Q-sense E4 User manual

Dec 2007, Rev B
©Q-SENSE AB 2007
Q-SENSE E4 LIQUID HANDLING SET UP
Q-Sense E4 Flow System Setup, with IPC-N Pump
Part 1 – Ismatec IPC-N Pump
Before switching the pump on,
check the voltage setting and fuse
on the backside:
220-240V: use 250mA fuses
110-120V: use 500mA fuses
Keep the “SETTINGS” button
pressed while switching the pump
on. This makes the pump start in
settings mode (the blue words
above the buttons are active).
Cycle through the settings, using
the up/down arrows, until “TUBE” is
shown, select by pressing “OK”.
Select inner diameter 0.64 mm,
(double-check with numbers given
on the tubing itself!). Press “OK” to
confirm.
Press “ESCAPE” to save settings.
Attach the adaptors on each
cassette, and then the tubing.
Ready to go!
Typical flow rates: for filling the
system 100-400 μl/min; for
experiment 25-100 μl/min.
50 μl/min
100 μl/min
For details and calibration function, see supplier’s manual.
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Voltage settings
window

Dec 2007, Rev B
©Q-SENSE AB 2007
Q-SENSE E4 LIQUID HANDLING SET UP
Part 2 –Tubing connectors
The ferrule forms a tight seal as it
is deformed when being screwed
into the flow module. Place the
ferrule and threaded connector
as shown before attaching them.
Note that the arrows on the top of
the module indicate the direction
of the flow for correct sample
temperature equilibration.
Use the brown adapter (the
perifit) to connect to the soft
pump tubing (expanding the
narrow bore of the pump tubing
with a pipette tip may help). If
1.5-2mL tubes are used, the
white lids can be used to guide
the tubing. Note that the lid needs
to be vented to work properly.
Place the pump and sample
holder so that the samples are
drawn through the E4 system.
This setup will reduce the need
for pump tubing cleaning and
prevent from leakage accidents.
For details, please check the E4 Operator Manual.
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In
Out
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