POL-EKO SMART PRO ILC 180 User manual

CO2incubators
model: ILC 180
Before using the equipment, please read carefully this instruction manual!
Version 1.11
Issued 20.10.2022
Instruction manual SMART PRO

Instruction manual ILC SMART PRO
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Manufacturer's address:
POL-EKO-APARATURA sp. j.
ul. Kokoszycka 172 C
44-300 Wodzisław Śląski
Country of origin: Polska
As a manufacturer, we inform you that we took the necessary measures to ensure that this device fully meets your
expectations and is reliable for a long period of use. Due to the continuous improvement of our products, as well as the
expansion of our offer, any suggestions regarding additional functions and equipment functioning are welcome. Visit our
homepage www.pol-eko.com.pl/home-en/
Equipment disposal
This equipment is marked with the crossed out wheeled bin symbol to indicate that this equipment must not be disposed
of with unsorted waste. Instead it's your responsibility to correctly dispose of your equipment at lifecycle -end by handling
it over to an authorized facility for separate collection and recycling. It's also your responsibility to decontaminate the
equipment in case of biological, chemical and/or radiological contamination, so as to protect from health hazards the
persons involved in the disposal and recycling of the equipment. For more information about where you can drop off your
waste of equipment, please contact your local dealer from whom you originally purchased this equipment.
By doing so, you will help to conserve natural and environmental resources and you will ensure that your equipment is
recycled in a manner that protects human health.
Thank you!

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Contents:
1. SAFETY INFORMATION................................................................................................................................6
1.1. Avoiding electrical shock ........................................................................................................................7
1.2. General safety instructions on installing and operating the CO2incubator .............................................7
1.2.1. Indications for using pressure cylinders .............................................................................................7
1.2.2. Characteristics of carbon dioxide .......................................................................................................8
1.2.3. Location requirements........................................................................................................................8
1.2.4. Hazards when working with CO2........................................................................................................8
1.2.5. Presonal protection / first aid..............................................................................................................8
1.2.6. User’s responsibility ...........................................................................................................................9
2. PACKAGE CONTENTS..................................................................................................................................9
3. BEFORE THE FIRST USE............................................................................................................................10
3.1. Setting up the chamber.........................................................................................................................11
3.2. Shelf / water pan installation.................................................................................................................12
3.3. Water pan.............................................................................................................................................13
3.4. Remarks on the placement of samples.................................................................................................14
3.5. Closing chamber door...........................................................................................................................14
3.6. Internal glass door................................................................................................................................14
3.7. Connection of the CO2gas cylinder......................................................................................................15
4. DESCRIPTION OF THE unit ........................................................................................................................16
4.1. Design of ILC incubators ......................................................................................................................16
5. UNIT EQUIPMENT........................................................................................................................................17
5.1. Internal glass door................................................................................................................................17
5.2. Door lock ..............................................................................................................................................17
5.3. Access ports for external sensor...........................................................................................................18
5.4. Open door alarm...................................................................................................................................18
5.5. USB port...............................................................................................................................................18
5.6. LAN port ...............................................................................................................................................19
6. DEVICE OPERATION...................................................................................................................................19
6.1. External memory (USB flash drive).......................................................................................................19
6.2. First boot...............................................................................................................................................20
6.3. Using the keypad..................................................................................................................................20
6.4. User logging in......................................................................................................................................21
6.5. Main screen.................................................................................................................................21
6.5.1. Information panel..............................................................................................................................23
6.5.2. The meaning of icons and symbols..................................................................................................25
6.5.3. Upper expandable and configurable menu.......................................................................................27
6.5.4. Quick Note –user’s message...........................................................................................................29

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6.5.5. Alarm bar..........................................................................................................................................29
6.6. Quick Program......................................................................................................................................29
6.7. Programs.....................................................................................................................................31
6.7.1. Creating / editing a program.............................................................................................................32
6.7.2. Segments edition..............................................................................................................................33
6.7.3. Summary of segments......................................................................................................................35
6.7.4. Protection class................................................................................................................................36
6.7.5. Priority..............................................................................................................................................36
6.7.6. Loop .................................................................................................................................................36
6.8. Starting the program.............................................................................................................................37
6.8.1. The first way.....................................................................................................................................37
6.8.2. The second way...............................................................................................................................38
6.9. Quick Change of parameters................................................................................................................39
6.9.1. Quick change of set temperature .....................................................................................................39
6.9.2. Quick change of set time..................................................................................................................40
6.9.1. Quick change of set CO2concentration............................................................................................41
6.10. Schedules...................................................................................................................................42
6.10.1. Creating / editing a schedule............................................................................................................43
6.10.2. Starting a schedule...........................................................................................................................46
6.11. Statistics ......................................................................................................................................48
6.12. Data record..................................................................................................................................49
6.12.1. Data storage directly on a USB flash drive.......................................................................................52
6.13. Event log .....................................................................................................................................52
6.14. Info ..............................................................................................................................................55
6.15. Users ...........................................................................................................................................56
6.15.1. Creating / editing a user ...................................................................................................................57
6.15.2. Account types and their limits...........................................................................................................58
6.16. User settings panel......................................................................................................................60
6.16.1. Unlocking the touch screen..............................................................................................................61
6.17. Time ............................................................................................................................................61
6.18. Alarms.........................................................................................................................................63
6.18.1. STM function....................................................................................................................................64
6.18.2. Mute option.............................................................................................................................66
6.19. Network.......................................................................................................................................66
6.20. E-mail reports ..............................................................................................................................68

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6.21. Corrections...................................................................................................................................70
6.22. Hot-air sterilization program..................................................................................................................71
7. INTERFACE..................................................................................................................................................74
7.1. MODBUS TCP......................................................................................................................................74
8. TEMPERATURE PROTECTION...................................................................................................................75
9. CONNECTING THE DEVICE TO A COMPUTER.........................................................................................75
10. CLEANING AND MAINTENANCE OF THE DEVICE...................................................................................75
10.1. Replacing the CO2gas filter..................................................................................................................76
10.2. Exterior cleaning...................................................................................................................................76
10.3. Interior cleaning....................................................................................................................................76
10.4. Disinfection of the CO2sensor..............................................................................................................77
10.5. Cleaning the touch screen....................................................................................................................77
10.6. Advice on how to safely store the device..............................................................................................78
10.7. Consumables........................................................................................................................................78
11. TROUBLESHOOTING..................................................................................................................................78
11.1. Possible defects....................................................................................................................................79
12. WARRANTY CONDITIONS..........................................................................................................................80
13. RATING PLATE............................................................................................................................................81
14. TECHNICAL DATA.......................................................................................................................................82
15. DECLARATION OF CONFORMITY .............................................................................................................84

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1. SAFETY INFORMATION
ILC SMART PRO CO2incubators offer optimal growth conditions for cell cultures, at approx. 37oC. The unit enables a
CO2 control and high humidity inside the chamber which prevent osmolarity increase.
The incubator can be loaded only with materials and substances which cannot explode, burst or ignite (which do not form
any toxic or explosive vapours at set temperature). Potentially explosive gas-air mixtures can’t be formed, neither in the
working chamber nor in the direct vicinity of the incubator. The unit should not be used to sterilize samples. The
sterilisation program in the menu only sterilizes the inner chamber.
CO2incubator is not classified as a medical device according to Medical Device Directive 93/42/EEC. Do not use it to
sterilise medical devices.
The meaning of information symbols
CAUTION This symbol indicates a potential risk and alerts you to proceed with caution.
CAUTION This symbol indicates important information and the recommendations to follow.
CAUTION This symbol indicates risks associated with hot surfaces.
This symbol means that a given field / window can be moved in the direction shown in the picture.
To guarantee your security and the longevity of the unit, please comply with the following rules:
1.
The unit cannot be installed:
•outside,
•in unventilated recesses,
•in damp places or places which can be easily flooded,
•near flammable or volatile substances (any potentially explosive areas),
•near acids or in corrosive environments.
2.
It is forbidden to:
•store inflammable or explosive substances inside the unit,
•touch the inner door and glass door handles, and the inner surfaces after a sterilization process,
•touch live parts of the unit,
•operate the unit with wet hands,
•put water vessels on the unit,
•climb or put any objects on the unit,
•overload the shelves (the maximum load is described in technical data).
3.
You should:
•ensure sufficient ventilation,
•place samples in such a way to provide proper air circulation in the chamber,
•open the door for the shortest period of time to reduce temperature fluctuations,
•always check that the doors are closed,
•use only earthed mains to avoid electric shocks,
•unplug the power cable holding the protective cover and not the cable itself,
•disconnect the unit from the mains before undertaking any repairs or maintenance works,
•protect the power cable and the plug from any damage,
•disconnect the power plug before moving the unit,

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•disconnect the power plug if the unit will not be used for a long period of time,
•disconnect the unit if it has any visual faults.
Failure to comply with the above recommendations may result in damage to the device or deterioration of
technical parameters, as well as loss of warranty.
1.1. Avoiding electrical shock
The electric installation should meet the following conditions:
The unit is powered by AC 220-240V/ 50-60Hz. It must be connected to an earthed mains socket.
The electric installation should be secured by a circuit breaker 16 A characteristics B.
1.2. General safety instructions on installing and operating the CO2
incubator
1.2.1. Indications for using pressure cylinders
•During the operation of the CO2incubator, small amounts of CO2enter the room which in high
concentration can be life-threatening. The unit does not emit CO2when the power supply is
switched off and the gas cylinder valves are closed.
•The CO2incubator can’t be operated near concentrated acids or corrosive vapours.
•The workplace must be properly ventilated.
•Emergency shutdown of the CO2 dosing: close the gas cylinder valve.
The amount of dispensed CO2is regulated by a microprocessor controller. If the chamber door is open during CO2
dosing, the gas will be automatically cut off.
•Together with a pressure cylinder with compressed gas, the user should be given a safety data sheet for the
gas and instructions for its use.
•The ventilation performance in the room must ensure level of gas concentrations in the room below the limit
values. High concentration of CO2(>4 Vol.-%) - risk of death by suffocation.
•During gas intake from the gas cylinder, check the pressure level regularly.

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1.2.2. Characteristics of carbon dioxide
Carbon dioxide (CO2) is a colorless and odorless gas, therefore it is practically imperceptible. CO2is heavier than air and
soluble in water.
Warning signs on the bottle containing CO2:
Non-flammable and non-toxic gases
Pressurised gas
Product ID: Carbon dioxide (100%)
Chemical formula: CO2
Application of the substance: General industrial applications
1.2.3. Location requirements
To prevent the accumulation of gas above the permissible concentrations in the room, natural or mechanical ventilation
should be provided. Inaddition,a natural or mechanical installation mustbe provided toprevent the oxygen concentration
from falling below 19.5%.
Observe the occupational exposure limit OEL for CO2set by national authorities (formerly maximum permitted workplace
concentration). Check compliance when operating all chambers located in the room.
It is recommended to use a control and measurement equipment for measuring CO2concentration in rooms
with a CO2incubator.
1.2.4. Hazards when working with CO2
Carbon dioxide in elevated concentration causes shortness of breath, circulation problems and
ultimately death. Avoid breathing gas.
The bottle with compressed gas may cause cryogenic burns or injury (extremely cold liquid and/or
high pressure gas).
1.2.5. Presonal protection / first aid
In the event of gas leak:
Leave the room immediately, warn other people and ventilate the room if possible.
Respiratory protection:
In an atmosphere where there is a lack of oxygen (excess of carbon dioxide), use a self-contained breathing apparatus
or a face mask with a positive pressure air supply. Respirators with air filters will not provide protection.
Inhalation problems:

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Move the victim to fresh air. CO2gas is heavier than the air and accumulates e.g. at the floor. If breathing has stopped
or is difficult, give aided respiration. Oxygen administration may be indicated. In the event of cardiac arrest, a trained
person should immediately begin cardiopulmonary resuscitation.
In life-threatening emergencies:
1) protect yourself with a breathing apparatus,
2) remove the victim from the contaminated area,
3) call a doctor,
4) start CPR.
1.2.6. User’s responsibility
The user is obliged to:
1) be familiar and comply with applicable health and safety rules, regulations for handling CO2and to train operators
properly,
2) secure the unit against access by unauthorized persons,
3) keep the unit in perfect condition,
4) follow the maintenance schedule,
5) ensure that operators use appropriate personal protective equipment,
6) share the user manual and CO2 safety data sheet with operators working with the unit.
2. PACKAGE CONTENTS
CO2incubators in SMART PRO version are delivered with:
Description
Quan-
tity
CO2incubator
1
rack for shelves
1
water pan
1
stainless steel perforated shelf
3
key to the lock
2
power cable with EU plug
1
3 m pressure hose with pre-assembled hose nozzle for a quick connector and a hose clamp to connect
the gas hose to the pressure reducer
1
silicone plug to close the access port
2
Lab Desk program (in the internal memory of the equipment)
1
LAN cable [pcs.]
1
Quality Control Certificate [pcs.]
1
Instruction manual (in the internal memory of the equipment)
1
Quick start guide
1
Additional accesories can be ordered separately.
Options description
Image
Quantity
Additional perforated stainless
steel shelf, max shelf load 30 kg
1

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Additional reinforced perforated stainless steel
shelf (max. shelf load 30 kg)
1
Stacking adaptor
1
Base on castors
1
Support frame on castors (height: 800 mm)
1
Replacement CO2gas filter
1
ILC 180 SMART PRO is stackable. Two units can be stacked on top of each other with the stacking adaptor, saving
valuable space in the laboratory.
The stacking adaptor prevents heat transmission, shocks and vibrations transferring from one chamber to another.
The base on castors allows the incubator to be easily moved from one location to another, it is also recommended to
avoid dirt from the floor potentially contaminating the chamber.
The CO2gas filter is a consumable part (see section 10.7).
3. BEFORE THE FIRST USE
The manufacturer sends the device protected by cardboard profiles and foil. The device should be transported in an
upright position and the package should be secured against sliding during transport.
After receiving the device, visually assess its condition and equipment in the presence of the person
delivering the goods. A courier company is responsible for any damage caused during transport.
After transporting the device at a temperature below 10°С, wait at least 2 hours before connecting it
to the mains.
On the surface of unit’s components made of stainless steel, slight discoloration may occur. It is a result of the
technologies used in the production of metal sheet in accordance with the requirements of PN-EN 10088-2 standard and
it is not a defect of the unit.
The place of installation of the unit should meet the following conditions:
•Ambient temperature between +20°C...+30°C
•Ambient relative humidity < 60%
•Low dust environment
•Appropriately ventilated according to its size
•Placed on a hard and stable surface
•Placed at least 100 mm away from the wall