SCIEX CESI 8000 Plus User manual

CESI 8000 Plus High Performance
Separation-ESI Module
Preinstallation Manual
August 2016RUO-IDV-05-3345-B | B11953AE

This document is provided to customers who have purchased SCIEX equipment to use in the operation of such SCIEX
equipment. This document is copyright protected and any reproduction of this document or any part of this document is
strictly prohibited, except as SCIEX may authorize in writing.
Software that may be described in this document is furnished under a license agreement. It is against the law to copy, modify,
or distribute the software on any medium, except as specifically allowed in the license agreement. Furthermore, the license
agreement may prohibit the software from being disassembled, reverse engineered, or decompiled for any purpose. Warranties
are as stated therein.
Portions of this document may make reference to other manufacturers and/or their products, which may contain parts whose
names are registered as trademarks and/or function as trademarks of their respective owners. Any such use is intended only
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any right and/or license to use or permit others to use such manufacturers' and/or their product names as trademarks.
SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are SCIEX’s
sole and exclusive representations, warranties, and obligations. SCIEX makes no other warranty of any kind whatsoever,
expressed or implied, including without limitation, warranties of merchantability or fitness for a particular purpose, whether
arising from a statute or otherwise in law or from a course of dealing or usage of trade, all of which are expressly disclaimed,
and assumes no responsibility or contingent liability, including indirect or consequential damages, for any use by the purchaser
or for any adverse circumstances arising therefrom.
For research use only. Not for use in diagnostic procedures.
AB Sciex is doing business as SCIEX.
The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™is being used under license.
©2016 AB Sciex
AB Sciex LLC
500 Old Connecticut Path
Framingham, Massachusetts 01701
USA
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Chapter 1 Introduction...............................................................................................................................5
Customer Site Planner Responsibilities..............................................................................................................................5
FSE Responsibilities............................................................................................................................................................6
During Installation.......................................................................................................................................................6
Operator Familiarization..............................................................................................................................................6
Technical Support..............................................................................................................................................................6
Chapter 2 Site Planning Checklist..............................................................................................................7
Customer Information........................................................................................................................................................7
Requirements.....................................................................................................................................................................7
Site Layout...................................................................................................................................................................7
Electrical Requirements................................................................................................................................................8
Ventilation and Waste Collection Requirements..........................................................................................................8
Environmental Requirements.......................................................................................................................................8
BioSafety Requirements...............................................................................................................................................9
Customer Preparation..................................................................................................................................................9
Mass Spectrometer Requirements................................................................................................................................9
Solutions and Equipment Requirements......................................................................................................................9
Mass Spectrometer Information.......................................................................................................................................10
Customer Profile..............................................................................................................................................................10
Comments and Exceptions...............................................................................................................................................11
Signoff.............................................................................................................................................................................11
Appendix A Site Requirements................................................................................................................12
Site Layout Requirements................................................................................................................................................12
Laboratory Layout and Clearances.............................................................................................................................12
Height Requirements.................................................................................................................................................13
Weights and Dimensions...........................................................................................................................................13
Electrical Requirements....................................................................................................................................................14
Mains Supply Connections.........................................................................................................................................14
Mains Supply Fluctuations.........................................................................................................................................14
Protective Earth Conductor........................................................................................................................................14
Surge Suppression......................................................................................................................................................14
System Electrical Specifications..................................................................................................................................14
Ventilation and Waste Collection Requirements..............................................................................................................15
Ventilation Requirements...........................................................................................................................................15
Waste Collection Requirements.................................................................................................................................15
Environmental Requirements...........................................................................................................................................15
BioSafety Requirements.............................................................................................................................................16
Customer Preparation Requirements...............................................................................................................................16
Mass Spectrometer Requirements....................................................................................................................................16
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Preinstallation Manual
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Contents

SCIEX Mass Spectrometers........................................................................................................................................16
Thermo Scientific Mass Spectrometers.......................................................................................................................17
Bruker Mass Spectrometers.......................................................................................................................................17
Waters Mass Spectrometers.......................................................................................................................................17
Customer-Supplied Solutions and Equipment..................................................................................................................17
Appendix B Equipment Safety Categories..............................................................................................19
Appendix C System Specifications...........................................................................................................20
Instrument Specifications.................................................................................................................................................20
Cart Specifications...........................................................................................................................................................20
Controller Specifications..................................................................................................................................................21
Detector Specifications....................................................................................................................................................21
(Optional) UV Detector Specifications........................................................................................................................21
(Optional) Laser Induced Fluorescence (LIF) Detector Specifications..........................................................................22
(Optional) Photo Diode Array (PDA) Detector Specifications......................................................................................22
Revision History........................................................................................................................................24
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Contents

This guide is for the site planner, the individual responsible for preparing the facility for the installation of the CESI
8000 Plus system.
Note: Refer to the Safety Manual for instructions for safe use of the system.
Note: If the site preparation tasks are not complete when the SCIEX Field Service Employee (FSE) arrives, then
the scheduled installation will be postponed.
Customer Site Planner Responsibilities
Complete the Site Planning Checklist on page 7 in consultation with facilities services personnel (electrical,
ventilation, and information technology [IT]), and return it to the FSE before the completion date. Refer to Signoff
on page 11.
Note: The FSE will follow up if the checklist is not received prior to the scheduled installation date.
•Verify that adequate space and the required shipping facilities are available. Refer to Site Layout Requirements
on page 12.
•Provide all required electrical receptacles. Refer to Electrical Requirements on page 14.
•Provide and install all required vents and ventilation devices. Refer to Ventilation and Waste Collection
RequirementsVentilation Requirements on page 15.
•Verify that the requirements for the operating environment are met. Refer to Environmental Requirements on
page 15.
•Verify the mass spectrometer to be used with the system is operational by executing a performance test. Refer
to Mass Spectrometer Requirements on page 16.
•Verify that any required additional equipment for the ion source or mass spectrometer is present. Refer to Mass
Spectrometer Requirements on page 16.
•Verify that all required solvents and laboratory supplies are available. Refer to Customer-Supplied Solutions
and Equipment on page 17.
•When the shipment arrives, inspect the packaging for damage and then contact SCIEX Customer Service or
the local FSE to schedule the installation.
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1
Introduction

FSE Responsibilities
•Review the checklist and discuss outstanding issues with the site planner.
•Unpack and set up the CE equipment sold and supported by SCIEX.
•Test and qualify the system to the specifications in the Operational Qualification 1.
During Installation
WARNING! Lifting Hazard. Make sure that at least four people are available to lift
the CE system. Follow established safe lifting procedures.
The FSE unpacks the system (with the assistance of customer staff), sets up the system, and then confirms its
operation. When the system is installed, the FSE conducts an operational qualification.
Operator Familiarization
During installation, the FSE provides a system and software overview, reviews data, and provides some basic
operator familiarization.
Note: Online training is available at sciex.com/education .
Technical Support
SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughout
the world. They can answer questions about the system or any technical issues that might arise. For more
information, visit the SCIEX Web site at sciex.com.
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Introduction

Customer Information
Organization
Address
City
Country
Zip/Postal code
Telephone
FaxSite planner contact
name
E-mail address
Requirements
Site Layout
Refer to Site Layout Requirements on page 12.
CompleteRequirements
There is adequate lab space to accommodate the equipment.
If the mass spectrometer inlet is lower or higher than the range of the CESI 8000 Plus cart, a
table or stand of the appropriate height for the CESI 8000 Plus system is available.
Additional personnel are available to help the FSE move the equipment. Four people are
recommended.
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2
Site Planning Checklist

Electrical Requirements
Refer to Electrical Requirements on page 14.
N/ACompleteRequirement
Installation of electrical supplies and fixtures complies with local regulations and
safety standards.
One branch circuit is provided for the CE components. One AC mains supply outlet
is required.
The mains supply voltage does not fluctuate more than ±10% from the nominal
voltage.
The mains supply includes a correctly installed protective earth conductor.
A qualified electrician has determined the appropriate AC mains supply
configuration based on the system electrical specifications found in System
Electrical Specifications on page 14.
Ventilation and Waste Collection Requirements
Refer to Ventilation and Waste Collection Requirements on page 15.
CompleteRequirement
Ventilation of the laboratory environment in which the system will be used complies with
local regulations and the air exchange rate is appropriate for the work performed.
Environmental Requirements
Refer to Environmental Requirements on page 15.
CompleteRequirement
The altitude does not exceed 2000 m (6562 ft) above sea level.
Temperature and humidity requirements have been met.
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Site Planning Checklist

BioSafety Requirements
Refer to BioSafety Requirements on page 16.
CompleteRequirement
The site is not designated as BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4).
Customer Preparation
Refer to Customer Preparation Requirements on page 16.
CompleteRequirement
Up to two customers have been identified for familiarization.
A mass spectrometer will be available during the installation.
Mass Spectrometer Requirements
Refer to Mass Spectrometer Requirements on page 16.
CompleteRequirement
A performance test has been performed on the mass spectrometer and a printout of the results
is attached.
Any required equipment for the ion source or mass spectrometer is available at the site.
Solutions and Equipment Requirements
Refer to Customer-Supplied Solutions and Equipment on page 17.
N/ACompleteRequirement
All of the required solutions and bottles are available.
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Site Planning Checklist

Mass Spectrometer Information
Mass spectrometer
manufacturer
Mass spectrometer model
Mass spectrometer serial
number
Software version
Firmware version
Ion source model
Customer Profile
(Optional) To allow the FSE to provide site-specific training, enter the following information.
Number of operators
Years of experience with mass spectrometry
Years of experience with capillary electrophoresis
CESI applications to be performed
Will the CESI 8000 Plus system be used stand-alone?
If the system will be used stand-alone, which detectors will be used?
UV
LIFPDA
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Site Planning Checklist

Comments and Exceptions
Signoff
Completion date
(yyyy-mm-dd)
Site planner contact name
I acknowledge that all of the installation requirements, as specified in this document, have
been met.
Return date
(yyyy-mm-dd)
FSE name
FSE e-mail
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Site Planning Checklist

Site Layout Requirements
Laboratory Layout and Clearances
Return to checklist.
•The floor is smooth and level so that the mobile cart does not roll.
•The location allows at least 10.2 cm (4 inches) on all sides of the system.
•The location is away from heaters or cooling ducts, and not in direct sunlight.
•The location is away from vibrating equipment, such as refrigerators or centrifuges.
•For configurations where the CESI 8000 Plus controller is connected to the mass spectrometer acquisition
computer with a 4.3 m (14 feet) cable, the maximum distance between the controller and the acquisition
computer is 4 m (13 feet).
Figure A-1 Suggested Laboratory Layout
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A
Site Requirements

DescriptionItem
Distance to wall, minimum 10.2 cm (4 inches)1
Mains supply2
Surge suppressor3
CESI 8000 Plus cart with controller and CESI 8000 Plus system4
Distance determined at installation, approximately 10 cm (4 inches) (see note below)5
Mass spectrometer6
Height Requirements
The alignment mark on the outside of the CESI 8000 Plus system is 36 cm (14 inches) from the bottom of the
system. This mark must be vertically aligned with the mass spectrometer inlet. To align the two systems, the height
of the cart provided with the CESI 8000 Plus system is adjustable.
The cart can be used with mass spectrometers with inlets that are located between 105 cm (41 inches) and 148
cm (58 inches) from the floor. If the height of the inlet is outside of this range, the customer must provide a table
or stand for the CESI 8000 Plus system.
Weights and Dimensions
Refer to the following table for weights and dimensions and make sure that the system can be moved to the
installation site. Make sure that the installation site can accommodate the equipment dimensions, weight, and
associated clearance.
WeightDepthWidthHeightEquipment
85.3 kg
(188 lbs)
72.4 cm
(28.5 inches)
63.5 cm
(25 inches)
99.1 cm (39 inches) (cover open)
73.1 cm (29 inches) (cover closed)
CESI 8000 Plus system
69.0 kg
(152 lbs)
73.7 cm
(29 inches)
9.1 cm
(36 inches)
66.6 cm to 111.8 cm
(27 inches to 44 inches)
CESI 8000 Plus cart
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Site Requirements

Electrical Requirements
Return to checklist.
WARNING! Electrical Shock Hazard. Use only qualified personnel for the installation
of all of the electrical supplies and fixtures, and make sure that all of the installations
adhere to local regulations and safety standards.
Mains Supply Connections
WARNING! Electrical Shock Hazard. Make sure that the system can be disconnected
from the mains supply outlet in an emergency. Do not block the mains supply outlet.
Mains Supply Fluctuations
In areas where the mains supply is subject to voltage fluctuations exceeding ±10% of the nominal value (100 VAC
to 240 VAC), a power conditioner is required. High or low voltages can adversely affect the electronic components
of the CE system.
Protective Earth Conductor
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the protective
earth conductor. Any interruption of the protective earth conductor creates an
electrical shock hazard.
The mains supply must include a correctly installed protective earth conductor that must be installed or inspected
by a qualified electrician before the system is connected.
Surge Suppression
The CESI 8000 Plus system must be plugged into a high capacity electrical surge suppressor, to protect the system
from voltage spikes and power surges. The maximum current is the sum of the maximum current ratings for all
components.
System Electrical Specifications
Note: Specifications are subject to change without notice.
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Site Requirements

Table A-1 CESI 8000 Plus Module Electrical Specifications
ValueSpecification
Instrument: 100 VAC to 240 VAC
Cart: 100 VAC to 240 VAC
Nominal input voltage
50 Hz or 60 HzFrequency
Instrument: 5 AMaximum input current
Power Consumption
Supply voltage must not exceed 10% of nominal.
Ventilation and Waste Collection Requirements
Return to checklist.
Ventilation Requirements
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Be sure
to use the system in a well-ventilated laboratory environment in compliance
with local regulations and with appropriate air exchange for the work
performed.
Waste Collection Requirements
WARNING! Biohazard, Toxic Chemical Hazard. Follow local directives when
disposing of chemicals, vials and caps, and the remains of the prepared
samples, if applicable. They might contain regulated compounds and
biohazardous agents.
Environmental Requirements
•Altitude not exceeding 2000 m (6562 feet) above sea level
•An ambient temperature of 15 °C to 30 °C (59 °F to 86 °F)
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Site Requirements

•Relative humidity from 20% to 60%, non-condensing
BioSafety Requirements
The site must not be designated BioSafety Level 3 (BSL-3) or BioSafety Level 4 (BSL-4). SCIEX does not install,
service, or repair SCIEX systems in areas designated BSL-3 or BSL-4.
Customer Preparation Requirements
Return to checklist.
After the system has been installed, an FSE will work with up to two operators in basic system operation.
Operators should:
•Know how to operate the mass spectrometer and be willing to operate it during the installation.
•Be available for the entire training period.
Mass Spectrometer Requirements
Return to checklist.
Before the CESI 8000 Plus system can be installed, the mass spectrometer must be operational. To demonstrate
this, a performance test should be performed and a printout of the results attached to this document.
For some mass spectrometers, additional equipment is required, refer to the appropriate section below.
SCIEX Mass Spectrometers
For the OptiMS Adapter for SCIEX NanoSpray III source (PN B07363), all systems require the NanoSpray®III ion
source retrofit kit (PN 5030793), purchased from SCIEX.
Make sure that the required orifice plate for the NanoSpray®ion source is present (refer to the following table).
Orifice Plate
Part Number
NanoSpray®III Ion
Source Part Number
Model
10337315028129SCIEX Triple QuadTM 5500 system
QTRAP®5500 system
TripleTOF®4600, 5600, and 5600+ systems
10337315029154TripleTOF®6600 system
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Site Requirements

Orifice Plate
Part Number
NanoSpray®III Ion
Source Part Number
Model
50189505028138SCIEX Triple QuadTM 6500 and 6500+ systems
QTRAP®6500 and 6500+ systems
Thermo Scientific Mass Spectrometers
For the OptiMS Adapter for Thermo Nanospray II MS Sources (PN B07366), make sure the additional required
equipment is present (refer to the following table).
Additional Required EquipmentIon SourceModel
Ion MAX adapterNanospray II ion sourceLCQ Deca XP MAX, LTQ series and the TSQ
series systems
Adapter ringNanospray II ion sourceLCQ Deca XP and LCQ Advantage XP systems
For the OptiMS Thermo MS Adapter for Nanospray Flex and Nanospray Flex NG Sources (PN B83386), no additional
equipment is required.
Bruker Mass Spectrometers
The OptiMS Bruker MS Adapter for Bruker Mass Spectrometers (PN B86099) requires the NanoElectrospray capillary
cap set (PN 212915), purchased from Bruker.
For Bruker mass spectrometers that are close to the ground, the CESI 8000 Plus system requires a low table or
stand (refer to Height Requirements on page 13).
Waters Mass Spectrometers
No additional equipment from Waters is required for the OptiMS Waters MS Adapter for NanoLockSpray and
NanoFlow Ion Sources (PN B85211).
Customer-Supplied Solutions and Equipment
Return to checklist.
WARNING! Toxic Chemical Hazard. Refer to the chemical product Safety Data Sheets
and follow all of the recommended safety procedures when handling, storing, and
disposing of chemicals.
General Equipment
•Powder-free gloves (neoprene or nitrile recommended)
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Site Requirements

•Lint-free wipes
•Safety glasses
•Laboratory coat
•Pipettors (2 μL, 10 μL, 20 μL, 100 or 200 μL, and 1 mL) and appropriate tips
•pH meter
•Benchtop centrifuge
Glassware and Consumables
•Glass bottles (20 mL, 100 mL)
•20 mL glass vial
•0.5 mL centrifuge tube
•50 mL volumetric flask
Reagents
•1.0 N NaOH (Sigma PN 319511 or equivalent)
•0.1 N HCl (Sigma PN 84428 or equivalent)
•0.1 N NaOH (PN 338424 or equivalent)
•7.5 M ammonium acetate (Sigma PN A2706)
•Glacial acetic acid (Sigma PN A6283 or equivalent)
•Methanol (Fisher PN A454 or equivalent)
•Double-deionized (DDI) water (MS grade water filtered through 0.2 μm filter and with resistance above 18
megaohms)
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Site Requirements

CategoryDescription
Pollution Degree 2Equipment pollution degree
Note: Environments with a Pollution Degree 2 rating include laboratories and sales and commercial areas.
For more information, refer to the International Electrotechnical Commission standards IEC 61010-1 and IEC 60364.
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B
Equipment Safety Categories

Instrument Specifications
99.1 cm (cover open), 73.7 cm (cover closed) × 63.5 cm × 72.4 cm
(39 inches (cover open), 29 inches (cover closed) × 25 inches × 28.5 inches)
Dimensions (H × W × D)
85.3 kg (188 pounds)Weight
Power requirement: 100 VAC to 240 VAC, 5.0 A, 50 Hz or 60 Hz
Power consumption: supply voltage must not exceed 10% of nominal
Fuses:
•8.0 A slow blow; 1/4 inch (2 ea.); 100 VAC to 120 VAC
•6.3 A time delay; 20 mm (2 ea.); 200 VAC to 240 VAC
Installation (overvoltage) category: Category II
Electrical
TTL: 2
Contact closures: 2
I/O
Altitude: 2000 m (6562 feet) above sea level
Humidity: 20% to 60%, non-condensing
Temperature: 15 °C to 30 °C (59 °F to 86 °F)
Working environment
400 Watts (1024 BTU/hr) , under steady-state conditionsMaximum heat dissipation
<70 dBMaximum sound pressure
Cart Specifications
91.4 cm × 73.7 cm (36 inches × 29 inches)Dimensions (W × D)
Adjustable from 68.6 cm to 111.8 cm (27 inches to 44 inches)Height
69.0 kg (152 pounds)Weight
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C
System Specifications