Leica MS5 User manual

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tems
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and
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The
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Units
in
L
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Microsy
ste
ms
hold
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ma
na
ge
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tem
ce
rtifi
cate
s
for
the
international standards
ISO
9001
and
ISO
14001
re
lating to
qu
a
li
ty
manag
emen
t,
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envir
onme
ntal
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om
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Leica
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anual
MICROSYS
TEMS

D
•·
ar
User
User Manual
Thank
you
for
choosing
our
products
.
We
hope
that
you
will
en-
joy
the
quality
and
performance
of
Le
i
ca
Microsystems
products
.
In
developing
our
instr
uments,
we
have
placed
great
emphasis
on
simp
l
e,
self-explanatory directions.
In
order
to
ut
il
ize
all
the
benefits
of
your
new stereomicroscope,
we
suggest
studying
this
user
manua
l
in
de
t
ail.
Should
you
have
any
questions,
please
consult
your
local
Leica
representative.
You
will
find
the
address
of
the
closest local representati
ve
as
well
as
valuable
informa-
tion
about
products
and
services
from
Leica
Microsystems
on
our
homepage
at
www.leica-microsystems.
com
We
are
gladly
at
your
service.
Customer
service
is
a
big
thing
with
us
.
Not
only
before
the
sale,
but
afterwards
as
well.
Leica
Microsystems (Switzerland)
Ltd
Business
unit Stereomicroscopy
www.stereomicroscopy.
com
Your
instrument
is
accompanied
by
a
printed
English
user
man-
ual.
Additional
language
versions
and
information
can
be
found
on
the
interactive
CD-ROM
.
User
manuals
and
updates
are
availab
lefor download
on
our
homepage
at
www.stereomicroscopy.
com
.
This
user
manual
contains
an
explanation
of
the
safety regula-
tions,
assembly,
handling
and
accessories
of
the
Leica
MS5,
MZ6,
MZ75,
MZ9
s,
MZ12
s,
MZ16
and
MZ16
A(if identical) stereo-
microscopes.
The
special functions
of
the
automated
stereo-
microscope
Leica
MZ16
A
can
be
found
in
the
separate
user
manual
M2-116-0
.
Leica
M Stereomicroscopes
User
Manual
3

Table
of
eontent
s
Page
Overview
Illuminators
Safety
co
nce
pt
...........
.
..
.
....
..
...
.
..
..
.
..
...
.
.. ..
..
l:i
Transfo
r
mers
....
.
..
..
....
....
..
......
..
.
..
....
...
......
38
Symbols
............
..
.
..
..
..
..
.. . .
....
.
......
.
...
......
9 Incident
lamp
6
V/10
W
....
..
..
....
...
..
......
......
.
...
.
40
Controls
andfunctions
..
.. .. . .
....
...
...
..
....
.
..
..
.. .
..
10
Incident
lamp
6
V/20
W ...
.....
.
..
..
...
.
..
..
........
..
.. .
42
Cold
light
sources
....
.
...
.
........
.
..........
.
....
..
..
.
45
Use
Fiber-optic light
guide
....
...
.
....
.
..
.
..
..
.
.....
.. .
.....
.
46
Changing
the
magnification
........
....
..
.
..
........
..
...
12
LED
illum
i
na
t
ion
..
...
.....
..
......
....
......
..
...
..
...
..
46
Ergonomics
..
...
.......
..
.
......
..
....
...
....
.. ..
.
..
...
14
Supply
(line)
lamp
25
W .
..
.
..
..
..
.....
......
.
..
......
.
..
47
Interpupilla
ry
distance
........
....
.....
..
.....
.
...
.. .
...
15
Coa
x
ial
i
ll
uminator
.. .
..
...
.
....
.
.....
.
....
.
..
..
.......
..
47
Eye
point . .
..
... ..
........
.
....
..
... .
..
..
....
.. . . ..
...
.
15
Near
-vertical illuminator
...
..
... .
......
.. .
..
....
.. ..
...
..
48
Eye
contact,
eyec
u
ps
.
.....
.
.....
.
..
.
.. ..
..
.
...
...
..
..
..
16
Focusing
..
.......
.
.........
....
.
....
.
..............
.
..
16
Accessories
Microscope carrier:Stereoscopic
and
axial
observati
on
.
..
.
18
Fitting
accessory
tubes
...
..
.
.......
.. ...
.... ..
.
....
...
..
50
Diopte
rsettings
............
.
...
.
....
..
........
.
........
20
Double-iris
diaph
r
agm
....
..
..
..
....
..
.
.....
...
.
..
.....
.
50
Drawing
tube
,di
scussion
tube
.... ..
....
...
..
.........
...
51
Assembly
Video/phototubes:
Overview
:
Components
.. ..
..
..
.....
.... .
...
.. ..
..
..
...
..
22
Photograp
h
y,
video,
TV,
filming, digital
imaging
.
..
...
....
...
52
Attachment
fo
r vertical
and
oblique
observation
.....
.
....
..
56
Stand
Graticules
.............
.. .
.........
...
..........
.
....
..
56
Profile
column
for
incident light
and
transmitted
-light
stands
..
23
Stages,
po
l
arization
..
......
..
..
....
....
..
...
......
...
...
57
Transmitted-li
ght
stand,
bright
fie
l
d,
20
W ..
...
.
.....
..
.....
24
Transmitted-li
ght
s
tand,
bright
and
dark
field
...
....
. .. . .
...
26
Special
notes
Transm
itted-li
ght
stand
HL
..................
..
..
.....
..
..
27
Tips
and
hints
:
What
to
do
if
..
..
......
.
.......
..
.......
...
58
Swinging-arm
stand
ESD
...
.
.. ..
...
.
...
.....
..
.....
.....
28
Care
and
maintenance
...
...
...
.
........
.. ...
..
......
...
59
Large
swinging-arm a
nd
table-c
lamp
stands
...
.
..
.
..
......
30
Calculating
to
t
al
magnification
and
field
of
view
diameter
...
..
....
...........
.
....
..
....
....
....
61
Optics
carrier,
optical
accessories
Opt
i
cal
data
for
MS5,
MZ6
.. . . ..
..
...
..
......
.
..
...
...
...
62
Spacer
rings,
objective combinations ..
...
....
... .
..
..
...
.
32
Optical
data
for
MZ7
5,
MZ95
....
..
...
.....
...
..
....
..
....
64
Microscope carri
er
...
....
...
..
.
..
.
..
.
....
.. .
....
..
....
.
34
Optical
data
for
MZ125
,
MZ16
,
MZ16
A .. .
..
...
...
.
......
..
66
Optics
carrier
...
. .. ..
.......
.
..
.
........
.
......
.
...
.
..
.
34
Dimensions
..
.
..
.
.. ..
.
.............
..
.
.......
...
..
.....
67
Binocular t
ubes
, optical accessories
.....
...
..
.
....
...
....
35
Objective
no
s
epie
ce
(MZ16
and
MZ16
A)
......
..
...
.
..
....
36
4
Le
i
ca
M
Ste
r
eomicroscope
sUs
er
Manu
al
Leica
M
Ste
r
eomicro
s
copes
User
Manual
5

6
General directions Read
the
user
manual
and
safety
instructions
before
starting
up
.
Permitted uses
The
Leica MS5,
MZ6,
MZ7
s, MZ9
s,
MZ12
s.
MZ16
and MZ16 A stereomicro-
scopes are optical instruments which
use
magnification for improved
visual
iza
ti
on
of objects, their details
and
specimens from technology and
natural sciences.
The
basic outfit, which includes a stand and a light
source, can
be
supplemented w
it
h vario
us
modules for photography,
TV
,
dual station viewing
and
other applications.
Prohibited • The use of the instrument in a different manner from
that
de
sc
ribed
in
uses this user manual can lead to injury, malfunction
and
damage.
• The Leica MS5, MZ6, MZ7s, MZ9s,
MZ12
s,
MZ16
and
MZ16 A stereo-
microscopes must not
be
used for
eye
examinations
and
operations.
•
Do
not fit different plugs.
Do
not dismantle optical systems or mechani-
cal parts unless instructions for doing
so
are given
in
the user manual.
Place • The Leica
MS5
,
MZ6,
MZ7s, MZ9s, MZ12
s.
MZ16
and
MZ16 A stereo-
of use microscopes are intended
ma
inly for indoor
use
.
•
If
the stereomicroscope
is
used outdoors, protect
it
from dirt
and
mois-
ture. Lamps
and
electrically-operated stands from Leica may not
be
used outdoors.
Use in
ESD
The
optics carriers
MS5
, MZ6, MZ7s, MZ9s, MZ12s, MZ16
and
MZ16
A,
the
protected rooms binocular tube, Ergo
Tube™,
ErgoWedge™ 5
°-
25°,
the swinging-arm
stand
ESD
and
the cold light sources Leica
L2
and
CLS
consist of
ESD-
discharging material (surface resistance <
10
" ohms/squared, discharge
t1me
<2 seconds,
1,000
Vto
100
V).
Servicing
Responsibilities
of person in charge
of instrument
- A connection socket for a 0 4
mm
grounding cable
is
provided
on
the
microscope carrier.
-There are
two
connection sockets for 0 4
mm
grounding cables
in
the
base of the swinging-arm stand.
Repairs may
on
ly
be
carried out by Leica-trained service technicians.
Only original Leica spare parts may
be
used.
• Ensure that personnel who use the instrument have read
and
under-
stood this user manual and in particular the safety instructi
ons.
• Ensure
thatthe
Leica MS5, MZ6,
MZ7
s, MZ9s,
MZ12s
, MZ16
and
MZ16
A
stereomicroscopes are operated, maintained
and
repaired
on
ly
by
authorized
and
trained personnel.
Leica
M Stereomicroscopes -
Safety
concept
Safety
rf'g;ulations
Workplaces with stereomicroscopes facilitate
and
improve the
viewing task, but they also impose high demands
on
the eyes
and holding muscles of the user. Dependent upon the duration
of uninterrupted activity, this may lead to asthenic trouble
and
muscular-skeletal pain
so
that
suitable measures must
be
taken
to reduce the strain:
-perfect design of workplace (see p.
14).
work content and
work process (frequently changing activities).
-detailed introduction ofthe personnel while considering
ergonomic
and
organizational work-related aspects.
The
ergonomic optics concept
and
the design
ofthe
Leica M
series
aim
at limiting the strain
on
the user to the lowest possi-
ble level.
Direct contact with eyepieces can
be
a potential transmission
path for bacterial
and
viral infections of the
eye.
Users should
be
made aware ofthe potential risk of infection.
The
risk can
be
kept at a minimum
by
using individ
ua
l eyepieces or attachable
eyecups (see
p.16)
.
Electrically-operated
items
The safety regulations listed below apply to the following items:
-Transmitted-light stand, bright field,
20
W (page
24)
-Supply (line) lamp
25
W (page
47)
-Regulating transformer 0- 7
V/40
W (page
38)
-Step transformer 4/
5/6
V (page
39)
-
Motor
focus (separate instructions)
-Motori
zed
Leica
MZ16
A
Liquids
Be
careful when handling liquids.
If spilt
on
the equipment, they can:
-cause the stereomicroscopes
and
accessories to become
electrically live, endangering personnel,
-cause damage to the equipment.
Power cables Inspect cables regularly for damage. Defective cables can:
-directly endanger personnel,
-cause the stereomicroscopes
and
accessories to become
electrically live, endangering personnel.
Leica
M Stereomicroscopes -
Safety
regulations 7

Cable
routing
Opening
the
instrument
Supply
voltage
Connections
Route
cables
with
care.
Make
sure
that
personnel
do
not
get
entangled
with
them
.
The
instrument
could
fall
over,
fall
down,
become
damaged,
damage
other
equipment,
or
cause
personal
m
Jury
.
Only
authorized
Leica
personnel
are
permitted
to
repair electri-
cal
equipment.
Before
opening
up
the
equipment,
pu
ll
out
the
power
cable.
If
you
touch
the
live
interior
of
the
equipment
,
you
may
receive
an
electric
shock.
Ensure
that
the
vo
l
tage
setting
on
the
equipment
is
correct.
A
wrong
setting
can
cause
damage
to
the
equipment.
When
connecting
power-consuming
devices
to
the
transform-
ers,
do
not
exceed
the
maximum
permitted
loading,
as
this
can
cause
damage
to
the
equipment.
Changing
bulbs
•
Before
changing
a
bulb,
disconnectthe
power
cable.
8
Integration
in
third-party
products
legal
requirements
Disposal
Conformity
with
European
Community
directive
•
Never
attempt
to
touch
the
inside
of
a
lamp
housing
or
transmitted-light
stand
while
the
instrument
is
connected
to
the
power
supply
.
•
Before
changing
a
bulb,
wait until it
is
cool,
otherwise
you
may
burn
your
fingers.
The
following
must
be
observed
if
Leica
products
are
build
into
third-
party
products:
The
manufacturer
of
the
complete
system
or
the
person
putting
it
on
the
market
is
responsible
for
adhering
to
applicable
safety
regulations,
laws
and
guidelines
.
Adhere
to
general
and
local
regulations
relating
to
accident
prevention
and
environmental
protection.
The
products
described
here
must
be
disposed
off
in
accor-
dance
with applicable local
laws
and
regulations.
The
electrically-
operated
accessories
for
the
Leica
MS5,
MZ6,
MZ7
s,
MZ9
s,
MZ12
s,
MZ16
and
MZ16
A stereomicroscopes
are
constructed
in
accordance with
the
latest technologies
and
provided
with a
statement
of
conformity with
EC
requirements.
Leica M Stereomicroscope
s-
Safety
re
gu
lations
Symbols
used
in
this
user
manual
Safety
instructions
This
symbol
denotes
information which
must
be
read
and
obeyed.
Non-observance
-
can
put
personnel
at
risk!
-
can
cause
malfunctioning
or
damage
to
the
equipment.
Warning
about
accessible
hot
places,
e.g.
bulbs.
Failure
to
respect
it
can
cause
burns!
Important
information
This
symbol
denotes
additional information
or
explanations
which
promote
understanding
.
Action
..,..
This
symbol
within
the
text indicates that certain
operations
must
be
carried
out.
Explanatory
notes
•
This
symbol
within
the
text
stands
for additional information
and
explanations
.
Leica M Stereomicroscopes - Safety regulations 9

Overview
Stereomicroscope
Magnification
changer
2
Engaging
ratchet
positions
3a
Focusing
drive
3b
Coarse
and
fine
focusing
Larger
knob:
Coarse
focusin
g,
Smaller
knob:
Fine
focusi
ng.
3c
Adjustable
ring
Changes
the
ease
of
movement
of
the
coarse/fine
drive.
4
Clamping
screw
Holds
the
optics carrier
in
the
microscope
carrier.
5
Clamping
screw
Holds
the
binocular
tube
(
or
accessory)
to
the optics carrier.
6
Interchangeable
objective
Achromatic
,
planachromatic
or
planapochromatic
Leica
MS5
Binocular
tube
7
Adjustable
eyepiece
tubes
Interpupillary distance
adju
stab
lef
ro
m
52
mm
-76
mm.
8
Wide-field
eyepieces
for
persons
wearing
glasses
Diopter
setting
adjustable wi
th
in
the
range
fr
om
+5
to
-5,
and
eye
cups
adjustable.
9
Clamping
screws
These
hold
the
eyepieces
in
the
binocular
tube.
10
ErgoTube™
The
viewing
angle
is
adjustable within the
r
ange
10
°-
50
°.
11
Built-in
double-iris
diaphragm
(MZ16/MZ16
A
only)
•
The
special
functions
of
the
Leica
MZ16
A
are
shown
in
the
user
manual
M2-116-0.
Leica
MZ6
10
Leica
M
Stereo
m
ic
r
oscopes
-
Overv
i
ew
Leica
MZ7s
Leica
MZ9s
Leica
de
sign
by
Erne
st
lgi/Christophe Apotheloz
Leica
MZ12s
Leica
MZ16
Le
i
ca
M Stereomicroscopes -
Overview
11

Use
12
Changing
the
magnification
Ratchet
steps
Magnification
changer
•
Zoom
means
continuous
change
of
magnification.
Ratchets
can
be
engaged
for certain magnifications
(see
below).
•
For
zooming
with
MZ16
A,
see
the
user
manual
M2-116-0.
MS5:
5
steps
MZ6
:6:1
zoom
MZ7
s:
7.9:1
zoom
MZ9
s: 9.
5:1
zoom
MZ12
s:
12
.5:1
zoom
MZ16
and
MZ16
A:
16:1
zoom
•
The
mag
ni
fication
changer
can
be
operated
from
either
the
right
or
the
left
side
.
~Look
into
the
eyepieces.
~Focus
on
the
object (
page
16).
~Turn
the magnification
changer
to
the
desired
position
.
MZ6:
0.8,
1,
1.25
, 1.
6,
2, 2.
5,
3.2
MZ7
s:
0.
8,
1,
1.25,
1.6,
2,
2.
5,
3.
2,
4
MZ9
s:
0.8,
1, 1.
25,
1.6, 2, 2.5, 3.
2,
4,
5
MZ12
s:
1,
1.25
,
1.6
,
2,
2.5,3.2,
4,
5,
6.3, B
MZ16
:0.
8,
1,
1.
25
, 1.
6,
2,2.
5,
3.
2,
4,
5,
6.
3,
B,
10
•
Engage
the
ratchets whenever magnifications
need
to
be
exactly reproducible.
~For
MZ6
,
MZ9
s,
MZ12
s
and
MZ16:
Press
the
switch.
~For
MZ7
s:
Turn
the knurled
ring
counterclockwise.
For
setting ratchets
at
the
MZ16
A,
see
the
use
r
manual
M2-116
-
0.
Lei
ca MStereomicroscopes -
Oper
ation
Scale
of
magnifications
Magnifications
and
fields
of
view
Magnification
with
MZ12s/MZ16
planachromatic
and
planapochromatic
objectives
MS5,
MZ6:
Display
of
magnification factors
from
0.
63-4
.
MZ7s:
Display
of
magnification factors
from
0.
63-5
.
Corresponding
scales
for other objective
and
eyepiece
combi-
nations
can
be
affixed
to
the
rotary
knobs
(page
34).
MZ9s:
Display
of
magnification factors
from
0.
63-6.
MZ12s:
Display
of
magnification factors
from
0.8-
10
.
MZ16,
MZ16
A:
Display
of
magnification factors
from
0.
71-11
.5
MZ16
A:
For
the
digital display
of
the magnification
see
the
user
manual
M2-116-0
.
The
tables
on
pages
62
-
66
provide information
about
the
magnifications
and
field of view
diameters
as
afunction
of
the
magnification
changer
position
of
the
eyepiece-objective
combination
used
.
When
the
MZ12
s/
MZ16
planachromatic
and
planapochromatic
objectives
are
used
on
the
Leica
MS5,
MZ6,
MZ7
s
and
MZ9
s,
the
magnification
is
increased
by
the
factor
1.25
x.
This
factor
is
taken
into
account
in
the
tables.
Magnification
on
Magnification
on
MZ12
s
/MZ16
MS5/MZ6/MZ7
s/
MZ9
s
1x
plan/planapo
1.
25
x
1.6x
planapo
2x
0.
63
x
planapo
O.Bx
0.5
x
plan
0.
63
x
0.8x
plan
1x
2x
planapo
2.5x
Leica
MStereomicroscopes -Operation
13

14
Preconditions
fm
working
comfortably
Ergonomics,
viewing
height
•
Pay
great attention when setting
up
you
r stereomicroscope.
You
wi
ll
only benefit fully from the outstanding
op
tical
and
ergonomic advantages of the instrument if
you
set it
up
precisely
in
accordance with
the
directions already
given.
•
For
fatigue-free work,
use
the ErgoModule™.
• Arrange your workplace
as
well
as
possible,
and
pay
attention
to
the heights of chair
and
table.
• When sitting,
use
the entire seat
area
and
also
the
back
support of the chair.
• Support your forearms.
• During work
breaks,
stand
up
and
move
arou
ndfor exercise.
Please
also
observe
the
note
on
p.
7.
Ergonomics
The
reasons why
users
often cannot work comfortably with
the
45
° tube
are
the differing heights
of
the outfits, the various
accessories
and
working distances,
and
the fact that
the
same
ins
trument
is
often
used
by
several
people
of
differing
builds.
This
problem
can
be
effectively
solved
by
using
the
Leica
ErgoModule™.
Viewing
height,
viewing
angle
If
you
have
problems with
the
viewing configuration
on
your
stereomicroscope,
ask
your
Leica
consultant forthe most
ergonomic
Leica
so
lutions.
The
viewing height
is
correct when your
head
and
back
are
comfortably positioned.
•
On
the
Ergo
Tube™,
the viewing
angle
is
adjustable from
10
°-
50
°.
lliJ>
Holding
the
eyep
i
ece
tubes
at
their
bases,
swi
ng
them
up
or
down
(see
arrow).
.
.,
,
,
~0
'
"
~
10°
Lei
ca M
Ste
r
eomi
crosco
pes
-
Op
era
tion
ErgoModule™
-
ErgoWedge™
5° -
25
°
-
ErgoWedge™
±
15
°
-ErgoModule™
50
mm
-
The
Ergo
Tube™
45°
has
eyepiece tubes wh
ich
are
65
mm
longer than the standard
ones.
Magnification factor 1.6
x.
MZ16
A:
The
magnificati
on
factor
can
be
toggled
on
and
off
using
the
OPT
key.
-ErgoModule™
30
-
120
mm
Relubrication
may
be
required
after several years (please
send
the
item
to
Le
i
ca
Service).
Ergo
objective
The
achromatic
Ergo
objective
0.4
x-0.63x
can
be
used
to
ergonomical
ly
and
accurately focus
in
the
range
of
90
mm
(63
.
5-153
.5
mm)
without changing
the
viewing height. At the
same
time, magnification
and
working distance
can
be
changed without time-consuming change of objectives.
Motor
focus
Frequently repeated fine-motor tasks,
such
as
focusing
by
hand
,
can
lead
to
muscle
fatigue
and
hand
problems.
Us
i
ng
the
Leica
motor focusing
system,
every equipment
can
be
moved
up
and
down without applying force.
Interpupillary
distance,
eye
contact
Interpupillary
distance
The
interpupillary distance
is
correct
when
you
see
asingle circular field when
viewi
ng
wi
th
both
eyes.
~
76mm
~
lliJ>
Look
into
the
eyepieces
and
use
bo
th
hands
to
move
the
eyepiece tub
es
closer
together
or
further apart.
Locating
the
exit
pupil
The
distance between
eye
and
eyepiece measures approx.
22
mm
for
10
x
/21
Bwide-field eyepiecesfor persons wea
ring
gl
a
sses
and
12
mm
for
10
x
/21
wide-fi
eld
eyepieces.
your
eyes
un
til
you
can
see
the
complete
~
im
a
ge
field without corner cutting.
lliJ>
Slowly approach
the
eyepieceswith
!I
~
~
.
"'
'f:?
"'
l
'
Yf
Leic
a M
St
ereom
icroscopes -
Oper
at
ion
15

16
Selecting
the
degree
of
eye
contact
Soft
eyecups
You
do not wear glasses
and
prefer close contact with 1Ox
/21
B
wide-field eyepieces for persons wearing glasses:
~Holding
the diopter ring,turn the
eye
cup
counterclockwise until it is released.
~Pull
the eyecup upwards.
~Ho
l
d
i
ng
the diopter ring,
turn the
eye
cup clockwi
se.
Persons wearing glasses need the
whole ofthe available distance.
Move the
eye
cups to the lowest
position.
The
1D
x/
21
Bwide-field
eye
pieces for persons wearing glasses
are supplied with soft
eye
cups, whi
ch
can
be
attached to the in-
tegrated
eye
cups made of hard plastic to achieve the
fo
llowing:
-Prevent eye infections if several users are working at the
same instrument (see
p.
7).
-Protect
yo
ur glasses from being scratched.
The
10
X/
21
wide-field eyepieces are supplied with laterally
tapered soft eyecups.
Setting
the
working
distance,
focusing
=obtaining
a
sharp
image
•
To
focus,
use
the
fo
cusing drive to
ra
i
se
or lowerthe stere-
omicroscope until the feature of interestis
in
focus = working
distance to the objective.
The working distances
ofthe
various objectives are listed
in
the tables
on
pages
62-
66
.
• The focusing drive can
be
operated from either the right
or
the left side.
• When focusmg
on
a new object, always start with the lowest
magnification, because the largerfield of view
ma
k
es
the
o
bj
ect
easier to find.Afterwards, select the desired magnifi-
cation.
Le
ica M Stereomicrosc opes -
Ope
ration
Coarse/fine
drive
Adjusting
the
free
mning
of
the
focusing
drive
Focusing
drive
MS5-
MZ9s
Focusing
drive,
coarse/fine
MS5-
MZ12s
Focusing
drive,
coarse/fine
MZ16,
MZ16A
~Place
the object beneath the
ob
jective.
~Engage
the lowest magnification
(page
12).
~Look
into the eyepieces.
~Using
the rotary knob, bring the
object into focus.
~Focus
approximately with the
larger knob.
~
Fine-focus with the smaller
(concentric) knob.
Is
the focus movementtoo loose or too tight? Does the outfit
tend to slide downwards?
You
can adjust the movement
in
accordance with the weight ofthe outfit
and
with your own
personal requirements:
~Hold
the drive knobs firmly
wit
h
both hands
and
turn them
in
opposite directions until you
obtain the required degree of
resistance during focusing.
~Turn
the black ring
on
the
right-hand drive knob until
you obtain the required degree
of resistance duri
ng
focusing.
~Hold
the left fine drive.
~Turn
the right fine drive until the desired resistance during
focusing
is
reached.
Leica M Stereomicroscopes -Operation
17

18
Microscope carrier
for
stereoscopic
observation
Stereoscopic
observation
Microscope carrier
•
This
microscope
ca
rrier permits
only
stereoscopic observation, using
both
beam
paths.
•
The
microscope carrier
can
be
fi
tted
to the drive
housing in either of two
posi
tions
(see
page
34)
:
•
The
optics carri
er
can
be
turned sideways
in
the microscope carrier ift
he
user
needs
to
work from the si
de:
..,.
Re
l
ease
the clamping screw.
..,.
Turn
the
optics carri
er
sideways
as
required.
..,.
Retighten
the
cl
amp
i
ng
screw.
Microscope carrier
AX
The
stereomicroscopes
can
be
equipped with t
he
microscope
carrier
AX
f
or
stereoscopic
and
axial
observation instead of
the standard
mic
roscope carrier
(page
34)
.
Different
mode
ls
are
avai
l
able
for
MS5,
MZ6,
MZ7
s,
MZ9
s
and
for
MZ12s,
MZ16
and
MZ16
A.
The
achromatic objectives
and
the planochromatic
lx
fit
in
the
th
r
ead
in
the
AX
microscope
carrier for
MS5
,
MZ6,
MZ7s,
MZ9s
(p.
33).
The
planochromatic
and
planapochromatic objectives fit
in
the
thread
in
the
AX
microscope carrier for
MZ12s,
MZ
1
6,
MZ16
A
(p.
33).
In
addition, the
AX
microscope
ca
rri
er
features a clamp-
ing
screw
fo
rfixing it
in
one
of
3 positions
and
an
aperture which
can
be
turned
manua
l
ly
(see
below).
Otherwise,
the
use
of
and
switchi
ng
to
the
left,
middle
and
right position
are
identical.
•
The
opti
cs
carrier
cannot
be
turned sideways
in
the
micro-
scope carrier
AX
.
A three-dimensional
image
is
required for
examining
plastic
objects
and
for working.
..,.
Shift the stereomicroscope to the central positi
on
.
Le1c
a M Stereomicroscopes -
Ope
ration
Axial
observation
Aperture
The
parallax-free i
mag
i
ng
produces
more
prec
i
se
results
in
photogr
aphy,
measuring
and
polarization.
..,.
Shift the stereomicroscope to the left
or the right positi
on.
The
object
is
now
located directly beneath either
the
left
or
the
right
beam
path
.
• When photographing or measuring,
se-
lectthe
beam
path
above
which
the
pho-
totube
or
measuring graticule
is
located.
A rotating aperture is
build
into the
AX
microscope carri
er.
lt
prevents interfering reflecti
ons
during
axial
observation with
coaxial incident illuminator
and
quarter-wave plate.
• With the
AX
microscope carrier for
MS5,
MZ6,
MZ7s, MZ9s,
the aperture automatically turns during
the
moving
process.
• With
the
AX
microscope carrier for
MZ12s,
MZ16,
MZ16
A,
the
aperture
is
located underneath
the
microscope carrier
and
must manually beturned
as
follows:
Please
note:
-
In
the position for stereoscopic observa-
ti
on
through
both
beam
paths, turn
the
aperture counterclockwise until it
stops.
Otherwise
the
field
of
view
is
unde
r
ex-
posed
(vignetting).
-
In
the
pos
ition for
axial
observation
through
the
right or left
beam
path, turn
the
aperture clockwise until it
stops.
Leica M Stereomicroscopes -IJperation
19

20
Tips
for
your
work
[I]
A Preparations
Adjusting dioptersettings and parfocali1y
•
The
dioptr
ic
range
is
from
+5
to
-5.
The
fo
ll
owing
adjus
t
ments
must
be
carried
out
on
ly
once
by
each
user.
The
procedures for adjusting
the
diop
t
ric
setting
and
the
parfocality
are
somewhat differ
ent
if
an
eyepiece
conta
i
ns
agraticule.
Refer
to
the
user
manuals
for
the
MPS
modular
photomicrographic
systems
or
for
measuring.
~Install
the
lamp
.
~Set
the
interpupi
ll
ary distance
and,
if
necessary,
the
viewing
height.
~On
the
video/phototube:
Move
the
l
ever
to
position
"
VIS
"
and
open
the
diaphragm.
~Set
the
double
-iris di
aphragm
to
"OPEN."
~Adjust
the
eyecups.
~Set
both
eyepieces
to
zero.
~Set
the
microscope carrier
AX
for
stereoscopic
observation.
B
Focus
on
the
1.
Place
aflat test object
under
the
test object objecti
ve.
2.
Engage
the
lowest magnification
position.
~Use
the
coarse
focusing
drive
to
set
the
working di
stance
(page
16)
.
3.
Look
i
nto
the
eyepieces
~Use
the
foc
using
drive
,
to
bring
the test object
in
to
foc
us.
leica
M Stereomicrosco
pes
-
Op
er
at
ion
C Adjusting
dioptric settings
D Inspecting
parfocality
4.
Engage
the
highest
magnification
position.
5.
Optimize
the
focus
using
the
focusing
drive.
6.
Engage
the
lowest magnification
positi
on.
7.
Do
not
look
into
the
eyepieces.
~Rotate
the
eyepieces
counterclockwise
in
the"+" direction
as
far
as
the
stop
(+5
diopters).
8.
Look
into
the
eyepieces
~
Slowly
rotate
each
eyepiece
individually
in
the"
-"
direction until
each
eye
sees
the
ob
ject sharply
imaged.
9.
Engage
the
highest
magnification
position.
10.
Refocus
if necessary.
11.
Slowly
move
t
he
magnification
changer
from
the
lowest magnification
to
the
highest.
•
The
image
sharpness
must
remain
constant (parfocal). Otherwise,
repeat
the
procedure.
Leica M St
ereom
i
croscopes-
Operation
21

Assentbly
22
The
components
1a
Inc
ident-light
base
wi
th
ste~ge
pla
te
1b
Tra
n
sm
itted-light
base
with g
lass
stage
ple~
t
e
2a
Side-
f
aced
column
with dri
ve
h
ous
i
ng
,coarse/fine
2b
Side-
f
aced
column
with
dr
i
ve
hous
i
ng
,
300
mm
or
500
mm
3 Microscope carrier
4
Op
tics
ca
rr
ier
5
Inte
r
ch
angeable
objective
6
Binocula
rtube
7
8 Wide-fieldey
ep
i
ece
for spectaclewearers,with
eyecups
Various
ErgoM
o
dule™
or accessories for
video,
photography,
drawi
ng,
fluorescence, coaxial incident li
ght
etc.
Le
1ca
M Stereomicroscopes-Assembly
Side-faced
column-+
incident-1
ight
base
Side-faced
column
-+
transmitted-light
base
Stand
Incident-light
stan
d
~
Remove
the
st
age
pla
t
e.
~
Insert 3
Al
i
en
screws
into
theund
erside
of
th
e
baseplate
and
secure th
em
to
the
bottom
end
of
the
side
-faced
co
lu
mn
.
~Insert
the
stage
pl
ate.
Tofit
the
microscope carrier and
the
ste
reomicroscope,
see
page
34
Transmitted-light
stand
~
Pu
ll
the
power
cable
ou
t
of
the
socket
in
the
stand.
~
Remove
the
gl
ass
stage
pla
t
e.
~
Carefully invert
the
transmitte
d-
light
base
and
remove
7
Alien
screws f
ro
mt
he
base.
~
Lift
off
the
lower
par
t ofthe
base
.
~
Reve
r
se
the
u
pper
part of
the
base
.
~
Insert 3
Alien
sc
r
ews
into
the
cover
pl
ate
from
beneath
and
secu
re
th
em
to
the
bottom
end
of t
he
si
d
e-
faced
column
.
~Replace
and
secure
the
base.
~
Insert
the
glass
stage
plate.
~F
i
t
t
he
microscope carrier
and
the
stereomicroscope
as
descri
bed
on
page
34
.
Le
i
ca
M Stereomicroscop
es
-Assembly
23

Fitting
the
bulb
Filters
Starting
up
24
Transmitted-light
stand,
brightiield,
20
W
&,
Follow
the
safety
instructions
given
on
page
7.
~Fit
the side-faced
col~mn
to the transmitted-l
ig
ht base
as
described
on
pa
ge
23
.
&
Never
touch
the
bulb
when
it
is
hot.
Y . b
ou
may
sustam
urns.
•
Do
nottouch
the
new
6
V/20
W halogen bulb directly
with
your fingers, because finger marks can cause it to crack
when heated.
Remove finger marks immediately with a cloth dipped
in
pure
alcohol.
~
Insertthe slider together
with
the
bulb socket.
~
Hold the new bulb in a cloth
an
d push
it into position.
~Push
the slider
caref~lly
but complete-
ly back along t
he
ra
il to the stop.
•
The
transmitted-light stand contai
ns
a
KG1
filter.
•
Yo~
can insert other 0
50
mm
filters if you need them:
~
Pull out the slider.
~
Insert the 0
50
mm
filter.
~
Push
the slider
caref~lly
but
completely back along t
he
rail to the stop.
The
ex-factory setting of the voltage
se
lector
is
230
!for volt-
ages between
200-240
V)
and
t
he
fuse holder contains
two
160
mAT
fuses.
For
100
-
120
V power supply:
~Se
t
the voltage
se
lec
tor
to
115
.
~
Fit the enclosed two
315
mAt fuses
in the f
us
e holder instead
ofthe
two
160
mAT
fuses !see the section
"Changing fuses" o
n,
the right).
~
Conn
ect the power cable tothe socket
ofthe
base
and
to
t
he
power source.
Lei
ca M
St€r
eomicro
sc
op
es
- A
ss
embl
y
Changing
fuses
Transmitted
or
incident
light
Switching
on
the
transformer
Transmitted
light
(bright
field),
or
in-
clined
transmitted
light
Transmitted
light,
bright
field
Inclined
transmitted
light
Use
:
-Two
160
mAT
fuses for voltages between
200
-
240
V.
-Two
315
mAT
fu
s
es
for voltages between
100
-
120
V
!see the section "Starting
up
"
on
the left).
~
-,11~~'
~
~~-·
~Disconnect
the power cable from the stand.
~
~
Pull out the
f~se
holder.
~
Fuse
at rear: Press
o~t
downwards.
~Spare
fuse at
front
Pull out sideways
and
insert into holder at rear.
~
P~sh
the fuse holder back into
po
sition.
• Connect
an
incident lamp to the transmitted-light stand,
if required.
• Either transmitted light or incident light can
be
switched
on.
~
For
transmitted
light
Switch
in
position
I.
~
For
incident
light
Switch
in
position
11.
~
Use thumb wheel to
adjust brightness.
~On
the rear of the stand: Use the thumb
wheel to switch
on
the transformer
and
to adjustthe brightness.
~
Switch
on
transmitted light.
Bright field
is
s~
i
table
for examining translucent objects featur-
ing contrasting structures. The object
is
directly illuminated
from be
low
and
is seen
in
its natural colors against a bright
backgro~nd.
~
For
transmitted light, bright field:
Pu
ll lever forwar
ds.
Inclined
tr
a
nsm
itted light
whi
chtravers
es
the object obliquely
will
p
rod~
c e
effects advantageous for observi
ng
small translu-
cent objects such
as
foraminifera
and
fish eg
gs
.
~
Move the lever slowly
towards
the col
um
n of the stand until
the desired effect
1s
obtaine
d.
Lei
ca
VI
St
ere
omi
cros
co
pes
-
Asse
mb
ly 25

26
Transmitted-light stand, bright
and
dark field
The illumin
at
i
on
of
the transmitted-light stand bright
and
dark
field consists of a fi
be
r-optic light guide and a light source.
.1.\.
Observe the safety regulations from the
~
manufacturer ofthe
light
source.
..,.
Unplug the fiber-optic light guide f
rom
t
he
con11ect
i
on
in
the stand.
..,.
Fit the side-faced column to the transmitted-light base
as
described
on
page
23
.
..,.
Fit the microscope carrier and stereomicroscope
as
described
on
page
34
.
..,.
Attach t
he
fiber-optic light guide att
he
stand
and
t
he
light source.
Starting
up
Before
us
i
ng
the light source, read the corresponding user
manual.
Transmitted light.
bright
field
Transmitted light,
dark
field
Bright field is suitable for examining translucent objects featur-
ing contrasting structures. The object
is
di
r
ec
tly illuminated
from
be
l
ow
and
is
see
n in its natural colors against a bright
background.
..,.
Slide handle to the right until the
stop: bright field.
Dark field offers
mo
re information for objects poor
in
contrast
with rather poorly marked or very fine structures.
In
this case,
the light beams penetrate the object at a flat angle.
Fine
detail
structures
and
contours contrast brilliantly
and
brightly from
dark background.
..,.
Slide the handle out to the right:
dark fiel
d.
Leica M Stereomicroscopes -Assembly
Transmitted-light stand
HL
Column-+ Base
The
column seat contains Alien screws.
..,.
Remove the glass stage plate.
..,.
Invertthe transmitted-light base
and
place it
on
a table.
..,.
Hold column against the column seat from underneath.
..,.
Insert the Alien screws into the
co
l
umn
from the top
and
tighten them.
..,.
Invert the transmitted-light base
and
insertthe glass
stage plate.
..,.
Fit
additional components such
as
the microscope carrier
and optics carrier (page
34)
.
Illumination Add a very luminous cold light source
and
a light guide with
Accessory condenser
-+Basis
Use
Tilted mirror
a channel to the transmitted-light stand
(TVC,
active 0 9
mm).
..,.
Insert light guide into the base at the rear.
..,.
Connect light guide to the light source.
For
stereomicroscopes with high apertures such
as
Leica
MZ12s,
MZ16
and
MZ16
A
wit
h objective
1.6
x, the resoluti
on
can
be
increased
by
inserting
an
accessory condenser.
..,.
Push the accessory condenser
in
to the l
ens
holder
at
the
underside of the transmitted-light base
and
secure it
with
2Alien screws.
..,.
Turn
on
the light source according to the manufacturer's
user manual
and
adjustthe light intensity.
..,.
Place a transparent object
on
the glass stage plate
and
focus.
The built-in mirror features a clear
and
an
obscured side and
can
be
rotated
and
moved.
The
obscured side provides a dif-
fused illumination.The angle of
in
cidence
in
the specimen
plane changes depending on the position of the mirror
so
that
the transmitted light bright field
via
oblique illumination can also
generate
an
illuminati
on
similar to dark field.
..,.
Rotate
and
move the mirror with t
he
black rota
ry
kn
ob on
the
right si
de
.
..,.
Adjust exact bright field
and
maximum illumination: move
mi
rrorto the stand column until it stops.
Rotate the mirror until it engages inthe
45
° position.
Le
ica
1\1
Ster
eo
microscopes - As
se
mbly
27

Assembly of
column~
base
Safety ring
~
column
Cross-member
~
column
Horizontal arm
~
cross member
Drive housing
~
horizontal arm
Swinging-arm stand
ESD
The swinging-arm sta
nd
ESO
is of conducting material [s
urf<Jc
e
r
es
istance
<10
11
Ohm
/squared, dischargetime <2 secon
ds
from
1,
000
V
to
100
V)
.Two sockets for connecti
ng
0 4
mm
ground
cables are provided
on
the base.
~
Inse
rt
4Alien screws into the
base
fr
om
beneath
and
securethem to
the
end
of
the column.
,&..
The safety ring secures the instrument and must
always
•
~:!;:;:~;~~~;~:·
~.
"""~ornbO<
tl
~
Retighten the clamping screw.
~
~
Lower the cross-member onto the
safety ring.
~
Retighten the clamping
screw
.
~
Slide the horizontal arm into the
cross-member with the connecting
piece towards you.
~
Retighten the clamping
screw
.
~
~
• The drive housing I microscope carrier can
be
fitted to
the horizontal arm in either of two positions (see page
34).
• The circular port in the microscope
carrier accepts
an
illuminator.
~
Insert the
pe
g of the
in
clinable focusing
drive into the connecting piece.
~
Retighten the clamping screw.
Optics
carrier-+
~
Fitthe optics carrier in the Q
28
microscope carrier microscope carrier.
~Turn
the optics carrier sideways
in
the microscope carrier
as
requir
ed
.
~
Retighten the clamping screw.
Le
ica M Stereomicros
cop
es
- Assemblv
l ' '
Focusing
Tilting
Altering the
working
distance
Sideways movement
Altering the overhang
The
focusing knobs are located
on
the inclinable drive housing.
Is the focus movement too loose or too tight? Does the outfit
tend to slide downwards?
You
can adjustthe movement in
accordance with the
weight
ofthe outfit
and
with your own
personal requirements:
~
Hold the drive knobs firmly with both
hands
and
turn them
in
opposite direc-
tions until you obtain the required degree
of resistance during focusing.
Possible adjustments
~
Release the clamping screw.
~
Tilt the instrument.
~
Retighten the clamping
screw
.
~
Release the clamping
screw
.
~
Raise or lowerthe cross-member
as
re-
quired together with the horizontal arm.
~
Retighten the clamping
screw
.
Reposition the safety ring (see page
28).
~
Release the clamping
screw
.
~
Move the instrument sideways
on
the crass-member.
~
Retighten the clamping
screw
.
~
Release the clamping screw.
~
Displace the horizontal arm.
~
Retighten the clamping
screw
.
Le
i
ca
M Stereomicros
co
pes -Ass
emb
ly
29

30
Large
swinging-arm
and
table-clamp
stands
Table-clamp
stand
11>-
Use
the
clamp
to
secure the
coiLJmll
to
atable
top
20
mm
-
50
mm
thick.
Swinging-arm
stand
11>-
Insert 4Alien screws i
nto
the
base
f
rom
Safety
ring
-+
co
l
umn
Safety
ring
-+
column
limitation
Drive
hous
i
ng
-+
horizontal
arm
beneath
and
secure t
hem
to
the
end
oi
the column.
.I.\
The
safety
r
ing
secures
the
instrument
and
must
always
~
be
positioned
beneath
the
horizontal
arm.
11>-
Position the safety
ri
ng
with the screw
head
u
ppermos
t
and
towards
you,
and
slide it down the
column
.
11>-
Re
tighten
the
clamping
screw.
11>-
lower
the horizontal
arm
onto
the safety
ri
ng
, with
its
end
poi11ting
towards
you.
11>-
Ret
ighten the
clampi11g
screw.
The
screw
head
must
engage
the
notch
.
lt
limits
the
lateral
movement.
lt
may
not
be
removed,
and
neither
may
the
safety
ring
be
milled
down.
If
the
horizontal
arm
is
swung
out
beyond
the
limit,
the
instrument
may
fall
over
.
Th
is
section describes the
assembly
of
the
focusing drive with
side-faced carrier
rod
.
To
fitthe focusing drive with
peg
, refer
to
page
28
.
11>-
Re
lease
the
clamping
leve
r.
11>-
Tilt
the
carrier
rod
downwards.
11>-
Retighten
the
clamping lever.
11>-
Slide
the
dri
ve
housing
with side-fa
ced
carrier
rod
on
to
the
horizontal
arm.
11>-
Ret
igh
ten
the
clamping
lever.
The
clamping levers
can
be
posi
t
ioned
as
required.
11>-
Pu
ll outthe c
lamp
ing
lever a
long
its
axis
and
turn
it.
Leica
1\11
Ste"eomicroscopes - Assembly
Microscope carrier-+
dr
i
ve
housing
Optics
carrier
-+
microscope
carrier
Tilting
Focusing
with
coarse/fine
drive
Altering
the
working
distance
Sideways
movement
11>-
Secure
as
des
c
rib
ed
on
page
34.
11>-
Secure
as
described
on
page
34
.
Possible
adjustments
11>-
Release
t
he
clamping
lever.
11>-
Tilt the instrument.
11>-
Retighten the
cl
a
mping
lever.
11>-
Set
large distances with the larger
knob.
11>-
Fine-focus with the smaller
~concentricl
knob.
11>-
Using
the
ring
on
the
right-hand drive
knob,
adjust
the
ea
se
of
movement
of
the d
ri
ve.
11>-
Re
lease
the
clamping screw.
11>-
Raise
or
lowerthe
horizontal
arm
.
11>-
Retighten
the
clamping screw.
Reposition
the
safety
ring
~see
page
301.
11>-
Re
l
ease
the
clamping screw.
11>-
Pu
ll out
or
push
in
the
horizontal
arm
.
11>-
Retighten
the
clamping screw.
During
the
sideways
movement,
observe
the
limit
stops
tsee
page
301.
Altering
the
overhang
11>-
Re
lea
se
the
clamping screw.
11>-
Di
splace
the
horizontal
arm.
11>-
Re
tighten
the
clamping screw.
Leica M Ste
reo
mi
c
ro
scopes - Ass
em
bly 31

Optics carrier, optical
accessories
Spacer ri
ngs
Sp
ace
r rings
are
required in order
to
attach
the
obje
ctives l
or
cert
ai
n instr
ument
con
ligurations.
• The L
eica
MZ95
is
supp
liedwith a
sp
a
cer
ring
(1
0
446
393
)
f
or
the
smalle
r-dia
meter
achromatic o
bj
ecti
ve
s and lx
p
la
n
ac
hrom
atic objective.
When
t
he
s
pace
r ri
ng
has
bee
n
re
mo
ved by
app
lyi
ng
t
he
t
oo
l
prov
ided, the l
arg
er-di
ameter
p
la
n
ach
rom
at
ic
an
d
pl
a
napoc
h
roma
tic
ob
jectives for t
he
M
Z125
/
MZ
16can
be
used
.
•
The
L
eica
MS5
,MZ6
and
M
Z7
5c
an
al
so
be
equipped
wi
th
the
lar
ge
r-d
iame
t
er
plana
chromatic
and
planap
oc
hr
omati
c
oo
j
ec
-
tives for
the
MZ
12
5/MZ
16
if asp
ac
er
ri
ng
is
fitted (
10
446
1
72
).
• W
hen
the
MZ125
/
MZ
16
planac
hroma
ti
c and
pl
anapo-
ch
rom
atic o
bj
ec
tive
s are u
se
donthe
Le
i
ca
M
S5
,
MZ6
, M
Z7
s
an
d MZ
95
,
th
em
ag
n
if
i
ca
ti
on
is incr
eased
by the
fac
tor 1
.2
5x.
·
~
~
~
104453
71
MZ7
s
10
44
6
27
2 M
l9
s
~
10445614 Ml6
10
445
613
MS
5
~
10
411
589
1x
10
422
562
1.5
x
10
422
56
1
2x
10
4
73
832
0
.8x
10
445
201
0.63x
10
422
563
0.5x
10
422
564
0.
32x
Ach
romat
10446275
1x
Pl
an
@ 103
15300
10
445
352
32
~
10445 1
72
t
~
10
44
6
157
0.5x
Pl
an
10
44
7 1
48
0
.4-0
.
63x
E
rgo
10
44
7
075
O
.Bx
Pl
an
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104
12
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ana
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an/Pianapo
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10
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35
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l
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ca MS
te
re
om
icroscopes - Assem
bly
10
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61
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0.5x Pl
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10
447 0750.
8x
Plan
10
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230
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a:
leica MStereomicr
os
copes User Manual 33

Microscope
carrier~
drive
housing
Microscope carrier
The
microscope carri
er
can
be
attached to the
fo
cus
i
ng
drive
in
2positions:
•
For
short working distances a
nd
for
fl
at
objects,
use
Pos
iti
on
1.
Use
this position
also
in
con
junction
with the attachment for vertical
ar1d
ob
l
ique
observation (
see
deta
i
led
in-
structions
I.
•
Use
Po
si
tion 2for
long
working
distances
and
for
bulky
objects.
Ill>
U
se
an
Al
i
en
screw
to
secure the micro-
scope carrier
in
the position required.
A connection
socke
t for a0 4
mm
grounding
cable
is
provided
on
the
microscope carrier.
Microscope
carrier
AX
• Different
models
are
available for
MS5
,
34
Optics
carrier~
microscope
carrier
Apply
the
adhesive
scale
of
magnifications
MZ6,
MZ7
s,
MZ9
s
and
for
MZ12
s,
MZ16
and
MZ16
A(seep.
181
.
•
The
microscope carrier
AX
can
only
be
fitted in posit
ion
1
to
the focusing drive.
Optics
carrier
Ill>
Gently
lower the
opt
i
cs
carrier
into
the
microscope carri
er.
....
Use
the screw to
secu
re
the
optics
carrier inthe
pos
ition required.
Only
for
MZ75:
....
Remove
the
sc
rews.
Ill>
Remove
the
rot
ary
k
nob
s.
....
Apply
the
adhesive scales.
....
Replace
the rotary
knobs
.
Leica M Stereomicroscopes -Assemllly
&r
l
::
1
Binocular
tube
Ill>
Release
the
clamp
i
r1g
screw.
• If
you
have
an
accessory such
as
an
ergo
module
(pa
ge
14).
a
coaxia
l illumi-
nator
(page
471
or
avideo/phototube
(page
521,
fit it now
to
the
opt
i
cs
carrier.
Tighten
the
clamping screw.
Ill>
Tilt the binocular tube
and
insert
the
edge
of
its dovetail
ring
beneath
the
two
lugs
on
the opti
cs
carrier.
....
Move
the
binocular tube
from
side
to
side
until
the
locating
sc
rew
on
the
opt
i
cs
carrier
engages
the guide.
Ill>
Retighten
the
clamping screw.
Optical
accessories
Eyepiece
....
Insert a graticule, if necessary
(page
56).
Objective
&
....
Release
t
he
clamping
screws
on
the
eye-
pieces.
Ill>
Insert
the
eyepieces
fully
into the
eye-
piece tubes.
Check
that they
have
seate
d
correctly.
Ill>
Tighten
the cl
amping
screw.
•
You
can
extend
the overall magnification
range
by
using
1D
x.
16
x,
25
x
and
40
x
wide-eyepieces for persons wearing
gl
asses.
Hold
the objectives
dur
i
ng
assembly
and
disassembly to pre-
ven
tt
hem
from
fa
lling
on
the
stage
plate.
This
applies parti
cu-
larlyto
the
2x planapochromatic
ob
jective, which
is
very
heavy.
Remove
the
specimens
from
the
stage
plate.
Ill>
Screw
on
the
se
lected objecti
ve
counter-
clockwise.
~
•
Some
in
st
rumen
t configurations r
equ
irespacer ri
ngs
in
order
to secure the objectives
(page
32)
.
These
ri
ngs
are
fitted
and
re
moved
us
i
ng
the t
ool
prov
ided
.
•
The
Leica
MZ9
sis supplied with aspacer
ri
ng
for
the
achro-
mat
ic objectives
and
for t
he
1x p
lana
ch
r
omatic
objective.
When t
he
spacer r
ing
has
been
removed
by
applying the
too
l
prov
ide
d,
th
e larger-
dia
meter planacllromatic
and
planapo-
chromatic objectives for
th
e
MZ125/MZ16
can
be
used
.
Lei
ca
M S
te
r
eom
ic
ro
sc
opes -Assembly
35

36
Preparation
Assembling
l x
planapochromatic
Objective
nosepiece
• Only for
leica
MZ16
and MZ16 A
-Hold objectivesduring assembly
and
disassembly to p
re
vent them from
falling
on
the stage plate.This applies particularlyto t
he
2x planapochro-
matic objective,
whi
ch
is
very heavy.
Remo
ve the specimensf
rom
t
he
stage plate.
-
The
ad
justable ring nutwith the 1x planapochromatic objective
is
fastened
with 2 Alien screws after the adjustment. loosen the Alien screws if you
need
to
remove the objective.
-Attach t
he
limit stop to the stand column.
lt
preventsthe 2x objecti
ve
fr
om
hitti
ng
the limit stop when the objective nosepiece
is
rotating,
if
the work-
ing distance was changed x
whi
le worki
ng
with the 1objecti
ve
.
-
Caution:
Carefully rotate the objecti
ve
nosepiece
and
take care not to
pinch your fingers between column
and
objective or objective nosepiece.
MZ16
A:When changing to objective l x or 2x, press the
OPT+
SEL
function
keys simultaneously.
The
displ
ay
shows the curr
enttotal
magnification,
and
in
REF
mode it shows the current measurement with objecti
ve
1x or 2x.
A 1or 2
in
front of the value indicates the selected objective.
Remove the securing strap from the objective nosepiece:
~
Remove both screws (at the bottoml.
Leica MZ16
and
MZ16
A are delivered with athreaded adapter for objectives.
Remove the adapter to use the objective nosepiece.
~
Invertthe optics carrier.
~Loo
sen
3 screws
and
remove the adapter.
The objective nosepiece features two threads:
- a fixed thread for the 2x planapochromatic objective
- a thread with 2 Alien screws fo rthe l x planapochromatic objective with
adjustable ring nut.
Th
e adjustable ring nut is supplied separately
and
used
to adjust the parfocality between both objectives.
Invert the objective nosepiece to simplify the assembly ofthe objectives.
~Sc
r
ew
on
the adjustable ring nut
on
the thread
ofthe
l x planapochromatic
objective until the limit stop and tighten
it
with the enclosed wrench.
~Sc
r
ew
in the 1x planapochromatic otJjective with the ring nut in clockwise
directi
on on
the side with the 2Alien screws.
• The fine-pitch thread
on
the ring nut consists of
24
threads.
You
should expect at least
40
turns.
The
ad
justable ring
nu
t must never
be
set higher than the top
su
rf
ace
of
the
nosepiece plate to prevent the nosepiece from locking while
ro
tating
leica M Stereomicroscopes - Ass
emb
lv
Assembling
2x
planapochromatic
Assembling
objective
nosepiece
Adjusting
parfocal
~
Carefully turn and ti
gh
t
en
the 2x planapoch
ro
matic objective clockwi
se
to
the limit stop
without
applying excessive force.
~
Raise
the focusing drive with the drive heads to
provide sufficient space for the 2x planapoch
ro
-
matic objective.
~F
it
the objective nosepiece like a microscope car-
rier
wit
hthe Alien
screw
(see page
34
, position 1
).
~
Insertthe optics carrier in the objecti
ve
nosepiece.
~
Retighten the clamping
screw
~Att
ach
the limit stop to the column of the st
and
so
thatthe working distance beneath the 2x objective
will always measure approx.
15
mm.
I
1i
• Fit
and
adjust the l x
and
2x planapochromatic objectives
as
described.
The
image then remains parfocal in the range of < 0.
05
mm,
and
refocusing
after changing the otJjective
is
no
longer requir
ed
.
•
Use
a very fine, flat otJject
(e
.
g.
an
object micrometer) for the adjustment,
which allows maximum focusing even at highest resolution.
~Scr ew
the 2x planapochromatic objective into the beam path.
~Ad
justing
the diopter settings
and
parfocality (seep.
20)
.
~Screw
the 1x planapochromatic objective into the
beam path.
~
Observe the same object with smallest magnifica-
tion without changing the settings made with the
2x planapochromatic objective.
~Turn
the 1x planapochromatic
ob
jective counter-
clockwise until the object can
be
seen in focus
atthe smallest magnification.
~Sl
owly
zoom
to the highest magnification. At
the same time correctthe focus
by
turni
ng
the
1x planapochromatic objective.
• Verify thatthe object
is
in
focus at highest magnification with t
he
2x planapochromatic
as
well
as
the l x planapochromatic objecti
ve
without
re
focusing. Otherwise turn the objective at the ri
ng
nut until
the object
is
focused.
~F
asten
the ring nutwith both screws.
Do
not overtight
en
both screws.
le
ica M
St
e
reom
i
croscop
es-
Asse
n
t>
ly
37

Illnrninators
Starting
up
38
Transformers
Follow
the
safety
instructions
given
on
page
7.
Leica
transformers
may
only
be
opened
by
authorized
service
personne
l,
and
the
power
cable
must
be
disconnected
before-
hand.
The
transformers
are
not
to
be
used
outdoors.
Regulating
transformer
The
regu
l
ating
transformer 0-7
V/40
VA
is
used
to operate the
6
V/10
Wand 6
V/20
W
lamps.
1
Voltage
selector
2 Connections for two
lamps
{maximum
comb
i
ned
loading
40
W)
3 Connection for power cable
4
Fuse
holder
F1.0
A
5 Power switch
I
on
0 off
6
ON
display: Instrument
is
switched
on.
7 Regulating
knob
8 6Vdisplay: The
maximum
voltage
has
been
reached.
~Set
the
available voltage
on
the voltage
se
l
ector.
-Position
115for
100
-
120
V
-Position
230
for 200-240 V
11>-
Connect
the
power
cable
to
the socket
of
the
base
and
to
the power source.
11>-
Conne
ct
the
l
amp
.
The
sockets accept two
lamps
with
a
maximum
comb
i
ned
loading
of
40
W.
Leica
M Stereomicroscopes
-I
ll
um
i
nat
i
ons
Switching
on
and
off
You
can
prolong
the
life
of
your
bulb
if
you
:
~
First switch off the
lamp
by
turning
the
rotary
knob
into
the black
range
~
and
only
then
switch the transformer
on
or
off.
~Turn
on
the
power switch.
The
diode
is
il
l
uminated.
~
Use
the rotary
knob
to
adjust t
he
brightness.
Above
6V
the
diode
is
illumi
nated.
Changing
fuses
Fuses
1.0A,
5x
20
{stock
no.
167
651)
~
Lamp
off.
~
Power switch off.
~Turn
the
fuse
holder counterclockwise
and
extract
it.
~
Fit
anew
fuse
and
replace the
fuse
holder.
In
the
event
of
~
Lamp
off.
overload
~
Power switch
off.
~Wait
afew seconds.
~
Power switch
on.
~Lamp
on
.
Step
transformer
Follow
the
safety
instructions
given
on
page
7.
•
You
can
attach a6
V/1
0W l
amp
to
the
step
transformer.
~Set
the voltage selector
on
the r
ea
r
side
to
115
V
or
230
V.
~Attach
the
power
cable
to
the power
supply.
~Con
n
e c
t
the
lamp.
~Adjust
thebright
ness
with the three-
st
ep
switch
{4/5/6
V).
Le1ca
M Stereomicroscopes - Ill
um
inations
39
This manual suits for next models
5
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