PEAK INSTRUMENTS C-7000 Series User manual

C-7000 Series User Manual PEAK INSTRUMENTS INC.
C-7000 Series Spectrophotometer
Operating Manual and Maintenance Guide
PEAK INSTRUMENTS INC.
Version 1701

C-7000 Series User Manual PEAK INSTRUMENTS INC.
CONTENTS
I. Spectrophotometer Overview ..............................................................................................................................1
1. Basic Operating Principle ..........................................................................................................................................1
2. Performance Indicator Definitions ...........................................................................................................................3
3. Application ...............................................................................................................................................................4
4. Using conditions .......................................................................................................................................................4
II. Product Features and Installation ......................................................................................................................5
1. Features ....................................................................................................................................................................5
2. Performance Indicators ............................................................................................................................................6
3. Main Functions .........................................................................................................................................................7
4. Structure Introduction ..............................................................................................................................................8
5. Instrument Installation ……………………………………………………………….………………………………………………………………………10
III. Key Definitions and Basic Operation ..................................................................................................................11
1. Button panel schematic diagram ..............................................................................................................................11
2. Button function description ......................................................................................................................................11
3. Basic operation ..........................................................................................................................................................12
IV. Instrument Self-test ..............................................................................................................................................12
1. Communication port inspection……………………………………………………..…………………………………………………………………….12
2. File system check .......................................................................................................................................................12
3. Filter positioning ........................................................................................................................................................12
4. Light positioning ........................................................................................................................................................12
5. Printer Check .............................................................................................................................................................13
6. Tungsten lamp examination ......................................................................................................................................13
7. Deuterium lamp examination ....................................................................................................................................13
8. Signal detector check .................................................................................................................................................13
9. Wavelength Calibration .............................................................................................................................................13
10. Dark current correction ...........................................................................................................................................13
11. System parameters check ........................................................................................................................................13
V. Photometric Measurement ....................................................................................................................................14
1. Function description ...................................................................................................................................................14
2. Set the measurement mode .......................................................................................................................................14
3. Set wavelength ...........................................................................................................................................................15
4. Correction 100%T/0Abs ..............................................................................................................................................15
5. Measurement data .....................................................................................................................................................15
6. Delete data .................................................................................................................................................................16
7. Save the file ................................................................................................................................................................16
8. Open the file …………………………………………………………………………………………………………………………………………………………17
VI. Quantitative measurement ..................................................................................................................................17
1. Set parameter .............................................................................................................................................................18
2. Standard sample measurement ..................................................................................................................................18
3. Sample measurement ..................................................................................................................................................19
4. Open standard curve ....................................................................................................................................................19

C-7000 Series User Manual PEAK INSTRUMENTS INC.
VII. Time Scan (Kinetics)……………………………………………………………………………………………………………….………………………..…20
1. Function description ..............................................................................................................................................20
2. Time scanning parameters set ...............................................................................................................................20
3. Data test .................................................................................................................................................................21
4. Data list ..................................................................................................................................................................21
5. Slope calculation.....................................................................................................................................................22
VIII. Multi-wavelength Test ........................................................................................................................................22
1. Function description ...............................................................................................................................................22
2. Set parameter .........................................................................................................................................................23
3. Correction 100%T/0Abs ..........................................................................................................................................23
4. Data test .................................................................................................................................................................23
IX. System Setup ….....................................................................................................................................................24
1. Dark current measurements....................................................................................................................................25
2. Looking for deuterium lamp curve ..........................................................................................................................25
3. Time and date settings ............................................................................................................................................26
4. Light source management .......................................................................................................................................26
5. General ....................................................................................................................................................................27
6. File System ...............................................................................................................................................................27
7. USB storage device ..................................................................................................................................................28
8. Restore factory settings ...........................................................................................................................................28
9. System information .................................................................................................................................................29
X. Instrument Care and Maintenance .........................................................................................................................29
1. Routine precautions ................................................................................................................................................29
2. Instrument Care and Maintenance .........................................................................................................................30
XI. Instrument Troubleshooting ..................................................................................................................................30
1. Power failure ...........................................................................................................................................................30
2. Self-test failure ........................................................................................................................................................31
3. Other failure ............................................................................................................................................................31
XII. Light source replacement .....................................................................................................................................32
1. Tungsten lamp replacement ....................................................................................................................................32
2. Deuterium lamp replacement .................................................................................................................................33

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I. Spectrophotometer Overview
1. Basic Operating Principle
1) The nature of absorption: spectrophotometric analysis method is the use of substances to choose a different
wavelength of light absorption characteristics established. Typically using a prism or grating to obtain
monochromatic light that passes through the continuous monochromatic solution, the solution was measured and
the absorption of each wavelength, the absorption spectrum curve obtained.
Absorption spectrum selective absorption of light from the material, which is the material of macroscopic phenomena,
and the nature of the molecular absorption is the result of internal movement and light interaction. When molecules
absorb certain wavelengths of spectral energy or by some wavelengths of the spectrum is absorbed to form the
absorption spectra. The smaller the energy absorption, the wavelength of light corresponding to the absorption peak at a
longer wavelength. When the infrared absorbent is formed in the infrared absorption spectrum, if the energy absorption
is larger, the shorter the wavelength corresponding to the absorption peak at a shorter wavelength, when generating the
ultraviolet absorption spectra of absorption in the ultraviolet region.
2) Absorption Law - Lambert Beer law: when a parallel beam through the homogeneous solution, the absorbance of
monochromatic light is proportional to the product of the solution concentration and thickness.
Its digital expression: A=KCL=LogI0/I=-LogT
Premise absorpon laws established numeric expression: ① the incident light is monochromac ② the
absorption process without interacon of each substance, the absorbance of each substance has addivity ③ the
role of light and matter is limited to the absorption process, no fluorescent and photochemical scattering
phenomena ④ absorbent system is a connuous uniform distribution
3) Impact spectrophotometry reasons:
A. Non-absorption errors caused by radiation and matter
B. Fluorescence and photochemical reactions, in general, errors fluorescence spectrophotometry produced
negligible fluorescence efficiency is very small in most cases the color system, and the fluorescence emission is
isotropic, only a small portion along the transmitted light direction into the detector, the measurement of
absorbance is low, resulting in a negative deviation. Depends on the instrument to measure the impact on the
absorption of fluorescence great extent on the optical absorption cell and detector design.
C. Reflection and scattering, absorption law applies only to homogeneous medium absorption system, turbid solution so
that the measured increase in absorbance due to scattering, resulting in deviation from Beer's law
D. Non-ideal instrument error caused
E. Beer's law deviation polychromatic contrast, the majority of the photometer can only get close to monochromatic light
with a narrow lumen, in fact, there is still a polychromatic nature, can lead to deviations from Beer's law. Deviation
depends on the two monochromatic molar absorptivity difference △ ε, | △ ε | is very small, can be approximated that

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monochromatic, at low concentrations, curve remains linear, but larger concentrations, with concentration increases, AC
curve bend more serious, there is Beer's law applies only to dilute solutions
F. Stray light, stray light entering the detector means is unnecessary components to be tested at other wavelengths
outside the range of the wavelength spectral bandwidth. The main dispersive element from a prism or grating
spectrometer, a mirror, a lens surface scattering, dust and other inner walls of monochromator components and
diffuse reflection and other scars, the stray light can cause serious measurement error. The instrument is the
smallest wavelength of energy, usually at a maximum stray light (such as deuterium lamp 220nm, tungsten lamp
340nm)
G. Slit width, the slit width of the spectrum affects not only the purity, but also affect the absorbance. When
quantitative analysis in order to obtain sufficient measuring signal, the slit should be larger, in the qualitative
analysis of the use of a smaller slit when the entrance slit and the exit slit width equal to the width of the slit caused
minimum error
H. Wavelength scale ruler of error, the wavelength of the gauge that wavelength accuracy of the instrument, such
as a large error or correction, the spectral measurements produce errors that affect the accuracy of absorbance
measurements (in the absorption spectrum of the peak of the more significant )
I. Impact of non-parallel incident, one of the prerequisites ear than the law is the use of a parallel incident beam to
ensure that all beams through the same thickness of the absorbing medium, when a large deviation from
parallelism when the incident beam, obviously lead to deviations from Beer's law. If the instrument is in moderate-
intensity beam deviation from parallelism, absorbance measurement error is generally caused by less than 0.5%
J. Photometric scale error, photometric accuracy of the scale that is transmittance, which directly affects the
accuracy of the magnitude of the error photometric measurements.

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2. Performance Indicators Definitions
1) Optical system: usually refers to the formal structure of the optical system, at present, domestic and international
institutions often used as a photometer industry type and CT auto collimation two structures;
2) Wavelength range: means for wavelength photometer can difference between maximum and minimum values of
the test;
3) Wavelength accuracy means the actual wavelength difference between the real wavelength and the setting
wavelength. Each wavelength photometer to be in a lot of points to check wavelength accuracy; spectrophotometer
wavelength accuracy is an important technical indicators, it qualitative, quantitative and structural analysis of the
impact is enormous. Way to check wavelength accuracy of many spectral lines as a standard filter praseodymium,
neodymium, holmium oxide filter, holmium oxide wavelength standard solution, deuterium lamp or low pressure
mercury lamp emission and interference filters and so on.
4) Wavelength repeatability: Wavelength Repeatability is the ability of the instrument to return the original
wavelength. It reflects the wavelength drive mechanism and the stability of the whole instrument;
5) Spectral bandwidth (sensitivity, resolution): refers to a peak spectral bands when the slit on the detector
detected through a monochromator energy half-width, expressed in nm wavelength, from another perspective to
understand this concept will more user-friendly: first, the monochromator exit slit represents not just the physical
size or geometry, it also represents the optical sense, this is the spectral bandwidth, we know that the light from the
failure of a single monochromator wavelength, but at a narrow wavelength spectral band are arranged in the order,
the number of spectral wavelength band comprises, represented by the spectral bandwidth. Spectral bandwidth is a
direct response to the quality level of monochromatic light from the monochromator out. The index with the
instrument resolution and sensitivity are similar, but different, they react photometer performance quality from
different sides. Resolution refers to the size of the instrument to distinguish two adjacent wavelengths ability
Sensitivity is measured at low concentrations do, when the concentration changes by one unit to the detector signal
caused by the change amount, it is subject to a calibration curve (standard curve as the horizontal axis, the
absorbance on the vertical axis) and a precision instrument itself restrictions. Two measurement precision of the
method is the same, the greater the sensitivity calibration curve slope, while the slope equal, the higher the
sensitivity, the better precision. It is noted that, in order to obtain accurate test results, the natural bandwidth of the
spectral bandwidth of the instrument (Spectral Bandwidth referred SBW) and the analysis of samples (Natural
Bandwidth referred NBW) ratio should be less than 0.1, more than 99.5% can be obtained so that the measurement
accuracy.

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6) Stray light: wavelength of stray light is irradiated onto the non-selection signal generated by the detector. It is an
important source of photometric analysis of errors, stray light limit the accuracy of the General Assembly high
concentration solution analysis. Stray light represented by T%;
7) Photometric range: Refers to meet the photometric test range in various technical indicators, represented by A or T;
8) Photometric accuracy: the true average value refers to the degree of compliance with a plurality of measured;
photometric accuracy check is usually through the use of repeated measurements of the neutral density filter
carefully film to a standard photometric detection. A neutral density filter for light in a wavelength range having
almost the same transmittance (or absorbance) of the filter, and the use of its wavelength insensitive characteristic
bandwidth changes, to check the accuracy of the optical instrument and repeatability.
9) Photometric Repeatability: refers several times under the same conditions as measured in parallel, each parallel
line with the degree of determination between the results;
10) Noise: the sum of the instrument detects the unwanted signals, which is the purpose of the relative signal.
Generally, the spectrophotometer has two sources of noise, one from the light source, the second is derived from
the internal electronic noise inherent in the instrument, such as: power supply, amplifier, AD conversion and the like.
To reduce noise, improve signal to noise ratio, there must be a good electrical design. Noise measurement
repeatability test under low concentrations, but also affect test accuracy. Noise by averaging several measurements
after partial elimination;
11) Drift: refers to the degree of deviation from the instrument over time the starting value. It depends on the
stability, light stability of the electrical device and the like. Single-beam instrument, the warm-up time has a great
influence on the length of the drift.
12) Baseline flatness: refers to the distribution of the full wavelength range of the instrument noise.
3. Application
Subjects available for physics, chemistry, medicine, biology, pharmacology, geology and other scientific research, is
widely used in chemical, pharmaceutical, biological and chemical, metallurgy, light industry, materials,
environmental protection, medicine and other industries and laboratory analysis of the industry's most one
important quality control instruments, is essential for routine laboratory instruments.
4. Using conditions
Equipment should be installed away from the hot or humid environment. The instrument should be used in 16-35 ℃,
45-80% humidity conditions. Please try to stay away from issuing magnetic, electric, high-frequency waves of
electrical devices, do not install the instrument in the air chlorine, hydrochloric acid gas, hydrogen sulfide gas, such
as sulfurous acid gas, corrosive gas Excessive places. Place the instrument table should be smooth, without

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vibrations; nearby fan of the instrument should be left enough space to exhaust smoothly. Instrument is best to use
a single power outlet, power should ensure good grounding. Doing so may result in equipment not working properly.
If the local voltage instability, give the instrument is equipped with power supply. The instrument should avoid
direct sunlight. Avoid dusty environments.
II. Product Features and Installation
1. Features
With low stray light and a high resolution structure of double-beam optical monochromator , the instrument has a
good stability, reproducibility and accuracy of readings.
Not only the instrument has automatically set 0% T and 100% T and other control functions as well as the
concentration of computing and data processing functions of a variety of methods, but also to prevent user errors
with special features, no worries when using . Scientific design, the use of new technologies, the optical, mechanical,
electrical and computer technology combined with organic, both to improve product performance and convenience
for users to use.
Large screen graphic LCD display can display the data and maps. The extensive machine software can complete
quantitative analysis, qualitative analysis, kinetics, DNA/Protein and other tests. Coupled with powerful storage and
printing capabilities, the computer can complete all testing, analysis and data output with no connection with PC.
Optional equipment is also available on the Windows platform running UV Professional user application software,
the instrument has greater functionality.

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2. Performance Indicator
Model C-7000V C-7000UV
Screen 7-inch TFT screen.
Wavelength
Range 320 - 1100nm 190 - 1100nm
Slit Width 2nm 2nm
Wavelength
Accuracy
±0.3nm ±0.3nm
Wavelength
Repeatability ≤0.2nm ≤0.2nm
Photometric
Accuracy
0.3%T (0-100%T) , ±0.002A(0-0.5A) , ±0.004A(0.5-1A)
Photometric
Accuracy
≤0.15%T (0-100%T), 0.001A(0-0.5A), 0.002A(0.5-1A)
Stray Light ≤0.05%T@220nm,360nm
Stability ±0.001A/h@500nm
Baseline Flatness ±0.0015A ±0.001A
Noise ±0.0005A
Working Mode T,A,C,E
Wavelength
Setting Automatic
Photometric
Range 0-200%T, -0.3 - 3A
Detector Solid Silicon Photodiode
Software Optional with scanning function
Printer Optional
Keypad Silicone buttons
Data Port USB
Light Source Tungsten Halogen Lamp Tungsten Halogen/Deuterium Lamp
Power
requirements AC110-220V 50-60Hz
Humidity Range Less than 85%
Shipping
Dimensions
and Weight
770*630*340mm
27kg

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3. Main Functions