Optika Italy IM-5FLD User manual

Version: 1
Issued: 22, 03, 2018
Model
IM-5FLD
IM-5 Series
INSTRUCTION MANUAL

Page 2
Table of Contents
1. Warning
2. Symbols and conventions
3. Safety Information
4. Intended use
5. Overview
6. Unpacking
7. Assembling
8. Using the microscope
9. Transmitted Light Observation modes
10. Fluorescence Observation mode
11. Simultaneous Phase Contrast + Fluorescence Observation
12. Maintenance
13. Troubleshooting
Equipment disposal

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.

Page 4
5. Overview
FIELD STOP
LEVER
PHASE
CONTRAST SLIDER
TRINOCULAR
VIEWING TUBE
(FIXED)
DIOPTRIC
ADJUSTMENT
RING
EYEPIECE
LED
HOUSING
CONDENSER
COARSE
FOCUS KNOB
FINE FOCUS KNOB
TENSION
ADJUSTMENT
COLLAR
OBJECTIVE
STAGE
MICROSCOPE
BODY
LIGHT PATH
SELECTOR
LEVER
STAGE PLATE
LED CENTERING
KNOBS
X-Y STAGE
MOVEMENT
KNOBS
ECO BUTTON
TRANSMITTED
LIGHT INTENSITY
INDICATOR
CONDENSER
CENTERING
SCREWS
APERTURE
STOP LEVER
BRIGHTNESS
ADJUSTMENT
KNOB
(TRANSMITTED
LIGHT)
MAIN
SWITCH

Page 5
5. Overview (opposite side)
FLUORESCENCE LED
LAMP HOUSING
FLUORESCENCE
FILTER SELECTOR
REFLECTED LIGHT
DIAL
FLUORESCENCE
UV PROTECTION
SHIELD
FLUORESCENCE
FILTER INDICATOR
REFLECTED
LIGHT INTENSITY
INDICATOR
DIFFUSION
FILTER

Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
7. Assembling
Once you open the box, these are the microscope’s components:
① Microscope body
② Condenser
③ Stage extension
④ Mechanical stage
⑤ Phase contrast slider
⑥ Darkeld slider
⑦ Eyepieces
⑧ Centering telescope
⑨ Metal insert for stage
⑩ Glass insert for stage
⑪ Centering screws
⑫ Power supply
⑬ Power cord
⑭ Green lter (IF550)
⑮ Blue lter (LBD)
⑯ Dust cover
⑰ Holder for Petri diam. 38 mm
⑱ Holder for Terasaki and Petri diam. 65 mm
⑲ Holder for slide and Petri diam. 54 mm
⑳ Lens cleaning tissues
㉑Allen screwdriver
㉒UV protective shield
㉓ Fluorescence lter selector
㉔ Diffusion lter
㉕Lamp housing
⑪
⑩③
⑤
⑥
⑨
①
②
④
⑦
⑧
⑫
⑬
⑭
⑮
⑯
⑰
⑱⑲
⑳㉑㉒
㉓
㉔
㉕

Page 7
③
Fig.2
Fig.3
Fig.4
Fig.5
Fig.1
1. Turn the coarse focusing knob ① until the
nosepiece reaches its lowest position.
► For a safe transport, the nosepiece is
placed in the lowest position and the
tension adjustment collar ②is adjust-
ed to the appropriate tension when the
microscope leaves the factory. (Fig.1)
2. Screw the lowest magnication objective
on to the nosepiece from the right side,
then turn the turret clockwise. Install the
other objectives in the same way, following
the sequence from low to high magnica-
tion.
► Note: the objectives can also be in-
stalled through the stage opening.
(Fig.2)
► Clean the objectives regularly. In inverted
microscopes, the objectives are very sen-
sitive to dust.
► To prevent dust and contamination from
entering the microscope, cover all the un-
used holes with dust caps ③. (Fig.3)
► When operating, use the low magnication
objective (4x or 10X) to search and focus
the specimen, then switch to higher magni-
cations.
► When switching between objectives, slowly
turn the nosepiece until it clicks. The click
means that the objective is in the right posi-
tion, in the center of the light path.
①
②
7.1 Installing the objectives
The stage extension can be installed on either
side of the stage to enlarge the working sur-
face. The mechanical stage must be installed
on the right side of the microscope.
1. Installing the stage extension: screw the
bolts on to the extension, then mount the
extension from below the stage. (Fig.4)
2. Installing the mechanical stage: as for the
extension, the mechanical stage is xed
with two bolts under the stage. (Fig.5)
7.2 Installing the stage extension
and the mechanical stage

Page 8
1. When using the stage inserts, make sure
that the insert is horizontal.
2. Install the stage insert in the stage open-
ing. (Fig.6)
7.3 Installing the stage insert
7.4 Installing the eyepieces
Insert both eyepieces into the tubes of the
optical head. (Fig.7)
Fig.6
Fig.7
Fig.8
1. Slide the condenser into the condenser
holder, inserting the pin on the rear side of
the condenser with the alignment groove
in the condenser holder. (Fig. 8)
2. Lock the condenser with the locking knob
① on the right side of the condenser hol-
der. (Fig. 9)
7.5 Installing the condenser
Fig.9
①
1. Turn the main switch ① to “O”(o󰀨) before
connecting the power cord. (Fig.10)
2. Insert the plug of the provided power
supply into the power socket of the
microscope. (Fig.11)
3. Plug the power cord int the socket of the
power supply (Fig.12)
4. Plug the power cord into the mains socket.
Check for a safe connection.
► Please use the supplied power cord.
If lost or damaged, please refer to
qualied service.
► Connect the power cord to a grounded
power supply only.
7.6 Connecting the power cord
Fig.10
Fig.11 Fig.12
①

Page 9
1. Unscrew the lamp housing lock (Fig. 13)
2. Insert the lamp housing in the
uorescence illuminator connector in the
back side of the microscope. (Fig.14)
3. Tighten the locking screw using the
provided allen screwdriver. (Fig.15)
4. Connect the cable of the lamp hoising
to the connector in the back side of the
microscope. (Fig.16)
7.7 Installing the lamp housing
Fig.13
Fig.14
Fig.15
Fig.16

Page 10
Turning on the light source
Connect the power, turn on the main switch ①.
(Fig.17)
Adjusting the brightness
Turn the brightness adjustment knob ②to
increase and decrease the brightness. (Fig.18)
While rotating the brightness adjustment knob,
the LED indicator on the front panel ③ will turn
ON or OFF some segments. (Fig.19)
Adjusting the tension
► The coarse focusing knob ①is pre
adjusted to a tight tension upon
leaving the factory.
If the nosepiece drops down by itself, or the
specimen defocuses while adjusting the ne
focus knob ③, the coarse focus knob ①is too
loose. Turning the tension adjustment collar
② in clockwise direction tightens the coarse
focus tension. Rotate in the opposite direction
to decrease the tension. (Fig.20)
Setting the specimen
► For the best image quality, use asks,
Petri dishes and slides with a 1.2 mm
thickness.
1. Place the proper insert for your specimen
(according to the table on the right) on the
stage, and x it with the stage clip.
2. Turning the X and Y knobs, move the
specimen to the required position. (Move-
ment Range: 120 (width) × 78 (length)
mm).
Moving the specimen
Move the specimen to the desired position by
freehand or by turning the knobs of the me-
chanical stage.
► When switching objectives, take care
not to touch the adaptor plates with
the objectives, as their weight may
damage the front lens.
8.1 Initial setup
8.2 Stage
8. Using the microscope
Fig.17
Fig.18
①
②
①
②
③
Fig.20
Fig.19
Holder for Petri diameter 38 mm (holder for Terasaki
needed) (provided with the microscope)
Holder for Terasaki and Petri diameter 65 mm
(provided with the microscope)
Holder for slide and Petri diameter 54 mm
(provided with the microscope)
M-793.4
Holder for 2+2 slides.
M-793.6
Holder for Utermöhl-Chamber (holder for Petri 54mm
needed)
Load-bearing side extension
(provided with the microscope)
③