NUVE EN 300 User manual

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NÜVE SANAYİ MALZEMELERİ İMALAT VE TİCARET A.Ş.
EN 300 - EN 400 - EN 400P
EN 500 –EN 500P
INCUBATORS
USER’S MANUAL
Z14 K25 231 Rev.No:06 Rev.Date: 10/2017

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MANUFACTURER :
NÜVE SANAYİ MALZEMELERİ
İMALAT VE TİCARET A.Ş.
Saracalar Mahallesi, Saracalar Kümeevleri No:4/2 Akyurt 06750
ANKARA - TURKEY
TEL: +(90) 312 399 28 30 (pbx)
FAX: +(90) 312 399 21 97
E-mail:[e-mail]
WARRANTY CERTIFICATE
1. Nüve warrants that the equipment delivered is free from defects during material and
workmanship. This warranty is provided for a period of two years. The warranty period
begins from the delivery date.
2. Warranty does not apply to parts normally consumed during operation or general
maintenance or any adjustments described in the operating instructions provided with the
equipment.
3. Nüve does not accept any liability in the case where the goods are not used in accordance
with their proper intent.
4. The warranty may not be claimed for damages occurred during the shipment, for damages
resulting from improper handling or use, the defects in maintenance, negligence, bad
functioning of auxiliary equipment, in the case of force majeure or accident and incorrect
power supply.
5. In the event of failure, Nüve shall be under no liability for any injury, or any loss or damage
as the result of the failure other than the guarantee conditions.
BEFORE OPERATING THE INSTRUMENT THIS MANUAL SHOULD BE READ
CAREFULLY.
THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE
INSTRUCTIONS AND PRECAUTIONS DESCRIBED IN THIS MANUAL.
INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF NÜVE. IT MAY
NOT BE DUPLICATED OR DISTRIBUTED WITHOUT PERMISSION.

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Dear Nüve User,
We would like to take this opportunity to thank you for preferring this Nüve product. Please read
the operating instructions carefully and keep them handy for future reference.
Please detain the packing material until you see that the unit is in good condition and it is operating
properly. If an external or internal damage is observed, contact the transportation company
immediately and report the damage. According to ICC regulations, this responsibility belongs to the
customer.
While you are operating the instrument please;
•obey all the warning labels,
•do not remove the warning labels,
•do not operate damaged instrument,
•do not operate the instrument with a damaged cable,
•do not move the instrument during operation.
In case of a problem contact your Nüve agent for an authorized service or maintenance.
The validity of the guarantee is subject to compliance with the instructions and precautions
described in this manual.
Nüve reserves the right to improve or change the design of its products without any obligation to
modify previously manufactured products.
Information contained in this document is the property of Nüve. It may not be duplicated or
distributed without its permission.
Lifetime of the device is 10 years.
PLEASE REGISTER ONLINE TO VALIDATE WARRANTY:
To register your warranty online, please visit our web page www.nuve.com.tr and fill in WARRANTY
REGISTRATION FORM.

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TABLE OF CONTENTS
SECTION 1 ......................................................................................................................................................4
INTRODUCTION..............................................................................................................................................4
1.1 USE AND FUNCTION .......................................................................................................................4
SECTION 2 ......................................................................................................................................................5
TECHNICAL SPECIFICATIONS.....................................................................................................................5
2.1 TECHNICAL SPECIFICATIONS TABLE...........................................................................................5
2.2 OPTIONAL ACCESSORIES.............................................................................................................5
2.3 GENERAL PRESENTATION............................................................................................................6
SECTION 3 ......................................................................................................................................................7
INSTALLATION PROCEDURE.......................................................................................................................7
3.1 LIFTING AND TRANSPORT .............................................................................................................7
3.2 UNPACKING .....................................................................................................................................7
3.3 POSITIONING...................................................................................................................................7
3.4 MAINS SUPPLY ................................................................................................................................8
3.5 PRIOR TO INCUBATION ..................................................................................................................8
SECTION 4 ......................................................................................................................................................8
OPERATING PRINCIPLE ...............................................................................................................................8
4.1 SWITCHING ON................................................................................................................................8
4.2 DISPLAY AND CONTROL PANEL....................................................................................................9
4.2.1 Explanations and Functions for Display and Control Panel.......................................................9
4.3 PREPARATION OF USER SETTINGS .......................................................................................... 10
4.3.1 oP Operator Menu Parameters............................................................................................... 11
4.4 PROGRAMMING SUMMARY ........................................................................................................ 11
4.5 COMPLETION OF THEWORK...................................................................................................... 13
SECTION 5 ....................................................................................................................................................14
PERIODIC MAINTENANCE AND CLEANING .............................................................................................14
5.1 PERIODIC MAINTENANCE........................................................................................................... 14
5.2 CLEANING ..................................................................................................................................... 14
SECTION 6 ....................................................................................................................................................14
DISPOSAL MANAGEMENT CONSEPT.......................................................................................................14
SECTION 7 ....................................................................................................................................................14
TROUBLESHOOTING ..................................................................................................................................14
7.1 ERROR CODES EXPLANATIONS................................................................................................. 15
SECTION 8 ...................................................................................................................................................16
ELECTRICAL CIRCUIT DIAGRAM ..............................................................................................................16
8.1 EN 300 ELECTRICAL CIRCUIT DIAGRAM.................................................................................... 16
8.2 EN 400 ELECTRICAL CIRCUIT DIAGRAM.................................................................................... 17
8.3 EN 500 ELECTRICAL CIRCUIT DIAGRAM.................................................................................... 18
8.4 EN 400P –500P ELECTRICAL CIRCUIT DIAGRAM..................................................................... 19
SECTION 9 ...................................................................................................................................................20
WARNING LABEL.........................................................................................................................................20

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SECTION 1
INTRODUCTION
1.1 USE AND FUNCTION
The EN 300, EN 400, EN 400 P, EN 500 and EN 500 P incubators are designed to incubate
samples in biological, medical and pharmaceutical laboratories and in many industrial control
laboratories.
They maintain drying and incubation temperatures between 5°C above the ambient temperature
and 80°C and keep the temperature stable within the given tolerances.
The EN series incubators provide homogeneous temperature distribution by means of the sheet
heaters placed onto three outer surfaces of the useful volume.
The incubator ensures reliable working conditions by the programmable microprocessor controlled
main PCB, which has a very high control accuracy. As an additional security feature, the safety
thermostat is also available.
Microprocessor control system will shut down the temperature sensor and in case of malfunctions
that may occur in the control system, alarm system will be activated and the user is warned visually
and audibly. The study data are recorded in the memory and can be transferred to external USB
memory. At the same time, unauthorized persons have been blocked permission to change
parameters with improved password menu.
The EN series incubators are manufactured according to the following standards,
EN 61010-1, EN 61010-2-010, EN 61000-6-3, EN 50419, EN 61326-1.
This device is in compliance with WEEE Regulation.

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SECTION 2
TECHNICAL SPECIFICATIONS
2.1 TECHNICAL SPECIFICATIONS TABLE
EN 300
EN 400
EN 400 P
EN 500
EN 500 P
Temperature Range
Ambient Temp + 5° C / 80 ° C
Temperature Sensor
Fe-Const
Control System
N-PrimeTM
Temperature Set and Display
Sensitivity
0.1° C
Temperature variation (up to 40
°C)/(40°C -80°C)
± 0.5° C / ±1°C
Temperature fluctuation
± 1 ° C
Timer
1 minute –99.9 hours + hold
Program delay time
1 minute –99.9 hours
Safety thermostat
Gas expansion thermostat (0 - 90°C)
Useful Volume, liters
22
44
42
120
110
Number of Shelves ( Standard/
Max.)
2/6
2/7
2/7
2/10
2/10
Power Consumption
100 W
200 W
238 W
350 W
388 W
Power Supply
230 V, 50 / 60 Hz
Memory
3000 data*
Internal Material
Electro- acid
Coated
Aluminum
Electro- acid
Coated
Aluminum
Steel
Stainless
Electro- acid
Coated
aluminum
Stainless
Steel
External Material
Epoxy - Polyester Painted Steel
Internal Dimension (WxDxH)mm
300x240x300
420x320x360
420x280x360
500x490x500
500x450x500
External Dimension (WxDxH)mm
555x380x460
705x475x540
700x525x540
780x635x675
790x675x670
Packing Dimension (WxDxH)mm
640x470x640
790x570x760
790x570x760
870x740x860
870x740x860
Net/Packed Weight
26 / 31
39 / 45
39 / 45
60 / 70
66 / 74
*Shows the number of lines for each work done. Temperature, time and if there is error code
consist of 1 line.
**Differently from EN 400 and EN 500, inner sides of EN 400P and EN 500P are made of stainless
steel and their heaters are circular heaters which provide the homogeneity of the temperature by
the circulation fans placed on their centers.
2.2 OPTIONAL ACCESSORIES
R 01 014 Mesh type shelf for EN 300
R 01 136 Mesh type shelf for EN 400
R 01 126 Mesh type shelf for EN 400P
R 01 135 Mesh type shelf for EN 500
R 01 111 Mesh type shelf for EN 500P
K 23 031 Shelf carrier for EN 300

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K 23 047 Shelf carrier for EN 400
K 23 048 Shelf carrier for EN 400P
K 23 046 Shelf carrier for EN 500
K 23 040 Shelf carrier for EN 500P
NOTE: 2 pcs. shelf carrier should be ordered for each shelf.
2.3 GENERAL PRESENTATION
1
Display and control panel
6
Door
2
Safety thermostat adjusting button
7
Ventilation hole
3
On / Off switch
8
Glass door
4
Shelf
9
Glass door handle
5
Chamber gasket
10
Input USB

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SECTION 3
INSTALLATION PROCEDURE
3.1 LIFTING AND TRANSPORT
Because of the heavy weight of the incubator, all lifting and transport must be carried out using
proper handling equipment. The incubator must be supported from underneath and never turned
over.
3.2 UNPACKING
Remove the packing cardboard box and the second nylon packing around the incubator. The
below written are provided with the equipment, please check them;
User’s manual
1 piece of warranty certification
2 piece of shelves
4 piece of shelf carriers
1 piece of power cable
Check that no damage has occurred during transport.
3.3 POSITIONING
Lift the incubator underneath and carry it carefully to its place.
Balance the incubator on four pedestals. If necessary, provide stable standing by adjusting the
pedestal heights.
Place the shelf carriers and then the shelves.
Check the followings,
The proposed site is suitable for the user,
The operator can follow up the even he deals with something else.
The incubator does not occupy the utilisation space of others or does not damage them.
Leave at least 20 cm. free space between the equipment and wall.
ATTENTION !!!
Please pay special attention to the followings,
Indoor use only
Temperature from 5°C to 40°C
Maximum relative humidity of 80% for temperature up to 22°C,
Maximum altitude: 2000 m.
The maximum performance is obtained between 15°C and 25°C.
At most 70% of the surface area of the shelves should be used in order to obtain a uniform
temperature distribution.

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3.4 MAINS SUPPLY
The incubator requires 230 V, 50 Hz.
Please make sure that the supplied mains matches the required power ratings.
Always plug-in the ıncubator to correctly grounded sockets.
3.5 PRIOR TO INCUBATION
Plug the instrument in to a grounded socket.
Check the followings,
Make sure that the safety thermostat is adjusted to the temperatures which are higher than the
set temperature.
If it is necessary the ventilation hole is open to discharge the gases and the vapours which
occur during incubation.
Liquids are not heated in sealed containers.
The boiling points of the samples are higher than the set temperature.
Liquids which may expand during heating do not overflow from their containers.
The vapours and gases which are generated during the operation are not harmful to humans
or flammable or explosive.
The set temperature does not destroy the structure of the samples.
Plug the power cable into a grounded socket.
The safety thermostat set value should always be set to a value which higher than the
working temperature.
NOTE :
Never use explosive, flammable, acidic or toxic liquids. Read carefully the functions
of the control panel.
SECTION 4
OPERATING PRINCIPLE
4.1 SWITCHING ON
Push the On/Off switch on the back of the device.
See that the microprocessor control system activates.
Learn the functions of the control panel (Section 4.2.1.).
Set the values and start the operation according to section 4.3.
A supply fitted with a circuit breaker should be used for protection against indirect
contact in case of an isolation fault.

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4.2 DISPLAY AND CONTROL PANEL
1. Temperature display
6. “Operating the program” lamp
11. Back / Alarm mute button
2. Time display
7. Data transfer lamp
3. Encoder button
8. Alarm led
4. Start / Stop button
9. “End of the program” lamp
5. Heating lamp
10. Usb lamp
4.2.1 Explanations and Functions for Display and Control Panel
01-Temperature Display
This display shows
Sterilization chamber temperature during “stand-by” and during the operation (thermometer
position),
During program preparation, the temperature setting values or the alarm setting values,
Failure codes,
EoF warning when power is interrupted,
The set temperature values and alarm values.
02-Time Display
This display shows the values set for time during program preparation. Also; this display shows
he time values in the control of the settings.
03-Encoder Button
The encoder button has two physical movements. The button turn clockwise and anticlockwise to
increase or decrease the temperature and time values of the program. Also, the button press for
select / confirm.
04-Start / Stop Button
Used button to start the device to operating at set values or to stop the operation.