Agilent Technologies 5975 MSD User manual

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SERVICE MANUAL
SIM Direct Inlet Probe
for
Agilent 5975 MSD
and
Agilent 7000 Triple Quadrupole
(Firmware V.1.10)

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Document History
Edition 7075e 10/2010, from Firmware / Software Version V1.10 and dip.v.1.2.
The information contained in this document is subject to change without notice.
SIM GmbH makes no warranty of any kind with regard to this material, including, but not limited to, the
implied warranties of merchantability and fitness for a particular purpose.
SIM GmbH shall not be liable for errors contained herein or for incidental or consequential damages in
connection with the furnishing, performance or use of this material.
Reproduction, adaptation, or translation without prior written permission is prohibited, except as
allowed under the copyright laws.
© Copyright 2010, SIM GmbH. Printed in Germany. All rights reserved.

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CONTENT
1 PARAMETER MENU OF THE CONTROLLER (FIRMWARE V1.10) .......................... 4
2 DIP INSTALLATION PROCEDURE............................................................................ 5
2.1 Replacing the ion source ........................................................................................... 5
2.2 Installing the DIP on the Agilent 5975 MSD ............................................................... 5
2.3 Installing the DIP on the Agilent 7000 Triple Q........................................................... 7
2.4 Connecting the DIP and the GC................................................................................. 8
2.5 Configuration of the DIP-Controller ............................................................................ 9
2.6 Installing the DIP Software......................................................................................... 9
2.7 Insert the ChemStation/MassHunter methods for the DIP.........................................10
2.7.1 5975 MSD: ChemStation methods .........................................................................10
2.7.2 7000 Triple Q MS/MS: MassHunter methods .........................................................10
2.8 Adjustment of the CombiPAL to the MS-DIP System (optional) ................................10
2.9 Push rod performance test........................................................................................10
2.10 Tuning of the DIP-MS System...................................................................................10
2.10.1 Autotune 5975 MSD with DIP.................................................................................10
2.10.2 Autotune 7000 TripleQ MS/MS with DIP.................................................................11
2.11 Controller: Update of the firmware ............................................................................11
3 EXACT POSITIONING OF THE PUSH ROD IN THE ION SOURCE .........................12
4 TEMPERATURE CALIBRATION OF THE CONTROLLER ........................................14
4.1 Menu: General Configuration ....................................................................................14
4.2 Temperature Calibration ...........................................................................................14
5 DIP TEST PROCEDURES ........................................................................................16
5.1 DIP Temperature accuracy test ................................................................................16
5.2 Leak test...................................................................................................................17
5.3 Coupling test: CombiPAL with MSD-DIP and GC (optional, for DIP - 45°type with
5975 MSD) ...........................................................................................................................18
5.4 DIP (EI) Performance test.........................................................................................19
5.5 DIP (PCI) Performance test for DIP/5975 MSD Systems ..........................................22
APPENDIX ...........................................................................................................................24

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1 Parameter Menu of the Controller (Firmware V1.10)
Parameter
Assignment min value max value default
0 1000 (Password) 10 0x7fff 1000
1 Temp. adjustment (0.1°C) -200 200 -23
2 LCD contrast 0 50 20
3 push rod position outside (min pos) -400 -100 -136
4 push rod position ion source (max pos)
300 550 467
5 cooling position 100 500 300
6 max temperature 350 500 400
7 xp*0.1 10 1000 150
8 tn*0.1 1 100 40
9 inj-position -400 -100 -135
10 ion source position 300 500 444
11 temp correction (*0.001) -200 200 -115
12 damp down heating oscillations td*0.1 0 30 10
13 O-ring change position -400 0 -115
14
Option bits: 1=useExStart, 2=ready
position inside the DIP, 4=90°position,
8=continuous operation, 16=smoothing
of temperature, 32=early start,
64= CO
2
cooling
-0x8000 0x7fff 23
15 push rod horizontal position -0x8000 0x7fff 75
16 light barrier change position - - 160
17 tilt angle adjustment - - 12
18 dwell time (in valve after opening to the
ion source; 1min =100, default = 6 s) 0 -001
19 position of light barrier for tube test
(-97 = activated, -1 = deactivated) -97
Additional remarks to parameter 14:
You can combine the items by adding the numbers, e. g. “23” (1+2+4+16) for DIP without autosampler or
“31” (1+2+4+8+16) for DIP with autosampler, “95” ((1+2+4+8+16+64) for DIP with autosampler and CO
2
cooling

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This menu is reserved to qualified service engineers only and needs a password.
Select the parameter menu of the controller in the following way:
1. Press <Enter> and keep it pressed
while pressing <arrow up><arrow down><arrow up><arrow down>
then let off the <Enter> key so that the diagnostic menu appears
press <arrow right> and <Enter> so that the cursor in the parameter menu appears:
the first two numbers represent the parameter number, the selected number is taken
with <Enter>
then the belonging value (to this parameter) will be shown and can be changed
2. Select parameter “00” and enter the password “1000”
3. Change the values of the designated parameter.
2 DIP Installation Procedure
2.1 Replacing the ion source
Please follow the instructions given in the corresponding Agilent Manual to replace the
existing ion source with the provided modified ion source (EI or CI).
2.2 Installing the DIP on the Agilent 5975 MSD
Before installing the DIP System to the Agilent 5975 MSD you have to uninstall the MSD
front panel:
1. After you have
disconnected the required
cables, remove the MSD
front panel carefully.
Please follow the
instructions given in the
5975 MSD manual.
2. Demount the two boards for
the MSD display (see
marking on the left) and put
the MSD front panel aside
and

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3. Remove the DIP front panel
of the DIP System following
the instructions given in the
DIP manual (chapter 7.2).
4. Take the DIP front panel
and mount the display
boards of the MSD onto the
preset positions (see left).
5. Before mounting the DIP
system, remove the glass
plate in front of the MSD ion
source compartment.
6. To mount the DIP system in
front of the MSD, first
deinstall the valve unit by
loosing the two screws (see
marked circles on the left).
Now the adapter plate is
separated and the four
screws are well accessible
for the next step.
7. Take the adapter plate and
mount it in front of the ion
source compartment using
the four screws (see
marked circles on the left,
the screws behind the
motors are not visible on
this photo).
8. Then retighten the valve
unit of the DIP system on
the adapter plate with the
two screws according to the
photo of step no. 6.
9. Replace the original knurled
knob of the MSD valve with
the provided knob for the
DIP System (see marked
rectangle in the photo left).

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10. Mount the push rod cover by reversing the instructions in chapter 7.2 of the DIP
manual. Make sure that all cables are correctly connected.
2.3 Installing the DIP on the Agilent 7000 Triple Q
Before installing the DIP System to the Agilent 7000 Triple Q MS/MS you have to uninstall
the MSD front panel. After you have disconnected the required cables, remove the MS front
panel carefully (please follow the instructions given in the Agilent manual.)
1. Demount the two boards of the MS/MS front panel and then put this two-part front
panel aside.
2. Remove the front panel of the DIP System following the instructions given in the DIP
manual (chapter 7.2).
3. Mount the two boards
onto the preset positions
of the DIP front panel
(see marking on the
right photo)
4. Before mounting the DIP
system, remove the
glass plate in front of the
MS/MS ion source
compartment.
5. To mount the DIP system in
front of the MS/MS, first
deinstall the valve unit by
loosing the two screws (see
marked circles on the left).
Now the adapter plate is
separated and the four
screws are well accessible
for the next step.

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6. Take the adapter plate and
mount it in front of the ion
source compartment using
the four screws (see
marked circles on the left,
the two screws behind the
motors are not visible on
this photo).
7. Then retighten the valve
unit of the DIP system on
the adapter plate with the
two screws according to
the photo of step no. 5.
8. Replace the original
knurled knob of the MSD
valve with the provided
knob for the DIP System
(see marked rectangle in the
photo left).
9. Mount the push rod cover by reversing the instructions in chapter 7.2 of the DIP
manual. Make sure that all cables are correctly connected.
2.4 Connecting the DIP and the GC
Please follow the instructions of the Agilent Manual but pay attention to the difference in
installing the DIP controller as you can see in the figures below:
DIP controller rear panel view:
Power cord receptacle
Cooling outOn/Off
Cooling in
RS 232 Cable
LAN Cable
System CableAPG for Start-Remote-Cable (without autosampler)
or
for Remotemanager (with autosampler)

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connecting diagram GC-MS-DIP Controller
GC MSD
DIP
Controller
Diode Cable
B 0613 59 73
Remotemanager
RM 0002 68 90
Start-Remote-Cable
B 0301 40 02
2.5 Configuration of the DIP-Controller
The IP adress is assigned in the menu “Network configuration “ of the DIP controller (see
MSD DIP Manual, 5.5 Network configuration):
1. Turn on the DIP controller.
2. Select the menu “Network configuration” and scroll with the “arrow up” and “arrow
down” key in the parameters.
3. Set the parameter “DHCP Mode” to “Fixed IP”.
4. Select the parameter “IP adress” with the “Enter” key and change the numbers with
the arrow keys.
5. Skip back to the basic display by pressing the “arrow right” key two times.
Or the IP-adress is assigned automatically if the modus “DHCP” is selected (see MSD DIP
Manual, 5.5):
1. Turn on the DIP controller.
2. Select the menu “Network configuration” and scroll with the “arrow up” and “arrow
down” key in the parameters.
3. Set the parameter “DHCP Mode” to “DHCP” (see).
4. Skip back to the basic display by pressing the “arrow right” key two times.
2.6 Installing the DIP Software
Note: You can find all files with software and default methods on the disc enclosed.
1. Copy the files “DIP.exe, dip.enu, dip.deu” into the new folder “DIP”.
2. Copy the default methods in the folder “DIP\Methods”
3. Open the “DIP”-Software.
4. Click on “File” and open the menu “Config.”.
5. Select the DIP controller with a click on the IP adress.

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2.7 Insert the ChemStation/MassHunter methods for the DIP
Note: You can find all the folders with the default methods on the disc enclosed.
2.7.1 5975 MSD: ChemStation methods
1. Copy the folders “DIP_EI_Q1” and “DIP_CI_Q1” into the methods section of the MSD
ChemStation under “Programme\MSDChem\1\Methods”.
2. optional (with autosampler):
Copy the folders “Injection_GC” and “Injection_DIP” into the methods section of the
MSD ChemStation.
2.7.2 7000 Triple Q MS/MS: MassHunter methods
1. Copy the folders “DIP_EI_Scan.M” (and possibly “DIP_CI_Scan.M”) into the methods
section of the MassHunter software under “MassHunter\GCMS\1\Methods”.
2. optional (with autosampler):
Copy the macros (cycles) “DIP-Liquid-Inj.cyx” and “DIP-Solid-Inj.cyx” into the PAL
section under “MassHunter\GCMS\1\PALDATA”.
2.8 Adjustment of the CombiPAL to the MS-DIP System (optional)
1. Define two new DIP positions for a precise co-operation of PAL and DIP (see DIP-
PAL Manual 6.4.3).
2. Create a macro and a new method in the Cycle Composer Software of the
autosampler (see DIP Manual 6.4.4 and 6.4.5) or copy the files of the folder “Cycle
Composer” into
”C:\Programs\PAL\Cycle Composer\Combi PAL”.
2.9 Push rod performance test
1. Select the method “drive.dip” in the DIP-Software and click“OK”.
2. Check the correct insertion of the push rod into the MSD ion source and the
operational sequence backwards in real and even at the status display of the
software.
3. Select the method “heat.dip” in the DIP-Software and click “OK”. The push rod is
inserted into the ion source and heated up to 400 °C. This temperature is maintained
for 1 minute.
4. Check the correct temperature run via the status display.
2.10 Tuning of the DIP-MS System
2.10.1 Autotune 5975 MSD with DIP
1. Verify that the system has been pumping down for at least 2-4 hours and that the DIP
controller is turned on. The DIP push rod status is “Ready”.
2. In the “Instrument Control view”, select “Perform MS Autotune” from the instrument
menu.
The software will perform an autotune and print out the report.

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3. When the autotune has completed, save it as “ATUNE.U”, and then select Tune
Evaluation from the Qualify menu. The software will evaluate the last autotune and
print a System Verification – Tune report.
4. Verify that there is no air and water in the system.
(If there are peaks at 18, 28, and 32 amu, there may be an air leak in the system.)
2.10.2 Autotune 7000 TripleQ MS/MS with DIP
1. Verify that the system has been pumping down for at least 2-4 hours and that the DIP
controller is turned on. The DIP push rod status is “Ready”.
2. In the “Instrument Control view”, select “MS Tune” from the instrument menu and
select the items “print autotune report” and “save tune file when done”. The software
will perform an autotune, save it as “atunes.ei.tune.xml” (default file name) and print
out the report.
3. Go to “Manual Tune – Vacuum Control” and select “Air And Water-Check”. Print the
screen to document the result.
4. Verify that there is no air and water in the system.
(If there are peaks at 18, 28, and 32 amu, there may be an air leak in the system.)
2.11 Controller: Update of the firmware
1. Switch on the controller.
2. Open the program “CHIPTOOL”
(download link : http://www.beck-ipc.com/bc/download/index.asp?sp=en)
that will find all installed chips with their ID number.
3. Select the chip of the SIM DIP controller and click at the right mouse button: Now you
can select the FTP server and enter the following items and finish this procedure with
the “Connect” button:
User: ftp; Password: ftp, Representation Type: Binary (Image)
4. Now you can see the contents of the chip at one side of the screen (Drive A), and the
contents of one of the other drives at the other side of the screen. Select the drive
with the update-files of the new firmware version.
5. Copy the update files “_t.exe” (for SIM controller) and/or “MSDDIP.hex” (for display
controller) into the A directory of the chip and close the FTP server.
If you have updated the MSDDIP.hex file, you have to contiunue with the following
procedure:
6. Select the SIM DIP controller and click at the right mouse button.
7. Now you can choose “Telnet” as connection type .Enter the following items and finish
the procedure with the “Connect” button:
User: tel; Password: tel; Automatically login
8. In this “TELNET session”, break “_t.exe” with the shortcut “Ctrl Z”.
9. Type “dir” so that the directory of A is displayed.
10. Type “d” and “Enter” into the next command line (A:\>d) so that program “D.exe” is
started (D.exe runs the download of MSDDIP.hex from the DIP controller into the
display controller)
11. When this download is done, the update is finished. Reboot the controller for correct
working.

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3 Exact Positioning of the Push Rod in the Ion Source
This chapter is valid for all DIP systems for the Agilent 7000 Triple Q and for those for the
5975 MSD starting from serial number SN1938 (December 2009) because these systems
are not equipped with an inspection glass any longer:
In case of maintenance work at the push rod or for installing purposes it can be necessary to
reposition the push rod in the ion source. Then use the following procedure:
Note: Vent the MS before starting the following procedure (i.e. turbopump is off and ion
source temperature < 100 °C)
1. Turn on the foreline pump to get the prevacuum.
2. Remove the housing of the push rod and the front panel (see DIP user manual, 7.2.)
but do not disconnect the two cables to the front panel because you have to move the
push rod with the display keys later on!
3. Fix the transportation safety screw completely (shown in the figure next to step no.11,
see arrow).
4. Move the push rod out of the parking position in the valve by pressing the arrow key
“up” (front panel) once. Manually bring the push rod into the vertical (90°) position.
5. Remove the Teflon disc (left
figure) and replace it by a
brass one (right figure). The
marker of the valve plug
should be directed down to
the bottom (see blue arrow
in the right figure)..
6. Carefully take the front panel (still connected to the DIP system) and select the service
mode “Valve” (see DIP user manual, 5.4.).
7. Without the push rod (still in the vertical position), select “valve free” with the
subsequent clicks on the arrow keys “right” and “up” and “enter” for confirmation.

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8. Press the arrow keys to open the
valve (control it by watching the
marker of the valve plug which is
directed forwards to the observer
when the valve is open, see blue
arrow in figure on the left).
9. Take the dummy push rod for the
alignment procedure. While the
original push rod is already in the
vertical position, insert the dummy
into the valve as far as possible. If
this is consistent with the position
in the figure left, positioning of the
push rod in the ion source is correct
and you can skip the steps 11 - 12.
.
10. Otherwise please loose the screws
of the brass block slightly. Now you
can cautiously move the valve block
until you are able to insert the push
rod completely.
11. Fix the screws to adjust the correct
position of the push rod.
12. Remove the dummy and exchange the brass disc with the Teflon disc.
13. Click the push rod down while the valve is still open (marker forwards).
14. Mount the push rod cover and the front panel onto the MSD again and press the arrow
“right” three times so that the valve is closed (!) and initializing of the DIP is started.
15. Check the exact position of the push rod by using the service mode “Auto”.
16. Loose the transportation safety screw (see figure above). Now you can pump down the
MS and then start running the DIP.
transportation safety screw

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4 Temperature Calibration of the Controller
4.1 Menu: General Configuration
This menu is used to select the language (German or English).
Moreover, it is possible to change the operational mode which is reserved to the service
engineers!
1. Select this menu (“General Config”) by pressing the “arrow right” key until the service
menu appears and two subsequent clicks on the “arrow right” key.
2. Press “Enter” and select with the “arrow right” key
a) the language (cursor is on the left side): with the arrow keys “up” and “down” you
can change between “English” and “German”.
b) the operational mode (cursor is on the right side): with the arrow keys “up” and
“down” you can change between “Operate” (normal measuring mode) and “ValMode”
(validation mode).
3. Skip back to the basic display by pressing the “arrow right” key.
Note: The validation mode “ValMode” enables the heating of the push rod outside the
ion source!
Due to the risks of bodily injury and damaging the instrument, this mode is
reserved to qualified service engineers!!!
4.2 Temperature Calibration
1. Select “ValMode” at the DIP Controller (see above and DIP Manual 5.6)
2. Put the temperature sensor of a calibrated thermometer (indicates the actual value)
into the push rod tip and isolate these both.
3. Select the parameter menu of the controller:
Press <Enter> and keep it pressed
while pressing <arrow up><arrow down><arrow up><arrow down>
then let off the <Enter> key so that the diagnostic menu appears
press <Arrow right> and <Enter> so that the cursor in the parameter menu appears:
the first two numbers represent the parameter number, the selected number is taken
with <Enter>
then the belonging value (to this parameter) will be shown and can be changed
4. Select parameter “00” and enter the password “1000” (now all parameter values may
be changed)
5. Select parameter “01” and calibrate the controller to room temperature:
example: actual value (thermometer): 20 °C controller-value: 22 °C
change the value to “-20” and press <Enter>
(algebraic sign “-“ because the controller value is higher than the
actual value, otherwise do not use a sign)
6. Load the DIP method “check_temp.dip.” and start it:
The tip of the push rod is successively heated to 30/150/250/350°C. Please note
each temperature of the controller display at the end of the dwell time.
7. Calculate the deviation percentage of each temperature and average over all
temperatures (for example 8%).

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8. Select parameter “11” in the parameter menu and enter the calculated value in the
following manner:
”-80” and <Enter> (for the above mentioned example, if the value of the controller is
higher than the actual value).
”80” and<Enter> (for the above mentioned example, if the value of the controller is
lower than the actual value)
9. Press <Arrow right> to return to the diagnostic menu and leave it with <Enter>.
10. Change the validation mode (ValMode) to the normal measuring mode (Operate) in
the General Configuration Menu (see above and Manual 5.6).

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5 DIP Test Procedures
DIP Temperature Accuracy Test Evaluates the DIP tip temperature using a probe
with a calibrated thermometer.
Air/Water Check during DIP operation Evaluates the DIP for leaks.
Coupling Test: CombiPal with MSD-DIP
Evaluates the function of the DIP (45°version) and
the GC together with the CombiPal
DIP EI Performance Test Evaluates the performance of the MSD (EI) DIP
using a traceable standard.
PCICH4.U Test (if applicable) Evaluates the performance of the MSD (PCI) using
a traceable calibration standard.
DIP PCI Performance Test
(if applicable)
Evaluates the performance of the MSD (PCI) DIP
using a traceable standard.
5.1 DIP Temperature accuracy test
This procedure uses a traceable digital thermometer to determine the temperature accuracy
of the push rod tip at the DIP system.
CONDITIONS
- MS – “Ready”
- DIP – “Ready/Bereit”
PROCEDURE
1. With the push rod outside, select the “Validation Mode” of the DIP controller:
Go to the menu “General Configuration” (see DIP manual, 5.6 Menu General
Configuration) and change the operate modus to the validation modus “ValMode” with
the arrow keys “up” or “down”.
2. Put the temperature sensor of the calibrated digital thermometer into the tip of the
push rod.
3. Isolate the tip of the push rod together with the thermometer and shut the cap of the
DIP.
4. In the DIP software, select the method “check_temp.dip” and start the temperature
program.
5. When the first setpoint is reached, observe the calibrated thermometer’s display until
the reading stabilizes.
6. Record the thermometer’s reading at the end of the 5 minute’s dwell time in the table
of the Results section.
7. Repeat no. 5 and 6 for the second and third setpoint.
8. In the DIP controller’s “General Configuration” menu change the mode from
“ValMode” to “Operate”.

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RESULTS
DIP Temperature Accuracy Test
Setpoint
[°C]
Measured
Value
[°C]
Typical Range
of Operation Pass/Fail Verified By/Date
30 30 ± 5
150 150 ± 5
250 250 ± 7
350 350 ± 7
5.2 Leak test
This procedure consists of the Tune evalutation results (MS ChemStation) or the Air/Water
Check (MassHunter software) described in section 2.10 (DIP in “Ready” status with the push
rod outside the MS ion source and the results of an Air/Water check while the push rod is
located in the ion source. The leak test of DIP/MS-System is passed, when the results of
both measurements do not essentially differ.
REQUIREMENTS
- an autotune and tune evalutation test (for 5975 MSD) or “Air And Water Check” (for 7000
Triple Q) have just been performed.
CONDITIONS
- MS – “Ready”
- DIP – “Ready/Bereit”
PROCEDURE
1. Start the DIP Software and load the method “a_w_check.dip”.
2. Start the DIP (start button in the software or controller): The push rod will be inserted
into the ion source, heated up to the source temperature (230 °C) and be kept there
for 10 minutes.
3. When the setpoint is reached and the reading stabilizes, perform a tune evaluation or
an “air/water check” (5975 MSD: go to “Instrument / Edit MS Tune Parmeters” and
select “Air/water check”; 7000 Triple Q MS/MS: go to “Manual Tune / Vacuum
Control” and select “Air and Water check”.
4. Compare this result with the tune evaluation report (5975 MSD)/air and water check
(7000 Triple Q MS/MS)when the push rod was in the “Ready” position outside the MS
ion source (see this manual, section 2.10) and complete the table in the Results
section.

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RESULTS
LEAK TEST
Item
Air/Water
Check [%],
push rod outside
ion source
Air/Water
Check [%],
push rod inside
ion source
Limit for deviation
[percentage points]
Pass/Fail Verified By/Date
Abundance
Ratio 18/69 < 5
Abundance
Ratio 28/69 < 2
5.3 Coupling test: CombiPAL with MSD-DIP and GC
(optional, for DIP - 45°type with 5975 MSD)
This procedure checks the function of the CombiPAL autosampler by alternating injections
into the DIP system and the GC.
REQUIREMENTS
- Two additional DIP positions have been defined in the CycleComposer software according
to the DIP manual.
- Cycle Composer: Verify that the methods “GC-Injection” and “DIP-Injection”, the macros
“Injection DIP” and “Injection GC” and the sequence “Injection_GC-DIP” (see “Cycle
Composer” on the disc enclosed) are copied into “Programs\PAL\Cycle Composer\ Combi
PAL.
- MSD ChemStation: Verify that the folders “Injection_GC” and “Injection_DIP” (see
“MSChemstation” on the disc enclosed) are copied into the methods section of the MSD
ChemStation under “Programme\MSDChem\1\Methods“. Moreover, copy the sequence
“Injection GC-DIP” into the sequence section of the MSD ChemStation.
CONDITIONS
- MSD – “Ready”
- MSD-DIP – “Ready/Bereit”
- CombiPAL – Power on
PROCEDURE
1. Position an empty vial on position 1 at tray 1 of the CombiPAL.
2. Open the MSD ChemStation and load the sequence “ Injection GC-DIP (possibly
check it with “edit sequence”).
3. Open the “Cyle Composer with Macro Editor” and load the sample list “Injection_GC-
DIP”.
4. Open the DIP software and download the method “drive.dip”.
5. Switch to the MSD ChemStation window and start the sequence (“run sequence”).
6. Switch to the Cycle Composer window and start the sample list (“start”).
7. Watch the alternating injections into the DIP and the GC (3 times each) and complete
the table below.
The sequence has to run completely with the needle in the correct positions.

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5.4 DIP (EI) Performance test
This test uses a standard solution with two components to determine the correct
performance of the DIP. Perform this procedure on both types of probe tips (for liquid and for
solid samples).
REQUIREMENTS
- An autotune has been performed.
- A new method for DIP EI measurements has been created in the MS ChemStation
software:
Follow the instructions of the MSD ChemStation manual and note that there are some
different inputs to be made:
• In the menu item “Inlet/Injection Types” select for “Sample Inlet” Other/None, for
“Injection Source” External Device, for “Scan Type” MS1 Scan and enter the value for
“Total Runtime”which is displayed in the DIP software (DIP method “check_EI.dip”, see
DIP manual).
- Optional: CombiPAL is installed correctly on the GC/MSD-DIP system (see DIP manual)
CONDITIONS for DIP/5975 MSD
DIP Ready / Bereit
DIP method check_EI.dip (30°C for 0.1 min, 30-350°C at 0.5°/s, 350°C for 5 min)
MSD Ready
MSD method DIP_EI_Q1.M
MSD aux temp 280°C
Tune file ATUNE.U
Aquisition mode SCAN
Run length 15.50 min
Column flow 0.5 ml/min
Solvent delay 0.10 min
EMV Relative ± 0
Mass range (low) 100.0
Mass range (high) 600.0
Threshold: Inst 150
Sampling: 2
MS quad temp: 150°C
MS ion source temp :
230°C
Optional : CombiPAL
PAL method DIP-injection.PME
Sample triglyceride standard
Push rod tip A: for liquid samples, B: for solid samples
Sample volume 1 µl

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CONDITIONS for DIP/7000 Triple Q MS/MS
DIP Ready / Bereit
DIP method check_EI_scan.dip (40°C for 1 min, 40-350°C at 1.0°/s, 350°C for 5
min)
MS/MS Ready
MS method DIP_EI_Scan.M
MS aux temp 280°C
Tune file atunes.ei.tune.xml
Ion Sorce EI
Aquisition mode SCAN
Scan Type MS1 Scan
Stop Mode By Stop Time
Stop time 10 min
Column flow 0.5 ml/min
Solvent delay 0.3 min
Time filter enabled yes
Time filter peak width
0.7 s
Use gain no
EMV Use tune setting (Delta EMV=0)
Mass range (low) 100.0
Mass range (high) 600.0
Scan time 100 ms
Threshold 0
Step size 0.1 amu
MS quad temp
(Q1 + Q2) 150°C
MS ion source temp :
230°C
Sample triglyceride standard
Push rod tip A: for liquid samples, B: for solid samples
Sample volume 1 µl
PROCEDURE
Set up the Methods
1. Load method DIP_EI_Q1.M for the 5975 MSD (MS ChemStation) or DIP_EI_Scan.M
for the 7000 Triple Q MS/MS.
2. Select the actual autotune using Instrument/Select MS Tune File (ATUNE.U or
atunes.ei.tune.xml).
3. Save the method to “…\1\METHODS\” as “DIP_EI_Q1.M” resp. “DIP_EI_Scan.M”
4. Print the method and verify that method and conditions are the same.
5. Load method “check_EI.dip” for the DIP-system (via DIP software, see DIP manual).