ThermoFisher Scientific invitrogen iSort Series User manual

For Research Use Only. Not for use in diagnostic procedures.
iSort™Automated Cell Sorter
Catalog Number A33041
Publication Number MAN0016718
Revision A.0

Manufacturer: Cytonome | 9 Oak Park Dr. | Bedford, MA 01730
The information in this document is subject to change without notice.
DISCLAIMER: TO THE EXTENT ALLOWED BY LAW, THERMO FISHER SCIENTIFIC AND/OR ITS AFFILIATE(S) WILL NOT BE LIABLE FOR
SPECIAL, INCIDENTAL, INDIRECT, PUNITIVE, MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION WITH OR ARISING FROM
THIS DOCUMENT, INCLUDING YOUR USE OF IT.
Revision history:MAN0016718
Revision
Date
Description
A.0 19 October 2018 New user guide
Important Licensing Information: These products may be covered by one or more Limited Use Label Licenses. By use of these
products, you accept the terms and conditions of all applicable Limited Use Label Licenses.
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Trademarks: All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. Kimwipes is
a registered trademark of Kimberly-Clark Corporation. Linksys is a registered trademark of Belkin International, Inc.
© 2018 Thermo Fisher Scientific Inc. All rights reserved.

iSort™Automated Cell Sorter User Guide 3
Contents
About this guide ...................................................................................................................... 6
1. Product information .......................................................................................................... 7
Product description......................................................................................................................................... 7
Standard items included................................................................................................................................. 7
Required materials not supplied ................................................................................................................... 8
Instrument exterior components ................................................................................................................... 9
Graphical user interface (GUI)..................................................................................................................... 14
2. Prepare site for installation .............................................................................................15
Site preparation workflow ........................................................................................................................... 15
Site preparation checklist ............................................................................................................................. 15
Pre-installation requirements ...................................................................................................................... 16
Operating environment and site requirements......................................................................................... 17
Safety requirements ...................................................................................................................................... 18
Materials required for installation .............................................................................................................. 19
Prepare for installation ................................................................................................................................. 19
Set up............................................................................................................................................................... 20
3. Startup .............................................................................................................................21
Before you begin............................................................................................................................................ 21
Start up the system........................................................................................................................................ 25
Run Initial Cleaning ...................................................................................................................................... 26
Run Performance Test................................................................................................................................... 28
4. Analyze and Sort ..............................................................................................................31
Prepare samples............................................................................................................................................. 32
System preparation ....................................................................................................................................... 33
Load sample ................................................................................................................................................... 35
Analyze or Sort sample................................................................................................................................. 38
5. Manage files.....................................................................................................................46
Select files ....................................................................................................................................................... 46
Manage files ................................................................................................................................................... 47

4 iSort™Automated Cell Sorter User Guide
6. Run maintenance functions..............................................................................................51
Maintenance screen....................................................................................................................................... 51
Debubble......................................................................................................................................................... 52
Rinse ................................................................................................................................................................ 53
Fluidic Flush................................................................................................................................................... 54
Damper Flush................................................................................................................................................. 55
Cleaning Cycles ............................................................................................................................................. 56
Initial Cleaning ....................................................................................................................................... 57
Sample Line Cleaning ............................................................................................................................ 58
Sheath Fluidics Cleaning....................................................................................................................... 61
Calibration and Performance Test screen .................................................................................................. 66
Performance Test ........................................................................................................................................... 67
Drop Delay Calibration ................................................................................................................................ 68
Flow Rate Calibration ................................................................................................................................... 74
Gimbal Adjustment....................................................................................................................................... 78
Tubing Life ..................................................................................................................................................... 82
System Update............................................................................................................................................... 84
View Log......................................................................................................................................................... 86
Remote Service............................................................................................................................................... 87
7. Shut Down........................................................................................................................88
Daily Shut Down ........................................................................................................................................... 88
8. Settings............................................................................................................................92
9. Instrument care and maintenance ...................................................................................94
Planned maintenance schedule ................................................................................................................... 94
General care.................................................................................................................................................... 96
Cleaning procedures ..................................................................................................................................... 96
Remove and clean the nozzle tip................................................................................................................. 98
Replace peristaltic pump tubing ............................................................................................................... 103
Replace sample pump tubing ............................................................................................................. 104
Replace sheath pump tubing .............................................................................................................. 110
Replace fluidics filters................................................................................................................................. 117
Appendix A: Troubleshooting ...............................................................................................119
Error messages and warnings.................................................................................................................... 124
Nozzle clogs ................................................................................................................................................. 124
Fluidics issues .............................................................................................................................................. 125
Automation issues....................................................................................................................................... 125
Other issues .................................................................................................................................................. 125
Appendix B: Technical specifications ...................................................................................126
Appendix C: Ordering information........................................................................................128

iSort™Automated Cell Sorter User Guide 5
Appendix D: Safety ...............................................................................................................129
Safety conventions used in this document............................................................................................... 129
Symbols on instruments ............................................................................................................................. 130
Safety labels on instruments ...................................................................................................................... 132
General instrument safety .......................................................................................................................... 133
Chemical safety............................................................................................................................................ 134
Chemical waste safety................................................................................................................................. 135
Electrical safety ............................................................................................................................................ 136
Physical hazard safety ................................................................................................................................ 137
Biological hazard safety.............................................................................................................................. 137
Safety and electromagnetic compatibility (EMC) standards................................................................. 138
Documentation and support.................................................................................................139
Obtaining support ....................................................................................................................................... 139

6 iSort™Automated Cell Sorter User Guide
About this guide
Audience
This user guide is for laboratory staff operating, maintaining, and analyzing data
using the Invitrogen
™
iSort
™
Automated Cell Sorter.
User attention
words
Two user attention words appear in this document. Each word implies a particular
level of observation or action as described below.
Note: Provides information that may be of interest or help but is not critical to the
use of the product.
IMPORTANT! Provides information that is necessary for proper instrument
operation, accurate installation, or safe use of a chemical.
Safety alert words
Three safety alert words appear in this document at points where you need to be
aware of relevant hazards. Each alert word—CAUTION, WARNING, DANGER—
implies a particular level of observation or action, as defined below:
CAUTION! – Indicates a potentially hazardous situation that, if not avoided,
may result in minor or moderate injury. It may also be used to alert against
unsafe practices.
WARNING! – Indicates a potentially hazardous situation that, if not
avoided, could result in death or serious injury.
DANGER! – Indicates an imminently hazardous situation that, if not
avoided, will result in death or serious injury. This signal word is to be
limited to the most extreme situations.

iSort™Automated Cell Sorter User Guide 7
1. Product information
Product description
iSort
™
Automated
Cell Sorter
The Invitrogen™iSort™Automated Cell Sorter (Cat. No. A33041) is a compact,
automated cell sorter designed for simplicity and affordability. The system contains
a 488-nm laser and is optimized for sorting cells or particles expressing GFP.
The iSort™Automated Cell Sorter features an easy-to-use touch-screen interface that
requires minimal training. By automating the complex presort setup, the iSort™
Automated Cell Sorter creates a more efficient and consistent workflow.
The small footprint allows the self-contained iSort™Automated Cell Sorter to fit
within a standard biosafety cabinet or on a basic laboratory cart for portability.
Product use
For Research Use Only. Not for use in diagnostic procedures.
Standard items included
The iSort™Automated Cell Sorter is shipped with the system components listed
below. All components are shipped at ambient temperature.
•
iSort™Automated Cell Sorter
•1 sheath fluid bottle
•1 waste bottle
•1 accessory box (located in the instrument box), containing:
-Power cables
-Power supply
-1 replacement nozzle tip and cover
-1 torque wrench
-6 O-rings
Note: For a list of replacement parts for the iSort™Automated Cell Sorter, see
“Appendix C: Ordering information”, page 128.

8 iSort™Automated Cell Sorter User Guide
Required materials not supplied
The following table lists the materials recommended for use with the iSort™
Automated Cell Sorter. The sample and collection tubes listed have been tested
with the system. Unless otherwise indicated, all materials are available through
thermofisher.com.
MLS: Fisher Scientific (fisherscientific.com) or other major laboratory supplier.
Item Purpose Supplier Catalog No.
50-mL Falcon™tube Collection tube Fisher Scientific 14-432-22
5-mL Falcon™Round-Bottom
Polypropylene tubes Sample and Collection tube Fisher Scientific 14-959-11A
1.5-mL Tube (non-sterile) Collection tube VWR 89000-288
1.5-mL Tube (sterile) Collection tube VWR 89000-290
Falcon™Test Tube with Cell
Strainer Snap Cap Sample preparation Fisher Scientific 08-771-23
Flowmi™Cell Strainers for
1000-µL Pipette Tips Sample preparation Bel-Art™SP Scienceware™H136800040
Fisher Scientific 14-100-150
Partec CellTrics™Filter 30 µm Sample preparation Sysmex 04-0042-2316
Partec CellTrics™Filter 50 µm Sample preparation Sysmex 04-0042-2317
PBS (1X), pH 7.4 (flow cytometry
grade)
Sheath fluid, sample
preparation Thermo Fisher Scientific A1286301
PBS (10X), pH 7.4 Calibration and QC Thermo Fisher Scientific 70011044
Tween™20 (50% solution) Calibration and QC Thermo Fisher Scientific 003005
Attune™Performance Tracking
Beads Calibration and QC Thermo Fisher Scientific 4449754
Attune™Wash Solution Daily Shut Down Thermo Fisher Scientific A24974
Decon™Contrad™70 Liquid
Detergent
Nozzle tip cleaning and
storage Fisher Scientific 04-355-01
Distilled water Preparation of solutions Thermo Fisher Scientific 15230162
Deionized water, reagent grade Instrument cleaning Thermo Fisher Scientific 751610
Bleach (without additives and
perfumes)
Fluidics deep clean and
decontamination MLS MLS
Texwipe™TX761MD Microdenier
Swabs Cleaning of optical elements Thermo Fisher Scientific A36701
Porta PlumeSafe™604 Smoke
Evacuation System
(optional)
Biosafety and aerosol
management when sorting
cells under BSL-2 conditions
Buffalo Filter PPS604
Ultrasonic cleaning bath Nozzle tip cleaning MLS MLS
Analytical scale (with 4 decimal
places)
Sort performance verification
during installation MLS MLS
Pipettes (10-µL, 200-µL, 1000-
µL)
and pipette tips Sample preparation MLS MLS

iSort™Automated Cell Sorter User Guide 9
Instrument exterior components
Front view
Touch-screen monitor Sorting compartment
Power button Fluidics compartment
USB port, Type A Sample pump compartment
Front view with sorting compartment door open
Front view with fluidics compartment door open

10 iSort™Automated Cell Sorter User Guide
Rear view
HDMI port (see Important note below) Ethernet connection port
USB ports, Type A (2×) Power input port
USB port, Type B Air exhausts
IMPORTANT! The HDMI port is used by Technical Service representatives to
monitor system performance with diagnostic tools. We do not recommend
attaching an external monitor to the iSort™Automated Cell Sorter via the HDMI
port as this might compromise instrument performance.
Rear view with fluidics compartment door partially open
Rear view with fluidics compartment door fully open

iSort™Automated Cell Sorter User Guide 11
Sorting
compartment
Nozzle assembly
Laser interrogation chamber
Deflection plates
Streamwatch camera window
Collection tray (shown in out position)
Collection tube holder (shown with two 12 × 75-mm tubes with 5-mL capacity;
can hold any combination of 1.5-mL, 5-mL, or 50-mL tubes)
Collection tubes (5-mL tubes shown)
Sort chamber door (shown open)
Sample tube holder (shown in down position)
Sample tube (12 × 75-mm tube with 5-mL sample capacity)
Sample input port (SIP)
Note: The sample input port (SIP) is used for loading samples for Analysis or Sort
modes, and for injecting solutions during maintenance and cleaning cycles.
For instructions on how to load your samples to the iSort™Automated Cell Sorter
for Analysis or Sort, see page 35.

12 iSort™Automated Cell Sorter User Guide
Sample pump
compartment
Note: The front of the instrument is facing to the left.
Top cover
Sample pump dampener
Sorting compartment door Sample pump
Sample line
Rear view of instrument with the top cover open, showing the sample pump compartment

iSort™Automated Cell Sorter User Guide 13
Fluidics
compartment
Waste fluid lines (yellow) Sheath fluid pump assembly
Waste container Fluidics compartment door
(contains fluidics lines, filters, and the
sheath pump
Sheath fluid container
Sheath fluid lines (clear)
Fluid containers
(detail)
Waste container (2 L capacity) Sheath fluid container (2 L capacity)
Waste fluid line with quick
release connector
Sheath fluid line with quick release
connector
Note: For instructions on how to refill or empty the fluid containers, see page 23.

14 iSort™Automated Cell Sorter User Guide
Graphical user interface (GUI)
The iSort™Automated Cell Sorter utilizes a graphical user interface (GUI) accessed
by a touch-screen monitor. The integrated iSort software is pre-installed on the
instrument.
GUI layout and
Home Screen
Key functions of the software (Sort, Analyze, Files, Maintenance, and Settings) are
displayed on the home screen. These functions contain all menus and controls
necessary to execute the selected function.
Sort:Allows you to perform a one-way or two-way sort. For a detailed
description of the Sort workflow, see page 31.
Analyze:Analysis (or non-sorting experiment) is designed for quick
verification of sample content and can be performed independently of the Sort
workflow. For a detailed description of the Analyze workflow, see page 31.
Files:Allows you to manage data files, which are saved in the FCS 3.0 format
after a sort or analysis run. For a detailed description of the Files screen and
how to save and manage data files, see page 46.
Maintenance:Allows you to execute various instrument maintenance
functions, including cleaning cycles, instrument calibration, and QC analysis.
For a detailed description of the Maintenance functions, see page 51.
Settings:Allows you to start and stop fluid flow to the instrument, to set stop
criteria (i.e., number of events) for analysis runs, and to review calibration
and system information. For a detailed description of the Settings menu, see
page 92.

iSort™Automated Cell Sorter User Guide 15
2. Prepare site for installation
Site preparation workflow
IMPORTANT! Do not install the iSort™Automated Cell Sorter yourself. A Thermo
Fisher Scientific representative will contact you to schedule the installation.
When the installation is scheduled:
1. Receive and inspect the system (page 19).
2. Move the crated instrument to the installation site (page 19).
3. Complete the site preparation checklist before the installation date (page 15).
Timeline and
training
Installation of the iSort™Automated Cell Sorter is performed by a Thermo Fisher
Scientific Field Service Engineer. Once successfully installed, the engineer performs
the necessary installation tests. This entire process takes approximately two days.
During and/or after installation, the Thermo Fisher Scientific Service representative
will walk through basic instrument maintenance functions. Additional training is
provided separately by a Thermo Fisher Scientific Applications specialist.
Site preparation checklist
IMPORTANT! Complete all items in the checklist before the scheduled installation
date. If the site preparation checklist is not complete when the Thermo Fisher
Scientific service representative arrives, the scheduled installation may be delayed.

Site preparation requirement Page
Customer responsibilities have been reviewed and personnel assigned. 16
The installation site is identified and meets requirements: 17
Space and clearance
Environmental
Electrical
Network
Safety
Materials that are needed for installation and operation are available. 19
The system was received and inspected: 19
All items on the shipping list are the same items that were ordered at the time of
purchase.
Any damage to shipping containers was recorded on the shipping containers
The installation site is cleared and ready for instrument installation. 19
The crated instrument and other shipping containers are moved to the installation site. 19

16 iSort™Automated Cell Sorter User Guide
Pre-installation requirements
Personnel Responsibilities
Instrument
maintenance personnel
•Reviews the site preparation guide for safety information and system
requirements.
•Coordinates personnel and tasks.
•Orders required materials.
•Selects the site.
•Reviews checklists with appropriate personnel, then with the Thermo Fisher
Scientific service representative to ensure that the site is properly prepared.
•Receives and inspects the system.
•Schedules the installation and notifies personnel of the installation date.
•Ensures that the site is clear of unnecessary material on the installation day.
•Is available to help the service representative throughout installation.
•Ensures that installation requirements are met for:
-Space at the installation site
-Building clearances
-Temperature and humidity
-Waste collection
-Electrical supply
-Computer
-Required safety equipment (page 18) and installation materials (page 19)
•If possible, move the crated system to the site before the installation date.
•Available to help service representative and laboratory personnel throughout
installation.
•Review safety information.
•Ensure that all customer-provided materials for installation are present at the
site.
•Primary users (responsible for training other users) are available during the
installation, so that they can receive initial training on the instrument.
•Reviews the user guide for safety information.
•Ensures that the required safety practices and equipment are in place.
•Is in the vicinity and available to the Thermo Fisher Scientific service
representative when the service representative is at your facility.
Network or IT specialist
(if the system will be
connected to a network)
•Ensures that one active, tested wireless local area network (WLAN) is in place
for Wi-Fi connection before the scheduled installation date.
•Is available during installation to connect the system to the network. The Wi-Fi
connections will also be used for remote troubleshooting if a problem arises.
Note: The Thermo Fisher Scientific Field Service Engineer will connect the
Wireless Wi-Fi Dongle – Linksys™AE3000 N900 Dual-Band Wireless-N USB
Adapter during installation.

iSort™Automated Cell Sorter User Guide 17
Operating environment and site requirements
•
The iSort™Automated Cell Sorter has a footprint (W × D × H) of
approximately 67 cm × 41 cm × 49 cm (26.5 in × 16 in × 19 in) with the fluidics
compartment door and the top cover closed.
With the top cover open, the height of the instrument is 74 cm (29 in).
With the fluidics compartment door open, the entire system requires a total
bench width of ~135 cm (53.2 in).
•The setup for the iSort™Automated Cell Sorter is shown below (top down
view). The gray rectangles in the diagram represent the four handholds in the
base that are used when lifting the instrument out of the box.
Touch-screen monitor
Sorting compartment
Fluidics compartment
IMPORTANT! Do not lift the iSort™Automated Cell Sorter from the fluidics
compartment. Lifting from the fluidics compartment can damage the instrument.
Lift the instrument using the designated handholds.
•
The bench or table top must be able to accommodate 43 kg (95 lbs), the weight
of the instrument plus the weight of the full 2-liter fluid bottles (sheath fluid
and waste).
•The iSort™Automated Cell Sorter should be placed on a level surface away
from vibrations from other pieces of equipment. Tabletop centrifuges, vortex
mixers, and other laboratory equipment can vibrate the instrument during a
run and impact instrument performance.
•The area should be free of excessive dust or moisture. Position the instrument
so that the power button and main power supply can be reached easily.
•Allow at least 6.5 cm (2.5 in) free space at the back of the instrument to allow
for proper ventilation and prevent overheating of electronic components.
•Ambient temperature range: 18°–25°C (64°–77°F)
•Storage temperature range: 5°–35°C (41°–95°F)
•Relative humidity range: 20–70%
•Electrical input: 100–240 VAC, 4.0 A
•
Frequency: 50–60 Hz
IMPORTANT! Cell sorting creates aerosols. Exposure to and inhalation of aerosols
can potentially be hazardous depending on the cell types you are using. Consult
your institution’s safety officer for guidelines related to your individual samples.
The iSort™Automated Cell Sorter may need to be placed in a biosafety cabinet,
depending on your institutions’ safety requirements. The use of a filtration system
(such as the Porta PlumeSafe™604 system from Buffalo Filter; page 8) may also be
required to meet BSL-2 safety requirements of your institution.

18 iSort™Automated Cell Sorter User Guide
Safety requirements
Safety practices
A safety representative from your facility must ensure that:
•Personnel establish and follow all appropriate safety practices and policies to
protect laboratory personnel from potential hazards.
•
All appropriate safety devices and equipment are always available.
Required safety
equipment
The following safety protection and equipment must be available at the
installation site:
•Protection from any sources of hazardous chemicals, radiation (for example,
lasers, radioisotopes, radioactive wastes, and contaminated equipment), and
potentially infectious biological material that may be present in the area where
the Thermo Fisher Scientific service representative will work.
•Appropriate fire extinguisher appropriate for use on electrical and chemical
fires as specified in current codes, regulations, and/or standards, and with
approval of the Fire Marshall or other authority having jurisdiction.
•Eyewash
•Safety shower
•Eye and hand protection
•Sufficient ventilation, including vent line/fume hood, if applicable
•Biohazard waste container, if applicable
•First-aid equipment
•Spill cleanup equipment
•
Applicable Safety Data Sheets (SDSs)
WARNING! BIOHAZARD. Cell sorting creates aerosols. Exposure to and
inhalation of aerosols can potentially be hazardous depending on the cell
types you are using. Consult your institution’s safety officer for guidelines
related to your individual samples.
The iSort™Automated Cell Sorter may need to be placed in a biosafety
cabinet and/or connected to an evacuator filter/pre-filter or liquid
disinfectant trap, depending on your institutions safety requirements.
We recommend that you follow the International Society for the
Advancement of Cytometry (ISAC) guidelines for cell sorter biosafety
standards (www.ncbi.nlm.nih.gov/pmc/articles/PMC4117398/).

iSort™Automated Cell Sorter User Guide 19
Materials required for installation
•
Safety glasses, lab coats, and chemical-resistant, disposable gloves (powder-free)
•Lint-free tissues
•Isopropanol, HPLC-grade or better
•10% bleach solution
•Distilled water
•5-mL and 50-mL Polypropylene Falcon™tubes
•1.5-mL centrifuge tubes
•PBS (1X), pH 7.4 (flow cytometry grade)
•PBS (10X), pH 7.4
•Tween™20 (50% solution)
•Attune™Performance Tracking Beads
•Attune™Wash Solution
•Decon™Contrad™70 Liquid Detergent
•Texwipe™TX761MD Microdenier Swabs
•Pipettes (10-µL, 200-µL, 1000-µL) and pipette tips
•Analytical scale (with 3 to 4 decimal places)
•Ultrasonic cleaning bath (such as Fisherbrand™CPX Series Digital Ultrasonic
Cleaning Bath or similar)
Prepare for installation
Receive and inspect
the shipment
Carefully inspect the shipping containers and report any damage to the Thermo
Fisher Scientific service representative. Record any damage or mishandling on the
shipping documents.
Move the
instrument to the
installation site
1. Clear the installation site.
2. If possible, move the crated instrument and other shipping containers to the
installation site. Do not uncrate.
CAUTION!PHYSICAL INJURY HAZARD. The weight of the iSort™
Automated Cell Sorter is 41 kg (89 lbs). Do not attempt to lift or move the
instrument without the help of others, the use of appropriate moving
equipment, and proper lifting techniques. Improper lifting can cause
painful and permanent back injury. Depending on the weight, moving or
lifting an instrument may require two or more persons
IMPORTANT! Do not subject the iSort™Automated Cell Sorter to sudden impact or
excessive vibration. Handle the instrument with care to prevent damage.

20 iSort™Automated Cell Sorter User Guide
Set up
IMPORTANT! The following set-up instructions are provided for informational
purposes only. Do not attempt the initial set-up of the instrument on your own.
Thermo Fisher Scientific service representative will install the iSort™Automated
Cell Sorter and all of its components and provide some basic operator training.
Place the
instrument at the
installation site
1. After the instrument has been uncrated, verify all parts are present. See page 7
for the list of standard items included in the shipment.
2. Carefully lift the instrument out of the box with the help of at least one other
person, holding it by the four handholds in the base (gray rectangles in the
diagram).
Touch-screen monitor
Sorting compartment
Fluidics compartment
CAUTION! PHYSICAL INJURY HAZARD. The weight of the iSort Automated
Cell Sorter is 41 kg (89 lbs). Do not attempt to lift or move the instrument
without the assistance of others, the use of appropriate moving equipment,
and proper lifting techniques. Improper lifting can cause painful and
permanent back injury. Depending on the weight, moving or lifting an
instrument may require two or more persons
IMPORTANT! Do not lift the iSort™Automated Cell Sorter from the fluidics
compartment. Lifting from the fluidics compartment can cause damage to the
instrument. Lift the instrument only using the designated handholds.
IMPORTANT! Do not subject the iSort™Automated Cell Sorter to sudden impact or
excessive vibration. Handle the instrument with care to prevent damage.
3. Examine the instrument carefully for any damage that may have incurred
during transit. Contact your distributor if anything is missing. Damage claims
must be filed with the carrier; the warranty does not cover in-transit damage.
If you do not have your distributor information, contact Technical Support
(page 139).
4. Place the instrument on the installation site and leave enough room around it
for the hinged doors of the sorting and fluidics compartments to move and
open freely.
Connect the
instrument
1. Confirm that the power is switched off.
2. Plug the power cable into the power supply, then plug the power cable to the
wall outlet. Check for the light on the power supply.
3. Plug the power supply connector into the instrument.
Note: At this point, everything should be plugged in and OFF. Save the packaging
for future shipping/storage of the instrument.