RSLAB HiFuge-GJ6 User manual

Version 1, March 2019 User Manual centrifuge GJC006
User Manual
RSLab microtubes centrifuge, model HiFuge-GJ6
Code: GJC006

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Version 1, March 2019 User Manual centrifuge GJC006
Contents
1. Specifications ...............................................................................................................................................2
2. Required Operational Condition....................................................................................................................2
3. Installation....................................................................................................................................................2
3.1 Location.............................................................................................................................................3
3.2 Connection of the power cord and grounding......................................................................................3
4. Structure....................................................................................................................................................... 4
5. Operation panel.............................................................................................................................................5
6. Rotor Preparation..........................................................................................................................................6
6.1 Prepare the samples.............................................................................................................................6
6.2 Inject the samples into tubes...............................................................................................................6
6.3 Keep the tubes balance........................................................................................................................6
6.4 Inspect the rotor .................................................................................................................................. 6
6.5 Symmetrically load centrifuge tubes in rotor........................................................................................ 6
7. Operation......................................................................................................................................................7
7.1 Normal Operation ............................................................................................................................... 7
7.2 RCF Operation.................................................................................................................................. 10
7.3 Pulse Operation................................................................................................................................. 10
8. Maintenance ............................................................................................................................................... 10
9. Troubleshooting.......................................................................................................................................... 12
9.1 Frequent problems list....................................................................................................................... 12
9.2 How to open the door........................................................................................................................ 13
10. Instructions of rotor and tube..................................................................................................................... 14
10.1 The rotor instructions................................................................................................................... 14
10.2 Tubes........................................................................................................................................... 16
11. Calculation Relative Centrifuge Force（RCF）........................................................................................ 18

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Version 1, March 2019 User Manual centrifuge GJC006
1. Specifications
Maximum speed
15000rpm, increment: 100rpm
Maximum RCF
15100×g, increment: 100×g
Maximum capacity
2ml×12
Timer
30 seconds -99 minutes-HOLD, continuous operation
Driving Motor
Brushless DC motor
Safety devices
Door interlock、over-speed detector、over-temperature detector、automatic
internal diagnosis
Power requirements
Single-phase, 110V-240V, 50Hz/60Hz, 3A
Dimensions（mm）
（L）255×（W）245×（H）140
Weight
6kgs
Additional features
Speed/RCF switch、Pulse operation、Processing display、Voice reminder
2. Required Operational Condition
3.1 Basic operational Conditions
（1）Power: 110V-240V, 50Hz/60Hz, 3A.
（2）Ambient temperature: 2℃-40℃.
（3）Relative humidity: ≤80%.
（4）No vibration and airflow around.
（5）No electric dust, explosive and corrosive gases around.
3.2 Transport and storage condition
（1）Storage temperature: -40℃-55℃.
（2）Relative humidity: ≤93%.
3. Installation
This section describes the instructions that you should abide when install the centrifuge to ensure your
safety and the optimum performance. Before moving the centrifuge, the rotor must be removed.

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Version 1, March 2019 User Manual centrifuge GJC006
WARNING：
⚫Improper power supply may damage centrifuge.
⚫Make sure the power source conforms to the required power supply before connecting.
3.1 Location
（1）Place the centrifuge on a firm, flat and level table, ensure the four feet of this centrifuge stand on the
table firmly. Avoid installing on the slippery surface or surface prone to vibration.
（2）Ideal ambient temperature is 20℃±5℃, avoid placing the centrifuge in direct sunlight if temperature
exceeds 30℃.
（3）Keep clear of the centrifuge at least 10cm on both sides and at least 30cm behind it to guarantee the
cooling efficiency.
（4）Keep away from heat or water to avoid sample temperature issues or centrifuge failures.
3.2 Connection of the power cord and grounding
WARNING：
⚫Toavoid electrical shocks, ensure your hands are dry when touching the power cord.
⚫This centrifuge must be grounded properly.
A minimum 10A outlet providing a sufficient ground is required, and this must meet with local safety
requirements.

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Version 1, March 2019 User Manual centrifuge GJC006
foot
Door release hole
LCD Display
Rotor
Door lock hook
4. Structure
Figure 5.1 Front view of centrifuge
Figure 5.2 Rear view of centrifuge
Operation panel
View
Power switch
Power inlet
Air vents
Door

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Version 1, March 2019 User Manual centrifuge GJC006
12 min
×100rpm
150
5. Operation panel
Figure 6-1 Operation Panel
Item
Symbol
Name
Function
1
Select button
Press the button to choose the program which you want to
modify.
2
Pulse button
The speed can be accelerated and held at the speed when
pressing Pulse on.
3
Parameter button
Clockwise rotate to increase program values. Rotate anti-
clockwise to decrease parameter values.
Press the button, shift between speed and RCF display.
4
Open/ lock button
Press the button to open the door The button is not available
when the centrifuge is running.
5
Start/ Stop button
Press the button to start running. The centrifuge will brake to
stop running if pressed during centrifugation.
Speed area Lock status Time area
Figure 6-2 the main interface
Main interface is as figure 6-2. The speed is set to be 15000 rpm, running time is 12 minutes. When speed
symbol is rotating, indicating the centrifuge is running. Time display symbol display the ratio of
working to time setting. The total time setting is divided into 10 sections.

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Version 1, March 2019 User Manual centrifuge GJC006
6. Rotor Preparation
6.1 Prepare the samples
6.2 Inject the samples into tubes.
CAUTION：
⚫Do not overload samples into the centrifuge which will cause leaking.
⚫Do not exceed the actual capacity allowed in the user manual.
6.3 Keep the tubes balance
⚫Although the centrifuge can accept sample balancing by eye, we recommend that you keep this
centrifuge in a well-balanced condition to extend its life expectancy.
⚫Never intentionally run the centrifuge under unbalanced condition even though the allowable
imbalance is not exceeded.
6.4 Inspect the rotor
Check the rotor for corrosion or scratches before using.
CAUTION：
⚫Any abnormality such as corrosions or scratches are found, stop using the rotor and contact our service
center.
⚫Only manufacturer’s rotors must be used with the unit.
6.5 Symmetrically load centrifuge tubes in rotor
CAUTION：
⚫Make sure the rotor lid is securely fixed on the rotor, as well as the rotor and shaft are tightened.
Otherwise, the rotor may be moved off while rotating and cause damage of the centrifuge and rotor.

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Version 1, March 2019 User Manual centrifuge GJC006
7. Operation
CAUTION：
⚫Do not push or lean against the centrifuge while it is running.
⚫Do not run the centrifuge when fragments or sample solutions are left in the centrifuge chamber.
Always keep the centrifugal chamber clean.
⚫If the centrifuge makes strange noise during operation, stop it immediately and contact our service
center. Notify them of the warning code if displayed.
7.1Normal Operation
Turn on the power switch, centrifuge will display the running interface last time after passing the self-
diagnostic checks, see figure 8-1 below:
Figure 8-1 the last running interface
⚫Speed: 15000rpm. Running time: 12 minutes.
⚫The door lock is released.
7.1.1 Rotor loading and removal
CAUTION
⚫Attach the rotor to the rotor shaft. Ensure the rotor is in position and connected with the shaft,
tightening the locking nut to secure the rotor with shaft, to prevent the rotor damaging the centrifuge.
⚫Ensure the rotor lid is firmly tightened to the rotor.
12 min
150×
100rpm

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ach the lockin
Figure 8-2 the rotor installation
7.1.1.1 Load the rotor to the shaft to ensure rotor is in position until it connected well with the shaft.
7.1.1.2 Rotate the rotor slightly by your fingers to check, if the rotor vibrates, if so attach the rotor
again.
7.1.1.3 Hold on the rotor with one hand, tighten clockwise the nut with the other hand, and make
sure tighten firmly.
7.1.1.4 Put the rotor lid to the rotor, press the fastener down and make the
fastener att tightly.
7.1.1.5 Close the door and start running.
Fastener g nut
7.1.1.6 To release the rotor, firstly pull the fastener up to remove the lid, and then turn the locking
nut anti-clockwise.
CAUTION
⚫Check the rotor is firmly tightened before running.
7.1.2 Set the operation parameters
Press the button to select required parameters. The parameter can be modified when the parameter is
flashing. Clockwise rotate the parameter button to increase parameter value; counter-clockwise rotate the
parameter button to decrease parameter value. Parameter button rotate faster, parameter value
increase faster. The minimum speed increment is 100 rpm, the minimum time increment is 1 second.
（1）Set the speed
⚫Press the select button until the speed rpm is displayed.
⚫When the speed button is selected, the speed symbol will flash the speed value.
⚫The minimum speed value you can set 500rpm, the minimum increment is 100rpm.
⚫Rotate program button clockwise to increase speed value, rotate the program button anti-clockwise
to decrease speed value.
⚫You can speed-up set the speed value by rotating program button quickly.
⚫There is a circulating function to increase/decrease the speed values. Rotate the program button

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Version 1, March 2019 User Manual centrifuge GJC006
clockwise to change settings from small → large → maximum → minimum. Rotate the program
button anti-clockwise to change settings from large → small →minimum → maximum.
（2）Set the time
⚫Press button , time value flashes in the time setting mode.
⚫Rotate the program button to set running time from 30 seconds to 99 minutes.
⚫When time displays HD, this is a continuous running mode.
7.1.3 Start the operation
（1）Press running button to start running
⚫Timer will operate once the speed setting value is reached, the screen displays the remaining run time.
（2）View and modify the operation programs
⚫Pressing button , returns the display to the program interface and displays settings programs. Press
the select button to the desired program. When flashing, rotate parameter button to modify
values. Release the button after 5 seconds, and the centrifuge will return to normal operation mode and
run according to the new value.
⚫If the set time value has been modified, the operation time is not affected and will continue.
（3）Warning display
⚫If an error occurs during the operation, the centrifuge will brake to stop automatically, and display the
error code on the time/display area. The error code can be checked in the table 10-1, and corrective
actions can be applied accordingly.
7.1.4 End the operation
（1）The centrifuge will brake when it reaches the setting time or button is pressed.
⚫When the rotor stops rotating, centrifuge will start beeping to alert the operation has finished.
（2）Open the door
⚫The door can be released automatically when the operation has stopped.
⚫With the door closed, you are able to press the button to open it.
⚫After ending the operation, the program will store the setting parameters of this operation, and will
recall these parameters when restart the program.
（3）Open the door and take out the rotor and samples.

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7.2 RCF Operation
（1）Turn on the power switch.
（2）Set a RCF (Relative Centrifugal Force) value.
⚫Press the select button and choose speed unit ×g, the speed symbol will flash into RCF value
input status.
⚫If no button is pressed after the speed value has flashed for 5 seconds, the input mode will be shut
down.
⚫Rotate program button to input a RCF value, RCF increment is100×g.
（3）Set operating conditions
⚫The other operation, please refer to the section 8.1.
7.3 Pulse Operation
This function is used to remove the residual samples adhered on the interior of the tubes.
（1）Turn on the power switch and load the rotor to the shaft, tighten the rotor lid and make sure it is in
secured position, and then close the door.
（2）The centrifuge gets into preparation mode and displays last running values. The values can be reset.
（3）Press knob and hold, the centrifuge will speed up to the setting speed. While releasing
knob during acceleration, the centrifuge will start to decelerate and stop.
8. Maintenance
CAUTION
⚫If do not follow the recommended instructions for cleaning or disinfecting may damage the centrifuge.
（1）Centrifuge
⚫If the centrifuge is exposed to ultraviolet rays for a long time, the color of the doors may be changed or
the label may be came off. After using, cover the centrifuge with a piece of cloth to protect it from
direct exposure.
⚫If the centrifuge needs cleaning, clean it with a cloth or sponge moistened with a neutral detergent
Note：The button works only when the rotor stopped and the door is locked.

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Version 1, March 2019 User Manual centrifuge GJC006
solution.
⚫Sterilize the centrifuge by wiping with a cloth moistened with 70% ethanol solution.
（2）Rotor chamber
CAUTION
⚫Do not directly pour water, neutral detergent or disinfectant solution into the rotor chamber. Otherwise
fluids may leak into the drive units and cause corrosion or deterioration to the bearings.
⚫If the rotor chamber needs cleaning, clean with cloth or sponge moistened with a neutral detergent
solution. Sterilize the centrifuge by wiping with a cloth moistened with 70% ethanol solution.
（3）Drive shaft
⚫We recommend regular maintenance for drive shaft. Youcan wipe the drive shaft with soft cloth, and
then apply a thin coat of silicon grease.
（4）Door
⚫Clean and sterilize the door using the same method as the step (1) above.
（5）Rotor
⚫Toprevent corrosion, remove the rotor from rotor chamber. If not in use for a lone term, then detach
the rotor lid and turn upside down to dry the tube holes and keep clean.
⚫For sample leaks in the rotor, rinse the rotor with water. Apply a thin coat of silicon grease to the rotor
when it is completely dry.
⚫The rotor should be regular maintenance, recommend to cleaning it each 3 months to ensure tube and
rotor holes keep clean, and then apply a thin coat of silicon grease.

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9. Troubleshooting
9.1 Frequent problems list
This centrifuge has a self-diagnostic function. If a problem occurs, an error/warning code will be displayed
on the time display screen and the operator can determine the malfunction with the warning code below.
Symptom
Causes
Solutions
Nothing appears on the screen when
the POWER is turned on.
·Building power circuit
breaker trips.
·the fuse was blown out.
·Remove the trouble and turn
on the POWER.
·Replace the fuse.
Abnormal vibration
·Rotor do not match with
spindle
·Samples are imbalance
·Install again the rotor
·Weighting scales, install
symmetrically

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·Rotor lid loosed
·Tighten the rotor lid firmly
Alarm code appeared on the time
display screen
E-02
Door fault
·The door opened in running.
·The button is pressed
while the door opening.
·Close the door immediately.
·Close the door，and then
start to operate.
E-06
Set wrong speed
·The setting speed exceed the
allowable range.
·Modify the speed value.
E-10～86
·Read the service manual
·Contact with service center
Table 10-1 Frequent problems and solutions
⚫Warning code E-1～9 are related to wrong operating. Youcan continue running the centrifuge after the
cause removed.
9.2 How to open the door
9.2.1 In the case of poweron
9.2.1.1 Turn on the POWER switch, the door lock will release automatically.
9.2.1.2 The door lock will release automatically once the operation finished.
9.2.1.3 It is available to release the door by pressing button once the rotorstops.
9.2.2 In the case of power outage
The door cannot be opened automatically if there is a power outage. It is available to be opened manually.
（1）Ensure if the rotor has stopped rotating.
⚫Listen carefully to ensure no rotating sound can be heard.
（2）Insert a screw driver into the hole to open the door.
⚫Hole is located on the top right side of the unit.
⚫Insert a screw driver into the hole and push forward to release the door.
CAUTION
⚫The door just can be opened while the power on and rotor stops rotating.

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10. Instructions of rotor and tube
CAUTION：
⚫Read the instructions thoroughly, correct use rotor.
⚫Do not exceed the allowable maximum speed of rotor、tube and adapters etc., be care that the allowable
maximum speed of some adapters are lower than the rotor’s maximum speed.
10.1The rotor instructions
10.1.1 Rotor structure
Locking nut Fastener Lid
Rotor block Spindle hole Tube
Figure11-1 the rotor profile

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10.1.2Available rotors and adapters
Table 11.1 Rotors and adapters
Model
Rotor
type
ID
code
Tubes
Adapters
Maximum
speed（rpm）
Maximum
RCF（×g）
Allowable imbalance（*）
imbalance
Volume
imbalance（**）
D2012
A12-2
01
1.5/2.0ml
tube
15000
15100
2.0g/tube
5mm/ tube
0.2ml PCR
tube
A02P2
15000
11700

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0.5ml micro
tube
A05P2
15000
12780
D2012
plus
A12-2P
02
1.5/2.0ml
tube
15000
15100
2.0g/ tube
5mm/ tube
0.2ml PCR
tube
A05P2
15000
11700
0.5ml micro
tube
A02P2
15000
12780
10.1.3 Notice
⚫The centrifuge rotor can separate sample which density lower than 2.0g/ml. if the separated samples
density is over 2.0g/ml, please calculate allowable speed depending on the following formula.
Allow Speed (rpm)= Maximum speed×(2.0(g/ml)/Sample density (g/ml)) 1/2
10.1.4Autoclaving
A12-2 rotor is manufactured in high-strength aluminum alloy material and can be autoclaved: 121℃(1.0kg/cm2),
20 minutes.
A12-2P rotor is made of plastic, cannot be high-pressure sterilization and UV irradiation, only ordinary
sterilization can be used.
CAUTION:
⚫The lid of the rotor is made of plastics, can not be high-pressure sterilization, only ordinary sterilization
can be used.
10.2 Tubes
10.2.1 Cleaning and sterilizing tubes
Table 11.2 Cleaning and sterilizing conditions for tubes
O：Applicable X：Inapplicable
Condition Material
PA
PC
PP
Cleaning
Cleaning fluids
Acidic（pH5 or lower）
X
X
X
Acidic（higher than pH5 ）
O
O
O
Alkaline（higher than pH9 ）
O
X
O
Alkaline（pH9 or lower）
O
O
O
Neutral（pH7）
O
O
O
Warm water(up to 70℃)
O
O
O
Ultrasonic cleaning
Neutral detergent（pH7）
O
O
O

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Sterilization
Autoclaving
115℃（0.7kg/cm2）30minutes
O
O
O
121℃（1.0kg/cm2）20 minutes
X
O
O
126℃（1.4kg/cm2）15 minutes
X
X
X
Boiling
15 to 30 minutes
O
O
O
Ultraviolet sterilization
200-300nm
X
X
X
Gas sterilization
Ethylene oxide
O
X
O
Formaldehyde
O
O
O
PA: Polyallomer；PC：Polycarbonate；PP：Polypropylene
10.2.2 Cleaning PC tubes
PC materials are low in chemical resistance against alkaline solutions. Avoid using neutral detergents with
pH higher than 9. Note that pH of some neutral detergents are still higher than 9 even if diluted according to
the instruction in the maker’s catalog. Use detergent with its pH between 7 and 9.
10.2.3Autoclaving PA、PC and PP tubes
PA begins softening at about 120℃, PC and PP at about 130℃. Autoclave PA tubes at 115℃（0.7kg/cm
2
）for
30 minutes and PC and PP tubes at 121℃（0.1kg/cm2）for 20 minutes. If a certain temperature is exceeded,
the tubes may be deformed.
When using a sterilizing chamber, please operate as follows:
10.2.3.1 Place tubes in vertical position, mouths upward. If tubes are placed sideways, they may
deform into an oval shape due to gravity.
10.2.3.2 Remove screw nuts and inner covers to prevent from deformation or rupture.
10.2.3.3 Wait until the sterilizing chamber cools down to the room temperature before the tubes
are removed.
10.2.4 Condition and life expectancy of tubes
The life expectancy of plastic tubes depends on the characteristics of samples, speed of the rotor used, and
temperature applied, and so on. When the plastic tubes are used for centrifuge of ordinary aqueous samples
(pH between 5 and 9), their life expectancies are defined as follows.
Be operated at the maximum speed:
High quality tubes (PA、PC、PP): 30-50 operations
Ordinary tubes(PA、PC、PP): around 10 operations（Using in low speed can extend the tube life）.
Life expectancy of tubes also depends on the pretreatment conditions such as cleaning and sterilization,
lifetime can be cut down.
Notice: Do not use damaged or cracked tubes.

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11. Calculation Relative Centrifuge Force（RCF）
Relative Centrifuge Force (RCF) can be determined with the following calculation formula.
RCF=1.118×r×n2×10-5
R—rotating radius, unit: cm; n—rotating speed, unit: rpm