PlexBio IntelliPlex IW100 User manual

IntelliPlexTM 1000 πCode Processor
User Manual
PlexBio Co., Ltd.
PlexBio Co., Ltd.
6F-1, No. 351, Yangguang St., Neihu Dist.,
Taipei City 11491, Taiwan
Tel: [phone]．Fax: [phone]
http://www.plexbio.com
A04-016 V05 (RUO)
For Research Use Only. Not For Use in Diagnostic Procedures.

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IntelliPlexTM 1000 Code User Manual - Jan-20
Table of Contents
Chapter 1. Instruction................................................................................................................. 1
1.1 Overview............................................................................................................................. 1
1.2 Warnings ............................................................................................................................. 1
1.3 Symbols............................................................................................................................... 2
Chapter 2. Safety and Regulatory Issues..................................................................................... 3
2.1 Product Description .............................................................................................................. 3
2.2 Regulatory Labels ................................................................................................................. 3
2.2.1 Testing and Certifications .............................................................................................. 4
2.2.2 Safety Precautions........................................................................................................ 4
Chapter 3. IntelliPlexTM 1000 πCode Processor Technical Overview ........................................... 6
3.1 Introduction......................................................................................................................... 6
3.2 Main Characteristics ............................................................................................................. 7
3.2.1 Shaking and Incubation................................................................................................. 7
3.2.2 Washing...................................................................................................................... 7
3.2.3 Fluorescence Labeling................................................................................................... 7
3.3 System Technical Specification ............................................................................................... 8
Chapter 4. Receiving the Instrument .......................................................................................... 9
4.1 Inspect the Instrument Package ............................................................................................. 9
4.2 Inbox Accessories ............................................................................................................... 10
Chapter 5. Operation Procedure............................................................................................... 11
5.1 Startup......................................................................................................................................... 12
5.2 Touchscreen Introduction .................................................................................................... 13
5.3 Predefined Assay Protocols.................................................................................................. 14
5.3.1 RUN the Built-in Assay Kit............................................................................................ 14
5.4 Create a Custom Program .................................................................................................... 18
5.4.1 Custom Assay Parameters ........................................................................................... 19
5.4.2 How to Create/Edit and Run a Customized New Program................................................ 24
5.5 Special Applications ............................................................................................................ 28
5.5.1 SA-PE Solution ........................................................................................................... 28
5.5.2 Wash Buffer............................................................................................................... 28

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IntelliPlexTM 1000 Code User Manual - Jan-20
Chapter 6. System Settings ....................................................................................................... 31
6.1 Maintenance...................................................................................................................... 32
6.1.1 Renew U Tray............................................................................................................. 33
6.1.2 Clean Manifold .......................................................................................................... 35
6.2 Instrument Reset................................................................................................................ 36
6.3 Rinse Time......................................................................................................................... 37
6.4 Fluid Path .......................................................................................................................... 38
6.5 System Update................................................................................................................... 39
6.6 Assay Update ..................................................................................................................... 39
6.7 Date/Time ......................................................................................................................... 40
6.8 Language........................................................................................................................... 40
6.9 Service Mode ..................................................................................................................... 41
6.10 Log Status ........................................................................................................................ 41
6.11 Shutdown ........................................................................................................................ 44
Chapter 7. Maintenance ........................................................................................................... 45
7.1 Recommended Maintenance Schedule ................................................................................. 45
7.2 As Needed Maintenance ..................................................................................................... 46
7.2.1 Clean Fluid Path Tubes with 70% Ethanol ...................................................................... 46
7.2.2 Clean and Disinfected Surfaces .................................................................................... 46
7.2.3 Renew U Tray............................................................................................................. 46
7.2.4 Long-term Shutdown .................................................................................................. 46
7.2.5 Restarting after Long-term Shutdown ........................................................................... 46
7.2.6 Connecting the Components ....................................................................................... 46
7.2.7 Fuse Replacement...................................................................................................... 49
Chapter 8. Troubleshooting ...................................................................................................... 50
Chapter 9. Contact Information ................................................................................................ 52
Chapter 10. Standard Terms and Conditions for the Use of Products....................................... 56

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IntelliPlexTM 1000 Code User Manual - Jan-20
Chapter 1. Instruction
1.1 Overview
Read this manual carefully before using IntelliPlexTM 1000 πCode Processor. It provides essential
information about the following aspects of IntelliPlex 1000 πCode Processor:
• Safety and Regulatory Requirements
• Labeling
• Technical Information
• Installation
• Operation Procedures
• Settings
• Maintenance 
1.2 Warnings
When operated in a safe environment according to the instructions in this user
manual, there are no known hazards associated with the instrument. However,
the operator should be aware of certain situations that could result in serious
injury or possible damage of the instrument. The protection provided by the
instrument may be impaired if it is used in a manner not specified in this
instruction.

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IntelliPlexTM 1000 Code User Manual - Jan-20
1.3 Symbols
The following symbols may appear throughout this manual with safety warnings, identification,
conditions, instructions and regulatory agencies.
General cautions and
warnings
Caution: pinch point
Warning: Biohazard
Warning: Hot Surface
Manufacturer
Date of Manufacture
Consult instructions for use
Batch code
Serial number
ETL Mark
Federal Communications
Commission (FCC) Mark
Waste Electrical and
Electronic Equipment
For research use only
Table 1. List of symbols

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IntelliPlexTM 1000 Code User Manual - Jan-20
Chapter 2. Safety and Regulatory Requirements
Please read the safety information in this chapter before using IntelliPlex 1000 πCode Processor.
The system contains electrical and mechanical components which may result in injury and damage
of the instrument if handled improperly. Do not perform procedures on IntelliPlex 1000 πCode
Processor that are not specifically described in the user manual.
2.1 Product Description
IntelliPlex 1000 πCode Processor is a 4-in-1 workstation and is intended to be used with dedicated
96-well plates for sample washing, shaking, incubation and automated fluorescence labeling. The
workstation is specifically designed for the πCodeTM MicroDisc hybridization, washing and labeling
steps that precede fluorescent detection by the PlexBioTM 100 Fluorescent Analyzer. Additionally,
this workstation can be utilized for ELISA assay washing if carried out in matching 96-well plates.
2.2 Regulatory Labels
The following label appears on the IntelliPlex 1000 πCode Processor. It displays the model, serial
number, power requirements, regulatory status, electrical safety certification and manufacturer
information.
Fig. 1. Label of IntelliPlex 1000 πCode Processor

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IntelliPlexTM 1000 Code User Manual - Jan-20
The following symbols may also appear on the instrument and user manual as necessary.
2.2.1 Testing and Certifications
The IntelliPlex 1000 πCode Processor has been tested and complies with the safety requirements.
2.2.2 Safety Precautions
The following symbols indicated that safety precautions should be taken before and during system
operation.
This message is used to indicate general caution.
Please handle with extreme care when moving or relocating system as mishandling may
damage the system.
Lift the unit from the base only. DO NOT lift by or put excessive force on the plastic case.
DO NOT put excessive force on the stage or it may become tilted or bent. This will severely
interfere with the performance of the system.
This message is provided to avoid electric shock or possible damage
to the system.
DO NOT open or remove the covers on the system peripherals.
Use only power cords supplied with the system or cords with grounded outlets properly rated
for the system. Ensure the power cord is connected to properly grounded AC outlets only.
Use of surge protector is recommended.
Users must not perform any maintenance or cleaning of the electrical components in the
system.
If any malfunction happens, please turn off the power button and then disconnect the power
cable. The main plug is used as the main disconnection device; avoid positioning the
equipment so that it is difficult to unplug.
Electrical
Cautions
General
Cautions
(FCC mark)

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IntelliPlexTM 1000 Code User Manual - Jan-20
The IntelliPlex 1000 πCode Processor has modules that move during operation. To avoid any
part of the body being caught by a moving part, please keep hands away from the moving
part and do not put your hands under the moving part.
Please do not touch the heating plate surface until it has cooled.
Human and animal samples in the 96-well plate may contain biohazardous, infectious
materials. Please use personal protective equipment (PPE) including gloves and laboratory
coats. Please handle with extreme care when loading the plate onto the stage to prevent
spilling. Refer to Chapter 7 for cleaning instruction.
Within the European Union, the Waste Electrical and Electronic Equipment Directive
2002/96/EC requires the properly dispose of electrical and electronic equipment when it
reaches the end of its lifecycle.
If you are disposing an IntelliPlex 1000 πCode Processor, follow local regulations and requirements.
Pinch
Point
Waste Electrical
And Electronic
Equipment
(WEEE)
Biohazard
Hot Surface

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IntelliPlexTM 1000 Code User Manual - Jan-20
Chapter 3. IntelliPlexTM 1000 πCode Processor
Technical Overview
This chapter introduces and describes the technology, module characteristics and technical
specifications of the IntelliPlex 1000 πCode Processor.
3.1 Introduction
IntelliPlex 1000 πCode Processor is an automated workstation specifically designed for πCode
MicroDiscs hybridization, washing and labeling steps that precede fluorescent detection by
PlexBio 100 Fluorescent Analyzer. The IntelliPlex 1000 πCode Processor minimizes user’s hands-
on time and supports set up of up to six custom protocols (from hybridization to fluorescence
labeling).
Features Include:
User-friendly interface with embedded processing modes for PlexBio assays or customization.
Multifunctional, automated operation that combines aspiration, buffer dispensing, shaking,
rinsing, heating, with magnetic force application to minimize πCode MicroDiscs loss during
washing steps.
Cross-functional capability for both molecular and immunoassays.
7-inch industrial touchscreen panel.

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IntelliPlexTM 1000 Code User Manual - Jan-20
3.2 Main Characteristics
The IntelliPlex 1000 πCode Processor automates the shaking, incubation, washing and
fluorescence labeling steps for assays utilizing πCode MicroDiscs. The characteristics are provided
as follows:
3.2.1 Shaking and Incubation
This function is designed for incubation of compatible 96-well microplates at different
temperatures while shaking.
Features:
Easy to control time and temperature for operation
Simultaneous display of set and actual time, and temperature
Heating and shaking platform for compatible 96-well microplates
Ready to run built-in PlexBio assay programs
3.2.2 Washing
This function is designed to reduce hands-on time and human error during the πCode MicroDiscs
washing process.
Features:
Combines aspiration, buffer dispensing, shaking, automatic rinsing, and the use of magnetic
forces to minimize πCode MicroDiscs loss during washing steps
Simultaneous display of the current washing cycles and status
Convenience: easy for operation and maintenance
3.2.3 Fluorescence Labeling
This function is designed to reduce hands-on time for the fluorescence labeling process. For
compatibility with the PlexBio 100 Fluorescent Analyzer, use of Streptavidin-Phycoerythrin (SA-PE)
conjugates for πCode MicroDiscs fluorescence labeling is recommended.

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IntelliPlexTM 1000 Code User Manual - Jan-20
3.3 System Technical Specification
Model
IW100
PERFORMANCE
Supported plates
96-well plate
(Plexbio; Cat. No. 80025 or Greiner Bio-one; Cat. No. 655101)
Processing time (full plate)
3 ~ 6.5 min (one-time wash)
Shaking
Timing setting range: 1 sec ~ 23hr 59min 59sec
Max. rate: 1200rpm
Incubator
Temperature range from RT+5°C to 60°C
Safety
Plate position sensor and door sensor
Temperature accuracy
± 0.5°C
Temperature uniformity
1°C
Shaking accuracy
≤10%
Dispensing accuracy
≤ ± 6%
(Measured under following conditions: 8-way manifold, 150
μL, PlexBio Wash Buffer)
Dispensing uniformity
≤4% CV
(Measured under following conditions: 8-way manifold, 150
μL, PlexBio Wash Buffer)
MicroDisc loss
Around ≥ 75% of MicroDiscs retained after whole processes.
(Measured under following condition: 500 ~ 1500 discs/well in
150 μL PlexBio Wash Buffer)
Residual volume
≤5 μl/well
(Measured under following conditions: 8-way manifold, 150
μL, PlexBio Wash Buffer)
OPERATION CONDITIONS
Temperature/Humidity
18°C ~ 32°C (64°F ~ 90°F)/20 ~ 80% RH, non-condensing
Altitude
Up to 2000 meters (6561 ft) above mean sea level
STORAGE CONDITIONS
Temperature/Humidity
-10°C ~ 70°C (14°F ~ 158°F)/10 ~ 80% RH non-condensing
PHYSICAL CHARACTERISTICS
Power
100-240V, 50-60Hz, 250W
Dimensions
Without Bottle Carrier: (W)408mm*(D)540mm*(H)470mm
With Bottle Carrier: (W)554mm*(D)540mm*(H)470mm
Weight
38 kg (84 lbs)
Fuse rating
T 5A H 250V
OTHERS
Pollution degree
2
Method of disposal
Electronic waste
Table 2. System Technical Specification

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IntelliPlexTM 1000 Code User Manual - Jan-20
Chapter 4. Receiving the Instrument
Note that IntelliPlex 1000 πCode Processor must be installed and serviced by PlexBio’s qualified
service engineers only. Before installation, please make sure the environmental conditions meet
the operation requirements:
Indoor use only
Operating temperature: 18°C to 32°C (64°F ~ 90°F)
Operating relative humidity: 20% to 80%, non-condensing
Operating altitude: up to 2000m (6561 ft.) above mean sea level
Storage temperature: -10°C to 70°C (14°F ~ 158°F)
IntelliPlex 1000 πCode Processor needs an operation area of 0.36 square meters and needs
to be placed at least 10 centimeters from the wall for components connection and efficient
cooling.
4.1 Inspect the Instrument Package
IntelliPlex 1000 πCode Processor arrives in a large, corrugated cardboard overpack on skids.
Inspect the shipping box and packaging for signs of damage. In case of damage, please contact
your local distributor or PlexBio Technical Support at [phone] or [e-mail].
Do not open the box. Please schedule an installation appointment with your local distributor or
PlexBio Technical Support.
The package of IntelliPlex 1000 πCode Processor may be too heavy to
lift. Please lift using adequate equipment. Please handle with care.
Cautions
Fig. 2. The IntelliPlex 1000 πCode Processor package

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IntelliPlexTM 1000 Code User Manual - Jan-20
4.2 Inbox Accessories
The inbox accessories are listed below. All accessories will be inspected during installation process.
Do not open the box before installation.
Fig. 3.1 Accessories in Top EPE foam packaging
Fig. 3.2 Accessories in the box
Tubes*3
Manual*1
Accessory
box*1
Waste
bottle*1
Bottle
holder*1
Buffer
bottle*2
U Tray*10
V Tray*10
Power
cord*1
AC fuse*2
Hex key 2.0MM*1
Hex key 2.5MM*1
Needle*2

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IntelliPlexTM 1000 Code User Manual - Jan-20
Chapter 5. Operation Procedure
Only operate the IntelliPlex 1000 πCode Processor after installation. Do not operate IntelliPlex
1000 πCode Processor if annual maintenance is overdue.
Ensure all daily/weekly/monthly maintenance is performed (refer to Chapter 7. Maintenance
for details). Not performing required maintenance will void the warranty. Moving the IntelliPlex
1000 πCode Processor is not allowed. If moving is required, please contact Customer Service of
PlexBio Co., Ltd. or qualified local distributors.
Before operation, connect the power cord and the corresponding bottles and tubes (Fig. 4).
Visually confirm that the U Tray is placed in washing tank (Fig. 5, please also refer to section 6.1.1).
Make sure the bottles contain enough ddH2O and enough wash buffer (refer to section 5.5.2), and
the waste bottle is empty.
Fig. 4. Connected diagrams
Fig. 5. Tray position
Front: SA-PE Tank (V Tray)
Back: Washing Tank (U Tray)

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IntelliPlexTM 1000 Code User Manual - Jan-20
5.1 Startup
Turn on the power at the backside of the instrument. The system will run temperature check and
system self-test every time the instrument is powered on. Make sure the ambient temperature is
appropriate for instrument operation, the buffer/water bottles, and the waste bottle is empty.
Click OK to complete system self-test process.
Fig. 6. Interface of system self-test

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IntelliPlexTM 1000 Code User Manual - Jan-20
5.2 Touchscreen Introduction
Figure 7 below depicts the main home screen controls.
(1) Four main function buttons for different assay types.
- Built-in PlexBio Molecular Assay
- Built-in PlexBio Immunoassay
- Built-in PlexBio Applied Science
- Custom Program
(2) Menu button to access settings and maintenance procedures.
(3) Current date and time display.
(4) General information and software versions.
Fig. 7. IntelliPlex 1000 πCode Processor Home Screen
1.
2.
3.
4.

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IntelliPlexTM 1000 Code User Manual - Jan-20
5.3 Predefined Assay Protocols
The IntelliPlex 1000 πCode Processor is installed with predefined assay protocols for the use with
PlexBio’s assay kits.
5.3.1 RUN the Built-in Assay Kit
For running a PlexBio assay kit, please follow step 1 to 6.
Step 1. Fig. 8: Select built-in PlexBio assay type (Molecular Assay, Immunoassay or Applied
Science).
Step 2. Fig. 9: Select the desired lanes for processing and click Next ( ).
Step 3. Fig. 10: Select the corresponding assay to operate.
Step 4. Fig. 11: Confirm the displayed procedure conditions are correct and click START.
Step 5. Fig. 12: Make sure the pop-up window notices are followed and click RUN to start the
assay.
Step 6. Fig. 13: The touchscreen will display real-time operation status of the assay until the
run is complete.
Fig. 8. Select the assay types

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IntelliPlexTM 1000 Code User Manual - Jan-20
Fig. 9. Select the desired lanes for processing
Fig. 10. Select the corresponding assay
One Lane

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IntelliPlexTM 1000 Code User Manual - Jan-20
Fig. 11. Procedures confirmation
Fig. 12. Pop-up window confirmation

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IntelliPlexTM 1000 Code User Manual - Jan-20
Fig. 13. Real-time display of the assay operation status