Optica B-150 Series User manual
Model
B-150 series (B-151 / B-153 / B-155 / B-157 / B-159)
B-150 ALC series (B-151ALC / B-153ALC / B-155ALC / B-157ALC / B-159ALC)
B-150R-PL series (B-151R-PL / B-152R-PL / B-153R-PL / B-155R-PL / B-157R-PL / B-159R-PL)
B-150 Series
INSTRUCTION MANUAL
Ver. 12.1 2022
Page 2
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 B-151 / B-151ALC / B-151R-PL 4
3.2 B-152R-PL / B-153 / B-153ALC / B-153R-PL 4
3.3 B-155 / B-155ALC / B-155R-PL 5
3.4 B-157 / B-157ALC / B-157R-PL 5
3.5 B-159 / B-159ALC / B-159R-PL 6
4. Unpacking 7
5. Intended use 7
6. Symbols and conventions 7
7. Instrument description 8
7.1 B-151 / B-151R-PL 8
7.2 B-153 / B-155 / B-157 / B-159 / B-152R-PL / B-153R-PL / B-155R-PL / B-157R-PL / B-159R-PL 9
7.3 B-151ALC / B-153ALC / B-155ALC / B-157ALC / B-159ALC 10
8. Assembling 11
8.1 Assembling the microscope 11
8.2 Polarizing set (optional) 12
9. Use of the microscope 13
9.1 Light intensity adjustment 13
9.2 Use of ALC system 13
9.3 Coarse focus tension adjustment 13
9.4 Stage 13
9.5 Adjust the interpupillary distance 14
9.6 Diopter adjustment 14
9.7 Use of oil immersion objective 14
9.8 Aperture diaphragm 15
9.9 Use with rechargeable batteries 15
9.10 Use of the polarizer (optional) 15
10. Maintenance 16
11. Troubleshooting 17
Equipment disposal 18
Table of contents
Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.
Page 4
3. Package content
3.1 B-151 / B-151ALC / B-151R-PL
3.2 B-152R-PL / B-153 / B-153ALC / B-153R-PL
① Frame
② Monocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
③
④
⑤
⑥⑦
⑧
①
②
① Frame
② Monocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives
• B-152R-PL(4X/10X/40X)
• B-153(all)(4X/10X/40X/60X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
③
④
⑤
⑥⑦
⑧
①
②
Page 5
3.3 B-155 / B-155ALC / B-155R-PL
3.4 B-157 / B-157ALC / B-157R-PL
① Frame
② Monocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X/100X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
⑨ Immersionoil
③
④
⑤
⑥⑦
⑧
①
②
⑨
① Frame
② Binocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X/60X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
③
④
⑤
⑥⑦
①
②⑧
Page 6
3.5 B-159 / B-159ALC / B-159R-PL
① Frame
② Binocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X/100X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
⑨ Immersionoil
③
④
⑤
⑥⑦
⑧
①
②
⑨
Page 7
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
Page 8
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
EYEPIECE
OBSERVATION
HEAD
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
(ONLY “R”
VERSION)
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
STAGE CLIPS
7. Instrument description
7.1 B-151 / B-151R-PL
Page 9
TENSION
ADJUSTMENT
COLLAR
SLIDE
HOLDER
LED CHARGE
INDICATOR
(ONLY “R”
VERSION)
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
STAGE
OBSERVATION HEAD
-) MONOCULAR (B-152 / B-153 / B-155)
-) BINOCULAR (B-157 / B-159)
CONDENSER
OBJECTIVES
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
NOSEPIECE
X/Y MOVEMENT
KNOBS
EYEPIECE
7.2 B-153 / B-155 / B-157 / B-159 / B-152R-PL / B-153R-PL / B-155R-PL / B-157R-PL / B-159R-PL
Page 10
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
ALC ON/OFF BUTTON
ALC CONNECTION CABLE
7.3 B-151ALC / B-153ALC / B-155ALC / B-157ALC / B-159ALC
Page 11
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
FFig. 4ig. 4
8. Assembling
8.1 Assembling the microscope
1. Remove the dust cap from the microscope frame and from
the bottom of the observation head.
2. Insert the optical head above the stand and tighten the screw.
(Fig. 1)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
Only for ALC models:
3. Connect the cable of the ALC (Automatic Light Control) sys-
tem to the socket placed on the observation head and on the
back side of the frame. (Fig. 2)
4. Insert both eyepieces into the tubes of the optical head. (Fig.
3)
• For models B-151, B-152, B-153 and B-155: only one eye-
piece is used as these models are monocular.
5. Insert the power supply jack in the socket placed at the rear
side of the microscope. (Fig. 4)
Page 12
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
①
②
③
8.2 Polarizing set (optional)
1. Place the polarizer on the light exit ① at the base of the mi-
croscope. (Fig. 5)
2. Loosen the head xing knob ② and remove the head from
the microscope frame. (Fig. 6)
3. Insert the analyzer ③ into the hole inside the frame. (Fig. 7)
4. Put back the head into its original position and lock the xing
knob.
Page 13
①
②
FFig. 8ig. 8
FFig. 9ig. 9
FFig. 10ig. 10
FFig. 11ig. 11
④
③
9. Use of the microscope
9.1 Light intensity adjustment
Operate on the light intensity adjustment dial ① to turn ON /
OFF the microscope and to increase / decrease the illumination
voltage. (Fig. 8)
9.2 Use of ALC system
1. Press the ALC button ② (Fig. 9). The light on the microscope
will turn o for some seconds, the will turn on again.
2. An optimal brightness level is set
3. Now the system will automatically adapt the brightness to the
eyepieces when an objective is changed, when the aperture
diaphragm is used or when another specimen is placed on
the stage.
4. Pressing the ALC button again, the ALC system will be dis-
abled.
• When ALC system is active the light intensity dial is not
active.
9.3 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-set in the factory.
1. To modify the tension according to personal’s needs, rotate
the ring ③ using the provided tool (Fig. 10).
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension.
9.4 Stage
All models except B-151 series
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverslide 0,17mm. (Fig. 11)
1. Open the spring arm of the slide holder ④ and place the slide
from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the fall-
ing of the slide.
Page 14
FFig. 12ig. 12
②
①
FFig. 13ig. 13
FFig. 14ig. 14
9.5 Adjust the interpupillary distance
• This operation can be done only on binocular models.
1. Hold the right and left parts of the observation head using
both hands and adjust the interpupillary distance by turning
the two parts until one circle of light can be seen. (Fig. 12)
• The graduation on the interpupillary distance indicator ①,
pointed by the spot “.” on the eyepiece holder, shows the
distance between the operator’s eyes.
The range of the interpupillary distance is 48- 75 mm.
9.6 Diopter adjustment
• This operation can be done only on binocular models.
1. Look into the right eyepiece with your right eye only, and fo-
cus on the specimen.
2. Look into the left eyepiece with your left eye only. If the image
is not sharp, use the diopter adjustment ring ②to compen-
sate. (Fig. 13)
• The adjustment range is ±5 diopter. The number indicat-
ed on the adjustment ring graduation should correspond
to the operator’s diopter correction.
9.7 Use of oil immersion objective
B-155 and B-159 models only
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 14)
• Make sure that there are no oil bubbles. Air bubbles in
the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully open the ap-
erture diaphragm and observe the objective exit pupil. (The
pupil must be circular and bright).
• To remove the bubbles, gently move the nosepiece to the
right and left to move the immersion objective a few times
and allow the air bubbles to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and obtain an
optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper towel or a
lightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute ethyl alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the specimen would
be dicult (even not impossible) due to the presence of
an additional thickness on the objective.
Page 15
FFig. 15ig. 15
①
FFig. 16ig. 16
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 17ig. 17
②
9.8 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture dia-
phragm aects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of
focus of the image. Move the diaphragm lever ①(Fig. 15)
toward left or right to decrease or increase the N.A. value.
• With low contrast specimens set the numerical aperture to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the con-
denser’s diaphragm in order to obtain an image like the one
in Fig. 16.
9.9 Use with rechargeable batteries
This operation can be done only with “R” models.
When the microscope is plugged with the power supply, the LED
indicator for the battery recharge ② is lit. (Fig. 17)
• LED red: battery under charge
• LED green: battery fully charged.
When the microscope is unplugged, the LED is o.
During the normal use with batteries, LED is always OFF.
9.10 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer until the
darkest position is achieved.
3. Once the dark is achieved (“extinction” or “Crossed Nicol”
position) it is possible to begin the observation.
Page 16
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of 5°-40°C and
a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o the light immediately, cover the microscope with the provided dust-cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in o-position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sucient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or near
electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.
10. Maintenance
Page 17
11. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Batteries are uncharged Fully charge the batteries
Dirt or dust is visible in the eld of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped down
too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an incorrect
position
Move the nosepiece to a click stop
Aperture diaphragm is too closed or to
open
Adjust aperture diaphragm
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly
One side of the image is out of focus. The nosepiece is not in the center of
the light path Turn the nosepiece to a click stop
The specimen is out of place (tilted) Place the specimen at on the stage.
The optical performance of the sample
cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to turn. The tension adjustment collar is too
tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is too
loose Tighten the tension adjustment collar
III. Electric section:
The LED doesn’t turn on. No power supply Check the power cord connection
Batteries are uncharged Fully charge the batteries
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly connected Check the power cord
IV. Observation tube:
Field of view of one eye does not match
that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to micro-
scope observation.
Upon looking into eyepieces, try look-
ing at overall field before concentrating
on specimen range. You may also find
it helpful to look up and into distance
for a moment before looking back into
microscope.
Page 18
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating to the reduc-
tion in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be collected sep-
arately from other waste. The separate collection of this equipment at the end of its lifetime is organized and managed by
the producer. The user will have to contact the manufacturer and follow the rules that he adopted for end-of-life equipment
collection. The collection of the equipment for recycling, treatment and environmentally compatible disposal, helps to pre-
vent possible adverse eects on the environment and health and promotes reuse and/or recycling of materials of the equip-
ment. Improper disposal of the product involves the application of administrative penalties as provided by the laws in force.
Equipment disposal
OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: +39 035.571.392
OPTIKA
Spain
OPTIKA
USA
OPTIKA
China
OPTIKA
India
OPTIKA
Central America
®
®
®
®
®
®
Modelli
Serie B-150 (B-151 / B-153 / B-155 / B-157 / B-159)
Serie B-150 ALC (B-151ALC / B-153ALC / B-155ALC / B-157ALC / B-159ALC)
Serie B-150R-PL (B-151R-PL / B-152R-PL / B-153R-PL / B-155R-PL / B-157R-PL / B-159R-PL)
Serie B-150
MANUALE DI ISTRUZIONI
Ver. 12.1 2022
This manual suits for next models
18
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