Sakura Tissue-Tek Xpress x120 User manual

Operating
Operating
Manual
Manual
Rapid Tissue Processor
Tissue-Tek® Xpress
®
x120

Operating
Operating
Manual
Manual
Rapid Tissue Processor
Tissue-Tek
®
Xpress
®
x120

© 2012 Sakura Finetek USA, Inc.
All Rights Reserved
Printed in U.S.A.
0004389-03 Rev.C
Manufactured for:
Sakura Finetek U.S.A., Inc.
Torrance, CA 90501 U.S.A.

TABLE OF CONTENTS
Revised 10/02/2012 iii
Section Page
1 INTRODUCTION
Intended Use ................................................................................................................................................ 1.1
General Description...................................................................................................................................... 1.1
Safety Precautions ....................................................................................................................................... 1.2
Location of Major Systems and Components ............................................................................................... 1.6
Specifications ............................................................................................................................................. 1.12
Safety Standards ........................................................................................................................................ 1.13
2 INSTALLATION
Turning Instrument Power On or OFF .......................................................................................................... 2.1
General Information ...................................................................................................................................... 2.1
Environmental Factors.................................................................................................................................. 2.1
Set Reagent Bottle ....................................................................................................................................... 2.2
Pre-Start Checks .......................................................................................................................................... 2.2
Unpacking and Installation............................................................................................................................ 2.3
3 CUSTOMIZATION OF SETTINGS
General Information...................................................................................................................................... 3.1
Accessing the UTILITIES Menu.................................................................................................................... 3.1
Configuring System Settings ........................................................................................................................ 3.2
4 OPERATING INSTRUCTIONS
Initial Setup................................................................................................................................................... 4.1
Understanding the Control Panel Display..................................................................................................... 4.1
Preparation for Processing ........................................................................................................................... 4.2
System Start Up ........................................................................................................................................... 4.3
System Operation......................................................................................................................................... 4.5
Special Conditions and Considerations ...................................................................................................... 4.12
Viewing Reports and Statistics ................................................................................................................... 4.13
5 RECOVERY PROCESS
Overview....................................................................................................................................................... 5.1
Detecting an Error Condition ........................................................................................................................ 5.1
Starting a Processing Run in Retort 3 or 4 ................................................................................................... 5.2
6 CONSUMABLES AND ACCESSORIES
Consumable Materials.................................................................................................................................. 6.1
Standard Accessory Items............................................................................................................................ 6.1
Maintenance and Service Items ................................................................................................................... 6.2
Accessory Items for Compatibility with the Tissue-Tek ® AutoTEC ® Embedding System ........................................ 6.2

TABLE OF CONTENTS
iv Revised 10/02/2012
Section Page
7 CARE OF THE INSTRUMENT
General Information...................................................................................................................................... 7.1
On Condition Maintenance ........................................................................................................................... 7.1
Daily Maintenance ........................................................................................................................................ 7.7
Weekly Maintenance .................................................................................................................................... 7.8
Monthly Maintenance.................................................................................................................................. 7.11
Routine Maintenance Check List ................................................................................................................ 7.13
8 TROUBLESHOOTING
General Troubleshooting Procedures........................................................................................................... 8.1
Monitored Errors........................................................................................................................................... 8.2
9 SERVICE AND REPLACEMENT PARTS
Service Information....................................................................................................................................... 9.1
Consumable Materials.................................................................................................................................. 9.1
Replacement Accessory Items ..................................................................................................................... 9.1
Service Accessory Items .............................................................................................................................. 9.1
Optional Accessory Items............................................................................................................................. 9.2
APPENDIX Tissue-Tek®Xpress®x120 Rapid Tissue Processor Applications Manual
Overview.......................................................................................................................................................A.1
Preparation for Processing ...........................................................................................................................A.1
Use of Tissue-Tek Xpress x120 Pre-Processing Solution or Pre-Processing Fixative .................................A.1
Use of Tissue-Tek Molecular Fixative...........................................................................................................A.2
Embedding of Tissue-Tek Xpress x120 Processed Tissue ..........................................................................A.4
Microtomy .....................................................................................................................................................A.4
Staining.........................................................................................................................................................A.4
Microscopic Evaluation.................................................................................................................................A.5
Reprocessing of Tissue ................................................................................................................................A.5
Tissue-Tek Xpress x120 Reagent Replacement Schedule .........................................................................A.5
Do’s and Don’ts ............................................................................................................................................A.6
Disposal of Tissue-Tek Xpress x120 Processing Solutions..........................................................................A.6
Tissue-Tek Xpress x120 Processing Flow Chart for Standard Program.......................................................A.7
Tissue-Tek Xpress x120 Processing Flow Chart for Extended Program......................................................A.7

Revised 10/02/2012 1.1
Section 1
INTRODUCTION
Intended Use
The Tissue-Tek®Xpress®x120 is designed for the
purpose of processing human and animal tissue speci-
mens.
The instrument, as part of the histopathology process,
is intended to facilitate the in vitro examination of human
and animal tissue for morphology changes by a
pathologist.
General Description
The Tissue-Tek® Xpress®x120 Continuous Rapid Tis-
sue Processor is a self-contained tissue processor
employing microwave and vacuum infiltration techniques.
The instrument performs automatic rapid processing
(fixation, dehydration, clearing, and paraffin impregnation)
of tissue specimens in preparation for histological study
and examination. The instrument incorporates individual
processing stations, scheduling software, and an auto-
mated transfer system that provides for continuous
processing of multiple sample baskets. Processing time
for a single basket is approximately 65 minutes, with a
maximum throughput (during continuous processing) of
120 specimens per hour.
NOTE: Maximum throughput is based on processing 40
cassettes per basket, where processing time for all
processes is equal (15 minutes). Reduction in the number
of cassettes processed per basket, or an increase in
processing time will lower throughput.
In order to achieve continuous processing, the instru-
ment utilizes a Transfer System that sequentially moves
baskets containing tissue specimens from a Loading
Station, through the four processing retorts to an Unload-
ing Station for removal of the basket. The instrument
employs scheduling software to efficiently process and
transfer sample baskets.
The Tissue-Tek Xpress x120 instrument has the follow-
ing features:
•Instrument can be configured for either Manual or
Automatic start-up
•Compatible specimen types include Formalin fixed
tissue, tissue fixed in Tissue-Tek Xpress Molecular
Fixative
•Supports use of standard cassettes and Tissue-Tek®
Paraform®cassettes (both microwavable); compatible
cassettes include:
Standard cassette (Tissue-Tek® Brand Uni-Cassette®
Cassettes)
Biopsy cassette (Tissue-Tek® Brand Uni-Cassette®
Biopsy Cassettes)
Tissue-Tek®Brand Paraform®Standard cassette
Tissue-Tek®Brand Paraform®Biopsy cassette
Tissue-Tek®Brand Paraform®Biopsy 13 x13 cassette
Tissue-Tek®Brand Paraform®Biopsy Core cassette
Tissue-Tek®Brand Paraform®Biopsy Shaved cas-
sette
Tissue-Tek®Brand Paraform®Orientation cassette
•Standard cylindrical basket with lid facilitates
processing of up to 40 cassettes per basket; upper
loading limit of basket is marked to aid in positioning
cassettes
•Tissue-Tek®Paraform®magazines are compatible for
use with the instrument (for use in conjunction with
the Tissue-Tek®AutoTEC®Automated Embedding
System). Each Tissue-Tek Paraform magazine holds
up to 20 Paraform®cassettes. Two magazines linked
by a handle can be placed in the Loading Station of
the Tissue-Tek Xpress x120.
•Two user-selectable automated processing programs
based on specimen thickness, Standard Program (15
minutes per retort) or Extended Program (30 minutes
per retort).
•Maximum throughput of 120 cassettes per hour
(under Standard Program, where each basket or pair
of magazines processed contains 40 cassettes)
•Integral fume control system to prevent fumes from
escaping the instrument

INTRODUCTION
1.2 Revised 10/02/2012
Safety Precautions
NOTES, CAUTIONS, WARNINGS, and other safety
related labeling are provided throughout this manual to
indicate levels of potential hazards as defined below:
NOTE Indicates a reminder or other helpful
information.
CAUTION Indicates a potential hazard in which
failure to follow instruction may result in
damage to the Tissue-Tek® Xpress®
x120 and/or other property, or may give
poor processing results.
WARNING Identifies a potential hazard in which
failure to follow instructions may result in
serious injury to the operator and/or
other personnel.
HOT SURFACE Indicates hot surfaces. Take precautions
to prevent burns.
BIOHAZARD Possibility of infections depending on
the type of specimens processed. Pre-
vent infections by using Personal Pro-
tective Equipment (PPE) as required by
OSHA and any applicable state or local
regulations.
CAUTION: Do not use cassettes that contain metal or
use metal lids. Do not place any metal items in Retorts
#1 and #2.
CAUTION: When placing cassettes into the basket, do
not allow cassettes to extend above the upper limit
band.
CAUTION: The instrument uses flammable liquids. Do
not use an open flame near the instrument.
CAUTION: When operating the instrument or when
handling contaminated, bio-hazardous materials use
Personal Protective Equipment (PPE) as required by
OSHA and any applicable state or local regulations.
CAUTION: Operating the instrument for a use other
than described in the operating manual could result in
hazardous conditions.
CAUTION: Do not remove the Outer Panels on the
instrument.

Revised 10/02/2012 1.3
Cabinet Front (Figure 1-A)
Loading Station Access Door ..1.. — provides access to
the Loading Station to allow loading of tissue specimens
for processing. Access to the Loading Station is permitted/
denied, under software control, depending on the current
state of the instrument (see “Loading Indicator”, below, for
details). A switch associated with the access door serves
as an interlock to immediately remove power from the
Transfer System motors (see “Transfer System (Figure 1-M)”
on page 1.11 for details) in the event the door is opened
while a transfer is in process. An alarm sounds any time
the Loading Station access door is open, even though the
Loading Indicator is off. An alarm also sounds if the
Loading Station access door is left open for 30 seconds or
more while the Loading Indicator is illuminated.
Loading Indicator ..2.. — provides an indication of the
status of the Loading Station access door:
Light Off – indicates “access denied” to the Loading
Station access door; the door should not be opened.
The Loading Indicator is “off” whenever there is a
basket in the Loading Station or when the Transferring
Arm is in motion.
Light On – indicates “access permitted” to the Loading
Station access door; the door can be opened. The
Loading Indicator is “on” when there is no basket in the
Loading Station and the Transferring Arm is stationary.
The Loading Indicator is operational only when a user is
logged onto the system.
Unloading Station Access Door ..3.. — provides access
to the Unloading Station to allow unloading of processed
tissue specimens. Access to the Unloading Station is
permitted/denied, under software control, depending on
the current state of the instrument (see “Unloading
Indicator” on page 1.4 for details). A switch associated
with the access door serves as an interlock to immediately
remove power from the Transfer System motors (see
Figure 1-M on page 1.11 for details) in the event the door
is opened while a transfer is in process. An alarm sounds
any time the Unloading Station access door is open, even
though the Unloading Indicator is off. An alarm also
sounds if the Unloading Station access door is left open
for 30 seconds or more while the Unloading Indicator is
illuminated.
Figure 1-A

INTRODUCTION
1.4 Revised 10/02/2012
Unloading Indicator ..4.. — provides an indication of the
status of the Unloading Station access door:
Light Off – indicates “access denied” to the Unloading
Station access door; the door should not be opened. The
Unloading Indicator is “off” whenever the Unloading
Station is empty or when the Transferring Arm is in
motion.
Light On – indicates “access permitted” to the Unloading
Station access door; the door can be opened. The
Unloading Indicator is “on” when there is a basket in the
Unloading Station and the Transferring Arm is stationary.
Flashing – alerts the operator to remove the basket(s)
from the unloading station or the access door is open
and needs to be closed immediately.
Audible Alarm (not shown) – indicates the following:
•A tone sounds at the completion of a processing cycle
(when a basket has arrived at the Unloading Station).
Tone selection (from seven available tones), volume
selection (high, middle, low), and pattern selection
(continuous, intermittent, 30 seconds only) are user
selectable (see “Configuring System Settings” on page
3.2 for details).
•A continuous tone sounds if an abnormal condition is
detected during processing. The alarm condition must
be acknowledged and/or cleared by the operator to
cancel the alarm. Tone selection (from seven availa-
ble tones) and volume selection (high, mid, low) are
user selectable (see “Configuring System Settings” on
page 3.2 for details).
Retort Access Door ..5.. — provides access to the
Microwave and Vacuum Stations for purposes of cleaning
and/or maintenance. Access to all four retorts is permit-
ted/denied, under software control, when the instrument is
processing specimens.
Retort #1 / #2 Reagent Cabinet Access Door ..6.. —
provides access to retort #1 / #2 Reagent Cabinet for the
purposes of replacing reagents.
Retort #3 / #4 Reagent Cabinet Access Door ..7.. —
provides access to the paraffin oven for the purposes of
replacing reagents.
Control Panel ..8.. — comprises the controls and
indicators necessary to program and operate the instru-
ment, and to monitor the instrument during processing
(refer to “Control Panel (Figure 1-O)” on page 1.12 for
details).
Fume Control System Access Door ..9.. — provides
access to the fume control system for purposes of
replacing the fume filter.
Overflow Bottle Access Door ..10.. — provides access
to retorts #3 / #4 overflow bottles for purposes of cleaning
and servicing.
Leveling Feet/Casters ..11.. — four casters are provided
to allow for easy repositioning of the instrument. Adjusta-
ble leveling feet associated with each caster facilitate
proper leveling of the instrument.
Cabinet Rear (Figure 1-B)
The power cord connector and external interface are
located at the rear of the back cabinet.
Figure 1-B
Power Cord Connector ..12.. — accepts the instrument
end of the power cord; opposite end of the power cord
may be connected to facility power.

Revised 10/02/2012 1.5
Figure 1-C
Power Switch ..13.. — Located on the right side panel of
the cabinet (Figure 1-C), turns the power on and off.
System Start Button ..14.. —A button located on the right
side panel above the power switch, (Figure 1-C) turns on
the computer system.
Internal Light Button ..15.. — Turns the light inside the
Tissue-Tek Xpress x120 on or off (Figure 1-D). The light
must be manually turned off. Shutting down the instru-
ment will not turn the light off.
USB Interface ..16. — USB communication port for
connection to a memory stick for transfer of data to an
external PC (Figure 1-D).
Figure 1-D
External Alarm Output Port — allows for connection of
an external audible alarm to a switched no-voltage contact
within the Tissue-Tek Xpress x120; contact is switched
when an alarm condition occurs.
UPS Signal Input Port — provides for connection of the
Tissue-Tek Xpress x120 to an external Uninterruptible
Power Supply (UPS) or backup generator.

INTRODUCTION
1.6 Revised 10/02/2012
Location of Major Systems and
Components
The Tissue-Tek Xpress x120 instrument is comprised of
the following major systems and components (Figure 1-E):
•Control Panel ..8..
•Loading Station ..17..
•Retort #1 ..18..
•Retort #2 ..19..
•Retort #3 ..20..
•Retort #4 ..21..
•Unloading Station ..22..
•Transfer System ..23..
•Fume Control System ..24..
•Reagent Cabinet ..25..
•Paraffin Oven ..26..
•Overflow Bottles ..27..
•Accessory Compartment ..28..
Major systems and components of the Tissue-Tek
Xpress x120 instrument are described in the following
paragraphs.
Figure 1-E

Revised 10/02/2012 1.7
Loading Station (Figure 1-F)
Figure 1-F
The Loading Station (Figure 1-F) is an unheated, rea-
gent-filled, removable stainless steel container ..17.. that
facilitates the loading of a single basket or two Paraform®
magazines, containing a maximum of 40 tissue cassettes,
into the Tissue-Tek Xpress x120. The Loading Station
retort holds a maximum of approximately 1.8 liters of
reagent (Preprocessing Solution, product code 7115).
Access to the Loading Station is facilitated by an ac-
cess door in the Cabinet Front (see “Cabinet Front (Figure
1-A)” on page 1.3 for details).
A removable lid protects the retort contents (reagent)
when the instrument is not in operation.
Microwave Stations (Retorts #1 and #2)
NOTE: Retort #1 and Retort #2 are identical.
At these Mircrowave Stations (Retorts #1 and #2)
(..18.., ..19.., Figure 1-G) dehydration and clearing
processes are performed. The reagent in the Microwave
Station retorts is heated by radiating microwaves. Each
Microwave Station is comprised essentially of a micro-
wave retort and associated microwave unit (microwave
applicator and power supply), preheating chamber,
reagent container, and reagent cabinet.
Microwave Retorts (Figure 1-G)
Figure 1-G
Each microwave retort supports the processing of a
single basket or two Paraform magazines containing a
maximum of 40 cassettes. Each microwave retort has a
designated reagent supply level of approximately 1.44
liters, with a maximum capacity of approximately 1.74
liters. Filing and draining of the retorts is accomplished by
a reagent flow circuit operating under software control.
The flow control circuit incorporates an interlock that
prevents reagent from draining out of the retort if a
reagent container is not in place in the reagent cabinet.
A two-level reagent level detector is provided. The
lower level is used to stop supplying reagent when
replenishing; the upper level is used to prevent reagent
overflow. When the upper level sensor is actuated, an
audible alarm sounds, and the line pump is stopped.
A software controlled temperature regulation unit al-
lows control of reagent temperature at 51°C. The set
temperature for the microwave retorts is established
during installation, according to elevation of the installation
site. A bubbling pump is employed to supply volatilized
reagent to the microwave retort to aid in equalizing the
temperature of the reagent within the retort.
A hinged lid protects the retort contents during pro-
cessing. The lid is opened automatically during transfer
operations, and may be opened manually when no power
is applied to the motor to allow for cleaning and mainte-
nance of the retort. Each retort lid has a sensor that
prevents the associated microwave unit from being
energized in the event the lid is not fully closed, and
immediately turn off the magnetron in the event the lid is
opened during processing.

INTRODUCTION
1.8 Revised 10/02/2012
Preheating Chambers
Preheating chambers are used to temporarily store
reagent when draining Retorts #1 and #2 prior to transfer-
ring a basket or magazines. The Preheating Chambers
are located between the retorts and the reagent contain-
ers, and keep the reagents warm during basket/magazine
transfers. No user access is required except when
cleaning the Preheating Chamber sight tubes (see
“Preheating Chamber Sight Tube” on page 7.11). Each
preheating chamber has a maximum capacity of approxi-
mately 2.4 liters.
A three-sensor level detection circuit is provided for
reagent level detection.
•The lower-level detector is used to stop supplying
reagent (after supplying approximately 0.6 to 0.8 liter)
when replenishing reagent in Retorts #1 and #2. The
sensor is also used to detect low reagent volume lev-
el for reagent replenishing.
•The mid-level sensor is used to prevent overflow from
the preheating chamber during reagent transfer from
the retort to the preheating chamber. When the mid-
level sensor is actuated, reagent is drained from the
retort to the reagent container rather than being trans-
ferred to the preheating chamber.
•The upper sensor is used to prevent reagent
overflow. The sensor is actuated at approximately 2.5
liters. When the upper sensor is actuated, an audible
alarm sounds, and the line pump is stopped.
A software-controlled temperature regulation unit main-
tains temperature in the preheating chamber.
Reagent Cabinet (Figure 1-H)
Figure 1-H
The reagent cabinet ..25.. is accessed through a door
in the cabinet front (see “Cabinet Front (Figure 1-A)” on
page 1.3 for details). The reagent cabinet provides
storage space for the reagent containers used by Retorts
#1 and #2. A two-position locking lever .25a.associated
with each Retort couples the associated reagent container
with the reagent flow circuit, and secures the container in
place in the cabinet. The reagent cabinet also interfaces
with the Tissue-Tek Xpress x120 fume control system
(see “Fume Control System” on page 1.11 for details).
The reagent containers .25b.are disposable containers
that hold the processing reagents for Retorts #1 and #2.
Each reagent container is equipped with a two-piece
cap/connector .25c.. The upper cap is used during
shipment and storage of the container. The lower cap
facilitates connection of the container to the Tissue-Tek
Xpress x120 system. The same reagent is used in Retorts
#1 and #2. The caps and labels are color-coded blue so
that the containers cannot be mistaken for other reagents.

Revised 10/02/2012 1.9
Vacuum Stations (Retorts #3 and #4)
NOTE: Retort #3 and Retort #4 are identical.
These Vacuum Stations (Retorts #3 and #4) (..20..,
..21.., Figure 1-I) are where the paraffin impregnation
processes are performed. The vacuum in the vacuum
retort increases removal of the Microwave Station
reagents from the tissue specimens, and improves
paraffin impregnation of the specimens. Each Vacuum
Station is comprised essentially of a vacuum retort with
integral retort heater, vacuum pump, paraffin oven, and
overflow bottles.
Vacuum Retorts (Figure 1-I)
Figure 1-I
Each vacuum retort supports the processing of a single
basket or two Paraform magazines containing a maximum
of 40 cassettes. Each vacuum retort has a maximum
reagent capacity of approximately 2.0 liters. Filling of the
retorts is accomplished by a vacuum pump and reagent
flow circuit operating under software control. An ultrasonic
sensor is provided for reagent level detection. The sensor
is used to stop supplying reagent (at approximately 1.7
liters), when replenishing. The retorts utilize gravity to
drain reagent to the paraffin oven.
A software-controlled temperature regulation allows
control of reagent temperature at 65°C. An over tempera-
ture control (thermal fuse) prevents overheating of the
reagent.
A hinged lid protects the retort contents during pro-
cessing. The lid is opened automatically during transfer
operations, and may be opened manually when no power
is applied to the motor to allow for cleaning and mainte-
nance of the retort.
Vacuum pumps are used to pump reagent (melted
paraffin) into the Vacuum Station retorts. The vacuum
pump for Vacuum Retorts applies vacuum for 80 seconds,
and then is off for 10 seconds (no vacuum applied). This
cycle is repeated continuously.
Reagent is drained from the Vacuum Station retorts to
the paraffin oven by gravity. Drain time is approximately
five minutes.
Paraffin Oven (Figure 1-J)
Figure 1-J
The paraffin oven ..26.. is accessed through a door in
the cabinet front (see “Cabinet Front (Figure 1-A)” on page
1.3 for details). The paraffin oven is a two-compartment,
drawer-mounted container that provides for the storage
and heating (melting) of the Vacuum Station reagents.
The oven has the capability to melt paraffin at a rate of 2.4
kg (3.0 liters) within 4 hours. Temperature of the paraffin
oven is controlled at the set temperature of Vacuum
Stations Retorts #3 and #4 by a software-controlled
temperature regulation unit. An over temperature control
(thermal fuse) prevents overheating of the reagent.
The oven incorporates separate compartments for
Vacuum Station Retort #3 and Vacuum Station Retort #4
reagents. Each compartment has a capacity of approxi-
mately 3.0 liters of paraffin chips. A reagent level mark in
each compartment indicates the proper reagent level
inside the compartment (approximately 3.0 liters).

INTRODUCTION
1.10 Revised 10/02/2012
Figure 1-J
The oven compartments are not removable, but slide in
and out of the Tissue-Tek Xpress x120 cabinet for
purposes of filling, draining, and maintenance. A spigot
facilitates the draining of the reagents (see “Retort #3 and
#4 Paraffin Replacement” on page 7.5). Each compart-
ment is covered by a protective hinged lid ..26a... A drip
pan ..26b.. is provided at the front of the paraffin oven to
prevent drips of melted paraffin from falling to the floor
around the unit that may occur during the filling or
maintenance of the oven.
Overflow Bottles (Figure 1-K)
Figure 1-K
An overflow bottle ..27.. associated with each Vacuum
Retort serves as an overflow receptacle in the event
excess reagent (paraffin) is pumped into the associated
retort. Each bottle has a capacity of 250 milliliters. A
proximity sensor ..27a.. (located behind each overflow
bottle) is provided for reagent level detection. The
overflow bottles are accessed through a door in the
cabinet front (see “Cabinet Front (Figure 1-A)” on page 1.3
for details).
Unloading Station (Figure 1-L)
The Unloading Station provides for storage of baskets
or magazine sets after completing tissue processing.
Access to the Unloading Station is facilitated by an access
door in the Cabinet Front (see “Cabinet Front (Figure 1-A)”
on page 1.3 for details). The Unloading Station is
comprised essentially of a dual-retort, heater, and
transport tray.
Figure 1-L
The dual-retort ..22.. allows for temporary storage of
two processed baskets or four Paraform magazines
containing a maximum of 80 tissue cassettes. The retort is
removable for ease in cleaning. A software-controlled
temperature regulation unit maintains the paraffin in the
processed specimens in a molten state prior to transport
and paraffin imbedding.
The heater allows control of retort temperature at 65°C.
An over temperature control prevents overheating.
A hinged lid protects the contents of the retorts during
storage. The lid is opened automatically during transfer
operations, and may be opened manually when no power
is applied to the motor to allow for cleaning and mainte-
nance of the retort.
A transport tray is provided to prevent paraffin dripping
when removing and manually transporting baskets from
the Tissue-Tek Xpress x120 to a paraffin embedding
center. The transport tray facilitates transport of two
baskets or four Paraform magazines simultaneously.

Revised 10/02/2012 1.11
Transfer System (Figure 1-M)
The Transfer System performs the automated process
of transferring baskets from the Loading Station to the
Microwave and Vacuum Stations, and ultimately to the
Unloading Station. The Transfer System is comprised of a
motor-driven transfer arm ..23.. that provides for move-
ment in the X (horizontal) and Z (vertical) axes.
Figure 1-M
When the instrument is not processing specimens, the
transfer arm can be moved manually.
A drip pan ..23a.. associated with the transfer arm
prevents reagent from dripping onto the top panel in the
instrument when a basket is transferred between stations.
A disposal tray liner ..23b.. receives reagent drips. A
rotational drive associated with the drip pan automatically
retracts the drip pan to allow the transfer arm to raise or
lower a basket. The drip pan is returned to its location
beneath the basket while traveling horizontally between
stations.
Fume Control System (Figure 1-N)
Figure 1-N
The Fume Control System ..24.. is comprised essential-
ly of a hood, activated carbon absorption filter, and
exhaust fan. The Fume Control System is accessed
through a door in the cabinet front (see “Cabinet Front
(Figure 1-A)” on page 1.3 for details).
The Fume Control System hood serves to prevent
hazardous fumes from leaking to the outside of the
instrument. The system collects reagent fumes from inside
the instrument and passes them through an activated
carbon absorption filter to reduce fume emissions to
acceptable levels. The filtered air is then discharged to the
atmosphere. The fume control exhaust fan is active
whenever power is applied to the instrument. In the event
the fume control exhaust fan fails while the instrument is in
operation (processing is being performed), an audible
alarm sounds.
Additionally, a duct is provided on the instrument rear
panel to facilitate connection of the Fume Control System
to a facility exhaust system.

INTRODUCTION
1.12 Revised 10/02/2012
Control Panel (Figure 1-O)
The Control Panel ..8.. provides the operating controls
and indicators necessary to operate the instrument,
monitor system operation, configure system settings, and
view status and historical reports.
Figure 1-O
Main Power Indicator ..8a.. — lights when main power is
applied to the instrument.
System Status Indicator ..8b.. — provides an indication
of the current status of the system. Flashes when the
WARMING UP SYSTEM mode is in process. Lights
steady on when the system is ready for processing.
Auto Start / Shutdown Indicator ..8c.. — indicates the
Auto Start/Shutdown function is activated (see “Section 3,
Setting Ready/Shutdown Times” on page 3.7).
LCD Display ..8d.. — 800 X 600 pixel touch screen LCD
display. The instrument sounds a tone whenever a key,
button or text field is actuated (with the exception of the
10-key keypad and alpha keyboard). When in processing
mode, provides the data entry and display screens to login
to the system, prepare a basket for processing, and
monitor processing status (see “Section 4, Operating
Instructions” for details). When in utility mode, provides
the data entry and display screens to manage instrument
users, configure system settings, and view statistical data
(see “Section 3, Customization of Settings” and “Section
4, Operating Instructions” for details).
Specifications
Models Covered By This Manual:
Product
Code Name/Description
7720 Tissue-Tek®Xpress®x120 Continuous Rapid
Tissue Processor (200 VAC) (USA)
7721 Tissue-Tek®Xpress®x120
Continuous Rapid
Tissue Processor (200 VAC) (Asia)
7722 Tissue-Tek®Xpress®x120 Continuous Rapid
Tissue Processor (230 VAC) (Europe)
Power Requirements:
Model 7720 – 200VAC ±10%, 50/60Hz, single-phase,
20 amps
Model 7721 – 200VAC ±10%, 50/60Hz, single-phase,
20 amps
Model 7722 – 230VAC ±10%, 50/60Hz, single-phase,
20 amps
Power Ratings:
Model 7720 – 200VAC, 50/60Hz, single-phase, 15 amps
Model 7721 – 200VAC, 50/60Hz, single-phase, 15 amps
Model 7722 – 230VAC, 50/60Hz, single-phase, 13 amps
Noise Level: Less than 65 db
Hazardous Fume Control
Complies with the following requirements for acetone as
fume emissions in laboratories:
1,000 ppm, Occupational Safety and Health Admin-
istration (USA); 750 ppm, Industrial Safety and Health
Law (Japan); 500 ppm, Maximale Arbeitsplatz-
Konzentration (Europe)
Environmental Requirements:
Operating:
Temperature Range: +15°C to +35°C
Relative Humidity: 30% to 85%, non-condensing
Relative Atmospheric Pressure: 70 to 106 kPa (525 to
795 mm Hg)
Pollution Degree: 2

Revised 10/02/2012 1.13
Storage:
Temperature Range:-10°C to +65°C
Relative Humidity: 30% to 95%, non-condensing
Relative Atmospheric Pressure: 70 to 106 kPa (525 to
795 mmHg)
Dimensions:
Height: 162 cm (63.8 in.)
Width: 170 cm (66.9 in.)
Depth: 70 cm (27.6 in.)
Weight: 465 kg (1023 lbs)
Instrument Life Expectancy: 7 years (with
instrument powered on 24 hours a day, every day)
Safety Standards
Electrical Safety:
For Model 7720 –
cETL Certification
Complies with:
UL 61010-1: 2nd Ed.: 2004,
CAN/CSA-C22.2 No. 61010-1: 2nd Ed.: 2004,
CAN/CSA-C22.2 No. 61010-2-010:04,
IEC61010-2-010: 2003
CAN/CSA-C22.2 No. 61010-2-081:04,
IEC61010-2-081: 2001
CAN/CSA-C22.2 No. 61010-2-101: 04,
IEC61010-2-101: 2002
FDA Registration (Regulation No. 864.3875)
For Model 7721 –
Japanese Pharmaceutical Law
Complies with:
Quality Management System- ISO 13485: 2003
Electrical Safety
JIS C 1010-1: 2005
IEC61010: 2001 (ed2)
IEC61010-2: 010: 2003
IEC61010-2-081: 2001
IEC61010-2-101: 2002
EMC- JIS C1806-1: 2001
Risk Management- JIS T 14971: 2003
For Model 7722 –
Complies with:
LVD, IVD
EN61010-1: 2001 (ed2)
EN61010-2-010: 2003 (ed2)
EN61010-2-081: 2001
EN61010-2-101: 2002
EMC
CISPR11: 1999 Group 1 Class A (EN55011)
EN50082-1: 1997
EN61000-3-3: 1994 Amendment 1&2 (2001)
EN61000-4-2: 1995 Amendment 1&2 (2001)
EN61000-4-3: 2002
EN61000-4-4: 2004
EN61000-4-5: 1995 Amendment 1
EN61000-4-6: 1996
Amendement1&2&3&correction1
EN61000-4-11: 2004
Regulatory Information:
FDA Certified with ETL evaluation (USA); complies
with requirements of The Pharmaceutical Affaires
Law (Japan); CE Certified in compliance with the re-
quirements of EMC Directive, LV Directive in Europe
(Europe)

INTRODUCTION
1.14 Revised 10/02/2012