OPERATION
Your Omano microscope is simple to operate and with a little practice, you will soon become an expert microscopist. On monocular
microscopes, we recommend that you keep both eyes open in order to improve the viewing experience and to reduce eye strain.
Follow these steps to get started:
1. Rotate nosepiece to longest objective (40x or 100x and click in place. Place prepares slide on the stage. Carefully raise the stage by
turning the (coarse focus control until the stage just touches the objective. Then tighten the stage stop or locking nut on your microscope.
This will ensure that you cannot damage the slide or objective by inadvertently raising it too high.
2. Switch on the microscope and be sure to adjust the light intensity dial, if included on your microscope.
3. Rotate nosepiece so that 4x objective lens is in position for use. Place a prepared slide on the stage and clamp it carefully with the
moveable spring clip. Always begin with the lowest power objective.
4. Binocular Microscopes: Adjust the interpupillary distance of the oculars to suit you comfortably. Close your right eye and turn the
coarse focus control until a clear image is obtained. Use the fine focus control to optimize image clarity. Now close your left eye and
adjust the focus for your right eye by using the diopter on the right ocular.
5. When the desired view is obtained, rotate to next highest objective lens (10x . The nosepiece should click into position. Adjust the
fine focus if required. Repeat with 40x and 100x objectives as appropriate.
6. The iris diaphragm below the stage can be opened (slide lever forward or closed (slide lever back to left to control the amount of
light directed through the condenser. The condenser assembly under the stage can be shifted up or down by means of the control
knob (left hand side under stage , to effectively move the light beam closer to (up , or away from (down , the specimen under
observation. Try experimenting with various settings to get the most effective view of your specimen.
7. To center the condenser, first close the iris diaphragm. Adjust the condenser to its lowest setting and turn the condenser centering
screws until the light is centered when viewing through the eyepieces. Open the iris diaphragm until you see light on the sides of the
viewing area. If centered correctly, you will see a balance of light/shade around the viewing area. Adjust as necessary.
8. It is important to remember when adjusting the focus that the objective should never touch the specimen. Always use a cover slip on
your slides.
9. When using a 100x objective, the specimen will be very close to the slide. Apply a drop of cedar-wood oil or other immersion oil to the
cover slip. Clean the 100x oil immersion objective with a soft clean cloth or lens tissue to remove the immersion oil immediately after
using. It is strongly recommended to use a mechanical stage if using a 100x objective.
10. On most Omano compound microscopes, you can adjust the tension on the focus controls