET
3- BIOLOGICAL FILTER
4- SHELF
5- GLASS DOOR
6- UV SOCKET
7- DOOR SWITCH
8- HUMIDITY TRAY

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Figure 2
9- CO2MEASUREMENT ACCESS PORT
10- AMBIENT TEMPERATURE SENSOR
11- REMOTE ALARM PORT
12- CO2 TANK PORT
13- BIOLOGICAL FILTER
14- SAFETY THERMOSTAT
15- POWER SWITCH
16- POWER SUPPLY AND FUSES

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1- Control Panel: Control panel includes LC display in order to control the device and
set the desired values by using 4 different keys.
2- Chamber Gasket: The silicon gasket prevents the leakage.
3- Biological Filter: The filter is used to generate a sterile environment inside the
chamber.
4- Shelf: Stainless steel shelf is easy to remove.
5- Glass Door: The door helps the user to observe the samples inside the chamber.
6- UV Socket: nUVeRay™ is connected to this socket when the user wants to use the
UV Disinfection program.
7- Door Switch: With the help of the door switch, visible and audible alarm activates
when the glass door is left open.
8- Humidity Tray: This humidity tray is used to generate humidity by means of the
distilled water inside the tray.
9- CO2Measurement Access Port: User could use the port in order to connect an
external measurement device and measure the CO2percentage inside the chamber.
10-Ambient Temperature Sensor: Measure instant ambient temperature by Pt-100
sensor.
11-Remote Alarm Port: When optional NuveWarn™ remote alarm system is
connected to this port, the user is notified in case of alarms. Optional AlerText™
GSM alarm module is also connected to this port.
12-CO2Tank Port: CO2connection is made via this input.
13-Biological Filter: This filter is used to maintain a sterile environment inside the
chamber.
14-Safety Thermostat: Heating process is stopped automatically by the safety
thermostat in case of uncontrolled overheating.
15-Power Switch: This switch is used to power on or off the device.
16-Power Supply and Fuses: In order to give energy to the device this power supply
input is used. Fuses protect the incubator from instantaneous voltage fluctuation in
mains supply.

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5.7. CONTROL PANEL
Figure 3
F1, F2, F3 and F4 buttons are named with the first letter of function and their functions
correspond to the commands on the display.
5.8. PRIOR TO OPERATION
5.8.1. MOUNTING THE SHELVES AND HUMIDITY TRAY
Put the shelves’ supports inside the chamber as it is shown in Figure 4.
Figure 4
After placing the humidity tray on the shelves’ support, put the shelves as in Figure 5.
F1
F2
F3
F4

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
Distilled water must be used in the humidity tray.
Figure 5
5.8.2. MOUNTING BIOLOGICAL FILTERS
Mounting the biological filter inside the chamber
Mount the biological filter into its place as in Figure 6.
Figure 6
Mounting the biological filter outside of the chamber
Mount the biological filter into “FILTER” port located at the back of the instrument as
shown in Figure 2.
5.8.3. CO2 TANK CONNECTION AND REPLACEMENT
Place CO2upright and take necessary precautions for not falling over the tank.
After connected the regulator to the CO2tank, follow the steps bellow:

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Connect the tube between the CO2tank’s regulator and the CO2tank port of the
incubator (Figure 2).
Check the connection and be sure there is no leakage.
Turn on the CO2tank.
Observe the CO2tank’s pressure.
Set the regulator to 2 bars (6-7 liter/minute).
The tube used between CO2tank and the instrument should resist to 5 bars.
Tighten the connection of the tube with clamps and ensure that there is no
leakage.
Please prevent the CO2gas tank from sun lights and heat. The distance
between tank and a heat source must be at least 0.5 meters. The gas is
inside the CO2tank is heavier than the air and has risks for human’s heath.
Figure 7
6. OPERATING PRINCIPLES
6.1. SWITCHING ON
Plug-in the instrument to correctly grounded sockets.
Turn on the device by power switch which is at the back of the incubator and see the
LED of power switch is on.
See that the control and the display panel are activated.
Learn the function of the control and display panel (See Section 5.7).
The system controls itself for 60 seconds after it is energized.

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Ensure that the safety thermostat is adjusted to a value higher than the set
temperature.

Prior to operation ensure that no substance has been left in the chamber.
At most 70% of the surface area of the shelves should be loaded in order to
obtain a uniform temperature distribution.
6.2. OPERATING MODE AND MENU
6.2.1. OPERATING MODE
NUVE logo appears on the screen for 1-2
minutes for control process This screen is
displayed until control process is done.
Incubator starts to operate
automatically according to the set
values. On the operating mode
chamber temperature, CO2value
and set temperature and CO2 value
are displayed.
NOTIFICATION AREA

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
The menu password for EC 160 CO2Incubators is 2112. It is suggested to
change it for your security.

Door temperature value aims for avoiding influence of ambient temperature to
chamber temperature distribution. Door set temperature equals to chamber
set temperature value + door temperature difference.
Lock icon appears on the right
bottom corner if the device is
locked. Press that icon to reach the
keypad for unlocking the device.
Also sensor values button appears
at the left bottom corner.
Security screen contains user
defined password. With the correct
password combination menu screen
opens directly.

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6.2.2. SET BUTTON

When the ambient temperature is cold in winter, it is suggested to set the door
temperature difference value as +2.0.
6.2.3. GRAPHIC SCREEN BUTTON
Values of chamber temperature,
temperature alarm, CO2, CO2 alarm
and door temperature difference
are shown in this screen. Pressing
up and down button you can
increase or decrease value of
parameter after choosing with
ENTER (F4) button.
Chamber temperature and CO2
value are shown graphical and two
different adjustable scales with
respect to time. Temperature and
CO2 scales can be adjusted by
pushing select button.