Opentrons GEN1 User manual

Opentrons Labworks Inc.
September 2023
Heater-Shaker Module GEN1
Quickstart Guide

Post-sales service & contacting Opentrons
If you have any questions about the use of the system,
abnormal phenomena, or special needs, please contact:
[e-mail]. Also visit www.opentrons.com.
Product Description
The Opentrons Heater-Shaker Module provides on-deck heating and
orbital shaking. The module can be heated to 95 °C, and can shake
samples from 200 to 3000 rpm. The Heater-Shaker is compatible with
the Opentrons Flex and Opentrons OT-2 liquid handling robots.

Table of Contents
Product Elements 4
Before You Begin 7
– Deck Placement and Cable Alignment
– Anchor Adjustments
Flex Attachment Steps 10
OT-2 Attachment Steps 11
Attaching the Thermal Adapter 12
Additional Product Information 14
– Maintenance
– Warranty
– Support
– App Download
–CompletedCertications
– Recommended Operating Conditions
– Manufacturer Description
– Box Contents
– Thermal Adapters
– Flex Caddies

HEATER-SHAKER MODULE GEN1 4
Product Elements
Labware Latch
Power Button
Serial Number
Power Port
USB Port
2x Anchors
Heating &
Shaking Plate
BOX CONTENTS
(1) Power Supply
POWER SUPPLY
IEC CONNECTOR
(1) Power Cable (1) USB Cable
TORX SCREW DRIVER
(1) T10 Torx Screwdriver
THERMAL ADAPTOR SCREW
(1) M3x7 Thermal Adapter Screw
(plus spares)
(1) T10 Torx L Key

HEATER-SHAKER MODULE GEN1 5
The Heater-Shaker requires a thermal adapter to hold your labware.
You can purchase adapters directly from Opentrons at
shop.opentrons.com.
THERMAL ADAPTERS
Universal Flat Adapter
UNIVERSAL FLAT PLATE ADAPTOR
PCR PLATE ADAPTOR
DEEP WELL PLATE ADAPTOR
ROUND BOTTOM PLATE ADAPTOR
PCR Adapter
Deep Well Adapter Flat Bottom Adapter

HEATER-SHAKER MODULE GEN1 6
FLEX CADDIES
WhenusedwithaFlexrobot,theHeater-Shakertsintoacaddythat
occupies space below the deck. The caddy places your labware closer
to the deck surface and allows for below-deck cable routing. See the
Modules chapter in the Flex Instruction Manual for more information.
The OT-2 does not use caddies. Modules clip directly to the deck.
Module caddies are available for purchase at shop.opentrons.com.
Handle
On/Off switch

HEATER-SHAKER MODULE GEN1 7
Before You Begin
The supported deck slot positions for the Heater-Shaker depend on the
robot you’re using.
Robot model Deck placement
Flex In any deck slot in column 1 or 3. The module
can go in slot A3, but you need to move the
trashbinrst.
OT-2 In deck slot 1, 3, 4, 6, 7, or 10.
DECK PLACEMENT AND CABLE ALIGNMENT
Review this section for important information about Heater-Shaker
deck placement, alignment, and anchor adjustments before installing
the module.
OT-2Flex

HEATER-SHAKER MODULE GEN1 8
Robot model Power and USB alignment
Flex ◾Facing left in column 1.
◾Facing right in column 3.
OT-2 ◾Facing left in slot 1, 4, 7, or 10.
◾Facing right in slot 3 or 6.
Do not install the Heater-Shaker with the ports facing in, towards the
middle of the deck. This alignment makes cable routing and access
difcult.
To properly align the Heater-Shaker relative to the robot, make sure the
module’s power and USB ports face outward, away from the center of
the deck. This helps make cable routing and access easier.

HEATER-SHAKER MODULE GEN1 9
Before installation:
◾Check the anchors to make sure they’re level with the base of the
Heater-Shaker.
◾If the anchors interfere with installing the module, adjust them
until there’s enough clearance to seat the module and then tighten
them to hold it in place.
ANCHOR ADJUSTMENTS
Anchors are screw-adjustable panels on the bottom of the Heater-
Shaker. They provide the clamping force that secures the module to
its caddy or deck slot and prevents it from vibrating loose. Use the T10
Torx screwdriver to adjust the anchors.
◾To loosen/retract the anchors, turn the screws counter-clockwise.
◾To tighten/extend the anchors, turn the screws clockwise.

HEATER-SHAKER MODULE GEN1 10
Flex Attachment Steps
To attach the Heater-Shaker to your Flex:
1. Choose the supported slot you want to use for the module. Use the
2.5 mm screwdriver to remove the deck slot plate.
2. Insert the Heater-Shaker into its caddy by aligning the power
button on the module with the on/off switch on the caddy.
3. Holding the module in the caddy, use the T10 Torx screwdriver to
turn the anchor screws clockwise and extend the anchors. The
module is secure when it doesn’t move while gently pulling on it
and rocking it from side to side.
4. Connect the power and USB cables to the module.
5. Insert the caddy into the deck slot and route the power and USB
cables through the removable side covers of the Flex. Do not
connect the power cable to a wall outlet yet.
6. Connect the other end of the USB cable to a USB port on the Flex.
7. Connect the power cable to a wall outlet. Gently press the on/off
switch to turn the module on.
8. If you successfully connected the module, it will appear in the
Pipettes and Modules section on your robot’s device detail page
in the Opentrons App. From there, you can control the module’s
labware latch or run a test shake.
Tip: If you’re trying to force a Heater-Shaker into a caddy,
the module’s power button is probably facing away from
the caddy’s on/off switch. Turn the module so the power
button faces the on/off switch and try again.

HEATER-SHAKER MODULE GEN1 11
OT-2 Attachment Steps
To attach the Heater-Shaker to your OT-2:
1. Choose the supported slot you want to use for the module and
press it gently into place.
2. Holdingthemoduleatagainstthedeck,usetheT10Torx
screwdriver to turn the anchor screws clockwise and extend the
anchors. The module is secure when it doesn’t move while gently
pulling on it and rocking it from side to side.
3. Connect the USB cable to the module and then connect the other
end of the USB cable to a USB port on the OT-2. Use the cable
holders on the side of the robot to keep the USB cable out of the
way.
4. Connect the power cable to the module and then to a wall outlet.
Gently press the on/off switch to turn the module on.
5. If you successfully connected the module, it will appear in the
Pipettes and Modules section on your robot’s device detail page
in the Opentrons App. From there, you can control the module’s
labware latch or run a test shake.
Warning: The shaking motion of the Heater-Shaker
Module requires additional anchoring — more than
simply clipping it into a deck slot — to ensure that it
does not dislodge itself from the deck of your OT-2.

HEATER-SHAKER MODULE GEN1 12
Attaching the Thermal Adapter
1. Use the T10 Torx screwdriver and thermal adapter screw to
fasten your adapter to the module.
You can attach a thermal adapter before or after installing the
Heater-Shaker. The module latches must be open before installing
the thermal adapter. You can open the latches via the module card
for the Heater-Shaker in the Opentrons App.
To attach a thermal adapter to the module:
Warning: Using a different screwdriver can strip the
screw. Using a different screw can damage the module.

HEATER-SHAKER MODULE GEN1 13
2. Check the alignment of the thermal adapter. The adapter sits
evenly on the module when attached properly.
3. Checkthattheadapterisrmlyattachedbyrockingitbackand
forth. It’s secure if it doesn’t move.

HEATER-SHAKER MODULE GEN1 14
Additional Product Information
The Heater-Shaker Module has been tested for 3 years of full-time
operation without maintenance. Users should not attempt to service
or repair the module themselves. If you have concerns about your
module’s performance or require maintenance, please contact
Opentrons Support.
MAINTENANCE
All hardware purchased from Opentrons is covered under a 1-year
standard warranty. Opentrons warrants to the end-user of the products
that they will be free of manufacturing defects due to part quality
issues or poor workmanship and also warrants that the products will
materiallyconformtoOpentrons’publishedspecications.
WARRANTY
Opentrons Support can help you with questions about our products
and services. If you discover a defect, or believe your product is not
functioningtopublishedspecications,contactusat
[e-mail].
Please have the Heater-Shaker’s serial number available when
contactingsupport.Youcanndtheserialnumberonthebottomof
the module or in the Opentrons App. On the Heater-Shaker card in the
Pipettes and Modules section of your robot’s device detail page, click
the three-dot menu (⋮) and then About.
SUPPORT
Control your liquid handling robot and module using the Opentrons
App. Download the app for Windows, macOS, or Ubuntu at
https://opentrons.com/ot-app/.
APP DOWNLOAD

HEATER-SHAKER MODULE GEN1 15
IEC, FCC, RoHS
COMPLETED CERTIFICATIONS
Environmental temperature: 20–25 °C
Environmental humidity: 80% maximum
RECOMMENDED OPERATING CONDITIONS
Opentrons Labworks Inc
45-18 Ct Square W
Long Island City, NY 11101
MANUFACTURER DESCRIPTION

For more information, download the complete
Opentrons Heater-Shaker Module GEN1 Instruction Manual
from the Opentrons Knowledge Hub.