Omax M8333S User manual

User Manual
Trinocular Infinity
Compound LED Microscope
Model M8333S series
MicroscopeNet.com

Table of Contents
i. Caution....................................................................................................1
ii. Care and Maintenance.............................................................................2
1. Components Illustration...........................................................................3
2. Installation ...............................................................................................5
3. Operation.................................................................................................7
4. Specifications ........................................................................................10
5. Optional Parts........................................................................................11
6. Troubleshooting Guide ..........................................................................13
7. Darkfield Condenser Installation and Operation Instructions..................15
8. Phase Contrast Kit Installation and Operation Instructions ....................17

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i. Caution
1. Open the carton carefully with a knife or paper cutter. Find the “UP” sign and place
the Styrofoam container on the side that makes the arrow upward. If the “UP”sign
is missing, please open the Styrofoam container gently to prevent any accessory
items (i.e. objectives or eyepieces) from dropping and being damaged.
2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any
other component, disconnect the power cord immediately and wipe up the spillage.
Otherwise, the instrument may be damaged.
5. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.
6. For safety when replacing the LED light and the fuse, be sure the main switch is
off, unplug the power cord, and replace the LED light and the fuse.
7. Important: confirm that the input voltage indicated on your microscope
corresponds to your line voltage (voltage switch is located on the back of the
microscope). The use of a different input voltage than indicated will cause severe
damage to the microscope.

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ii. Care and Maintenance
1. Do not attempt to disassemble any component including eyepieces, objectives or
focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on
metal surfaces should be cleaned with a damp cloth. More persistent dirt should be
removed using a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using
an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth
or cotton swab dampened with a lens cleaning solution (available at camera stores).
All optical lenses should be swabbed using a circular motion. A small amount of
absorbent cotton wound on the end of a tapered stick makes a useful tool for
cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as
this may cause problems with optical coatings or cemented optics or the flowing
solvent may pick up grease making cleaning more difficult. Oil immersion objectives
should be cleaned immediately after use by removing the oil with lens tissue or a
clean, soft cloth.
4. Observe the specimen with the 4X, 10X and 40X objectives in order, then observe
the specimen with the 100X objective. Apply the immersion oil on the slide cover with
the 100X objective. Do not let the immersion oil to contact with the dry objectives
lens (especially the 40X). Clean the dry objective lens using the lens cleaning paper
if the immersion oil is on the dry objectives lens. Clean the 100X objective lens first
using the lens cleaning paper after observing the specimen with the 100X objective,
then clean the specimen. More persistent dirt should be removed using a little bit
alcohol. Do not use organic solvents for cleansing.
5. Store the instrument in a cool, dry environment. Cover the microscope with the dust
cover when not in use.

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1. Components Illustration
11
12
13
14
15
16
17
7
18
7
1
2
3
4
5
6
7
8
9
10
19
7

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1
Eyepiece
11
Trinocular Viewing Head
21
Condenser Focus Knob
2
Diopter Ring
12
Swapping Lever
22
Top Lens Knob
3
Eyepiece Tube
13
Head Lock Thumb Screw
23
Stage Upward Stopper
4
Nosepiece
14
Objectives
24
Condenser
5
Slide Holder
15
Microscope Body
25
Aperture Diaphragm Ring
6
Mechanical Stage
16
Brightness Intensity Dial
26
Condenser Centering Screws
7
Light Collector
17
Focus Tension Ring
27
Filter
8
X-Y Stage Moving Knobs
18
Coarse Focus Knob
28
Color Filter Holder
9
Microscope Base
19
Fine Focus Knob
10
Photo Tube
20
Condenser Top Lens
20
21
22
24
25
3
26
27
23
28

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2. Installation
2.1 Installation of the trinocular viewing head
1) Loosen the head lock thumb screw on the top of the microscope body and remove
the plastic cover on the top.
2) Remove the cap on the dovetail of the trinocular viewing head.
3) Seat the dovetail of the viewing head (Fig. 1 (a)) into the socket on the top of the
microscope body (Fig. 1 (b)).
4) Rotate the viewing head as shown in Fig. 1 (c).
5) Tighten the head lock thumb screw (Fig. 1(d)).
Caution:
Do not release the viewing head until you are sure the viewing head is installed
securely.
2.2 Installation of the photo tube
1) Remove the cap on the top of the viewing head.
2) Thread the photo tube into the top of the viewing head.
2.3 Installation of the eyepieces
3) Remove the protective caps from the eyepiece tubes.
4) Insert the eyepieces into the eyepiece tubes.
2.4 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Turn the caps counter-clockwise to remove them from the nosepiece.
3) Take the objectives out from the plastic cases and turn each one clock-wise into the
holes on the nosepiece. Install the 4X objective into the nosepiece first. Then in a
Head Lock
Thumb Screw
Fig.1
(b)
(a)
(c)
(d)

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counter-clockwise direction, rotate the nosepiece and install each succeeding higher
magnification objective as shown in Fig. 2.
Note:
Inspect the objectives frequently for dirt or oil; clean if necessary.
Use the 10X objective to initially focus the image of your specimen.
When changing the objective magnification, rotate the objective nosepiece until you
hear a “click” sound or have a clear "in position" feeling. This ensures the objective
is centered in the optical light path.
2.5 Installation of the color filters
The color filter is simply put into the filter holder
2.6 Connecting the power cord
1) Turn the power switch to the off position.
2) Connect the power cord to the socket at the back of microscope body and plug the
other end into a wall outlet.
Caution:
Before connect the cord to the wall outlet; make sure the voltage switch is slide to
the correct position for the right power source.
2.7 Replacing the fuse
1) Turn off the power switch and disconnect the power
cord.
2) Find the fuse holder at the back of the microscope
body.
3) With a flat-head screwdriver, press and turn the
fuse holder counter clock wise to remove it.
4) Replace the old fuse with a new one,
5) Put the fuse holder back, press and turn it clock
wise. See Fig. 3.
Fig.2
Fig.3
Fuse

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2.8 Switching the power voltage
The power voltage can be switched between
115V and 230V.
1) Turn off the power switch and disconnect the
power cord.
2) Find the voltage switch on the back board. See
Fig 4
3) Push the switch down, the microscope will use
115V.
4) Push the switch up, the microscope will use 230V
3. Operation
3.1 Adjusting illumination
1) Plug the power cord into the power socket on the microscope and connect it to the
power outlet.
2) Turn on the power switch.
3) Rotate the brightness intensity dial to increase or decrease the brightness of the
illuminator.
3.2 Placing specimen
1) Place the slide on the mechanical stage.
2) Use the slide holder to gently secure the slide.
3) Turn the X and Y stage moving knobs to position the specimen in the center of
viewing field.
Caution:
Be sure not to allow an objective to touch a specimen slide when changing
objectives.
3.3 Focusing
1) With the 10X objective in position, raise the
mechanical stage using the coarse focus knob
until the specimen is close to the objective.
2) Turn the condenser top lens into light path.
3) Turn the coarse focus knob until the specimen
is in focus.
4) Use the fine focus knob to obtain a sharp
image.
5) Turn the condenser focus knob (Fig. 5) to
raise or lower the condenser till the image of
field is focused.
6) Turn the condenser centering screws (Fig. 5) to move the image of the field into the
center of the viewing field.
7) To get a good focused image, you may need to combine the focus knob adjustment
and interpupillary distance adjustment, along with eyepiece diopter adjustment
stated in 3.4 and 3.5.
8) You may now switch to another magnification objective.
Fig.4
Voltage Switch
Fig.5
Condenser Focus Knob
Top Lens Knob
Condenser Centering Screws

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Tips:
a) The condenser is raised when using high power
objectives and lowered when using low power
objectives.
b) The top lens is flipped up by turning the top lens
knob when using high power objectives.
c) To prevent your specimen slide from making
contact with an objective, raise the stage to its
highest position without contacting the 100X
objective; then tighten the stage upward stopper
(Fig. 6). Give the stage a tiny extra moving space
to ensure the objective can be focused every time.
3.4 Adjusting interpupillary distance
While observing with both eyes, hold the left and right eyepiece tubes then swing
them around the center axis. The interpupillary distance is correct when the left and
right fields of view converge completely into one image.
3.5 Adjusting eyepiece diopter
1) Using the 10X objective and your right eye only, observe your specimen through the
eyepiece and bring it into focus by adjusting the focus knobs.
2) Then observe the specimen with your left eye only through the left eyepiece. If the
specimen is not in focus, turn the diopter ring on the eyepiece tube until a sharp
image is obtained.
3.6 Applying the immersion oil
1) Rotate the objective nosepiece to seat the observing position between the 40X and
100X objectives as shown in Fig. 7 (a).
2) Place a drop of immersion oil on the slide cover as shown in Fig. 7 (b).
3) Rotate the objective nosepiece to seat the 100X objective to the observing position
until you hear a “click” sound.
4) After observing the specimen, use the lens cleaning paper to clean the 100X
objective lens gently and the specimen in time.
5) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens and
the specimen.
Fig.6
Stage Upward Stopper
Fig.7
(b)
(a)

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Caution (important):
When you use the 100X objective to observe the specimen, you have to finish
observing the specimen with the 4X, 10X, 40X objectives.
When you use the 100X objective to observe the specimen, you have to apply the
immersion oil on the top of the slide cover.
When you apply the immersion oil with the 100X objective, do not let the immersion
oil to contact with the dry objective lenses (especially the 40X). If the immersion oil
is on the dry objectives lens, please use the lens cleaning paper to clean the
objectives lens in time. The oil will damage the dry objective lenses.
After observing the specimen with the 100X objective, clean the 100X objective lens
first.
3.7 Adjusting iris aperture diaphragm
Turn the aperture diaphragm ring to adjust the aperture size.
3.8 Adjusting focus knob tension
The tightness of the focus knob tension has been pre-set at the factory. If the
mechanical stage drops by itself, rotate the focus tension ring (Fig. 8) with the
tension wrench situated between the coarse focus knob and microscope body until
the tension is in maintained.
3.9 Photo/video observing, capturing and recording
1) Insert camera into the photo tube (Fig. 9).
2) Bring the microscope into focus by following the
procedures in 3.3.
3) Pull the swapping lever out (Fig. 10)
4) Adjust the photo tube length until image on camera is in
focus.
Note:
The swapping lever to switch beam spilt for photo part and
100% to eyepieces when photo tube is not in use.
(a)
Focus Tension Ring
Fig.8
Fig.9
Camera
Fig.10

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4. Specifications
Model
M8333S
Total Magnification
40X, 80X, 100X, 200X, 400X, 800X, 1000X, 2000X
Eyepieces
1 pair of WF10X/18
1 pair of WF20X
Objectives
PLAN Infinity objectives achromatic
4X/0.10 ∞/-
10X/0.25 ∞/0.17
40X/0.65 ∞/0.17 (spring)
100X/1.25 ∞/0.17 (spring, oil)
Viewing Head
30ºinclined, 360ºswiveling siedentopf trinocular viewing head
Hinge interpupillary distance adjustment, 2-3/16” ~ 2-5/16”
(55mm ~ 75mm)
Diopter adjustment on left eyepiece tube
A lever to switch between beam spilt for photo part and 100% to
eyepieces when camera is not in use
Nosepiece
Reversed revolving quadruple nosepiece
Stage
Double layer mechanical stage
Dimension: 5-1/2” x 4-1/4” (140mm x 110mm)
Translation range: 3” x 1-13/16” (78mm x 46mm)
Condenser
NA=0.9/0.25, with a flip up top lens, built-in aperture iris
diaphragm
Center adjustable, rack and pinion adjustment
Focus Mechanism
Coaxial coarse and fine focusing knobs on both sides with focus
stop and tension control
Collector
With color filter holder
Color filter
45mm green filter in diameter
Illumination
Transmitted, 3W LED, intensity adjustable
Power Supply
AC 115V / 230V switchable (US and Canada plug)
Cameras (optional)
Refer to the cameras specifications
Darkfield Condensers (optional)
Refer to the darkfield condensers specifications
Phase Contrast Kit (optional)
Refer to the phase contrast kit specifications
Dimension
19-3/4” x 16” x 8” (50 cm x 41 cm x 20 cm)
Net weight
17 lbs (7.7 kg)

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5. Optional Parts
(The optional parts may be included in some models or sold separately.)
1) Cameras
Model
Sensor
Resolution
Operating System
Software
A35100U
CMOS
USB2.0
3584 x 2748 (10.MP)
MS Windows
Mac OS 10.8 and up
Linux (2.6 or above)
Included
A35140U
4096 x 3288 (14.0MP)
A3550U3
CMOS
USB3.0
2560 x 1922 (5.0MP)
A3580U3
3328 x 2548 (8.0MP)
A35100U3
3584 x 2746 (10.0MP)
A35140U3
4096 x 3286 (14.0MP)
A35180U3
4912 x 3684 (18.0MP)
CPZJ-150R
CMOS
2560 x 1920 (5.0MP)
N/A
N/A

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2) Darkfield Condensers
Model
Darkfield
Condenser
Numerical
Aperture
Objective
Mounting
Size(diameter)
A191-INF
Dry
0.7-0.9
-
37mm
A191BD-
INF
Oil
1.36-1.25
-
37mm
3) Phase Contrast Kits
Model
Phase Contrast Objective
Condenser
Annular Ring Plates
Centering
Telescope
A1PHIP
Plan achromatic 10X with
built-in phase plate
Plan achromatic 20X with
built-in phase plate
Plan achromatic 40X with
built-in phase plate, spring
Plan achromatic 100X with
built-in phase plate, spring,
oil
NA 1.25
Five positions:
10 for 10X phase contrast
objective
20 for 20X phase contrast
objective
40 for 40X phase contrast
objective
100 for 100X phase
contrast objective
B for bright field
observation, with iris
diaphragm
Focusing
adjustable

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6. Troubleshooting Guide
Problem
Cause
Solution
Lamp does not
light when
switched on
No electrical power
Check power cord connection
LED or power unit dead
Replace LED light
Fuse blown out
Replace fuse
Darkness at the
periphery or
uneven brightness
in the field of view
Revolving nosepiece not in click stop
position
Revolve the nosepiece to click-stop
position by swinging the objective
correctly into the optical path
The field iris diaphragm are not
opened enough
Open field diaphragm
The field diaphragm not in the center
Center the field iris diaphragm
Dirt or dust on the
view
Dirt or dust on the eyepiece,
condenser, objective, collector lens
or specimen
Clean the lens with a lens cleaning paper
Poor image
quality or not able
to get focused
image
No slide cover attached to the slide
Attach a 0.17mm slide cover
Slide cover is too thick or thin
Use a slide cover of the appropriate
thickness (0.17mm)
Slide may be upside down
(specimen at the bottom)
Turn slide over so the cover-glass faces
up
Diopter adjustment is not set
properly
Readjust the diopter settings
Immersion oil is on a dry objective
(especially the 40X)
Check the objectives, clean if necessary
No immersion oil used with 100X
objective
Use immersion oil
Air bubbles in immersion oil
Remove bubbles
Aperture is closed or open too much
Open or close properly
Condenser top lens not in use
Flip up the top lens when using high
power objective
Condenser is not in the right position
Adjust the condenser
The field diaphragm not in the
center
Center the field iris diaphragm

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The aperture and field iris diaphragm
are not opened enough
Open and adjust the aperture and field
diaphragm
Specimen rises from stage surface
Secure the specimen in the slide holder
Revolving nosepiece is not in the
click-stop position
Revolve the nosepiece to the click-stop
position
Lamp intensity is too high or low
Adjust the light intensity by rotating the
intensity control dial
Slippage of focus
when using the
coarse focusing
knob fine focus is
ineffective
Tension adjustment is set too low
Increase the tension on the focusing
knobs
Tension adjustment is set too high
Loosen the tension on the focusing knobs

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7. Darkfield Condenser Installation and Operation
Instructions
7.1 Dry darkfield condenser of A191-INF
1) Mounting the dry darkfield condenser
Loosen the condenser lock thumb screw on the condenser holder and remove the
brightfield condenser as shown in Fig. 11 (a).
Install the dry darkfield condenser and tighten the condenser lock thumb screw on
the condenser holder as shown in Fig. 11 (c).
2) Centering the dry darkfield condenser
Turn the 4X objective to the light path.
Turn the condenser focus knob to lower the
condenser till a dark spot showed in the viewing field
as shown in Fig. 12 (a).
Turn the condenser translational centering screws to
move the dark spot to the center as shown in Fig. 12 (b).
3) Place the slide on the stage.
4) Raise the condenser all the way to the top and lower it a little bit.
5) Following the procedures in this manual to focus and observe.
6) Move the condenser up or down slightly to get the best darkfield viewing.
Note：
The dry darkfield condenser is used with the dry objectives only.
The dry darkfield condenser works with the 4X, 10X, 40X objectives.
The dry darkfield condenser won’t work with the 100X oil immersion objective.
(a) (b)
Fig.11
(a)
(b)
(c)
Fig.12
(a)
(b)
Condenser Centering Screws
(C)
Condenser Lock Thumb Screw
(c)
Condenser Holder

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7.2 Oil darkfield condenser of A191BD-INF
1) Mounting the oil darkfield condenser
Loosen the condenser lock thumb screw on the condenser holder and remove the
brightfield condenser as shown in Fig. 13 (a).
Install the oil darkfield condenser and tighten the condenser lock thumb screw on
the condenser holder as shown in Fig. 13 (c).
2) Centering the oil darkfield condenser
Turn the 40X objective to the light path.
Turn the condenser focus knob to slowly lower and
raise the condenser till a dark spot showed in the
viewing field as shown in Fig. 14 (a).
Turn the condenser translational centering screws to
move the dark spot to the center as shown in Fig. 14 (b).
3) When using the 40X objective to observe the specimen.
Place the slide on the stage.
Lower the condenser until a sharp brightfield image showing in the viewing field.
Raise the condenser until the darkfield image showing in the viewing field.
4) When using the 100X objective to observe the specimen
Raise the condenser until the top is close to the opening of stage.
Place a drop of immersion oil on the top of condenser.
Place the slide on the stage.
5) Raise the condenser and let the oil drop contact the bottom of the slide. If air
bubbles exist in the oil, clean the oil from the top of the condenser and bottom of
slide with a lens cleaning paper and repeat the procedures.
6) Follow the procedures in this manual to bring the sample on slide in focus and
observe.
7) After observing the specimen with 100X objective, use the lens cleaning paper to
gently clean the 100X objective lens, oil darkfield condenser and the specimen in
time.
8) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens, oil
darkfield condenser and the specimen.
Fig.14
(a)
(b)
Fig.13
(a)
(b)
(c)
Condenser Centering Screws
(C)
Condenser Lock Thumb Screw
(c)
Condenser Holder

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Note：
When using the 40X objective, you won’t need to apply oil drop on the oil condenser.
When using the 100X objective, the condenser won’t work well if no oil drop applied
on the condenser.
When you apply the immersion oil with the 100X objective, do not let the immersion
oil to contact with the dry objective lenses (especially the 40X). If the immersion oil
is on the dry objectives lens, please use the lens cleaning paper to clean the
objectives lens in time. The oil will damage the dry objective lenses.
After observing the specimen with the 100X objective, clean the 100X objective lens
first.
8. Phase Contrast Kit Installation and Operation
Instructions
8.1 Phase contrast kit of A1PHIP:
1) Mounting the phase contrast objectives
a. Take off all the objectives from the nosepiece.
b. Install the phase contrast objectives onto the nosepiece following the steps in 2.4.
2) Mounting the phase contrast condenser/annular ring disk
a. Rotate the nosepiece and set the 10X objective in position.
b. Turn the coarse focus knob to raise the mechanical stage to the highest position
without contacting the 10X objective.
c. Turn the condenser control knob to lower the condenser to the lowest position.
d. Loosen the condenser lock thumb screw and remove the brightfield condenser as
shown in Fig. 15 (a).
e. Insert the condenser/annular ring disk into the condenser holder, and tighten the
thumb screw as shown in Fig. 15 (c).
f. Re-adjust the condenser height to its normal position.
g. Re-adjust the mechanical stage height to its normal position.
Condenser Holder
Fig.15
Condenser Lock Thumb Screw
Condenser Centering Screws
(a)
(b)
(c)

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(a) (b)
(c) (d)
Note:
When raising the mechanical stage, do not make
contact with the objective.
3) Turn the desired objective into light path.
4) Turn the annular ring disk to put the corresponding
ring into light path, i.e. if you are using the 40X
phase contrast objective, you should turn the disk
at 40 as shown in Fig. 16.
5) Centering the annular ring.
a. Remove one eyepiece from the microscope eyepiece tube and
insert the centering telescope as shown in Fig. 17.
b. Observe from the telescope. The bright ring and dark ring
should be coincided with each other as shown in Fig.18 (d).
c. If the ring images are not clear, turn the top of telescope
until both ring images are in focus.
d. If the bright ring is still obscure as in Fig.18 (b), adjust
the condenser focus knob.
e. If the two ring images are not coincided as shown in
Fig.18 (c):
Turn the condenser centering screws (Fig. 19) to
move the dark spot to the center until two ring
images are coincided, or
Turn the adjustment knobs (Fig. 19) to move the
dark spot to the center until two ring images are
coincided.
f. Remove the centering telescope and replace it with
the eyepiece.
g. Put the specimen on the stage and adjust the
illumination, focusing, etc following the instructions in
this manual.
Note:
The phase contrast condenser will be working as a
conventional Abbe condenser if the annular ring
disk being put at BF position.
The phase contrast condenser will be working as a
dry darkfield condenser if the annular ring disk
being put at DF position.
6) Performing the phase contrast observation.
After centering the condenser ring plate, perform
the phase contrast observation the same way as a normal
bright field microscope.
Note:
When change to another phase contrast objective and corresponding condenser
ring plate, the focusing and centering of bright ring and dark ring should be repeated
following the procedures from 5)-b to 5)-f.
Fig.17
Fig.16
40
Fig.18
Fig.19
Adjustment Knobs
Condenser Centering Screws