Optica B-150V Series User manual

Model
B-151R-PLV
B-152R-PLV
B-159R-PLV
B-151V
B-153V
B-150V Series
INSTRUCTION MANUAL
Ver. 1.1 2022

Page 2
1. Warning 3
2. Safety Information 3
3. Package content 4
3.1 B-151V / B-151R-PLV 4
3.2 B-152R-PLV / B-153V 4
3.3 B-159R-PLV 5
4. Unpacking 6
5. Intended use 6
6. Symbols and conventions 6
7. Instrument description 7
7.1 B-151V / B-151R-PLV 7
7.2 B-152R-PLV / B-153V / B-159R-PLV 8
8. Assembling 10
8.1 Assembling the microscope with observation head 10
8.2 Assembling the microscope with digital head 10
8.3 Polarizing set (optional) 11
9. Use of the microscope 12
9.1 Light intensity adjustment 12
9.2 Coarse focus tension adjustment 12
9.3 Stage 12
9.4 Useofdi󰀨usinglter 12
9.5 Use of oil immersion objective 13
9.6 Aperture diaphragm 13
9.7 Use with rechargeable batteries 14
9.8 Use of the polarizer (optional) 14
10. Use of the digital head 15
10.1 Operation procedure 15
10.1.1 Connect the power adapter to the camera 15
10.1.2 Use of the rear buttons 15
10.1.3 Snap 15
10.2 Menu and Functions 16
10.3 Operating Instructions 17
10.3.1 White Balance 17
10.3.2 Exposure 17
10.3.3 Line Measurement 18
a) Cross Line 18
b) Calibration 18
c) Measurement 19
10.3.4 Color Adjustment 19
10.3.5 Record Video 19
10.3.6 Other Functions 20
a) Monochrome 20
b) Flip 20
c) Field of View (FOV) 20
10.3.7 Other Settings 20
a) Power Frequency 20
b) SD Management 21
c) Timed Snap 21
d) Language 21
e) Factory Reset 22
f) Software Upgrade 22
f.1) System normally upgraded 22
f.2) System upgrade failed 22
g) Version 23
10.4 Failure analysis and troubleshooting 23
11. Maintenance 24
12. Troubleshooting 25
Equipment disposal 26
Table of contents

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4
3. Package content
3.1 B-151V / B-151R-PLV
3.2 B-152R-PLV / B-153V
① Frame
② Monocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupplyformicroscope
⑨ Camera+display
⑩ Powersupplyfordisplay
⑪ Diusinglter
⑫ SDcard+SDcardreader
③
④
⑤
⑥⑦
⑧
①
②
① Frame
② Monocularobservationhead
③ Eyepiece
④ Tensionadjustmenttool
⑤ Objectives
• B-152(4X/10X/40X)
• B-153(4X/10X/40X/60X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
⑨ Camera+display
⑩ Powersupplyfordisplay
⑪ Diusinglter
⑫ SDcard+SDcardreader
③
④
⑤
⑥
⑦
⑧
①
②
⑨
⑩⑫
⑨
⑪
⑫
⑨
⑪
⑩
⑩

Page 5
3.3 B-159R-PLV
① Frame
② Binocularobservationhead
③ Eyepieces
④ Tensionadjustmenttool
⑤ Objectives(4X/10X/40X/100X)
⑥ Dustcover
⑦ Greenlter
⑧ Powersupply
⑨ Immersionoil
⑩ Camera+display
⑪ Powersupplyfordisplay
⑫ Diusinglter
⑬ SDcard+SDcardreader
③
④
⑤
⑥⑦
⑧
①
②
⑨
⑩
⑫
⑪
⑬

Page 6
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 7
7. Instrument description
7.1 B-151V / B-151R-PLV
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
CAMERA
DISPLAY
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
(ONLY “R”
VERSION)
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
STAGE CLIPS

Page 8
X/Y MOVEMENT
KNOBS
7.2 B-152R-PLV / B-153V / B-159R-PLV
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
CAMERA
DISPLAY
MAIN SWITCH / INTENSITY
ADJUSTMENT DIAL
CONDENSER
STAGE
LED CHARGE
INDICATOR
(ONLY “R”
VERSION)
NOSEPIECE
OBJECTIVES
TENSION
ADJUSTMENT
COLLAR
SLIDE HOLDER

Page 9
SD CARD SLOT
SNAP BUTTON
ON/OFF BUTTON
POWER SUPPLY
CONNECTOR
MENU CONTROL
BUTTONS

Page 10
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 3ig. 3
8. Assembling
8.1 Assembling the microscope with observation head
1. Remove the dust cap from the microscope frame and from
the bottom of the observation head.
2. Insert the optical head above the stand and tighten the screw
①. (Fig. 1)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
3. Insert the eyepiece into the empty tube of the optical head.
(Fig. 2)
4. Insert the microscope power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)
8.2 Assembling the microscope with digital head
1. Remove the dust cap from the microscope frame and from
the bottom of the digital head.
2. Insert the digital head above the stand and tighten the screw
①. (Fig. 4)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
FFig. 4ig. 4
①
①

Page 11
8.3 Polarizing set (optional)
1. Place the polarizer on the light exit ① at the base of the mi-
croscope. (Fig. 5)
2. Loosen the head xing knob ② and remove the head or the
digital head from the microscope frame. (Fig. 6)
FFig. 5ig. 5
FFig. 6ig. 6
①
②
3. Insert the analyzer ③ into the hole inside the frame. (Fig. 7)
4. Put back the head into its original position and lock the xing
knob.
FFig. 7ig. 7
③

Page 12
①
FFig. 8ig. 8
FFig. 9ig. 9
FFig. 10ig. 10
③
9. Use of the microscope
9.1 Light intensity adjustment
Operate on the light intensity adjustment dial ① to turn ON /
OFF the microscope and to increase / decrease the illumination
voltage. (Fig. 8)
9.2 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-set in the factory.
1. To modify the tension according to personal’s needs, rotate
the ring ② using the provided tool (Fig. 9).
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension.
9.3 Stage
All models except B-151 series
Stage accepts standard slides 26 x 76 mm, thickness 1,2 mm
with coverslide 0,17mm. (Fig. 10)
1. Open the spring arm of the slide holder ③ and place the slide
from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the fall-
ing of the slide.
②
9.4 Useofdi󰀨usinglter
All models with Digital head
In order to ensure an even illumination with low magnication
objectives, it is strongly suggested to use a di󰀨using lter to be
placed in the lter holder placed under the condenser.
1. Rotate the lter holder ④ placed below the condenser.
2. Insert the di󰀨using lter ⑤ in its place. (Fig. 11)
3. Put the lter holder into the light path.
FFig. 11ig. 11
④
⑤

Page 13
9.5 Use of oil immersion objective
B-159R-PLV only
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 12)
• Make sure that there are no oil bubbles. Air bubbles in
the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully open the ap-
erture diaphragm and observe the objective exit pupil. (The
pupil must be circular and bright).
• To remove the bubbles, gently move the nosepiece to the
right and left to move the immersion objective a few times
and allow the air bubbles to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and obtain an
optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper towel or a
lightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute ethyl alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the specimen would
bedi󰀩cult(evennotimpossible)duetothepresenceof
an additional thickness on the objective.
9.6 Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aperture dia-
phragm a󰀨ects the image contrast. Increasing or reducing
this value one can vary resolution, contrast and depth of
focus of the image. Move the diaphragm lever ① (Fig. 13)
toward left or right to decrease or increase the N.A. value.
• With low contrast specimens set the numerical aperture to
about 70%-80% of the objective’s N.A. If necessary, remove
on eyepiece and, looking into empty sleeve, adjust the con-
denser’s diaphragm in order to obtain an image like the one
in Fig. 14. FFig. 13ig. 13
①
FFig. 14ig. 14
APERTURE
DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
FFig. 12ig. 12

Page 14
FFig. 15ig. 15
②
9.7 Use with rechargeable batteries
This operation can be done only with “R” models.
When the microscope is plugged with the power supply, the LED
indicator for the battery recharge ② is lit. (Fig. 15)
• Power supply connected, battery low: LED is RED. When
charging is completed, it turns GREEN.
• Power supply not connected, microscope o󰀨: LED is always
OFF.
• Power supply not connected, microscope on, battery
charged: LED is GREEN.
• Power supply not connected, microscope on, battery almost
low: LED is RED.
9.8 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer until the
darkest position is achieved.
3. Once the dark is achieved (“extinction” or “Crossed Nicol”
position) it is possible to begin the observation.

Page 15
10. Use of the digital head
10.1 Operation procedure
10.1.1 Connect the power adapter to the camera
1. Insert the power adapter into the power socket ① placed on
the back side of the camera. (Fig. 16)
• After the connection, the red light will light up.
FFig. 16ig. 16
①
FFig. 18ig. 18
10.1.2 Use of the rear buttons
1. Use the rear buttons to operate the camera functions and
adjust the camera parameters.
2. After modifying the parameters, a dialog box appears allow-
ing to save the modied parameters.
3. In the upper left corner of the screen, “Param. saved “ ap-
pears. (Fig. 18)
FFig. 19ig. 19
10.1.3 Snap
• The snap button ③ is on the right side of the camera, above
the power button. (Fig. 17)
1. Press it to capture the current image on the screen and store
it in the SD card.
2. The screen shows “Snap Succeeded”, which means that the
picture has been taken successfully. (Fig. 19)
FFig. 17ig. 17
②
2. Press the power button ②. (Fig. 17)
3. The indicator light will change from red to green, and the
camera will start up.
• Warning: disconnect the power supply if the equipment
is not used for a long time.
③

Page 16
10.2 Menu and Functions
1. Press the MENU button to call out the menu.
2. The position of the current cursor (that is, the position of the
highlighted icon) is the white balance function option. (Fig.
20)
3. Press ↑↓ for function selection, press → to enter the sub-
menu interface of the corresponding functions, press MENU
to hide the interface, and save all parameters that have been
modied.
FFig. 20ig. 20
4. The specic functions of this menu are shown in Fig. 21.
Fig. 21

Page 17
10.3 Operating Instructions
10.3.1 White Balance
After entering the White Balance menu, the default option is
“Auto White Balance”. (Fig. 22)
FFig. 22ig. 22
FFig. 23ig. 23
When the e󰀨ect of automatic white balance is not ideal due to the
di󰀨erence of color temperature between di󰀨erent light sources,
manual white balance can be used to adjust the parameters of
color temperature, R, G and B respectively. (Fig. 23)
10.3.2 Exposure
After entering the exposure menu, the default option is “Auto ex-
posure”. (Fig. 24)
• Under automatic exposure, you can adjust the target “value”
to adjust the degree of exposure.
FFig. 24ig. 24
FFig. 25ig. 25
• In manual exposure, you can also adjust the exposure by
adjusting the value of “exposure time“. (Fig. 25)

Page 18
10.3.3 Line Measurement
This menu includes Cross Line, Calibration and Measurement.
(Fig. 26)
FFig. 26ig. 26
FFig. 27ig. 27
FFig. 28ig. 28
FFig. 29ig. 29
a) Cross Line
• Four groups of cross lines are provided in red, blue, green
and white colors. You can choose according to your require-
ments.
1. Enter the Cross Line menu. (Fig. 27)
• “ID” refers to the number of each group of cross lines.
• “Display” informs whether the lines are displayed.
• “X Position” and ”Y Position” adjust the position of the center
point of the lines.
You can select and press the “Turn o󰀨 All Cross lines” to hide all
cross lines.
b) Calibration
• Default calibration values are available for this system. How-
ever, due to the di󰀨erent objective standards of the micro-
scope, the calibration value may have some discrepancies,
so it is advisable to perform a new calibration.
• Calibration procedure requires a calibration micrometer.
The calibration procedure is described below.
1. Put the micrometer on the stage and adjust the microscope
so that the micrometer scale is clearly displayed on the
screen.
2. In order to facilitate calibration, it is suggested to rotate the
camera so that the micrometer is placed horizontally in the
screen without being hidden by the menu.
3. After entering the Calibration menu, the dialogue box in Fig.
28 appears.
4. Adjust the positions of the calibration start and end points to
make the calibration line coincide with the micrometer scale
and try to select the maximum length that can be displayed,
in order to make the most accurate measurement. (Fig. 28)
5. The minimum range of the selected micrometer is 0.01 mm
(10 micron). Fig.29 shows the image under a 10x objective
lens. At this time, the “magnication” is set to “10X”, the “unit”
is marked as “μm”, and the “length” is set to “40”.
6. Repeat the same procedure for all objective available on the
microscope.
• After adjusting the parameters, exit the calibration interface:
the calibration is completed.

Page 19
c) Measurement
• The system needs to be calibrated before measurement can
be performed.
1. Enter the Measurement menu.
2. Select the measurement magnication.
3. Move the starting and ending point of X and Y position by
pressing ← and →: the measurement length is displayed in
real time. (Fig. 30)
• Changes in the percentage of the eld of view do not a󰀨ect
measurements.
FFig. 30ig. 30
FFig. 31ig. 31
FFig. 32ig. 32
10.3.4 Color Adjustment
1. After entering the color adjustment menu (Fig. 31), Satura-
tion, Hue, Brightness and Contrast can be adjusted to make
the picture reach the required level.
2. In order to facilitate the color adjustment, the “Color Value
Reset” option is available in the menu.
• When selected and pressed, all the color values in the menu
will be restored to the default value.
10.3.5 Record Video
1. Press M button to show menu in the screen (Fig. 32) and
choose Record Video option by pressing ↑ or ↓.
2. Press → button to start recording. The recording time is
shown in the right part of the screen beside the dialog.
3. If you want to quit recording, just press M button.
• Before recording the video, you should check whether
toinsert anSD cardwith a FAT32 le system andfree
space. You cannot take a photo during the recording
process.

Page 20
10.3.6 Other Functions
The menu includes functions of Monochrome, Flip vertical, Flip
horizontal and FOV. (Fig. 33)
1. The numerical option can be adjusted by ← and →, and the
switch options can be opened and closed by →.
2. After the adjustment is completed and the main menu is
closed, the settings will be saved.
3. The function settings will be retained when the next boot is
started.
a) Monochrome
The function produces visual images in varying tones of a single
color (gray).
b) Flip
This function is divided into vertical ipping and horizontal ip-
ping.
c) Field of View (FOV)
This function can adjust the range of observation eld.
1. Use ← and → in the menu to adjust the size.
2. When the menu is not displayed, press ↑ and ↓ to adjust: the
current percentage value appears in the upper left corner.
FFig. 33ig. 33
FFig. 34ig. 34
10.3.7 Other Settings
This menu contains Power Frequency, SD Management, Timed
Snap, Language, factory Reset, Software Upgrade, Version.
(Fig. 34)
a) Power Frequency
CMOS sensors have a rolling curtain e󰀨ect that causes icker
problems, which can be solved by capturing a line of pixels as an
integer (n) times the icker period. Among them, 60Hz in North
America and 50Hz in Europe. (Fig. 35)
• DC (DC): for DC (DC) light source, there is no light uctu-
ation, so there is no need to compensate the ashing light
source.
• AC (50Hz): select AC (50Hz) to eliminate the dark strip of the
lamp curtain caused by the 50Hz light source.
• AC (60Hz): select AC (60Hz) to eliminate the dark strip of the
lamp curtain caused by 60Hz light source.
FFig. 35ig. 35