Secomam UVIKON XL User manual

UVIKON XL
UVIKON XS
Operating manual
Ref. 0M7717B

SUMMARY
1. REMARKS.....................................................................................................................................................3
2. WARRANTY.................................................................................................................................................5
3. INFORMATION...........................................................................................................................................7
4. PRECAUTION OF USE...............................................................................................................................9
4.1. GENERAL WARNINGS........................................................................................................................9
4.2. DESCRIPTION OF SYMBOLS............................................................................................................10
4.3. QUALITY, RELIABILITY AND SAFETY..........................................................................................10
5. INSTRUMENT DESCRIPTION ...............................................................................................................13
5.1. GENERAL POINTS..............................................................................................................................13
5.2. SPECIFICATIONS ...............................................................................................................................14
5.3. SYSTEM CONCEPT ............................................................................................................................15
5.3.1. Display...........................................................................................................................................15
5.3.2. Electronics.....................................................................................................................................15
5.3.3. Optics.............................................................................................................................................15
6. INSTALLATION ........................................................................................................................................17
6.1. UNPACKING AND INSTRUMENT SETUP.......................................................................................17
6.1.1. Unpacking......................................................................................................................................17
6.1.2. Checking the serial number of the instrument...............................................................................18
6.2. INSTALLING THE INSTRUMENT ....................................................................................................19
6.2.1. Environmental conditions..............................................................................................................19
6.3. INITIAL INSTALLATION...................................................................................................................20
6.3.1. Connexion of the power supply......................................................................................................20
6.3.2. Connection to the Personal Computer...........................................................................................20
6.3.2.1. PC minimum requirements.........................................................................................................................20
6.3.3. Software Installation, LabPower JUNIOR....................................................................................20
7. OPERATING INSTRUCTIONS................................................................................................................21
8. MAINTENANCE ........................................................................................................................................23
8.1. DECONTAMINATION........................................................................................................................23
8.1.1. Requirements.................................................................................................................................23
8.1.2. Risks...............................................................................................................................................23
8.2. CLEANING THE INSTRUMENT........................................................................................................24
9. OPTIONS AND ACCESSORIES ..............................................................................................................25
9.1. INTERNAL ACCESSORIES................................................................................................................25
9.2. EXTERNAL ACCESSORIES...............................................................................................................27
9.3. ACCESSORY CONFIGURATIONS....................................................................................................27

1. REMARKS
The Automatic aspiration system kit is manufactured by AQUALABO ANALYSE.
The information contained in this document may be the object of patents or patent applications by
AQUALABO ANALYSE. The possession of this document does not confer any license to such
patents.
The following names are AQUALABO ANALYSE’s registered trademarks throughout the world.
SECOMAM
UVIKON XL, UVIKON XS
This manual is updated periodically. The updates are included in the new editions.
All information supplied in this edition of the manual may be amended before the products described
herein are available.
All reproduction rights reserved.

2. WARRANTY
The new equipment and material sold by AQUALABO ANALYSE is guaranteed against any
manufacturing defects for one year (unless otherwise stated by AQUALABO ANALYSE) with effect:
−From the technical acceptance of the equipment in the factory by the buyer or his designee,
−or failing this :
* For Metropolitan France: from the date on the delivery note.
* For other destinations: from the date of factory shipment certified by air waybill,
consignment note or bill of lading.
The AQUALABO ANALYSE company guarantee applies exclusively to defectiveness arising from a
design fault or from a concealed defect. It is strictly limited to the free dispatching of replacement parts
(except for consumable items) or to the repairing of the equipment in our workshops within a deadline
of 10 working days (shipping delay not included).
By express agreement, the following are strictly excluded from our guarantee:
−All damages, notably for staff costs, loss of earnings, business trouble, etc
−Any breakdown due to an incorrect use of the equipment (non adapted mains, fall, attempt at
transformation, etc) or to a lack of maintenance by the user or to poor storage conditions.
−Any breakdown due to the use of parts not supplied by AQUALABO ANALYSE, on AQUALABO
ANALYSE equipment
−Any breakdown due to the transporting of the equipment in packaging which is not its original
packaging
−The lamps, the cells and generally any item which appears in the "accessories" section on the
price list.
Our customers are kindly asked to apply for our consent before returning any instrument for repair. No
return of materials may be accepted without the prior written consent of our Servicing after Sales
Management which will precise the terms of such return.
If the above consent is given, articles shall be returned in their original packaging on a prepaid basis to
the following address:
AQUALABO ANALYSE – USINE SECOMAM - 91 avenue des Pins d’Alep - 30100 ALES - France
We reserve the right to reship all instruments received collect failing such consent.
Whatever method and conditions of transport are chosen for the shipment of the equipment to be
repaired under guarantee, in the original packaging, the corresponding costs and the insurance costs
will be payable by the customer.
Any damage connected to the return transport of the equipment falls within the framework of the
guarantee on the express condition that the customer has sent his complaint within forty-eight hours
by registered letter with acknowledgement of receipt to the carrier. A copy of the letter should be sent
to AQUALABO ANALYSE.
For equipment with a guaranty card, this is only applicable if the card delivered with the equipment is
returned to AQUALABO ANALYSE duly completed.

UVIKON XL/XS
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SOFTWARE GUARANTEE
The software is guaranteed by the designer or the distributor of the software under the conditions
specified in the literature accompanying the aforementioned software packages.
Under no circumstances whatsoever will AQUALABO ANALYSE supply any type of guarantee for
software packages.
By express agreement, all damages, notably for staff costs, lost of earnings; business trouble, etc are
strictly excluded from our guarantee.
The customer is informed that the software cannot be guaranteed exempt from defects or bugs.
TRADE SECRET AND PROPERTY RIGHTS
This document is protected by a AQUALABO ANALYSE copyright (c) 2003 and the copying rights are
explicitly reserved. The software supplied with the equipment or referenced contains trade secrets and
confidential information which are AQUALABO ANALYSE's property. It is legally protected by the
international copyright (c) laws.
AQUALABO ANALYSE grants a license to use its software to the user. This may not be disclosed,
used or duplicated with the intention to save it, without AQUALABO ANALYSE's written permission.
The beneficiary must attach a copy of this document to all authorized partial or total reproductions.

3. INFORMATION
The AQUALABO ANALYSE - SECOMAM equipment has been designed, manufactured, tested and
inspected according to the ISO 9001 standards (Version 2000).
If the unit is not immediately installed, it should be stored in a dry and clean area. The storage
temperature should be between 10 and 35°C.
AQUALABO ANALYSE – SECOMAM equipment is carefully inspected before it is packed. As soon as
you receive your equipment, check the condition of the packaging and if you notice any problems,
notify your carrier within 48 hours. Then consult the packing list and check that everything is in order.
Finally, if you discover that something is missing, or if the goods are damaged immediately notify :
AQUALABO ANALYSE
USINE SECOMAM
91, Avenue des Pins d’Alep
30100 ALES
FRANCE
Tél. : [phone]
Fax : [phone]
E-mail : [e-mail]
Web: www.secomam.fr

4. PRECAUTION OF USE
The user should, before using this equipment, have read and understood this operating
manual, particularly the general warnings given below
4.1. GENERAL WARNINGS
Always disconnect the mains plug before starting any work inside the
instrument
Any maintenance operation inside the instrument, including lamp
replacement, must be carried out by a service engineer or trained
personnel authorised by SECOMAM. The user is permitted
access only to the inside of the sample compartment
Keep the sample compartment clean
The Deuterium lamp used in this unit emits UV radiation
Install the instrument in a ventilated area because it is likely to generate
ozone, which, beyond the limits below, can harm health.
Exposure average value = 100 ppb
Exposure limits value = 200 ppb
It is important that where the equipment and accessories can come into
contact with human or animal tissues or fluids (particularly blood),
they should always be regarded as contaminated and potentially
hazardous (See Section 5 for decontamination)

UVIKON XL/XS
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4.2. DESCRIPTION OF SYMBOLS
Caution (refer to enclosed documents). In the lamp
compartment, it indicates the presence of high temperature and
ozone generated by the Deuterium lamp.
Power on
Power off
AC (alternating current)
DC (direct current)
Protective earth conductor terminal
Caution, risk of electric shock
Caution, hot surface
4.3. QUALITY, RELIABILITY AND SAFETY
This equipment has been designed with emphasis on QUALITY, RELIABILITY and
SAFETY. AQUALABO ANALYSE will accept responsibility for these aspects only if the
following conditions are met:
a) All electrical installations in the room or building in which the equipment is to
be used must comply with regulations specified in the country where the
equipment is to be used.
b) The equipment must be used in accordance with the instructions for use
provided by AQUALABO ANALYSE.
c) All modifications and repairs to the equipment must be carried out by
authorised AQUALABO ANALYSE Personnel, their Agents or authorised
Technicians.
d) Modifications must not be carried out unless they conform to approve
Engineering Service Information issued according to the appropriate
AQUALABO ANALYSE procedure.
e) The equipment installation must be carried out in accordance with local
requirements for responsibility and warranty.

NOTE: Routine tests
Routine test are performed on 100% of the produced equipment according to
the requirements specified in Annex K of the Safety Standard EN 61010-
1+A2: + Dev. UL/CSA

5. INSTRUMENT DESCRIPTION
5.1. GENERAL POINTS
AQUALABO ANALYSE - SECOMAM is proud to introduce the newest series of UVIKON
ultraviolet-visible spectrophotometers: the UVIKON Simplicity Concept with DSP
Technology.
The Advanced Digital Signal Processing Technology inside the UVIKON combines its
high-performance true double beam optical design with rapid scanning and powerful yet
intuitive software.
The UVIKON is engineered to make it quick and easy for all to use – regardless of the
analytical method – without sacrificing capabilities.
The UVIKON Xx spectrophotometer is a double beam spectrophotometer, which employs
state of the art technology for collection, presentation, storage and retrieval of analysis
data.
The UVIKON Xx is completely controlled via the RS-232 interface by an external personal
computer.
The UVIKON Xx software, provided with the spectrophotometer, runs under Windows NT
operating software.
Light sources Deuterium and tungsten-halogen lamps
Monochromator High energy low stray light diffraction grating with 1300
lines/mm, 175mm focal length.
Detector R955 Photomultiplier (Uvikon XL)
Photodiode (Uvikon XS)
Dimensions W 680 mm x D 565 mm x H 275 mm
Weight 35 Kg net
Mains Voltage 100 - 240VAC ±10%
Power 200VA
Frequency 50 - 60 Hz

UVIKON XL/XS
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5.2. SPECIFICATIONS
UVIKON Xx is a double beam high performance spectrophotometer with the following
specifications:
UVIKON XL UVIKON XS
Wavelength range 180-900 nm 190-1100 nm
Wavelength step 0.05-10 nm 0.05-10 nm
Spectral bandwidth 0.2 - 0.5 – 1 – 2 - 4 nm;
0.5– 1–2- 4 (reduced height) 1.8 fixed
Scan speed 10-5000 nm/min 10-2000 nm/min
Transfer speed 7000 nm/min 5000 nm/min
Wavelength accuracy
Holmium peaks,
10nm/min, 0.05nm step
± 0.25 nm ± 0.3 nm
Wavelength precision
Holmium Oxide filter shoulder ± 0.025 nm ± 0.03 nm
Baseline flatness
200-800nm, 2nm, 200nm/min ≤±1mAbs
≤±1mAbs
Stray light
NaI, 220nm, 1nm Bw, ≤0.015 %T ≤0.03 %T
Linearity
1sec, 2nm, r²= 0.999 (250nm) 0-3.3 Abs 0-3.0 Abs
Photometric range ± 5 Abs ± 3.5 Abs
Photometric accuracy
1Abs NIST, 1sec, 590nm ± 3 mAbs ± 3 mAbs
Photometric precision
1Abs NIST, 1sec, 590nm ± 0.5 mAbs ± 0.5 mAbs
Noise RMS
0Abs, 500-600nm, 2nm, 1sec 40 µAbs 30 µAbs
Drift
580nm, 2nm, 1 sec - warm-up 2 hour 0.1 mAbs/h 0.1 mAbs/h
Response time 0.02-5 sec 0.02-5 sec

5.3. SYSTEM CONCEPT
UVIKON Xx is a double beam high performance spectrophotometer controlled from an
external PC running under MS Windows NT.
The communication between PC and spectrophotometer is via a standard RS232
connection
Minimum system requirements to run the UVIKON SW see section 3; as minimum a 17
inch monitor is suggested.
5.3.1. Display
Below is the layout of the UVIKON XL status display with the description of the
symbols.
SYSTEM FAILURE: Red light indicates a system error
MEASURE: Green light shows that UVIKON XL is measuring
HALOGEN LAMP: Green lamp indicates halogen lamp on
DEUTERIUM LAMP: Green light indicates Deuterium lamp on or igniting
POWER ON Green light indicates system power is on
5.3.2. Electronics
Data processing is executed using single channel analogy electronics with 20 bit
A/D converter and DSP processor. High speed microcontrollers control the
photometer drive and the communication with PC. Firmware is stored in Flash
memory and can be upgraded by PC download.
5.3.3. Optics
The basic optical concept is as follows:
Light is provided by D2 and Halogen lamps with a switching mirror and single
monochromator. The beam is split by a rotating chopper wheel which reflects
light via a set of mirrors onto either a photomultiplier detector (XL) or photodiode
(XS).
System failure
Measure
Halogen lamp
Deuterium lamp
Power on

UVIKON XL/XS
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6. INSTALLATION
6.1. UNPACKING AND INSTRUMENT SETUP
6.1.1. Unpacking
The UVIKON Xx is contained in one single packing case. Open the case, lift the
instrument out of the box and place it on a table.
The instrument is heavy, do not lift alone. Have at least one other
person help whenever lifting or moving the instrument.
Most of the accessories are contained in a separate enclosure.
Please make sure that you have removed these before storing the
box, do not discard.
Allow suitable space for connecting and disconnecting the power
cable and switching the instrument on/off.
Check the consignment against the shipping list. If any parts are
damaged, please inform your local AQUALABO ANALYSE representative
immediately.

UVIKON XL/XS
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NOTE: Use only the original packing case to return the unit to the factory for
any reason.
LAB POWER JUNIOR Set up program
APPLICATIONS Set up program
LAB POWER JUNIOR Software User Manual
VALIDATION Software User Manual
DNA/RNA Purity Check - Software User Manual
6.1.2. Checking the serial number of the instrument
The plate showing the serial number is mounted to the rear right hand side of the
instrument. This number must be identical with the serial number on the delivery
note. Should this not be the case please inform the local AQUALABO ANALYSE
representative immediately.
RS232 - PC connection
Mains plug

6.2. INSTALLING THE INSTRUMENT
6.2.1. Environmental conditions
Performance and safety of the equipment are guaranteed only if it is operated
under the following environmental conditions:
The instrument is designed for internal use and altitude below 2000 metres
Installation category II with smaller transient over-voltages (IEC 664)
Pollution degree 2 (CEI EN 61001-1)
Room temperature between 15°C (59°F) and 35°C (95°F)
In locations where the relative humidity is lower than 80%
The UVIKON Xx contains many sensitive optical components, to obtain optimum
performance avoid using this instruments under the following conditions:
In locations subjected to dust or vapours of solvents and acids
In locations prone to vibrations
On an unstable or uneven surface
Near appliances generating strong magnetic fields

UVIKON XL/XS
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6.3. INITIAL INSTALLATION
It is most important that there is sufficient free space around the instrument for
adequate ventilation (10cm).
6.3.1. Connexion of the power supply
The power is supplied via the enclosed plug-in power pack. The power pack supplies the photometer
with low voltage (12 VDC).
Warning
The line voltage of the usage location must fulfil the specifications stated on the power pack.
Always use the supplied 12 V original power pack only. The outlet must have a protective earth
connection.
1) Connect the miniplug of the power pack to the main plug of the spectrophotometer.
2) Connect the power pack to an easily accessible power socket.
The main switch is located in the lower left side of the equipment
6.3.2. Connection to the Personal Computer
Use PC COM1 for the connection of the spectrophotometer serial cable and
COM2 for external UVIKON XL accessories (see section 6). Connect the serial
cable between UVIKON Xx 9 poles DIN connector located on the rear right hand
side and PC serial port.
Baud rate must be set to 38400 bit/sec
NOTE: Use only equipment, e.g. PC, monitor, keyboard etc., which
comply with the standard EN60950
Refer to the specific documentation for hardware connection and software
installation
6.3.2.1. PC minimum requirements
CPU: 300 MHz
RAM: 64 MB
Hard Disk 2 GB
CD ROM drive
Operating system Windows NT 4.0 + Service pack 4 only
6.3.3. Software Installation, LabPower JUNIOR
1. Insert the CD-ROM (70/91-91640)
2. Select Run from the Start menu
3. Select the Set up program from the CD_ROM Lab Power Junior directory
4. Press OK to start the Set up program
5. Follow the on-screen instructions