PhotosynQ MultispeQ v2.0 User manual

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MultispeQv2.0
Modified:February14,2020
Version:v1.2.24

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MultispeQv2.0
GettingStarted
Charging:BeforeusingyourMultispeQ,chargeitfor8hoursusingthesupplied
micro-USBcable*.Whenpluggedin,thesmallgreenLEDnexttothemicro-USB
portlightsup.Oncetheinstrumentisfullycharged,thelightwillturnoff.
TurnOn:Pressandholdthepower/resetbuttonintherearfor5seconds.
Software:Gotohttps://photosynq.org/softwareanddownloadtheAndroidapp
ortheDesktopapp.
CheckTutorials:BeforeUsingYourInstrumentMakeSureToCheckOutThe
"GettingStarted"TutorialsonhowthoconnecttheInstrumentandtakethefirst
Measurement.
*Powersupplynotincluded.UseacomputerorstandardUSBpowersupply.Charging
timedependsonthepoweroutputofthepowersupply.
About
MultispeQv2.0sideandbackview

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LeafClampButton
Pushdowntheleafclampbuttontoopentheleafclamp.Pushandreleasewithaneven
motionandpreventitfromsnappingback.Incasetheopenandclosingfeelsrough,a
smallamountoflithiumgreasecanbeusedtomaketheopenandclosingmotionmore
smoothly.
PARSensor
ThePARsensorlocatedontopoftheinstrument.Whentakingmeasurements,
dependingonthemeasurementprotocol,thelightintensitymeasuredwillbereplicated
insidetheinstrument.MakesurethePARsensoriscleanandnottocoverorshade,to
preventalteringtheactualintensity.
Seal
TheNitrilesealaroundthelightguideonthemainbodyandtheclampisensuringthe
areameasuredissealedfromoutsidelight.
LightGuide
Thelightguidesaremadefromacrylic.Makesure,itiscleanandpreventclampinghard,
roughmaterialswhichcancausescratches.
Youmayneedtocleanthelightguidesoccasionally.Gentlycleanthelight
guideswithadamp,softcloth.
Donotsubmergeinwater,donotusesolventsordetergentsanddonotuse
abrasiveorroughclothstocleanthelightguides.
IRContactlessTemperature
TheleaftemperaturecanbemeasuredwiththeIRContactlessTemperature.When
takingameasurement,makesurethattheleafiscoveringtheIRsensor.Thesensoris
insidealittlegrooveandnotflushwiththeclampsurface.Theleaformaterialshould
nottouchthethesensor,sinceitcanalterthemeasuredtemperaturereading.
Temperature&rel.HumidityVent
ThetwoventsallowairtobeexchangedinsidetheInstrument.Therearetwosensors
insidetheclamp,oneisclosetotheventsandthelightguidewhilethesecondoneis
locatedontheotherendoftheclamp.Tubescanbeattachedincombinationwitha
pumptoprovideaconstantairflowand/orgasexchange.

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InstrumentActionIndicator
TheActionIndicatorhasthefollowing:
Indicator Function
5s
Instrumentison,andfullycharged.Theindicatorisflashingevery5s
5s
Instrumentison,andbatteryisemptying.Theindicatorisflashingevery
5s
<1s
Instrumentclampopen.Theindicatorisflashingrapidly.
Instrumentisbusy(e.g.Measurement).Theindicatorisconstantlyon.
Power/ResetButton
Thebuttoninthebackallowstheinstrumenttobeturnedonaswellasreset.
Holdthebuttonforatleast5secondstoturntheInstrumenton.Waitforthe
ActionIndicatortostartflashing,toconfirmtheInstrumentisonandbootedup.
Ashortpushonthebuttonwillresettheinstrument,incaseaprotocolcrashes,etc.
WhentheInstrumentisrunning,holdthebuttonfor5seconds,torestartthe
instrument.WaitfortheActionIndicatortostartflashing,toconfirmthe
Instrumentisbootedupagain.
Tip
Makesuretoonlypressthepowerbuttonthroughthesiliconcovering.
Micro-USB
TheMicro-USBportallowstheInstrumenttobechargedandwhenconnectedtoa
computerfordatatransfer.Whenunpluggingthecable,makesurenottobendthecable
upanddown,tonotbreakoftheInstrumentconnector.
AccessoryPort
TheUSB-Cportallowstoattachaccessoryequipment.Whenunpluggingthecable,
makesurenottobendthecableupanddown,tonotbreakoftheInstrumentconnector.
ChargeIndicator
ThelittleLEDinthebackindicates,whentheInstrumentischarging.Whenthebatteryis
fullycharged,theLEDwillturnoff.

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Configuration
MultispeQv2.0measurements
Body Measurements
Size 224.2mmx53.6mmx56.6mm(l,w,h)
TotalWeight 340g(incl.battery)
LightGuideSurface 8mmx8mm(64mm²)
LeafClampOpening 13mm
Safety Ringforleash
Updates FirmwareupdatesthroughApplications
Lights
TherearesetsoffiveLEDseachonthemainbodyandtheleafclamp.
MainBody
# EmissionPeak Model Specifications
1 530nm LED(green),Lumileds,LXZ1-PM01 view
2 655nm LED(red),Lumileds,LXZ1-PA01 view
3 590nm LED(amber),Lumileds,LXZ1-PL01 view
4 448nm LED(blue),Lumileds,LXZ1-PR01 view
5 950nm LED(NIR),OSRAM,SFH4441 view
LeafClamp

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# EmissionPeak Model Specifications# EmissionPeak Model Specifications
6 950nm LED(NIR),OSRAM,SFH4441 view
7 655nm LED(red),Lumileds,LXZ1-PA01 view
8 850nm LED(NIR),OSRAM,SFH4451 view
9 730nm LED(farred),Everlight,ELSH-Q61F1-0LPNM-JF3F8 view
10 820nm LED(NIR),OSRAMSFH4786S view
EmissionSpectraforLEDs
EmissionspectraforLEDsbuildintheMultispeQv2.0-Emissionnormalizedto
maximumemissionpeak
Detectors
TheMultispeQv2.0comeswithtwodetectors.Onecoveringthenearinfraredonthe
mainbody,theothercoveringthevisualrangeontheleafclamp.
# DetectionRange Model Specifications
1 700nm-1150nm Hamamatsu,S6775-01(mainbody) view
3* 400nm-700nm Hamamatsu,S6775(leafclamp) view
*Note
Thedetector3iscoveredwithaBG-18bandpassfilterwithacenterwavelength
around493nm.Ifyouareusingthedetectortodetectsignalsontheedgesof
thedetectionrange,expectthesignalstrengthtobelow.
Sensors

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TheMultispeQv2.0hasasetofsensorstomeasuretheenvironmentalparameters.
Sensor Model Specifications
AmbientTemperature,
Humidity,Pressure(2x) BOSCH,BME280 view
ContactlessTemperature Melexis,MLX90615SSG-DAG-000-
TU view
Accelerometer Freescale,MMA8653FCR1 view
Magnetometer Freescale,MAG3110FCR1 view
HallEffectSensor TTElectronics,OHS3150U view
PARlightsensor AMS-TAOSUSA,TCS34715FN+
700nmlowpassfilter view
Filters
TheMultispeQv2.0hastwofilters,aBandPassfiltercoveringthevisiblelightdetector
(#3)andaLowPassfilterusedinthePARsensor.
Filter Model Specifications
BandPass Schott,BG-181mmthickness view
LowPass UQCOptics,HotMirrorHM-07 view
IndicatorLights
TheMultispeQv2.0hasnowtwoindicatorlights.Onetoindicateifthedeviceischarging
whichislocatednexttothemicro-USBport.Thesecondoneislocatedontoptoindicate
progressorrequiredactions.
LED Model Specifications
ChargingIndicator ---
Progress/ActionIndicator NeoPixel(RGB),SK6812 view
Battery
TheMultispeQv2.0hasaLi-ionbatterywhichcanbechargedthroughthemicroUSB
port.
Battery Model Specifications
Main SoshineLi-ion26650ProtectedBattery:5500mAh3.7V view

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References
Kuhlgert,S.,Austic,G.,Zegarac,R.Osei-Bonsu,I.,Hoh,D.,Chilvers,M.I.,etal.(2016).
MultispeQBeta:atoolforlarge-scaleplantphenotypingconnectedtotheopen
PhotosynQnetwork.R.Soc.OpenSci.3,160592.doi:10.1098/rsos.160592.