Omax M8311Z Series User manual

User Manual
Trinocular Compound
LED Microscope
Model M8311Z Series
MicroscopeNet.com

Table of Contents
i. Caution ..................................................................................................1
ii. Care and Maintenance..........................................................................2
1. Components Illustration ........................................................................3
2. Installation.............................................................................................4
3. Operation..............................................................................................6
4. Specifications........................................................................................9
5. Optional Parts.....................................................................................10
6. Troubleshooting Guide........................................................................13
7. Darkfield Condenser Installation and Operation Instructions...............15
8. Phase Contrast Kit Installation and Operation Instructions..................16

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i. Caution
1. Open the carton carefully with a knife or paper cutter. Find the “UP” sign and place
the Styrofoam container on the side that makes the arrow upward. If the “UP”sign
is missing, please open the Styrofoam container gently to prevent any accessory
items (i.e. objectives or eyepieces) from dropping and being damaged.
2. Do not discard the molded Styrofoam container. The container should be retained
should the microscope ever requires reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty
environments. Ensure that the microscope is located on a smooth, level and firm
surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any
other component, disconnect the power cord immediately and wipe up the spillage.
Otherwise, the instrument may be damaged.
5. All electrical connectors (power cord) should be inserted into an electrical surge
suppressor to prevent damage due to voltage fluctuations.

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ii. Care and Maintenance
1. Do not attempt to disassemble any component including eyepieces, objectives or
focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on
metal surfaces should be cleaned with a damp cloth. More persistent dirt should be
removed using a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using
an air stream from an air bulb. If dirt remains on the optical surface, use a soft cloth
or cotton swab dampened with a lens cleaning solution (available at camera stores).
All optical lenses should be swabbed using a circular motion. A small amount of
absorbent cotton wound on the end of a tapered stick makes a useful tool for
cleaning recessed optical surfaces. Avoid using an excessive amount of solvents as
this may cause problems with optical coatings or cemented optics or the flowing
solvent may pick up grease making cleaning more difficult. Oil immersion objectives
should be cleaned immediately after use by removing the oil with lens tissue or a
clean, soft cloth.
4. Observe the specimen with the 4X, 10X and 40X objectives in order, then observe
the specimen with the 100X objective. Apply the immersion oil on the slide cover with
the 100X objective. Do not let the immersion oil to contact with the dry objectives
lens (especially the 40X). Clean the dry objective lens using the lens cleaning paper
if the immersion oil is on the dry objectives lens. Clean the 100X objective lens first
using the lens cleaning paper after observing the specimen with the 100X objective,
then clean the specimen. More persistent dirt should be removed using a little bit
alcohol. Do not use organic solvents for cleansing.
5. Store the instrument in a cool, dry environment. Cover the microscope with the dust
cover when not in use.

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1. Components Illustration
1
Photo Tube
9
Microscope Base
17
Power Switch
2
Eyepiece
10
Head Lock Thumb Screw
18
Brightness Intensity Dial
3
Diopter Ring
11
Nosepiece
19
Condenser Lock Thumb Screw
4
Eyepiece Tube
12
Objective
20
Condenser Focus Knob
5
Viewing Head
13
Microscope Body
21
Condenser
6
Slide Holder
14
Coarse Focus Knob
22
Color Filter Holder
7
Mechanical Stage
15
Fine Focus Knob
8
Light Collector
16
X-Y Stage Moving Knobs
2
3
4
5
6
7
8
9
1
11
12
13
14
15
16
17
18
10
20
21
22
19

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2. Installation
2.1 Installation of the trinocular viewing head
1) Loosen the head lock thumb screw on the top of the microscope body and remove
the plastic cover on the top.
2) Remove the cap on the dovetail of the trinocular viewing head.
3) Seat the dovetail of the viewing head into the socket on the top of the microscope
body and tighten the head lock thumb screw.
Caution:
Do not release the viewing head from your hand grip until you are sure the viewing
head is installed securely.
2.2 Installation of the photo tube
1) Remove the cap on the top of the viewing head.
2) Thread the photo tube into the top of the viewing head.
2.3 Installation of the eyepieces
1) Remove the protective caps from the eyepiece tubes.
2) Insert the eyepieces into the eyepiece tubes.
2.4 Installation of the objectives
1) Adjust the coarse focus knob until the mechanical stage is at its lowest position.
2) Turn the caps counter-clockwise to remove them from the nosepiece.
3) Take the objectives out from the plastic cases and turn each one clock-wise into the
holes on the nosepiece. Install the 4X objective into the nosepiece first. Then in a
counter-clockwise direction, rotate the nosepiece and install each succeeding higher
magnification objective as shown in Fig. 1.
Note:
Inspect the objectives frequently for dirt or oil; clean if necessary.
Use the 10X objective to initially focus the image of your specimen.
When changing the objective magnification, rotate the objective nosepiece until you
hear a “click” sound or have a clear "in position" feeling. This ensures the objective is
centered in the optical light path.
Fig.1

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2.5 Installation of the glass filter
1) Swing out the color filter holder under the condenser.
2) Place the filter into the color filter holder as shown in Fig. 2, swing the holder in.
2.6 Replacing the fuse
1) Turn off the power switch and disconnect the power cord.
2) Find the fuse holder at the rear bottom of the microscope body.
3) Pry out the fuse holder gently with a flat head screwdriver.
4) Replace the old fuse with a new one, and then insert it back in. See Fig. 3.
2.7 Installation of the camera (optional, may not included
in your package)
1) Take off the plastic cover on the photo tube.
2) Insert the camera into the photo tube, and then connect
the camera to a computer via USB2.0 cable. See Fig.4.
3) The manual for the camera is on a CD (or mini CD). Refer
to the manual to install the driver and software on to the
computer.
4) The camera is optional and may have different color and
shape from the one in the figure, depending on the
model purchased.
2.8 Installation of darkfield condensers (optional, may not
included in your package)
Please see section 7 (Darkfield Condenser Installation and Operation Instructions)
2.9 Installation of phase contrast kits (optional, may not included in your package)
Please see section 8 (Phase Contrast Kit Installation and Operation Instructions)
Camera
Photo Tube
Fig.3
Fig.4
Color Filter
Color Filter Holder
Fig.2
Fuse Holder
Fuses
Fig.3

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3. Operation
3.1 Adjusting illumination
1) Plug the power cord into the power socket on the microscope and connect it to the
power outlet.
2) Turn on the power switch.
3) Rotate the brightness intensity dial to increase or decrease the brightness of the
illuminator.
Caution:
A diffusion filter is attached beneath the condenser to get uniform light and protect
your eyes from strong LED light when a low power objective applies. The diffusion
filter can be swung out to make the view field brighter when observing with a high
power objective, such as 100X objective.
3.2 Placing specimen
1) Place the slide on the mechanical stage.
2) Use the slide holder to gently secure the slide.
3) Turn the X and Y stage moving knobs to position the specimen in the center of
viewing field.
Caution:
Be sure not to allow an objective to touch a specimen slide when changing objectives.
3.3 Focusing
1) With the 10X objective in position, raise the mechanical
stage using the coarse focus knob until the specimen is
close to the objective.
2) Turn the coarse focus knob until the specimen is in
focus. Then use the fine focus knob to obtain a sharp
image. You may now switch to another magnification
objective.
3) To get a good focused image, you may need to combine
the focus knob adjustment and interpupillary distance
adjustment, along with eyepiece diopter adjustment
stated in 3.4 and 3.5.
Tips:
To prevent your specimen slide from making contact with an objective, turn the 100X
objective in position and adjust the thumb screw of focus stop (as shown in Fig.5) so
that the 100X objective will not contact the specimen while the stage is adjusted to its
highest position. Give the stage a tiny extra moving space to ensure the objective can
be focused every time.
3.4 Adjusting interpupillary distance
While observing with both eyes, hold the left and right eyepiece tubes then swing
them around the center axis. The interpupillary distance is correct when the left and
right fields of view converge completely into one image.
3.5 Adjusting eyepiece diopter
1) Using the 10x objective and your right eye only, observe your specimen through the
eyepiece and bring it into focus by adjusting the focus knobs.
Fig.5
Focus Stop

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2) Then observe the specimen with your left eye only through the left eyepiece. If the
specimen is not in focus, turn the diopter ring on the eyepiece tube until a sharp
image is obtained.
3.6 Applying the immersion oil
1) Rotate the objective nosepiece to seat the observing position between the 40X and
100X objectives as shown in Fig. 6 (a).
2) Place a drop of immersion oil on the slide cover as shown in Fig. 6 (b).
3) Rotate the objective nosepiece to seat the 100X objective to the observing position
until you hear a “click” sound.
4) After observing the specimen, use the lens cleaning paper to clean the 100X objective
lens gently and the specimen in time.
5) If it is hard to clean, you need a little bit alcohol to clean the 100X objective lens and
the specimen.
Caution (important):
When you use the 100X objective to observe the specimen, you have to finish
observing the specimen with the 4X, 10X, 40X objectives.
When you use the 100X objective to observe the specimen, you have to apply the
immersion oil on the top of the slide cover.
When you apply the immersion oil with the 100X objective, do not let the
immersion oil to contact with the dry objective lenses (especially the 40x). If the
immersion oil is on the dry objectives lens, please use the lens cleaning paper to
clean the objectives lens in time. The oil will damage the dry objective lenses.
After observing the specimen with the 100X objective, clean the 100X objective
lens first.
3.7 Adjusting condenser
1) Turn the condenser focus knob to raise or lower the condenser.
2) The condenser is raised when using high magnification objectives and lowered when
using low magnification objectives.
Note:
The centering of the condenser and the light axis
of the objective are factory adjusted. Do not
attempt to re-adjust.
The highest position of the condenser has been
factory adjusted. Do not attempt to re-adjust.
3.8 Adjusting iris aperture diaphragm
Swing the iris diaphragm lever (Fig.7) left or right to
adjust the aperture size.
(a)
Fig.6
(b)
(a)
Iris Diaphragm Lever
Fig.7

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Note:
The iris diaphragm is designed to adjust the aperture size, not to adjust the brightness
although the brightness will be changed when it's adjusted. When aperture is
adjusted to smaller size, the contrast will be increased and the depth of field will be
increased as well. Turn up the intensity of the light if the image is too dim.
3.9 Adjusting focus knob tension
The tightness of the focus knob tension has been
pre-set at the factory. If the mechanical stage drops
by itself, rotate the tension adjustment ring (Fig.8)
located inside the focus knob on the power switch
side until the tension is in maintained.
3.10 Cameras (optional, may not included in your
package)
1) Bring the microscope into focus by following the procedures in
3.3.
2) Install the camera by following the procedures in 2.7.
3) Open image observing software to examine (more details
see camera's manual).
4) If the image is not clear, loosen the 2 set screws (See Fig.9)
on the photo tube, turn the upper half part to lower down or
raise up the camera mounted on the top, till the image is
clear.
5) You can also capture images or record live videos through
the software, depending on the functions provided by the
software.
Note:
Please refer to the manual in the camera’s CD for the details of installation and
operation of the camera.
3.11 Darkfield condensers observation (optional, may not included in your package)
Please see section 7 (Darkfield Condenser Installation and Operation Instructions)
3.12 Phase contrast kits observation (optional, may not included in your package)
Please see section 8 (Phase Contrast Kit Installation and Operation Instructions)
Set Screw
Fig.8
Fig.9
Tension adjustment Ring

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4. Specifications
General
Model
M8311Z series
Total Magnification
40X, 100X, 250X, 400X, 1000X, 2500X
Viewing Head
30ºinclined, 360ºswiveling Siedentopf trinocular viewing head
Interpupillary distance 1-7/8” ~ 2-15/16” (48mm ~ 75mm)
Adjustable diopter on left eyepiece tubes
Eyepieces
1 pair of WF10X/18
1 pair of WF25X
Objective Tube Length
160mm
Nosepiece
Reversed revolving quadruple
Objectives
Achromatic 4X, 10X, 40X(spring), 100X(spring, oil)
Condenser
Abbe, NA=1.25, with iris aperture diaphragm and filter holder
Rack and pinion focus adjustment
Focus Mechanism
Coaxial coarse and fine focusing knobs on both sides with
stop
Stage
Double layer mechanical stage
Dimension: 5-1/2”x 4-1/4” (140mm x 110mm)
Translation range: 3” x 1-9/16” (78mm x 40mm)
Illumination
Transmitted: replaceable 3W LED, variable intensity
Darkfield Condensers (optional)
Refer to the darkfield condensers specifications
Phase Contrast Kits (optional)
Refer to the phase contrast kits specifications
Cameras (optional)
Refer to the cameras specifications
Carrying Case (optional)
Refer to the carrying case specifications
Power Supply
AC 110V~240V, 50/60Hz (US and Canada plug)
Dimension
12-3/16” x 8” x 17-3/8” (31cm x 20cm x 44cm)
Net weight
11 lb 6.5 oz (5.2 kg)

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5. Optional Parts
(The optional parts may be included in some models or sold separately.)
1) Cameras
Model
Sensor
Resolution
Operating System
Software
A1502
CMOS
USB2.0
640 x 480 (0.3MP)
MS Windows
Mac OS X 10.6-10.8
Included
A3513U
1280 x 1024 (1.3MP)
MS Windows
Mac OS 10.8 and up
Linux (2.6 or above)
A3520U
1600 x 1200 (2.0MP)
A3530U
2048 x 1536 (3.0MP)
A3550U
2592 x 1944 (5.0MP)
A3590U
3488x2616 (9.0MP)
A35100U
3584 x 2748 (10.MP)
A35140U
4096 x 3288 (14.0MP)
A3550U3
CMOS
USB3.0
2560 x 1922 (5.0MP)
A35100U3
3584 x 2746 (10.0MP)
A35140U3
4096 x 3286 (14.0MP)
A35180U3
4912 x 3684 (18.0MP)
CPZJ-150R
CMOS
2560 x 1920 (5.0MP)
N/A
N/A

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2) Darkfield Condensers
Model
Darkfield
Condenser
Numerical
Aperture
Objective
Mounting
Size(diameter)
A191
Dry
0.7-0.9
-
37mm
A191BD
Oil
1.36-1.25
-
37mm
A191BOIL
(new design)
Oil
1.36-1.25
Plan 100X/1.25 oil-0.5
160/0.17(spring), with
iris diaphragm
37mm
3) Carrying Case
Model
Dimension
Net Weight
A187V
9-7/8" x 11" x 18-1/2"
(25cm x 28cm x 47cm)
2 lb 6 oz (1.1 kg)
A185A
18-1/8" x 13" x 13-3/8"
46cm x 33cm x 34cm
10 lb 14 oz (4.93 kg)

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4) Phase Contrast Kits
Model
Objectives
Condenser
Annular Ring Plates
Centering
Telescope
A1PHB1
Plan achromatic 10X with
built-in phase plate
Plan achromatic 20X with
built-in phase plate
Plan achromatic 40X with
built-in phase plate, spring
Plan achromatic 100X with
built-in phase plate, spring,
oil
NA 1.25
five positions:
10 for 10X phase contrast
objective
20 for 20X phase contrast
objective
40 for 40X phase contrast
objective
100 for 100X phase contrast
objective
B for bright field observation,
with iris diaphragm
focusing
adjustable
A1PHB3
Achromatic 10X with built-in
phase plate
Achromatic 20X with built-in
phase plate
Achromatic 40X with built-in
phase plate, spring
Achromatic 100X with built-in
phase plate, spring, oil
five positions:
10 for 10X phase contrast
objective
20 for 20X phase contrast
objective
40 for 40X phase contrast
objective
100 for 100X phase contrast
objective
B for bright field observation,
with iris diaphragm
A1PHC2
Achromatic plan 10X with
built-in phase plate
Achromatic plan 40X with
built-in phase plate, spring
Four positions:
10/20 for 10X/20X phase
contrast objective
40 for 40X phase contrast
objective
DF for darkfield observation
BF for brightfield
observation, with iris
diaphragm
A1PHD
Achromatic 10X with built-in
phase plate
Achromatic 40X with built-in
phase plate, spring
Achromatic 100X with built-in
phase plate, spring, oil
10 for 10X phase contrast
objective
40 for 40X phase contrast
objective
100 for 100x phase contrast
objective

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6. Troubleshooting Guide
GENERAL PROBLEMS
Problem
Cause
Solution
Lamp does not light
when switched on
No electrical power
Check power cord connection
LED or power unit dead
Contact seller for service
Fuse blown out
Replace fuse
Darkness at the
periphery or uneven
brightness in the field
of view
Revolving nosepiece not in click stop
position
Revolve the nosepiece to click-stop
position by swinging the objective
correctly into the optical path
Dirt or dust on the
view
Dirt or dust on the eyepiece,
condenser, objective, collector lens or
specimen
Clean the lens with a lens cleaning
paper
Poor image quality or
not able to get
focused image
No slide cover attached to the slide
Attach a 0.17mm slide cover
Slide cover is too thick or thin
Use a slide cover of the appropriate
thickness (0.17mm)
Slide may be upside down (specimen
at the bottom)
Turn slide over so the cover-glass faces
up
Diopter adjustment is not set properly
Readjust the diopter settings
Immersion oil is on a dry objective
(especially the 40X)
Check the objectives, clean if necessary
No immersion oil used with 100X
objective
Use immersion oil
Air bubbles in immersion oil
Remove bubbles
Condenser aperture is closed or open
too much
Open or close properly
Condenser is positioned too low
Position the condenser upward
Specimen rises from stage surface
Secure the specimen in the slide holder
Revolving nosepiece is not in the
click-stop position
Revolve the nosepiece to the click-stop
position
Lamp intensity is too high or low
Adjust the light intensity by rotating the
intensity control dial

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Slippage of focus
when using the coarse
focusing knob Fine
focus is ineffective
Tension adjustment is set too low
Increase the tension on the focusing
knobs
Tension adjustment is set too high
Loosen the tension on the focusing
knobs
DARKFIELD PROBLEMS
Problem
Cause
Solution
Totally dark in the
viewing field
The light is not on
Turn on the light
(A191BOIL only) There is no oil
in between the condenser top
lens and slide
Place a drop of oil on the top lens of
condenser and let it contact the underside of
slide
(A191BOIL only) The built-in iris
diaphragm of 100X objective is
not in proper position
Adjust the iris diaphragm ring on the objective
The condenser is not in the right
position
Lower or raise the condenser slightly to the
position that the specimen is the brightest.
(A191BOIL only) Make sure the oil contact the
bottom of the slide and the top of the
condenser during the adjustment.
The illumination is
insufficient or too
bright
The intensity of lamp is too low
Adjust the intensity dial to increase the
brightness
The condenser is not in the right
position
Lower or raise the condenser slightly to the
position that the specimen is the brightest.
(A191BOIL only) Make sure the oil contact the
bottom of the slide and the top of the
condenser during the adjustment.
The condenser is not centered
properly
Adjust the translational centering screws to
center the condenser
(A191BOIL only) The built-in iris
diaphragm of 100X objective is
not in proper position
Turn the iris diaphragm ring on the objective
Image of the
specimen is not clear
and lacking in
sufficient contrast
The specimen is not suitable for
darkfield observation
Change to brightfield or phase contrast
(A191BOIL only) The built-in iris
diaphragm of 100X objective is
not in proper position
Turn the iris diaphragm ring on the objective
The condenser is not in the right
position
Lower or raise the condenser slightly to the
position that the specimen is the brightest.
(A191BOIL only) Make sure the oil contact the
bottom of the slide and the top of the
condenser during the adjustment.

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7. Darkfield Condenser Installation and
Operation Instructions
7.1 Dry darkfield condenser of A191
1) Mounting the dry darkfield condenser
Loosen the condenser lock thumb screw on the
condenser holder and remove the brightfield
condenser.
Install the dry darkfield condenser and tighten
the condenser lock thumb screw on the
condenser holder (see Fig. 10).
2) Centering the dry darkfield condenser
Turn the 4X objective to the light path.
Turn the condenser focus knob to lower the
condenser till a dark spot showed in the viewing
field as shown in Fig. 11 (a).
Turn the condenser translational centering screws
to move the dark spot to the center as shown in
Fig. 11 (b).
3) Place the slide on the stage.
4) Raise the condenser all the way to the top and then
lower it a little bit.
5) Follow the procedures in this manual to bring the sample on slide in focus and
observe.
6) Move the condenser up or down slightly to get the best darkfield viewing.
Note：
The dry darkfield condenser is used with the dry objectives only.
The dry darkfield condenser works with the 4X, 10X, 40X objectives.
The dry darkfield condenser does not work with the 100X oil immersion objective.
7.2 Oil darkfield condenser of A191BOIL
1) Mounting the oil darkfield condensers
Loosen the condenser lock thumb screw on the
condenser holder and remove the brightfield
condenser.
Install the oil darkfield condensers and tighten
the condenser lock thumb screw on the
condenser holder (see Fig. 12).
2) Centering the oil darkfield condensers
Turn the 40X objective to the light path.
Turn the condenser focus knob and slowly lower
or raise the condenser till a dark spot showed in
the viewing field as shown in Fig. 13 (a).
Turn the condenser translational centering
screws to move the dark spot to the center as
shown in Fig. 13 (b).
(a) (b)
(a) (b)
Fig.10
Fig.12
Fig.13
Fig.11
Condenser Lock
Thumb Screw
Centering Screws
Condenser Holder
Condenser Lock
Thumb Screw
Centering Screws
Condenser Holder

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3) Raise the condenser till the top lens is close to the opening of stage. Place a drop of
immersion oil on the top of the lens of the condenser.
4) Place the slide on the stage. Raise the condenser and let the oil
drop contact the bottom of the slide. If air bubbles exist in the oil,
clean the oil from the condenser lens and bottom of slide with a
camera cleaning paper and repeat the procedures.
5) Follow the procedures in this manual to bring the sample on slide
in focus and observe.
6) When using the 100X oil darkfield objective, you will need to drop
the immersion oil and adjust the iris ring (Fig. 14) to get proper
brightness and contrast of view field.
Note：
The condenser won’t work well if no oil drop applied on the condenser
8. Phase Contrast Kit Installation and Operation
Instructions
8.1 Phase contrast kits of A1PHB1 or A1PHB3:
1) Mounting the phase contrast objectives
a. Take off all the objectives from the nosepiece.
b. Install the phase contrast objectives onto the nosepiece following the steps in 2.3.
2) Mounting the phase contrast condenser/annular ring disk
a. Rotate the nosepiece and set the 4X objective in position.
b. Turn the coarse focus knob to raise the mechanical stage to the highest position
without contacting the 4X objective.
c. Turn the condenser control knob to raise the condenser to the highest position.
d. Loosen the light collector and take it off.
e. Loosen the condenser lock thumb screw, pull down
the condenser and take it off.
f. Insert the condenser/annular ring disk into the
condenser holder, and tighten the thumb screw.
g. Insert the light collector back onto the base and turn
until it’s tightened.
h. Re-adjust the condenser height to its normal
position.
i. Re-adjust the mechanical stage height to its normal
position.
Note:
When raising the mechanical stage, do not make
contact with the objective.
3) Turn the desired objective into light path.
4) Turn the annular ring disk to put the corresponding
ring into light path, i.e. if you are using the 20X
phase contrast objective, you should turn the disk at
Fig.14
Iris Ring
20
Fig.15
Fig.16

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(a) (b)
(c) (d)
20 as shown in Fig. 15.
5) Centering the annular ring.
a. Remove one eyepiece from the microscope eyepiece
tube and insert the centering telescope as shown in
Fig. 16.
b. Observe from the telescope. The bright ring and dark
ring should be coincided with each other as shown in
Fig.17 (d).
c. If the ring images are not clear, turn the top of
telescope until both ring images are in focus.
d. If the bright ring is still obscure as in Fig.17 (b), adjust
the condenser focus knob.
e. If the two ring images are not coincided as shown in
Fig.17 (c), hold the ring plate from the bottom of the
annular ring disk and adjust its position until two ring
images are coincided.
f. Remove the centering telescope and replace it with the
eyepiece.
g. Put the specimen on the stage and adjust the illumination, focusing, etc following the
instructions in this manual.
Note:
The phase contrast condenser will be working as a conventional Abbe condenser if
the annular ring disk being put at Bposition.
6) Performing the phase contrast observation.
After centering the condenser ring plate, perform the phase contrast observation the
same way as a normal bright field microscope.
Note:
When change to another phase contrast objective and corresponding condenser
ring plate, the focusing and centering of bright ring and dark ring should be repeated
following the procedures from 5)-b to 5)-f.
8.2 Phase contrast kit of A1PHC2:
1) Mounting the phase contrast kit
a. Replace the brightfield objective(s) on nosepiece with the phase contrast
objective(s).
b. Rotate the nosepiece and set the 4X objective in position.
c. Turn the coarse focus knob to raise the mechanical stage to the highest position
without contacting the 4X objective.
d. Turn the condenser control knob to raise the condenser to the highest position.
e. Loosen the condenser lock thumb screw (Fig. 18), pull down the condenser and
take it off.
f. Insert the condenser/annular ring disk into the condenser holder, and tighten the
thumb screw (Fig. 18).
g. Re-adjust the condenser height to its normal position.
h. Re-adjust the mechanical stage height to its normal position.
Fig.17

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18
Condenser Holder
Condenser Lock Thumb Screw
Note:
There are 2 phase contrast objectives: 10X and 40X, and there are 2 condenser
annular rings on the sliding selector: 10/20 and 40. The corresponding objective,
annular ring and iris diagram must work together, i.e., 40Xphase contrast objective
must work with 40 condenser annular ring and iris diagram 40 and so on.
2) Centering the condenser ring plate
a. Connect the power cord to the microscope and
insert the plug into a power outlet.
b. Turn the desired phase contrast objective into light
path. Slide the sliding selector to the corresponding
annular ring and turn iris diagram corresponding to
the phase contrast objective in the light path.
c. Remove one eyepiece from the microscope
eyepiece tube and insert the centering telescope.
d. Observe from the telescope. The bright ring and
dark ring should be coincided with each other as
shown in Fig. 19 (d).
e. If the ring images are not clear, turn the top of
telescope until both ring images are in focus.
f. If the bright ring is still obscure as in Fig. 19 (b),
adjust the condenser focusing knob.
g. If the two ring images are not coincided as shown in Fig. 19 (c), adjust the two
centering screws on the phase contrast condenser.
h. Remove the centering telescope and replace the eyepiece.
3) Performing the phase contrast observation
Perform the phase contrast observation the same way as a normal brightfield
microscope.
Note:
When change to another phase contrast objective and corresponding condenser
ring plate, the focusing and centering of bright ring and dark ring should be repeated
following the procedures from 2)-b to 2)-h.
4) Performing the darkfield observation
a. Slide the sliding selector to the darkfield position, the condenser will work as a
darkfield condenser.
b. Turn iris diagram to 100.
c. Perform the darkfield observation the same way as a normal brightfield microscope.
Fig.18
(a) (b)
(c) (d)
Fig. 19