Olympus R1 User manual

This Quick Reference Guide provides instructions to use the Cell Counter model R1. For detailed instructions, please refer to the user manual in the supplied USB memory.
Using the R1 with Trypan Blue Stain
1. Select protocol
•Select protocol from the main menu.
•Select the DEFAULT or desired protocol.
•Make sure the dilution factor is set to 2 to count with
trypan blue.
•Press Load to apply the selected protocol.
2. Prepare & load sample
•Mix 10 µL cell suspension with 10 µL trypan blue.
Pipette gently.
•Open a new Cell Counting Slide.
•Hold the Cell Counting Slide by its edges and load
10 µL of the cell sample into a sample chamber.
3. Insert slide
•Insert the Cell Counting Slide face up and sample-
side first into the counting slide port of R1.
!
Important! Do not insert the Cell Counting Slide
facedown.
4. Count cells
•Select count from the main menu.
•Make sure the staining option is “With TB”.
•Press the circle next to [Autofocused Counting].
The circle will turn blue when the autofocus is
activated.
•Press [Count].
Results
1. Save and print results
•Insert a USB memory.
•Press [Save/Print] in the Results screen.
•Select the desired saving options (analyzed image, raw image, report).
•Name the count with the onscreen keyboard. Press Add Date/Time to add the date
and time to the name.
•Press Save. A folder will be created to contain the generated files.
•Press Print to get a printed report of the cell count results.
2. Image View
•Press [Image] to view the captured image of the analyzed cell sample.
•Press the magnifier button to zoom in and out of the image.
•Press Tag to label what was counted as live cells with green circles and dead cells
with red circles.
3. Histogram and Gating
•Press [Histogram & Gating] to see a graphical representation of the cell count
results.
•Press Total/on. The button will become Live/on and display the size distribution of
only live cells. Press Live/on. The button will become Dead/on and display the size
distribution of only dead cells.
•Select a light grey limit icon. Press the arrows to alter the size limits.
•Press Apply to set the size gating limits. The count results will adjust accordingly.
•Press Cell number to change the Y-axis to Cell Concentration.
•Press Cluster Map/off to change it to Cluster Map/on and show the distribution of
cell clusters.
4. Dilution Calculations
•Press [Dilution] to view the dilution calculator.
•Press [Total] to change the current concentration to [Live], the concentration of live
cells in the cell suspension.
•Input the values into the blanks for the desired final concentration and volume.
•Press Calculate to receive directions for dilution.
Cell Counter model R1
QUICK REFERENCE GUIDE
AX8818 Ver01

This Quick Reference Guide provides instructions to use the Cell Counter model R1. For detailed instructions, please refer to the user manual in the supplied USB memory.
Intended Use
The R1 is an electrical laboratory instrument for scientific research use only.
It is not a medical, therapeutic, or in vitro diagnostics device.
General Guidelines
Follow the instructions below to obtain the best results with the R1.
1. The R1 accurately detects and counts cells 3-60 µm in size at concentrations ranging from
5 x 104to 1 x 107cells/mL.
2. Perform cell counting within three minutes of mixing samples with trypan blue for accurate
cell viability measurements. If necessary, count your sample twice (duplicate readings) and
take an average.
3. Hold Cell Counting Slides by the edges to avoid touching the optical surface. Ensure that
the optical surfaces of the slide do not become smudged, damaged, or contaminated.
4. Do not reuse Cell Counting Slides. Used slides must be disposed as biohazardous waste
according to the rules and regulations of your local government.
5. As the R1 is calibrated before shipping, recalibration before use is not necessary. However,
if recalibration is needed, please refer to Section 2.3.3: Settings: Background Calibration in
the user manual.
Safety Precautions
Before using this instrument, read the user manual carefully to ensure that you know how to operate
it safely and correctly. To protect yourself and others from personal injury or damage to property, it is
essential that you read the warnings and information provided. Use the instrument as specified by
Olympus.
1. Install the instrument on a sturdy and level surface. Avoid vibrations from other devices.
2. Do not touch any of the components with wet hands.
3. Use the components provided or authorized by Olympus. If the proper combination of
components are not used, product safety performance cannot be guaranteed.
Ferrite Core Placement
Take a ferrite core and open it. Place the plug side of the
cable of the AC adapter in the ferrite core as shown in the
figure. Close the ferrite core.
The AC adapter cord and power cord are vulnerable to bending and twisting. Do not
apply excessive force.
Always use the AC adapter and power cord provided by Olympus. If the proper AC
adapter and power cord are not used, the electric safety and EMC (Electro-Magnetic
Compatibility) performance of the device cannot be guaranteed.
Connect the ground terminal of the power cord and that of the power outlet. If the
device is not grounded, our intended electric safety and EMC performance of the
device cannot be guaranteed.
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This device complies with the requirements of both directive 2004/108/EC concerning
electromagnetic compatibility and directive 2006/95/EC concerning low voltage. The CE
marking indicates compliance with the above directives. This system is applied with the
requirements of standard IEC/EN61326-1 concerning electromagnetic compatibility.
Immunity: Applied to industrial environment requirements.
Emission: Class A, applied to industrial environment requirements.
Some interference may occur if this system is used in a domestic location.
Cell Counter model R1
QUICK REFERENCE GUIDE