PolyScience 3032 series User manual

3032 MICROSCOPE SERIES
INSTRUCTIONS
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3032
SAFETY NOTES
1.Openthe shipping carton carefully to prevent any accessory, i.e.
objectives or eyepieces, from dropping and being damaged.
2. Do not discard the molded Styrofoam container; the container
should be retained should the microscope ever require
reshipment.
3. Keep the instrument out of direct sunlight, high temperature or
humidity or dusty environments. Ensure the microscope is
located on a smooth, level and firm surface.
4. If any specimen solutions or other liquids splash onto the stage,
objective or any other component, disconnect the power cord
immediately and wipe up the spillage. Otherwise, the instrument
may be damaged.
5. CAUTION: The lamp, lamp house (Fig.1-3) and adjacent parts
will become very hot. Do not touch these parts until they have
completely cooled. Never attempt to handle a hot halogen bulb.
6. All electrical connectors (power cord) should be inserted into an
electrical surge suppressor to prevent damage due to voltage
fluctuations.
7. For safety when replacing the halogen lamp or fuse, ensure the
main switch is off (“O”), remove the power cord, and replace the
halogen bulb after the bulb and the lamp house has completely
cooled.
8. Confirm that the input voltage indicated on your microscope
corresponds to your line voltage. The use of a different input
voltage than indicated will cause severe damage to the
microscope.
9. When moving the microscope, hold the instrument with one
hand on the lower portion of the eyepiece tube (Fig. 1-1) and the
other hand on the illumination bracket (Fig. 1-2)
10. Halogen lamp: 6 volt 30 watts. Do not use a lamp with
different specifications.
Figure 2
Figure 1
1
2
3
4

CARE AND MAINTENANCE
1.Donot attempt to disassemble any component including eyepieces, objectives or focusing
assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal
surfaces should be cleaned with a damp cloth. More persistent dirt should be removed using
a mild soap solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using an air
stream from an air bulb. If dirt remains on the optical surface, use a soft cloth or cotton
swab dampened with a lens cleaning solution (available at camera stores). All optical lenses
should be swabbed using a circular motion. A small amount of absorbent cotton wound on
the end of a tapered stick makes a useful tool for cleaning recessed optical surfaces. Avoid
using an excessive amount of solvents as this may cause problems with optical coatings or
cemented optics or the flowing solvent may pick up grease making cleaning more difficult.
Oil immersion objectives should be cleaned immediately after use by removing the oil with
lens tissue or a clean, soft cloth.
4. Store the instrument in a cool, dry environment. Cover the microscope with the dust cover
when not in use.
5. Microscopes are precision instruments which require periodic servicing to maintain
proper performance and to compensate for normal wear. A regular schedule of
preventative maintenance by qualified personnel is highly recommended. Your authorized
distributor can arrange for this service.
SAFETY SYMBOLS
Symbol Meaning
The surface is very hot. Do not touch with your hands.
Before using, please read the instructions carefully. Improper operation may
result in injury or microscope malfunction.
The main switch is “on”
The main switch is “off”

Lamp House
Phase Annuli Slider
Condenser
Eyepieces
Trinocular Viewing
Head
3032 INVERTED MICROSCOPE SERIES
1
Illumination
Bracket
Stage
Base
On/Off Switch
Variable Light Intensity
Adjustment Dial
Fine Focus Knob
Coarse Focus
Knob
X & Y
Stage
Movement
Controls

2．
．．
．Assembly
3032
2.1 Assembly Diagram
The following figure shows the correct installation sequence of the components. Assemble the
components in the exact numerical sequence as in the diagram.
Inspect all optical surfaces for dust and debris; clean if necessary.
Please save the hexagonal wrench for future use.
Retain all stryofoam cartons if reshipment is ever required.
2
Figure 2
Lamp House (3)
Microscope Base
Stage Extension (6)
Mechanical Stage (5)
Power Cord (11)
Objectives (4)
Glass Stage Plate (7)
Eyepieces (8)
Filter Holder ((10)
Condenser Illumination
Assembly (1)
Color Filter (9)
BNC Electrical Cable (2)

3032
2.2 Assembly Steps
Figure 3
2-2-1 Installing and Replacing the Lamp (Figure 3）
）
）
）



Use the specified halogen lamp: 6V30W
Wrap the bulb (1) with gauze or lint free paper; press the
pins (2) into the socket (3) in the lamp house.
When replacing the lamp turn the main switch to “O” (off)
and remove the power cord. Allow the lamp, lamp house
and the adjacent areas to sufficiently cool before handling.
The lamp will become very hot and will cause burns.


Do not touch the halogen bulb with your hands.
Fingerprints on the bulb may shorten the bulb life or
interfere with the illumination. Clean all fingerprints
with a dry soft cloth.
2-2-2 Installing the Condenser Illumination
Assembly（
（（
（Figure 4）
））
）
1. Insert the condenser illumination unit (1) into the
bracket (2).
2. Turn the condenser illumination unit at clockwise
about 90 until the “AS” mark of filter holder③is
facing forward.
3. Keep the screws the of condenser illumination
unit and the holes of the bracket aligned. Tighten the
screws with the supplied hexagon wrench.
4. Insert BNC connector cable ④into the BNC
connector plug ⑤.
2-2-3 Installing the Lamp House (Figure 5)
Keep the BNC connector plug①and the lamp house
pin②aligned. Also keep the bolt③and the condenser
jack④aligned. Then gently push the lamp house into
the illumination unit until they are completely
connected.
3
Figure 4
Figure 5
3
2
1
4
1
2
3
1
3
5
6
4
2
4

3032
4
Figure 7
Figure 6
Figure 8
2-2-4 Installing the Objectives（
（
（
（Fig.6 & 7）
））
）
1. Turn the coarse focusing knob①clockwise until the
nosepiece reaches its lowest position. The tension
control collar (2) has been factory adjusted.
2. Install the lowest magnification objective into the
nosepiece. Then, in a clock-wise direction, rotate the
nosepiece and install each succeeding higher
magnification objective.
3. When changing objective magnifications, rotate the
nosepiece until you hear a “click” sound. This ensures
the objective is centered in the optical light path.
★Inspect the objectives regularly for dirt and oil; clean if
necessary.
★Cover all unused nosepiece holes with a nosepiece
plug③to prevent dust and contamination from entering.
★Use the 10x objective to initially focus the image of your
specimen.
2-2-5
Attaching the Mechanical Stage and Stage
Extension （
（（
（ Figure 8 ）
））
）
1. Attach the mechanical stage to the right side of the fixed
stage by tightening the two thumb screws on the
underside of the mechanical stage.
2. Attach the stage extension to the left side of the fixed
stage by tightening the two thumb screws (1) on the
underside of the stage extension.
1
3
1
2

3032
2-2-6 Installing the Stage Plate（
（
（
（Figure 9）
））
）
1. Install the glass stage plate ①into the stage opening.
2-2-7 Installing the Eyepieces（
（（
（Figure 10）
））
）
1. Remove the protective caps on the eyepiece tubes①.
2. Insert completely the eyepieces into eyetubes.
2-2-8 Installing the Color Filters (Figure 11)
★Allow the color filter to cool completely before you
change them. Remove the filter holder①,and then
insert the color filter ②required.
● Mount the color filter so it lays flat ③in the holder as
shown.
★The color filter must be laying flat. If not, the filter
has been installed incorrectly④
④④
④.
● More than one filter may be installed only if the total
thickness is less than 11mm.
5
Figure 9
Figure 10
Figure 11
1
2
1
1
2
3

Figure 12
3032
Figure 13
2-2-9 Connecting the Power Cord（
（
（
（Fig.12, 13
and 14）
））
）
★
★ ★
★ Do not place stress or strain on the power
cord. These will damage the cord and cause
a danger to the user.
1. Turn the main switch ①to “O” (off) before
connecting the power cord.
2. Insert the power cord plug ②into the electrical
connector ③.
3. Insert the power cord ④ into an electrical
receptacle (5).
4. Insert the BNC connector plug ⑨into the BNC
connector jack ⑧.
★
★★
★Ensure the power cord is connected to a
grounded receptacle.
Use of an electrical surge suppressor receptacle
is highly recommended.
2-2-10 Replacing the Fuse (Figures 12 & 13）
））
）
Turn the main switch ①to “O” (off) before
replacing the fuse. Unplug the power cord.
Rotate the fuse holder ⑥out of the base ⑦
with a flat edge screwdriver, replace the fuse,
then insert the fuse holder and tighten.
★Fuse rating: 250V，
，，
，500mA.
6
Figure 14
3
2
7
6
8
9
4
5
1

3. Adjustment 3032
7
Figure 15
Diopter ring
Light path selector lever
Light switch
Tension adjustment collar Main switch Coarse focus knob Fine focus knob
Phase contrast slider
Color filter holder
Aperture diaphragm adjust lever
Phase center bolt

4. Adjustments 3032
4-1 Microscope base
4-1-1 Turning on the Lamp（
（
（
（Figure 16）
））
）
Connect the power cord; turn the main switch ①
to “－” (on).
4-1-2 Adjusting the Brightness（
（（
（Figure 17）
））
）
Rotate the variable light intensity dial (Fig. 17-1)
clockwise to increase the brightness. Rotating the dial
counterclockwise reduces the brightness.
Using the lowest feasible brightness will increase
the bulb life.
4-1-3 Adjusting the Tension Adjustment
Collar（
（（
（Figure 18）
））
）
★ The tension of the coarse focus knob ②
②②
② has been
factory adjusted.
Rotate the tension adjustment collar ①in the
direction shown by the arrow (Figure 18) to increase
the tension of the coarse focus knob ②. Rotating the
collar in the opposite direction will decrease the
tension.
The coarse focus knob is too loose if the nosepiece
drops automatically or if the specimen looses focus
soon after focusing with the fine focus knob ③.
Tighten the tension adjustment collar if either of
these occurs.
8
Figure 16
Figure 18
Figure 17
1
2
1
3
1

3032
4-2 Stage
4-2-1 Viewing the Specimen（
（
（
（Figures 19 & 20）
））
）
For optimum viewing, ensure the thickness, as
marked on each objective (0.17mm or 1.2mm) is the
same as your container, dish or slide.
Use the appropriate bracket to hold your Terasaki well
plate (3), culture dish (4), flask (5) or slide (5). A larger
flask may be placed directly on the stage. Slides may
also be placed by securing with the slide holder (2).
The specimen is positioned by turning the X (6) and Y (7)
stage movement controls.
9
Figure 19
Figure 20
1
2
3
4
5
6
7

3032
4-3 Viewing Adjustments
4-3-1
Diopter Adjustment（
（
（
（Figure 21）
））
）
1. Using the 10x objective and your right eye
only, observe your specimen through the right
eyepiece and bring it into focus,
2. Then observe the specimen with your left eye
only through the left eyepiece. If the specimen
is not in focus, rotate the diopter ring① until a
sharp image is obtained.
The diopter range is ±5
4-3-2 Interpupillary Distance Adjustment
（
（（
（Figure 22）
））
）
While observing with both eyes, hold the
left and right prism eye tubes. Rotate the
eye tubes around the central axis. The
interpupillary distance is correct when the
left and right fields of view coincide
completely with one image.
The interpupillary distance range is
48-75mm.
Figure 21
Figure 22
2
3
10

11
Figure 23
1
4-3-3
Switching the Light Path（
（
（
（Fig.23）
））
）
★ The light path desired is obtained by pulling out or
pushing in the light path selector lever ①.
●For binocular observation, push the light path selector lever
“in” completely.
●For trinocular observation, pull the light path selector lever
“out” completely.
Light Path
Selector Lever
Illumination
Proportions
Applications
Pushing in the
lever
completely
100% for
Binocular
observation
Binocular
observation
Pulling out the
lever
completely
20% for
Binocular
observation
and 80% for
Trinocular
observation
Binocular
observation,
video monitor,
computer monitor
and
microphotography
Epi Fluorescent
Models
100%
Binocular
or
100%
Trinocular
Fluorescence
Applications

3032
4-4 Illumination Unit
4-4-2 Using the Aperture Diaphragm（
（（
（Figure 25）
））
）
● When using bright field observation, the aperture diaphragm is used to control the numerical
aperture of the illumination system not the brightness. Only when the numerical apertures of the
objective and the illumination system are equal can higher image resolution, contrast, and
increased depth of field be obtained.
●Generally, when observing the chromatic specimens, the N.A. of the condenser aperture diaphragm
is adjusted to 70-80% of the numerical aperture marked on the objective.
To adjust the aperture diaphragm, remove the eyepiece then looked into the viewing tube. Your field of
view will appear as Figure 25. The proportion may be changed by turning the aperture adjustment
lever: (①is the image of the aperture diaphragm & ②is the edge of the objective).
4-4-1
Using Color Filters（
（
（
（Figure 24）
））
）
Select the appropriate color filter according to the
type of illumination being used. Insert the filter
into the filter holder.
The filter holder can hold multiple filters up to a
total thickness of 11mm or less.
Color
Filter Usage in Microscopy
IF550 Green Filter
use for phase contrast microscopy
LBD
Blue Filter
use for bright field observation and
microphotography
12
Figure 24
Figure 25
1
2
70-80%
30-20%

5．
．
．
．Phase Contrast Microscopy 3032
5-1Nomenclature
5-1-1
Phase
Phase Phase
Phase C
CC
Contrast
ontrast ontrast
ontrast O
OO
Objective
bjectivebjective
bjective（
（（
（F
FF
Fig
igig
igure
ure ure
ure 26
2626
26）
））
）
The microscope’s standard phase objectives are
10x and 20x. They are mounted on the objective
turret as described in 2-2-4.
5-1-2 Phase Contrast Annulus Slider
(Figure 27)
●The phase annulus ring has been centered. The phase
annulus ring may be adjusted by using the centering
telescope as described in 5-2-2.
●The 10X/20X phase ring ① is used with the 10X and
20X phase contrast objectives. The empty opening
②is used for bright field observation.
13
Figure 27
Figure 26
1 2

Phase Contrast Microscopy 3032
5-2 Installation and use
5-2-1 Installing the Phase Contrast Slider（
（
（
（Figure
28）
））
）
1. With the slider ①facing upwards (the surface with
printing), insert it into the illumination system.
2. Brightfield and phase contrast light rings have their
own position. Move the slider until you hear the
“click” to ensure the ring or the opening is centered
in the light path.
3. When observing with a phase contrast objective,
keep the aperture diaphragm adjustment lever ②in
the position “O” (open).
5-2-2Centering Telescope (Figures 29 and 30）
））
）
The phase annulus has been pre-centered.
Adjustments will usually not be required with
normal usage.
If adjustments are required:
1. Place the specimen on the stage and focus as usual.
2. Remove the eyepiece without the diopter adjustment
and replace it with the centering telescope.
3. Ensure sure the matched phase contrast objective and
annulus ring in the phase contrast slider are in the
center of the light path.
4. Using the centering telescope to observe the light
ring’s image ①and the phase contrast ring’s
image②. If the light ring’s image is not sharp, turn
the recessed screws in the phase annulus slider until
you can see a clear image of the light ring②
superimposed on light ring (1). The best image will
be obtained only when the two rings coincide.
5. Changing containers, dishes or slides of different
thicknesses will require re-adjustment of the phase
annulus.
14
Figure 28
Figure 29
Figure 30
3
1
2
2
1

6．
．．
．Microscope Video and Photography 3032
6-1 Microscope Video
14
6-1-1 Selecting the Light Path（
（
（
（Figure 31）
））
）
1. For binocular observation, push the light path selector
lever (1) in completely.
2. Focus your specimen as usual.
3. For video or camera usage, pull horizontally on the light
path selector lever until it is completely pulled out.
6-1-2 Installing the Video Adapter（
（（
（Figure 32）
））
）
1. Loosen the locking thumb screw ①on the trinocular
viewing head and remove the protective cap②.
2. Remove the protective caps on the both ends of the
video adapter③.
3. Screw the threaded end into the CCD/CMOS port and
tighten the thumb screw on the adapter (3).
3. Install the accessories into the vertical port and tighten
the thumb screw ①.
4. Attach your video camera to the video port (4).
6-1-3 Focusing（
（（
（Figure 32）
））
）
Focus on your specimen while observing through the
eyepieces. Ensure the light path selector lever is fully
pulled out. Observe the image on the video or
computer monitor. If the image is not in focus, turn the
revolving video tube④until the image is sharp.
Figure 31
Figure 32
1
CCD
Video
2
3
4
1

3032
6-2 Microscope Photography
15
6-2-1 Selecting the Light Path (Figure 31)
1. For binocular observation, push in completely the
light path selector lever (1).
2. Focus your specimen as usual.
3. For microphotography, pull horizontally on the light
path selector lever until it is completely pulled out.
6-2-2 Installing the Photo Camera（
（
（
（Figure 33）
））
）
1. Loosen the locking thumb screw①on the
trinocular viewing head and remove the
protective cap②.
2. Install the adapter③into the vertical port, and
tighten the thumb screw ①.
3. Insert the camera adapter into ④into the camera
port.
●Camera magnification = objective magnification x
camera lens magnification
★The shutter on some cameras may cause a jarring
impact when photographing through the
microscope. To weaken the impact and obtain a
clear image, select a longer exposure time.
6-2-3 Focusing
Focus as usual in the binocular mode. When using the
microscope for photography, use the camera’s viewfinder
to focus the specimen. Please refer to the camera’s user
manual for additional details.
Figure 33
1
2
3
4
Camera

7.Specifications 3032
Optical
System Infinity Optical System
Viewing
Head
Compensation Free Trinocular Head Inclined 30º
Division ratio: 20% for Binocular Viewing and 80% for Video Imaging &
Microphotography
Eyepiece Wide Field Eyepiece 10X; 22 mm field of view
Nosepiece Reversed Quintuple Nosepiece
Objectives Infinity Long Working Distance Plan Achromatic: 4X & 40X
Infinity Long Working Distance Plan Phase Contrast: 10X & 20X
Focusing
System
Coaxial Coarse and Fine Focusing System
Sensitivity and Graduation of Fine Focus: 0.002mm
Movement Range(from the surface focus of stage plate): up 8mm, down 3mm
Mechanical
Stage
Size: 250mm x 160mm
Movement Range: 120mm（width）×78mm（length）
Illumination Halogen Lamp 6volt 30watt; Variable intensity
Condenser Long working Distance Condenser, N.A. 0.3; Working Distance 72mm
Objective Specifications
TYPE MAGNIFICATION
NUMERICAL
APERTURE
（N.A）
WORKING
DISTANCE
（mm）
CONJUGATE
DISTANCE
（mm）
FOCUS
DISTANCE
（mm）
COVER
SLIP
THICKNESS
Infinity
Long
Working
Distance
Plan
Achromatic
Objective
4X 0.1 25.2 ∞45 ─
40X 0.6 3.2 ∞45 1.2mm
Infinity
Long
Working
Distance
Plan Phase
Contrast
Objective
10X 0.25 11 ∞45 0.17mm
20X 0.4 6 ∞45 0.17mm
16