PlexBio IntelliPlex 1000 User manual

IntelliPlexTM 1000 πCode Processor
User Manual
PlexBio Co., Ltd.
PlexBio Co., Ltd.
6F-1, No. 351, Yangguang St., Neihu Dist.,
Taipei City 11491, Taiwan
Tel: [phone]．Fax: [phone]
http://www.plexbio.com
A04-016 V01 (RUO)

Standard Terms and Conditions for the Use of Products
Contract
The terms and conditions herein (hereinafter referred to as the “Standard Terms”) apply to all of
the products and services provided or will be provided by PlexBio Co., Ltd. (hereinafter referred
to as “PlexBio”). Any changes to the Standard Terms shall have no binding effect on PlexBio unless
the same has been agreed upon by an authorized representative of PlexBio in writing. The “Dealer”
referred to herein represents PlexBio (if the products are purchased from PlexBio directly), or the
distributor licensed by PlexBio. PlexBio expressly disagrees with any terms or conditions made by
the buyer in the purchase order or any of such which is different from or not included in this
Standard Terms.
The buyer’s acceptance, unpacking, or use of the products or services shall constitute his/her
acknowledgment and acceptance of the Standard Terms unconditionally. Once the buyer opens
the packaging of PlexBio products or uses the products or services in any manner, the buyer
acknowledges and accepts the Standard Terms unconditionally, and also agrees that the
Standard Terms constitute the contract which has binding effect on the buyer under law. Where
the buyer disagrees with the Standard Terms, he/she shall contact PlexBio immediately to
return the products before using the products or services in any manner, and he/she shall not
use the products or services in any manner. PlexBio has the right to amend the terms and
conditions regarding specification and service without notifying the buyer in advance if no
specification or service has been specified and identified in writing before.
Warranty
The warranty terms and conditions in this Standard Terms (the “Warranty”) apply to any
instruments, spare parts and services (collectively, the “Products”) purchased by the buyer from
PlexBio directly and situated in the territory of Taiwan. The Warranty excludes any activities other
than calibration, certification and maintenance. PlexBio will not provide any Warranty toward the
sale and use of the Products outside the territory of Taiwan. The Products distributed outside the
territory of Taiwan will be sold “As is”. PlexBio will provide the buyer with quality assurance
toward the spare parts purchased from PlexBio and that are used to maintain PlexBio instruments

under the same terms and conditions herein in any countries/territories other than Taiwan
around the world.
Particularly, the Warranty provided herein excludes any products, software or hardware provided
not by PlexBio. If the product is purchased from a PlexBio’s distributor, the Warranty shall be
provided by the distributor to the buyer in writing directly.
Quality Assurance
Products: Regardless of whether or not the buyer accepts said terms and conditions, for any
Products purchased from PlexBio directly, PlexBio will ensure that the performance of all the
Products meets the product specifications provided by PlexBio within 12 months from the
delivery date on (hereinafter referred to as the quality assurance period). PlexBio is entitled to
take one of the following actions against any defects found and reported during the quality
assurance period: (1) payment refund, or (2) defect repairs or spare parts replacements. The
expenses derived from said actions shall be borne by PlexBio.
Software: PlexBio ensures that all the installed software substantially meets the functions
described in the software documentation provided by PlexBio. Notwithstanding, PlexBio does
not guarantee that the software is error-free or may not be attacked by hackers or viruses. The
quality assurance period of the software is identical with that of the PlexBio product in which the
software is installed.
Service: PlexBio ensures that, where the buyer finds any inconformity of related services and
notifies PlexBio of the same in writing within 30 days after the related services are performed;
PlexBio shall be obligated to provide necessary services, assistance and consultation to correct
the inconformity.
General clauses: The Warranty and quality assurance provided are subject to the following
limitations, including (1) the said quality assurance does not apply to consumable materials,
accessories, normal wear and tear, damageable parts and fragile parts; (2) when the buyer asks
PlexBio to provide quality assurance work beyond normal working hours, PlexBio shall be entitled
to charge the buyer additional expenses; (3) the said quality assurance does not apply to the
following circumstances: accidents, modifications, unfair use, abuse, destruction, or disassembly
without authorization, or if the buyer fails to keep or operate in the manner as required, and(or)
do unauthorized maintenance, installation or service, and(or) incorporates or integrates any
product without permission from PlexBio into the PlexBio Products, or integrates the PlexBio
Products into the buyer’s environment or products, and(or) uses other software or user interface

provided by the buyer/vendor without PlexBio’s permission; (4) for the Products sold by PlexBio
but produced by another manufacturer, the quality assurance provided by PlexBio is only valid in
the residual period in which the manufacturer provides its quality assurance; (5) once any
Products is maintained and repaired by PlexBio, the buyer acknowledges that the maintenance
work will not extend the quality assurance period or derive any new quality assurance period.
Other than the Warranties and quality assurance explicitly provided in writing in this Standard
Terms, PlexBio does not provide any other warranties, including but not limited to the warranty
toward fitness for a specific purpose, explicitly or implicitly. If the dealer determines, on the basis
of its own judgment, that the buyer has misused the Products or failed to follow the instructions
to use the Products, the warranty provided by the dealer toward the sale of the Products shall be
invalid.
PlexBio shall not be liable for any direct or incidental damage caused by the use of or failure to
use the Products, including but not limited to, the loss in the process of operation, shutdown,
loss of revenue or profit, loss of the buyer’s product or other products, in addition to the liability
to be borne by the buyer to the supplier, or by the supplier due to such loss, and the labor or
other expenses, damages or losses caused by such Products, including personal injury or loss of
property, unless the personal injury or loss of property is caused by the PlexBio’s willful conduct
or gross negligence.
Maintenance: This maintenance clause applies to Products provided by PlexBio within the
territory of Taiwan only. The maintenance includes correction, verification, and regular
maintenance works only. PlexBio does not provide any warranty to the sales outside of Taiwan.
The Products sold outside Taiwan shall be labeled “original manufactured” and provided “as-is”.
In regards of any spare parts placed outside Taiwan and used for the purpose of maintaining the
PlexBio Products, PlexBio shall provide the quality assurance hereunder.
For any Products situated outside the territories of Taiwan for which maintenance is required:
(i) The buyer shall notify PlexBio in writing of the issue of the Products immediately and also
provide the details about the issue verified.
(ii) The buyer shall contact PlexBio or the service maintenance engineers trained and qualified
by PlexBio to evaluate the problems and verify the issues, and might need to bear the related
expenses derived from the maintenance or transportation of the Products.
(iii) The buyer shall return the Products at issue to PlexBio or the distributor as per PlexBio’s
request. PlexBio might analyze the Products returned. Upon verifying that no defects exist,
PlexBio will send the Products back to the buyer and the buyer shall bear the related
expenses and freight. Notwithstanding, if PlexBio verifies that some issues exist, PlexBio will

bear the freight. The buyer is not entitled to return the Products without PlexBio’s prior
written consent.
Notice to User
The use of this instrumentation and its associated software is covered by one or more
pending US and foreign patents owned by PlexBio Co., Ltd. The purchase of this instrument
and software includes nontransferable rights to use the instrument and software to practice
the methods described therein. No general patent or other license of any kind other than
this specific right of use from purchase is granted. For further information on purchasing
licenses for other applications can be obtained from PlexBio Co., Ltd. 6F-1, No. 351,
Yangguang St., Neihu District, Taipei City 11491, Taiwan.
Trademarks
, ,DeXipherTM, IntelliPlexTM, DigiPlexTM, πCodeTM , PlexBioTM, are
trademarks or registered trademarks of PlexBio Co., Ltd. All other product names and any
registered and unregistered trademarks mentioned are used for identification purposes only
and remain the exclusive property of their respective owners.
Copyright
© 2017 PlexBio Co., Ltd. All rights reserved.

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IntelliPlexTM 1000 Code User Manual - Jul-17
Table of Contents
Chapter 1. Instruction ..........................................................................................................................1
Overview..........................................................................................................................................................1
Warnings ..........................................................................................................................................................1
Symbols............................................................................................................................................................2
Chapter 2. Safety and Regulatory Issues............................................................................................ 3
Intended Use ...................................................................................................................................................3
Regulatory Labels............................................................................................................................................3
Testing and Certifications....................................................................................................................................................... 4
Safety Precautions .................................................................................................................................................................... 4
Chapter 3. IntelliPlex 1000 πCode Processor Technical Overview...................................................6
Introduction.....................................................................................................................................................6
Main Characteristics .......................................................................................................................................7
Shaking and Incubation .......................................................................................................................................................... 7
Washing......................................................................................................................................................................................... 7
Fluorescence Labeling............................................................................................................................................................. 7
System Technical Specification ......................................................................................................................8
Chapter 4. Installation Procedure .......................................................................................................9
Inspect the Instrument...................................................................................................................................9
Instrument Unpacking................................................................................................................................. 10
Unpack Procedures................................................................................................................................................................ 10
List of Accessories .................................................................................................................................................................. 13
Instrument Assembling ............................................................................................................................... 14
Removing the Shipping Brackets...................................................................................................................................... 14
Place the Solution Trays....................................................................................................................................................... 16
Connecting the Components............................................................................................................................................. 17
Chapter 5. Operation Procedure...................................................................................................... 18
Initial Startup................................................................................................................................................ 18
Touchscreen Introduction ........................................................................................................................... 19
Predefined Assay Protocols......................................................................................................................... 20
RUN the Built-in Assay Kit................................................................................................................................................... 20

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Create a Custom Program ........................................................................................................................... 24
Procedure Introduction ....................................................................................................................................................... 25
How to Create a Customized New Program................................................................................................................ 29
How to Overwrite a Program ............................................................................................................................................ 32
Special Applications..................................................................................................................................... 33
Silicone Pad Assembly (Optional).................................................................................................................................... 33
SAPE Solution Supplement................................................................................................................................................. 36
Chapter 6. System Setting................................................................................................................. 37
Maintenance................................................................................................................................................. 38
Renew Tank............................................................................................................................................................................... 39
Clean Manifold........................................................................................................................................................................ 41
Machine Reset.............................................................................................................................................. 42
Rinse Time .................................................................................................................................................... 43
Fluid Path ...................................................................................................................................................... 44
System Update ............................................................................................................................................. 45
Assay Update................................................................................................................................................ 45
Date/Time..................................................................................................................................................... 46
Language....................................................................................................................................................... 46
Service Mode................................................................................................................................................ 47
Log Status...................................................................................................................................................... 47
Shutdown...................................................................................................................................................... 48
Chapter 7. Maintenance ................................................................................................................... 49
Recommended Maintenance Schedule ..................................................................................................... 49
As Needed Maintenance............................................................................................................................. 50
Long-term Shutdown Situation ........................................................................................................................................ 50
Fuse Replacement.................................................................................................................................................................. 50
Chapter 8. Contact Information ....................................................................................................... 51
Customer Service and Sales ........................................................................................................................ 51

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IntelliPlexTM 1000 Code User Manual - Jul-17
Chapter 1. Instruction
Overview
Read this manual carefully before using IntelliPlex 1000 πCode Processor. It provides essential
information about the following aspects of IntelliPlex 1000 πCode Processor:
• Safety and Regulatory Issues
• Labeling
• Technical Information
• Installation
• Operation Procedures
• Setting
• Routine Maintenance 
Warnings
When operated in a safe environment according to the instructions in this user
manual, there are no known hazards associated with the instrument. However,
the operator should be aware of certain situations that could result in serious
injury or possible damage of the instrument. The protection provided by the
instrument may be impaired if it is used in a manner not specified in this
instruction.

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IntelliPlexTM 1000 Code User Manual - Jul-17
Symbols
The following symbols may appear throughout this manual with safety warnings, identification,
conditions, instructions and regulatory agencies.
General cautions and
warnings
Caution: pinch point
Warning: Biohazard
Warning: Hot Surface
Manufacturer
Date of Manufacture
Batch code
Serial number
Waste Electrical and
Electronic Equipment
Consult instructions for
use
ETL Mark
Federal Communications
Commission (FCC) Mark
Table 1. List of symbols

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IntelliPlexTM 1000 Code User Manual - Jul-17
Chapter 2. Safety and Regulatory Issues
Please read the safety information in this chapter before using IntelliPlex 1000 πCode Processor.
The system contains electrical and mechanical components which may result in injury and damage
of the instrument if handled improperly. Do not perform procedures on IntelliPlex 1000 πCode
Processor that are not specifically described in the user manual.
Intended Use
IntelliPlex 1000 πCode Processor is a unique, user-friendly 4-in-1 workstation and is intended to
use for 96-well plate washing, shaking, incubation and automated fluorescence labeling. The
workstation is specifically designed for πCodeTM MicroDisc during the hybridization, washing and
labeling steps required before fluorescent detection by PlexBioTM 100 Fluorescent Analyzer to get
optimized results. Additionally, this workstation can also be applied for ELISA washing only.
Regulatory Labels
The following label appears on the IntelliPlex 1000 πCode Processor. It displays the model, serial
number, lot number, power requirements, electrical safety certification and information of
manufacturer.
Figure 1. Label of IntelliPlex 1000 πCode Processor

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IntelliPlexTM 1000 Code User Manual - Jul-17
The following symbols may appear as necessary on the instrument and user manual which
indicate the information notes and warnings.
Testing and Certifications
IntelliPlex 1000 πCode Processor has been tested and complies with the safety requirements. The
following agency mark will be on the label.
(ETL mark) (FCC mark)
Safety Precautions
In any situation in which user may encounter following symbols, please read and understand all
the safety precaution notes before the system operation.
This message is used to provide general caution information.
Please handle with extreme care when moving or relocating system as mishandling may
damage the system.
Lift the unit from the base only. DO NOT lift by or put excessive force on the plastic case.
DO NOT put excessive force on the stage or it may become tilted or bent. This will severely
interfere with the performance of the system.
This message is provided to avoid electric shock or possible damage
to the system.
DO NOT open or remove the covers on the system peripherals.
Use only power cords supplied with the system or cords with grounded outlets properly rated
for the system.
Ensure the power cord is connected to properly grounded AC outlets only.
Users should not perform any maintenance or cleaning of the electrical components in the
system.
If any malfunction happens, please turn off the power button and then disconnect the power
cable. The main plug is used as main disconnection device, not to position the equipment so
that it is difficult to unplug the mains plug.
Electrical
Cautions
General
Cautions

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IntelliPlexTM 1000 Code User Manual - Jul-17
The IntelliPlex 1000 πCode Processor has modules that move during the operation. The risk
of pinching hazards are presents. Please keep hands away from the moving part and do not
put your hands under the moving part.
Please do not touch the heating thermos plate surface until it has cooled.
Biohazard
Human and animal samples in the 96-well plate may contain biohazardous, infectious
materials. Please use personal protective equipment (PPE) including gloves and laboratory
coats. Please handle with extreme care when loading the plate onto the stage to prevent
spilling.
Within the European Union, the Waste Electrical and Electronic Equipment Directive
2002/96/EC requires the properly dispose of electrical and electronic equipment when it
reaches the end of its lifecycle.
If you are disposing of a IntelliPlex 1000 πCode Processor, please contact PlexBio Technical Support
for a Return Material Authorization (RMA) number at [phone].
Waste Electrical
And Electronic
Equipment
(WEEE)
Pinch
Point
Hot Surface

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IntelliPlexTM 1000 Code User Manual - Jul-17
Chapter 3. IntelliPlex 1000 πCode Processor
Technical Overview
This chapter describes the technology introduction, module characteristics and technical
specifications of IntelliPlex 1000 πCode Processor.
Introduction
IntelliPlex 1000 πCode Processor is the workstation specifically designed for πCode MicroDisc
during the hybridization, washing and labeling steps required before fluorescent detection by
PlexBio 100 Fluorescent Analyzer which minimizes user’s hands-on time. Users could also create
their own custom protocols from hybridization to fluorescence labeling by adjusting the related
parameters.
Features Include:
Easy to use: User-friendly interface with imbedded processing modes for PlexBio assays or
customization.
Multifunctional: Combines aspiration, buffer dispensing, shaking, rinsing, heating, and the
magnetic force application to minimize πCode MicroDiscs loss during washing steps.
Applicability: Designed for both molecular and immunoassays.
Touchscreen: Includes a 7-inch industrial touch panel.

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IntelliPlexTM 1000 Code User Manual - Jul-17
Main Characteristics
The multifunctions of IntelliPlex 1000 πCode Processor are including shaking , incubation, washing
and automated fluorescence labeling optimized for πCode MicroDisc. The characteristics are
provided as follows:
Shaking and Incubation
This function is designed for shaking standard 96-well microplates in thermostatic mode.
Features:
Easy to control time and temperature for operation
Simultaneous display of set and actual time, and temperature
Heating platform for standard 96-well microplates
Built-in PlexBio assay programs with no further setting needed
Washing
This function is designed to reduce hands-on time and human error during the πCode MicroDiscs
washing process.
Features:
Combines aspiration, buffer dispensing, shaking, automatic rinsing, and a magnetic plate for
minimizing πCode MicroDiscs loss during washing steps
Simultaneous display of the current washing cycles and status
Convenience: easy for operation and maintenance
Fluorescence Labeling
This function is designed to reduce hands-on time for the fluorescence labeling process. IntelliPlex
1000 πCode Processor can work with all types of Cy3 fluorescence readers. Use of SA-PE for πCode
MicroDiscs fluorescence labeling is most recommended.

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IntelliPlexTM 1000 Code User Manual - Jul-17
System Technical Specification
Model
IW100
PERFORMANCE
Processing time
(full plate)
3~6.5 min (one time wash)
Shaking
Timing setting range: 1min~23 hr59 min59 sec
Max. rate: 1200rpm
Incubator
Temperature range from RT+5℃to 60℃
Safety
Plate position sensor and door sensor
Temperature accuracy
± 0.5°C
Temperature uniformity
1°C
Shaking accuracy
≤10%
Dispensing accuracy
≤ ± 6%, Measured under following conditions:
8-way manifold, 150 μL, PlexBio Wash Buffer (PBST)
Dispensing uniformity
≤4% CV, Measured under following conditions:
8-way manifold, 150 μL, PlexBio Wash Buffer (PBST)
MicroDisc loss
Around ≥ 75% of microdiscs retained after whole processes,
Measured under following condition:
500~1500 discs/well in 150μL PlexBio Wash Buffer
Residual volume
≤5μl /well, Measured under following conditions:
8-way manifold, 150 μL, PlexBio Wash Buffer
OPERATION CONDITIONS
Temperature/ Humidity
18℃~32℃/ 20~80%RH, non-condensing
Altitude
Up to 2000 meters (6561 ft) above mean sea level
Others
Reliability tests
STORAGE CONDITIONS
Temperature/ Humidity
-10℃~70 ℃/ 10~80% RH non-condensing
Others
Reliability tests
PHYSICAL CHARACTERISTICS
Power
100-240V, 50-60Hz, 250W
Dimensions
Without Bottle Carrier: (W)540mm*(D)408mm*(H)456mm
With Bottle Carrier: (W)540mm*(D)554mm*(H)456mm
Weight
38 kg
Fuse rating
T 5A H 250V
OTHERS
Pollution degree
2
Method of disposal
Electronic waste
Table 2. System Technical Specification

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IntelliPlexTM 1000 Code User Manual - Jul-17
Chapter 4. Installation Procedure
This chapter provides the detailed installation procedures. Note that IntelliPlex 1000 πCode
Processor should be installed and serviced by PlexBio’s qualified service engineers only. Before
setting up and unpacking the processor, please make sure the operation environmental
requirements:
Environmental Conditions
Indoor use only
Operating temperature: 18°C to 32°C
Operating relative humidity: 20% to 80%, non-condensing
Operating altitude: up to 2000m (6561 ft) above mean sea level
Storage temperature: -10°C to 70°C
Inspect the Instrument
IntelliPlex 1000 πCode Processor arrives in a large, corrugated cardboard overpack on skids.
Inspect the shipping box, packaging, instrument and accessories for signs of damage.
The package of IntelliPlex 1000 πCode Processor may be too heavy to
lift. Please lift the instrument from the bottom and do not pull, push,
or bend the case from the side.
IntelliPlex 1000 πCode Processor needs an operation area of 0.36
square meters. The system also needs to be placed at least 10
centimeters from the wall for components connection and for
efficient cooling.
Cautions
Fig 2. The inside package of IntelliPlex 1000 πCode Processor

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IntelliPlexTM 1000 Code User Manual - Jul-17
Instrument Unpacking
The following information provides the assembling process of the instrument.
Unpack Procedures
To set up IntelliPlex 1000 πCode Processor (Fig. 3 to Fig. 8):
1. Remove the carton buckle from the package box.
2. Remove the boxes containing IntelliPlex 1000 πCode Processor. The instrument is inside a
plastic bag and surrounded by foam inserts attached to a corrugated cardboard insert.
3. Remove the surrounded foam inserts and plastic bag.
4. Place the instrument on a stable and flat bench.
5. Remove two black cushions affixed on the frame of the front door.
Fig 3. Remove the carton buckle from the package box

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IntelliPlexTM 1000 Code User Manual - Jul-17
Fig 4. Remove the upper carton from its package
Fig 5. Remove accessories first then remove the foam inserts from its package

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IntelliPlexTM 1000 Code User Manual - Jul-17
Fig 6. Pulling out IntelliPlex 1000 πCode Processor from its package
Fig 7. The unpacked IntelliPlex 1000 πCode Processor
Fig 8. Remove two black cushions on the front door frame

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IntelliPlexTM 1000 Code User Manual - Jul-17
List of Accessories
Before proceeding with the installation, please check the accessories provided with the
accessories list.
Table 3.1 Accessories in Top EPE
Table 3.2 Accessories in the box
Tubes*3
Manual*1
Accessory
box *1
Waste
bottle*1
Bottle
holder*1
Buffer
bottle*2
U tray*10
V tray*10
AC fuse*2
Silicon
pad*3
Power
cord*1
Silicon
pad
fixture*1