Optica XDS-3FL User manual

MICROSCOPES
I T A L Y
OPTIKA MICROSCOPES - ITALY
www.optikamicroscopes.com - [email protected]
Ver. 1.0.0
XDS-3FL
OPERATION MANUAL

1
Contents  XD S – 3 FL
Before use
1. Nomenclature…………………………………………………………………4
2. Assembl ing ……………………………………………………………………5
2-1. Assemble scheme …………………………………………………………5
2-2. Assemble steps …………………………………………………………………6
3. Operation ………………………………………………………………………………9
3-1. Illuminaton…………………………………………………………………………11
3-2. Filter preventing board ………………………………………………….………11
3-3. Centering bulb……………………………………………………………….…… 12
3-4. View field diaphragm………………………………………………………………13
3-5. Select fluorescence filter block………………………………………………….…13
4. General specification…………………………………………………………………14
5. Troubleshooting…………………………………………………………………….…15

Before use X D S – 3FL
Inverted fluorescence microscopesspecially suit for research work for activity cell, tissue, fluidness and sediment,
especially for biology, cytology, oncology, genetics and immunology etc. It is widely used in Lab, university,
medical, epidemic prevention and so on.
Figure 1
1. Notice:
ŁAs the microscope is a precision instrument, a lways
operate it with care, avoiding physical shake during the
operation.
łDo not let the microscope emerge in the sun directly, e
ither not in the high temperature, damp, dusty or acute
shake place. Make sure the worktable is horizontal.
Following environment is required: Indoor temperature:
5ć40 , Max relative humidity:80%.ć
ŃWhen moving the microscope, you should use both
hands to hold ķand ĸas shown on figure, and lay it
down carefully. (Figure 1)
Figure 2 ńFluorescence microscope should be used under dark
environment.
ŅIn order to protect eyes, do not stare at fluorescence light
directly.
ņThe bulb should be vertical under using, the inclined
angle should be less than 15 degrees, otherwise it will
make bulb damage.
ŇFluorescence sample will be faded by ultraviolet
radiation, so it can not for long save. Do not expose the
sample under fluorescence light for long time, it will be
quenched.
Figure 3 ňWhen working, the surface of the lamp holder Ĺand
bulb house ĺwill be very hot. Make sure there is
enough space (especially its above) for the heat
dissipating. (Fig. 1)
ʼnConnect the microscope to the ground to avoid lightning
strike.
ŊMake sure the switch Ļ,ļare at “!” position, cut off power supply and wait all parts cool down before
replace bulbs, fuse the fuse. It would be better to do so after cooling the bulb and the lamp holder. (Fig. 2, 3)
ƾStandard bulb:
Fluorescence light: 100W mercury bulb Transmitted light: 6V30W halogen bulb
ŋPower supply: 90-240V
ŌPlease use the plug provide our company.
2

XDS – 3FL
2
ˊ Maintenance
ŁWipe the lens gently with a soft lens tissue. Carefully wipe off oil or fingerprints on the lens surfaces with
tissue moistened with a little of 3:7 mixture of alcohol and ether or dimethy lbenzene.
ƾAlcohol and ether is flammable. Don’t place these chemical near to fire or fire source. For example,
when turning on or turning off the electric device, you should use these chemical in a ventilated place.
łDon’t use organic solution to wipe the surfaces of the other components. Please use the neutral detergent.
ŃIf the microscope damped by the liquid, you should cut off the power immediately and wipe it dry.
ńNever disassemble or service the microscope yourself. It will influence its function or damage it.
ŅAfter using, cover the microscope with a dust cover.
3. Safety Sign
Sign Signification
Hot at surface, do not touch.
Read manual before using. Faulty operation would lead to person hurt or instrument
trouble.
|Power swatch ON
OPower swatch OFF
3

1 . Nomenclature X D S - 3 FL
Ⓒܝᵓ:Filter plate
㤻ܝ㺙㕂䖲᥹ԧFluorescence device connector
∲♃♃⑤Light source of mercury bulb
XD30 ෎ᑻXD30 base
㤻ܝⒸܝ䬰Fluorescence filter
U-RFLT100 ⬉⑤ㆅU-RFLT100 Power box
Eye-protecting board connector for fluorescence
Light source of mercury bulb
XDSmicroscope stand Fluorescence filter blocks
U-RFLT100 Power supply
4

2. Assembling XDS – 3FL
2-1 Assembling scheme:
Numbers denote the assembling order.
ƾBefore assembling, make sure there is no dust or dirt. Assemble carefully and do not scrap any
part or touch the glass face.
ƾDuring assembling, please be aware of no scratching and no touching on lenses.
5
ĿľĻļĽķĸ
p
ower plug
p
ower supply for 100W fluorescence
b
ulb holde
r
mercury bulb
Light source with mercury bulb 100W
fluorescence hole cove
r
connector for fluorescence ĺĹEye-protecting board
Fluorescence filter blocks
right cove
r
ķother side
p
ole for fluorescence
Fluorescence filter blocks
left cove
r
p
ole for fluorescence
XDSmicroscope stand

Fluoecence
2-2 Assembling steps: ƾBefore assembling for fluorescence parts, please set
power supply at “OFF” and take off the plug in order
to operator’s safety.
Figure 4
2-2-1 Set fluorescence filter blocks
ŁLoose the lock-screw withķM4 inner hexagon spanner
and take off right cover (Fig. 4)ĸ
łMatch the dovetail glut of filter block B1 Ĺto the groove
of sliding channel ĺ, and push Ĺinto sliding channel
ĺat right.
ŃPut on the right cover ĸ, point the screw ķto the hole
Ļon sliding channel ĺand tight it with M4 spanner.
ńInsert the pole Ľinto the hole ļon right cover ĸ, and
point it to the hole at side of filter block B1Ĺ, then tight.
(Fig.5)
ŅLoose the screw cap Ŀa little, and turn the remark block
ľto make the letter “B” point to operator, then tight Ŀ.
ƾXD S-3FL fluorescence microscope can only be equipped
with 2pcs filter blocks. The standard is B1 and G1.
Others for selection.
Figure 5
Figure 6 ƾUse the same way to set filter block G1 to the left.
ƾPlease be aware the remark (such as: DMB1) on the side
should be correctly, do not overturn with up and down.
ƾDuring observation, both right and left cover can’t be
taken off because it protects the filter while filter
exchanging.
2-2-2 Set the fluorescence connector
ŁHook the hole ķto take off the fluorescence hole cover
ĸ. (Fig. 6)
łInsert the fluorescence connector to the fluorescence holeĹto make the screw point to thread , and tight 3 screwsĺĻwith M4 spanner. (Fig.7)
ŃTurn off one of handles frĽom fluorescence preventing
board ľand insert the board ľinto connector, you will
find sound to mean it at middle position (there are 3
positions). And tight the handle Ľ. (Fig. 8)
6
Figure 7

XDS – 3FL
Figure 8
2-2-3 Set light source with mercury bulb
ŁLoose screw ķwith M4 inner hexagon spanner. (Fig.9)
łInsert the light source with mercury bulb ĸinto
fluorescence connector Ĺ, and rotate ĸto make its upper
plane ĺas horizontal, then tight the screw ķwith M4
spanner.
ƾDuring observation, please make sure enough space for
heat-reek around the bulb source, especially the upper
and bottom of bulb.
ƾDuring observation, please make sure enough distance
between light source and power plug to avoid power
plug melted by heat on light source.
Figure 9
2-2-4 Set mercury bulb
ŁLoose the lock-screw completely bķy M4 inner hexagon
spanner, rotate about 45 degrees as the direction showed,
and take off the bulb holder ĸ. (Fig. 10)
łLoose the lock screw Ĺfor mercury bulb and take off the
supporting pole ĺ. Insert the anode side (big side) on
mercury bulb into the anode holder ļand set negative
holder on small side of bulb, then tight the screw Ĺ. (Fig.
11)
Figure 10 ƾPlease make sure the mercury bulb put vertically. If
there is aspirating hole on bulb, please make sure the
hole directly to ceramic holder.
ŃPut the bulb holder ĸinto its house and tight the screw
ķ.
ƾIt is the same way to replace bulb.
ƾReplace bulb during or after operation:
During or just after operation, the bulb, bulb house
and around is very hot. Before replace the bulb, please
set the power supply for fluorescence at “o”(OFF) and
take off the power plug. After all cooling down to
replace bulb.
ƾAfter replace bulb, please be aware the timer on power
s upply at zero. See details “3-1”
Fi
g
ure 11
7

XDS – 3FL
2-2-5 Connect power supply
ŁMake sure that the main power supply and fluorescence
power supply at “o” (OFF).
łConnect one side the plug ķto the connector ĸon light
source with mercury bulb and lock the screo. (Fig. 12)
ŃUse same way to connect other side of the plug ķto
connector fluoĹrescence power supply.
ńConnect one side of the plug ĺto socket Ļon
fluorescence power supply and other side to power supply
socket.
ƾThe fluorescence power supply is 110-240V.
ƾPlease curve and enlace the plug wire softly to avoid
damage it.
ƾPlease use the standard plug wire provide our
company. Select suitable one when missing or
damagment.
ƾConnect the power supply correctly, be sure the
instrument earth-connecting.
2-2-6 Replace fuse
Before replace fuse, please set the main power supply and f
luorescence power supply at “o” (OFF) and take off plug.
Use “_” screw driver to take off fuse set ķfrom holder ĸ
and change a new one. (Fig. 13)
ƾFuse specification: 250V, 3.15A
2-2-7 Set eye-protecting board
Figure 12
Figure 13
Figure 14 ŁLoose screw ķtill enough to put in trinocular cover.
(Fig.14)
8
łSet the holder ĸclose with trinocular cover Ĺand
horizontal the eye-protecting board, then tight the screw
ķ.
ŃLoose the screw ĺa little to let connecting pole Ļ
moving as showed direction. And adjust the
eye-protecting board to suitable position, then tight the
screw ĺ.
Figure 15

3. Operation XDS – 3FL
centring screw for view field diaphragm
handle on filter preventing board
filter
p
reventin
g
boar
d
adjusting pole for view field diaphragm
fluorescence filter blocks
!fluorescence filter block B1
!fluorescence filter block G1
filter exchanging pole
!filter exchanging pole B1
!filter exchanging pole G1
9

XDS – 3FL
10
focusing lock-knob
horizontal adjusting screw
for mercury bulb
vertical adjusting screw for mercury bulb
focusing screw for reflecting lens
horizontal centering screw for
reflecting mirro
r
vertical centering screw
for reflecting lens
ammeter
voltmeter
power switch
timer
indicator adjusting knob for electric current
power socket
power connector
Fuse
timer reset

XDS – 3FL
While using transmitted illumination, the operation way is the same as inverted biological microscope. Below is
the operation way under reflected fluorescence illumination.
3-1 Illumination
ŁAfter connect with main power supply, please set the
switch ķon fluorescence power supply at “_” (ON),
then the indicator light. It costs 5 minutes to warm up
mercury bulb. (Fig. 16)
łUse adjusting knob for electric current ļto make
out-put power as same as mercury bulb 100W.
Min. scale for ammeter isĻ0.4A and Max. is 2A.
Min. scale for voltmeter is 0.4AĽand Max. is 2A.
ƾNo need to switch on for transmitted illumination
when using fluorescence illumination.
Figure 16 ƾWhen use mercury bulb for first time or just replace
bulb, please set the timer reset Ĺto highest position
as till timer ĺindicates “000.00”, then set Ĺto
lowest position.
ƾDo not cut off power supply within 15 minutes after
mercury bulb light on to avoid it damaged.
ƾIn order to prolong the life of mercury bulb, please do
not re-light on it within 3 minutes after it turned off.
ƾWhen the timer ĺindicates “200.00”, it means the
mercury bulb had lighted on for 200 hours and it is its
utmost and it is time for replacement.
ƾDon’t stare at fluorescence light directly.
11
3-2 Use filter preventing board
Pull the filter preventing board to tķhe most left position,
it prevents fluorescence light while middle position for light.
If set¶32 filter in the right groove on board ķand pull it to
most right position for light filtration.
ƾWhen no need observation under fluorescence light,
please pull the filter preventing board to most left
position to protect the slide from fluorescence quenching
by long time exposure.
Fi
g
ure 17

XDS – 3FL
3-3 Centering mercury bulb
ŁTurn the nosepiece ķto make blank hole into light-path.
T take off nosepiece cap when it has. (Fig.18)
Figure 18 łPull filter exchanging pole ĸto the middle position for
B1 observation.
ŃTurn adjusting pole forĹview field diaphragm by
clock-wise to make the view field to largest. (Fig. 18)
ńSet a white paper on stage and pull filter preventing board
ĺto middle position for observation.
ŅAdjust focusing lock-knob ,Ļvertical adjusting screw Ľ
for mercury bulb, horizontal adjusting screw ļfor
mercury bulb to make the bulb image on white paper.
(Fig.18, 19, 20a)
ņAdjust focusing screw for reflecting lens,Ŀhorizontal
centering screw for reflecting mirror, vertical centeringľscrew for rŀeflecting lens to make the bulb reflected
image on white paper. (Fig. 19, 20b)
ŇAdjust screw ľand ŀto make the bulb image and
bulb reflected imagte symmetry. Adjust screw Ŀto make
both images with same size. (Fig. 19, 20c)
Figure 19
12
ňAdjust screw ļto make the two images superposition.
(Fig. 19, 20a)
ʼnTurn 10X objective into light-path and set slide on stage
for observation under B1. Find the image and make it
clear.
ŊObserve through eyepieces and adjust focusing lock-knob
Ļto make view field to best, then lock the knob.
ƾCentering the bulb after it warmed up will be more
precision.
ƾAdjust the vertical and horizontal screw for bulb
image, the reflected image will also be moved.
ƾAfter replace the mercury bulb, it should be re-center
the bulb.
Bulb image
Reflected image
Fi
g
ure 20

XDS – 3FL
3-4 View field diaphragm
Field diaphragm limits the light beam diameter into
condenser, therefore eliminates the surround light in order to
enhance image contrast. When field diaphragm image is
just at edge of field, objective can provide best performance
and the image is clearest.
ŁTurn adjusting pole ķfor view field diaphragm
clock-wisely to open the view field diaphragm, otherwise to
close. (Fig. 21)
łObserve through eyepiece to find image of view field
diaphragm.
ŃAdjust two screws ĸat both side of view field diaphragm
to center the image.
ńOpen the field diaphragm gradually. If the image of field
diaphragm is just inscribed to the view field, it means the field
diaphragm had been centered. (Fig.22)
ŅWhile actual operation, please open the field diaphragm a
little to make it ex-scribed with view field in order to obtain
better image.
ƾIn order to prevent the specimen from fluorescence
quenching, don’t expose the same position of the
specimen for long time.
3-5 Fluorescence filter block
The filter block should be accordance with specimen. Please
see the parameters “Sheet 1” for spectrum.
Figure 21
Figure 22
Fi
g
ure 23
Standard filter block: B1, G1
ŁPull the filter preventing board ĸto the most right position. (Fig.3)
łUse filter exchanging pole ķto select needed filter block.
ƾWhen using transmitted illumination, please pull both B1, G1 filter blocks to most left and right
position.
Filter block Module Specification Fluorescence dyes
Exciter 475AF40
Dichroic 505DRLP
B1
Emitter 535AF45
EGFP, FITC, Cy2@,
AlexaFluor@488,
DIO, Fluo-4
Exciter 560AF55
Dichroic 595DRLP
G1
13
Emitter 645AF75
Texas Red@, Texas
Red@-X, Cy3.5@,
Mito Tracker@ Red

4. Specification XDS – 3FL
Optical system Color Corrected Infinity Optical System
Head trinocular,45° inclined
inter-pupillary distance: 50-76 mm
PL10X/22mm high point plan eyepiece
Eyepiece
Nosepiece Build-in quintuple nosepiece
Infinity long working distance plan achromatic objective 4X,10X,20X,40X,60X
Objective Infinity long working distance plan phrase contrast objective 10X,20X,40X
Fine and coarse coaxial, torque adjustment for coarse
Focusing
system
Precimen: coarse-38mm, fine-0.2mm, Min.-0.002mm
Moving range: up 6.5mm and down 2.5mm from the focus position(1.5mm above stage)
160*250mm plan stage, can be equipped with extending board and mechanical stage
Stage Extending board: 70*80mm
Mechanical
stage Moving range: 120*80mm for low adjustment, with sample holders
Transmitted
illumination
90-240V power supply with 6V30W halogen, pre-centered, adjustment for brightness
Super long working distance condenser NA0.3, WD72mm
Reflected
fluorescence Equipped with B,G filter blocks, with centering view field diaphragm and sliding filter
preventing board
illumination
Mercury bulb
house Centering mercury bulb 100W, with adjusting reflected lens
Fluorescence
power supply
100-240V input AC power supply
House size: 235*180*111mm
"Indoor
ƽAltitude: 2000m Max.
ƽTemperture: 5ćˉ40ć(41°Fˉ104°F)
Operation ƽHumidity: 80% for 31ć(88°F)
Enviroment 70% for 34 (93°F)ć
60% for 37 (99°F)ć
50% for 40 (104°F)ć
ƽPollution: 2 (Refer to IEC664)
14

15
5. Troubleshooting XDS – 3FL
Symptom Cause Remedy Refer to
Page.
1. Opticis
View field diaphragm is not large
enough Open the diaphragm more 13
Filter blocks are not at correct
position Adjust them 12
ŁThe lamp is not
bright enough
Filter preventing board prevented
light Adjust filter preventing board 11
The objective is not under
light-path
Turn the nosepiece to lock
position ˉ
Dirt on lens Clean it ˉ
Field diaphragm is too large or
narrow Adjust it 13
łImage blur
The filter block is not accordance
with specimen Adjust filter block 13
Nosepiece is not at lock position Turn the nosepiece to lock
position ˉ
Filter blocks are not at correct
position Adjust them 12
Bulb is not centered Center the bulb 12
Condenser adjusting knob is not at
correct position Adjust it 12
Filter preventing board prevented
light Adjust filter preventing board 11
Ńblur field or
brightness
asymmetry
Field diaphragm is not centered Adjust it 13

16
XDS – 3FL
Symptom Cause Remedy Refer to
Page.
2. Electrics
No power supply Check the connection of power
supply 8
ŁIndicator on
fluorescence power
supply is not light The fuse is burnt out Replace fuse 8
Incorrect connection of power
supply Check the connection 8
destruction of power plug Change a new one 8
łIndicator on
fluorescence power
supply light, but bulb
is not light The mercury bulb burnt out Replace bulb 7
The power supply has just been
connected Wait the bulb warm up 11
Incorrect connection of power
supply Check the connection 8
ŃMercury bulb
flashes
The mercury bulb will be burnt
out soon Replace bulb 7

Page 8
RECYCLING AND RECOVERY
The appliance reports the symbol:
This symbol means that the appliance can be a precious source of raw materials. Therefore, it must
not be disposed of as waste, but separately collected for the recycling and the recovery of the mate-
rials it contained in it. Such materials, if improperly dispersed into the environment, can be harmful
to the environment and to human health.
The producer of the equipment, Optika Microscopes, recovers, re-uses and recycles the raw mate-
rials contained in the equipment. Such recovery, however, needs your help.
When, at the end of its operating life, you shall decide to dispose of the apparatus, do not try to open
it, nor to use parts of it in ways other than reported in this Manual, but bring it back to the Distributor
you bought it from, or to other Optika Microscopes distributors. The Distributor shall collect the ap-
paratus free of charge.
The recovery of the raw materials shall then take place in accordance with the European Directive
2002 / 96 / EC and all other relevant Directives. Never disassemble, nor dispose of as waste, appa-
ratus reporting the “crossed bin” symbol indicated above.



OPTIKA MICROSCOPES - ITALY
www.optikamicroscopes.com - [email protected]
www.optikamicroscopes.com [email protected]
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