Opentrons GEN1 User manual

Heater-Shaker Module GEN1
Instruction Manual
Opentrons Labworks Inc.
Edition
GEN1, August 2022

–SafeUseSpecications
– LED Status Light
–SafetyWarningLabels
–StandardsCompliance
Table of Contents
Product and Manufacturer Description 3
Safety Information and Regulatory Compliance 4
–ProductDescription
–ManufacturerDescription
–ModelNumber
–IncludedParts
–ThermalAdapters
–PhysicalSpecications
–PowerConsumption
–ShakingProle
–TemperatureProle
–LiquidClassesandVolumes
–SupportedLabware
Product Specications 10
–In-appSetupGuide
– Setup Steps
–SoftwareControl
–Maintenance
Setup and Maintenance 13
–OpentronsHelpCenter
–ContactOpentrons
–Warranty
Support 21

3
Product and Manufacturer Description
PRODUCT DESCRIPTION
TheOpentronsHeater-ShakerModuleGEN1provideson-deckheating
andorbitalshaking.Themodulecanbeheatedto95°C,andcan
shakesamplesfrom200to3000rpm.
MANUFACTURER DESCRIPTION
Opentrons Labworks Inc
45-18CtSquareW
LongIslandCity,NY11101

HEATER-SHAKER MODULE GEN1
Safety Information and Regulatory Compliance
Opentronsstronglyrecommendsthatyoufollowthesafeuse
specicationslistedinthissectionandthroughoutthismanual.
The shaking motion of the Heater-Shaker requires additional
anchoring — more than simply clipping it into a deck slot — to
ensure that it does not dislodge itself from the deck of your
robot. See the Setup Steps section of this manual or the on-
screen instructions in the Opentrons App for details on how to
anchor the module.
SAFE USE SPECIFICATIONS
Input and Output Connections
TheHeater-Shakerhasthefollowingpowerinputandoutput
requirements,whicharemetbytheincludedpowersupplybrick.
Warning:Donotreplacethepowersupplycordunlessatthe
directionofOpentronsSupport.
PowerSupply(AC):
◾Voltage:100–240V
◾Frequency:50/60Hz
◾Current:4.0A
◾Mains supply voltage uctuation: ±10%
◾Overvoltage:CategoryII
Heater-Shaker(DC):
◾Voltage: 36V
◾Current:6.1A
Theseinputandoutputrequirementsarealsoindicatedonthe
externalpoweradapter.

5
Safety Information and Regulatory Compliance
Environmental Conditions
Foroptimalperformance,Opentronsrecommendsusingthe
Heater-Shakerinthefollowingconditions:
◾Environment:Indooruseonly
◾Temperature: 20–25°C
◾Relative humidity: 80%maximum,non-condensing
◾Altitude: Upto2,000metersabovesealevel
◾Pollution degree: 2
TheHeater-Shakercanbesafelytransportedandstoredat
temperaturesof−10to60°C.
Software Requirements
TheHeater-Shakerrequiresversion6.1.0orneweroftheOpentrons
AppandOT-2robotserver.
YoucandownloadtheOpentronsAppforWindows,macOS,or
Ubuntuathttps://opentrons.com/ot-app/.

HEATER-SHAKER MODULE GEN1
Safety Information and Regulatory Compliance
LED STATUS LIGHT
TheHeater-ShakerhasanLEDstatuslightthatcandisplaythreecolors:
amber,red,andwhite.Readingthesestatusindicationsisimportantfor
safehandlingofthemodule(seeTemperatureSafetybelow).Thepossible
moduleconditionsindicatedbythelightare:
LED COLOR AND BEHAVIOR MODULE CONDITIONS
Pulsingred Moduleisheating
Solidred Moduleisholdingattarget
temperature
and/or
Moduleishottothetouch(>49°C)
Solidwhite Moduleisonandidle
and/or
Modulehasbeendeactivatedandit
isnothottothetouch(<49°C)
Pulsingwhite Moduleisinidentifymode(to
distinguishbetween
multipleHeater-ShakerModules)
Pulsingamber Moduleisinanerrorstate
Pulsingredandamber,
alternating
Moduleisinanerrorstate
and
Moduleishottothetouch(>49°C)
Pulsingredandwhite,
alternating
Moduleisinidentifymode
and
Moduleisheating
LEDo Moduleispoweredo

7
Safety Information and Regulatory Compliance
Temperature Safety
Duringnormaloperation,thetopplate,labwareadapters,and
labwareontopoftheHeater-Shakercanreachtemperatures of up
to 95 °C.Touchingthesesurfaceswhiletheyarehotposestheriskof
burns.
Toreducetheriskofburns,alwayscheckthecolorofthestatuslight
beforetouchingthemodule.Ifthestatuslight is red,themodulemay
behottothetouch.Do not touchthemodule.UsetheOpentronsApp
todeactivatetheheaterandallowthemoduletocooluntilthestatus
lightissolidwhite.
Ifyoumusttouchamodulethatispoweredo,andyouareunsure
whetheritishottothetouch:
◾Ifyoucandosowithout touching any other part of the module,
pressthepowerbuttontoactivatethemoduleandcheckthe
colorofthestatuslight.
◾Ifyoucannotsafelypressthepowerbutton,allowthemoduleto
coolforatleast10minutesbeforetouchingit.
Powering Down
WhenpoweringdowntheHeater-ShakerModule,ensurethatitisidle
(notshaking)andnothottothetouchbycheckingthatitsstatuslight
issolidwhite.Ifthestatuslightisred,themoduleishot;allowthe
moduletocooluntilthestatuslightiswhitebeforepoweringitdown.
Poweringdownthemodulewhilethestatuslightisredpreventsother
usersfromdeterminingwhetherthemoduleishottothetouch.
PressthepowerbuttonabovetheUSBconnectortoturnothe
module.Youcanunplugthepoweradapteroncethemoduleis
powereddown.

HEATER-SHAKER MODULE GEN1
Safety Information and Regulatory Compliance
SAFETY WARNING LABELS
WarninglabelsontheHeater-Shakerindicatesourcesofpotential
injuryorharm.
Hot Surface: This symbol identies instrument
components that, if handled improperly, pose a risk
of personal injury due to high temperature (>49 °C).
STANDARDS COMPLIANCE
TheHeater-Shakerhasbeentestedandfoundtobeincompliance
withallapplicablerequirementsofthefollowingsafetyand
electromagneticstandards:
Safety
◾IEC/EN61010-1SafetyRequirementsforElectricalEquipmentfor
Measurement,ControlandLaboratoryUse
◾IEC61010-2-010RequirementforHeating
◾IEC61010-2-051:2018Particularrequirementsforlaboratory
equipmentformixingandstirring
Electromagnetic Compatibility
◾FCCpart15subpartBclassA
◾IEC/EN61326-1EMCTestingofLaboratoryEquipment
Hazardous Substances: RoHScompliant

9
Safety Information and Regulatory Compliance
FCC Warnings and Notes
Warning:Changesormodicationstothisunitnotexpressly
approvedbyOpentronsLabworksInc.couldvoidtheuser’sauthority
tooperatetheequipment.
Note:Thisequipmenthasbeentestedandfoundtocomplywiththe
limitsforaClassAdigitaldevice,pursuanttopart15oftheFCCRules.
Theselimitsaredesignedtoprovidereasonableprotectionagainst
harmfulinterferencewhentheequipmentisoperatedinacommercial
environment.Thisequipmentgenerates,uses,andcanradiateradio
frequencyenergyand,ifnotinstalledandusedinaccordancewith
theinstructionmanual,maycauseharmfulinterferencetoradio
communications.Operationofthisequipmentinaresidentialarea
islikelytocauseharmfulinterferenceinwhichcasetheuserwillbe
requiredtocorrecttheinterferenceathisownexpense.
Note regarding FCC compliance:Althoughthisinstrumenthas
beentestedandfoundtocomplywithPart15,SubpartBoftheFCC
RulesforaClassAdigitaldevice,pleasenotethatthiscomplianceis
voluntary,fortheinstrumentqualiesasan“exempteddevice”under
47CFR15.103(c),inregardtothecitedFCCregulationsineectatthe
timeofmanufacture.
CISPR 11 Class A
Caution: Thisequipmentisnotintendedforuseinresidential
environmentsandmaynotprovideadequateprotectiontoradio
receptioninsuchenvironments.

HEATER-SHAKER MODULE GEN1
Product Specifications
MODEL NUMBER
GEN1
INCLUDED PARTS
◾(1)PowerSupply
◾(1)PowerCable
◾(1)USBCable
◾(1)T10TorxScrewdriver
◾(1)T10TorxLKey
◾(1)M3x7ThermalAdapterScrew(plusspares)
THERMAL ADAPTERS
Acompatiblethermaladapterisrequiredforaddinglabwaretothe
Heater-ShakerModule.Adapterscanbepurchaseddirectlyfrom
Opentronsathttps://shop.opentrons.com.
CurrentlyavailableThermalAdaptersinclude:
◾UniversalFlatAdapter
◾PCRAdapter
◾DeepWellAdapter
◾96FlatBottomAdapter
PHYSICAL SPECIFICATIONS
Allspecicationsareforthemoduleasshipped:withlatchesinthe
restpositionandwithoutlabwareoradapters.
◾Dimensions:152mmLx90mmWx82mmH
◾Weight:1.34kg
◾Composition:CNCaluminumandpolycarbonateplastic

11
Product Specifications
POWER CONSUMPTION
Thesearetypicalpowerconsumptionvaluesmeasuredatthewall
outlet.Typicalpowerconsumptioniswhenthemoduleismaintaining
temperaturesandshakespeedsinthenormalrange.Themaximum
powerconsumptioniswhenthemoduleissimultaneouslyheatingat
fullpowerandshakingatmaximumspeed.
Idle:3W
Typicalconsumption:
◾Shaking: 4–11 W
◾Heating:10–30W
◾Heating and shaking: 10–40 W
Maximumconsumption:125–130W
SHAKING PROFILE
◾Orbital diameter:2.0mm
◾Orbital direction:Clockwise
◾Speed range:200–3000rpm(somelabwaremayrecommend
alowermaximumrpm)
◾Speed accuracy:±25rpm
TEMPERATURE PROFILE
◾Temperature range: 37–95°C
◾Temperature accuracy: ±0.5°Cat55°C
◾Temperature uniformity: ±0.5°Cat55°C
◾Ramp rate: 10°C/min

HEATER-SHAKER MODULE GEN1
Product Specifications
LIQUID CLASSES AND VOLUMES
Forfurtherinformation,consultthemostrecentapplicationnotes
andwhitepapersavailableontheHeater-Shakerproductpageon
theOpentronswebsite.
SUPPORTED LABWARE
◾NEST96Well,0.1mL,RoundWell,VBottom,FullSkirt
◾NEST96Well,0.2mL,RoundWell,FlatBottom,FullSkirt
◾NEST96Well,DeepWell,2mL,SquareWell,VBottom,FullSkirt
◾ThermoScienticArmadilloPCRPlate,96-Well,Clear,ClearWells
◾Corning384WellPlate112µLFlat
Customat-bottomlabwarecanbeusedwiththeUniversalFlat
Adapter.Ifyouneedassistancecreatingcustomlabwaredenitions
fortheHeater-Shaker,submitarequest.

13
Setup and Maintenance
IN-APP SETUP GUIDE
Forinteractivesetupinstructionsandtorunatestshakewithyour
Heater-ShakerModule,connectthemoduletoyourrobot(see
ConnectingUSB&Powerbelow)andeither:
◾GotothePipettes and Modulessectionontherobot’sdevice
detailpage,or
◾LoadaprotocolthatrequiresthemoduleandgotoStep 2:
Module SetupintheSetuptaboftheprotocolrunpage.
SETUP STEPS
Preparing the Module
Orient the module so its ports face away from you.
1
Make sure the anchors are not extended and are level
with the module’s base. Turn the screws above each
anchor counterclockwise to retract the anchors. The
screws should not come out of the module.
2

HEATER-SHAKER MODULE GEN1
Setup and Maintenance
Attaching to Deck
Choose which slot you will place the Heater-Shaker in.
Slots 1, 3, 4, 6, 7, and 10 are supported.
3
Place the module in the slot such that the power and
USB ports are facing outward, i.e. to the left in slot 1, 4,
7, or 10; to the right in slot 3 or 6.
4

15
Hold the module at against the deck and turn the
screws clockwise to extend the anchors.
5
Check that the module is rmly attached by gently
pulling up on it and rocking it from side to side.
6
Setup and Maintenance

HEATER-SHAKER MODULE GEN1
Use the included T10 Torx Screwdriver
and Thermal Adapter Screw to attach
your chosen adapter to the module.
7
Using a dierent
screwdriver can strip
the screws. Using
dierent screws can
damage the module.
IMPORTANT:
Check the alignment of the thermal adapter.
If properly attached, it will sit evenly on the module.
8
Check that the adapter is rmly attached by
rocking it back and forth.
9
Setup and Maintenance
Attaching a Thermal Adapter

17
Power on the robot and module, and launch the
Opentrons App.
11
Ifyousuccessfullyconnectedyourmodule,itwillappear
inthePipettes and Modulessectiononyourrobot’s
devicedetailpage.Fromthereyoucancontrolthe
module’slabwarelatchorrunatestshake.
Setup and Maintenance
Connecting USB & Power
The module requires two connections:
10
1. Connectthepowercabletothepowersupplyand
themodule.Routethecablethroughthegapatthe
bottomoftherobot’ssidewindow.
2. ConnecttheUSBcabletothemoduleandanopen
USBportatthebackoftherobot.Usethecable
holdersonthesideoftherobottokeeptheUSBcable
outoftheway.

HEATER-SHAKER MODULE GEN1
Setup and Maintenance
SOFTWARE CONTROL
TheHeater-ShakerModulecanbecontrolledinJSON(Protocol
Designer)andPythonprotocols.Runningtheseprotocols
requiresversion6.1.0orneweroftheOpentronsAppandOT-2
robotserver.
TheOpentronsAppdisplaysthecurrentstatusoftheHeater-
Shakerandcanalsocontrolthemoduleoutsideofprotocols.
TocontrolaHeater-Shaker,gototheDevicestabandselect
therobotthatitisconnectedto.Robotswithaconnectedand
poweredonHeater-Shakerwillhavea iconundertheModules
sectionofthedevicecard.
Onthedevicedetailpage,amodulecardshowsthecurrent
statusoftheHeater-Shaker,including:
◾Whethertheheaterisheating,cooling,orholdingattarget.
Awarningbannerwillappearifthemoduleishottothe
touch(>49°C).
◾Thetarget(ifset)andcurrenttemperature
◾Whethertheshakerisspeedingup,slowingdown,or
holdingattarget
◾Thetarget(ifset)andcurrentshakespeed
◾Whetherthelabwarelatchisopenorclosed

19
Setup and Maintenance
Clickthethree-dotmenutorevealtheavailablecontrols
fortheHeater-Shaker.Youcancontroltheheater,shaker,and
labwarelatchindependently.Youcanalsoviewinformation
aboutthemodule,includingitsserialnumberandrmware
version,orlaunchthein-appattachmentguide.

HEATER-SHAKER MODULE GEN1
Setup and Maintenance
MAINTENANCE
Device Lifetime
TheHeater-Shakerhasbeentestedfor3yearsoffull-timeoperation
(morethan1000totalhours)withoutmaintenance.
Service and Repair
Usersshouldnotattempttoserviceorrepairthemodulethemselves.
Ifyouhaveconcernsaboutyourmodule’sperformanceorrequire
service,pleasecontactOpentronsSupport.
Cleaning
BeforecleaningtheHeater-Shaker,unplugthepowerandUSBcables
andremoveitfromthedeck.
AlloutersurfacesoftheHeater-Shakerarecompatiblewith:
◾10%bleachsolution
◾70%ethanol
◾99%isopropylalcohol
Sprayoneofthesecleanersontoapapertowelanduseittowipethe
exteriorsurfacesofthemodule.Avoidintroducingthesesubstances
intotheUSBportorpowerport.Neverdouseorspraythemodule
directlywithcleaner.
Themodulecanalsowithstandultraviolet(UV)light,limitedto
standardsterilizationprocedures.Donotexposethemoduleto
constantorhigh-intensityUVlight.